##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l02n02 12-60303.34200000001c0e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10380212
Sequences flagged as poor quality	0
Sequence length	101
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.33825995076016	38.0	32.0	38.0	32.0	38.0
2	35.77619907955637	38.0	38.0	38.0	32.0	38.0
3	35.642534661141795	38.0	32.0	38.0	32.0	38.0
4	35.524312412887134	38.0	32.0	38.0	32.0	38.0
5	35.69074090201626	38.0	32.0	38.0	32.0	38.0
6	37.77447483731546	40.0	38.0	40.0	38.0	40.0
7	37.90273609055384	40.0	38.0	40.0	38.0	40.0
8	38.01987396789198	40.0	38.0	40.0	38.0	40.0
9	38.029010197479586	40.0	38.0	40.0	38.0	40.0
10-11	38.059237855643026	40.0	38.0	40.0	38.0	40.0
12-13	38.01399590875408	40.0	38.0	40.0	38.0	40.0
14-15	37.980654152342936	40.0	38.0	40.0	38.0	40.0
16-17	37.961047182851374	40.0	38.0	40.0	38.0	40.0
18-19	37.93783498834128	40.0	38.0	40.0	38.0	40.0
20-21	37.92888420775992	40.0	38.0	40.0	38.0	40.0
22-23	37.912811270135904	40.0	38.0	40.0	35.0	40.0
24-25	37.84978355933386	40.0	38.0	40.0	32.0	40.0
26-27	37.831627668105426	40.0	38.0	40.0	32.0	40.0
28-29	37.76251048629835	40.0	38.0	40.0	32.0	40.0
30-31	37.72670105388984	40.0	38.0	40.0	32.0	40.0
32-33	37.728737765664135	40.0	38.0	40.0	32.0	40.0
34-35	37.65205315652513	40.0	38.0	40.0	32.0	40.0
36-37	37.662934822525784	40.0	38.0	40.0	32.0	40.0
38-39	37.61455026159389	40.0	38.0	40.0	32.0	40.0
40-41	37.55286934409432	40.0	38.0	40.0	32.0	40.0
42-43	37.38908887410007	40.0	38.0	40.0	32.0	40.0
44-45	37.31063874225305	40.0	38.0	40.0	32.0	40.0
46-47	37.23426067791294	40.0	38.0	40.0	32.0	40.0
48-49	37.15756118468486	40.0	38.0	40.0	32.0	40.0
50-51	37.10300242422794	38.0	38.0	40.0	32.0	40.0
52-53	37.02874893113936	38.0	38.0	40.0	32.0	40.0
54-55	36.97851941752249	38.0	38.0	40.0	32.0	40.0
56-57	36.95509388440236	38.0	38.0	40.0	32.0	40.0
58-59	36.89034380993375	38.0	38.0	40.0	32.0	40.0
60-61	36.85586392647858	38.0	38.0	40.0	32.0	40.0
62-63	36.78513386817148	38.0	38.0	40.0	32.0	40.0
64-65	36.770450786554264	38.0	38.0	40.0	32.0	40.0
66-67	36.699358259734964	38.0	38.0	40.0	32.0	40.0
68-69	36.64774635624012	38.0	38.0	40.0	32.0	40.0
70-71	36.54084430067517	38.0	38.0	40.0	32.0	40.0
72-73	36.47444989562834	38.0	38.0	40.0	32.0	40.0
74-75	36.390261056325244	38.0	38.0	40.0	29.5	40.0
76-77	36.31010985132096	38.0	38.0	40.0	27.0	40.0
78-79	36.22551336138414	38.0	38.0	40.0	27.0	40.0
80-81	36.152575496531284	38.0	38.0	40.0	27.0	40.0
82-83	36.065276460634905	38.0	38.0	40.0	27.0	40.0
84-85	35.965746364332446	38.0	38.0	40.0	27.0	40.0
86-87	35.90735208490925	38.0	38.0	40.0	27.0	40.0
88-89	35.83871552912407	38.0	38.0	40.0	27.0	40.0
90-91	35.72371869668943	38.0	38.0	40.0	27.0	40.0
92-93	35.62114155279295	38.0	38.0	40.0	27.0	40.0
94-95	35.52369079745192	38.0	38.0	40.0	27.0	40.0
96-97	35.449017418912064	38.0	38.0	40.0	27.0	40.0
98-99	35.31584841427131	38.0	38.0	40.0	27.0	40.0
100-101	33.494938301838154	38.0	32.5	39.0	20.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.09070793692573176
2201	2	0.2317155442772929
2201	3	0.3403216454469984
2201	4	0.2629447926715045
2201	5	0.30213529105473214
2201	6	0.3217618529886792
2201	7	0.33795232141148546
2201	8	0.23047501491704736
2201	9	0.29160232212734627
2201	10-11	0.19453815509669425
2201	12-13	0.27975813588330567
2201	14-15	0.2545964419049369
2201	16-17	0.19953831659143617
