##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l02n02 5-59290.34200000001b29.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12122790
Sequences flagged as poor quality	0
Sequence length	101
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.43069598664994	38.0	32.0	38.0	32.0	38.0
2	35.81398324973047	38.0	38.0	38.0	32.0	38.0
3	35.77028563556739	38.0	38.0	38.0	32.0	38.0
4	35.641991241290164	38.0	32.0	38.0	32.0	38.0
5	35.60524598710363	38.0	32.0	38.0	32.0	38.0
6	37.79571344550223	40.0	38.0	40.0	38.0	40.0
7	37.91610091406351	40.0	38.0	40.0	38.0	40.0
8	38.027990998771735	40.0	38.0	40.0	38.0	40.0
9	38.03094312447877	40.0	38.0	40.0	38.0	40.0
10-11	38.056392835312664	40.0	38.0	40.0	38.0	40.0
12-13	38.012172527941175	40.0	38.0	40.0	38.0	40.0
14-15	37.97125187353736	40.0	38.0	40.0	38.0	40.0
16-17	37.95918043618672	40.0	38.0	40.0	38.0	40.0
18-19	37.94761032732564	40.0	38.0	40.0	38.0	40.0
20-21	37.93588406629167	40.0	38.0	40.0	38.0	40.0
22-23	37.91780584337434	40.0	38.0	40.0	38.0	40.0
24-25	37.857859700613474	40.0	38.0	40.0	35.0	40.0
26-27	37.84332232926579	40.0	38.0	40.0	35.0	40.0
28-29	37.775081190056085	40.0	38.0	40.0	32.0	40.0
30-31	37.74760368694005	40.0	38.0	40.0	32.0	40.0
32-33	37.753168165084105	40.0	38.0	40.0	32.0	40.0
34-35	37.674282281554	40.0	38.0	40.0	32.0	40.0
36-37	37.685801906986754	40.0	38.0	40.0	32.0	40.0
38-39	37.64232123958264	40.0	38.0	40.0	32.0	40.0
40-41	37.56942754101985	40.0	38.0	40.0	32.0	40.0
42-43	37.38077777475317	40.0	38.0	40.0	32.0	40.0
44-45	37.299732446078835	40.0	38.0	40.0	32.0	40.0
46-47	37.228700818870905	40.0	38.0	40.0	32.0	40.0
48-49	37.157682059987835	40.0	38.0	40.0	32.0	40.0
50-51	37.1017866349248	40.0	38.0	40.0	32.0	40.0
52-53	37.03546361852346	38.0	38.0	40.0	32.0	40.0
54-55	36.98810797679412	38.0	38.0	40.0	32.0	40.0
56-57	36.9696364863204	38.0	38.0	40.0	32.0	40.0
58-59	36.90857979062575	38.0	38.0	40.0	32.0	40.0
60-61	36.874407129051974	38.0	38.0	40.0	32.0	40.0
62-63	36.8028137499701	38.0	38.0	40.0	32.0	40.0
64-65	36.795181719719636	38.0	38.0	40.0	32.0	40.0
66-67	36.731234270328855	38.0	38.0	40.0	32.0	40.0
68-69	36.68424302491423	38.0	38.0	40.0	32.0	40.0
70-71	36.581854342111015	38.0	38.0	40.0	32.0	40.0
72-73	36.51516305239966	38.0	38.0	40.0	32.0	40.0
74-75	36.43687237013921	38.0	38.0	40.0	29.5	40.0
76-77	36.36352580552827	38.0	38.0	40.0	27.0	40.0
78-79	36.28519020786469	38.0	38.0	40.0	27.0	40.0
80-81	36.21729135784749	38.0	38.0	40.0	27.0	40.0
82-83	36.13311436558746	38.0	38.0	40.0	27.0	40.0
84-85	36.04344573320168	38.0	38.0	40.0	27.0	40.0
86-87	35.98606059331226	38.0	38.0	40.0	27.0	40.0
88-89	35.92254617130215	38.0	38.0	40.0	27.0	40.0
90-91	35.81538643332104	38.0	38.0	40.0	27.0	40.0
92-93	35.71959152967263	38.0	38.0	40.0	27.0	40.0
94-95	35.62730097609544	38.0	38.0	40.0	27.0	40.0
96-97	35.55647544830852	38.0	38.0	40.0	27.0	40.0
98-99	35.43353390597379	38.0	38.0	40.0	27.0	40.0
100-101	33.61578745486806	38.0	32.5	39.0	20.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.048021004097556386
2201	2	0.20213571204305936
2201	3	0.20811808084258843
2201	4	0.3166007024480564
2201	5	0.3211632825643207
2201	6	0.2925157173934494
2201	7	0.27461294905693734
2201	8	0.16497029037606126
2201	9	0.18665107678224757
2201	10-11	0.127000335219023
2201	12-13	0.16491500982749585
2201	14-15	0.17342516411648035
2201	16-17	0.13577880950711574
2201	18-19	0.17960996156923414
2201	20-21	0.21743399796971374
