##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n02 7-9252.34200000001b6c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9407964 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.01675420951866 38.0 32.0 38.0 32.0 38.0 2 35.15560369916381 38.0 32.0 38.0 32.0 38.0 3 35.51982437432796 38.0 32.0 38.0 32.0 38.0 4 35.318303513916504 38.0 32.0 38.0 32.0 38.0 5 35.492910261986545 38.0 32.0 38.0 32.0 38.0 6 37.48999772958315 40.0 38.0 40.0 32.0 40.0 7 37.701647030111936 40.0 38.0 40.0 32.0 40.0 8 37.78483516731144 40.0 38.0 40.0 32.0 40.0 9 37.82143235241971 40.0 38.0 40.0 32.0 40.0 10-11 37.84087959945425 40.0 38.0 40.0 32.0 40.0 12-13 37.82065014279391 40.0 38.0 40.0 32.0 40.0 14-15 37.77611048469148 40.0 38.0 40.0 32.0 40.0 16-17 37.770679075727756 40.0 38.0 40.0 32.0 40.0 18-19 37.749479696138295 40.0 38.0 40.0 32.0 40.0 20-21 37.72426371954654 40.0 38.0 40.0 32.0 40.0 22-23 37.71272115837178 40.0 38.0 40.0 32.0 40.0 24-25 37.65998955778318 40.0 38.0 40.0 32.0 40.0 26-27 37.64075176095487 40.0 38.0 40.0 32.0 40.0 28-29 37.56770997423034 40.0 38.0 40.0 32.0 40.0 30-31 37.528120377586475 40.0 38.0 40.0 32.0 40.0 32-33 37.530937246358505 40.0 38.0 40.0 32.0 40.0 34-35 37.45644201019476 40.0 38.0 40.0 32.0 40.0 36-37 37.458675277669 40.0 38.0 40.0 32.0 40.0 38-39 37.41545715948743 40.0 38.0 40.0 32.0 40.0 40-41 37.31111917520093 40.0 38.0 40.0 32.0 40.0 42-43 37.079266194045815 39.0 38.0 40.0 32.0 40.0 44-45 36.99004954738347 38.0 38.0 40.0 32.0 40.0 46-47 36.915471827910906 38.0 38.0 40.0 32.0 40.0 48-49 36.83376583924003 38.0 38.0 40.0 32.0 40.0 50-51 36.773625249841515 38.0 38.0 40.0 32.0 40.0 52-53 36.70485346244948 38.0 38.0 40.0 32.0 40.0 54-55 36.65447433684908 38.0 38.0 40.0 32.0 40.0 56-57 36.63326204266938 38.0 38.0 40.0 32.0 40.0 58-59 36.56614943467046 38.0 38.0 40.0 32.0 40.0 60-61 36.52543116661586 38.0 38.0 40.0 32.0 40.0 62-63 36.456802130620396 38.0 38.0 40.0 32.0 40.0 64-65 36.438322202338355 38.0 38.0 40.0 32.0 40.0 66-67 36.36794608270185 38.0 38.0 40.0 27.0 40.0 68-69 36.3147495568648 38.0 38.0 40.0 27.0 40.0 70-71 36.207883023361916 38.0 38.0 40.0 27.0 40.0 72-73 36.13771640707809 38.0 38.0 40.0 27.0 40.0 74-75 36.04971963115506 38.0 38.0 40.0 27.0 40.0 76-77 35.96726502142228 38.0 38.0 40.0 27.0 40.0 78-79 35.88079572795984 38.0 38.0 40.0 27.0 40.0 80-81 35.80259092190404 38.0 38.0 40.0 27.0 40.0 82-83 35.71274422393623 38.0 38.0 40.0 27.0 40.0 84-85 35.61039689352553 38.0 38.0 40.0 27.0 40.0 86-87 35.540139449938366 38.0 38.0 40.0 27.0 40.0 88-89 35.47091076241363 38.0 38.0 40.0 27.0 40.0 90-91 35.34749032840686 38.0 38.0 40.0 27.0 40.0 92-93 35.246438389857786 38.0 38.0 40.0 27.0 40.0 94-95 35.142810601741246 38.0 38.0 40.0 27.0 40.0 96-97 35.060480886193865 38.0 38.0 40.0 27.0 40.0 98-99 34.92210960841261 38.0 35.0 40.0 27.0 40.0 100-101 32.931886325245294 38.0 29.5 39.0 20.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.0677417349907472 2201 2 0.19610182926288644 2201 3 0.3392805180780343 2201 4 0.21953557663764656 2201 5 0.23395917267164634 2201 6 0.28889946828185487 2201 7 0.22981248502164675 2201 8 0.1306717924384202 2201 9 0.18061612728522647 2201 10-11 0.11589601515883885 2201 12-13 0.21537467344706585 2201 14-15 0.17096540203464627 2201 16-17 0.1399870220526438 2201 18-19 0.16264950509503961 2201 20-21 0.1934246358162639 2201 22-23 0.19971752725285796 2201 24-25 0.2052359705965685 2201 26-27 0.21778429936246368 2201 28-29 0.21802193922904678 2201 30-31 0.20366144511378081 2201 32-33 0.17316914921175197 2201 34-35 0.18340002696490387 2201 36-37 0.10913122022625288 2201 38-39 0.21789389062116982 2201 40-41 0.126669541318833 2201 42-43 0.18260833412171706 2201 44-45 0.15280399840120396 2201 46-47 0.15809134020331328 2201 48-49 0.10557604897002193 2201 50-51 0.19988108336113442 2201 52-53 0.18449918953275812 2201 54-55 