2201	18-19	0.19627991587498173
2201	20-21	0.25839956153396315
2201	22-23	0.25009053382187574
2201	24-25	0.22378980170947926
2201	26-27	0.20650122993652076
2201	28-29	0.22505261402947951
2201	30-31	0.20796734646551585
2201	32-33	0.201449561244317
2201	34-35	0.22208742283665828
2201	36-37	0.15062327566914746
2201	38-39	0.19185990487995497
2201	40-41	0.17445373319318946
2201	42-43	0.2450167348941008
2201	44-45	0.19480589398926895
2201	46-47	0.20565398594126805
2201	48-49	0.1305209583614797
2201	50-51	0.1908187920199964
2201	52-53	0.18527306221140094
2201	54-55	0.26128447917333375
2201	56-57	0.1118981321471324
2201	58-59	0.1629328795781646
2201	60-61	0.082662551625873
2201	62-63	0.1911511756485993
2201	64-65	0.13750884740470326
2201	66-67	0.10294219723658671
2201	68-69	0.053894254845907597
2201	70-71	0.115511360499859
2201	72-73	-0.016509085618352515
2201	74-75	0.04855793674261122
2201	76-77	-0.005630722635473262
2201	78-79	0.04673802250322012
2201	80-81	0.19809351165099542
2201	82-83	0.06062669675504395
2201	84-85	-0.019088896581514803
2201	86-87	0.012821882345889435
2201	88-89	0.024485200663050932
2201	90-91	-0.01309485771388097
2201	92-93	-0.12544484690389623
2201	94-95	-0.040278651770549345
2201	96-97	-0.22609940488238323
2201	98-99	-0.13758922584638356
2201	100-101	-0.07728435039744852
1101	1	0.0342836416239507
1101	2	0.12596553964174717
1101	3	0.1354134653181589
1101	4	0.03656854974554591
1101	5	0.20512636092160363
1101	6	0.18274803180179333
1101	7	0.22544270121686338
1101	8	0.21021811298941628
1101	9	0.245974051518985
1101	10-11	0.21465834198166078
1101	12-13	0.21396757735052319
1101	14-15	0.2029598184224497
1101	16-17	0.18860695929986093
1101	18-19	0.19133218189181633
1101	20-21	0.1844426642304171
1101	22-23	0.22167439465368233
1101	24-25	0.19036590804892484
1101	26-27	0.16035509561948658
1101	28-29	0.20920652955119579
1101	30-31	0.15420326793720562
1101	32-33	0.1212139148865532
1101	34-35	0.12064803706964966
1101	36-37	0.16288393429879022
1101	38-39	0.17383154333612794
1101	40-41	0.15771283598242292
1101	42-43	0.18750420668389012
1101	44-45	0.1451944420960416
1101	46-47	0.14154309996352765
1101	48-49	0.10501779912956977
1101	50-51	0.08057758639209567
1101	52-53	0.14775882664486772
1101	54-55	0.07591818324191735
1101	56-57	0.13823905274437465
1101	58-59	0.01804960507120512
1101	60-61	-0.008415223326565524
1101	62-63	0.04647544217859689
1101	64-65	0.12327956041119137
1101	66-67	0.07144037469647913
1101	68-69	0.031996259282827566
1101	70-71	0.0730853544342267
1101	72-73	0.07282957415655744
1101	74-75	0.14076575171779382
1101	76-77	0.203715081911497
1101	78-79	0.09491415575624274
1101	80-81	0.1466421133131135
1101	82-83	0.10799824731067531
1101	84-85	0.10222183357006998
1101	86-87	0.1380397991892437
1101	88-89	0.08115619380809136
1101	90-91	0.07458191197673614
1101	92-93	0.07077048984468348
1101	94-95	0.057141589227533984
1101	96-97	0.06970069734976647
1101	98-99	0.04840567631605097
1101	100-101	0.14019564049944933
2202	1	-0.05436247261420135
2202	2	0.06194905192588607
2202	3	0.17140042060288607
2202	4	0.025256548715788085
2202	5	0.1351247735022767
2202	6	0.12653821829464107
2202	7	0.17354057426615555
2202	8	0.11573309399966547
2202	9	0.15939641341027766
2202	10-11	0.13880957191688026
2202	12-13	0.13591081106211078
2202	14-15	0.15832322299841906
2202	16-17	0.13574258009492723
