FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 3-58925.34200000001ae4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 3-58925.34200000001ae4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13790623
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC11300.022.06980132-33
CCGATCT9200.020.13698232-33
GACCGGA4902.910383E-1115.5099089
GTGTGAC26800.012.757715
GTGACTG21650.011.4054547
GACGGGA9255.456968E-1210.7835779
CAGACGT16950.09.9485318-19
CCGTGTG13450.09.1796163
TTCCGAT28750.08.92184430-31
TGACTGG30050.08.6929218
CTGTGTG42200.08.5521363
CGATCTA14800.08.18442534-35
AGACGCG3203.5463893E-48.16415820-21
CTTCCGA33350.08.0473230-31
AACCGTG8651.8426523E-57.6857071
TCAGACG23300.07.23723518-19
CGTGTGC22550.07.16223222-23
CACTGTG46100.07.00454471
ACCGTGT18101.8735591E-106.8213172
AACTGTG33450.06.67227031
Produced by FastQC (version 0.11.2)