0.25715839217865977 2201 56-57 0.0663611273287188 2201 58-59 0.10468100863256069 2201 60-61 0.037388956818382724 2201 62-63 0.16077272624360717 2201 64-65 0.12673785564431483 2201 66-67 0.16516275541304992 2201 68-69 0.07932889800247267 2201 70-71 0.18888111163773402 2201 72-73 -0.05596540441421638 2201 74-75 0.05508053779121269 2201 76-77 0.07627079572992557 2201 78-79 0.07898692966719523 2201 80-81 0.20426310528223013 2201 82-83 0.09771762217662427 2201 84-85 0.016685685420853247 2201 86-87 0.19239996334653853 2201 88-89 0.12296680283328243 2201 90-91 0.051628319019016544 2201 92-93 -0.09750552139731639 2201 94-95 0.06311869966579309 2201 96-97 -0.16202102897658222 2201 98-99 0.001895337837936495 2201 100-101 0.08623244338100733 1101 1 0.01648972643772595 1101 2 0.1879078173962796 1101 3 0.22398003430351565 1101 4 0.16127747169930728 1101 5 0.28562897557284117 1101 6 0.2684467992318247 1101 7 0.31545303331012775 1101 8 0.2674776374924477 1101 9 0.30473915081376646 1101 10-11 0.30042878419146035 1101 12-13 0.2794146411587306 1101 14-15 0.28324901283446025 1101 16-17 0.2788490265048438 1101 18-19 0.25931260768573594 1101 20-21 0.2978255264658074 1101 22-23 0.27210934257929154 1101 24-25 0.2865507430988501 1101 26-27 0.24661692779890387 1101 28-29 0.3557722749974488 1101 30-31 0.30867708272866423 1101 32-33 0.2530325025641673 1101 34-35 0.28992891129635723 1101 36-37 0.2554431845956131 1101 38-39 0.2340362309565336 1101 40-41 0.2882133035140839 1101 42-43 0.2829675487489993 1101 44-45 0.2485827127739455 1101 46-47 0.2213119846347098 1101 48-49 0.22115302190427144 1101 50-51 0.2417564739856246 1101 52-53 0.253208235111984 1101 54-55 0.20578370068555074 1101 56-57 0.2181795979005159 1101 58-59 0.20625999198402667 1101 60-61 0.1954623751421849 1101 62-63 0.21503146357241576 1101 64-65 0.21868907440123309 1101 66-67 0.23701285611427636 1101 68-69 0.16565440721785762 1101 70-71 0.2694731778895445 1101 72-73 0.24219239596836672 1101 74-75 0.25270445281446996 1101 76-77 0.227976452596792 1101 78-79 0.15865000792360462 1101 80-81 0.3069772077986386 1101 82-83 0.27663806323761975 1101 84-85 0.16487170788792582 1101 86-87 0.2805379682365512 1101 88-89 0.19754094991908744 1101 90-91 0.20507321990403682 1101 92-93 0.17328559367758345 1101 94-95 0.21068366082103296 1101 96-97 0.2620717963290673 1101 98-99 0.1309329949701521 1101 100-101 0.2916242811824503 2202 1 -0.08481754877092129 2202 2 0.03770866952751106 2202 3 0.17095726749315077 2202 4 -0.0067591846918801934 2202 5 0.11805501155858877 2202 6 0.12904836840867517 2202 7 0.18268957252397655 2202 8 0.183640005485195 2202 9 0.25048428904367626 2202 10-11 0.18452309022901403 2202 12-13 0.2166629916330578 2202 14-15 0.22947112812434511 2202 16-17 0.19150065893624912 2202 18-19 0.20610165817433312 2202 20-21 0.1912716014810698 2202 22-23 0.19641140651201283 2202 24-25 0.2263962314544301 2202 26-27 0.22597010143710605 2202 28-29 0.16490756471895196 2202 30-31 0.18011796348710618 2202 32-33 0.2112087188561489 2202 34-35 0.14660345565206967 2202 36-37 0.21437133915078022 2202 38-39 0.24905594997632363 2202 40-41 0.15040107559680393 2202 42-43 0.04842103555708377 2202 44-45 0.12430581880637703 2202 46-47 0.02938102254571362 2202 48-49 0.09085751166034584 2202 50-51 0.17659659867006638 2202 52-53 0.18065432178485707 2202 54-55 0.1486864995873205 2202 56-57 0.11404891909408832 2202 58-59 0.13138479240341638 2202 60-61 0.07224027745240136 2202 62-63 0.1588735814248281 2202 64-65 0.022029364392047057 2202 66-67 0.04379574208643078 2202 68-69 0.05479026857928204 2202 70-71 0.07347193998472079 2202 72-73 0.015260376642309836 2202 74-75 0.03955791674054154 2202 76-77 0.025178768375042182 2202 78-79 0.056575212022330845 2202 80-81 0.0977190483317969 2202 82-83 0.011680173466480426 2202 84-85 0.07076563062739893 2202 86-87 0.11194554484380603 