##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l02n02 2-58885.34200000001aca.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12133707
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.31405142715248	38.0	32.0	38.0	32.0	38.0
2	35.206212413073764	38.0	32.0	38.0	32.0	38.0
3	35.08798547715055	38.0	32.0	38.0	32.0	38.0
4	35.49937673622744	38.0	32.0	38.0	32.0	38.0
5	35.40015924234861	38.0	32.0	38.0	32.0	38.0
6	37.52120658591805	40.0	38.0	40.0	32.0	40.0
7	37.63598288635122	40.0	38.0	40.0	32.0	40.0
8	37.737747087514144	40.0	38.0	40.0	32.0	40.0
9	37.736344301045015	40.0	38.0	40.0	32.0	40.0
10-11	37.754457067407344	40.0	38.0	40.0	32.0	40.0
12-13	37.73254294833393	40.0	38.0	40.0	32.0	40.0
14-15	37.69529188400544	40.0	38.0	40.0	32.0	40.0
16-17	37.68825528752261	40.0	38.0	40.0	32.0	40.0
18-19	37.67226371957061	40.0	38.0	40.0	32.0	40.0
20-21	37.65640611727315	40.0	38.0	40.0	32.0	40.0
22-23	37.64596615032818	40.0	38.0	40.0	32.0	40.0
24-25	37.58949845253392	40.0	38.0	40.0	32.0	40.0
26-27	37.570081632925536	40.0	38.0	40.0	32.0	40.0
28-29	37.50222804951529	40.0	38.0	40.0	32.0	40.0
30-31	37.466522514512675	40.0	38.0	40.0	32.0	40.0
32-33	37.46589294598922	40.0	38.0	40.0	32.0	40.0
34-35	37.391951033595916	40.0	38.0	40.0	32.0	40.0
36-37	37.39112552330462	40.0	38.0	40.0	32.0	40.0
38-39	37.34724124292765	40.0	38.0	40.0	32.0	40.0
40-41	37.2581311300825	40.0	38.0	40.0	32.0	40.0
42-43	37.048600728532506	39.0	38.0	40.0	32.0	40.0
44-45	36.958210668841765	38.0	38.0	40.0	32.0	40.0
46-47	36.88235091715994	38.0	38.0	40.0	32.0	40.0
48-49	36.80320020913642	38.0	38.0	40.0	32.0	40.0
50-51	36.73727357187708	38.0	38.0	40.0	32.0	40.0
52-53	36.66964217942629	38.0	38.0	40.0	32.0	40.0
54-55	36.61751338646961	38.0	38.0	40.0	32.0	40.0
56-57	36.590619379551526	38.0	38.0	40.0	32.0	40.0
58-59	36.520819317624856	38.0	38.0	40.0	32.0	40.0
60-61	36.48229976214194	38.0	38.0	40.0	32.0	40.0
62-63	36.406399008975576	38.0	38.0	40.0	27.0	40.0
64-65	36.385604127411355	38.0	38.0	40.0	27.0	40.0
66-67	36.31876470232881	38.0	38.0	40.0	27.0	40.0
68-69	36.26473986886283	38.0	38.0	40.0	27.0	40.0
70-71	36.15003732989432	38.0	38.0	40.0	27.0	40.0
72-73	36.08072718419854	38.0	38.0	40.0	27.0	40.0
74-75	35.989391906364645	38.0	38.0	40.0	27.0	40.0
76-77	35.90704658518621	38.0	38.0	40.0	27.0	40.0
78-79	35.817626839019596	38.0	38.0	40.0	27.0	40.0
80-81	35.73777947662656	38.0	38.0	40.0	27.0	40.0
82-83	35.64698319318243	38.0	38.0	40.0	27.0	40.0
84-85	35.543887041280954	38.0	38.0	40.0	27.0	40.0
86-87	35.47555953015843	38.0	38.0	40.0	27.0	40.0
88-89	35.40256971756447	38.0	38.0	40.0	27.0	40.0
90-91	35.28245951546382	38.0	38.0	40.0	27.0	40.0
92-93	35.17778701100991	38.0	38.0	40.0	27.0	40.0
94-95	35.074486263760946	38.0	38.0	40.0	27.0	40.0
96-97	34.988634429692425	38.0	38.0	40.0	27.0	40.0
98-99	34.848376839823146	38.0	32.0	40.0	27.0	40.0
100-101	32.903253556394596	38.0	29.5	39.0	13.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.19909052348301515
2201	2	0.2551360536723237
2201	3	0.32566601767849335
2201	4	0.2286652192903489
2201	5	0.35657397590413353
2201	6	0.37444063037962394
2201	7	0.3218199782904989
2201	8	0.2320880053613692
2201	9	0.28205019527595
2201	10-11	0.211458128409987
2201	12-13	0.29927231187730996
2201	14-15	0.29451541896067823
2201	16-17	0.22511339461407687
2201	18-19	0.24900501365085148
2201	20-21	0.31942259961100916
2201	22-23	0.29092428444383245
