##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l02n02 4-59175_2015-03-25 191213422911.342000000044ae.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15188209
Sequences flagged as poor quality	0
Sequence length	101
%GC	30
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.92225870739598	38.0	38.0	38.0	32.0	38.0
2	36.01667629145741	38.0	38.0	38.0	32.0	38.0
3	35.84915772491674	38.0	32.0	38.0	32.0	38.0
4	35.990964372428635	38.0	38.0	38.0	32.0	38.0
5	35.77978575354079	38.0	32.0	38.0	32.0	38.0
6	38.17430712205764	40.0	38.0	40.0	38.0	40.0
7	38.25277963978505	40.0	38.0	40.0	38.0	40.0
8	38.35103118478288	40.0	38.0	40.0	38.0	40.0
9	38.348309007335885	40.0	38.0	40.0	38.0	40.0
10-11	38.35341276907633	40.0	38.0	40.0	38.0	40.0
12-13	38.31051380054093	40.0	38.0	40.0	38.0	40.0
14-15	38.29150830094582	40.0	38.0	40.0	38.0	40.0
16-17	38.27293287180865	40.0	38.0	40.0	38.0	40.0
18-19	38.249544004826376	40.0	38.0	40.0	38.0	40.0
20-21	38.245628269929654	40.0	38.0	40.0	38.0	40.0
22-23	38.22320818076707	40.0	38.0	40.0	38.0	40.0
24-25	38.17320850667778	40.0	38.0	40.0	38.0	40.0
26-27	38.1610207299623	40.0	38.0	40.0	38.0	40.0
28-29	38.09578571772353	40.0	38.0	40.0	38.0	40.0
30-31	38.063289983697224	40.0	38.0	40.0	38.0	40.0
32-33	38.068173640486506	40.0	38.0	40.0	38.0	40.0
34-35	37.99474645101341	40.0	38.0	40.0	38.0	40.0
36-37	38.01912727168819	40.0	38.0	40.0	38.0	40.0
38-39	37.96931214865426	40.0	38.0	40.0	38.0	40.0
40-41	37.94892956108255	40.0	38.0	40.0	38.0	40.0
42-43	37.842311624761024	40.0	38.0	40.0	38.0	40.0
44-45	37.78090731435155	40.0	38.0	40.0	35.0	40.0
46-47	37.716113203340825	40.0	38.0	40.0	32.0	40.0
48-49	37.64890824191318	40.0	38.0	40.0	32.0	40.0
50-51	37.598982078795466	40.0	38.0	40.0	32.0	40.0
52-53	37.53593991892	40.0	38.0	40.0	32.0	40.0
54-55	37.49268600399165	40.0	38.0	40.0	32.0	40.0
56-57	37.47571573448851	40.0	38.0	40.0	32.0	40.0
58-59	37.41463427320496	40.0	38.0	40.0	32.0	40.0
60-61	37.38773014645769	40.0	38.0	40.0	32.0	40.0
62-63	37.324607463592315	40.0	38.0	40.0	32.0	40.0
64-65	37.31816845554337	40.0	38.0	40.0	32.0	40.0
66-67	37.25600026309883	40.0	38.0	40.0	32.0	40.0
68-69	37.21195458266344	39.0	38.0	40.0	32.0	40.0
70-71	37.11490976322488	38.0	38.0	40.0	32.0	40.0
72-73	37.05587663430231	38.0	38.0	40.0	32.0	40.0
74-75	36.98244338749882	38.0	38.0	40.0	32.0	40.0
76-77	36.91345480563245	38.0	38.0	40.0	32.0	40.0
78-79	36.835282981686646	38.0	38.0	40.0	32.0	40.0
80-81	36.77329848437034	38.0	38.0	40.0	32.0	40.0
82-83	36.69646249929798	38.0	38.0	40.0	32.0	40.0
84-85	36.61204197940653	38.0	38.0	40.0	32.0	40.0
86-87	36.558361588255735	38.0	38.0	40.0	32.0	40.0
88-89	36.498659848570696	38.0	38.0	40.0	32.0	40.0
90-91	36.3965684828277	38.0	38.0	40.0	32.0	40.0
92-93	36.30658249435467	38.0	38.0	40.0	27.0	40.0
94-95	36.220178165839044	38.0	38.0	40.0	27.0	40.0
96-97	36.15701946819405	38.0	38.0	40.0	27.0	40.0
98-99	36.03321701722699	38.0	38.0	40.0	27.0	40.0
100-101	34.24959819159718	38.0	35.0	39.0	20.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.12066369521243558
2201	2	0.24340012862515437
2201	3	0.20184083199073655
2201	4	0.257201615370505
2201	5	0.23983605525457818
2201	6	0.2970575693646964
2201	7	0.300609338979406
2201	8	0.19394490467269065
2201	9	0.24428250356892534
2201	10-11	0.17504663440583812
2201	12-13	0.24028301405228802
2201	14-15	0.2339016231506008
2201	16-17	0.21331982517586567
2201	18-19	0.17985473680128905