2202	18-19	0.1311732583949663
2202	20-21	0.1265573008929337
2202	22-23	0.15493723347854882
2202	24-25	0.1335847520206883
2202	26-27	0.11761359057150145
2202	28-29	0.14864713405238206
2202	30-31	0.11502587662211994
2202	32-33	0.13431240849814685
2202	34-35	0.14117362344118334
2202	36-37	0.10791274961570707
2202	38-39	0.06448382254486518
2202	40-41	0.05827114161124314
2202	42-43	0.048116145280857836
2202	44-45	-0.005173979156062103
2202	46-47	-0.04166699313356759
2202	48-49	-0.02662130952919739
2202	50-51	0.06464261394560822
2202	52-53	0.02717412399715613
2202	54-55	0.06102623758496861
2202	56-57	0.0188477022711524
2202	58-59	-0.01781311369475702
2202	60-61	-0.0550214393617523
2202	62-63	-0.028202036311007816
2202	64-65	-0.09418196658715772
2202	66-67	-0.03716180535582936
2202	68-69	-0.07465240349579716
2202	70-71	-0.04217110413158309
2202	72-73	-0.11054229910134694
2202	74-75	-0.09775878499831236
2202	76-77	-0.11562553769715578
2202	78-79	-0.05732531032948174
2202	80-81	-0.022623372550135912
2202	82-83	-0.1432752846459593
2202	84-85	-0.11521184097903614
2202	86-87	-0.00689360458297017
2202	88-89	-0.024301902040946288
2202	90-91	-0.05771649942707313
2202	92-93	-0.07443629848754796
2202	94-95	-0.08755174832503343
2202	96-97	-0.1522766773832629
2202	98-99	-0.12632240703314324
2202	100-101	-0.027515218948671816
1102	1	-0.17503897345306996
1102	2	0.0046348897467396455
1102	3	-0.05838125430684471
1102	4	-0.1821047709089143
1102	5	-0.029441580420936475
1102	6	-0.11034474000457806
1102	7	0.08629542401585155
1102	8	0.1165000875293245
1102	9	0.14390629306387126
1102	10-11	0.06600447758205519
1102	12-13	0.153939297550032
1102	14-15	0.13635566215430117
1102	16-17	0.12911036587429692
1102	18-19	0.14775057200328234
1102	20-21	0.1949340239213413
1102	22-23	0.1671832460051803
1102	24-25	0.17566652040699893
1102	26-27	0.1316750278044836
1102	28-29	0.014295807491265577
1102	30-31	0.0933602317350406
1102	32-33	0.08264159978152463
1102	34-35	0.10985509677313843
1102	36-37	0.09943276117394362
1102	38-39	0.08600072807752923
1102	40-41	0.09397039095941295
1102	42-43	0.0026661706606105895
1102	44-45	-0.03404142724445336
1102	46-47	-0.05143932891253655
1102	48-49	-0.10989028068161133
1102	50-51	-0.03245578320904485
1102	52-53	-0.0127408893541201
1102	54-55	-0.00994973968799684
1102	56-57	-0.0792447541115493
1102	58-59	-0.0757625443184935
1102	60-61	-0.07724139820730613
1102	62-63	-0.12486167344826526
1102	64-65	-0.12614339077963166
1102	66-67	-0.15348115729905487
1102	68-69	-0.3400333692224393
1102	70-71	-0.08706256843808546
1102	72-73	-0.11177397513916532
1102	74-75	-0.028739342706252557
1102	76-77	-0.11938514198480021
1102	78-79	-0.12218071074457271
1102	80-81	-0.05356424169856666
1102	82-83	-0.1706526411891076
1102	84-85	-0.1297567238723687
1102	86-87	-0.09468594558710208
1102	88-89	-0.3103669846780974
1102	90-91	-0.35370707276545943
1102	92-93	-0.19621761631682233
1102	94-95	-0.1880502218514195
1102	96-97	-0.23447847106930908
1102	98-99	-0.2946857084697285
1102	100-101	-0.33777444563902037
2203	1	-0.24778907406145123
2203	2	0.03458528530634908
2203	3	-0.08462603102991295
2203	4	-0.23667079140189173
2203	5	-0.08664222332206606
2203	6	-0.14534736327107822
2203	7	0.0017480632390842743
2203	8	0.02764424194401016
2203	9	0.08628982627239168
2203	10-11	0.08007478935724066
2203	12-13	0.08866063422446047
2203	14-15	0.03254979049222584