2201	24-25	0.2683657356724609
2201	26-27	0.29506673373094827
2201	28-29	0.3240744762711856
2201	30-31	0.2871065599025684
2201	32-33	0.20117095570381593
2201	34-35	0.26932671401966957
2201	36-37	0.2008173031881455
2201	38-39	0.2868697956243125
2201	40-41	0.2794596811639991
2201	42-43	0.2790618418368709
2201	44-45	0.20443892136541564
2201	46-47	0.25886171484101084
2201	48-49	0.1968240366883478
2201	50-51	0.23968167013811836
2201	52-53	0.24886437934759442
2201	54-55	0.29522622408978805
2201	56-57	0.14851849183105514
2201	58-59	0.16832991527702035
2201	60-61	0.13557649500143754
2201	62-63	0.20470800792144672
2201	64-65	0.20818459180463833
2201	66-67	0.2427103043506662
2201	68-69	0.1583910632181187
2201	70-71	0.23532082477009197
2201	72-73	0.08535921551176529
2201	74-75	0.16660334634151042
2201	76-77	0.14430058811208823
2201	78-79	0.17364222471251622
2201	80-81	0.25504482061874967
2201	82-83	0.20652326774871455
2201	84-85	0.1457807501755184
2201	86-87	0.2452877592195577
2201	88-89	0.16958271939602554
2201	90-91	0.07069921379549271
2201	92-93	0.025628225960510065
2201	94-95	0.19215864207382793
2201	96-97	-0.021918741757197324
2201	98-99	0.01485152187036931
2201	100-101	0.1951033639367452
1101	1	0.1467122008566477
1101	2	0.1734856079451319
1101	3	0.11524751148363777
1101	4	0.14913858065305874
1101	5	0.2350783450583549
1101	6	0.2811291005076768
1101	7	0.32097411441969825
1101	8	0.26051256274713097
1101	9	0.27827257375268744
1101	10-11	0.3292805293321521
1101	12-13	0.3228267582716171
1101	14-15	0.2804847885058521
1101	16-17	0.27181691755460236
1101	18-19	0.30494416175110217
1101	20-21	0.33065875451703164
1101	22-23	0.3087155061609579
1101	24-25	0.24329215690860906
1101	26-27	0.235881731346943
1101	28-29	0.27112565763001584
1101	30-31	0.29527891058724265
1101	32-33	0.26056896044269706
1101	34-35	0.2668001016664192
1101	36-37	0.2830265092957589
1101	38-39	0.2736979110558764
1101	40-41	0.2386889764085538
1101	42-43	0.2776591912522477
1101	44-45	0.2535672296442897
1101	46-47	0.1933769104665899
1101	48-49	0.12427850502700721
1101	50-51	0.20486461934156353
1101	52-53	0.23354650462204773
1101	54-55	0.2057252298818213
1101	56-57	0.20819674540982192
1101	58-59	0.18389181556009504
1101	60-61	0.1306236081961103
1101	62-63	0.19552868544266744
1101	64-65	0.18413597238324542
1101	66-67	0.18029073421950415
1101	68-69	0.1422500631520336
1101	70-71	0.15235367159342417
1101	72-73	0.19399372564203787
1101	74-75	0.17986246224665337
1101	76-77	0.24610958001424166
1101	78-79	0.17158051130137864
1101	80-81	0.22704846615824437
1101	82-83	0.2171752291573199
1101	84-85	0.18852176512329066
1101	86-87	0.20601660380629028
1101	88-89	0.15398421764297865
1101	90-91	0.1443364194579857
1101	92-93	0.17635453290835557
1101	94-95	0.26053564804001184
1101	96-97	0.19250752494203027
1101	98-99	0.09010052768604027
1101	100-101	0.22132409209729076
2202	1	0.04974559195868267
2202	2	0.011073787290833081
2202	3	0.04727212763701516
2202	4	0.12944044937631816
2202	5	0.14034627909313002
2202	6	0.17886767711149787
2202	7	0.3090673289211239
2202	8	0.1888164822877485
2202	9	0.28767518790572666
2202	10-11	0.23009539903317489
2202	12-13	0.2684933360621784
2202	14-15	0.2698660188279902
2202	16-17	0.2431536061104751
2202	18-19	0.2785509498137415
2202	20-21	0.24888256731865255
2202	22-23	0.25602990509752743
2202	24-25	0.28863655152429146
2202	26-27	0.22223179055400522