2201	20-21	0.212789036274593
2201	22-23	0.19407666591938266
2201	24-25	0.25368863226839977
2201	26-27	0.2444919902327385
2201	28-29	0.20508365343940227
2201	30-31	0.18749261229370973
2201	32-33	0.19801003939595319
2201	34-35	0.23420898868605633
2201	36-37	0.12247807292650492
2201	38-39	0.16129772357750483
2201	40-41	0.1438413778513663
2201	42-43	0.2047576405746625
2201	44-45	0.14830489473700226
2201	46-47	0.18216301213792718
2201	48-49	0.11326419336867843
2201	50-51	0.21315877452929044
2201	52-53	0.16760077921134808
2201	54-55	0.23204154977602798
2201	56-57	0.07159787060760436
2201	58-59	0.15793061839769962
2201	60-61	0.09566783059865713
2201	62-63	0.17929197196084345
2201	64-65	0.12583185611030245
2201	66-67	0.15425345674680813
2201	68-69	0.11803163999063315
2201	70-71	0.16370085466781603
2201	72-73	0.06994186955500936
2201	74-75	0.08992550475752381
2201	76-77	0.07658960133607451
2201	78-79	0.1387847151119601
2201	80-81	0.20869201695917639
2201	82-83	0.15441925369556486
2201	84-85	0.10824551357008971
2201	86-87	0.134257920634397
2201	88-89	0.1281033370675857
2201	90-91	0.05568474060092399
2201	92-93	-0.01571194985471891
2201	94-95	0.0973724299081411
2201	96-97	-0.03785363574068157
2201	98-99	0.07593158472633377
2201	100-101	0.09717194117384054
1101	1	0.15015358981929694
1101	2	0.18702822191156088
1101	3	0.1502239640822367
1101	4	0.16669108885282924
1101	5	0.10638293451842173
1101	6	0.207322525214515
1101	7	0.20199573193890785
1101	8	0.20162546056458552
1101	9	0.18267890671488374
1101	10-11	0.22664869177751257
1101	12-13	0.18321545403843942
1101	14-15	0.19712261744751913
1101	16-17	0.1787139831899225
1101	18-19	0.20519464624761952
1101	20-21	0.24413195704291724
1101	22-23	0.24083243953359101
1101	24-25	0.19224190117406437
1101	26-27	0.21216183983120374
1101	28-29	0.25520229104083825
1101	30-31	0.20206312612383215
1101	32-33	0.14515278218441807
1101	34-35	0.21902605309639256
1101	36-37	0.19385541561944564
1101	38-39	0.19628504541383052
1101	40-41	0.21709084357699027
1101	42-43	0.16852414523039982
1101	44-45	0.12851651967010014
1101	46-47	0.15918017205530788
1101	48-49	0.13456336442914818
1101	50-51	0.15564236960121036
1101	52-53	0.20246262408345927
1101	54-55	0.10226594360543828
1101	56-57	0.16374634394357201
1101	58-59	0.08950476500573501
1101	60-61	0.04625974287384338
1101	62-63	0.10352645923170911
1101	64-65	0.1576983002192307
1101	66-67	0.1284960442510723
1101	68-69	0.08934123278824302
1101	70-71	0.14496350365626398
1101	72-73	0.10581651345291476
1101	74-75	0.17511846583303736
1101	76-77	0.1557995787524078
1101	78-79	0.1524783919740358
1101	80-81	0.2219747219565562
1101	82-83	0.1905770694258635
1101	84-85	0.12554758003380329
1101	86-87	0.18014180846610373
1101	88-89	0.13191415819721897
1101	90-91	0.09603431760854875
1101	92-93	0.14565097245168346
1101	94-95	0.06795772214322682
1101	96-97	0.09992729828099556
1101	98-99	0.10233920599158353
1101	100-101	0.21330964758196558
2202	1	0.06066627490659471
2202	2	0.03739003215658698
2202	3	0.06581604046208156
2202	4	0.08256864248422602
2202	5	0.027556299752568236
2202	6	0.16933564264147094
2202	7	0.22057928218278988
2202	8	0.15476596611201643
2202	9	0.21292397188877032
2202	10-11	0.16576675546701836
2202	12-13	0.15387456462445215
2202	14-15	0.16234083577959524
2202	16-17	0.14898458381018287
2202	18-19	0.1538677862574076
2202	20-21	0.17050442690560175
2202	22-23	0.17608675435769072
