##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n02 6-9147.34200000001b43.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12714643 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.42589477345137 38.0 32.0 38.0 32.0 38.0 2 35.47591788459967 38.0 32.0 38.0 32.0 38.0 3 35.35211141988021 38.0 32.0 38.0 32.0 38.0 4 35.31161669265901 38.0 32.0 38.0 32.0 38.0 5 35.12246973823803 38.0 32.0 38.0 32.0 38.0 6 37.447732979997944 40.0 38.0 40.0 32.0 40.0 7 37.561279384722006 40.0 38.0 40.0 32.0 40.0 8 37.65769797862197 40.0 38.0 40.0 32.0 40.0 9 37.69670033205022 40.0 38.0 40.0 32.0 40.0 10-11 37.753437945524695 40.0 38.0 40.0 32.0 40.0 12-13 37.73793412839039 40.0 38.0 40.0 32.0 40.0 14-15 37.70836786373003 40.0 38.0 40.0 32.0 40.0 16-17 37.69733912308824 40.0 38.0 40.0 32.0 40.0 18-19 37.680490006679705 40.0 38.0 40.0 32.0 40.0 20-21 37.67239221738274 40.0 38.0 40.0 32.0 40.0 22-23 37.66544530585719 40.0 38.0 40.0 32.0 40.0 24-25 37.61047781679753 40.0 38.0 40.0 32.0 40.0 26-27 37.59266709257979 40.0 38.0 40.0 32.0 40.0 28-29 37.52527278980621 40.0 38.0 40.0 32.0 40.0 30-31 37.4900543019572 40.0 38.0 40.0 32.0 40.0 32-33 37.49634236682854 40.0 38.0 40.0 32.0 40.0 34-35 37.42852312094016 40.0 38.0 40.0 32.0 40.0 36-37 37.43144569611589 40.0 38.0 40.0 32.0 40.0 38-39 37.38413036842638 40.0 38.0 40.0 32.0 40.0 40-41 37.3080404223697 40.0 38.0 40.0 32.0 40.0 42-43 37.12671680990178 40.0 38.0 40.0 32.0 40.0 44-45 37.042058318114 40.0 38.0 40.0 32.0 40.0 46-47 36.965428915306546 38.0 38.0 40.0 32.0 40.0 48-49 36.88957114250081 38.0 38.0 40.0 32.0 40.0 50-51 36.82481273756565 38.0 38.0 40.0 32.0 40.0 52-53 36.754175913551016 38.0 38.0 40.0 32.0 40.0 54-55 36.70565241194739 38.0 38.0 40.0 32.0 40.0 56-57 36.6830409237601 38.0 38.0 40.0 32.0 40.0 58-59 36.612916737025174 38.0 38.0 40.0 32.0 40.0 60-61 36.575684704635435 38.0 38.0 40.0 32.0 40.0 62-63 36.49855583833538 38.0 38.0 40.0 32.0 40.0 64-65 36.483682711343135 38.0 38.0 40.0 32.0 40.0 66-67 36.419616579089165 38.0 38.0 40.0 27.0 40.0 68-69 36.368221270546094 38.0 38.0 40.0 27.0 40.0 70-71 36.25441748541426 38.0 38.0 40.0 27.0 40.0 72-73 36.18675486995585 38.0 38.0 40.0 27.0 40.0 74-75 36.098788105965696 38.0 38.0 40.0 27.0 40.0 76-77 36.014466155282534 38.0 38.0 40.0 27.0 40.0 78-79 35.92969944181681 38.0 38.0 40.0 27.0 40.0 80-81 35.85217937302683 38.0 38.0 40.0 27.0 40.0 82-83 35.764235220760824 38.0 38.0 40.0 27.0 40.0 84-85 35.65948308576183 38.0 38.0 40.0 27.0 40.0 86-87 35.59793464118497 38.0 38.0 40.0 27.0 40.0 88-89 35.52978184287203 38.0 38.0 40.0 27.0 40.0 90-91 35.4064374438197 38.0 38.0 40.0 27.0 40.0 92-93 35.3006739552184 38.0 38.0 40.0 27.0 40.0 94-95 35.20254198250002 38.0 38.0 40.0 27.0 40.0 96-97 35.121493973523286 38.0 38.0 40.0 27.0 40.0 98-99 34.983706699433085 38.0 38.0 40.0 27.0 40.0 100-101 33.03907537946602 38.0 29.5 39.0 20.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.15599432672618008 2201 2 0.27106506103607586 2201 3 0.42096777887870473 2201 4 0.3474366121930501 2201 5 0.26842862056962247 2201 6 0.2692422401732344 2201 7 0.2660394616432953 2201 8 0.1808901135192471 2201 9 0.17656819215249442 2201 10-11 0.1540183890703659 2201 12-13 0.2288250562034051 2201 14-15 0.2125249119877921 2201 16-17 0.1902563893366107 2201 18-19 0.1963622058949852 2201 20-21 0.26123249287909545 2201 22-23 0.2148734541486803 2201 24-25 0.26070546858865384 2201 26-27 0.23731936500982442 2201 28-29 0.26992783763524386 2201 30-31 0.2966350624744152 2201 32-33 0.250364574461571 2201 34-35 0.26632058662283953 2201 36-37 0.12751437334479476 2201 38-39 0.20729403885137998 2201 40-41 0.20179542486354762 2201 42-43 0.21695895953310185 2201 44-45 0.2469976612998721 2201 46-47 0.23970212864439588 2201 48-49 0.133284415828264 2201 50-51 0.2473314683306569 2201 52-53 0.20361005107979935 2201 54-55 0.27877657444268067 2201 56-57 0.10837191941163127 2201 58-59 0.18931573003371227 2201 60-61 0.09931291832375422 