##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l02n02 3-58925.34200000001ae4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13790623
Sequences flagged as poor quality	0
Sequence length	101
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.63745793065331	38.0	32.0	38.0	32.0	38.0
2	36.071513447942124	38.0	38.0	38.0	32.0	38.0
3	35.59242871043607	38.0	32.0	38.0	32.0	38.0
4	35.98708259953158	38.0	38.0	38.0	32.0	38.0
5	35.80614385586496	38.0	38.0	38.0	32.0	38.0
6	38.01836189706585	40.0	38.0	40.0	38.0	40.0
7	38.12144810281595	40.0	38.0	40.0	38.0	40.0
8	38.204222608362215	40.0	38.0	40.0	38.0	40.0
9	38.211323665363054	40.0	38.0	40.0	38.0	40.0
10-11	38.23490363705831	40.0	38.0	40.0	38.0	40.0
12-13	38.19651885922775	40.0	38.0	40.0	38.0	40.0
14-15	38.159498051683386	40.0	38.0	40.0	38.0	40.0
16-17	38.148517329492655	40.0	38.0	40.0	38.0	40.0
18-19	38.128319547274984	40.0	38.0	40.0	38.0	40.0
20-21	38.11102025630024	40.0	38.0	40.0	38.0	40.0
22-23	38.09382244732526	40.0	38.0	40.0	38.0	40.0
24-25	38.04008444723635	40.0	38.0	40.0	38.0	40.0
26-27	38.02531430233428	40.0	38.0	40.0	38.0	40.0
28-29	37.9596838736002	40.0	38.0	40.0	38.0	40.0
30-31	37.92921044973821	40.0	38.0	40.0	38.0	40.0
32-33	37.93661221831675	40.0	38.0	40.0	38.0	40.0
34-35	37.86063936342832	40.0	38.0	40.0	38.0	40.0
36-37	37.87166631268218	40.0	38.0	40.0	38.0	40.0
38-39	37.82745087005859	40.0	38.0	40.0	32.0	40.0
40-41	37.7550440977177	40.0	38.0	40.0	32.0	40.0
42-43	37.572406554801766	40.0	38.0	40.0	32.0	40.0
44-45	37.4943138536961	40.0	38.0	40.0	32.0	40.0
46-47	37.42604191993357	40.0	38.0	40.0	32.0	40.0
48-49	37.35446701718987	40.0	38.0	40.0	32.0	40.0
50-51	37.29995860230535	40.0	38.0	40.0	32.0	40.0
52-53	37.2316381210624	40.0	38.0	40.0	32.0	40.0
54-55	37.18534895051515	40.0	38.0	40.0	32.0	40.0
56-57	37.17005874208874	39.0	38.0	40.0	32.0	40.0
58-59	37.10540731191042	38.0	38.0	40.0	32.0	40.0
60-61	37.07190378563753	38.0	38.0	40.0	32.0	40.0
62-63	37.00242708396858	38.0	38.0	40.0	32.0	40.0
64-65	36.993023447889186	38.0	38.0	40.0	32.0	40.0
66-67	36.93122518830367	38.0	38.0	40.0	32.0	40.0
68-69	36.88405915381777	38.0	38.0	40.0	32.0	40.0
70-71	36.781558237071664	38.0	38.0	40.0	32.0	40.0
72-73	36.71607323323971	38.0	38.0	40.0	32.0	40.0
74-75	36.63723723721546	38.0	38.0	40.0	32.0	40.0
76-77	36.56569637934414	38.0	38.0	40.0	32.0	40.0
78-79	36.487139667294215	38.0	38.0	40.0	32.0	40.0
80-81	36.42215917293947	38.0	38.0	40.0	32.0	40.0
82-83	36.339981812279255	38.0	38.0	40.0	27.0	40.0
84-85	36.24814136388182	38.0	38.0	40.0	27.0	40.0
86-87	36.190320335781784	38.0	38.0	40.0	27.0	40.0
88-89	36.130824256453096	38.0	38.0	40.0	27.0	40.0
90-91	36.02323564352386	38.0	38.0	40.0	27.0	40.0
92-93	35.92895991718431	38.0	38.0	40.0	27.0	40.0
94-95	35.83636072859072	38.0	38.0	40.0	27.0	40.0
96-97	35.768417170130746	38.0	38.0	40.0	27.0	40.0
98-99	35.64361577428373	38.0	38.0	40.0	27.0	40.0
100-101	33.79161554195194	38.0	32.5	39.0	20.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.14342571101708756
2201	2	0.2407833840597604
2201	3	0.32791999132961536
2201	4	0.2217818516556349
2201	5	0.26424029783710523
2201	6	0.31689463084114067
2201	7	0.2950953938340035
2201	8	0.1455364272742372
2201	9	0.22705440331651516
2201	10-11	0.14610350593911647
2201	12-13	0.20915558663336498
2201	14-15	0.19790650756825556
2201	16-17	0.16904763987112403
2201	18-19	0.15910714837184514