2202	24-25	0.16014443017462554
2202	26-27	0.12847672885376227
2202	28-29	0.1513217661564994
2202	30-31	0.13784490393801718
2202	32-33	0.16375512289194205
2202	34-35	0.14107733715889736
2202	36-37	0.18362489210157662
2202	38-39	0.15372232467966285
2202	40-41	0.12384186358899996
2202	42-43	0.11792608796623227
2202	44-45	0.04077590901972883
2202	46-47	0.024657015610834776
2202	48-49	0.05441519008351747
2202	50-51	0.15975489683599875
2202	52-53	0.11558350906057058
2202	54-55	0.13741894639736785
2202	56-57	0.10166436314568728
2202	58-59	0.1044731996388748
2202	60-61	0.03935764379898643
2202	62-63	0.048967062820921115
2202	64-65	0.08893895383743455
2202	66-67	0.08583516918108813
2202	68-69	0.07383433546912244
2202	70-71	0.03579453394956289
2202	72-73	-0.015658944289832277
2202	74-75	0.05074765771310297
2202	76-77	-0.02799448385326997
2202	78-79	-0.04740279465362818
2202	80-81	0.046725531524458574
2202	82-83	-0.0027158634137549598
2202	84-85	0.03167194167949816
2202	86-87	0.057146800213146776
2202	88-89	0.05828785838036765
2202	90-91	0.08116974978380398
2202	92-93	0.06022482551912134
2202	94-95	0.1168317016848377
2202	96-97	0.054605965617149366
2202	98-99	0.023896383260094467
2202	100-101	0.07713720680430924
1102	1	-0.07107461941608051
1102	2	-0.06476893805247386
1102	3	-0.14305313734549685
1102	4	-0.07811647417378964
1102	5	-0.12113270202522841
1102	6	-0.12289559273784079
1102	7	0.05688991415985356
1102	8	0.07793705422900388
1102	9	0.07609766112592808
1102	10-11	0.02274409330124172
1102	12-13	0.14113513050566695
1102	14-15	0.10150711547584734
1102	16-17	0.10086097136706229
1102	18-19	0.07389503584343515
1102	20-21	0.13231476983421686
1102	22-23	0.10477413729445573
1102	24-25	0.10316462702008522
1102	26-27	0.09550377911203611
1102	28-29	-0.022158911861062336
1102	30-31	0.04046569683634971
1102	32-33	0.029691499360204432
1102	34-35	0.05141440373525796
1102	36-37	0.0436233489571336
1102	38-39	0.1020627466477606
1102	40-41	0.08616345330292319
1102	42-43	0.00449311448873857
1102	44-45	0.014735860898021258
1102	46-47	0.02660187132031666
1102	48-49	-0.031542932177742955
1102	50-51	-0.00782597262779916
1102	52-53	-0.011407481348406634
1102	54-55	0.026917784528819766
1102	56-57	-0.06363848324523502
1102	58-59	-0.03134782569632932
1102	60-61	-0.01342331818636211
1102	62-63	-0.06606803947151008
1102	64-65	-0.13957536149199257
1102	66-67	-0.1510775871796568
1102	68-69	-0.2000420906071625
1102	70-71	-0.09448369535949297
1102	72-73	-0.08925115979324971
1102	74-75	-0.04462107229046808
1102	76-77	-0.10625918451482619
1102	78-79	-0.10975158529854667
1102	80-81	-0.044777513570487315
1102	82-83	-0.19240762618252205
1102	84-85	-0.05039226242627848
1102	86-87	-0.11290050847424737
1102	88-89	-0.21908239436805843
1102	90-91	-0.2934618276868264
1102	92-93	-0.18220494117669972
1102	94-95	-0.12377391056453746
1102	96-97	-0.13207217376989178
1102	98-99	-0.2327322639829248
1102	100-101	-0.13515428315474765
2203	1	-0.06568065486308683
2203	2	-0.061345286341818905
2203	3	-0.08082600954485031
2203	4	-0.12449070878823676
2203	5	-0.19606099217409678
2203	6	-0.07723997010894834
2203	7	0.0288533082627751
2203	8	0.02260557454126655
2203	9	0.06255692416487335
2203	10-11	0.04261521015875758
2203	12-13	0.08703915029326481
2203	14-15	0.0640812180576944
2203	16-17	0.05026369677383258
2203	18-19	0.06436187778483315
2203	20-21	0.12170989904168295
2203	22-23	0.04142339180027932