2201 62-63 0.18496931576349596 2201 64-65 0.132372781946259 2201 66-67 0.09554883272433301 2201 68-69 0.11835110331642795 2201 70-71 0.2307437702719497 2201 72-73 0.05535056466593602 2201 74-75 0.11938642250008513 2201 76-77 0.07856088962368801 2201 78-79 0.15349328730098222 2201 80-81 0.2617709682995297 2201 82-83 0.1388058710005282 2201 84-85 0.12357859192069753 2201 86-87 0.14701846580249622 2201 88-89 0.1174608761355529 2201 90-91 0.05184320900058026 2201 92-93 -0.032621311056637126 2201 94-95 0.12768836136767447 2201 96-97 -0.07617060392784225 2201 98-99 0.024574744715586405 2201 100-101 0.2230256429800761 1101 1 0.21847704448244798 1101 2 0.16931993369850318 1101 3 0.22801831888620683 1101 4 0.15873824065987208 1101 5 0.17668731464743104 1101 6 0.23322962234625777 1101 7 0.25939052806150187 1101 8 0.23385026488737282 1101 9 0.22398142312341918 1101 10-11 0.2627368981744951 1101 12-13 0.25309091507725157 1101 14-15 0.23000951898313104 1101 16-17 0.22270550153737645 1101 18-19 0.2582217395391879 1101 20-21 0.28181727677590374 1101 22-23 0.23268202270659089 1101 24-25 0.2496671599728657 1101 26-27 0.26471605798271725 1101 28-29 0.2834846736750549 1101 30-31 0.2991587709934507 1101 32-33 0.24831808486813145 1101 34-35 0.2873380492030151 1101 36-37 0.2294038628781152 1101 38-39 0.2599346941310827 1101 40-41 0.24351617099848255 1101 42-43 0.27147059839073506 1101 44-45 0.24478921239376916 1101 46-47 0.24387223744739117 1101 48-49 0.21579031970034634 1101 50-51 0.2790707132647796 1101 52-53 0.29349626020711383 1101 54-55 0.22893317375511657 1101 56-57 0.2375994138512567 1101 58-59 0.18106109352617494 1101 60-61 0.17139096679704124 1101 62-63 0.19188388780791854 1101 64-65 0.2160457489389529 1101 66-67 0.20637899585726416 1101 68-69 0.2134590332542814 1101 70-71 0.24313245544743722 1101 72-73 0.2828982607961521 1101 74-75 0.36259887315802075 1101 76-77 0.31860253160142804 1101 78-79 0.24283774996990104 1101 80-81 0.3397107674104376 1101 82-83 0.32685421548323035 1101 84-85 0.33001269612081785 1101 86-87 0.30107886277169627 1101 88-89 0.27013309029511845 1101 90-91 0.2250866746665139 1101 92-93 0.2671099518424285 1101 94-95 0.2649317611964932 1101 96-97 0.2155123288009264 1101 98-99 0.2982935615366884 1101 100-101 0.3537388951333824 2202 1 0.05581366372679497 2202 2 0.028809715306707062 2202 3 0.11019010006003072 2202 4 0.04321970244633633 2202 5 -0.02320176435389243 2202 6 0.024939642332313383 2202 7 0.16875139012216778 2202 8 0.17937672268472227 2202 9 0.188696795459812 2202 10-11 0.18591006595980986 2202 12-13 0.16572107351436216 2202 14-15 0.1654135611513894 2202 16-17 0.11082782062584329 2202 18-19 0.17261257317497325 2202 20-21 0.1665166618962317 2202 22-23 0.17615382564930115 2202 24-25 0.17618654019363333 2202 26-27 0.16778514473202932 2202 28-29 0.14296412454556418 2202 30-31 0.14205274701455295 2202 32-33 0.20956951986456573 2202 34-35 0.1539434008224987 2202 36-37 0.1662415641126529 2202 38-39 0.14132273566095677 2202 40-41 0.1432579126296858 2202 42-43 0.1205134388692386 2202 44-45 0.09990930906057116 2202 46-47 0.012524547626128424 2202 48-49 0.06217933816924415 2202 50-51 0.13026312007946927 2202 52-53 0.14499595436308965 2202 54-55 0.10792765387592596 2202 56-57 0.06170348357694877 2202 58-59 0.08618276481416132 2202 60-61 0.016957647776685292 2202 62-63 0.09148083584816646 2202 64-65 0.018504818132399237 2202 66-67 0.03472702746128675 2202 68-69 -0.0012598370823937444 2202 70-71 0.009037593556207923 2202 72-73 -0.023626941945103397 2202 74-75 0.028774444205012628 2202 76-77 -0.06486750962745447 2202 78-79 -0.0076490573862670885 2202 80-81 0.041512903465189765 2202 82-83 -0.01792386095730336 2202 84-85 0.05930837108765985 2202 86-87 0.04806621783759368 2202 88-89 0.008466448222137046 2202 90-91 0.08591925938610245 2202 92-93 0.0386904703693105 2202 94-95 0.12826025770205973 2202 96-97 -0.016363941169437624 2202 98-99 -0.03380396164151023 