##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n02 11-60308.34200000001be3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10132962 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.68501115468508 38.0 32.0 38.0 32.0 38.0 2 35.67472304741693 38.0 32.0 38.0 32.0 38.0 3 35.67317276034392 38.0 32.0 38.0 32.0 38.0 4 35.68043519752665 38.0 32.0 38.0 32.0 38.0 5 35.8327341008483 38.0 38.0 38.0 32.0 38.0 6 37.949525025357836 40.0 38.0 40.0 38.0 40.0 7 38.008419058514185 40.0 38.0 40.0 38.0 40.0 8 38.10964928122695 40.0 38.0 40.0 38.0 40.0 9 38.10547044388403 40.0 38.0 40.0 38.0 40.0 10-11 38.13657117237783 40.0 38.0 40.0 38.0 40.0 12-13 38.09424445685279 40.0 38.0 40.0 38.0 40.0 14-15 38.06922714207356 40.0 38.0 40.0 38.0 40.0 16-17 38.05703475449725 40.0 38.0 40.0 38.0 40.0 18-19 38.03581943759386 40.0 38.0 40.0 38.0 40.0 20-21 38.03070074673131 40.0 38.0 40.0 38.0 40.0 22-23 38.01645111271512 40.0 38.0 40.0 38.0 40.0 24-25 37.95454335070042 40.0 38.0 40.0 38.0 40.0 26-27 37.93737453076405 40.0 38.0 40.0 38.0 40.0 28-29 37.874803537208564 40.0 38.0 40.0 35.0 40.0 30-31 37.83796741762181 40.0 38.0 40.0 32.0 40.0 32-33 37.84152121561297 40.0 38.0 40.0 35.0 40.0 34-35 37.76580668120536 40.0 38.0 40.0 32.0 40.0 36-37 37.77770813706792 40.0 38.0 40.0 32.0 40.0 38-39 37.732146039825274 40.0 38.0 40.0 32.0 40.0 40-41 37.676413175140695 40.0 38.0 40.0 32.0 40.0 42-43 37.5253347441745 40.0 38.0 40.0 32.0 40.0 44-45 37.4506365956963 40.0 38.0 40.0 32.0 40.0 46-47 37.37618131796014 40.0 38.0 40.0 32.0 40.0 48-49 37.30003423480716 40.0 38.0 40.0 32.0 40.0 50-51 37.246947585513496 40.0 38.0 40.0 32.0 40.0 52-53 37.18053941187187 40.0 38.0 40.0 32.0 40.0 54-55 37.12713656678077 40.0 38.0 40.0 32.0 40.0 56-57 37.10598707465793 38.0 38.0 40.0 32.0 40.0 58-59 37.04188242292826 38.0 38.0 40.0 32.0 40.0 60-61 37.00615545582822 38.0 38.0 40.0 32.0 40.0 62-63 36.93598702926153 38.0 38.0 40.0 32.0 40.0 64-65 36.923605999904076 38.0 38.0 40.0 32.0 40.0 66-67 36.85740724183116 38.0 38.0 40.0 32.0 40.0 68-69 36.80552912366592 38.0 38.0 40.0 32.0 40.0 70-71 36.70305854300055 38.0 38.0 40.0 32.0 40.0 72-73 36.63909067259899 38.0 38.0 40.0 32.0 40.0 74-75 36.556127764023984 38.0 38.0 40.0 32.0 40.0 76-77 36.47913764010958 38.0 38.0 40.0 32.0 40.0 78-79 36.3956178854712 38.0 38.0 40.0 29.5 40.0 80-81 36.32632911284973 38.0 38.0 40.0 27.0 40.0 82-83 36.24477985805137 38.0 38.0 40.0 27.0 40.0 84-85 36.148181548494904 38.0 38.0 40.0 27.0 40.0 86-87 36.08942409929101 38.0 38.0 40.0 27.0 40.0 88-89 36.01978819223836 38.0 38.0 40.0 27.0 40.0 90-91 35.909305739032675 38.0 38.0 40.0 27.0 40.0 92-93 35.815055854349396 38.0 38.0 40.0 27.0 40.0 94-95 35.71962492309751 38.0 38.0 40.0 27.0 40.0 96-97 35.64796719853484 38.0 38.0 40.0 27.0 40.0 98-99 35.516749051264576 38.0 38.0 40.0 27.0 40.0 100-101 33.65643964716339 38.0 32.5 39.0 20.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.11384357201604445 2201 2 0.22033198113042118 2201 3 0.33880868278164655 2201 4 0.2806470209978613 2201 5 0.36228688898018646 2201 6 0.26403369008372124 2201 7 0.24367173370972495 2201 8 0.14214628793222062 2201 9 0.17971183121196788 2201 10-11 0.15848847957114742 2201 12-13 0.2069834984089738 2201 14-15 0.20472580593214929 2201 16-17 0.15309404094548995 2201 18-19 0.19018097759516195 2201 20-21 0.20794629567043899 2201 22-23 0.16533568653404984 2201 24-25 0.16839894726987126 2201 26-27 0.1909215505983468 2201 28-29 0.2337084452074052 2201 30-31 0.21022222782520572 2201 32-33 0.19200741689624579 2201 34-35 0.1974967708841291 2201 36-37 0.1318025456192231 2201 38-39 0.18293017195703243 2201 40-41 0.19735412334642888 2201 42-43 0.1863344186763456 2201 44-45 0.1806113838453598 2201 46-47 0.2216687064323466 2201 48-49 0.12412639254265656 2201 50-51 0.18406376822022708 2201 52-53 0.15299861037352258 2201 54-55 0.234606491621264 