2201	20-21	0.20145499220411978
2201	22-23	0.16150827124837264
2201	24-25	0.19050480969106331
2201	26-27	0.20482012236747948
2201	28-29	0.2292428293062656
2201	30-31	0.20458447413690095
2201	32-33	0.19632383896693284
2201	34-35	0.2098469198490207
2201	36-37	0.12799436689099508
2201	38-39	0.1878247526063035
2201	40-41	0.1800790684013336
2201	42-43	0.1864420607387558
2201	44-45	0.15481971069406342
2201	46-47	0.18128350917088198
2201	48-49	0.060418634936212356
2201	50-51	0.1667467363618158
2201	52-53	0.16405943163800885
2201	54-55	0.18617263557509034
2201	56-57	0.1029611437750475
2201	58-59	0.17277910574751587
2201	60-61	0.05882002017365551
2201	62-63	0.1939085103612257
2201	64-65	0.10747017948826709
2201	66-67	0.14308790575381636
2201	68-69	0.04750038837861581
2201	70-71	0.12046336843371819
2201	72-73	0.06294967591967549
2201	74-75	0.07606415242791087
2201	76-77	0.06168525287401394
2201	78-79	0.05797940489321718
2201	80-81	0.2102800253100341
2201	82-83	0.16146634173507834
2201	84-85	0.11530809499183903
2201	86-87	0.12593350446102392
2201	88-89	0.11400890013907627
2201	90-91	0.0702407499841442
2201	92-93	-0.01263902345326784
2201	94-95	0.14337364309344025
2201	96-97	-0.0436040842632579
2201	98-99	0.0185934505583063
2201	100-101	0.16128330119236978
1101	1	0.11716718299779671
1101	2	0.23576784454602517
1101	3	0.14343418822524256
1101	4	0.10871302351779377
1101	5	0.18197874680480908
1101	6	0.19521662224767056
1101	7	0.23646448441718348
1101	8	0.2129907019513766
1101	9	0.28115794085518075
1101	10-11	0.25170644967165146
1101	12-13	0.20503433011878514
1101	14-15	0.20157343509501402
1101	16-17	0.21866632544578835
1101	18-19	0.24442117993413603
1101	20-21	0.25520878867527585
1101	22-23	0.27476021266750195
1101	24-25	0.21506505031644707
1101	26-27	0.21604065214140888
1101	28-29	0.23867462893043978
1101	30-31	0.2573996807890282
1101	32-33	0.21045604016182295
1101	34-35	0.19794225844514557
1101	36-37	0.1983541784151157
1101	38-39	0.1872571638118643
1101	40-41	0.18071249599511674
1101	42-43	0.18496071589380847
1101	44-45	0.17763417256764313
1101	46-47	0.14351593410888341
1101	48-49	0.15124076821648913
1101	50-51	0.16239014764645532
1101	52-53	0.193653446657585
1101	54-55	0.1485974225557527
1101	56-57	0.16586971789275395
1101	58-59	0.16126411642252236
1101	60-61	0.11342211847935602
1101	62-63	0.17377073477088345
1101	64-65	0.1538932632335488
1101	66-67	0.12536152895233243
1101	68-69	0.07230768161791445
1101	70-71	0.16218700737670844
1101	72-73	0.14089025910004693
1101	74-75	0.139185935821736
1101	76-77	0.1875432560589303
1101	78-79	0.1509452106465261
1101	80-81	0.22734943989063794
1101	82-83	0.23014021474395463
1101	84-85	0.1758528976897722
1101	86-87	0.2358610133071508
1101	88-89	0.1974345522447578
1101	90-91	0.13615279839061856
1101	92-93	0.17490862150347652
1101	94-95	0.10059024711523534
1101	96-97	0.06939773256492288
1101	98-99	0.07079028464913506
1101	100-101	0.18933666579514608
2202	1	0.00636045412135644
2202	2	0.11361295970294805
2202	3	0.03964909647560688
2202	4	0.09027811747960612
2202	5	0.129628282056089
2202	6	0.07977445665920868
2202	7	0.17544448986468097
2202	8	0.16848962362555397
2202	9	0.18717049966230093
2202	10-11	0.17924965701929807
2202	12-13	0.13367338996479816
2202	14-15	0.1452841681165964
2202	16-17	0.1439251202759948
2202	18-19	0.14324676928379887
2202	20-21	0.1671385477721259