2202 88-89 -0.003175272215962366 2202 90-91 0.08223925258737808 2202 92-93 0.049352637239692854 2202 94-95 0.14496718807252051 2202 96-97 0.13192368923681386 2202 98-99 0.030768649123473324 2202 100-101 0.24843431784072578 1102 1 -0.213923221215218 1102 2 -0.12038629789972077 1102 3 -0.0035744638507608784 1102 4 -0.11788461166666764 1102 5 0.021862442124522374 1102 6 -0.07570896012611428 1102 7 0.1446537992827146 1102 8 0.1512703473636421 1102 9 0.20104617245166878 1102 10-11 0.0952374381231138 1102 12-13 0.22999406933855226 1102 14-15 0.19979190858109064 1102 16-17 0.16062322270658314 1102 18-19 0.15934967567738312 1102 20-21 0.16536706925869282 1102 22-23 0.18818135869028652 1102 24-25 0.1725018078097449 1102 26-27 0.17228976919579253 1102 28-29 0.06472589690542918 1102 30-31 0.13664716411763322 1102 32-33 0.12868662026917832 1102 34-35 0.19421491031459226 1102 36-37 0.11986611163054306 1102 38-39 0.15305948746085107 1102 40-41 0.10790730392336201 1102 42-43 -0.00866551465333032 1102 44-45 -0.06186710350134206 1102 46-47 -0.01843007696685106 1102 48-49 -0.059738294923874946 1102 50-51 0.013396697220699139 1102 52-53 0.02955391617840064 1102 54-55 0.0171050510026447 1102 56-57 -0.09168995886138731 1102 58-59 -0.06043823122910652 1102 60-61 -0.015223746517698089 1102 62-63 -0.042971089196328194 1102 64-65 -0.056223226818417515 1102 66-67 -0.08276710110649788 1102 68-69 -0.250053693965242 1102 70-71 -0.026246770880213433 1102 72-73 -0.11888283533378541 1102 74-75 -0.022940185112695133 1102 76-77 -0.08554129855418324 1102 78-79 -0.07242667178960716 1102 80-81 -0.005088369776899526 1102 82-83 -0.06359515223688561 1102 84-85 -0.014896745209128426 1102 86-87 -0.03655959564331823 1102 88-89 -0.1700276999379966 1102 90-91 -0.26940949763243793 1102 92-93 -0.1564679855525597 1102 94-95 -0.038555621868326284 1102 96-97 -0.07810357126970047 1102 98-99 -0.160316442701955 1102 100-101 -0.11020107730242756 2203 1 -0.27792949581337467 2203 2 -0.18199470448485044 2203 3 -0.043259717959408306 2203 4 -0.16469695115448246 2203 5 -0.12202253935624441 2203 6 -0.14299540341744432 2203 7 0.04865355911906022 2203 8 0.03392253047184823 2203 9 0.16753708398513112 2203 10-11 0.07034157854623402 2203 12-13 0.16026043218918318 2203 14-15 0.11902184495879453 2203 16-17 0.151211769898012 2203 18-19 0.15583196730330684 2203 20-21 0.22114007982931838 2203 22-23 0.06798992083631106 2203 24-25 0.13499854656289045 2203 26-27 0.05170000779225603 2203 28-29 0.08685906777396468 2203 30-31 0.07068019935653069 2203 32-33 0.07774319560097354 2203 34-35 0.04823424402458443 2203 36-37 0.05586412001584051 2203 38-39 0.06683051028880982 2203 40-41 0.061124196482552406 2203 42-43 0.019571014182602653 2203 44-45 -0.09358465986512954 2203 46-47 -0.1735147923096605 2203 48-49 -0.13358359314743495 2203 50-51 -0.07953102436811577 2203 52-53 -0.12710614866651326 2203 54-55 -0.059944646375193145 2203 56-57 -0.2568374662118913 2203 58-59 -0.07553892590527767 2203 60-61 -0.17859687805170665 2203 62-63 -0.10696623473442202 2203 64-65 -0.1905278592190527 2203 66-67 -0.1599843401520502 2203 68-69 -0.10688103625400913 2203 70-71 -0.1337779348967345 2203 72-73 -0.14416267183547404 2203 74-75 -0.15229949060382353 2203 76-77 -0.10247409039177313 2203 78-79 -0.12179807410633714 2203 80-81 -0.11957923579566909 2203 82-83 -0.22171694728219649 2203 84-85 -0.24041041738727387 2203 86-87 -0.3248948850246407 2203 88-89 -0.28108194508326534 2203 90-91 -0.08386407609670954 2203 92-93 -0.21522998891048672 2203 94-95 -0.20137208427107822 2203 96-97 -0.28464462518511624 2203 98-99 -0.39016093563938625 2203 100-101 -0.09938453804340952 1103 1 -0.34302638370341043 1103 2 -0.23543581929633461 1103 3 -0.05502905964396376 1103 4 -0.10961851394871758 1103 5 -0.03744666502969096 1103 6 -0.1397274083619635 1103 7 0.0320722752578817 1103 