2202	28-29	0.2490517724791914
2202	30-31	0.23677135732107502
2202	32-33	0.26361578717862244
2202	34-35	0.24013899891390622
2202	36-37	0.26387858726562285
2202	38-39	0.22872397341159
2202	40-41	0.18912136120742673
2202	42-43	0.11116403141388531
2202	44-45	0.107199353221894
2202	46-47	0.059367031700872985
2202	48-49	0.10950279443997601
2202	50-51	0.24720776898671915
2202	52-53	0.21666149606908647
2202	54-55	0.16707691787117085
2202	56-57	0.1611039769594953
2202	58-59	0.1420049874147864
2202	60-61	0.06307473848212908
2202	62-63	0.09726638809042498
2202	64-65	0.061599765177561494
2202	66-67	0.09484862592371712
2202	68-69	0.09651430100430503
2202	70-71	0.11328723841084098
2202	72-73	0.04441736076501712
2202	74-75	0.10330169072049245
2202	76-77	-0.002287739979493608
2202	78-79	0.018581289760469133
2202	80-81	0.05749277804206088
2202	82-83	0.06181119658448608
2202	84-85	0.08005770812217605
2202	86-87	0.06847507598842384
2202	88-89	0.09288453074160685
2202	90-91	0.16549963292995074
2202	92-93	0.0558086988062243
2202	94-95	0.1503419667834649
2202	96-97	0.0383815489044963
2202	98-99	0.014447349206498927
2202	100-101	0.13424866498658616
1102	1	-0.01236889120441731
1102	2	-0.12733479467157593
1102	3	-0.17121619710335523
1102	4	0.07392515306005976
1102	5	0.057404165015405795
1102	6	0.012484231561700199
1102	7	0.17695732652430252
1102	8	0.2408428543403005
1102	9	0.2235237472099456
1102	10-11	0.15853499404649085
1102	12-13	0.2958205307274966
1102	14-15	0.17496948492748743
1102	16-17	0.24639776710373695
1102	18-19	0.22677190405659076
1102	20-21	0.2543948239247342
1102	22-23	0.27504776538537357
1102	24-25	0.2510598537509381
1102	26-27	0.18844042433217112
1102	28-29	0.06549235926718211
1102	30-31	0.1721216963785963
1102	32-33	0.1558849020896247
1102	34-35	0.24349261978082382
1102	36-37	0.19465039467363
1102	38-39	0.1834664417494949
1102	40-41	0.21614129033908114
1102	42-43	0.13901916619041543
1102	44-45	0.034227071330249714
1102	46-47	0.06488036777074768
1102	48-49	0.012059775517172966
1102	50-51	0.05775475285974352
1102	52-53	0.12883689241994745
1102	54-55	0.08224640255446047
1102	56-57	-0.006033800508447484
1102	58-59	0.030651109407244803
1102	60-61	0.04651283418197494
1102	62-63	-0.04292301728336412
1102	64-65	-0.03807548010448869
1102	66-67	-0.11803507790042289
1102	68-69	-0.18468997402253962
1102	70-71	-0.0438624962981109
1102	72-73	0.021838420513198287
1102	74-75	0.07572623060756456
1102	76-77	-0.017665279803459555
1102	78-79	-0.01735078225079434
1102	80-81	0.0034438144923143454
1102	82-83	-0.005953007379886799
1102	84-85	0.07508630777640946
1102	86-87	0.08258188674942346
1102	88-89	-0.11958701809123085
1102	90-91	-0.19949380037562037
1102	92-93	-0.016421007407281252
1102	94-95	-0.06192632294575873
1102	96-97	-0.06538330014893745
1102	98-99	-0.10169465789617504
1102	100-101	-0.03864720059674198
2203	1	-0.03925697146183893
2203	2	-0.23795704593980815
2203	3	-0.1709902536818504
2203	4	-0.081369965783054
2203	5	-0.10780942536832328
2203	6	-0.09770616630787288
2203	7	0.08962923037399406
2203	8	0.1252397857030303
2203	9	0.10045514783969622
2203	10-11	0.09968581658182529
2203	12-13	0.13327189118349736
2203	14-15	0.09953515284836811
2203	16-17	0.15269583044572954
2203	18-19	0.16904059932021198
2203	20-21	0.193020454213233
2203	22-23	0.07128280603061654
2203	24-25	0.1067854978105629
2203	26-27	0.09249275271969282