2202	22-23	0.18213326097736626
2202	24-25	0.1627762505177941
2202	26-27	0.1432230093257587
2202	28-29	0.12711401187039684
2202	30-31	0.12923816926728904
2202	32-33	0.20158142976322324
2202	34-35	0.15941985574216488
2202	36-37	0.15376257543896799
2202	38-39	0.15509313482016296
2202	40-41	0.11461813940763221
2202	42-43	0.09103858333037351
2202	44-45	0.05736634350596859
2202	46-47	0.011594145477225481
2202	48-49	0.05256395414852477
2202	50-51	0.11390930072745675
2202	52-53	0.11072253207446181
2202	54-55	0.08912721741951657
2202	56-57	0.061438177958990536
2202	58-59	0.06916357628360714
2202	60-61	0.008275971409986482
2202	62-63	0.042502320651408354
2202	64-65	0.035467298924096724
2202	66-67	0.024705083555659257
2202	68-69	0.02741886999565679
2202	70-71	0.033445302868116755
2202	72-73	-0.011310119127834639
2202	74-75	0.029810760331237418
2202	76-77	-0.03155351599368572
2202	78-79	-4.928012445333252E-4
2202	80-81	0.04909423150279224
2202	82-83	-0.030013735169220013
2202	84-85	0.002412797937061839
2202	86-87	0.02057885803239401
2202	88-89	-0.027483624095964387
2202	90-91	0.04461806613803532
2202	92-93	-0.009273204310460414
2202	94-95	0.08494904956106097
2202	96-97	0.001316345036769917
2202	98-99	0.005839383516864416
2202	100-101	-0.020402910094773574
1102	1	-0.149695079810364
1102	2	0.047368499724377955
1102	3	-0.1538912456543855
1102	4	-0.08500889693958413
1102	5	-0.16654505838513955
1102	6	-0.17131874397538382
1102	7	0.02221923579313767
1102	8	0.03816900762194564
1102	9	0.0840207082460509
1102	10-11	0.003255224238721155
1102	12-13	0.1155477065927144
1102	14-15	0.03572059201781741
1102	16-17	0.035766528148222676
1102	18-19	0.018977391180278858
1102	20-21	0.06385779011338144
1102	22-23	0.0878765909468271
1102	24-25	0.05205063829004075
1102	26-27	0.06484591333600775
1102	28-29	-0.08674114228047358
1102	30-31	-0.001944775801035803
1102	32-33	6.971966446158717E-4
1102	34-35	0.04937718085689369
1102	36-37	0.04940155547782865
1102	38-39	0.06082015550540376
1102	40-41	0.06357661213831278
1102	42-43	-0.02309970611813128
1102	44-45	-0.042058462685545805
1102	46-47	-0.04428815065192282
1102	48-49	-0.11503104119697127
1102	50-51	-0.10443195740494815
1102	52-53	-0.08238157646149347
1102	54-55	-0.07017744607976795
1102	56-57	-0.14689329377054605
1102	58-59	-0.11350154101740628
1102	60-61	-0.13882545336063856
1102	62-63	-0.17785694126609286
1102	64-65	-0.16470966743679583
1102	66-67	-0.1885107133604862
1102	68-69	-0.2751670348672377
1102	70-71	-0.1574098931550978
1102	72-73	-0.17645640022369946
1102	74-75	-0.13468611458517188
1102	76-77	-0.16107449104708849
1102	78-79	-0.1850897846966859
1102	80-81	-0.15894618497542012
1102	82-83	-0.16361363351985858
1102	84-85	-0.1785242635289066
1102	86-87	-0.1390171832475815
1102	88-89	-0.30546713200745756
1102	90-91	-0.3547157894491093
1102	92-93	-0.2092547193650489
1102	94-95	-0.2035743061514097
1102	96-97	-0.3006430279640071
1102	98-99	-0.335693004367279
1102	100-101	-0.27166822250316613
2203	1	-0.0918674699012314
2203	2	0.05204348129252878
2203	3	-0.17675791983525357
2203	4	-0.12080271999774084
2203	5	-0.15548846517862103
2203	6	-0.11342735722388397
2203	7	0.03422937099344381
2203	8	0.04479345784210409
2203	9	0.04729181588714937
2203	10-11	0.07124282400473447
2203	12-13	0.12887687548938942
2203	14-15	0.051405489790148806
2203	16-17	0.03474583800215214
2203	18-19	0.03980152943957194