8 0.04239297676775067 1103 9 0.053097293191314066 1103 10-11 0.0783758221140971 1103 12-13 0.05557176002114517 1103 14-15 0.07664953640048111 1103 16-17 0.01588378983233696 1103 18-19 0.015074811487146178 1103 20-21 0.050160811302284 1103 22-23 0.0872865860067833 1103 24-25 0.08614754952725434 1103 26-27 0.017648299839699177 1103 28-29 0.024782338689320227 1103 30-31 0.035443554304990244 1103 32-33 0.04108590831307879 1103 34-35 0.08757429754098212 1103 36-37 0.03707019559330149 1103 38-39 0.033730917398955285 1103 40-41 -0.012114958261967956 1103 42-43 -0.10230729942183103 1103 44-45 -0.14554512208668058 1103 46-47 -0.17570650713748392 1103 48-49 -0.13269134893342027 1103 50-51 -0.14979322613426405 1103 52-53 -0.13547726166791563 1103 54-55 -0.014254777836470112 1103 56-57 -0.0698605150935947 1103 58-59 -0.09925432128132172 1103 60-61 -0.17644938627406503 1103 62-63 -0.1954146776215282 1103 64-65 -0.16768678749132704 1103 66-67 -0.29388962028426135 1103 68-69 -0.19982962207550514 1103 70-71 -0.13392517649560176 1103 72-73 -0.12641141858748028 1103 74-75 -0.2149971036402789 1103 76-77 -0.3114547758234707 1103 78-79 -0.2765874688050829 1103 80-81 -0.16864280340769966 1103 82-83 -0.3133887843488523 1103 84-85 -0.27675876528317644 1103 86-87 -0.3563008071699798 1103 88-89 -0.28400819965946056 1103 90-91 -0.21763881452794465 1103 92-93 -0.17776769446658136 1103 94-95 -0.11720649382651516 1103 96-97 -0.2661819434988857 1103 98-99 -0.11969438827264867 1103 100-101 -0.07943972152779821 2204 1 -0.1009393323317056 2204 2 -0.06577544950944514 2204 3 0.09282090634378193 2204 4 -0.04777138379994739 2204 5 0.020982874251117778 2204 6 -0.014791396152197933 2204 7 0.09469102922526673 2204 8 0.14468806215684538 2204 9 0.168355825939436 2204 10-11 0.18707009361336446 2204 12-13 0.23779703354397697 2204 14-15 0.14779628459237415 2204 16-17 0.15864777768267402 2204 18-19 0.19352056649061922 2204 20-21 0.13418326731325436 2204 22-23 0.1366989409164745 2204 24-25 0.12275630565684992 2204 26-27 0.12285983289266511 2204 28-29 0.17272385280264757 2204 30-31 0.1534948813621142 2204 32-33 0.16018977106586618 2204 34-35 0.09827354894353846 2204 36-37 0.11663833859416428 2204 38-39 0.15858487016167544 2204 40-41 0.01729563934336653 2204 42-43 0.039771074713179644 2204 44-45 0.05456039267426149 2204 46-47 0.046740911294442355 2204 48-49 0.025173098968920726 2204 50-51 -0.022534594155267484 2204 52-53 0.05611148791221865 2204 54-55 -0.035956669730886404 2204 56-57 0.01540584847813875 2204 58-59 0.07901300394462396 2204 60-61 0.028322446388379774 2204 62-63 0.06987827993208384 2204 64-65 0.03960896262436364 2204 66-67 1.9238135097054965E-4 2204 68-69 0.00841618163633484 2204 70-71 0.10346487518368264 2204 72-73 0.07174610571602358 2204 74-75 -0.028275390434643555 2204 76-77 0.06337433097249345 2204 78-79 0.007344447235333007 2204 80-81 0.05303357041661627 2204 82-83 0.0733946366866789 2204 84-85 0.06376583705977623 2204 86-87 0.042286570936838075 2204 88-89 0.04989614921529295 2204 90-91 0.05102687782565596 2204 92-93 0.08889581021794868 2204 94-95 0.035216211966478284 2204 96-97 0.04310233364877547 2204 98-99 -0.0010679538579481118 2204 100-101 0.10820478957084134 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1215 50-51 0.01599116506739051 1215 52-53 0.04120989231642369 1215 54-55 0.06880251467656961 1215 56-57 0.00825638059917111 1215 58-59 0.10421615316221988 1215 60-61 0.004449592179746276 1215 62-63 -0.03138171834313397 1215 64-65 -0.10949382678824549 1215 66-67 -0.04118191071592037 1215 68-69 -0.0386802546149454 1215 70-71 0.014674977994054927 1215 72-73 0.06671603983498642 1215 74-75 0.10430061396419887 1215 76-77 0.10652733097919054 1215 78-79 0.018421491150043323 1215 80-81 0.059832267882384826 1215 82-83 0.19085469491859186 1215 84-85 0.11378691267934471 1215 86-87 