2203	28-29	0.09203806327817432
2203	30-31	0.10031785165462281
2203	32-33	0.0799964344415045
2203	34-35	0.09163504433549008
2203	36-37	0.04016994805943597
2203	38-39	0.031777491714620965
2203	40-41	0.09259873067500735
2203	42-43	0.023200749823622857
2203	44-45	-0.07661120101483476
2203	46-47	-0.143395015040646
2203	48-49	-0.14962317167161387
2203	50-51	-0.08554626408493249
2203	52-53	-0.09863586784914702
2203	54-55	-0.03978583541373126
2203	56-57	-0.17674068365943185
2203	58-59	-0.035610827235082354
2203	60-61	-0.12095103579698474
2203	62-63	-0.15409726816776725
2203	64-65	-0.21395876715254758
2203	66-67	-0.22599911361720615
2203	68-69	-0.11343821240254215
2203	70-71	-0.09988832295429262
2203	72-73	-0.16845506026280788
2203	74-75	-0.05707722701610152
2203	76-77	-0.14859590077322338
2203	78-79	-0.1352896655589504
2203	80-81	-0.15363098312906942
2203	82-83	-0.21929378978596503
2203	84-85	-0.20970099152950894
2203	86-87	-0.3358972875541042
2203	88-89	-0.3231443033351198
2203	90-91	-0.14366146397748025
2203	92-93	-0.24497464986025363
2203	94-95	-0.152798291478895
2203	96-97	-0.21230252085106827
2203	98-99	-0.28019096818147915
2203	100-101	-0.12863782281040415
1103	1	-0.1323954708739663
1103	2	-0.1088400798723228
1103	3	-0.16503061208722158
1103	4	-0.02136830472268514
1103	5	-0.010274101593068963
1103	6	-0.06053359186640961
1103	7	0.09846010063959199
1103	8	0.07928856116887317
1103	9	0.09296412765356621
1103	10-11	0.14779936327072107
1103	12-13	0.11705066182579316
1103	14-15	0.1318364162099357
1103	16-17	0.10364238505366785
1103	18-19	0.07229702966665741
1103	20-21	0.10063738911190256
1103	22-23	0.08041308669289293
1103	24-25	0.10934121379138162
1103	26-27	0.027558367302539466
1103	28-29	-0.007770500471586672
1103	30-31	0.0775251161808228
1103	32-33	0.07471867188112213
1103	34-35	0.04243962719791483
1103	36-37	0.085596218677928
1103	38-39	0.11209148748167763
1103	40-41	0.01711932057321519
1103	42-43	-0.05009082512741969
1103	44-45	-0.2006479892371047
1103	46-47	-0.20885361453836282
1103	48-49	-0.15030017823889352
1103	50-51	-0.12724776088276712
1103	52-53	-0.07938603209689887
1103	54-55	-0.06708837590477401
1103	56-57	-0.04502207787015777
1103	58-59	-0.13546738626408938
1103	60-61	-0.15756471442893627
1103	62-63	-0.1757568130076308
1103	64-65	-0.12182582202200365
1103	66-67	-0.19003925250914477
1103	68-69	-0.22833795874006313
1103	70-71	-0.08755204606948297
1103	72-73	-0.12175741347981983
1103	74-75	-0.2661272207727663
1103	76-77	-0.38007490791729737
1103	78-79	-0.25918896978841843
1103	80-81	-0.22416397060951
1103	82-83	-0.31311007389781764
1103	84-85	-0.1977411932290991
1103	86-87	-0.3180607109316398
1103	88-89	-0.25360413626851397
1103	90-91	-0.23924315132131824
1103	92-93	-0.22034638523123107
1103	94-95	-0.2018458895624562
1103	96-97	-0.266187554270644
1103	98-99	-0.23371749665773933
1103	100-101	-0.05198948368711598
2204	1	-0.05542183294795677
2204	2	-0.03964256425349788
2204	3	-0.013330825665441637
2204	4	0.02463406880804797
2204	5	0.03730532049190316
2204	6	0.05549673647200848
2204	7	0.13758724237377606
2204	8	0.17172102486115648
2204	9	0.19731469448519334
2204	10-11	0.1825556891848592
2204	12-13	0.22432614478228885
2204	14-15	0.13913837607805846
2204	16-17	0.17868430408440616
2204	18-19	0.17785931474769967
2204	20-21	0.17362302254523598
2204	22-23	0.15144891883487333
2204	24-25	0.1524565083021301