2203	24-25	0.02822328495761184
2203	26-27	0.017101207935503737
2203	28-29	-0.03575592844297404
2203	30-31	-0.011625362422655883
2203	32-33	-0.025764106836675182
2203	34-35	-0.019356264557309544
2203	36-37	-0.07349447717494684
2203	38-39	-0.0484524399852404
2203	40-41	-0.016063710532826292
2203	42-43	-0.032531921258083685
2203	44-45	-0.05449616623496922
2203	46-47	-0.130955503767602
2203	48-49	-0.13770509301537004
2203	50-51	-0.08983626281126078
2203	52-53	-0.08475481071499047
2203	54-55	-0.06374244338599055
2203	56-57	-0.1893439568329427
2203	58-59	-0.1102788444230498
2203	60-61	-0.1762549059794054
2203	62-63	-0.18603598264559906
2203	64-65	-0.22654647431357233
2203	66-67	-0.21720796505813666
2203	68-69	-0.15741160750617667
2203	70-71	-0.07821187970930055
2203	72-73	-0.12365317170501555
2203	74-75	-0.09485945169107168
2203	76-77	-0.15136089719111112
2203	78-79	-0.22807666659372927
2203	80-81	-0.143857277987415
2203	82-83	-0.2376840802709168
2203	84-85	-0.2459084147999846
2203	86-87	-0.3317359981480337
2203	88-89	-0.2907212347296735
2203	90-91	-0.22772080907541437
2203	92-93	-0.34824432321796195
2203	94-95	-0.23185948095694897
2203	96-97	-0.30259246297320175
2203	98-99	-0.3591608395297996
2203	100-101	-0.17583292500620473
1103	1	-0.175492869929343
1103	2	-0.10857286705299884
1103	3	-0.18027184906160443
1103	4	-0.13327863906887671
1103	5	-0.14388897615366858
1103	6	-0.10610768670150605
1103	7	0.01662925938264692
1103	8	0.017151457048754537
1103	9	0.053978503040390535
1103	10-11	0.061148675817030096
1103	12-13	0.03282898340294338
1103	14-15	0.028233349177021694
1103	16-17	0.015344114158104105
1103	18-19	0.00433483035554616
1103	20-21	0.023137206200438243
1103	22-23	-0.011720713657801696
1103	24-25	0.0031144470038313443
1103	26-27	-0.021250332664877192
1103	28-29	-0.028964771740561446
1103	30-31	0.01271467606875376
1103	32-33	-0.017479242492058233
1103	34-35	-0.005933241138293965
1103	36-37	-0.02372700130531058
1103	38-39	-0.038708308643464306
1103	40-41	-0.041338952482988134
1103	42-43	-0.11110515992255188
1103	44-45	-0.12204515673089844
1103	46-47	-0.12890670533966642
1103	48-49	-0.0590106953244387
1103	50-51	-0.09837748213459463
1103	52-53	-0.08053632030643598
1103	54-55	-0.07135129478510294
1103	56-57	-0.03356951776930828
1103	58-59	-0.09942409210493253
1103	60-61	-0.18570342998526712
1103	62-63	-0.17715512166838465
1103	64-65	-0.1514492760116397
1103	66-67	-0.251289689557467
1103	68-69	-0.20419756000719502
1103	70-71	-0.18047838379616366
1103	72-73	-0.16198802636326803
1103	74-75	-0.1893790111007263
1103	76-77	-0.2592476366959957
1103	78-79	-0.24593237770699972
1103	80-81	-0.24019054506673143
1103	82-83	-0.32897476341160115
1103	84-85	-0.23267785414569175
1103	86-87	-0.3290099646797984
1103	88-89	-0.28425075661483845
1103	90-91	-0.2924856696850924
1103	92-93	-0.26907737648790686
1103	94-95	-0.23059260921179003
1103	96-97	-0.3505154545239222
1103	98-99	-0.2667577034148678
1103	100-101	-0.17452654916148447
2204	1	-0.034076208534635555
2204	2	-0.005988771543826488
2204	3	0.018861932191995834
2204	4	-0.059411013443998684
2204	5	-0.005594790260929017
2204	6	0.041095576784165644
2204	7	0.08635504077026468
2204	8	0.06999410844547072
2204	9	0.0740034946078012
2204	10-11	0.08833371639262566
2204	12-13	0.15624832134638922
2204	14-15	0.11168674167771542
2204	16-17	0.09827521193198407
2204	18-19	0.13878829138977977