2201 56-57 0.09706395352912267 2201 58-59 0.16140942499639266 2201 60-61 0.07017968236001337 2201 62-63 0.14815502828778904 2201 64-65 0.11997290217970402 2201 66-67 0.13362567494500155 2201 68-69 0.07439493916459128 2201 70-71 0.22985440113375688 2201 72-73 0.07343379764988356 2201 74-75 0.06337226195644519 2201 76-77 0.11815895427552903 2201 78-79 0.1465918381137783 2201 80-81 0.20494967833270294 2201 82-83 0.13254990762379038 2201 84-85 0.10019917877554718 2201 86-87 0.14649382174560088 2201 88-89 0.15405432054389223 2201 90-91 0.12463067789071403 2201 92-93 0.06508694505627943 2201 94-95 0.127653040700892 2201 96-97 -0.10016568007957005 2201 98-99 0.004887153177705272 2201 100-101 0.12478149454545928 1101 1 0.2198867438983001 1101 2 0.14546721873086454 1101 3 0.20313946182270826 1101 4 0.19112005662008613 1101 5 0.31763194781897397 1101 6 0.35864349561594366 1101 7 0.32270798562811365 1101 8 0.3111344793169053 1101 9 0.23407995262878245 1101 10-11 0.29969685053659845 1101 12-13 0.2515528968775982 1101 14-15 0.29564484128601265 1101 16-17 0.27952638407155206 1101 18-19 0.29592140155990876 1101 20-21 0.2920413534923654 1101 22-23 0.299730798583802 1101 24-25 0.27314268027941324 1101 26-27 0.2559516016225132 1101 28-29 0.27805541663460076 1101 30-31 0.24596069814884203 1101 32-33 0.23824789977042826 1101 34-35 0.28199210400090635 1101 36-37 0.27564685977483805 1101 38-39 0.24392486160737548 1101 40-41 0.3001518431673489 1101 42-43 0.24880534135053267 1101 44-45 0.26431325578322173 1101 46-47 0.19965265433347668 1101 48-49 0.22770664582441213 1101 50-51 0.2714859853532232 1101 52-53 0.28103079433930134 1101 54-55 0.2132050499049356 1101 56-57 0.23945389869025746 1101 58-59 0.2240334302489373 1101 60-61 0.2175178214297233 1101 62-63 0.2781530815212605 1101 64-65 0.2399848886976912 1101 66-67 0.25767192576839903 1101 68-69 0.2219222036636168 1101 70-71 0.2741097859666226 1101 72-73 0.1943268761930952 1101 74-75 0.2904513776154971 1101 76-77 0.22917359052024722 1101 78-79 0.20402709357553306 1101 80-81 0.22965868107780096 1101 82-83 0.28341797461111895 1101 84-85 0.2551295383045087 1101 86-87 0.3167709854546885 1101 88-89 0.2489611337191704 1101 90-91 0.1620482930651761 1101 92-93 0.2272881011842216 1101 94-95 0.14726596013148452 1101 96-97 0.19605315646029453 1101 98-99 0.18242912400778266 1101 100-101 0.21708503883724006 2202 1 0.08028445247707339 2202 2 -0.0036932500561093207 2202 3 0.16815928652938794 2202 4 0.08349558581181782 2202 5 0.13387796606550495 2202 6 0.18905638553973603 2202 7 0.1998993863748808 2202 8 0.1603704710616256 2202 9 0.18723185050776436 2202 10-11 0.159707857742454 2202 12-13 0.13262040909377504 2202 14-15 0.18284538506647863 2202 16-17 0.19659814189240166 2202 18-19 0.1943864119745129 2202 20-21 0.16864668071404765 2202 22-23 0.22697988770533328 2202 24-25 0.23690187948371033 2202 26-27 0.17759976542298261 2202 28-29 0.17819631614329268 2202 30-31 0.16532701650054804 2202 32-33 0.19155980575938258 2202 34-35 0.18608954993555926 2202 36-37 0.19633093922253408 2202 38-39 0.14904643084451408 2202 40-41 0.16680586577865597 2202 42-43 0.11818620660397272 2202 44-45 0.11801574990500541 2202 46-47 0.054370734607871896 2202 48-49 0.12900759088272196 2202 50-51 0.15134958930961773 2202 52-53 0.1190140277004943 2202 54-55 0.1393034310716743 2202 56-57 0.143084294754658 2202 58-59 0.10244878942548752 2202 60-61 -0.013502764073393791 2202 62-63 0.1130690879035754 2202 64-65 0.07726532859859248 2202 66-67 0.0659585118147632 2202 68-69 0.03990149760846151 2202 70-71 0.04963305830965936 2202 72-73 0.04666714687222395 2202 74-75 0.033125535249538984 2202 76-77 0.033356886547082354 2202 78-79 0.06246045166334113 2202 80-81 0.08659853717218624 2202 82-83 0.028495302279395673 2202 84-85 0.10538318006689451 2202 86-87 0.0717684422151521 2202 88-89 0.11260579845399121 