2201	22-23	0.16873057925786128
2201	24-25	0.15474163413458086
2201	26-27	0.19040543426283563
2201	28-29	0.21070118454482412
2201	30-31	0.17803960587322365
2201	32-33	0.18338069383744937
2201	34-35	0.17882358040664315
2201	36-37	0.10023235950436771
2201	38-39	0.13820802116523367
2201	40-41	0.06543109549826198
2201	42-43	0.11182764962582326
2201	44-45	0.13841132740250117
2201	46-47	0.13282554078870135
2201	48-49	0.058846850248201577
2201	50-51	0.11109913427564777
2201	52-53	0.1558512948419093
2201	54-55	0.20259136469486805
2201	56-57	0.03810792312992106
2201	58-59	0.08891564228865434
2201	60-61	0.036023083998891536
2201	62-63	0.11364168744547243
2201	64-65	0.08266354263287212
2201	66-67	0.05111900446788553
2201	68-69	-0.011226884346648092
2201	70-71	0.038005899589443004
2201	72-73	-0.06201701653660763
2201	74-75	0.01461378027915572
2201	76-77	-0.003143530286457974
2201	78-79	0.0205292933770167
2201	80-81	0.11571988684800516
2201	82-83	0.0439479371940763
2201	84-85	0.050269505553373506
2201	86-87	0.02131112823286685
2201	88-89	0.08708610221452773
2201	90-91	0.08567173039755716
2201	92-93	-0.10965500242274828
2201	94-95	0.006794445905335067
2201	96-97	-0.14663021935708542
2201	98-99	-0.060391566448622314
2201	100-101	0.05265961789243079
1101	1	0.03568825269765341
1101	2	0.13006219711126477
1101	3	0.17155865158709815
1101	4	0.1532734519015122
1101	5	0.18114067757988295
1101	6	0.23386870525432357
1101	7	0.20502140920066125
1101	8	0.20803895960259666
1101	9	0.2086654542274431
1101	10-11	0.16424038308314692
1101	12-13	0.1696828905220258
1101	14-15	0.1909632278967166
1101	16-17	0.18644387784778615
1101	18-19	0.20365850223316784
1101	20-21	0.22808799964298743
1101	22-23	0.17887580808163506
1101	24-25	0.17549245088086707
1101	26-27	0.18628688323448728
1101	28-29	0.17691230901344568
1101	30-31	0.17579701396674352
1101	32-33	0.12651099852042336
1101	34-35	0.1401881090385828
1101	36-37	0.16282325873092418
1101	38-39	0.13335136180720752
1101	40-41	0.14494368217101083
1101	42-43	0.12150597855675471
1101	44-45	0.06411211382246762
1101	46-47	0.05195316754983992
1101	48-49	0.08477362793233567
1101	50-51	0.0762318925658505
1101	52-53	0.13042487702217187
1101	54-55	0.06756553851281666
1101	56-57	0.1047708852596898
1101	58-59	0.037349612708922564
1101	60-61	0.04375648355843964
1101	62-63	0.048255894032855906
1101	64-65	0.05826124297119861
1101	66-67	0.050812529177505894
1101	68-69	-0.02916930575788257
1101	70-71	0.08812756548446288
1101	72-73	0.051070873402188965
1101	74-75	0.08760909001720307
1101	76-77	0.13277718735687927
1101	78-79	0.05246243776128523
1101	80-81	0.09000167242771795
1101	82-83	0.05418096504654102
1101	84-85	0.07915548296317354
1101	86-87	0.08849727077070924
1101	88-89	-0.019611218142642883
1101	90-91	0.005547596297766688
1101	92-93	0.06507275923747358
1101	94-95	-0.0013456782412717416
1101	96-97	-0.005606397899725835
1101	98-99	0.010350381337090653
1101	100-101	0.13477348728753213
2202	1	-0.018648546823961
2202	2	0.054736487191519245
2202	3	0.149026182994632
2202	4	0.0971821958248924
2202	5	0.08588581719521216
2202	6	0.06764106095194933
2202	7	0.13603428308616827
2202	8	0.07556875245457206
2202	9	0.1511538079855086
2202	10-11	0.12241176696051781
2202	12-13	0.09856574291486453
2202	14-15	0.05383222854833036
2202	16-17	0.04332522371711889
2202	18-19	0.09376024001795713
2202	20-21	0.09064966321817991
2202	22-23	0.06628634732902583
2202	24-25	0.03541374240434436