0.13370767417884366 1215 88-89 0.09608569621807561 1215 90-91 0.10723148316517239 1215 92-93 0.15970936025703963 1215 94-95 0.1503586912383028 1215 96-97 0.1057389150540331 1215 98-99 0.052695348627452177 1215 100-101 0.05413727064309626 1212 1 0.22606636452577789 1212 2 0.08745892031047475 1212 3 -0.07325260123452182 1212 4 0.04444815513880229 1212 5 -0.02986423888680889 1212 6 -0.13143320859899887 1212 7 -0.28451111546166885 1212 8 -0.36165546837275997 1212 9 -0.41322539765631205 1212 10-11 -0.40051291119713284 1212 12-13 -0.4779811855689644 1212 14-15 -0.4317311099552512 1212 16-17 -0.36118120176276136 1212 18-19 -0.3762687068841615 1212 20-21 -0.353033845953469 1212 22-23 -0.31999277901432066 1212 24-25 -0.2659822437729531 1212 26-27 -0.23177826157722592 1212 28-29 -0.18574085838341148 1212 30-31 -0.2168020213256554 1212 32-33 -0.21006905530724396 1212 34-35 -0.20626596041995526 1212 36-37 -0.16748044450730504 1212 38-39 -0.17859369972487826 1212 40-41 -0.15196546653375265 1212 42-43 0.021346978375632375 1212 44-45 -0.026666534114980323 1212 46-47 -0.020809010463942457 1212 48-49 0.04011168243630436 1212 50-51 -0.10568252096363295 1212 52-53 -0.06641597214308348 1212 54-55 -0.027080615622544713 1212 56-57 0.031661211726628835 1212 58-59 0.016418547775629122 1212 60-61 0.030478117726993048 1212 62-63 0.00569628566174174 1212 64-65 -0.06704194399211616 1212 66-67 0.02024775318398042 1212 68-69 0.02637964175768559 1212 70-71 -0.04886585091110618 1212 72-73 0.04604796245569531 1212 74-75 0.04826940884466069 1212 76-77 0.09418418984798649 1212 78-79 0.07482768465206391 1212 80-81 -0.013231400583137543 1212 82-83 -0.01952636819568454 1212 84-85 -0.0026210809986579875 1212 86-87 -0.07163008039130858 1212 88-89 -0.08390026620273972 1212 90-91 -0.09867846182427797 1212 92-93 -0.12054371620750715 1212 94-95 -0.043845052472505586 1212 96-97 -0.04942643990394657 1212 98-99 -0.009045362921426658 1212 100-101 -0.1517236592098783 1213 1 0.2744377761509895 1213 2 0.2378520903099357 1213 3 -0.10520990382408257 1213 4 0.12478854142337781 1213 5 -0.004271618690786738 1213 6 0.10162777579489557 1213 7 -0.10077691642148778 1213 8 -0.3639908356802053 1213 9 -0.35935478890137773 1213 10-11 -0.37075243933270485 1213 12-13 -0.2806552090922736 1213 14-15 -0.2696789271587079 1213 16-17 -0.26692673238584774 1213 18-19 -0.1739096769029871 1213 20-21 -0.25460429750069835 1213 22-23 -0.2770345200429247 1213 24-25 -0.41012780797191795 1213 26-27 -0.3061058345701966 1213 28-29 -0.3384571825311866 1213 30-31 -0.55333418136604 1213 32-33 -0.4596877387069185 1213 34-35 -0.5297492343729218 1213 36-37 -0.576689558778277 1213 38-39 -0.710570106310584 1213 40-41 -0.45235633303865086 1213 42-43 -0.10966364881846857 1213 44-45 -0.22752669995537644 1213 46-47 -0.29238877048683065 1213 48-49 -0.21757764782773847 1213 50-51 -0.4100994109278844 1213 52-53 -0.4057069324876892 1213 54-55 -0.1883232949657483 1213 56-57 -0.2789817386984623 1213 58-59 -0.3785928414783797 1213 60-61 -0.2789100529871362 1213 62-63 -0.3643859152763014 1213 64-65 -0.5105602635923177 1213 66-67 -0.2839180552280496 1213 68-69 -0.2672275282530805 1213 70-71 -0.3511293486726643 1213 72-73 -0.2415708785555708 1213 74-75 -0.3324780202611777 1213 76-77 -0.28086439626981985 1213 78-79 -0.33058550439645273 1213 80-81 -0.2203991703344883 1213 82-83 -0.20866066933040628 1213 84-85 -0.130510218610425 1213 86-87 -0.16229418695822773 1213 88-89 -0.1903606898438568 1213 90-91 -0.2509056363880475 1213 92-93 -0.30139782880429067 1213 94-95 -0.3641590824891381 1213 96-97 -0.32676641951479724 1213 98-99 -0.2988746214752851 1213 100-101 -1.0403786676045215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44.0 3 54.0 4 59.0 5 74.0 6 92.0 7 63.0 8 38.0 9 44.0 10 41.0 11 60.0 12 64.0 13 105.0 14 384.0 15 3058.0 16 14619.0 17 31148.0 18 39030.0 19 39697.0 20 41570.0 21 46261.0 22 52681.0 23 60174.0 24 67469.0 25 75801.0 26 85313.0 27 95637.0 28 108575.0 29 124815.0 30 143823.0 31 167553.0 32 197538.0 33 238768.0 34 297405.0 35 393003.0 36 567903.0 37 977704.0 38 2370234.0 39 3167063.