2202 90-91 0.12231098693963105 2202 92-93 0.05693018771653158 2202 94-95 0.09930358006268847 2202 96-97 0.05805172984983642 2202 98-99 0.03165958515198497 2202 100-101 0.05149764901249654 1102 1 -0.08775082855102312 1102 2 -0.04764206841454666 1102 3 -0.1021318478712061 1102 4 -0.14148568428375086 1102 5 -0.02362070292603846 1102 6 -0.060136155768759636 1102 7 0.09491764591715679 1102 8 0.10546862406523161 1102 9 0.09391522326934876 1102 10-11 0.03518403076876808 1102 12-13 0.14768452048784297 1102 14-15 0.08511449360036494 1102 16-17 0.0927859879057209 1102 18-19 0.08857324160134539 1102 20-21 0.13890280762596774 1102 22-23 0.14485584481322178 1102 24-25 0.14726877997814825 1102 26-27 0.09354991520729072 1102 28-29 -0.006595369444411858 1102 30-31 0.06932457687859284 1102 32-33 0.06141895448341472 1102 34-35 0.1443554760443675 1102 36-37 0.07811669594032367 1102 38-39 0.12467838689331501 1102 40-41 0.12650298297932494 1102 42-43 0.05964351268151802 1102 44-45 0.017852071674724357 1102 46-47 -0.021827022513804195 1102 48-49 -0.0303000857879141 1102 50-51 -0.003887282522384794 1102 52-53 0.002458409671653783 1102 54-55 0.007402360381533413 1102 56-57 -0.08446346710579888 1102 58-59 -0.08276030961942382 1102 60-61 -0.0556743340842587 1102 62-63 -0.11707381640103165 1102 64-65 -0.09546613187172426 1102 66-67 -0.11216576004111545 1102 68-69 -0.22038053722874196 1102 70-71 -0.11407093094579324 1102 72-73 -0.06552859186635374 1102 74-75 -0.09902254422510737 1102 76-77 -0.13829970523028834 1102 78-79 -0.16988539960844662 1102 80-81 -0.055213540273534534 1102 82-83 -0.08871029319359991 1102 84-85 -0.05971091773829329 1102 86-87 -0.05621083023631712 1102 88-89 -0.23459094856440998 1102 90-91 -0.33232759785422417 1102 92-93 -0.11507734124150204 1102 94-95 -0.10354010132589764 1102 96-97 -0.17730849802688908 1102 98-99 -0.2686609789583372 1102 100-101 -0.20313808197268912 2203 1 -0.15331705816826968 2203 2 -0.1516000880536268 2203 3 -0.10452309402189286 2203 4 -0.17893623142130366 2203 5 -0.11707568940190782 2203 6 -0.09499877001869805 2203 7 0.04291081707456357 2203 8 0.0652903173388708 2203 9 0.12954548283940426 2203 10-11 0.0760522632018521 2203 12-13 0.1221707219442294 2203 14-15 0.08054459533973812 2203 16-17 0.0678556331421376 2203 18-19 0.10453679177260966 2203 20-21 0.09677765165011465 2203 22-23 0.05680317540873148 2203 24-25 -0.014423335668020343 2203 26-27 0.052060097374379666 2203 28-29 0.019869624767565597 2203 30-31 -0.06661060984108502 2203 32-33 -0.032241985484205316 2203 34-35 -0.03089898547868586 2203 36-37 -0.047568631896368174 2203 38-39 -0.05698138525954022 2203 40-41 0.015376622009128482 2203 42-43 -0.055129318987759746 2203 44-45 -0.06196898873691481 2203 46-47 -0.1736766386367492 2203 48-49 -0.16178303362720214 2203 50-51 -0.1114141765180392 2203 52-53 -0.1103083062503103 2203 54-55 -0.15116478204590322 2203 56-57 -0.1868488798638026 2203 58-59 -0.07076494035990066 2203 60-61 -0.1788765548424891 2203 62-63 -0.14100806737676663 2203 64-65 -0.23484741782598917 2203 66-67 -0.2719457727486301 2203 68-69 -0.12651864159982296 2203 70-71 -0.12543223496824396 2203 72-73 -0.1459599289139888 2203 74-75 -0.18498803119636875 2203 76-77 -0.16907344833234106 2203 78-79 -0.20907461290861562 2203 80-81 -0.1700004971783997 2203 82-83 -0.19593768717435012 2203 84-85 -0.25746556911190766 2203 86-87 -0.3613628447031303 2203 88-89 -0.2707808043577913 2203 90-91 -0.14292770308789926 2203 92-93 -0.21246541126423324 2203 94-95 -0.13633746464098095 2203 96-97 -0.25954713632224014 2203 98-99 -0.31620577424475727 2203 100-101 -0.07446442401384701 1103 1 -0.1597409335799398 1103 2 -0.05025827902248636 1103 3 -0.12112250287633941 1103 4 -0.1993221396615681 1103 5 -0.09239081100388802 1103 6 -0.05860705900552432 1103 7 0.029047083185673728 1103 8 0.024381140917029143 1103 9 