2202	26-27	0.029815769213890064
2202	28-29	0.03320307982642845
2202	30-31	0.04859172344369256
2202	32-33	0.07039337485749542
2202	34-35	0.036797678439050685
2202	36-37	0.06539453862487932
2202	38-39	0.01456031748632114
2202	40-41	0.03883996844886184
2202	42-43	-0.013040508617720548
2202	44-45	-0.0743528614299862
2202	46-47	-0.08969794876995252
2202	48-49	-0.02699875896617243
2202	50-51	-0.01536789585312448
2202	52-53	0.009574142088808912
2202	54-55	-0.052507862505684955
2202	56-57	-0.06254830861839622
2202	58-59	-0.04964733486200146
2202	60-61	-0.1284008314645675
2202	62-63	-0.014643752203561178
2202	64-65	-0.0928621535845906
2202	66-67	-0.08038238456897773
2202	68-69	-0.05863284574703442
2202	70-71	-0.04038872358454171
2202	72-73	-0.1387918101960821
2202	74-75	-0.06910628782772932
2202	76-77	-0.10534086519107433
2202	78-79	-0.07535845047279821
2202	80-81	-0.0832053395923964
2202	82-83	-0.10819141258406262
2202	84-85	-0.1325223685190764
2202	86-87	-0.1022429874393822
2202	88-89	-0.09608472233183107
2202	90-91	-0.018088551267958053
2202	92-93	-0.0770058856366731
2202	94-95	-0.0768707074108903
2202	96-97	-0.06603535088866863
2202	98-99	-0.09988987639968627
2202	100-101	-0.09698299062849003
1102	1	-0.19959863671444822
1102	2	0.0331442585340298
1102	3	-0.013889592875976575
1102	4	-0.07379105393025753
1102	5	-0.08170424735519077
1102	6	-0.15400828222801266
1102	7	0.0770493198114508
1102	8	0.07888430363454546
1102	9	0.11713625575603714
1102	10-11	0.044730235738917656
1102	12-13	0.13887445192102632
1102	14-15	0.13651510397062339
1102	16-17	0.12221554372470678
1102	18-19	0.09074934479967567
1102	20-21	0.1253312145490426
1102	22-23	0.11355188924633097
1102	24-25	0.11919071344971144
1102	26-27	0.1459998826727329
1102	28-29	0.013917873269107872
1102	30-31	0.08939378681067467
1102	32-33	0.03981865039744292
1102	34-35	0.11091281900258565
1102	36-37	0.09436888518237652
1102	38-39	0.10791975332845283
1102	40-41	0.07603053918918334
1102	42-43	-0.02637978493228843
1102	44-45	-0.05517102143744523
1102	46-47	-0.02490329999398
1102	48-49	-0.09441197564643744
1102	50-51	-0.05630026809734545
1102	52-53	-0.06148000804488163
1102	54-55	-0.09480043728765253
1102	56-57	-0.11816447207367986
1102	58-59	-0.07639697022275982
1102	60-61	-0.08987280980908707
1102	62-63	-0.12516773516968982
1102	64-65	-0.163456633872876
1102	66-67	-0.2139101999834594
1102	68-69	-0.26047725732617266
1102	70-71	-0.17838117961591848
1102	72-73	-0.16595100126256312
1102	74-75	-0.1329272617982653
1102	76-77	-0.1501769918785314
1102	78-79	-0.17261602288663624
1102	80-81	-0.0818527915938958
1102	82-83	-0.15517148410093284
1102	84-85	-0.1435437769100787
1102	86-87	-0.0885272001634334
1102	88-89	-0.256209017966583
1102	90-91	-0.3741710275881758
1102	92-93	-0.2319962908875155
1102	94-95	-0.17412310865726965
1102	96-97	-0.2319160531001998
1102	98-99	-0.3152677594239677
1102	100-101	-0.3303753131271492
2203	1	-0.21681325361404902
2203	2	-0.023967046590641417
2203	3	-0.026024437288100444
2203	4	-0.17704226643706278
2203	5	-0.08966870216483613
2203	6	-0.10510182210650498
2203	7	0.019858534154970187
2203	8	0.04863532491150124
2203	9	0.03548949529922396
2203	10-11	0.07289092417349963
2203	12-13	0.08091932031805271
2203	14-15	0.04638535562126833
2203	16-17	0.061890850052122914
2203	18-19	0.06673907005358615
2203	20-21	0.06992288392927293
2203	22-23	0.03414130096968648
2203	24-25	0.008180829224940567