2203	20-21	0.08823309072908359
2203	22-23	0.03544403269849994
2203	24-25	-0.009106122790342397
2203	26-27	-0.008238133872197295
2203	28-29	-0.03836345310906353
2203	30-31	-0.011091834738039097
2203	32-33	-0.007618043702500188
2203	34-35	-0.02002386666123357
2203	36-37	-0.04004916742312048
2203	38-39	-0.039705320493141016
2203	40-41	-0.00979861024216433
2203	42-43	-0.0767063056693118
2203	44-45	-0.11937263495301664
2203	46-47	-0.22515399271008363
2203	48-49	-0.17562475007546396
2203	50-51	-0.16927408156792723
2203	52-53	-0.1158575283690979
2203	54-55	-0.1513215046204479
2203	56-57	-0.26876692080892184
2203	58-59	-0.1340542193163472
2203	60-61	-0.20233538007843777
2203	62-63	-0.2144316396407291
2203	64-65	-0.27369897339843163
2203	66-67	-0.27601426149040975
2203	68-69	-0.18894759088244228
2203	70-71	-0.17541378295163668
2203	72-73	-0.22378439555696872
2203	74-75	-0.17529986816028043
2203	76-77	-0.2508404401147999
2203	78-79	-0.2481454882249423
2203	80-81	-0.18222940708854196
2203	82-83	-0.33194783001280115
2203	84-85	-0.2645622215591601
2203	86-87	-0.36510394324809425
2203	88-89	-0.35429418724921646
2203	90-91	-0.24573452075983937
2203	92-93	-0.2843674088748074
2203	94-95	-0.2193513849210973
2203	96-97	-0.30540067613682
2203	98-99	-0.3662913086539987
2203	100-101	-0.23568999400557544
1103	1	-0.12475090352738505
1103	2	0.07257030266872988
1103	3	-0.21781439611383746
1103	4	-0.026231955473996038
1103	5	0.003568709300580508
1103	6	0.003364001891931423
1103	7	0.10905623224823557
1103	8	0.1281616542554147
1103	9	0.11801309541004201
1103	10-11	0.14152205285267883
1103	12-13	0.10821473676460158
1103	14-15	0.1284726776872489
1103	16-17	0.10337927000973934
1103	18-19	0.11694914747121743
1103	20-21	0.11690038979926243
1103	22-23	0.09868261209302176
1103	24-25	0.10752475083965152
1103	26-27	0.08641571309837559
1103	28-29	0.09816684648641427
1103	30-31	0.06603372874035074
1103	32-33	0.09976588497526961
1103	34-35	0.11049737233894064
1103	36-37	0.08793184831397127
1103	38-39	0.0871553239386742
1103	40-41	0.03987580112696065
1103	42-43	-0.02519593408217702
1103	44-45	-0.09984959434089546
1103	46-47	-0.1140137226378144
1103	48-49	-0.019436179007719545
1103	50-51	-0.08657443670924891
1103	52-53	-0.0508191985851667
1103	54-55	-0.03077641935593789
1103	56-57	-0.0300001676060333
1103	58-59	-0.0655248534995323
1103	60-61	-0.1559799957008181
1103	62-63	-0.15337125935614893
1103	64-65	-0.10527377014522443
1103	66-67	-0.18435932306861957
1103	68-69	-0.10628787485546809
1103	70-71	-0.10036675918455984
1103	72-73	-0.1039298628598857
1103	74-75	-0.15391585157730248
1103	76-77	-0.27823460457638305
1103	78-79	-0.16732268378887483
1103	80-81	-0.17035963829059853
1103	82-83	-0.2602448407530673
1103	84-85	-0.17057397729912083
1103	86-87	-0.29180761856711257
1103	88-89	-0.2333030193008696
1103	90-91	-0.22433391898807287
1103	92-93	-0.14523578398971893
1103	94-95	-0.16583507861223268
1103	96-97	-0.22119559694561275
1103	98-99	-0.14650104675529718
1103	100-101	-0.07253500939895474
2204	1	-0.0580291333551628
2204	2	-0.02487607548642501
2204	3	-0.09106585780693166
2204	4	-0.0016271041288007382
2204	5	-0.024272584159149346
2204	6	-0.01161344798997277
2204	7	0.03250399133012394
2204	8	0.03187180661355171
2204	9	0.05577309658894336
2204	10-11	0.077278170480227
2204	12-13	0.08484672661845849
2204	14-15	0.05313160918496607
