FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 5-59290.34200000001b29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 5-59290.34200000001b29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12122790
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT14350.026.15054932-33
TCCGATC14900.023.43185632-33
GATCTGG26100.019.8309611
GACCGGA4601.891749E-1015.489089
CGATCTA13550.015.24918534-35
GTGACTG24100.014.5804417
GGTGACT25550.014.1252066
TTCCGAT27750.013.52200330-31
CTGGTGA50150.012.5933744
CGGTGAC4607.093313E-712.3875335
CAGACGT14350.011.58594918-19
TGACTGG29750.011.4945278
CTTCCGA29850.011.37728530-31
CGATCTG8200.010.13726734-35
GCGACTG4409.100295E-49.71283157
TCTGGTG55500.09.6682273
TGGTGAC50200.09.6484565
TCTTCCG31300.09.56073128-29
AGACGCG3001.7022023E-59.5004220-21
GACGTGT18650.09.42400820-21
Produced by FastQC (version 0.11.2)