2203	16-17	0.08740511772798243
2203	18-19	0.027844478831177355
2203	20-21	0.07255637420328043
2203	22-23	0.037887755841751414
2203	24-25	0.037079444249066285
2203	26-27	0.053427998609713256
2203	28-29	-0.016297729715923026
2203	30-31	-0.04378877511661727
2203	32-33	-0.022724280512363748
2203	34-35	-0.05225246292410901
2203	36-37	-0.08010110841608764
2203	38-39	-0.06166868404373105
2203	40-41	-0.006840107457044553
2203	42-43	-0.043830014786564675
2203	44-45	-0.16392603413033413
2203	46-47	-0.17160207315874487
2203	48-49	-0.22030590264693473
2203	50-51	-0.1779457598326104
2203	52-53	-0.1655644257383031
2203	54-55	-0.20671681410978948
2203	56-57	-0.3148901881880235
2203	58-59	-0.15433490666411842
2203	60-61	-0.2383505770028762
2203	62-63	-0.24873207099214767
2203	64-65	-0.37065934394478006
2203	66-67	-0.29850937066409244
2203	68-69	-0.16352487642625846
2203	70-71	-0.20987829515344458
2203	72-73	-0.25848496355558837
2203	74-75	-0.2852554745237086
2203	76-77	-0.27234313105648766
2203	78-79	-0.30096229079815373
2203	80-81	-0.2614378505917969
2203	82-83	-0.3039020471383438
2203	84-85	-0.3374423352477649
2203	86-87	-0.4536585618258755
2203	88-89	-0.33067468332903616
2203	90-91	-0.31082305403849375
2203	92-93	-0.42567490650834117
2203	94-95	-0.3226323305587826
2203	96-97	-0.3855006085827384
2203	98-99	-0.42366601639758983
2203	100-101	-0.28549731750450036
1103	1	-0.2589835337808424
1103	2	0.0027243803466276972
1103	3	-0.059165413920155174
1103	4	-0.15587327340296042
1103	5	-0.02745176546122252
1103	6	-0.06408339629143711
1103	7	0.018621593079181764
1103	8	0.040586528240460495
1103	9	0.06978415537233928
1103	10-11	0.09058612399424248
1103	12-13	0.09850913163214159
1103	14-15	0.12348973146251296
1103	16-17	0.08409430716890398
1103	18-19	0.059579594524763024
1103	20-21	0.1329318143238183
1103	22-23	0.10465784257617372
1103	24-25	0.07790639709646996
1103	26-27	0.019372952613522898
1103	28-29	0.039970041493631925
1103	30-31	0.11503313220924127
1103	32-33	0.11222450159534958
1103	34-35	0.10516126600726494
1103	36-37	0.07841450839740105
1103	38-39	0.1162680356686252
1103	40-41	0.018297920201860052
1103	42-43	-0.08484173013032859
1103	44-45	-0.1043822243458763
1103	46-47	-0.1408763702692042
1103	48-49	-0.04632078756439739
1103	50-51	-0.09855311329665994
1103	52-53	-0.08521798921490387
1103	54-55	-0.035114882118485014
1103	56-57	-0.05150164464551921
1103	58-59	-0.09447123536122604
1103	60-61	-0.22434924646756826
1103	62-63	-0.16734028252825084
1103	64-65	-0.14882394625164608
1103	66-67	-0.15659748156293318
1103	68-69	-0.22629838655108614
1103	70-71	-0.18323578759329706
1103	72-73	-0.15692650903988437
1103	74-75	-0.23726049020277884
1103	76-77	-0.3266249720443426
1103	78-79	-0.337538555096657
1103	80-81	-0.20330585339365825
1103	82-83	-0.3135227072644824
1103	84-85	-0.25792985192236273
1103	86-87	-0.36877458047112555
1103	88-89	-0.31693043753858774
1103	90-91	-0.3130179525796848
1103	92-93	-0.28862390461703313
1103	94-95	-0.16861819585935933
1103	96-97	-0.36407060315881523
1103	98-99	-0.36904260485867724
1103	100-101	-0.28319228326908785
2204	1	-0.06313374901652224
2204	2	-0.005698426403185408
2204	3	-0.0035767818547967067
2204	4	-0.15925281037086592
2204	5	0.03349427309725428
2204	6	-0.03569081214772751
2204	7	0.03697395301892925
2204	8	0.06069535958458516
2204	9	0.05154578886390482