2204	16-17	0.0747862330036071
2204	18-19	0.11419568293079152
2204	20-21	0.10066732566105685
2204	22-23	0.06931144576653026
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1213	22-23	-0.18956998209306164
1213	24-25	-0.25269067001538303
1213	26-27	-0.18985432041611716
1213	28-29	-0.21817055537081131
1213	30-31	-0.3921523413562866
1213	32-33	-0.2928521197663372
1213	34-35	-0.3766744065054368
1213	36-37	-0.36748746776216024
1213	38-39	-0.5230956431406923
1213	40-41	-0.33333370223266456
1213	42-43	-0.11564312435004354
1213	44-45	-0.20943365924522794
1213	46-47	-0.21798029678419795
1213	48-49	-0.16869498198784783
1213	50-51	-0.294918392994461
1213	52-53	-0.2997607296572298
1213	54-55	-0.10204571823794595
1213	56-57	-0.14542387581833083
1213	58-59	-0.23989527655689358
1213	60-61	-0.12590789277517445
1213	62-63	-0.3110168280015131
1213	64-65	-0.33260859994654624
1213	66-67	-0.14782059491444244
1213	68-69	-0.15675337825209823
1213	70-71	-0.19926004533996888
1213	72-73	-0.10911674562274243
1213	74-75	-0.14364234794218333
1213	76-77	-0.12149378668947008
1213	78-79	-0.1565653974122796
1213	80-81	-0.08033732881542477
1213	82-83	0.008148996052163682
1213	84-85	0.0572330345758445
1213	86-87	-0.009683011441396161
1213	88-89	-0.023201024107031287
1213	90-91	-0.12256624641072023
1213	92-93	-0.12630659333240146
1213	94-95	-0.21420777564522098
1213	96-97	-0.13040632415800957
1213	98-99	-0.09216683583024121
1213	100-101	-0.7522609125476549
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47.0
3	50.0
4	101.0
5	127.0
6	116.0
7	106.0
8	67.0
9	57.0
10	75.0
11	84.0
12	87.0
13	144.0
14	815.0
15	5657.0
16	24233.0
17	48097.0
18	55478.0
19	51088.0
20	48504.0
21	50180.0
22	55923.0
23	63263.0
24	71248.0
25	81508.0
26	92574.0
27	105850.0
28	121527.0
29	140673.0
30	164438.0
31	195384.0
32	233581.0
33	286244.0
34	362309.0
35	487968.0
36	718232.0
37	1252857.0
38	3157604.0
39	5914327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.851012663147738	34.794991765386314	34.64949376994479	15.704501801521156
2	15.756606848284882	37.24113936639489	32.54956627268025	14.452687512639972
3	15.800947308493951	34.58797069476904	33.590562272890665	16.020519723846355
4	16.26695281727363	33.7074242986447	34.18078958800853	15.844833296073132
5	16.686584051399834	34.0166595344082	33.97270567718347	15.324050737008502
6	16.41367435604611	34.254917356957215	33.20583817586485	16.12557011113183
7	16.653905031386515	33.95288788143123	33.885318177374494	15.507888909807754
8	16.047076491103283	34.50082284797136	33.83533598471221	15.61676467621315
9	16.24495740365288	33.89256350148985	33.94594172832186	15.916537366535396
10-11	16.07937603353266	34.34248531675216	33.66971531343195	15.908423336283223
12-13	15.967984294921303	34.253972501608146	33.97890155825187	15.799141645218675
14-15	16.075932577831743	34.21793747042503	33.797915347161116	15.90821460458211
16-17	16.186232904012993	34.00070391401138	33.99266104734514	15.820402134630482
18-19	16.043226472947243	33.90017363103773	33.886541786788456	16.170058109226577
20-21	16.298485930107923	33.90633774485881	33.62307018877952	16.172106136253745
22-23	16.19388728630088	33.958400685430064	33.60584623332258	16.241865794946477
24-25	16.477865307238854	33.69182018654271	33.59262875393206	16.237685752286378
26-27	16.497188942226256	33.78981706284199	33.46982671789191	16.24316727703984
