FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 11-60308.34200000001be3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 11-60308.34200000001be3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10132962
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT5450.010.89532832-33
TCCGATC6001.0186341E-108.70899932-33
TGCGGGC7050.0081808696.7358923
CGGGCTC9000.00185763466.3317695
AAGCTAG33600.06.21867131
TCAGACG14101.8189894E-125.895636618-19
CGATCTA7257.607509E-55.56932234-35
CAGACGT11609.422365E-95.528248318-19
Produced by FastQC (version 0.11.2)