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1213	24-25	-0.4426957755524654
1213	26-27	-0.3599103458213406
1213	28-29	-0.3781308092128981
1213	30-31	-0.5794814256074119
1213	32-33	-0.45190118277265867
1213	34-35	-0.4931573126431772
1213	36-37	-0.5084880207861531
1213	38-39	-0.7208119828595585
1213	40-41	-0.5415173860132683
1213	42-43	-0.212232617796424
1213	44-45	-0.33094473975342
1213	46-47	-0.3333156247304956
1213	48-49	-0.2557283465108071
1213	50-51	-0.5174057641765373
1213	52-53	-0.49345378487682723
1213	54-55	-0.2824206617801934
1213	56-57	-0.33278708501245546
1213	58-59	-0.4342153735718952
1213	60-61	-0.3275199849207766
1213	62-63	-0.4809697577203096
1213	64-65	-0.4865001667974127
1213	66-67	-0.28804110945315387
1213	68-69	-0.3202322632215413
1213	70-71	-0.3680231513981269
1213	72-73	-0.3185422613473108
1213	74-75	-0.3798906499950263
1213	76-77	-0.3671067309460554
1213	78-79	-0.390803342322009
1213	80-81	-0.29546591367007835
1213	82-83	-0.1771053912393583
1213	84-85	-0.1707752141809209
1213	86-87	-0.24240297019325396
1213	88-89	-0.3308159244863518
1213	90-91	-0.2693772798001248
1213	92-93	-0.33092202330261244
1213	94-95	-0.4518626998400421
1213	96-97	-0.4318037645034778
1213	98-99	-0.32308709893752763
1213	100-101	-1.0057411125517675
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45.0
3	48.0
4	88.0
5	105.0
6	93.0
7	78.0
8	57.0
9	57.0
10	67.0
11	89.0
12	80.0
13	119.0
14	625.0
15	4806.0
16	21900.0
17	43143.0
18	52039.0
19	53428.0
20	56593.0
21	63496.0
22	71850.0
23	81529.0
24	91523.0
25	101661.0
26	114436.0
27	127998.0
28	143911.0
29	164256.0
30	189691.0
31	219684.0
32	256936.0
33	309132.0
34	383187.0
35	502654.0
36	719044.0
37	1228478.0
38	2965990.0
39	4164791.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.57016035005486	30.964522520486636	29.79710706786199	19.668210061596515
2	21.05146605296931	31.944083190672433	28.776121120175695	18.228329636182565
3	21.079235939828642	29.972810094195868	29.560020660075736	19.38793330589975
4	20.96415416603069	29.217944740831925	29.72635466916597	20.09154642397142
5	21.243863893707367	29.493111752426955	29.539548265208037	19.723476088657637
6	21.345243088866443	30.04909196878668	28.260971880693305	20.344693061653572
7	21.43262385041306	30.142296350789128	28.9868303649623	19.438249433835512
8	20.587860756320925	30.04998680826972	29.523347878991483	19.83880455641787
9	20.69297885220278	29.601377683034823	29.68562436873708	20.020019096025315
10-11	20.855190576866388	29.91650904890191	29.126056834375923	20.10224353985577
12-13	20.57975498818848	29.95761650659507	29.51156014800161	19.95106835721484
14-15	20.836094994351228	29.744060956864583	29.338261157505553	20.08158289127864
16-17	20.82636121087412	29.715845680658088	29.422974293531546	20.03481881493625
18-19	20.685388966339527	29.59206081860855	29.435449966960746	20.287100248091175
20-21	21.002124858386736	29.487026147842272	29.136459134269817	20.37438985950118
22-23	20.934513410034334	29.439647683502123	29.120430931485792	20.505407974977746
24-25	21.137042089483927	29.298791908288308	29.06925135047619	20.494914651751582
26-27	21.296737009210602	29.356266720567064	28.87151350364825	20.475482766574082
28-29	21.17974682083355	29.40910434294067	28.856011201844662	20.555137634381122
30-31	21.38377776150923	29.108644007655894	28.909509549418356	20.598068681416525