28-29	16.43363920941158	33.76412540255514	33.418696039242256	16.38353934879103
30-31	16.410530413742492	33.638580693709535	33.492810541526396	16.458078351021577
32-33	16.47131205929965	33.79490352287529	33.3730527649309	16.36073165289417
34-35	16.5853534003001	33.78081558116375	33.27214984182298	16.36168117671317
36-37	16.515173648969757	33.800656924691864	33.27748830318923	16.406681123149152
38-39	16.55089710125107	33.72442531289951	33.31295493043653	16.411722655412888
40-41	16.614062564432846	33.85724903509176	33.228016324057855	16.300672076417534
42-43	16.53545531905139	33.709376491989474	33.352247508546476	16.40292068041267
44-45	16.581078382640012	33.76588909095907	33.245450573917445	16.40758195248347
46-47	16.644785122218046	33.83851830011208	33.20414753822198	16.31254903944789
48-49	16.556864011658064	33.69773001903131	33.26806666759262	16.477339301717997
50-51	16.66765561651823	33.73370327311693	33.13663749458043	16.462003615784422
52-53	16.62380821871804	33.85440211839507	33.207991186555034	16.313798476331858
54-55	16.649147528263732	33.66498380662934	33.218984732835224	16.46688393227171
56-57	16.692177380495572	33.80599868234235	33.088801110322585	16.413022826839498
58-59	16.661905598258496	33.78330718523375	33.19032116195842	16.364466054549332
60-61	16.713969666672433	33.68456694878731	33.08915301679983	16.512310367740433
62-63	16.626208301814206	33.73399650863907	33.1534473217873	16.48634786775943
64-65	16.62787754584092	33.76406284697096	33.16231013939386	16.445749467794258
66-67	16.71528139120313	33.717170373527324	33.1024242713485	16.465123963921048
68-69	16.636661354175377	33.736918748766506	33.14432077805452	16.4820991190036
70-71	16.74958653701713	33.717631529681384	33.07237105471159	16.46041087858989
72-73	16.694260018269027	33.715414840564925	33.10905143781086	16.48127370335518
74-75	16.71422885157135	33.74468744871508	33.082296150083366	16.458787549630205
76-77	16.702532648234694	33.79073068387131	33.098574181251706	16.40816248664229
78-79	16.730517132922323	33.68106050142987	33.13546507376836	16.452957291879443
80-81	16.81276234191477	33.70517597367445	33.06444010602036	16.417621578390417
82-83	16.705089257713844	33.86289986716585	33.07416129537488	16.35784957974543
84-85	16.658344929657233	33.66081983177601	33.14490236507696	16.535932873489802
86-87	16.715585646447305	33.868714313738465	33.01290784920737	16.40279219060686
88-89	16.6874393736404	33.800859804705	33.143348219381984	16.36835260227262
90-91	16.703011991617522	33.68191400114635	33.069881872162036	16.545192135074092
92-93	16.737146595095233	33.787129830742614	33.081594170046294	16.394129404115866
94-95	16.69621751552898	33.81037416532969	33.064196791749254	16.429211527392074
96-97	16.67572279927181	33.746393099999615	33.12055684060862	16.457327260119953
98-99	16.699375266943896	33.81398409832401	33.05269112457391	16.43394951015818
100-101	16.80376343227856	33.7870398881392	32.92731166368857	16.48188501589367
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1427.0
1	1597.5
2	1895.5
3	2282.0
4	2809.0
5	3330.0
6	3902.0
7	4653.5
8	5895.0
9	7727.5
10	10207.5
11	14346.5
12	20951.0
13	30950.5
14	45971.5
15	67282.5
16	96523.5
17	134034.0
18	178714.5
19	230410.0
20	286116.0
21	342291.5
22	397246.5
23	445701.0
24	486360.5
25	517133.5
26	533160.5
27	540814.5
28	549919.5
29	556679.0
30	559293.0
31	560003.0
32	554025.5
33	533817.0
34	500652.5