2203	26-27	0.02312206186545751
2203	28-29	-0.019744692968203026
2203	30-31	-0.001804622830420044
2203	32-33	-0.028206958546135752
2203	34-35	-0.018215876168490297
2203	36-37	-0.04640757169087095
2203	38-39	-0.016265554518192005
2203	40-41	0.010990694701114023
2203	42-43	-0.0586471605145249
2203	44-45	-0.1078787677138493
2203	46-47	-0.2290325830849298
2203	48-49	-0.19367425200361765
2203	50-51	-0.1311825427094675
2203	52-53	-0.13187343645465432
2203	54-55	-0.12399206772264648
2203	56-57	-0.288868667358571
2203	58-59	-0.15821454478692942
2203	60-61	-0.229135027819666
2203	62-63	-0.21787892500861972
2203	64-65	-0.34343734186113295
2203	66-67	-0.32456231603403296
2203	68-69	-0.20890231462275466
2203	70-71	-0.17595973473066095
2203	72-73	-0.22262484055172393
2203	74-75	-0.2462898499355859
2203	76-77	-0.23703418110752494
2203	78-79	-0.30441029444477863
2203	80-81	-0.22350224824235454
2203	82-83	-0.32650462003571334
2203	84-85	-0.2922464069919215
2203	86-87	-0.34748915346309417
2203	88-89	-0.3641120073120021
2203	90-91	-0.29944849904634196
2203	92-93	-0.3609896197339566
2203	94-95	-0.28663787290651754
2203	96-97	-0.3798180543393741
2203	98-99	-0.4150769126149072
2203	100-101	-0.1770440833889495
1103	1	-0.22215318145342167
1103	2	-0.007539252486189696
1103	3	-0.05930902178059938
1103	4	-0.10700475549877098
1103	5	-0.10596511628958893
1103	6	-0.09039818187635973
1103	7	0.014144123508195605
1103	8	0.02695677191772461
1103	9	0.05337489849257793
1103	10-11	0.11937574306019627
1103	12-13	0.07660096600099564
1103	14-15	0.0805284119512919
1103	16-17	0.08431549832479135
1103	18-19	0.07083584113324548
1103	20-21	0.0968103900480699
1103	22-23	0.10825067150252465
1103	24-25	0.11634362196362247
1103	26-27	0.031518171506938586
1103	28-29	0.01124480862927868
1103	30-31	0.060141268423080874
1103	32-33	0.07979807848637677
1103	34-35	0.05049648578086163
1103	36-37	0.016683516638174467
1103	38-39	0.05296409165315197
1103	40-41	0.030343304724169684
1103	42-43	-0.05782980549020067
1103	44-45	-0.15419023346433391
1103	46-47	-0.12944401763490987
1103	48-49	-0.06165149502189138
1103	50-51	-0.12403833122517938
1103	52-53	-0.1281807360772973
1103	54-55	-0.0719825781418848
1103	56-57	-0.049296016291371814
1103	58-59	-0.14915877063241112
1103	60-61	-0.14426808404734714
1103	62-63	-0.2037552829330025
1103	64-65	-0.1257104086981755
1103	66-67	-0.23799926979801
1103	68-69	-0.18721381148350957
1103	70-71	-0.1331511360175952
1103	72-73	-0.15005912927663445
1103	74-75	-0.20872711697320057
1103	76-77	-0.3289961917728803
1103	78-79	-0.2805288828543766
1103	80-81	-0.23220971620728648
1103	82-83	-0.2825052988516603
1103	84-85	-0.21453474902020275
1103	86-87	-0.31411888001652954
1103	88-89	-0.2461631927773027
1103	90-91	-0.2557331384857733
1103	92-93	-0.20296318927200474
1103	94-95	-0.23716537439669594
1103	96-97	-0.2959240311026008
1103	98-99	-0.2606523044177038
1103	100-101	-0.19953593522945567
2204	1	-0.023468398231159426
2204	2	0.013276216688730358
2204	3	-0.0010464829012377663
2204	4	0.00272033016633344
2204	5	-0.05412590923909022
2204	6	-0.01225311141898544
2204	7	0.003899772974691018
2204	8	0.016883739663192898
2204	9	0.08006647327889027
2204	10-11	0.0996436982777098
2204	12-13	0.13300257946310268
2204	14-15	0.09616475442972217
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1213	8	-0.20487390784913373
1213	9	-0.22779291136213686
1213	10-11	-0.2804019441619019