2204	10-11	0.0816708249754754
2204	12-13	0.13860360383396397
2204	14-15	0.10437737288487625
2204	16-17	0.044121918700213314
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1213	24-25	-0.3740896577865982
1213	26-27	-0.26132137717538484
1213	28-29	-0.2551227677362249
1213	30-31	-0.4833725346144675
1213	32-33	-0.37697056207078106
1213	34-35	-0.4655231162953797
1213	36-37	-0.48063973772707413
1213	38-39	-0.6499890080807162
1213	40-41	-0.43825859892572794
1213	42-43	-0.17589244354159916
1213	44-45	-0.26787251311481697
1213	46-47	-0.2974096604054992
1213	48-49	-0.18016154034919651
1213	50-51	-0.373415521194147
1213	52-53	-0.3844783074549767
1213	54-55	-0.14113377001437755
1213	56-57	-0.2244113175914535
1213	58-59	-0.29491098236331936
1213	60-61	-0.17403884557262472
1213	62-63	-0.38302671757375606
1213	64-65	-0.35280305683789237
1213	66-67	-0.21757815833952066
1213	68-69	-0.15124189539383082
1213	70-71	-0.11855499331165475
1213	72-73	-0.12004711496150122
1213	74-75	-0.17199412709452844
1213	76-77	-0.1493214616585945
1213	78-79	-0.10755548849392227
1213	80-81	-0.06533249981749378
1213	82-83	0.01642456440072948
1213	84-85	0.09919859061773906
1213	86-87	0.017560333450276744
1213	88-89	-0.038556757535673114
1213	90-91	-0.02361465865779877
1213	92-93	-0.11305860872134588
1213	94-95	-0.09544409595827119
1213	96-97	-0.1324354046560572
1213	98-99	-0.0367083241599957
1213	100-101	-0.6482397762208834
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41.0
3	48.0
4	85.0
5	84.0
6	93.0
7	65.0
8	46.0
9	65.0
10	55.0
11	62.0
12	70.0
13	122.0
14	614.0
15	4692.0
16	17170.0
17	28715.0
18	33213.0
19	34748.0
20	37392.0
21	42529.0
22	48480.0
23	55790.0
24	63945.0
25	73406.0
26	83384.0
27	95364.0
28	108006.0
29	125152.0
30	145156.0
31	168810.0
32	199709.0
33	241430.0
34	301660.0
35	401022.0
36	581769.0
37	1005970.0
38	2514495.0
39	3966755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.222014432541174	35.596360131073205	35.34166256107568	14.839962875309942
2	15.005713781455905	37.168191722560124	33.751512259052504	14.074582236931471
3	15.183669710222656	35.38243902523614	34.23879581579051	15.195095448750697
4	15.839732162468989	34.49072950120347	34.47759851036953	15.191939825958013
5	15.704167375207323	34.699399719538334	34.63311403498208	14.963318870272268
6	15.680406041835921	35.04181395363178	33.546202501399	15.731577503133302
7	15.879352692636918	34.727618398673904	34.415316760749946	14.977712147939231
8	15.393525220404857	35.29210116969572	34.264613007078104	15.049760602821316
9	15.408685425113228	34.71793351113742	34.53897335289409	15.334407710855258
10-11	15.363165320005992	35.095835195767144	34.31505573428015	15.22594374994671
12-13	15.251119295744292	34.99085322761546	34.55779905542606	15.20022842121419
14-15	15.32385735505728	34.90522648449629	34.46773019775777	15.303185962688667
16-17	15.387312479892687	34.75999420735975	34.61534199846281	15.237351314284744
18-19	15.398380168689668	34.60917673149252	34.492188111649405	15.500254988168404
20-21	15.55857667000227	34.5332700161532	34.30364968750351	15.604503626341021
22-23	15.508116108115066	34.603886203146224	34.28158262807292	15.60641506066579
24-25	15.760496080268787	34.41615887010984	34.162391886201924	15.660953163419453
26-27	15.752684017231209	34.496446010248306	34.11071820406298	15.640151768457514