35	461902.0
36	427820.0
37	402849.0
38	382285.5
39	365483.5
40	348058.0
41	325691.0
42	302155.0
43	277489.0
44	250444.0
45	225927.0
46	203362.5
47	191023.0
48	179874.5
49	160013.0
50	138193.0
51	119207.5
52	104691.0
53	92923.0
54	84836.0
55	77198.5
56	69977.0
57	63355.0
58	56788.5
59	49969.0
60	42613.0
61	34837.0
62	27693.5
63	22015.5
64	17520.0
65	13747.5
66	10655.0
67	8191.0
68	6247.0
69	4575.5
70	3325.5
71	2518.0
72	1873.5
73	1400.5
74	1066.0
75	830.5
76	642.0
77	481.5
78	353.5
79	269.0
80	210.5
81	144.0
82	92.5
83	81.0
84	65.0
85	39.0
86	28.5
87	23.5
88	17.5
89	14.0
90	10.0
91	6.5
92	6.0
93	4.0
94	4.0
95	6.0
96	5.0
97	5.5
98	8.5
99	14.5
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.98821721107161E-4
2	3.335599849259892E-4
3	0.0013342399397039569
4	0.001566281668348123
5	0.008426015271391292
6	0.013516430693522692
7	0.013770226334227252
8	0.009571721306571863
9	0.002581464231166351
10-11	0.006116474940979824
12-13	0.01053614474124918
14-15	0.03395423107425966
16-17	0.014553367168401311
18-19	0.022141856825467564
20-21	0.017562658336755344
22-23	0.03076003165339231
24-25	0.02643825445739471
26-27	0.02189531248878314
28-29	0.030147296463691305
30-31	0.03289191503531059
32-33	0.03755450352025431
34-35	0.018780877412137218
36-37	0.02870791261569546
38-39	0.0287768000038867
40-41	0.03239520070993167
42-43	0.04022660905167228
44-45	0.028164064814185697
46-47	0.02821119829031654
48-49	0.022366647250091604
50-51	0.035937462723765266
52-53	0.028711538267705526
54-55	0.03097394512198615
56-57	0.023773400229996862
58-59	0.025027875825479384
60-61	0.032627242438575836
62-63	0.029164744768963663
64-65	0.02825470611443732
66-67	0.02396555978653031
68-69	0.026249720552871322
70-71	0.029150242160923403
72-73	0.03622388923256042
74-75	0.03107546337826797
76-77	0.03320372110817619
78-79	0.03456696626396066
80-81	0.04013959340343072
82-83	0.041796516372030475
84-85	0.04670927484566868
86-87	0.04790936566100023
88-89	0.05011738773512987
90-91	0.049812832966284415
92-93	0.05011738773512987
94-95	0.05102742638965622
96-97	0.05181419287584035
98-99	0.05290551413086994
100-101	0.056407893972592826
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.3790623E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.91256692456277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.411064384009386	20.774430402681197
2	23.28188086397231	19.98170541409818
3	13.13047558655757	16.90387237078468
4	6.69325222881317	11.488985368476964
5	3.3475994800060302	7.182704336119515
6	1.7325225699077695	4.460819443768958
7	1.031910063043554	3.0997336748340407
8	0.6300435680744623	2.1629429424308544
9	0.32705408477372505	1.2631257270723688
>10	1.3242388126201268	9.816891269540475
>50	0.07816742915770124	2.2008596018046442
>100	0.011739170054187986	0.6488258869104502
>500	5.175901003382153E-5	0.015103561477773279
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.35078241207812E-5	0.0	0.0
2	4.35078241207812E-5	0.0	7.2513040201302E-6
3	4.35078241207812E-5	0.0	7.2513040201302E-6
4	4.35078241207812E-5	0.0	7.2513040201302E-6
5	5.07591281409114E-5	0.0	7.2513040201302E-6
6	5.07591281409114E-5	0.0	7.2513040201302E-6
7	5.07591281409114E-5	0.0	7.2513040201302E-6
8	5.07591281409114E-5	0.0	7.2513040201302E-6
9	5.07591281409114E-5	0.0	3.6256520100651E-5
10-11	5.07591281409114E-5	2.17539120603906E-5	4.35078241207812E-5