1213	12-13	-0.19055038348999176
1213	14-15	-0.16703706893683545
1213	16-17	-0.14397266460647273
1213	18-19	-0.10957215995015446
1213	20-21	-0.2031596671274869
1213	22-23	-0.2019213429763269
1213	24-25	-0.2616355229793612
1213	26-27	-0.2123344245879082
1213	28-29	-0.18430994780589316
1213	30-31	-0.39424078318339184
1213	32-33	-0.26652278411994246
1213	34-35	-0.3350436712206033
1213	36-37	-0.34941215402263737
1213	38-39	-0.4878506080401763
1213	40-41	-0.3282249132435524
1213	42-43	-0.0867424045433225
1213	44-45	-0.13842764617606917
1213	46-47	-0.17348444392927576
1213	48-49	-0.08358568626597673
1213	50-51	-0.22445865099223283
1213	52-53	-0.21492712445415663
1213	54-55	-0.018522616297765637
1213	56-57	-0.10289395383656341
1213	58-59	-0.1385241002632398
1213	60-61	-0.0661419836483006
1213	62-63	-0.21598551072351313
1213	64-65	-0.2240464716695385
1213	66-67	-0.0674823336728565
1213	68-69	-0.09261495305955947
1213	70-71	-0.06770202417541071
1213	72-73	0.0347494604924492
1213	74-75	-0.07205146287056863
1213	76-77	-0.047393479276379935
1213	78-79	-0.037344446567324496
1213	80-81	0.04628188326283578
1213	82-83	0.08842650404892538
1213	84-85	0.10590897995662374
1213	86-87	0.060602747079961716
1213	88-89	0.035103205350594635
1213	90-91	0.02723237801811962
1213	92-93	-0.014409631686994828
1213	94-95	-0.15552967810896945
1213	96-97	-0.1139171957154943
1213	98-99	-0.019754765437163258
1213	100-101	-0.6952415758881969
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56.0
3	47.0
4	94.0
5	109.0
6	112.0
7	82.0
8	50.0
9	67.0
10	71.0
11	59.0
12	99.0
13	136.0
14	892.0
15	6346.0
16	26017.0
17	49068.0
18	55943.0
19	51132.0
20	48574.0
21	50265.0
22	55406.0
23	63007.0
24	71580.0
25	80974.0
26	91351.0
27	103431.0
28	117721.0
29	135163.0
30	155903.0
31	183312.0
32	218370.0
33	263738.0
34	332504.0
35	442517.0
36	643429.0
37	1115271.0
38	2790288.0
39	4969606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.199514696211642	34.77993710320361	34.301917161137816	15.718631039446937
2	16.189326835352404	36.657694547602276	32.56620203023409	14.586776586811231
3	16.301510966632097	34.43607195365167	33.433476561246536	15.828940518469704
4	16.560289403788534	33.57119253016974	33.342388063001124	16.526130003040606
5	16.652408561133036	33.827777484457705	33.84664438167395	15.673169572735313
6	17.161523589963842	34.03949472387831	32.526428458403586	16.27255322775425
7	17.126013533274698	33.891721097291025	33.29607319871559	15.686192170718682
8	16.32206988237167	34.396573735187154	33.411213237796325	15.870143144644851
9	16.564110644026638	33.7319673862114	33.5735839531185	16.13033801664346
10-11	16.317027998935327	34.24783479676584	33.30764040910857	16.12749679519027
12-13	16.278296300665634	34.1366562980422	33.58222844440521	16.002818956886955
14-15	16.36374918700039	34.12212793086256	33.42575797308986	16.08836490904719
16-17	16.481051375303007	33.86705825029176	33.63723057381719	16.014659800588035
18-19	16.31733937474343	33.76262883681472	33.56781654583943	16.35221524260242
20-21	16.580365042586763	33.761175539169955	33.28346671982105	16.374992698422233
22-23	16.499010881683592	33.714112976706694	33.26925830834425	16.517617833265458
24-25	16.760505210354626	33.504916740888326	33.294209588062444	16.4403684606946
26-27	16.817726847796973	33.566168360929275	33.18393360752917	16.432171183744586
28-29	16.696948657122068	33.5891834564657	33.092592918828935	16.621274967583304
