##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n02 10-60155.34200000001bc9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9680571 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.14065027775737 38.0 32.0 38.0 32.0 38.0 2 35.35465232371107 38.0 32.0 38.0 32.0 38.0 3 35.382456262135776 38.0 32.0 38.0 32.0 38.0 4 35.50084876191704 38.0 32.0 38.0 32.0 38.0 5 35.197567478199375 38.0 32.0 38.0 32.0 38.0 6 37.57414609117582 40.0 38.0 40.0 32.0 40.0 7 37.722819139490845 40.0 38.0 40.0 32.0 40.0 8 37.81207906021246 40.0 38.0 40.0 32.0 40.0 9 37.840139904970485 40.0 38.0 40.0 32.0 40.0 10-11 37.84857205220642 40.0 38.0 40.0 32.0 40.0 12-13 37.82511072952205 40.0 38.0 40.0 32.0 40.0 14-15 37.78466017138865 40.0 38.0 40.0 32.0 40.0 16-17 37.77894604564132 40.0 38.0 40.0 32.0 40.0 18-19 37.75370249337565 40.0 38.0 40.0 32.0 40.0 20-21 37.73363203472192 40.0 38.0 40.0 32.0 40.0 22-23 37.720006702084 40.0 38.0 40.0 32.0 40.0 24-25 37.66560701842897 40.0 38.0 40.0 32.0 40.0 26-27 37.64575044178696 40.0 38.0 40.0 32.0 40.0 28-29 37.57264106631726 40.0 38.0 40.0 32.0 40.0 30-31 37.53367115431517 40.0 38.0 40.0 32.0 40.0 32-33 37.5365031670136 40.0 38.0 40.0 32.0 40.0 34-35 37.461450259493986 40.0 38.0 40.0 32.0 40.0 36-37 37.46472961150742 40.0 38.0 40.0 32.0 40.0 38-39 37.41907708749825 40.0 38.0 40.0 32.0 40.0 40-41 37.323409590198764 40.0 38.0 40.0 32.0 40.0 42-43 37.103820528768395 39.0 38.0 40.0 32.0 40.0 44-45 37.01540301703278 38.0 38.0 40.0 32.0 40.0 46-47 36.93966879639641 38.0 38.0 40.0 32.0 40.0 48-49 36.85899023931543 38.0 38.0 40.0 32.0 40.0 50-51 36.79400961988709 38.0 38.0 40.0 32.0 40.0 52-53 36.72486245904297 38.0 38.0 40.0 32.0 40.0 54-55 36.67579541537374 38.0 38.0 40.0 32.0 40.0 56-57 36.64922410052051 38.0 38.0 40.0 32.0 40.0 58-59 36.58169419965 38.0 38.0 40.0 32.0 40.0 60-61 36.54751052391434 38.0 38.0 40.0 32.0 40.0 62-63 36.467246560146094 38.0 38.0 40.0 32.0 40.0 64-65 36.448839071579556 38.0 38.0 40.0 32.0 40.0 66-67 36.38633712825411 38.0 38.0 40.0 27.0 40.0 68-69 36.330418164383076 38.0 38.0 40.0 27.0 40.0 70-71 36.21784081744765 38.0 38.0 40.0 27.0 40.0 72-73 36.15000008780474 38.0 38.0 40.0 27.0 40.0 74-75 36.05656598149014 38.0 38.0 40.0 27.0 40.0 76-77 35.97151547155638 38.0 38.0 40.0 27.0 40.0 78-79 35.884479283298475 38.0 38.0 40.0 27.0 40.0 80-81 35.80515896221411 38.0 38.0 40.0 27.0 40.0 82-83 35.714900391722765 38.0 38.0 40.0 27.0 40.0 84-85 35.61243629120638 38.0 38.0 40.0 27.0 40.0 86-87 35.54152456502824 38.0 38.0 40.0 27.0 40.0 88-89 35.47379142201426 38.0 38.0 40.0 27.0 40.0 90-91 35.34920026928164 38.0 38.0 40.0 27.0 40.0 92-93 35.24582382588795 38.0 38.0 40.0 27.0 40.0 94-95 35.14691808985235 38.0 38.0 40.0 27.0 40.0 96-97 35.05966585028921 38.0 38.0 40.0 27.0 40.0 98-99 34.91615866460769 38.0 35.0 40.0 27.0 40.0 100-101 32.927497148670255 38.0 29.5 39.0 20.