0.06515765658893002 1103 10-11 0.12766127819190842 1103 12-13 0.07484140365836822 1103 14-15 0.06050224983419383 1103 16-17 0.03963604204536608 1103 18-19 0.030510976410596413 1103 20-21 0.08248095093959762 1103 22-23 0.04653305621373249 1103 24-25 0.07051435679165508 1103 26-27 -0.010465136521311535 1103 28-29 0.0381655429142711 1103 30-31 0.047291240337166585 1103 32-33 0.01239375250425212 1103 34-35 0.02608142369329869 1103 36-37 0.031166525737425843 1103 38-39 0.016059040749503595 1103 40-41 -0.014516954932929593 1103 42-43 -0.01892938049178383 1103 44-45 -0.10516212157868665 1103 46-47 -0.18127462189188037 1103 48-49 -0.1289526129802212 1103 50-51 -0.10915157792489083 1103 52-53 -0.06956674499439686 1103 54-55 -0.03856256529893898 1103 56-57 -0.04378359926919728 1103 58-59 -0.09982359984496725 1103 60-61 -0.14323984504238751 1103 62-63 -0.1651203641221315 1103 64-65 -0.08089586560266326 1103 66-67 -0.16515189742002434 1103 68-69 -0.15588145669496356 1103 70-71 -0.06407249147065386 1103 72-73 -0.11718414865292459 1103 74-75 -0.17066296112274415 1103 76-77 -0.28694036683812385 1103 78-79 -0.2556012922547879 1103 80-81 -0.22184912855907868 1103 82-83 -0.3315940822126322 1103 84-85 -0.1797524898719658 1103 86-87 -0.38770980078728456 1103 88-89 -0.32251307986584976 1103 90-91 -0.27310706261533824 1103 92-93 -0.1896299089204092 1103 94-95 -0.21679921118511203 1103 96-97 -0.2727405775189169 1103 98-99 -0.22374659426395738 1103 100-101 -0.054310227325082394 2204 1 -0.09462257336168989 2204 2 -0.08997292141904722 2204 3 0.009929583114150375 2204 4 -0.1342422830554355 2204 5 -0.035895168558518264 2204 6 0.014409168433473951 2204 7 0.0443662494816337 2204 8 0.051905379284640674 2204 9 0.04162474448052933 2204 10-11 0.1044945514779414 2204 12-13 0.16073441787696652 2204 14-15 0.08890402104619 2204 16-17 0.053324842502000536 2204 18-19 0.12316847222733429 2204 20-21 0.1415586630893415 2204 22-23 0.09581254448010412 2204 24-25 0.09910063164052474 2204 26-27 0.10751798841122451 2204 28-29 0.1140518436384852 2204 30-31 0.08244615549514123 2204 32-33 0.11916974794294788 2204 34-35 0.042416874782595926 2204 36-37 0.1100055435675813 2204 38-39 0.13502704453431136 2204 40-41 0.05813704060753366 2204 42-43 -0.0030483307549786787 2204 44-45 -0.015435347965947699 2204 46-47 0.04775885063888552 2204 48-49 0.01441008623299922 2204 50-51 -0.028397707280532813 2204 52-53 0.019479876920989625 2204 54-55 -0.09917784356660775 2204 56-57 -0.005782396260507028 2204 58-59 0.028354559634244936 2204 60-61 -0.026716641573941047 2204 62-63 0.04854079910636955 2204 64-65 -0.0029428390926966586 2204 66-67 -0.05009241134469988 2204 68-69 -0.02718179386513242 2204 70-71 -0.008154560773945718 2204 72-73 -0.03448967017403248 2204 74-75 -0.039766135791396096 2204 76-77 -0.030714885674754555 2204 78-79 -0.011416719976054424 2204 80-81 -0.09409906392802014 2204 82-83 -0.013494800419593389 2204 84-85 0.009201264997990677 2204 86-87 -0.010703544867070036 2204 88-89 -0.008585294880106176 2204 90-91 0.017738637711509853 2204 92-93 0.031803232765781786 2204 94-95 0.01473648046008691 2204 96-97 0.02093708639818459 2204 98-99 -0.044157833181856176 2204 100-101 0.12507909707862552 2205 1 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-0.060841579858973205 1215 32-33 -0.055981686750016024 1215 34-35 -0.08604131999786091 1215 36-37 -0.08346557278295563 1215 38-39 -0.07018470431884083 1215 40-41 -0.07781064510980684 1215 42-43 0.027756516567109202 1215 44-45 0.04551296382165049 1215 46-47 0.028722272084266365 1215 48-49 0.06080899509470328 1215 50-51 0.054041488106605584 1215 52-53 0.08448315477966872 1215 54-55 0.08759750704017932 1215 56-57 0.031030100562695395 1215 58-59 0.1012730654984253 1215 60-61 0.00574153841862568 1215 62-63 -0.015719238602009966 1215 64-65 -0.0097451909767301 1215 66-67 0.046936998575354494 1215 