2202 100-101 0.12857839319375586 1102 1 -0.050742935601256534 1102 2 -0.0721818428455947 1102 3 -0.12516504008026175 1102 4 -0.1855325637172598 1102 5 -0.1373586143042047 1102 6 -0.1696627689419259 1102 7 0.004581723806779792 1102 8 0.008636517514986508 1102 9 0.09958486594710081 1102 10-11 0.014936734988637568 1102 12-13 0.1206853260511096 1102 14-15 0.07066117119452286 1102 16-17 0.0828966417005148 1102 18-19 0.054553771741446155 1102 20-21 0.14306033642072435 1102 22-23 0.09332358799810692 1102 24-25 0.09932694156725574 1102 26-27 0.0798653628874888 1102 28-29 -0.03646411618966994 1102 30-31 0.059637259460863845 1102 32-33 0.06864917734702658 1102 34-35 0.06719715735651732 1102 36-37 0.08010607956396143 1102 38-39 0.09285159480337057 1102 40-41 0.11391079874543664 1102 42-43 -0.012805635356968992 1102 44-45 -0.017128731351633064 1102 46-47 0.008923433343184684 1102 48-49 -0.06857432610571124 1102 50-51 -0.04923106764334051 1102 52-53 -0.034267486634902866 1102 54-55 -0.03722107822135712 1102 56-57 -0.08683005648082087 1102 58-59 -0.0740821425382805 1102 60-61 -0.02625682256580575 1102 62-63 -0.090320370400228 1102 64-65 -0.12683977038827976 1102 66-67 -0.18584790367984994 1102 68-69 -0.2510100264187969 1102 70-71 -0.11835690598685034 1102 72-73 -0.06205761923069275 1102 74-75 -0.034889510347056785 1102 76-77 -0.12269025369532471 1102 78-79 -0.12005322720687417 1102 80-81 -0.06475304889961819 1102 82-83 -0.08205859328431586 1102 84-85 -0.02231072014836144 1102 86-87 -0.10088803948206504 1102 88-89 -0.20032640827336934 1102 90-91 -0.28398098882727396 1102 92-93 -0.13367315233221433 1102 94-95 -0.15272253230836697 1102 96-97 -0.13609963093983168 1102 98-99 -0.26251440346901944 1102 100-101 -0.11468369864259387 2203 1 -0.08749742748572231 2203 2 -0.10163468767817108 2203 3 -0.18722320519272984 2203 4 -0.18790745333719627 2203 5 -0.214873089305037 2203 6 -0.15570128607929945 2203 7 -0.0059791798681203545 2203 8 -0.031865529370719514 2203 9 0.009704351496083063 2203 10-11 0.02624522793423978 2203 12-13 0.05575954251654025 2203 14-15 0.05826517000762266 2203 16-17 0.083480471094866 2203 18-19 0.04591242716720245 2203 20-21 0.07306637174583841 2203 22-23 -0.038141760989738316 2203 24-25 -0.06557176265908993 2203 26-27 -0.03754864787322276 2203 28-29 -0.03701872998904321 2203 30-31 -0.052620256821320766 2203 32-33 -0.08468401297027839 2203 34-35 -0.01833609646043044 2203 36-37 -0.05971478035625921 2203 38-39 -0.0787084822857409 2203 40-41 -0.014564012427477735 2203 42-43 -0.08442104565544639 2203 44-45 -0.13923069273015187 2203 46-47 -0.17062730959878536 2203 48-49 -0.20687095769883257 2203 50-51 -0.14901682945330919 2203 52-53 -0.11955184745927028 2203 54-55 -0.1692876317579035 2203 56-57 -0.36614415542487677 2203 58-59 -0.17155492128073746 2203 60-61 -0.24378458910373269 2203 62-63 -0.22345953421434928 2203 64-65 -0.2526407344949533 2203 66-67 -0.27869956620870084 2203 68-69 -0.1392333106192325 2203 70-71 -0.1758749046338295 2203 72-73 -0.2280078328457691 2203 74-75 -0.1400852840489364 2203 76-77 -0.20210715306590998 2203 78-79 -0.30563192503617387 2203 80-81 -0.2633890831995629 2203 82-83 -0.30993963214364584 2203 84-85 -0.318787012979719 2203 86-87 -0.3778457315665307 2203 88-89 -0.38293158363595126 2203 90-91 -0.28181019053273104 2203 92-93 -0.35898943502427727 2203 94-95 -0.25592672433305097 2203 96-97 -0.30882396275314505 2203 98-99 -0.3854020620648484 2203 100-101 -0.19347577559078388 1103 1 -0.016951761824408607 1103 2 -0.03901570988305991 1103 3 -0.11019754921089486 1103 4 -0.13742906048272374 1103 5 -0.0867924961080675 1103 6 -0.07253302143048757 1103 7 0.09717791161168776 1103 8 0.03216131875818462 1103 9 0.08319124099073605 1103 10-11 0.13553492127981315 1103 12-13 0.10712466365627193 1103 14-15 0.14400602636069237 1103 16-17 0.11738878689833143 1103 18-19 0.08816929325920597 1103 20-21 0.08629314184417325 1103 22-23 0.10497510140780975 1103 