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.2038877278376603 2201 2 0.3571132932895722 2201 3 0.446746267385727 2201 4 0.4214025596581763 2201 5 0.4322667091550798 2201 6 0.45890696316335067 2201 7 0.38162135865862723 2201 8 0.28206146483773153 2201 9 0.3033186446498206 2201 10-11 0.2680456882406759 2201 12-13 0.3172250702100854 2201 14-15 0.30236107491178643 2201 16-17 0.24199748065574056 2201 18-19 0.23937103543237725 2201 20-21 0.2768409246354935 2201 22-23 0.2707718451036527 2201 24-25 0.2723303511004431 2201 26-27 0.2895651289628347 2201 28-29 0.286227780874043 2201 30-31 0.259913746183571 2201 32-33 0.291109356439577 2201 34-35 0.26675293375183173 2201 36-37 0.1637181104489258 2201 38-39 0.2516968206129775 2201 40-41 0.26219876958101906 2201 42-43 0.27070950701511265 2201 44-45 0.22565563100088326 2201 46-47 0.2918968560071207 2201 48-49 0.16014588404933505 2201 50-51 0.2726803730531415 2201 52-53 0.24602054073486812 2201 54-55 0.3723771883702227 2201 56-57 0.1913419701161203 2201 58-59 0.21798718397035088 2201 60-61 0.12399339932576225 2201 62-63 0.22719342523920716 2201 64-65 0.13439862670467306 2201 66-67 0.1989991109919842 2201 68-69 0.07295438621962802 2201 70-71 0.26291131553151814 2201 72-73 0.010888646669805269 2201 74-75 0.21394038575770224 2201 76-77 0.14437865665990302 2201 78-79 0.22615228671089227 2201 80-81 0.23964505301979955 2201 82-83 0.19421227054878187 2201 84-85 0.1129345748406223 2201 86-87 0.23699729806138237 2201 88-89 0.17693875863778175 2201 90-91 0.07817709056593714 2201 92-93 0.06980708158329918 2201 94-95 0.17767742601334646 2201 96-97 -0.0829452409571445 2201 98-99 0.0418643854358578 2201 100-101 0.2263540232643635 1101 1 0.10091808838387806 1101 2 0.13933710310245573 1101 3 0.30969832302046996 1101 4 0.19257738526677315 1101 5 0.23963084041256622 1101 6 0.2878278057021504 1101 7 0.2992152705646518 1101 8 0.2722235010299343 1101 9 0.3212832760168425 1101 10-11 0.31338296866277204 1101 12-13 0.286120119123197 1101 14-15 0.26010115069665574 1101 16-17 0.29340605722414637 1101 18-19 0.277629824639277 1101 20-21 0.30313661251278745 1101 22-23 0.31871416837852706 1101 24-25 0.2792021526722266 1101 26-27 0.276694101125905 1101 28-29 0.2549686064012704 1101 30-31 0.27105946110401646 1101 32-33 0.20896364389066946 1101 34-35 0.26767250539679566 1101 36-37 0.25977009771892057 1101 38-39 0.23673647460116598 1101 40-41 0.25466331220045646 1101 42-43 0.23042833530545437 1101 44-45 0.18205402739059195 1101 46-47 0.26524770540148523 1101 48-49 0.19897494005061844 1101 50-51 0.24214261193110787 1101 52-53 0.2821416387090281 1101 54-55 0.1667268788870615 1101 56-57 0.20396799423605927 1101 58-59 0.14921633240588505 1101 60-61 0.11286698927776939 1101 62-63 0.17289760603456727 1101 64-65 0.18329397560096794 1101 66-67 0.15758352239372186 1101 68-69 0.09559011913813009 1101 70-71 0.19907783293036374 1101 72-73 0.19033023259895998 1101 74-75 0.21933403406659835 1101 76-77 0.158365951479027 1101 78-79 0.09072324131066267 1101 80-81 0.23414249295286282 1101 82-83 0.2397441827687743 1101 84-85 0.2508012669350208 1101 86-87 0.30069373701155655 1101 88-89 0.17156860695808973 1101 90-91 0.08871229967500227 