28-29	15.7859692469054	34.42117256707205	34.058719564324015	15.734138621698543
30-31	15.742427324027314	34.39566156544621	34.06155665740131	15.800354453125163
32-33	15.843893971327851	34.44592736346021	33.945691561828866	15.764487103383077
34-35	15.872045131295218	34.46996811505751	33.92015014473053	15.73783660891674
36-37	15.851589039522368	34.394170611528416	33.92515735440309	15.829082994546125
38-39	15.934571585949357	34.401487144321344	33.903515821795544	15.760425447933759
40-41	15.908269150449971	34.502028456012404	33.83991088632031	15.749791507217308
42-43	15.869126789753135	34.31250850519449	33.96187282744083	15.856491877611553
44-45	15.959243130205387	34.41996701741162	33.82511056471517	15.79567928766783
46-47	15.979960352612455	34.476271771407944	33.816302686213376	15.727465189766226
48-49	15.898923750188862	34.30278395758366	33.84778771942808	15.950504572799398
50-51	15.987595554795927	34.33534029946586	33.78358563482481	15.8934785109134
52-53	15.979249612517844	34.45980207476892	33.782035937837875	15.778912374875354
54-55	15.98744821787581	34.27909525983514	33.8333407616113	15.90011576067775
56-57	16.041677607536506	34.384556197366386	33.72612862801251	15.847637567084597
58-59	16.03135456085393	34.38014116712016	33.78435960661149	15.804144665414421
60-61	16.02458007086109	34.33665191241156	33.709501709128375	15.929266307598972
62-63	16.007750477562904	34.318200718943814	33.730121139999	15.943927663494284
64-65	15.98276729600355	34.401561570457204	33.739863820317254	15.87580731322199
66-67	16.079935461751628	34.33733819425881	33.689011904539356	15.893714439450198
68-69	16.008919495296215	34.33392342447256	33.730902058907674	15.926255021323552
70-71	16.08705971643923	34.36878202701775	33.64524703059869	15.898911225944323
72-73	16.066953376949108	34.290464330050625	33.70277265974872	15.939809633251546
74-75	16.075364650644737	34.36375125072432	33.630441090462966	15.930443008167977
76-77	16.059639784636147	34.4092226435321	33.67066873301403	15.860468838817726
78-79	16.10122437367921	34.2617047964068	33.69281966468534	15.944251165228648
80-81	16.160353731330552	34.33511367398867	33.632386937156625	15.87214565752415
82-83	16.09009559144411	34.460154682654604	33.63326131977566	15.816488406125625
84-85	16.03969064769801	34.28104976916068	33.698434757079816	15.980824826061497
86-87	16.076151816122135	34.469928419000766	33.58275737085548	15.87116239402161
88-89	16.105047734183525	34.433923595908496	33.61875448950003	15.842274180407962
90-91	16.06847821283371	34.26717402335308	33.65558550429571	16.008762259517503
92-93	16.11694650028458	34.37520692940617	33.62889238128698	15.87895418902227
94-95	16.09833557610295	34.42188544746941	33.587562619152656	15.892216357274982
96-97	16.08144939391955	34.339527562940994	33.66456102282694	15.914462020312516
98-99	16.10368486140684	34.41071519796889	33.55503002040558	15.930569920218687
100-101	16.18399078870064	34.480666729964625	33.463789790360046	15.871552690974692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1172.0
1	1307.5
2	1648.5
3	2027.0
4	2439.0
5	2989.0
6	3528.5
7	4262.0
8	5464.0
9	7125.5
10	9609.5
11	13553.0
12	19672.0
13	29045.5
14	43136.5
15	63271.5
16	90278.5
17	124401.5
18	164258.0
19	208440.0
20	256121.5
21	301602.5
22	344245.5
23	381732.5
24	409148.0
25	427939.5
26	437399.0
27	438039.0
28	438289.5
29	439696.5
30	436295.0
31	431948.0
32	423428.5
33	402618.5