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1213	14-15	-0.22389714649614945
1213	16-17	-0.1942312809741864
1213	18-19	-0.15384424978285693
1213	20-21	-0.21858360187822967
1213	22-23	-0.23660562982659883
1213	24-25	-0.299303670603301
1213	26-27	-0.2094240759215964
1213	28-29	-0.23857434272765943
1213	30-31	-0.37809157968376184
1213	32-33	-0.29571852567172385
1213	34-35	-0.36829701557137895
1213	36-37	-0.40882891790494824
1213	38-39	-0.5230895186777218
1213	40-41	-0.3903808356429366
1213	42-43	-0.16408866228027108
1213	44-45	-0.2536708864173747
1213	46-47	-0.3378415035235989
1213	48-49	-0.21435487127081387
1213	50-51	-0.41845892086520564
1213	52-53	-0.3697157274468452
1213	54-55	-0.16887423542227964
1213	56-57	-0.25846390698785626
1213	58-59	-0.27091244750580756
1213	60-61	-0.1846521186366914
1213	62-63	-0.3430062490042829
1213	64-65	-0.36039845610068966
1213	66-67	-0.20899481119325003
1213	68-69	-0.18121757153496532
1213	70-71	-0.16309272847947653
1213	72-73	-0.13145826173096964
1213	74-75	-0.20107785528534095
1213	76-77	-0.1913026017740762
1213	78-79	-0.20705339779869547
1213	80-81	-0.12330681942155763
1213	82-83	-0.0438932827617009
1213	84-85	0.001590496776799455
1213	86-87	-0.015620291925593222
1213	88-89	-0.05612626485665828
1213	90-91	-0.07633745682984028
1213	92-93	-0.14166632581059702
1213	94-95	-0.19760782853260395
1213	96-97	-0.14791668252173196
1213	98-99	-0.17429651194277085
1213	100-101	-0.8438686424250221
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75.0
3	64.0
4	95.0
5	152.0
6	123.0
7	98.0
8	62.0
9	72.0
10	77.0
11	79.0
12	96.0
13	144.0
14	912.0
15	6255.0
16	25035.0
17	42919.0
18	46257.0
19	43121.0
20	42920.0
21	45245.0
22	51752.0
23	59342.0
24	68801.0
25	78872.0
26	91065.0
27	104570.0
28	120922.0
29	140402.0
30	166196.0
31	198255.0
32	239360.0
33	295781.0
34	377489.0
35	509153.0
36	747000.0
37	1288113.0
38	3175778.0
39	7221557.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.195935559091069	36.520829838276185	36.02132746418319	14.261907138449562
2	14.21291405174222	38.04459769854606	34.64084932596741	13.101638923744307
3	14.253717182440695	35.92058510472293	35.31248588522162	14.513211827614755
4	15.000563275751036	35.06655904621185	35.37608746622239	14.556790211814727
5	15.27110952962221	35.27306649044362	35.350265039131976	14.105558940802199
6	14.93859865732921	35.55873608518288	34.37546053476358	15.127204722724324
7	15.158504602468748	35.29227307965863	35.19008745438013	14.359134863492503
8	14.65512212036581	35.84921264856219	35.069163634744996	14.426501596327007
9	14.702158589992212	35.23925832464162	35.32767121256485	14.730911872801325
10-11	14.582147103759485	35.669617520243314	35.12553465311363	14.62270072288357
12-13	14.510481866655148	35.52726849866672	35.345842205184994	14.616407429493142
14-15	14.589873833577402	35.4700716500509	35.26160112894968	14.678453387422033
16-17	14.684459989799128	35.31296361701502	35.3811055127011	14.621470880484758
18-19	14.669330915910422	35.165920079569226	35.23413976988976	14.930609234630591
20-21	14.824734130184106	35.09201697291687	35.08885606002811	14.994392836870915
22-23	14.801402493913798	35.20941185985546	34.9915408307057	14.997644815525039
24-25	15.048655040808697	34.98813663545406	34.893992356893946	15.0692159668433
26-27	15.036647596890065	35.013751615846964	34.88838579678666	15.061214990476307
28-29	15.021186428119766	35.049396580152155	34.80515184172124	15.12426515000684