1101 92-93 0.15717022850172668 1101 94-95 0.1001056839115364 1101 96-97 0.07597184994394723 1101 98-99 0.1427665771517752 1101 100-101 0.2259758288631275 2202 1 -0.012743036709778721 2202 2 0.007643865107013426 2202 3 0.13751207050977854 2202 4 0.11927435952824084 2202 5 0.05006549662577697 2202 6 0.2009379035978185 2202 7 0.252434594436167 2202 8 0.1538593791791243 2202 9 0.2204172562609088 2202 10-11 0.20175756342979412 2202 12-13 0.19187151178778095 2202 14-15 0.17502339091971209 2202 16-17 0.1801765262652708 2202 18-19 0.2042507023308957 2202 20-21 0.17114782730113376 2202 22-23 0.2342730570572087 2202 24-25 0.19411838173509466 2202 26-27 0.21280501808619334 2202 28-29 0.2035855256378909 2202 30-31 0.2096370709364095 2202 32-33 0.22629147755889534 2202 34-35 0.1878518739973103 2202 36-37 0.2103170323709307 2202 38-39 0.1676928620125011 2202 40-41 0.18056353748846732 2202 42-43 0.11948832775318863 2202 44-45 0.016055220628281575 2202 46-47 -0.03997780122748651 2202 48-49 0.058343074005954065 2202 50-51 0.1439134310409358 2202 52-53 0.13838466215741363 2202 54-55 0.12023001134055988 2202 56-57 0.1367263978723443 2202 58-59 0.11678154538648045 2202 60-61 0.05395830325640105 2202 62-63 0.18031963300029474 2202 64-65 0.10549145760390388 2202 66-67 0.07946337743953791 2202 68-69 0.0013815443975957464 2202 70-71 0.04366910476519337 2202 72-73 -0.006456285373879211 2202 74-75 0.029318802867670968 2202 76-77 -0.03894048855070764 2202 78-79 0.021473505352282984 2202 80-81 0.05406866658888987 2202 82-83 0.02672505973631445 2202 84-85 0.014825209592096655 2202 86-87 0.05774405300238783 2202 88-89 0.11765277625627846 2202 90-91 0.08133333328053283 2202 92-93 0.12476283063347182 2202 94-95 0.09345789019754847 2202 96-97 0.08321737037391586 2202 98-99 0.04405849522186145 2202 100-101 0.1054940466471237 1102 1 -0.14458259216210223 1102 2 -0.045421915794193524 1102 3 -0.11585387487515675 1102 4 -0.0357455711811383 1102 5 -0.15288181319237992 1102 6 -0.0720410716401716 1102 7 0.12167478643845442 1102 8 0.11271506298174927 1102 9 0.1480617331742735 1102 10-11 0.07273761118043609 1102 12-13 0.20580742004877095 1102 14-15 0.15316907529360435 1102 16-17 0.12118037334859366 1102 18-19 0.16003547333714607 1102 20-21 0.1947376530955225 1102 22-23 0.155066647711827 1102 24-25 0.20334755698721096 1102 26-27 0.15803410497036197 1102 28-29 0.0038325196005004614 1102 30-31 0.09124685384249887 1102 32-33 0.08173742721048427 1102 34-35 0.13529873354290345 1102 36-37 0.08322949204629992 1102 38-39 0.11361030639706371 1102 40-41 0.134786236886697 1102 42-43 0.027096471271590872 1102 44-45 -0.037378510520589714 1102 46-47 0.011143684936470777 1102 48-49 -0.03509535377539663 1102 50-51 3.8550721252050835E-4 1102 52-53 0.03502707790342896 1102 54-55 0.025579363445110914 1102 56-57 -0.01164388216999157 1102 58-59 -0.008822196623057721 1102 60-61 0.007774247551424196 1102 62-63 -0.03811878010003511 1102 64-65 -0.09971803787848188 1102 66-67 -0.1531025076616359 1102 68-69 -0.23935263066755397 1102 