32-33	21.334987800496357	29.307582325007576	28.875327289177456	20.482102585318614
34-35	21.499315294385497	29.18975087233713	28.677315921019108	20.63361791225827
36-37	21.406346002238536	29.324714715301443	28.720592838515874	20.54834644394415
38-39	21.40306502253945	29.130821812445312	28.834126417134765	20.63198674788047
40-41	21.610107817828215	29.31686713410548	28.577790087163834	20.49523496090247
42-43	21.484496385115822	29.231270386773524	28.697614396579535	20.58661883153112
44-45	21.513069805378684	29.026598295874322	28.69761290834228	20.76271899040471
46-47	21.535142390578578	29.326049213315947	28.507080339920286	20.63172805618519
48-49	21.43241693278133	29.090584004730342	28.655419659091315	20.82157940339702
50-51	21.518911994053465	29.213435532237774	28.455884820204496	20.811767653504262
52-53	21.50590505742654	29.24679809534487	28.60866360875429	20.638633238474295
54-55	21.63400873439342	29.016272741797223	28.561807779041317	20.787910744768045
56-57	21.6451363751828	29.166465561020456	28.454096123236827	20.734301940559916
58-59	21.470305723005538	29.138956564089618	28.59527194494213	20.795465767962718
60-61	21.583741804352634	29.030699105924885	28.39343608702798	20.992123002694505
62-63	21.439057130747972	29.126209190129398	28.534937362135572	20.899796316987057
64-65	21.578615639027863	28.983116162740952	28.47326515891872	20.965003039312467
66-67	21.59184051324719	29.08281614272738	28.311978397401717	21.01336494662372
68-69	21.42681258087564	29.005572479369572	28.503829229891213	21.06378570986357
70-71	21.729406184536774	29.084151993128195	28.20830888150059	20.978132940834445
72-73	21.629400188469088	29.143182918072007	28.283706987454355	20.943709906004546
74-75	21.654351649551117	28.997322815197414	28.368562790181556	20.979762745069912
76-77	21.640997872649848	29.13873234861633	28.301696241043846	20.918573537689976
78-79	21.617466349588337	29.06085949992147	28.41336758043101	20.90830657005918
80-81	21.753154373538344	29.063295240788555	28.20734458781767	20.976205797855435
82-83	21.63722513959477	29.320452252131403	28.348690369040057	20.693632239233775
84-85	21.71164817760711	29.027361763287434	28.233437497397546	21.02755256170791
86-87	21.567308899796576	29.19409632664154	28.30926020703276	20.92933456652912
88-89	21.60805882628201	29.059896364255916	28.30274978772545	21.029295021736623
90-91	21.630232423524454	28.97210841846558	28.257766869328332	21.139892288681637
92-93	21.491022307383822	29.100316086583653	28.39071578581346	21.01794582021906
94-95	21.61291030120804	28.931475352737497	28.299626829488716	21.155987516565748
96-97	21.656338430007704	29.07379825893574	28.248589294324546	21.02127401673201
98-99	21.535255203892774	29.111611171766793	28.263488454523554	21.08964516981688
100-101	21.93856817625572	28.875920857239173	27.799391163860637	21.38611980264447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	317.0
1	323.5
2	391.0
3	488.0
4	579.0
5	763.0
6	973.5
7	1184.5
8	1395.0
9	1633.5
10	2055.0
11	2615.5
12	3391.5
13	4661.5
14	6555.5
15	9057.0
16	11978.5
17	15934.5
18	21485.0
19	27542.0
20	34097.0
21	41711.0
22	49846.5
23	58479.5
24	67770.0
25	78315.5
26	88601.0
27	99573.0
28	112227.0
29	128203.5
30	148103.0
31	171113.5
32	205848.0
33	255496.5
34	313716.5
35	386379.5
36	477136.0
37	566468.0
38	636163.0
39	677591.0
40	682138.0
41	653670.5
42	601728.5
43	535021.0
44	468516.0
45	413946.0
46	374687.0