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.753543482094372 31.012051883591113 29.380031073861595 19.854373560452927 2 21.4644522645637 31.379006235911483 28.480067925697128 18.676473573827685 3 21.203839268422467 29.673992972239944 29.270286869432432 19.851880889905157 4 21.763867091066505 28.772799577371742 29.03836658855768 20.424966743004074 5 21.64446984342422 29.553824824852278 29.020541920966508 19.781163410756992 6 21.867287467916714 29.312862823873193 28.306587342219984 20.513262365990105 7 22.03092224131501 29.787272720314434 28.603519707034096 19.578285331336456 8 20.966584948057196 29.59131411475487 29.261551780478484 20.18054915670945 9 21.17737960369152 29.087657989893835 29.432470474786566 20.30249193162808 10-11 21.370504332995957 29.391855171970636 28.73646724381127 20.501173251222134 12-13 21.098622091513057 29.53693028507731 29.093211637811923 20.27123598559771 14-15 21.23785424477013 29.29531386666661 28.992375748107918 20.47445614045534 16-17 21.40923804761685 29.259207305790834 28.9707810996479 20.36077354694442 18-19 21.17403613234233 29.03697250697422 29.110830447864366 20.678160912819084 20-21 21.502456159673393 28.98517714254975 28.729759287102937 20.78260741067392 22-23 21.448762317245233 28.868803591855464 28.781749347465308 20.900684743434 24-25 21.667486930794905 28.751049783919147 28.717362073091863 20.86410121219409 26-27 21.861227356437052 28.82981953617495 28.447356407283365 20.861596700104638 28-29 21.646289338775983 28.922240448499686 28.5060674790946 20.925402733629735 30-31 21.88818044699304 28.556409063498368 28.548142106387708 21.007268383120884 32-33 21.90715099614523 28.845848753335122 28.37536413604961 20.87163611447004 34-35 21.95568386790285 28.672074049775713 28.2923463304336 21.079895751887832 36-37 21.935807299627072 28.798530611810925 28.26217734197033 21.003484746591674 38-39 21.82616243674036 28.630187322320662 28.47997923720767 21.063671003731304 40-41 22.130326296871132 28.74638103307796 28.182795615463878 20.94049705458703 42-43 22.05697775183669 28.740582214495426 28.186862390261304 21.01557764340658 44-45 21.948406624508948 28.508797577880422 28.30784207569421 21.234953721916412 46-47 22.10823543671514 28.774296064040282 28.055193172382044 21.062275326862533 48-49 21.950323991189137 28.555170518978617 28.232962787088113 21.261542702744137 50-51 22.04102976938811 28.71015232369272 27.982472001365714 21.26634590555345 52-53 21.9685910467312 28.75586372760832 28.178762354762434 21.09678287089805 54-55 22.231118155556167 28.401821298810166 28.11148780604115 21.255572739592516 56-57 22.220329748637514 28.69898448060026 27.976596480881575 21.10408928988065 58-59 21.986011767415796 28.64537884207391 28.132872846277916 21.235736544232374 60-61 22.12154958605958 28.529429480949375 27.837452712289174 21.511568220701875 62-63 22.08363115276788 28.533580077122494 28.116522114039967 21.266266656069654 64-65 22.191627614018213 28.421200197506668 27.987655195203704 21.39951699327142 66-67 22.153203969871708 28.58596923294141 27.803109494682126 21.45771730250475 68-69 21.98041157821834 28.510186749092604 28.030162074047677 21.479239598641385 70-71 22.297059395962396 28.546873660143667 27.722049796178368 21.43401714771557 72-73 22.221505661840126 28.636262306680887 27.83542190009023 21.306810131388758 74-75 22.14828521860731 28.515342923583177 27.887395257216014 21.448976600593504 76-77 22.232410871851517 28.56915313784949 27.82846229282257 21.36997369747643 78-79 22.19377356710036 28.493989018744692 27.994236901111552 21.318000513043398 80-81 22.438130906314566 28.46709902989431 