68-69 0.07776639984226819 1215 70-71 0.09341134127701167 1215 72-73 0.13377412403023925 1215 74-75 0.18103499858104044 1215 76-77 0.16614877910947 1215 78-79 0.09478781840543604 1215 80-81 0.13612563361873242 1215 82-83 0.272924661752981 1215 84-85 0.1520599638746276 1215 86-87 0.21844037148370177 1215 88-89 0.12766733907366046 1215 90-91 0.13741699773586902 1215 92-93 0.20096900844342258 1215 94-95 0.19395121520395975 1215 96-97 0.1780390528458753 1215 98-99 0.2204342669929531 1215 100-101 0.15131642370732123 1212 1 0.22096972671638326 1212 2 0.07767907088879156 1212 3 0.08955642437614841 1212 4 0.1374154029186201 1212 5 0.07031164362128806 1212 6 -0.0910133467656351 1212 7 -0.1548013326925357 1212 8 -0.25847965646890714 1212 9 -0.2390275738300076 1212 10-11 -0.24097125401138442 1212 12-13 -0.29107322390663626 1212 14-15 -0.27209303439051524 1212 16-17 -0.23193743973528314 1212 18-19 -0.1689922248849669 1212 20-21 -0.13821958662546763 1212 22-23 -0.21217276698230592 1212 24-25 -0.10543836789244665 1212 26-27 -0.11349330662350354 1212 28-29 -0.06869047738448586 1212 30-31 -0.09111322194997484 1212 32-33 -0.10367503929778366 1212 34-35 -0.04225279528105119 1212 36-37 -0.06423176303842126 1212 38-39 -0.03232698206622331 1212 40-41 -0.09264963851253327 1212 42-43 0.08275494973072028 1212 44-45 0.07074226793137939 1212 46-47 0.04279748720117027 1212 48-49 0.1395426616603075 1212 50-51 -0.058054060451681266 1212 52-53 -0.013373415942588451 1212 54-55 0.04220485551736175 1212 56-57 0.09831108616813111 1212 58-59 0.09555461365476248 1212 60-61 0.0881221364825393 1212 62-63 0.09791432904524555 1212 64-65 0.0830353235612975 1212 66-67 0.13347512947748186 1212 68-69 0.09052874691961676 1212 70-71 0.06469592784711864 1212 72-73 0.1416141798032271 1212 74-75 0.18124022655791094 1212 76-77 0.18131018649157227 1212 78-79 0.18610896787959064 1212 80-81 0.09323848272266133 1212 82-83 0.0726518666795073 1212 84-85 -0.010969936126656421 1212 86-87 0.012560683607965473 1212 88-89 -0.0023543788179480885 1212 90-91 0.014591741959627313 1212 92-93 0.1273942051474677 1212 94-95 0.03766232019681581 1212 96-97 0.07538200908132353 1212 98-99 0.14417430690880906 1212 100-101 -0.05556985853117169 1213 1 -0.0713576621829688 1213 2 0.06996655758687353 1213 3 0.025691407498143803 1213 4 0.12177513568625642 1213 5 -0.008130817871062845 1213 6 -0.02485882393936123 1213 7 -0.10158378106320498 1213 8 -0.34476992954561325 1213 9 -0.3398419073660719 1213 10-11 -0.36582396782561943 1213 12-13 -0.24508624731974749 1213 14-15 -0.19703527357059158 1213 16-17 -0.21587683009699532 1213 18-19 -0.17642854273661612 1213 20-21 -0.2392139446124375 1213 22-23 -0.26811646053373295 1213 24-25 -0.36369842987048884 1213 26-27 -0.26457152820491103 1213 28-29 -0.28028542821227376 1213 30-31 -0.46884073417897554 1213 32-33 -0.36974105510619637 1213 34-35 -0.39963582432105227 1213 36-37 -0.4264888571203471 1213 38-39 -0.5846476206572433 1213 40-41 -0.4153548673805645 1213 42-43 -0.1517831541937653 1213 44-45 -0.3129648782303178 1213 46-47 -0.3074181327721277 1213 48-49 -0.21908170112669723 1213 50-51 -0.4091789312969496 1213 52-53 -0.3384021984734815 1213 54-55 -0.1467222369737513 1213 56-57 -0.1781315224369493 1213 58-59 -0.26445753836082275 1213 60-61 -0.16960615715611027 1213 62-63 -0.3156324784277089 1213 64-65 -0.3254023833413484 1213 66-67 -0.17689095932335164 1213 68-69 -0.1762056946603323 1213 70-71 -0.20647885990137382 1213 72-73 -0.10259043625056563 1213 74-75 -0.18143428877233703 1213 76-77 -0.23143193537968898 1213 78-79 -0.18653512968009522 1213 80-81 -0.13011999812609076 1213 82-83 -0.106002139365593 1213 84-85 -0.043272952834506384 1213 86-87 -0.025404700918009837 1213 88-89 -0.12532515713970582 1213 90-91 -0.11396517819576246 1213 92-93 -0.17662929588993137 1213 94-95 -0.22079565020804637 1213 96-97 -0.17181202633681636 1213 98-99 -0.11702342125825282 1213 100-101 -0.8847818442192832 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37.0 3 54.0 4 66.0 5 92.0 6 93.0 7 64.0 8 60.0 9 51.0 10 70.0 11 56.0 12 57.0 13 109.0 14 509.0 15 3825.0 16 15560.0 17 28675.0 18 33479.0 19 34040.0 20 36170.0 21 39518.0 22 45432.0 23 51819.0 24 59746.0 25 67753.0 26 77168.0 27 87439.0 28 99948.0 29 115201.0 30 132995.0 31 155718.0 32 185687.0 33 224157.0 34 280100.0 35 370618.0 36 534310.0 37 912071.0 38 2237193.0 39 4303022.