24-25 0.1481287060733436 1103 26-27 0.04923452107279047 1103 28-29 0.04400688395821106 1103 30-31 0.09504945316039226 1103 32-33 0.07957795810062152 1103 34-35 0.1343756814423145 1103 36-37 0.10088620700553008 1103 38-39 0.12414056910908045 1103 40-41 0.07211556926601048 1103 42-43 0.012099320279446601 1103 44-45 -0.08565307433465819 1103 46-47 -0.056219461181484576 1103 48-49 -0.019539155784450202 1103 50-51 -0.04966848703900695 1103 52-53 -0.030008178681178777 1103 54-55 -0.0138385718282521 1103 56-57 0.025668048695735024 1103 58-59 -0.03657889007885018 1103 60-61 -0.09555912250168319 1103 62-63 -0.09380457562224365 1103 64-65 -0.0619547354545773 1103 66-67 -0.15593873639440403 1103 68-69 -0.0919163656305102 1103 70-71 -0.04925416141166039 1103 72-73 -0.0448454491121808 1103 74-75 -0.10598431238838657 1103 76-77 -0.1911507600934499 1103 78-79 -0.16477891579236825 1103 80-81 -0.14643666402109545 1103 82-83 -0.24652879991068488 1103 84-85 -0.1663757652325799 1103 86-87 -0.28524551298543344 1103 88-89 -0.20348399807016904 1103 90-91 -0.14654594889157124 1103 92-93 -0.16678236895551635 1103 94-95 -0.11102740066869643 1103 96-97 -0.21040452251811104 1103 98-99 -0.10714016101781709 1103 100-101 -0.08350807357464873 2204 1 -0.09111401570493172 2204 2 -0.023256121061592694 2204 3 0.01650250629111838 2204 4 -0.11722167017317986 2204 5 -0.06993998865128503 2204 6 -0.017498356510351698 2204 7 0.06777220619734692 2204 8 0.10078301412459467 2204 9 0.08533161218533536 2204 10-11 0.10754862563859291 2204 12-13 0.1558635968551343 2204 14-15 0.11198277592161077 2204 16-17 0.1333706782558508 2204 18-19 0.14373128924364664 2204 20-21 0.11696689198901566 2204 22-23 0.07073344494003919 2204 24-25 0.09656778762771978 2204 26-27 0.14228930322322242 2204 28-29 0.1494243491767051 2204 30-31 0.10840595246504137 2204 32-33 0.10803304633542865 2204 34-35 0.050681039150710205 2204 36-37 0.14556104851575924 2204 38-39 0.1681661274004469 2204 40-41 0.012971930170259327 2204 42-43 -0.02424159128987924 2204 44-45 -0.032622306065533735 2204 46-47 0.03897332189417568 2204 48-49 -0.04447593378715453 2204 50-51 -0.007557617078866485 2204 52-53 0.042777415568608035 2204 54-55 -0.056142677100673666 2204 56-57 0.0352265774070446 2204 58-59 0.08417431670257258 2204 60-61 0.03269209922402894 2204 62-63 0.03452020525847388 2204 64-65 0.03212600073241134 2204 66-67 0.03648970566722198 2204 68-69 -0.001817704425093325 2204 70-71 0.04974139115496712 2204 72-73 0.04566308463865454 2204 74-75 0.02184813032521049 2204 76-77 0.029701382090465245 2204 78-79 0.004314350051494387 2204 80-81 0.0471673508417112 2204 82-83 0.03608090814162068 2204 84-85 0.09507083566018792 2204 86-87 0.03960911094232955 2204 88-89 0.08219159504888296 2204 90-91 0.089623143287902 2204 92-93 0.12414994741016017 2204 94-95 0.1272528877420882 2204 96-97 0.020948756919246136 2204 98-99 -0.0027558279811756847 2204 100-101 0.11873452266901552 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42-43 0.07417359363543596 1215 44-45 0.1361852970715276 1215 46-47 0.11671659539008772 1215 48-49 0.09159393860181808 1215 50-51 0.08863600118063175 1215 52-53 0.056127131645517636 1215 54-55 0.08361978503720024 1215 56-57 0.08669412658791487 1215 58-59 0.15373551345702197 1215 60-61 0.09787892550406241 1215 62-63 0.03808050446262712 1215 64-65 -0.047072330486216174 1215 66-67 0.066165133919597 1215 68-69 0.0739086174388035 1215 70-71 0.10489998289660463 1215 72-73 0.18104464902769024 1215 74-75 0.12834316552962832 1215 76-77 0.16058256480322086 1215 78-79 0.1715447391225311 1215 80-81 0.20982590627568953 1215 82-83 0.28908248171538986 1215 84-85 0.20774287523204293 1215 86-87 0.23444322429210018 1215 88-89 0.22642308982781856 1215 90-91 0.16939408191883132 1215 92-93 0.19620409096543057 1215 94-95 0.2369727445623937 1215 96-97 0.16824701457753122 1215 98-99 0.20129257260249034 1215 100-101 0.06983493864949963 1212 1 0.1550451458840243 