47	351408.0
48	335798.5
49	319170.5
50	302763.5
51	283386.0
52	260646.0
53	239244.0
54	220280.5
55	204381.0
56	190569.0
57	173762.0
58	154410.0
59	135194.0
60	114516.5
61	92849.5
62	73965.5
63	58487.0
64	45809.5
65	35586.5
66	27028.5
67	20106.0
68	14396.5
69	10437.0
70	7593.5
71	5465.0
72	4092.0
73	3089.0
74	2340.0
75	1752.5
76	1282.0
77	991.5
78	787.0
79	622.0
80	479.5
81	339.5
82	233.0
83	162.0
84	133.0
85	91.5
86	55.0
87	49.5
88	49.0
89	36.5
90	24.5
91	14.0
92	9.0
93	10.0
94	7.5
95	6.0
96	9.0
97	8.5
98	5.0
99	11.0
100	30.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.43939292419044E-4
2	3.2141867279307144E-4
3	0.0013186407088946518
4	0.0015741273462429907
5	0.008348643988189266
6	0.013021577000334688
7	0.01345013523072545
8	0.00911510390023428
9	0.0024065192937327397
10-11	0.005896796420088272
12-13	0.010479072883497188
14-15	0.03362945882902892
16-17	0.014509168550056467
18-19	0.022111956387277195
20-21	0.01710112169347752
22-23	0.030530653163126486
24-25	0.026133810549405882
26-27	0.022161405413860743
28-29	0.02935623878176719
30-31	0.03266108205843441
32-33	0.03729280754842688
34-35	0.01826317381819093
36-37	0.028482639312124483
38-39	0.02819418665705378
40-41	0.03171742980113167
42-43	0.03977350038203494
44-45	0.027864526479830114
46-47	0.027959303780781916
48-49	0.021905918776512406
50-51	0.035339570998376674
52-53	0.028243635683637327
54-55	0.03073257002167598
56-57	0.023508891388262464
58-59	0.025161313026596077
60-61	0.03314321006762402
62-63	0.0295375518792402
64-65	0.028816420241563438
66-67	0.024262989043661594
68-69	0.026372814177893035
70-71	0.02939332555170485
72-73	0.03623789498131115
74-75	0.03132595834067858
76-77	0.033485232501493566
78-79	0.03471321666165171
80-81	0.04048226976306581
82-83	0.04218414042798297
84-85	0.04717437136070617
86-87	0.04836114799871136
88-89	0.050557508929464014
90-91	0.05031438454876156
92-93	0.05089541061111827
94-95	0.05171544030196213
96-97	0.05237063990419416
98-99	0.05366455609979704
100-101	0.05738559535020914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.2133707E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.483945291778625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	37.15720896434343	7.239690266564537
2	5.236942100345413	2.040725867586845
3	6.708061304896446	3.920984984354984
4	7.732801859894338	6.02661953561381
5	7.658647873537358	7.461033808849929
6	6.6827517889940475	7.812382215317744
7	5.940159376998069	8.101641822811331
8	4.6634258220836635	7.268954687179667
9	4.096851177423624	7.184054178853266
>10	13.830597431602861	38.024297899267566
>50	0.22918552537682657	3.055352530946922
>100	0.06258046310167763	1.6843149398099957
>500	1.8526929271154086E-4	0.033219276351743904
>1k	6.010421096522723E-4	0.14672798649167437
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.4724513291774725E-5	0.0	0.0
2	2.4724513291774725E-5	0.0	0.0
3	2.4724513291774725E-5	8.241504430591574E-6	0.0
4	2.4724513291774725E-5	8.241504430591574E-6	0.0
5	2.4724513291774725E-5	8.241504430591574E-6	0.0
6	3.2966017722366295E-5	8.241504430591574E-6	0.0
7	3.2966017722366295E-5	8.241504430591574E-6	0.0
8	3.2966017722366295E-5	8.241504430591574E-6	0.0
9	4.944902658354945E-5	8.241504430591574E-6	0.0
10-11	5.769053101414102E-5	1.6483008861183147E-5	0.0
12-13	8.241504430591574E-5	1.6483008861183147E-5	9.88980531670989E-5