70-71 -0.060116983045780614 1102 72-73 -0.015100005287202123 1102 74-75 -0.010296597323133483 1102 76-77 -0.025320075275246268 1102 78-79 -0.0539839955558179 1102 80-81 -0.018891132896179386 1102 82-83 -0.04450103828137486 1102 84-85 0.054042542903637525 1102 86-87 0.04690087646040553 1102 88-89 -0.12134849011622606 1102 90-91 -0.25331436772439275 1102 92-93 -0.10119294857930328 1102 94-95 -0.020678694014911514 1102 96-97 -0.02226944241134987 1102 98-99 -0.14805928258849121 1102 100-101 -0.019589872963685195 2203 1 -0.16465166022032207 2203 2 -0.18240513804347103 2203 3 -0.09296810799484234 2203 4 -0.09655055916555 2203 5 -0.3071913314008725 2203 6 -0.17138562934786705 2203 7 6.212866755319624E-4 2203 8 0.07656593984611249 2203 9 0.059866900024395875 2203 10-11 0.10049926495187833 2203 12-13 0.16657554337833602 2203 14-15 0.11724623151609137 2203 16-17 0.11496548997674694 2203 18-19 0.12542854474240528 2203 20-21 0.19364569881132354 2203 22-23 0.0815190903214642 2203 24-25 0.11841085391752415 2203 26-27 0.1041979875114123 2203 28-29 0.07788949535409984 2203 30-31 0.10221638703421121 2203 32-33 0.06020296071511666 2203 34-35 0.045035117115098444 2203 36-37 0.015420162252816283 2203 38-39 0.03907983692598549 2203 40-41 0.07443768128241857 2203 42-43 -0.0022066941281480013 2203 44-45 -0.03093878821970719 2203 46-47 -0.13518942564110148 2203 48-49 -0.11287393344941421 2203 50-51 -0.017137645475621355 2203 52-53 -0.039443920784279385 2203 54-55 -0.08752919431510975 2203 56-57 -0.17178800473266875 2203 58-59 -0.04809054312156036 2203 60-61 -0.1309431939306549 2203 62-63 -0.15165525433548055 2203 64-65 -0.21051308862382 2203 66-67 -0.14292401106650487 2203 68-69 -0.07584636297610103 2203 70-71 -0.14121561977319885 2203 72-73 -0.12450952967519413 2203 74-75 -0.07800876988778072 2203 76-77 -0.1129769167693695 2203 78-79 -0.17694676398753018 2203 80-81 -0.17281637217039503 2203 82-83 -0.24288204534123992 2203 84-85 -0.24495380742781236 2203 86-87 -0.38732245726041015 2203 88-89 -0.2602705873038502 2203 90-91 -0.09519341676481474 2203 92-93 -0.19413710597855527 2203 94-95 -0.11470580732065372 2203 96-97 -0.2156504905607477 2203 98-99 -0.27653759172353887 2203 100-101 0.0033012596584427456 1103 1 -0.2831250063261308 1103 2 -0.10568112460359913 1103 3 -0.11215935797867616 1103 4 -0.03379003751636134 1103 5 -0.21357246673603214 1103 6 -0.09940578625552376 1103 7 0.07467635985325671 1103 8 0.02809719795595811 1103 9 0.11422449709903049 1103 10-11 0.15143957171830635 1103 12-13 0.09014713101169036 1103 14-15 0.08916427806144611 1103 16-17 0.06387270486715835 1103 18-19 0.0788514187181022 1103 20-21 0.09308609124678924 1103 22-23 0.04553959690134235 1103 24-25 0.09628733698844627 1103 26-27 0.052978117865627894 1103 28-29 0.021587663277543356 1103 30-31 0.0779535532450808 1103 32-33 0.07577479294742773 1103 34-35 0.06020378347393063 1103 36-37 0.06660967153160868 1103 38-39 0.107212378012143 1103 40-41 0.04139443135561294 1103 42-43 -0.05325423569082943 1103 44-45 -0.1407839160276083 