FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 6-9147.34200000001b43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 6-9147.34200000001b43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12714643
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC12950.018.15727232-33
CCGATCT10700.017.09197232-33
CTTCCGA16600.016.16682830-31
CGATCTA6300.014.32616434-35
TTCCGAT16700.011.51921830-31
GACGTGT14550.09.95744220-21
GACCGGA7251.619228E-79.8281919
TCCGAAC7550.09.43755532-33
TTCCGAA9100.08.61245530-31
CCGAACT8900.08.53975132-33
TGACCGG5608.557992E-48.4816978
TCCGACC6202.0008883E-108.42783832-33
GACGCGT3304.9348944E-47.916982720-21
GTGACCG8952.8968114E-57.42819937
TCTTCCG32150.07.31349428-29
TACAAGG88250.07.2643071
AGGTGAC42650.06.68047485
AACAAGG69100.06.52860931
AGGCGAC9958.434249E-46.20433665
ACGCGTG4250.00712802356.14730420-21
Produced by FastQC (version 0.11.2)