34	372202.0
35	336730.0
36	305274.5
37	281770.0
38	260028.5
39	240717.0
40	225011.5
41	209438.5
42	192836.0
43	175735.5
44	157834.5
45	141406.5
46	128426.5
47	121525.5
48	113652.5
49	101151.5
50	88357.0
51	76134.0
52	65880.5
53	57800.5
54	51585.0
55	46048.5
56	41522.5
57	37467.0
58	33512.5
59	29258.0
60	24854.0
61	20332.5
62	16250.5
63	13001.0
64	10346.5
65	8440.0
66	6864.0
67	5294.5
68	3966.0
69	3030.0
70	2371.0
71	1809.5
72	1361.0
73	1113.5
74	927.0
75	760.5
76	598.0
77	436.0
78	362.0
79	285.5
80	228.0
81	195.0
82	128.0
83	94.0
84	73.0
85	46.0
86	31.0
87	21.0
88	18.0
89	12.5
90	8.5
91	8.5
92	7.5
93	7.5
94	6.5
95	4.0
96	3.5
97	3.5
98	6.0
99	9.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.587554473839262E-4
2	4.4315087206311395E-4
3	0.0013390863307994095
4	0.0018111383466927265
5	0.008641442005230721
6	0.013294526161893418
7	0.014103758189139105
8	0.009797487758438845
9	0.002389161223296788
10-11	0.0062474639246288995
12-13	0.01099206837008724
14-15	0.034223771152265485
16-17	0.014691414780353235
18-19	0.02196005245364931
20-21	0.01710466029017519
22-23	0.030770084464556216
24-25	0.02676727604407309
26-27	0.022138276173935562
28-29	0.03027394816213773
30-31	0.032576405953943906
32-33	0.03762447240961938
34-35	0.018872446921122613
36-37	0.028901143830203084
38-39	0.028978213547083624
40-41	0.032311478802167046
42-43	0.040461601362284313
44-45	0.028115996089482564
46-47	0.028742187539136963
48-49	0.02238875275379732
50-51	0.036323920937260244
52-53	0.028515795245800377
54-55	0.030823069894911587
56-57	0.023670036796936324
58-59	0.024883884837804855
60-61	0.033058091684447295
62-63	0.02933466098765613
64-65	0.028578414390765815
66-67	0.02398794937906856
68-69	0.02619407002477406
70-71	0.02924795755616552
72-73	0.036295019793430035
74-75	0.031068729617468312
76-77	0.033212231118208375
78-79	0.03468618945354873
80-81	0.04047605193419942
82-83	0.04227273970897704
84-85	0.04741232645344816
86-87	0.04857800592126635
88-89	0.05115020772215442
90-91	0.050697423135481236
92-93	0.051029786289528575
94-95	0.051935355462874935
96-97	0.052749404347425664
98-99	0.05396806924559922
100-101	0.057773386516575966
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.0380212E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.9638504534698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.180583098596045	21.663845332062472
2	23.752532450803653	21.36010634018248
3	13.683923556488345	18.458456772319636
4	6.890911513467592	12.393676591186003
5	3.0353944898877065	6.824151195529855
6	1.6545388070830442	4.46366612946866
7	0.9617114480013181	3.026957480912482
8	0.4626542541553557	1.664217335640242
9	0.293861997470165	1.1891850217385778
>10	1.0534171159362258	7.960536693915338
>50	0.028055590328482045	0.8059546735251508
>100	0.0023530397898528536	0.1739812999217213
>500	6.263799218162042E-5	0.015265133597327155
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.926742922013539E-5	0.0	9.633714610067695E-6
2	1.926742922013539E-5	0.0	9.633714610067695E-6
3	1.926742922013539E-5	0.0	2.890114383020308E-5
4	1.926742922013539E-5	0.0	4.816857305033847E-5
5	1.926742922013539E-5	0.0	5.780228766040616E-5
6	1.926742922013539E-5	0.0	5.780228766040616E-5
7	1.926742922013539E-5	0.0	8.670343149060925E-5
8	2.890114383020308E-5	0.0	9.633714610067694E-5
9	2.890114383020308E-5	0.0	9.633714610067694E-5