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.852558884103713 34.397354442893175 33.28379509426361 16.4662915787395 2 17.09550484011471 35.6079160194775 31.851230262231393 15.44534887817639 3 17.39437143744218 33.50991797148632 32.47375014531989 16.621960445751615 4 17.398679905964446 32.73571088162232 32.74349750408649 17.12211170832674 5 17.62301913902468 32.945914739023934 32.99191740719842 16.43914871475296 6 17.645027890149706 33.50804527725021 31.67481844309065 17.17210838950943 7 17.75945944772199 33.266956612029496 32.51156484823074 16.462019092017776 8 17.232405975009314 33.505853867583426 32.6139116184531 16.647828538954162 9 17.214476355599032 32.948115786527524 32.92253528076776 16.914872577105683 10-11 17.308018377619984 33.25651647144328 32.488854481953005 16.946610668983737 12-13 17.109847048114172 33.28304651091866 32.781155400691226 16.825951040275942 14-15 17.263868167722364 33.10920167875384 32.692371196611894 16.934558956911903 16-17 17.356915651786228 32.9831084058819 32.75510638169769 16.90486956063418 18-19 17.296895095877332 32.841725200199015 32.69374477055285 17.167634933370802 20-21 17.490650874070603 32.71934480164712 32.53518032138666 17.25482400289562 22-23 17.436391835591984 32.78579756839944 32.45753573762193 17.320274858386647 24-25 17.663641877252896 32.579292877243816 32.39829118449893 17.35877406100436 26-27 17.750767442152 32.667223125359854 32.230443193092384 17.351566239395755 28-29 17.70210239202992 32.697975989867615 32.224007368293236 17.375914249809227 30-31 17.81101329220395 32.49040602006415 32.253300320706735 17.44528036702517 32-33 17.812580630018253 32.65248751519676 32.14528629046211 17.38964556432288 34-35 17.887398487857237 32.57583070823628 32.12092246275659 17.415848341149896 36-37 17.84258016903295 32.574992379144284 32.071458157948356 17.51096929387441 38-39 17.86486882953091 32.55742039702966 32.13500777490754 17.44270299853189 40-41 17.96051977560197 32.621744479760906 32.01184361320827 17.405892131428843 42-43 17.86489255906899 32.515324128506364 32.092951093404345 17.526832219020292 44-45 17.910037797179324 32.502968128416235 31.986340538281823 17.600653536122618 46-47 17.94349563590299 32.63709819323151 31.928487396845195 17.490918774020304 48-49 17.90109558184092 32.44136875178295 32.02461530261019 17.63292036376594 50-51 17.983108798172275 32.498173367806274 31.8511146430004 17.667603191021055 52-53 17.95651540840225 32.61284646385374 31.950093904013073 17.480544223730938 54-55 18.04172157276028 32.388121754284185 31.92186357562697 17.64829309732857 56-57 18.08536954451795 32.55448547544257 31.804897449266562 17.55524753077292 58-59 17.978529355571204 32.47310715584664 31.97037462304808 17.577988865534078 60-61 18.038616975723404 32.43207970689896 31.79028812800095 17.73901518937669 62-63 17.958890667263702 32.45475299667015 31.88390109787868 17.70245523818747 64-65 17.96133116415339 32.48369672329348 31.904876908613545 17.650095203939586 66-67 18.048602614015916 32.50076538636353 31.73666227580275 17.7139697238178 68-69 17.935961995249407 32.43790356911497 31.868187679304068 17.757946756331556 70-71 18.118070414844333 32.48149391797941 31.63433532769387 17.766100339482392 72-73 18.022939352665386 32.49121148804882 31.76101605302532 17.724833106260473 74-75 18.0654240251378 32.42207988043158 31.775199128508902 