1212 2 -0.0017918021818061902 1212 3 0.10980305542712188 1212 4 -0.005861227325127061 1212 5 0.176495750289277 1212 6 -0.10318510639833534 1212 7 -0.15513576216996938 1212 8 -0.2715442592202564 1212 9 -0.34136315881626444 1212 10-11 -0.26768072501023 1212 12-13 -0.3519141303685771 1212 14-15 -0.31675914483341927 1212 16-17 -0.2408606546948988 1212 18-19 -0.21151169772465295 1212 20-21 -0.19032098377768847 1212 22-23 -0.2015291867570923 1212 24-25 -0.1257397311904498 1212 26-27 -0.12504116166756774 1212 28-29 -0.11415382147374942 1212 30-31 -0.1179113089376358 1212 32-33 -0.10949670409357992 1212 34-35 -0.12719942628872616 1212 36-37 -0.09179297224156358 1212 38-39 -0.06726302737349243 1212 40-41 -0.08478776392777121 1212 42-43 0.08207988869843774 1212 44-45 0.057486952227293386 1212 46-47 0.04477625341981195 1212 48-49 0.13784557849053414 1212 50-51 -0.08868495371647356 1212 52-53 0.004973821202248985 1212 54-55 0.05787721846487415 1212 56-57 0.12774110046120768 1212 58-59 0.013274321014812074 1212 60-61 0.03044973155472519 1212 62-63 0.1122857603669587 1212 64-65 0.0724692556396036 1212 66-67 0.11510382480368975 1212 68-69 0.08325049429545572 1212 70-71 0.09822901143025575 1212 72-73 0.0432516590369616 1212 74-75 0.09147469813328257 1212 76-77 0.15738103139153026 1212 78-79 0.14047046651492678 1212 80-81 0.052770032341364015 1212 82-83 0.07326287266761966 1212 84-85 0.02563077742190245 1212 86-87 -0.020883516562086868 1212 88-89 -0.03244535952367755 1212 90-91 -0.0026836654436195317 1212 92-93 0.0382211355535631 1212 94-95 -0.013600395985818636 1212 96-97 -0.06763802947250497 1212 98-99 0.012044617751953979 1212 100-101 -0.17553503614701782 1213 1 -0.1228771506122186 1213 2 0.0012087476996569535 1213 3 0.10672264273829768 1213 4 0.18031290721987148 1213 5 0.2880505730242433 1213 6 0.16962282961522845 1213 7 0.03122738326550234 1213 8 -0.19767750788113858 1213 9 -0.18611585208445547 1213 10-11 -0.22029737406458594 1213 12-13 -0.19440635965286646 1213 14-15 -0.14145249094848822 1213 16-17 -0.13644012806643957 1213 18-19 -0.11319313596031577 1213 20-21 -0.1780382260606146 1213 22-23 -0.2271548006208448 1213 24-25 -0.3370592014314582 1213 26-27 -0.2287120442931183 1213 28-29 -0.21239377781947866 1213 30-31 -0.44104403697927097 1213 32-33 -0.313433366602176 1213 34-35 -0.4255761498202162 1213 36-37 -0.45444419274750203 1213 38-39 -0.5907686784758184 1213 40-41 -0.38746217749247336 1213 42-43 -0.10713666641886732 1213 44-45 -0.21433164140626104 1213 46-47 -0.2831387585569587 1213 48-49 -0.18729919767636005 1213 50-51 -0.3780004080457928 1213 52-53 -0.3563869850290402 1213 54-55 -0.11756066672571563 1213 56-57 -0.19213593862343714 1213 58-59 -0.33373142314137993 1213 60-61 -0.2144199263564346 1213 62-63 -0.44274638302970004 1213 64-65 -0.44945950848132554 1213 66-67 -0.21157353179555827 1213 68-69 -0.23648979977177476 1213 70-71 -0.25623600973484173 1213 72-73 -0.18121463796095583 1213 74-75 -0.2685513943681812 1213 76-77 -0.2274256095293481 1213 78-79 -0.28430275925544635 1213 80-81 -0.18394622385793014 1213 82-83 -0.0897046768774814 1213 84-85 -0.07487650090818221 1213 86-87 -0.15996000850349645 1213 88-89 -0.13143739041010605 1213 90-91 -0.16272641612925298 1213 92-93 -0.24441153607406818 1213 94-95 -0.322928773398516 1213 96-97 -0.33978762847899446 1213 98-99 -0.2232499627180644 1213 100-101 -0.9300468298682958 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72.0 3 53.0 4 102.0 5 112.0 6 114.0 7 82.0 8 54.0 9 61.0 10 71.0 11 78.0 12 96.0 13 132.0 14 687.0 15 6253.0 16 28868.0 17 55546.0 18 61772.0 19 57885.0 20 58823.0 21 63849.0 22 72417.0 23 81311.0 24 90939.0 25 101702.0 26 113113.0 27 127422.0 28 143775.0 29 164117.0 30 188872.0 31 218579.0 32 258576.0 33 311934.0 34 385894.0 35 507104.0 36 725246.0 37 1233155.0 38 2976112.0 39 4679665.