10-11	2.890114383020308E-5	0.0	1.0115400340571079E-4
12-13	2.890114383020308E-5	0.0	1.0597086071074464E-4
14-15	5.780228766040616E-5	0.0	1.0597086071074464E-4
16-17	9.633714610067694E-5	0.0	1.0597086071074464E-4
18-19	1.637731483711508E-4	0.0	1.734068629812185E-4
20-21	1.9749114950638774E-4	0.0	1.7822372028625233E-4
22-23	2.4084286525169235E-4	1.445057191510154E-5	1.830405775912862E-4
24-25	3.179125821322339E-4	5.780228766040616E-5	1.830405775912862E-4
26-27	3.9498229901277547E-4	5.780228766040616E-5	1.830405775912862E-4
28-29	4.7686887319835084E-4	5.780228766040616E-5	2.1675857872652314E-4
30-31	5.394880181637909E-4	5.780228766040616E-5	2.2157543603155698E-4
32-33	6.93627451924874E-4	5.780228766040616E-5	2.360260079466585E-4
34-35	7.610634541953478E-4	5.780228766040616E-5	2.6492715177686157E-4
36-37	7.995983126356187E-4	5.780228766040616E-5	2.793777236919631E-4
38-39	8.670343149060926E-4	5.780228766040616E-5	2.793777236919631E-4
40-41	9.922726048369726E-4	5.780228766040616E-5	2.793777236919631E-4
42-43	0.0011512288959030894	5.780228766040616E-5	2.9382829560706464E-4
44-45	0.0013487200454094773	5.780228766040616E-5	3.179125821322339E-4
46-47	0.0015365774803057972	5.780228766040616E-5	3.46813725962437E-4
48-49	0.0017099843432870157	5.780228766040616E-5	3.46813725962437E-4
50-51	0.0019267429220135388	5.780228766040616E-5	3.46813725962437E-4
52-53	0.0021964869310954344	7.706971688054156E-5	3.46813725962437E-4
54-55	0.0024132455098219573	7.706971688054156E-5	3.46813725962437E-4
56-57	0.0027022569481239883	7.706971688054156E-5	3.660811551825724E-4
58-59	0.0029960852437310526	7.706971688054156E-5	3.660811551825724E-4
60-61	0.003323631540473354	7.706971688054156E-5	3.7571486979264007E-4
62-63	0.0036463609799106222	7.706971688054156E-5	4.142497282329109E-4
64-65	0.003925738703602586	7.706971688054156E-5	4.142497282329109E-4
66-67	0.0042292007138197175	1.2523828993088E-4	4.142497282329109E-4
68-69	0.0046241830128324925	1.2523828993088E-4	4.287003001480124E-4
70-71	0.005076967599505675	1.2523828993088E-4	4.383340147580801E-4
72-73	0.005491217327738586	1.2523828993088E-4	4.4796772936814776E-4
74-75	0.005857298482921158	1.2523828993088E-4	4.527845866731816E-4
76-77	0.006223379638103731	1.2523828993088E-4	4.576014439782155E-4
78-79	0.0068447542304530965	1.3005514723591385E-4	5.298543035537231E-4
80-81	0.007297538817126278	1.3487200454094772E-4	5.298543035537231E-4
82-83	0.007706971688054155	1.3487200454094772E-4	5.298543035537231E-4
84-85	0.008241642848912913	1.3487200454094772E-4	5.298543035537231E-4
86-87	0.008805215153601872	1.3487200454094772E-4	5.34671160858757E-4
88-89	0.009551828035882119	1.3968886184598158E-4	5.394880181637909E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGCG	235	0.0	30.310308	3
GTAGCCG	740	0.0	27.592901	1
GCCGCGA	195	5.3842086E-10	26.787058	4
AGCCGTG	540	0.0	26.381193	3
GCCGTGA	430	0.0	25.399567	4
CCGTGAC	435	0.0	18.557804	5
TAGCCGC	440	0.0	18.346918	2
TAGCCGT	745	0.0	17.209743	2
CCGCGAC	190	0.0037639227	14.9956255	5
TAGCCGA	770	2.3646862E-11	11.7173605	2
GGAGCCG	500	1.9399977E-6	11.39651	1
CCGAGAC	505	2.1861506E-6	11.283837	5
ATAGCCG	610	1.612625E-7	10.898303	1
TCCGATC	610	1.2914825E-9	8.176761	32-33
CGTGACT	1020	2.6868365E-6	7.449238	6
CCGATCT	420	1.0611701E-4	7.3516674	32-33
AGCCGAA	1325	7.7478203E-4	5.37579	3
CCGAACT	595	0.0070448085	5.1894126	32-33
>>END_MODULE