27.682667083504803 21.41210298028632 82-83 22.219938907719882 28.743018706832395 27.86537877803849 21.171663607409233 84-85 22.245310008070415 28.54223345588724 27.742082958427844 21.470373577614502 86-87 22.11479378767135 28.670180443896363 27.813960447780083 21.4010653206522 88-89 22.150427604752437 28.45100748053747 27.898622528477073 21.499942386233016 90-91 22.25377424802044 28.378914353779518 27.781918523280112 21.585392874919926 92-93 22.093574655994612 28.516440761223695 27.85466594449525 21.535318638286448 94-95 22.20224938728654 28.45834215804139 27.750295263254753 21.589113191417315 96-97 22.234068342875606 28.613074923179976 27.6483280388264 21.504528695118026 98-99 22.14604051267675 28.570494967288763 27.749028914958433 21.53443560507606 100-101 22.52286031707609 28.289526721402243 27.3396608447634 21.84795211675826 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 130.0 1 137.0 2 171.0 3 228.0 4 297.5 5 342.0 6 368.0 7 437.5 8 525.5 9 689.0 10 866.0 11 1131.0 12 1498.0 13 1949.5 14 2542.5 15 3177.0 16 4396.5 17 6225.0 18 8103.0 19 10242.0 20 13321.5 21 16757.5 22 20801.5 23 25654.0 24 30813.0 25 36724.5 26 44049.5 27 51611.0 28 60015.0 29 71280.5 30 86730.5 31 107501.5 32 136875.0 33 176341.0 34 224721.5 35 287119.0 36 364005.5 37 440360.5 38 501152.0 39 542021.0 40 556201.5 41 538099.0 42 493860.5 43 439463.0 44 382964.0 45 338305.5 46 308426.0 47 288280.0 48 277237.0 49 265242.5 50 250498.0 51 234240.5 52 215642.0 53 196663.0 54 180366.5 55 165513.0 56 153809.0 57 142356.5 58 127059.0 59 110614.5 60 95046.0 61 78352.5 62 62727.0 63 50739.0 64 40359.5 65 31554.5 66 24877.5 67 19225.5 68 14283.0 69 10777.5 70 8190.0 71 6149.0 72 4670.5 73 3587.5 74 2750.0 75 2099.5 76 1600.0 77 1248.0 78 998.5 79 737.0 80 500.5 81 368.0 82 258.0 83 184.0 84 145.0 85 103.5 86 71.0 87 46.0 88 32.5 89 24.0 90 21.5 91 18.0 92 15.0 93 12.0 94 11.0 95 9.5 96 6.5 97 6.0 98 5.0 99 10.5 100 32.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.058696653175969E-4 2 3.932838178377383E-4 3 0.0013818080086190805 4 0.001796350411204805 5 0.00809952078898261 6 0.012712633679295543 7 0.013520459899719006 8 0.009417553043357734 9 0.0025722887545062884 10-11 0.00593645979087505 12-13 0.010517684804066002 14-15 0.0344176487069891 16-17 0.014865065385029108 18-19 0.021997320567978363 20-21 0.017145048599250593 22-23 0.03091529686975843 24-25 0.026116171362900623 26-27 0.022215221061645217 28-29 0.029124261104740624 30-31 0.032445914971613414 32-33 0.037330074817463164 34-35 0.01860657630067462 36-37 0.028582167193666984 38-39 0.02846524497755306 40-41 0.031898506414352774 42-43 0.03985453175628648 44-45 0.028146366206333272 46-47 0.02799755611309737 48-49 0.02206109632222232 50-51 0.03564533197618528 52-53 0.02852902073179702 54-55 0.03101096050112437 56-57 0.024282618428386842 58-59 0.02567505572937992 60-61 0.03367891288699659 62-63 0.029724816123871223 64-65 0.02882664091826882 66-67 0.02465995830766359 68-69 0.026679523858722246 70-71 0.029809850462863163 72-73 0.03686770059919447 74-75 0.03146802007320606 76-77 0.03362045177893963 78-79 0.03509260877273765 80-81 0.04075270696188889 82-83 0.042448079095540756 84-85 0.0476511177126103 86-87 0.04887348633561948 88-89 0.05133416752019884 90-91 0.05101528874897906 92-93 0.051456404382499765 94-95 0.052237657371988246 96-97 0.052928561376297784 98-99 0.05399680525988407 100-101 0.05767454042128563 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9407964.