1103 46-47 -0.11311123475993412 1103 48-49 -0.11217450649474614 1103 50-51 -0.1052765793990531 1103 52-53 -0.06162106336157791 1103 54-55 -0.014857893864430594 1103 56-57 -0.038376021548621964 1103 58-59 -0.05871017049162219 1103 60-61 -0.18889029389519862 1103 62-63 -0.13854135781469523 1103 64-65 -0.15536415848355034 1103 66-67 -0.27844778877793175 1103 68-69 -0.213484410244817 1103 70-71 -0.1610886579984836 1103 72-73 -0.16550079367759452 1103 74-75 -0.2020234934777605 1103 76-77 -0.28756983374854883 1103 78-79 -0.26182959805122863 1103 80-81 -0.2093597269064631 1103 82-83 -0.2683159147858518 1103 84-85 -0.1992072354432608 1103 86-87 -0.3249810076769677 1103 88-89 -0.2717306358329097 1103 90-91 -0.26032665389538323 1103 92-93 -0.24046215845579155 1103 94-95 -0.2174692805255134 1103 96-97 -0.2845433938381632 1103 98-99 -0.24702488947399104 1103 100-101 -0.25314088021414705 2204 1 -0.08890542557256254 2204 2 -0.05938145681629692 2204 3 0.022566055456636036 2204 4 -0.02786247032924649 2204 5 -0.13282306128648003 2204 6 -0.01050591430807657 2204 7 0.09613348445691372 2204 8 0.09423443630053185 2204 9 0.07929106520562357 2204 10-11 0.16848882178548052 2204 12-13 0.16344719855588608 2204 14-15 0.1200531003138039 2204 16-17 0.08195162575472636 2204 18-19 0.13078874121065098 2204 20-21 0.17798640688890544 2204 22-23 0.16076512706127488 2204 24-25 0.1470434946789183 2204 26-27 0.18440754226923417 2204 28-29 0.16843251482330857 2204 30-31 0.16560310043284687 2204 32-33 0.13459551070336317 2204 34-35 0.103230912482978 2204 36-37 0.13595523091751716 2204 38-39 0.20050372566300467 2204 40-41 0.09169373068414188 2204 42-43 0.018434745737877734 2204 44-45 0.028125309870262072 2204 46-47 0.09672582564601129 2204 48-49 0.05798768520252651 2204 50-51 0.01210872974123589 2204 52-53 0.10392168005542857 2204 54-55 0.004950350255725766 2204 56-57 0.03409054112439236 2204 58-59 0.09627382843976307 2204 60-61 0.09292713919600004 2204 62-63 0.07104993542555604 2204 64-65 0.037427078980435624 2204 66-67 0.03725795089354733 2204 68-69 0.06023652903052579 2204 70-71 0.0332580039826027 2204 72-73 0.10224273592038458 2204 74-75 0.052443410112481104 2204 76-77 0.0556903195392664 2204 78-79 -4.7800524519914234E-5 2204 80-81 0.04957484819912139 2204 82-83 0.05272641403460909 2204 84-85 -0.0029932546070980948 2204 86-87 0.011018343696122201 2204 88-89 0.061024937632588205 2204 90-91 0.11384065585667003 2204 92-93 0.09621010026224042 2204 94-95 0.057522784940225336 2204 96-97 0.04212118189296632 2204 98-99 -0.021365295252259386 2204 100-101 0.15808314840314353 2205 1 -0.05544818490816539 2205 2 0.0791817932799006 2205 3 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0.01720453403974176 1215 48-49 0.014588423094743064 1215 50-51 -0.056250205508483475 1215 52-53 -0.008556472134024773 1215 54-55 -0.04355638460808109 1215 56-57 -0.023429668381957924 1215 58-59 0.02265444173075082 1215 60-61 -0.08500478844501203 1215 62-63 -0.0477767909682143 1215 64-65 -0.14102342109144672 1215 66-67 -0.05573320144458194 1215 68-69 -0.026681048703935062 1215 70-71 0.05982510748943071 1215 72-73 0.0211592782465857 1215 74-75 0.0018910660882553998 1215 76-77 0.032389488796155774 1215 78-79 0.013506420586750778 1215 80-81 0.007866502390790231 1215 82-83 0.17179933659319602 1215 84-85 0.08188791509295612 1215 86-87 0.10628428552240621 1215 88-89 0.05017786063352503 1215 90-91 0.0421394919643987 1215 92-93 0.15311181102045168 1215 94-95 0.12762099718171527 1215 96-97 0.005328834084259881 1215 98-99 0.04554612041050632 1215 100-101 0.04511060720081872 1212 1 0.11158947463093938 1212 2 -0.02801633993792052 1212 3 -0.04551901505616485 1212 4 -0.08847314002001383 1212 5 0.042626896030093064 1212 6 -0.24959218352911705 1212 7 -0.30737437602626727 1212 8 -0.40050514567455053 1212 9 -0.4583025820084359 1212 10-11 -0.41081613708932707 1212 12-13 -0.4324111129454167 1212 14-15 -0.41682020029590916 1212 16-17 -0.3618934890761878 1212 18-19 -0.3270157947278278 1212 20-21 -0.3427495958725615 1212 22-23 -0.34629466667355047 1212 24-25 -0.2727264377781182 1212 26-27 -0.24377069209523938 1212 28-29 -0.25906538896215636 1212 30-31 -0.21925469963248645 1212 32-33 -0.23465578884096772 1212 34-35 -0.21531230713226535 1212 36-37 -0.18539701662905372 1212 38-39 -0.1953479384901371 1212 40-41 -0.21233124504226453 1212 42-43 -0.02753325081775415 1212 44-45 -0.024600293961974273 1212 46-47 -0.0017371707912658962 1212 48-49 0.048023921470949915 1212 50-51 -0.15605435287215386 1212 52-53 -0.07381348966611512 1212 54-55 -0.07545169977586852 1212 56-57 0.002762584991494066 1212 58-59 0.059494353047952586 1212 60-61 -0.009179894121423615 1212 62-63 0.045722281735777415 1212 64-65 -0.07423117825210568 1212 66-67 0.01405180622863611 1212 68-69 0.040309773535604165 1212 70-71 -0.08075388409538675 1212 72-73 -0.007926621194549455 1212 74-75 0.03767377326915522 1212 76-77 -8.827358015111031E-4 1212 78-79 0.10107043574409147 1212 80-81 -0.06936140108929578 1212 82-83 -0.041821879428987074 1212 84-85 -0.09976557848532508 1212 86-87 -0.11929385384647162 1212 88-89 -0.1498715024618278 1212 90-91 -0.10203082265540075 1212 92-93 -0.028104824561864916 1212 94-95 -0.1198511798488866 1212 96-97 -0.0560043260796661 1212 98-99 -0.020460376059432406 1212 100-101 -0.21663339138329718 1213 1 0.06727802266720317 1213 2 0.09517199070803173 1213 3 -0.040193724226206484 1213 4 -0.007472441881162695 1213 5 0.20001258393335775 1213 6 0.051439710138453165 1213 7 -0.060221628351854406 1213 8 -0.3873854647753987 1213 9 -0.3571746175639703 1213 10-11 -0.3737690262188309 1213 12-13 -0.2589728011935932 1213 14-15 -0.23662374010105935 1213 16-17 -0.2379866694708852 1213 18-19 -0.19120554170140736 1213 20-21 -0.2889004546641729 1213 22-23 -0.32348168794610643 1213 24-25 -0.4287603713474297 1213 26-27 -0.3160892937278774 1213 28-29 -0.35231326585659417 1213 30-31 -0.5628799570559693 1213 32-33 -0.39873879582319205 1213 34-35 -0.5422259478029545 1213 36-37 -0.5488849676714409 1213 38-39 -0.7066791568490558 1213 40-41 -0.4799196800502017 