30-31	14.965103613953945	34.97569429293798	34.83420203136205	15.22500006174603
32-33	15.073043897736568	35.01560413512545	34.72867207631094	15.182679890827039
34-35	15.097379297628386	35.11495546279429	34.662446587044904	15.125218652532418
36-37	15.083209530942748	34.957990609404135	34.7017999273239	15.256999932329226
38-39	15.167673733073931	35.01975149350901	34.63824262304497	15.174332150372088
40-41	15.150057901081276	35.087969632270095	34.63815405458229	15.123818412066345
42-43	15.098244856563744	34.91823673186025	34.71848824527976	15.265030166296247
44-45	15.199245333792502	35.04517588214884	34.58212387220003	15.17345491185863
46-47	15.229084705378645	35.04419047831052	34.59407138647554	15.132653429835297
48-49	15.167947570671652	34.90746509490899	34.59583404662964	15.328753287789715
50-51	15.257908285517896	34.93674767913236	34.55317254841107	15.252171486938673
52-53	15.237136570449575	35.05885872953238	34.539725522081255	15.164279177936784
54-55	15.217453586132319	34.92818198660686	34.57076644058493	15.283597986675884
56-57	15.277073158427775	34.9689619726829	34.51193398065273	15.242030888236593
58-59	15.292017575979495	34.98245233911735	34.55929324647203	15.166236838431121
60-61	15.281112397373144	34.94304308550438	34.4752433454052	15.300601171717275
62-63	15.253393775422033	34.91785258909566	34.51653683428523	15.312216801197076
64-65	15.207431543973152	35.06953604187722	34.54727933520535	15.175753078944282
66-67	15.311648132656567	34.9426988655681	34.48497140746905	15.260681594306288
68-69	15.318011971374732	34.89943040887242	34.518345213878625	15.264212405874222
70-71	15.30829257494829	35.02400323030927	34.470115967562684	15.197588227179759
72-73	15.306589652263119	34.93029477893943	34.450953753716135	15.312161815081321
74-75	15.333284595960595	35.00485397887959	34.447097557730736	15.214763867429076
76-77	15.312159952331129	35.01655972356322	34.49921058473437	15.17206973937128
78-79	15.356981514631652	34.88869003909866	34.4834241846059	15.270904261663784
80-81	15.371801355528245	34.958261038186194	34.48795341423891	15.181984192046649
82-83	15.329353879736715	35.09128816490476	34.42562632426833	15.153731631090192
84-85	15.250762039780605	34.9102619688365	34.53196588412094	15.307010107261949
86-87	15.372805422008708	35.07875032495829	34.376998516118505	15.171445736914494
88-89	15.35303755049508	35.07207532008791	34.463446676344475	15.111440453072536
90-91	15.306067733228215	34.94821249582359	34.44426651144995	15.30145325949824
92-93	15.37390906238858	35.026324132087545	34.43581182062141	15.16395498490247
94-95	15.325259360799237	35.109596970442155	34.42802945738691	15.1371142113717
96-97	15.288957636331693	35.00050493402558	34.50569923283624	15.204838196806486
98-99	15.384192511307452	35.02028618778365	34.42030396113105	15.175217339777852
100-101	15.361181168222457	35.14202610557061	34.40115094847943	15.095641777727506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1536.0
1	1741.0
2	2187.5
3	2655.5
4	3175.0
5	3789.5
6	4581.5
7	5729.5
8	7178.5
9	9168.5
10	12475.0
11	17718.0
12	25582.0
13	38094.0
14	57489.5
15	85715.5
16	124692.5
17	175218.5
18	236293.5
19	306829.0
20	383873.5
21	461276.0
22	538170.0
23	608247.5
24	664356.0
25	705117.0
26	727797.5
27	736245.0
28	742070.5
29	746616.0
30	741022.5
31	729084.5
32	707889.5
33	666760.0
34	607652.0
35	533679.5
36	457844.0
37	392072.0
38	336171.5
39	291183.0
40	256678.0
41	231184.0