30-31	16.678558529626088	33.449138376974005	33.18786120657012	16.684441886829788
32-33	16.777500690997794	33.53866664389128	33.06283023536505	16.621002429745875
34-35	16.888353710868977	33.60065145410769	32.90850621597857	16.60248861904476
36-37	16.81033732322226	33.59234812657573	32.980633918305266	16.61668063189675
38-39	16.804224878652775	33.489590286743	33.03408333480481	16.67210149979941
40-41	16.88582811009562	33.63983341749925	32.92433236625046	16.550006106154676
42-43	16.83087792832299	33.52302453831597	33.00245303938892	16.643644493972122
44-45	16.89989749549217	33.55967304502748	32.903295169663814	16.63713428981654
46-47	16.91073618317019	33.60868122506605	32.879647338846226	16.600935252917523
48-49	16.856234309153933	33.47363855353295	32.926127653479455	16.74399948383366
50-51	16.919601419043858	33.56511231250322	32.85818972678954	16.657096541663382
52-53	16.943071063392996	33.62523639940195	32.85019704075806	16.581495496446998
54-55	16.94942400183381	33.458664949700264	32.867970910918835	16.723940137547096
56-57	16.969689993918248	33.555526512313385	32.79479158957213	16.679991904196246
58-59	16.979158909831625	33.56253616275172	32.84547146945827	16.612833457958384
60-61	16.94832954209855	33.531914159744126	32.7496218048357	16.770134493321624
62-63	16.944980762282142	33.46187083412787	32.83526805197158	16.7578803516184
64-65	16.94780141755021	33.57733560036629	32.77491584027946	16.699947141804035
66-67	16.999865219719705	33.513754086262104	32.730114225771864	16.75626646824633
68-69	16.926776943720746	33.5003125135371	32.793487341654654	16.779423201087507
70-71	17.04305126681257	33.56344810329317	32.681831299731684	16.71166933016258
72-73	16.981773083935046	33.508510732581264	32.74197087426631	16.767745309217382
74-75	17.005721231184857	33.531873161703274	32.723990099757444	16.738415507354425
76-77	16.987984774509055	33.60327872207274	32.70297436408622	16.705762139331988
78-79	17.03035014600454	33.501433639376096	32.69308003600128	16.775136178618087
80-81	17.098295536107486	33.510282416397665	32.68373789182911	16.707684155665735
82-83	17.034982292453186	33.63581000767192	32.66944079192092	16.659766907953973
84-85	16.979380898776256	33.47051065782394	32.7411668971983	16.80894154620151
86-87	16.976746527602117	33.660329142145216	32.600236482145284	16.762687848107387
88-89	17.03677238350888	33.558225557516934	32.680742300325825	16.724259758648362
90-91	16.999446705454506	33.467168604298	32.68101826664307	16.85236642360442
92-93	17.011197307733827	33.56959411657705	32.656350637693244	16.76285793799587
94-95	17.017891953352194	33.5788070493234	32.632318833815596	16.770982163508812
96-97	17.025962923597493	33.51411217207133	32.671654612084666	16.788270292246512
98-99	17.051359564990047	33.538871900295945	32.60664404036716	16.803124494346857
100-101	17.09039312533727	33.610644819051814	32.485420956218874	16.813541099392044
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1484.0
1	1620.5
2	1971.0
3	2476.5
4	2994.0
5	3564.5
6	4292.5
7	5240.5
8	6751.5
9	8880.5
10	11835.5
11	16390.0
12	23532.0
13	34460.5
14	50014.0
15	71369.5
16	99655.5
17	134956.5
18	174816.5
19	218777.5
20	264267.5
21	307082.5
22	347356.5
23	382558.0
24	408528.5
25	424598.0
26	429897.5
27	427861.0
28	425675.0
29	421490.0
30	417135.0
31	415083.5
32	410462.0
33	402150.0
34	391449.0
35	377543.0
36	369307.5
37	366653.0
38	362080.0
39	354288.0
40	340121.5
41	318806.5
42	295343.0