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.91103268458647 #Duplication Level Percentage of deduplicated Percentage of total 1 44.941089788906716 6.7011805872332335 2 2.0814991895784387 0.6207460489748869 3 2.4155754461439836 1.0805617328861246 4 2.6041078974582543 1.5531975189275913 5 3.4847494039637916 2.5980606130048662 6 3.753134043590358 3.357786263616601 7 3.9573810507778493 4.130604673545816 8 4.205030237929991 5.016107465395882 9 3.7765233550418964 5.068067686302048 >10 28.403452477334262 65.47813066303259 >50 0.3196632779582068 3.1021980752096456 >100 0.05692623049584832 1.161771840817384 >500 5.400594596674329E-4 0.07083034441673772 >1k 3.275413607455432E-4 0.060756486636555695 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0629292373992927E-5 0.0 0.0 2 1.0629292373992927E-5 0.0 0.0 3 1.0629292373992927E-5 0.0 0.0 4 1.0629292373992927E-5 0.0 0.0 5 1.0629292373992927E-5 0.0 0.0 6 1.0629292373992927E-5 0.0 0.0 7 1.0629292373992927E-5 0.0 0.0 8 1.0629292373992927E-5 0.0 0.0 9 1.0629292373992927E-5 0.0 0.0 10-11 2.1258584747985854E-5 0.0 0.0 12-13 8.503433899194342E-5 0.0 0.0 14-15 1.7538332417088332E-4 0.0 0.0 16-17 3.295080635937808E-4 0.0 0.0 18-19 4.0391311021173123E-4 0.0 0.0 20-21 4.942620953906711E-4 0.0 0.0 22-23 6.111843115045934E-4 0.0 0.0 24-25 8.237701589844518E-4 0.0 0.0 26-27 0.001089502468334275 0.0 0.0 28-29 0.00130740296200113 0.0 0.0 30-31 0.001525303455667985 1.0629292373992927E-5 0.0 32-33 0.0018229236421397873 1.0629292373992927E-5 1.594393856098939E-4 34-35 0.0020355094896196456 1.0629292373992927E-5 1.594393856098939E-4 36-37 0.002545715523571306 1.0629292373992927E-5 1.594393856098939E-4 38-39 0.0028592796486040977 1.0629292373992927E-5 1.594393856098939E-4 40-41 0.003268507405002825 1.0629292373992927E-5 1.594393856098939E-4 42-43 0.003778713438954486 4.2517169495971715E-5 1.594393856098939E-4 44-45 0.004156053318231235 7.44050466179505E-5 1.594393856098939E-4 46-47 0.004581225013190951 7.44050466179505E-5 1.594393856098939E-4 48-49 0.00515520680138657 7.44050466179505E-5 1.594393856098939E-4 50-51 0.005553805265411304 7.44050466179505E-5 1.594393856098939E-4 52-53 0.005877998682818089 7.44050466179505E-5 1.594393856098939E-4 54-55 0.006234079977346852 7.44050466179505E-5 1.594393856098939E-4 56-57 0.006653937026119573 7.44050466179505E-5 1.594393856098939E-4 58-59 0.007265121337624166 7.44050466179505E-5 1.6475403179689037E-4 60-61 0.008110150081356604 8.503433899194343E-5 1.7006867798388683E-4 62-63 0.008689446515739219 1.0629292373992927E-4 1.7006867798388683E-4 64-65 0.009523845967097664 1.169222161139222E-4 1.8069797035787978E-4 66-67 0.010161603509537239 1.2223686230091867E-4 1.8069797035787978E-4 68-69 0.010799361051976814 1.2755150848791513E-4 1.8069797035787978E-4 70-71 0.01143711859441639 1.2755150848791513E-4 1.8069797035787978E-4 72-73 0.012218371583904871 1.2755150848791513E-4 2.4447372460183734E-4 74-75 0.013387593745044092 1.2755150848791513E-4 2.4447372460183734E-4 76-77 0.014190105319280558 1.2755150848791513E-4 2.4447372460183734E-4 78-79 0.015354012834232784 4.251716949597171E-4 2.4447372460183734E-4 80-81 0.01642757136400607 4.251716949597171E-4 2.4447372460183734E-4 82-83 0.017485185955218363 4.304863411467136E-4 2.4447372460183734E-4 84-85 0.019276221720236172 4.3580098733371004E-4 2.4447372460183734E-4 86-87 0.02065271508266826 4.3580098733371004E-4 2.4447372460183734E-4 88-89 0.02279983214221483 4.3580098733371004E-4 2.497883707888338E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATCG 150 2.5710027E-5 14.250147 32-33 CGATCTA 430 0.0 13.808136 34-35 TCCGATC 875 0.0 13.028707 32-33 CCGATCT 870 0.0 12.01162 32-33 CTTCCGA 1180 0.0 11.874807 30-31 GACCGGA 415 4.180025E-5 11.445963 9 GACGTGT 1185 0.0 10.422021 20-21 TTCCGAT 1205 0.0 9.854611 30-31 CCGTTAA 435 8.1904E-4 9.825024 4 TCAGACG 1690 0.0 9.134783 18-19 GACGTTG 450 4.2291504E-8 8.972291 20-21 CGTGATG 2990 0.0 8.893954 1 TCCGACG 215 0.008188655 8.837372 18-19 CAGACGT 1490 0.0 8.448142 18-19 CCGACGT 310 0.0023360294 7.661431 18-19 GTGACTG 2755 0.0 7.5843496 7 TAAGACG 815 7.023118E-9 6.702577 18-19 TCCGATA 390 0.002908414 6.6987877 32-33 TGACTGG 3825 0.0 6.3328605 8 AGACGTG 1700 0.0 6.286813 20-21 >>END_MODULE