FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 12-60303.34200000001c0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 12-60303.34200000001c0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10380212
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCG2350.030.3103083
GTAGCCG7400.027.5929011
GCCGCGA1955.3842086E-1026.7870584
AGCCGTG5400.026.3811933
GCCGTGA4300.025.3995674
CCGTGAC4350.018.5578045
TAGCCGC4400.018.3469182
TAGCCGT7450.017.2097432
CCGCGAC1900.003763922714.99562555
TAGCCGA7702.3646862E-1111.71736052
GGAGCCG5001.9399977E-611.396511
CCGAGAC5052.1861506E-611.2838375
ATAGCCG6101.612625E-710.8983031
TCCGATC6101.2914825E-98.17676132-33
CGTGACT10202.6868365E-67.4492386
CCGATCT4201.0611701E-47.351667432-33
AGCCGAA13257.7478203E-45.375793
CCGAACT5950.00704480855.189412632-33
Produced by FastQC (version 0.11.2)