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.12673970784525 30.755473074458006 29.285009638160588 19.83277757953616 2 21.352775465909783 31.912395731956984 28.070186477168818 18.664642324964415 3 21.17000568880245 29.68221750195899 28.777821393939856 20.3699554152987 4 21.260204871754397 29.376526272233367 28.85218350573244 20.5110853502798 5 21.425483603130022 29.879300184590836 28.871845445841764 19.823370766437378 6 22.198307699205607 29.324062937794533 27.846269174466503 20.63136018853335 7 21.996622160818088 29.912605824920135 28.450330732004385 19.64044128225739 8 20.862068490861002 29.820287689146273 29.146398969589114 20.17124485040361 9 21.156741439679504 29.280883842463396 29.25264792357457 20.309726794282533 10-11 21.28633584113818 29.620595002698437 28.57386966795205 20.51919948821133 12-13 21.03583643599888 29.632520748805458 29.112402466023273 20.219240349172384 14-15 21.233780201440762 29.35940302612744 28.939080448375552 20.467736324056247 16-17 21.351875001573227 29.345347059830395 28.95158445829442 20.35119348030196 18-19 20.98830070259977 29.267867016987143 29.023660635021553 20.720171645391535 20-21 21.346831977864113 29.205741250414214 28.707172433187704 20.74025433853397 22-23 21.328350498918077 29.131620089650422 28.762895430497004 20.777133980934494 24-25 21.53819787138977 28.87040666294739 28.712613388225382 20.878782077437457 26-27 21.73503204976418 29.040928448022363 28.464290720884872 20.759748781328582 28-29 21.471318938265362 29.037582354894358 28.589871318135796 20.90122738870448 30-31 21.746970816132354 28.683277400770102 28.568646821414802 21.001104961682742 32-33 21.681424110436488 29.03147140050435 28.464561009616396 20.822543479442768 34-35 21.880832470254255 28.771696219029252 28.330043065587663 21.017428245128826 36-37 21.79621633671259 29.02426418574778 28.29066751223779 20.888851965301846 38-39 21.68648679947607 28.82667525352459 28.501222219014128 20.985615727985206 40-41 21.97397340883626 28.933907834707217 28.185007348845154 20.907111407611367 42-43 21.813082348809026 28.952949607388856 28.320333368949672 20.913634674852446 44-45 21.866609173401354 28.630351975532477 28.295454491814766 21.207584359251406 46-47 21.951238508649183 28.923045466306945 28.126509276669854 20.99920674837402 48-49 21.72206321588498 28.73779255150202 28.37493556654145 21.165208666071546 50-51 21.91978454793934 28.895387261389043 28.0704829732762 21.114345217395407 52-53 21.846534390185724 28.908719818940394 28.31135307567836 20.933392715195527 54-55 22.000756369843298 28.613056561028483 28.225674673010083 21.160512396118143 56-57 22.009762510761345 28.911222057136616 28.07432022745548 21.004695204646566 58-59 21.80883309931797 28.805170092773295 28.2706977727737 21.11529903513503 60-61 21.908054454114676 28.730365148271442 27.988891905672936 21.37268849194095 62-63 21.80834540406671 28.828160498119697 28.21719381971633 21.146300278097264 64-65 21.96394938263189 28.62235885350199 28.146972840350397 21.26671892351573 66-67 21.997302441608955 28.728752990895252 27.9733737062399 21.300570861255892 68-69 21.67663259769074 28.700028655780358 28.222903029193297 21.400435717335604 70-71 22.082871079357574 28.683486122507247 27.86907732237951 21.36456547575567 72-73 21.91706923469922 28.861404676732548 28.009726385130847 21.211799703437386 74-75 22.021509066433445 28.57962926750824 28.04855650524895 21.35030516080937 76-77 22.016022511498445 28.902104587659565 27.91812344070907 21.16374946013292 78-79 21.97302911150701 28.716581282548148 28.0874286586707 21.222960947274146 80-81 22.139070929869934 28.63133940578535 27.885102192568496 21.344487471776223 82-83 21.95435322377033 28.966585192596582 28.044737982600893 21.034323601032202 84-85 21.97184647959423 28.705308600751806 27.980278080822995 21.342566838830972 86-87 21.898517266090188 28.86393818740735 27.947320301008418 21.290224245494045 88-89 21.92876427879544 28.729537033164455 28.00322173778295 21.338476950257153 90-91 22.115508060356664 28.59011126887149 27.835056900558197 21.459323770213647 