43	271606.5
44	245521.0
45	222001.5
46	202484.0
47	191417.0
48	180704.5
49	163247.5
50	145375.5
51	128619.0
52	114743.0
53	102901.0
54	92187.0
55	82905.0
56	74882.0
57	67207.0
58	59816.0
59	52109.5
60	43655.5
61	35444.0
62	27919.0
63	21621.5
64	16713.5
65	12752.5
66	9384.0
67	6686.0
68	4793.0
69	3375.0
70	2257.0
71	1572.5
72	1159.0
73	857.0
74	638.0
75	511.5
76	376.5
77	276.0
78	230.5
79	183.0
80	127.0
81	86.5
82	61.5
83	48.5
84	34.0
85	21.5
86	20.0
87	16.5
88	15.5
89	13.0
90	8.0
91	9.0
92	8.5
93	6.5
94	6.0
95	6.0
96	8.0
97	8.0
98	8.0
99	11.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.104205385063999E-4
2	4.1244630980162155E-4
3	0.001262085707992962
4	0.0017487723535588755
5	0.008496393981913404
6	0.013000307684947114
7	0.014047921311843231
8	0.009362531232496809
9	0.0025571671207700536
10-11	0.0063970422650231505
12-13	0.010599870161901675
14-15	0.033721610289380574
16-17	0.01445211869544882
18-19	0.021954517070740316
20-21	0.017504221387980818
22-23	0.030219941119164814
24-25	0.025782018825699363
26-27	0.02161631109670299
28-29	0.030112705078616388
30-31	0.03183260619048915
32-33	0.03666235247826614
34-35	0.018250749208721755
36-37	0.028087593697490428
38-39	0.028343310409567432
40-41	0.03192334437864551
42-43	0.03928138654550644
44-45	0.027411181749415772
46-47	0.02800097997243209
48-49	0.021991637238622463
50-51	0.03587045556344703
52-53	0.028623773900232536
54-55	0.03085923289935733
56-57	0.024177602680571057
58-59	0.025592293523190618
60-61	0.03338340431534325
62-63	0.029935353165401697
64-65	0.029465164372227844
66-67	0.025278834327741386
68-69	0.02734519033984751
70-71	0.03045915997884975
72-73	0.03714491466073404
74-75	0.03212956753354632
76-77	0.03436502653267111
78-79	0.03572609935501646
80-81	0.04117451510749588
82-83	0.04303052350160318
84-85	0.04842119677071037
86-87	0.0496502867739192
88-89	0.05212908909582695
90-91	0.0517867586586916
92-93	0.05201772859218051
94-95	0.05297047956782226
96-97	0.053717007388563186
98-99	0.05488423044530178
100-101	0.05866636310618266
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.212279E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.77383709906753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.08557813396955	14.373266832214721
2	21.80624521962853	16.037986188978792
3	15.696638216216552	17.316768502984363
4	9.925436810563493	14.599855856350011
5	5.653170472033758	10.394438501591408
6	3.1152566667533197	6.8735964710982405
7	1.6026619055154019	4.125521948681318
8	0.9309444035051555	2.7387518274229747
9	0.5178348357014922	1.7138496503078169
>10	1.6100007574197002	10.364838866863538
>50	0.05116848632550574	1.2240470127742125
>100	0.005000586729089407	0.22168841057470598
>500	6.350563843579961E-5	0.015389930157861385
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.4746778588097294E-5	0.0	4.1244630980162156E-5
2	2.4746778588097294E-5	0.0	4.1244630980162156E-5
3	2.4746778588097294E-5	0.0	5.774248337222702E-5
4	2.4746778588097294E-5	0.0	5.774248337222702E-5
5	3.299570478412972E-5	0.0	6.599140956825945E-5
6	3.299570478412972E-5	0.0	9.898711435238918E-5
7	3.299570478412972E-5	1.649785239206486E-5	9.898711435238918E-5
8	4.1244630980162156E-5	1.649785239206486E-5	1.0723604054842161E-4
9	4.1244630980162156E-5	1.649785239206486E-5	1.7322745011668107E-4
10-11	6.599140956825945E-5	1.649785239206486E-5	1.7322745011668107E-4
12-13	9.073818815635674E-5	1.649785239206486E-5	1.7322745011668107E-4