1213 42-43 -0.21772321211427936 1213 44-45 -0.2869235498028573 1213 46-47 -0.3257412204665542 1213 48-49 -0.22899370647952821 1213 50-51 -0.49534654970602077 1213 52-53 -0.46005081831302874 1213 54-55 -0.2396405303471525 1213 56-57 -0.2715822918239965 1213 58-59 -0.3482245500965888 1213 60-61 -0.30801227447815194 1213 62-63 -0.4601067164564938 1213 64-65 -0.5008244471800438 1213 66-67 -0.3401942170189258 1213 68-69 -0.30462942710359897 1213 70-71 -0.28828392913084855 1213 72-73 -0.2680579689188818 1213 74-75 -0.34217833573564604 1213 76-77 -0.38556200683862585 1213 78-79 -0.34767663005812466 1213 80-81 -0.30999608278848 1213 82-83 -0.16734156554678492 1213 84-85 -0.15209220276747715 1213 86-87 -0.2157826436788497 1213 88-89 -0.26278822204308483 1213 90-91 -0.3080702374197486 1213 92-93 -0.33710542587354553 1213 94-95 -0.4950204137072163 1213 96-97 -0.44818329543086577 1213 98-99 -0.3453909581285757 1213 100-101 -1.039115073462952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37.0 3 44.0 4 75.0 5 98.0 6 92.0 7 71.0 8 51.0 9 54.0 10 62.0 11 45.0 12 68.0 13 94.0 14 365.0 15 2902.0 16 13922.0 17 30066.0 18 38060.0 19 39633.0 20 42866.0 21 48588.0 22 55262.0 23 62476.0 24 70373.0 25 78976.0 26 88405.0 27 99863.0 28 113548.0 29 128455.0 30 148457.0 31 172877.0 32 204170.0 33 245175.0 34 306104.0 35 403063.0 36 581389.0 37 994384.0 38 2393789.0 39 3316612.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.868591837736073 30.90046252656752 29.19507981230357 20.035865823392843 2 21.482800279116805 31.21859484005791 28.506058807700196 18.79254607312509 3 21.713514911149193 29.43413425070201 28.88452086015503 19.96782997799377 4 21.323941857834534 28.97065310250289 29.045143749076747 20.660261290585833 5 21.5993881637412 29.05991850585668 29.364090715903384 19.976602614498734 6 21.86708937264199 29.171258115060027 27.90082595447198 21.060826557826008 7 21.952154881709625 29.597833795077953 28.668906789298987 19.781104533913435 8 21.037102481894436 29.440498812866867 29.2535803567536 20.268818348485095 9 21.18519150576702 29.0039295233241 29.24871509783417 20.562163873074713 10-11 21.281523996930982 29.286096739792605 28.78076960386332 20.651609659413094 12-13 21.13968633118483 29.341181019904816 29.09811163040229 20.42102101850806 14-15 21.30517942605549 29.163835071282318 28.950375878625557 20.58060962403664 16-17 21.40103034591837 29.104035865034593 28.921360200426143 20.573573588620892 18-19 21.180653766084593 28.942256522655786 29.060659351718293 20.816430359541332 20-21 21.417492390481225 28.863489358193707 28.780096027087566 20.9389222242375 22-23 21.409805781121385 28.773400079647534 28.755906141560576 21.060887997670505 24-25 21.726325830618475 28.692473900671047 28.63525177451362 20.945948494196855 26-27 21.832332995005256 28.741690775577204 28.470563365067154 20.955412864350382 28-29 21.662005947493054 28.86549074567477 28.434194685488514 21.038308621343667 30-31 21.863027582053622 28.481706755042545 28.5017740206823 21.15349164222153 32-33 