92-93 21.83774846683602 28.82877083898265 28.02812771826075 21.305352975920584 94-95 22.001079720405848 28.63184646393141 27.90259648717479 21.464477328487956 96-97 21.998462678039093 28.75845749382683 27.875359040930174 21.367720787203904 98-99 21.837975324640883 28.802182023011795 27.934689297956343 21.42515335439098 100-101 22.346997753119705 28.516315723845615 27.411872718046016 21.72481380498867 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 152.0 1 210.5 2 281.5 3 306.5 4 321.5 5 378.0 6 437.0 7 485.5 8 568.0 9 660.0 10 769.5 11 957.0 12 1229.0 13 1671.0 14 2144.5 15 2802.0 16 3781.5 17 5386.5 18 7739.0 19 10613.0 20 14713.0 21 20092.5 22 26276.0 23 33665.0 24 42868.5 25 53505.5 26 64579.5 27 76905.0 28 92482.5 29 111298.0 30 132599.5 31 161272.0 32 202715.0 33 257475.5 34 324680.5 35 408710.0 36 507534.5 37 608186.5 38 689561.0 39 737407.5 40 752367.0 41 724886.0 42 664717.0 43 589397.5 44 513387.5 45 451874.0 46 403652.0 47 374785.5 48 359490.5 49 345395.5 50 329670.0 51 309525.0 52 285078.5 53 259474.0 54 237285.5 55 218832.0 56 199780.0 57 181599.5 58 165089.5 59 145226.0 60 123742.0 61 102066.0 62 82275.0 63 66122.0 64 52647.0 65 41488.0 66 31532.5 67 23899.5 68 18381.5 69 13662.0 70 9983.0 71 7452.0 72 5596.5 73 4187.5 74 3109.5 75 2253.0 76 1754.5 77 1442.5 78 1089.5 79 799.5 80 648.5 81 449.0 82 305.0 83 223.0 84 147.5 85 108.0 86 86.0 87 67.5 88 42.0 89 27.0 90 23.0 91 18.5 92 15.5 93 15.0 94 15.0 95 9.0 96 9.0 97 9.5 98 11.0 99 14.5 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.291958020370686E-4 2 4.4830200895141137E-4 3 0.0015100699248889646 4 0.0019662368813658394 5 0.008800876280993497 6 0.013944551962646535 7 0.014015336490375705 8 0.009972753462287537 9 0.0026819471061830047 10-11 0.006303755441658881 12-13 0.010861492532664897 14-15 0.03464509384966609 16-17 0.014998454931058622 18-19 0.02244656023767242 20-21 0.017739389143682602 22-23 0.030980028302800163 24-25 0.026673969532608977 26-27 0.022407235500045107 28-29 0.030496334029984168 30-31 0.03340636461440561 32-33 0.03764163885686763 34-35 0.018915198798739374 36-37 0.029159292950655398 38-39 0.029462093430385736 40-41 0.03258054512423196 42-43 0.04027639627789786 44-45 0.02822729666888799 46-47 0.02886435741845052 48-49 0.022694306084724516 50-51 0.0363596524102171 52-53 0.02902952131648525 54-55 0.030909243775070993 56-57 0.02403921211157875 58-59 0.025380185664670252 60-61 0.033599055828779464 62-63 0.02990646296557442 64-65 0.029175022845706323 66-67 0.02475885481015865 68-69 0.026902053010847412 70-71 0.030103086653711 72-73 0.03680402194540578 74-75 0.031849105004363866 76-77 0.03412207483922278 78-79 0.03564000971163721 80-81 0.0415229904606838 82-83 0.04315496707221744 84-85 0.04831830512268414 86-87 0.04950197972526637 88-89 0.05186539645666811 90-91 0.051527203713073184 92-93 0.05197943819578733 94-95 0.05287997468745288 96-97 0.05371759159891473 98-99 0.05494452341288701 100-101 0.05873936059392308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2714643E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.424977890803515 #Duplication Level Percentage of deduplicated Percentage of total 1 44.89030402201995 6.924319470511059 2 2.2976269167306214 0.7088168878376977 3 2.17738736706345 1.007584559900058 4 3.5494566879643115 2.190011637448567 5 3.857219526598965 2.9748762958882313 6 4.184011211896296 3.8722968263024633 7 4.890899558588462 5.280941229015738 8 3.866935485276838 4.771791549244703 9 4.003941157168459 5.558463344286154 >10 25.79925784213185 60.84316890380227 >50 0.39497848525909107 4.012229187733995 >100 0.08733959439620574 1.7528154034612342 >500 3.565914249212037E-4 0.04877837445665612 >1k 2.855534805274677E-4 0.053906330111167376 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.5729895050926714E-5 0.0 2.3594842576390072E-5 2 1.5729895050926714E-5 0.0 2.3594842576390072E-5 3 1.5729895050926714E-5 1.1797421288195037E-4 2.3594842576390072E-5 