14-15	1.1960942984247025E-4	2.8871241686113507E-5	1.7322745011668107E-4
16-17	1.608540608226324E-4	4.1244630980162156E-5	1.7322745011668107E-4
18-19	2.3096993348890806E-4	4.1244630980162156E-5	1.7322745011668107E-4
20-21	2.887124168611351E-4	4.1244630980162156E-5	1.7322745011668107E-4
22-23	3.547038264293946E-4	4.1244630980162156E-5	1.814763763127135E-4
24-25	4.536909407817837E-4	4.1244630980162156E-5	1.814763763127135E-4
26-27	5.196823503500432E-4	4.1244630980162156E-5	1.814763763127135E-4
28-29	6.021716123103674E-4	4.1244630980162156E-5	1.9384976560676213E-4
30-31	7.547767469369674E-4	4.1244630980162156E-5	2.062231549008108E-4
32-33	9.073818815635674E-4	4.1244630980162156E-5	2.1447208109684322E-4
34-35	0.0011301028888564431	4.1244630980162156E-5	2.2272100729287564E-4
36-37	0.0012662101710909782	4.1244630980162156E-5	2.2272100729287564E-4
38-39	0.0014105663795215457	4.1244630980162156E-5	2.2272100729287564E-4
40-41	0.0015920427558342592	4.1244630980162156E-5	2.2272100729287564E-4
42-43	0.0018312616155191997	4.1244630980162156E-5	2.3096993348890806E-4
44-45	0.0019962401394398484	4.1244630980162156E-5	2.4746778588097295E-4
46-47	0.0021570942002624807	4.1244630980162156E-5	2.804634906651027E-4
48-49	0.0024086864492414697	4.949355717619459E-5	2.804634906651027E-4
50-51	0.0026479053089264104	4.949355717619459E-5	3.0521026925319996E-4
52-53	0.002940742188885562	5.774248337222702E-5	3.217081216452648E-4
54-55	0.0032583258474328104	5.774248337222702E-5	3.217081216452648E-4
56-57	0.0035882828952741074	5.774248337222702E-5	3.5057936333137834E-4
58-59	0.003914115480017389	6.599140956825945E-5	3.959484574095567E-4
60-61	0.004219325749270589	6.599140956825945E-5	3.959484574095567E-4
62-63	0.004532784944719821	9.073818815635674E-5	4.0007292050757296E-4
64-65	0.004837995213973021	9.073818815635674E-5	4.454420145857513E-4
66-67	0.0051679522618143185	1.0723604054842161E-4	4.454420145857513E-4
68-69	0.005621643202596102	1.0723604054842161E-4	4.495664776837675E-4
70-71	0.005980471492123513	1.0723604054842161E-4	4.536909407817837E-4
72-73	0.006380544412631086	1.0723604054842161E-4	4.78437719369881E-4
74-75	0.006850733205804934	1.2785835603850267E-4	4.78437719369881E-4
76-77	0.007391037871645059	1.5260513462659998E-4	4.78437719369881E-4
78-79	0.00800145841015146	1.6497852392064862E-4	4.825621824678972E-4
80-81	0.008545887539089598	1.6497852392064862E-4	5.196823503500432E-4
82-83	0.009152183614497983	1.6497852392064862E-4	5.403046658401243E-4
84-85	0.009894586972140901	1.6497852392064862E-4	5.733003706242541E-4
86-87	0.010863835800174712	1.6497852392064862E-4	6.021716123103675E-4
88-89	0.011861955869894636	1.7322745011668104E-4	6.021716123103675E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCT	1435	0.0	26.150549	32-33
TCCGATC	1490	0.0	23.431856	32-33
GATCTGG	2610	0.0	19.830961	1
GACCGGA	460	1.891749E-10	15.48908	9
CGATCTA	1355	0.0	15.249185	34-35
GTGACTG	2410	0.0	14.580441	7
GGTGACT	2555	0.0	14.125206	6
TTCCGAT	2775	0.0	13.522003	30-31
CTGGTGA	5015	0.0	12.593374	4
CGGTGAC	460	7.093313E-7	12.387533	5
CAGACGT	1435	0.0	11.585949	18-19
TGACTGG	2975	0.0	11.494527	8
CTTCCGA	2985	0.0	11.377285	30-31
CGATCTG	820	0.0	10.137267	34-35
GCGACTG	440	9.100295E-4	9.7128315	7
TCTGGTG	5550	0.0	9.668227	3
TGGTGAC	5020	0.0	9.648456	5
TCTTCCG	3130	0.0	9.560731	28-29
AGACGCG	300	1.7022023E-5	9.50042	20-21
GACGTGT	1865	0.0	9.424008	20-21
>>END_MODULE