12-13	5.801043216104161E-5	2.17539120603906E-5	4.35078241207812E-5
14-15	9.06413002516275E-5	2.17539120603906E-5	5.80104321610416E-5
16-17	1.377747763824738E-4	2.17539120603906E-5	1.08769560301953E-4
18-19	2.030365125636456E-4	2.90052160805208E-5	1.2327216834221341E-4
20-21	2.8642650879514295E-4	5.07591281409114E-5	1.45026080402604E-4
22-23	3.661908530165751E-4	5.07591281409114E-5	1.595286884428644E-4
24-25	4.205756331675516E-4	5.07591281409114E-5	1.776569484931899E-4
26-27	4.640834572883328E-4	5.07591281409114E-5	1.921595565334503E-4
28-29	5.510991055298953E-4	5.07591281409114E-5	2.030365125636456E-4
30-31	6.381147537714576E-4	6.52617361811718E-5	2.102878165837758E-4
32-33	8.629051783954939E-4	6.52617361811718E-5	2.247904246240362E-4
34-35	0.0010695673429692047	6.52617361811718E-5	2.392930326642966E-4
36-37	0.0012109677713617434	6.52617361811718E-5	2.392930326642966E-4
38-39	0.0014212555879455193	6.52617361811718E-5	2.392930326642966E-4
40-41	0.0015807842763883835	7.2513040201302E-5	2.4654433668442683E-4
42-43	0.0018019490490023546	7.2513040201302E-5	2.5379564070455703E-4
44-45	0.001954226433425089	7.2513040201302E-5	2.5742129271462215E-4
46-47	0.0021645142500088645	7.97643442214322E-5	2.755495527649476E-4
48-49	0.002385679022622836	8.70156482415624E-5	2.755495527649476E-4
50-51	0.0026576029233777183	8.70156482415624E-5	2.755495527649476E-4
52-53	0.002998414212323838	8.70156482415624E-5	2.755495527649476E-4
54-55	0.0033174715892095663	1.051439082918879E-4	2.973034648253382E-4
56-57	0.003654657226145621	1.341491243724087E-4	3.045547688454684E-4
58-59	0.0040679815552930425	1.377747763824738E-4	3.190573768857288E-4
60-61	0.004361659368108315	1.377747763824738E-4	3.4443694095618455E-4
62-63	0.004807614565346323	1.740312964831248E-4	3.698165050266402E-4
64-65	0.0052463184585641994	1.740312964831248E-4	4.060730251272912E-4
66-67	0.005674145395751881	1.740312964831248E-4	4.423295452279422E-4
68-69	0.006127351897010019	1.740312964831248E-4	4.71334761308463E-4
70-71	0.0065696814422379615	1.994108605535805E-4	4.71334761308463E-4
72-73	0.007109903591737661	2.030365125636456E-4	5.075912814091141E-4
74-75	0.007541356180935408	2.247904246240362E-4	5.43847801509765E-4
76-77	0.008041696158324393	2.247904246240362E-4	5.474734535198301E-4
78-79	0.008766826560337412	2.247904246240362E-4	5.510991055298953E-4
80-81	0.009517336526420887	2.247904246240362E-4	5.510991055298953E-4
82-83	0.010347610836725796	2.247904246240362E-4	6.16360841711067E-4
84-85	0.01122501862316155	2.247904246240362E-4	6.743712738721086E-4
86-87	0.012367099006332056	2.247904246240362E-4	6.816225778922388E-4
88-89	0.01395513458674057	2.247904246240362E-4	6.961251859324992E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	1130	0.0	22.069801	32-33
CCGATCT	920	0.0	20.136982	32-33
GACCGGA	490	2.910383E-11	15.509908	9
GTGTGAC	2680	0.0	12.75771	5
GTGACTG	2165	0.0	11.405454	7
GACGGGA	925	5.456968E-12	10.783577	9
CAGACGT	1695	0.0	9.94853	18-19
CCGTGTG	1345	0.0	9.179616	3
TTCCGAT	2875	0.0	8.921844	30-31
TGACTGG	3005	0.0	8.692921	8
CTGTGTG	4220	0.0	8.552136	3
CGATCTA	1480	0.0	8.184425	34-35
AGACGCG	320	3.5463893E-4	8.164158	20-21
CTTCCGA	3335	0.0	8.04732	30-31
AACCGTG	865	1.8426523E-5	7.685707	1
TCAGACG	2330	0.0	7.237235	18-19
CGTGTGC	2255	0.0	7.162232	22-23
CACTGTG	4610	0.0	7.0045447	1
ACCGTGT	1810	1.8735591E-10	6.821317	2
AACTGTG	3345	0.0	6.6722703	1
>>END_MODULE