4 1.5729895050926714E-5 1.1797421288195037E-4 2.3594842576390072E-5 5 1.5729895050926714E-5 1.1797421288195037E-4 2.3594842576390072E-5 6 1.5729895050926714E-5 1.1797421288195037E-4 2.3594842576390072E-5 7 1.5729895050926714E-5 1.1797421288195037E-4 2.3594842576390072E-5 8 2.3594842576390072E-5 1.1797421288195037E-4 2.3594842576390072E-5 9 2.3594842576390072E-5 1.1797421288195037E-4 2.3594842576390072E-5 10-11 3.5392263864585105E-5 1.2190668664468204E-4 2.3594842576390072E-5 12-13 5.8987106440975184E-5 1.258391604074137E-4 2.3594842576390072E-5 14-15 9.831184406829197E-5 1.258391604074137E-4 2.3594842576390072E-5 16-17 1.4156905545834042E-4 1.258391604074137E-4 2.3594842576390072E-5 18-19 3.224628485439977E-4 1.258391604074137E-4 2.752731633912175E-5 20-21 5.1515406291785E-4 1.258391604074137E-4 6.291958020370686E-5 22-23 6.921153822407755E-4 1.258391604074137E-4 6.291958020370686E-5 24-25 9.477261768183346E-4 1.258391604074137E-4 6.291958020370686E-5 26-27 0.0012151343926840887 1.258391604074137E-4 6.291958020370686E-5 28-29 0.0014982725036007697 1.258391604074137E-4 6.291958020370686E-5 30-31 0.0018403977209584257 1.258391604074137E-4 6.685205396643854E-5 32-33 0.0021353332531633016 1.494340029838038E-4 1.10109265356487E-4 34-35 0.0026150950522165666 1.7302884556019387E-4 1.10109265356487E-4 36-37 0.002886435741845052 1.7302884556019387E-4 1.10109265356487E-4 38-39 0.0033347377507964636 1.7302884556019387E-4 1.10109265356487E-4 40-41 0.0036021459666622175 1.7302884556019387E-4 1.10109265356487E-4 42-43 0.00386562170876524 1.7302884556019387E-4 1.10109265356487E-4 44-45 0.004243139189987481 1.7302884556019387E-4 1.10109265356487E-4 46-47 0.004585264407345137 1.7302884556019387E-4 1.10109265356487E-4 48-49 0.005006039099957426 1.7302884556019387E-4 1.10109265356487E-4 50-51 0.0057492766411137135 1.7302884556019387E-4 1.10109265356487E-4 52-53 0.006087469384708638 1.7302884556019387E-4 1.10109265356487E-4 54-55 0.006622285816440147 1.7302884556019387E-4 1.10109265356487E-4 56-57 0.007046992982815168 1.7302884556019387E-4 1.10109265356487E-4 58-59 0.0076407965209876515 1.7302884556019387E-4 1.10109265356487E-4 60-61 0.008096963477464527 1.7302884556019387E-4 1.5729895050926716E-4 62-63 0.008840201018620814 1.7302884556019387E-4 1.5729895050926716E-4 64-65 0.009658155561269003 1.7302884556019387E-4 1.5729895050926716E-4 66-67 0.010204769414288706 1.7302884556019387E-4 3.4605769112038774E-4 68-69 0.011160360538632504 1.7302884556019387E-4 3.5392263864585105E-4 70-71 0.012139546505552693 1.808937930856572E-4 3.617875861713144E-4 72-73 0.012894581467997174 1.808937930856572E-4 3.617875861713144E-4 74-75 0.013504114901220585 1.808937930856572E-4 3.617875861713144E-4 76-77 0.014278812232478726 1.808937930856572E-4 4.4830200895141137E-4 78-79 0.015045644616211403 1.808937930856572E-4 5.466138530197033E-4 80-81 0.015891126475198712 1.848262668483889E-4 6.37060749562532E-4 82-83 0.01707480107778095 1.9269121437385226E-4 6.409932233252636E-4 84-85 0.01836071999819421 2.044886356620473E-4 6.449256970879953E-4 86-87 0.01971742344633664 2.044886356620473E-4 6.449256970879953E-4 88-89 0.021148843895970968 2.1235358318751063E-4 6.449256970879953E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 1295 0.0 18.157272 32-33 CCGATCT 1070 0.0 17.091972 32-33 CTTCCGA 1660 0.0 16.166828 30-31 CGATCTA 630 0.0 14.326164 34-35 TTCCGAT 1670 0.0 11.519218 30-31 GACGTGT 1455 0.0 9.957442 20-21 GACCGGA 725 1.619228E-7 9.828191 9 TCCGAAC 755 0.0 9.437555 32-33 TTCCGAA 910 0.0 8.612455 30-31 CCGAACT 890 0.0 8.539751 32-33 TGACCGG 560 8.557992E-4 8.481697 8 TCCGACC 620 2.0008883E-10 8.427838 32-33 GACGCGT 330 4.9348944E-4 7.9169827 20-21 GTGACCG 895 2.8968114E-5 7.4281993 7 TCTTCCG 3215 0.0 7.313494 28-29 TACAAGG 8825 0.0 7.264307 1 AGGTGAC 4265 0.0 6.6804748 5 AACAAGG 6910 0.0 6.5286093 1 AGGCGAC 995 8.434249E-4 6.2043366 5 ACGCGTG 425 0.0071280235 6.147304 20-21 >>END_MODULE