42	217153.0
43	209482.5
44	195378.0
45	175746.0
46	157436.0
47	148363.0
48	135318.5
49	109823.5
50	84330.0
51	65157.5
52	52643.0
53	42663.5
54	35004.5
55	29194.0
56	24879.5
57	21504.5
58	18694.5
59	16135.0
60	13373.0
61	10682.5
62	8515.0
63	6836.0
64	5455.0
65	4338.5
66	3407.5
67	2622.0
68	2015.5
69	1507.0
70	1083.5
71	820.0
72	607.5
73	457.5
74	364.0
75	277.5
76	216.5
77	146.5
78	100.5
79	73.5
80	58.5
81	42.0
82	24.0
83	21.5
84	21.5
85	11.0
86	7.5
87	8.5
88	5.5
89	6.0
90	6.5
91	6.5
92	5.5
93	5.0
94	6.0
95	5.5
96	7.5
97	9.0
98	8.5
99	11.0
100	30.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.123170941353256E-4
2	4.67467889071055E-4
3	0.0014945804340722466
4	0.0017513585703225441
5	0.009184756412029885
6	0.013556568783060595
7	0.013958196124375166
8	0.009685144574979182
9	0.0024887727051951945
10-11	0.00598161376367681
12-13	0.01054107169581351
14-15	0.03454653540782853
16-17	0.014817415272597317
18-19	0.02267219261994617
20-21	0.017925089126703483
22-23	0.03084629662391398
24-25	0.02678393482733876
26-27	0.02270182086643659
28-29	0.03073436769272796
30-31	0.033226432425310974
32-33	0.03749619194731914
34-35	0.018906113288275135
36-37	0.02897971709501759
38-39	0.02910481413575491
40-41	0.03283138913877206
42-43	0.04027137103525504
44-45	0.028163294302837157
46-47	0.02856492164415172
48-49	0.022553679633984497
50-51	0.03637690263545886
52-53	0.02921674306694094
54-55	0.03135326884163893
56-57	0.02429845414953139
58-59	0.025342026831471704
60-61	0.03364122787617684
62-63	0.029819184078912795
64-65	0.02892046060203675
66-67	0.024601320669211228
68-69	0.02680697901905353
70-71	0.029782971777646727
72-73	0.036834494442366444
74-75	0.03153103832058145
76-77	0.03381241330034371
78-79	0.035132516282861265
80-81	0.04102524530706682
82-83	0.04264163075448857
84-85	0.047688308740023264
86-87	0.04881747413404701
88-89	0.05115810560679011
90-91	0.050782814484578144
92-93	0.051003380319562364
94-95	0.052066705165829626
96-97	0.052725110643394496
98-99	0.05383123184570347
100-101	0.05746892210924936
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.5188209E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.060924127987434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.62092550217994	41.11876198804515
2	18.529632381787497	21.88754424440501
3	6.164084998576056	10.92169669258098
4	2.5103255085954634	5.930485775988325
5	1.1164645394667927	3.2969713728518335
6	0.6627232247502653	2.3484627656898356
7	0.3203671568640347	1.3244826241252066
8	0.21426426914183438	1.0123716594499648
9	0.15052434170068818	0.8001096052139641
>10	0.6284954789396571	6.9405685451917405
>50	0.058862660124604956	2.415238113502304
>100	0.023329937873067128	2.0033066129557313
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.6336219102594652E-5	0.0	0.0
2	2.6336219102594652E-5	0.0	1.3168109551297326E-5
3	2.6336219102594652E-5	6.584054775648663E-6	1.975216432694599E-5
4	3.2920273878243316E-5	6.584054775648663E-6	1.975216432694599E-5
5	3.2920273878243316E-5	6.584054775648663E-6	3.2920273878243316E-5
6	3.2920273878243316E-5	6.584054775648663E-6	3.950432865389198E-5
7	3.2920273878243316E-5	6.584054775648663E-6	4.608838342954064E-5
8	3.2920273878243316E-5	6.584054775648663E-6	5.9256492980837965E-5
9	3.2920273878243316E-5	6.584054775648663E-6	7.242460253213529E-5
10-11	3.2920273878243316E-5	1.3168109551297326E-5	7.571662991995962E-5
