FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 7-9252.34200000001b6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 7-9252.34200000001b6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9407964
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG1502.5710027E-514.25014732-33
CGATCTA4300.013.80813634-35
TCCGATC8750.013.02870732-33
CCGATCT8700.012.0116232-33
CTTCCGA11800.011.87480730-31
GACCGGA4154.180025E-511.4459639
GACGTGT11850.010.42202120-21
TTCCGAT12050.09.85461130-31
CCGTTAA4358.1904E-49.8250244
TCAGACG16900.09.13478318-19
GACGTTG4504.2291504E-88.97229120-21
CGTGATG29900.08.8939541
TCCGACG2150.0081886558.83737218-19
CAGACGT14900.08.44814218-19
CCGACGT3100.00233602947.66143118-19
GTGACTG27550.07.58434967
TAAGACG8157.023118E-96.70257718-19
TCCGATA3900.0029084146.698787732-33
TGACTGG38250.06.33286058
AGACGTG17000.06.28681320-21
Produced by FastQC (version 0.11.2)