17.73729696592172 76-77 18.064636664331914 32.527922034010416 31.746638175752217 17.66080312590546 78-79 18.08881314686316 32.42939311633325 31.828499431729135 17.653294305074454 80-81 18.16682948549415 32.43843695900174 31.679253656265843 17.715479899238264 82-83 18.102683760239056 32.65147419745346 31.734089154061085 17.511752888246402 84-85 18.0589621094122 32.3691788286872 31.78215149590273 17.789707565997873 86-87 18.05296421765879 32.59050516899812 31.662530701316786 17.693999912026296 88-89 18.06985026035817 32.455289555587655 31.821369719856644 17.65349046419753 90-91 18.120902185438563 32.35667405589383 31.69535122460371 17.82707253406389 92-93 18.060982948311747 32.51215186607549 31.72630231219315 17.700562873419614 94-95 18.07280581365905 32.48850561972987 31.672419308041 17.766269258570077 96-97 18.07463566460492 32.53620347477723 31.67224623008576 17.716914630532088 98-99 18.102148677208266 32.501692057443606 31.662789996725245 17.73336926862289 100-101 18.24437384477133 32.460952240451775 31.47392279501634 17.82075111976055 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1075.0 1 1218.0 2 1433.5 3 1705.5 4 2076.5 5 2550.0 6 3112.5 7 3832.0 8 4869.0 9 6468.5 10 8699.5 11 11941.5 12 17263.5 13 24909.5 14 36470.5 15 51776.5 16 71930.5 17 97450.5 18 125734.5 19 156125.0 20 186837.5 21 216623.5 22 243947.5 23 266388.0 24 282052.5 25 291652.0 26 294361.0 27 291478.0 28 289928.5 29 289439.0 30 290674.5 31 294413.0 32 298038.0 33 302013.5 34 304323.5 35 309208.5 36 324744.5 37 343212.5 38 356778.5 39 360013.0 40 351625.5 41 332331.5 42 302525.0 43 268242.5 44 234412.0 45 207602.5 46 188624.5 47 178671.5 48 171261.5 49 160576.0 50 148765.0 51 136389.0 52 123408.5 53 111337.0 54 101382.0 55 93212.5 56 85838.5 57 78085.5 58 70223.0 59 61678.0 60 52605.5 61 43265.0 62 35011.0 63 28111.0 64 22268.5 65 17828.0 66 14274.0 67 11000.5 68 8273.0 69 6134.5 70 4598.5 71 3606.0 72 2821.0 73 2219.0 74 1747.0 75 1382.0 76 1135.5 77 927.0 78 742.0 79 610.0 80 475.0 81 370.0 82 327.5 83 264.5 84 165.5 85 99.0 86 67.0 87 43.0 88 26.5 89 21.5 90 14.5 91 12.0 92 12.5 93 9.5 94 7.0 95 8.0 96 5.5 97 7.0 98 9.5 99 9.0 100 15.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.835703518872369E-4 2 4.0462009035462684E-4 3 0.0014211047075869821 4 0.0019638877556236766 5 0.009286524512773264 6 0.014092621683570904 7 0.013796558202823616 8 0.010145108606940399 9 0.002427720542127761 10-11 0.006296283357225656 12-13 0.010687891654977093 14-15 0.03379564632730291 16-17 0.014536716904691837 18-19 0.02201231979356086 20-21 0.01777367762752885 22-23 0.03050440729966223 24-25 0.026349649786508625 26-27 0.022017254184906642 28-29 0.030316900428522284 30-31 0.03259165483893061 32-33 0.03698326313668205 34-35 0.01882963737552751 36-37 0.02828393119405757 38-39 0.028994483547851065 40-41 0.032399213576444874 42-43 0.03948499954899663 44-45 0.028037211626768165 46-47 0.028219784106562326 48-49 0.02242187427526127 50-51 0.03616908856462701 52-53 0.028984614765159487 54-55 0.03124950039287624 56-57 0.02431668055204391 58-59 0.024923610687575853 60-61 0.0330554876254347 62-63 0.028880992546897934 64-65 0.028288865585403358 66-67 0.024252533464548667 68-69 0.02614733974133131 70-71 0.02942377559493463 72-73 0.03639113617518747 74-75 0.031550498264969314 76-77 0.03372163045711609 78-79 0.03502924416374995 80-81 0.040629778341219475 82-83 0.042253193093983774 84-85 0.04750338548590234 86-87 0.04870737697427465 88-89 0.05114990069043977 90-91 0.05087357477507563 92-93 0.051283129256776055 94-95 0.052260138743242104 96-97 0.05306937892395135 98-99 0.05426350162963208 100-101 0.058309702533178354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0132962E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.910986226006514 #Duplication Level Percentage of deduplicated Percentage of total 1 