12-13	5.2672438205189305E-5	1.3168109551297326E-5	8.888473947125694E-5
14-15	7.900865730778395E-5	1.3168109551297326E-5	1.053448764103786E-4
16-17	1.0205284902255427E-4	1.3168109551297326E-5	1.152209585738516E-4
18-19	1.152209585738516E-4	1.3168109551297326E-5	1.2838906812514893E-4
20-21	1.678933967790409E-4	1.3168109551297326E-5	1.7118542416686523E-4
22-23	2.1727380759640585E-4	1.3168109551297326E-5	2.1398178020858155E-4
24-25	2.6665421841377086E-4	1.3168109551297326E-5	2.2385786237205454E-4
26-27	3.3249476617025746E-4	1.3168109551297326E-5	2.3702597192335186E-4
28-29	4.3783964258063603E-4	1.975216432694599E-5	2.6665421841377086E-4
30-31	5.398924916031904E-4	1.975216432694599E-5	2.7653030057724385E-4
32-33	5.826888476449067E-4	1.975216432694599E-5	2.9957449229201416E-4
34-35	6.880337240552852E-4	1.975216432694599E-5	3.2920273878243313E-4
36-37	7.900865730778395E-4	2.6336219102594652E-5	3.3249476617025746E-4
38-39	9.151836138151641E-4	2.6336219102594652E-5	3.5224693049720345E-4
40-41	0.001004068353286421	3.2920273878243316E-5	3.687070674363251E-4
42-43	0.0011258733666359214	3.2920273878243316E-5	4.0491936870239273E-4
44-45	0.0012674305443123677	3.6212301266067646E-5	4.2137950564151444E-4
46-47	0.0014155717767644624	4.608838342954064E-5	4.2137950564151444E-4
48-49	0.001580173146155679	5.9256492980837965E-5	4.279635604171631E-4
50-51	0.0018040310085277336	6.913257514431096E-5	4.510077521319334E-4
52-53	0.001975216432694599	7.242460253213529E-5	5.003881629492984E-4
54-55	0.002133233747310167	7.571662991995962E-5	5.069722177249471E-4
56-57	0.002274790924986613	7.900865730778395E-5	5.069722177249471E-4
58-59	0.002534861088624735	9.217676685908127E-5	5.333084368275417E-4
60-61	0.0027587189509967894	9.217676685908127E-5	5.497685737666634E-4
62-63	0.0030550014159009794	9.217676685908127E-5	5.793968202570823E-4
64-65	0.003318363606926926	9.876082163472993E-5	6.05733039359677E-4
66-67	0.0036475663457093592	1.1192893118602727E-4	6.090250667475013E-4
68-69	0.003960308947552671	1.1192893118602727E-4	6.1560912152315E-4
70-71	0.004342184124540293	1.1851298596167593E-4	6.25485203686623E-4
72-73	0.004714183219364443	1.1851298596167593E-4	6.287772310744473E-4
74-75	0.005207987327538092	1.1851298596167593E-4	6.35361285850096E-4
76-77	0.005698499408323917	1.1851298596167593E-4	6.551134501770419E-4
78-79	0.006340444748949662	1.1851298596167593E-4	6.781576418918123E-4
80-81	0.007094319020761434	1.1851298596167593E-4	6.913257514431096E-4
82-83	0.008091803319272207	1.250970407373246E-4	6.979098062187582E-4
84-85	0.009267057096725493	1.349731229007976E-4	7.242460253213529E-4
86-87	0.010738593339082968	1.4484920506427059E-4	7.407061622604745E-4
88-89	0.012901455332883553	1.4814123245209492E-4	7.637503539752448E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGAC	255	0.0	35.38263	5
ATTGGCG	1685	0.0	33.536743	1
GCGAGAC	685	0.0	18.717588	5
TGGCGTG	1940	0.0	17.868841	3
GGCGTGA	1840	0.0	17.033401	4
GCGTGCC	280	1.3432345E-5	15.263747	5
TTGGCGT	2330	0.0	15.081728	2
GGCGTGC	365	7.1638533E-7	14.311169	4
CCGATCT	715	0.0	13.287162	32-33
TTGGCGA	1235	0.0	13.07336	2
TTGGCGG	1490	0.0	11.792088	2
TCCGATC	1020	0.0	11.6425495	32-33
ATAGGCG	945	0.0	11.055152	1
GCGTGAC	2590	0.0	11.000898	5
TAGGCGT	815	7.585186E-10	10.487939	2
GGCGAGA	1955	0.0	10.444724	4
TGGCGAG	2060	0.0	10.373386	3
CGGGACT	415	5.314083E-4	10.298534	6
CGAGACT	665	5.281836E-7	9.997407	6
GCGTGGC	430	7.366987E-4	9.939184	5
>>END_MODULE