14-15	1.1538106202828205E-4	1.6483008861183147E-5	9.88980531670989E-5
16-17	1.9367535411890199E-4	1.6483008861183147E-5	9.88980531670989E-5
18-19	3.0493566393188825E-4	1.6483008861183147E-5	9.88980531670989E-5
20-21	4.285582303907619E-4	2.4724513291774725E-5	1.895546019036062E-4
22-23	6.057505756484807E-4	1.0713955759769047E-4	1.895546019036062E-4
24-25	8.241504430591575E-4	1.0713955759769047E-4	2.307621240565641E-4
26-27	0.00104667106268513	1.0713955759769047E-4	2.307621240565641E-4
28-29	0.0013268822133252435	1.0713955759769047E-4	2.307621240565641E-4
30-31	0.001668904647194794	1.0713955759769047E-4	2.307621240565641E-4
32-33	0.0019655988066960904	1.0713955759769047E-4	2.3488287627185987E-4
34-35	0.002303500488350345	1.0713955759769047E-4	2.3900362848715566E-4
36-37	0.0026084361522822333	1.1126030981298626E-4	2.3900362848715566E-4
38-39	0.0029628208427976708	1.6895084082712728E-4	3.214186727930714E-4
40-41	0.003461431860848461	1.8131309747301464E-4	3.2966017722366295E-4
42-43	0.003910593852315702	1.8131309747301464E-4	3.2966017722366295E-4
44-45	0.004326789826060577	1.8131309747301464E-4	3.791092038072124E-4
46-47	0.004742985799805451	1.8131309747301464E-4	3.8735070823780397E-4
48-49	0.005159181773550325	1.8131309747301464E-4	3.8735070823780397E-4
50-51	0.005707241818184666	1.8131309747301464E-4	4.0795446931428294E-4
52-53	0.006205852836235455	1.8131309747301464E-4	4.2855823039076186E-4
54-55	0.006712705358716837	1.8543384968831042E-4	4.2855823039076186E-4
56-57	0.00740499173088653	1.895546019036062E-4	4.944902658354944E-4
58-59	0.008002500802104419	1.895546019036062E-4	4.944902658354944E-4
60-61	0.008661821156551744	1.895546019036062E-4	4.944902658354944E-4
62-63	0.009374711289797916	1.895546019036062E-4	5.274562835578607E-4
64-65	0.010190620228426482	1.895546019036062E-4	5.645430534955229E-4
66-67	0.010816974565151441	1.895546019036062E-4	5.727845579261144E-4
68-69	0.011430966645230512	1.9367535411890199E-4	5.810260623567061E-4
70-71	0.012073803990816655	1.977961063341978E-4	5.851468145720018E-4
72-73	0.012922678947167587	1.977961063341978E-4	5.851468145720018E-4
74-75	0.013874572708900915	1.977961063341978E-4	5.851468145720018E-4
76-77	0.01473168916968244	1.977961063341978E-4	5.851468145720018E-4
78-79	0.01583605076338171	2.1015836298008515E-4	6.057505756484807E-4
80-81	0.016614872932072613	2.1427911519538093E-4	6.263543367249596E-4
82-83	0.017554404437160054	2.1427911519538093E-4	6.345958411555512E-4
84-85	0.018543384968831043	2.1427911519538093E-4	6.345958411555512E-4
86-87	0.01970131634132916	2.1427911519538093E-4	6.345958411555512E-4
88-89	0.020846885457181386	2.1427911519538093E-4	6.345958411555512E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	955	0.0	14.921927	32-33
CGATCTA	540	0.0	13.634399	34-35
CCGATCT	920	0.0	13.424327	32-33
TGACCGG	605	1.1370503E-8	11.775645	8
CTTCCGA	1395	0.0	10.044803	30-31
GACCGGA	620	2.509989E-5	9.193131	9
TTCCGAT	1320	0.0	9.176136	30-31
GTCAGCG	845	1.549979E-7	8.9913025	3
GACGTGT	1390	0.0	8.543394	20-21
GGTCAGT	3925	0.0	8.347737	2
TACGATC	620	1.5057594E-8	7.6615267	32-33
TCCGACG	280	0.00805609	7.6342583	18-19
GTGACCG	875	2.1471717E-5	7.597776	7
TCCGAAC	660	5.988113E-9	7.557051	32-33
TCAGACG	2085	0.0	7.2904835	18-19
CAGACGT	1900	0.0	6.3752756	18-19
CAGTGAC	4685	0.0	6.2840757	5
CGATCTG	850	1.130702E-7	6.147122	34-35
TGGTCAG	7530	0.0	5.8646717	1
GTGACTG	3535	0.0	5.6419125	7
>>END_MODULE