31.559507793146903 9.439760029003622 2 17.808777901727566 10.65356221041165 3 14.93692318616613 13.403343110409974 4 12.302795081081651 14.71954936846456 5 8.100718475872975 12.115023937629655 6 5.500085751592707 9.87077934946465 7 3.3312065838788985 6.974777197246877 8 1.9596497338842782 4.689204495840798 9 1.2825534904994143 3.4526195809600098 >10 3.1818786701245814 13.844254430826025 >50 0.03266505107156294 0.6402015776557559 >100 0.002967802957127311 0.15033134861563302 >500 2.7047799615231567E-4 0.046593363470801515 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.960634807472879E-5 0.0 0.0 2 2.960634807472879E-5 0.0 0.0 3 2.960634807472879E-5 0.0 0.0 4 2.960634807472879E-5 0.0 0.0 5 2.960634807472879E-5 0.0 0.0 6 2.960634807472879E-5 0.0 0.0 7 3.9475130766305056E-5 0.0 0.0 8 3.9475130766305056E-5 0.0 0.0 9 3.9475130766305056E-5 0.0 1.9737565383152528E-5 10-11 3.9475130766305056E-5 0.0 3.9475130766305056E-5 12-13 4.4409522112093185E-5 0.0 5.921269614945758E-5 14-15 1.529661317194321E-4 0.0 7.895026153261011E-5 16-17 1.9737565383152528E-4 0.0 7.895026153261011E-5 18-19 2.1217882786888967E-4 0.0 1.1349100095312702E-4 20-21 2.763259153641354E-4 0.0 1.5790052306522022E-4 22-23 4.095544817004149E-4 0.0 1.5790052306522022E-4 24-25 5.921269614945759E-4 0.0 1.5790052306522022E-4 26-27 8.092401807092537E-4 0.0 1.5790052306522022E-4 28-29 9.523375297371095E-4 0.0 1.5790052306522022E-4 30-31 0.0011447787922228467 0.0 1.5790052306522022E-4 32-33 0.0013421544460543717 1.9737565383152528E-5 1.9737565383152528E-4 34-35 0.0015839396219979904 1.9737565383152528E-5 1.9737565383152528E-4 36-37 0.0017418401450632105 4.440952211209319E-5 1.9737565383152528E-4 38-39 0.0020082972777357697 6.908147884103385E-5 2.0724443652310156E-4 40-41 0.002368507845978303 6.908147884103385E-5 2.171132192146778E-4 42-43 0.002728718414220837 7.895026153261011E-5 2.467195672894066E-4 44-45 0.0030987977651549467 7.895026153261011E-5 2.467195672894066E-4 46-47 0.0032863046362948956 1.233597836447033E-4 2.467195672894066E-4 48-49 0.003582368117042184 1.4803174037364395E-4 2.467195672894066E-4 50-51 0.003858694032406319 1.4803174037364395E-4 2.5658834998098286E-4 52-53 0.004154757513153607 1.727036971025846E-4 2.6645713267255914E-4 54-55 0.004505099298704565 1.87506871139949E-4 2.763259153641354E-4 56-57 0.004865309866947097 1.87506871139949E-4 2.763259153641354E-4 58-59 0.0051416357823112335 1.87506871139949E-4 2.763259153641354E-4 60-61 0.005432764871712733 1.87506871139949E-4 2.763259153641354E-4 62-63 0.005773237874572115 2.0724443652310153E-4 2.812603067099235E-4 64-65 0.0062025299216556815 2.0724443652310153E-4 3.35538611513593E-4 66-67 0.0065331341418234865 2.0724443652310153E-4 3.6514495958832175E-4 68-69 0.00691801666679496 2.0724443652310153E-4 3.6514495958832175E-4 70-71 0.007347308713878528 2.0724443652310153E-4 3.6514495958832175E-4 72-73 0.007781535152307884 2.0724443652310153E-4 3.6514495958832175E-4 74-75 0.008255236721503544 2.171132192146778E-4 3.6514495958832175E-4 76-77 0.008798019769540238 2.2698200190625407E-4 4.046200903546268E-4 78-79 0.00964180068967001 2.2698200190625407E-4 4.046200903546268E-4 80-81 0.010293140347314043 2.2698200190625407E-4 4.194232643919912E-4 82-83 0.011003692701107534 2.2698200190625407E-4 4.342264384293556E-4 84-85 0.011896817534695186 2.2698200190625407E-4 5.082423086161776E-4 86-87 0.012878761412507024 2.2698200190625407E-4 5.62520613419847E-4 88-89 0.014245587815290336 2.2698200190625407E-4 5.62520613419847E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATCT 545 0.0 10.895328 32-33 TCCGATC 600 1.0186341E-10 8.708999 32-33 TGCGGGC 705 0.008180869 6.735892 3 CGGGCTC 900 0.0018576346 6.331769 5 AAGCTAG 3360 0.0 6.2186713 1 TCAGACG 1410 1.8189894E-12 5.8956366 18-19 CGATCTA 725 7.607509E-5 5.569322 34-35 CAGACGT 1160 9.422365E-9 5.5282483 18-19 >>END_MODULE