FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 2-58885.34200000001aca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 2-58885.34200000001aca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12133707
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC9550.014.92192732-33
CGATCTA5400.013.63439934-35
CCGATCT9200.013.42432732-33
TGACCGG6051.1370503E-811.7756458
CTTCCGA13950.010.04480330-31
GACCGGA6202.509989E-59.1931319
TTCCGAT13200.09.17613630-31
GTCAGCG8451.549979E-78.99130253
GACGTGT13900.08.54339420-21
GGTCAGT39250.08.3477372
TACGATC6201.5057594E-87.661526732-33
TCCGACG2800.008056097.634258318-19
GTGACCG8752.1471717E-57.5977767
TCCGAAC6605.988113E-97.55705132-33
TCAGACG20850.07.290483518-19
CAGACGT19000.06.375275618-19
CAGTGAC46850.06.28407575
CGATCTG8501.130702E-76.14712234-35
TGGTCAG75300.05.86467171
GTGACTG35350.05.64191257
Produced by FastQC (version 0.11.2)