FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 4-59175_2015-03-25 191213422911.342000000044ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 4-59175_2015-03-25 191213422911.342000000044ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15188209
Sequences flagged as poor quality0
Sequence length101
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAC2550.035.382635
ATTGGCG16850.033.5367431
GCGAGAC6850.018.7175885
TGGCGTG19400.017.8688413
GGCGTGA18400.017.0334014
GCGTGCC2801.3432345E-515.2637475
TTGGCGT23300.015.0817282
GGCGTGC3657.1638533E-714.3111694
CCGATCT7150.013.28716232-33
TTGGCGA12350.013.073362
TTGGCGG14900.011.7920882
TCCGATC10200.011.642549532-33
ATAGGCG9450.011.0551521
GCGTGAC25900.011.0008985
TAGGCGT8157.585186E-1010.4879392
GGCGAGA19550.010.4447244
TGGCGAG20600.010.3733863
CGGGACT4155.314083E-410.2985346
CGAGACT6655.281836E-79.9974076
GCGTGGC4307.366987E-49.9391845
Produced by FastQC (version 0.11.2)