21.891836801652577 28.726322394937064 28.38183021128196 21.000010592128397 34-35 22.045867797498712 28.51658986113219 28.26871236674318 21.168829974625915 36-37 21.959842037335438 28.698028328897003 28.241057304373136 21.101072329394427 38-39 21.91965052380717 28.50831873118245 28.416257499898222 21.155773245112165 40-41 22.06362159876269 28.658435678191136 28.222634004145203 21.05530871890097 42-43 21.976245201185794 28.65763307981991 28.238555972287983 21.127565746706313 44-45 21.966961638445156 28.370602747084956 28.291970241781524 21.37046537268837 46-47 22.056838326804595 28.64310768911722 28.087488615114914 21.212565368963272 48-49 21.92889107611786 28.40789735457974 28.271185785716458 21.392025783585943 50-51 21.996824966253964 28.5423339985662 28.07631448076314 21.384526554416695 52-53 21.99674553058102 28.580117853620795 28.19393376654617 21.22920284925202 54-55 22.0719452813447 28.372954304574378 28.152516895499772 21.40258351858115 56-57 22.17013440609402 28.5621551595249 27.987973392178944 21.279737042202136 58-59 22.027494199799673 28.517100542523053 28.119135608446026 21.336269649231244 60-61 22.11001882598342 28.401902531554075 27.870985722126584 21.617092920335924 62-63 22.027350472745237 28.494802290074716 28.107405543820928 21.370441693359112 64-65 22.12862645771544 28.307193764156857 28.01427896978723 21.549900808340468 66-67 22.07272735163043 28.494886469486357 27.8579773586824 21.57440882020081 68-69 21.922753150172852 28.378162507577787 28.070096583360897 21.628987758888467 70-71 22.238166723257446 28.38801731739864 27.791830178888173 21.581985780455742 72-73 22.062241280497872 28.549912131731926 27.90187461261182 21.485971975158378 74-75 22.174537622302008 28.378152861726992 27.887668667394543 21.559640848576457 76-77 22.129379027520063 28.489471981627833 27.856802478514425 21.524346512337676 78-79 22.195819737474565 28.42308085582698 27.948828161107237 21.43227124559121 80-81 22.224547430502753 28.385217772548643 27.77785270115571 21.6123820957929 82-83 22.159138383015645 28.730433831838393 27.886490840645784 21.22393694450018 84-85 22.198123832627495 28.374826274817927 27.844608880311974 21.582441012242604 86-87 22.058830672811936 28.592541031766576 27.78136234611634 21.567265949305142 88-89 22.139848223113223 28.440430786238007 27.82570917650094 21.59401181414783 90-91 22.165615588869443 28.37801014712919 27.766540684092767 21.689833579908605 92-93 22.01055324106396 28.473540053853487 27.918535775526482 21.597370929556075 94-95 22.144498269323986 28.33063405065005 27.785029264909706 21.739838415116257 96-97 22.13468238483791 28.52204899031466 27.631656600010274 21.71161202483716 98-99 22.052136652490255 28.508754522374048 27.793965165472066 21.645143659663628 100-101 22.414086076664255 28.202298383433316 27.325662821694973 22.057952718207456 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 135.0 1 128.5 2 162.5 3 195.5 4 260.0 5 320.0 6 327.0 7 427.5 8 516.0 9 552.5 10 620.0 11 797.5 12 993.5 13 1177.0 14 1548.5 15 2119.5 16 2863.0 17 3838.0 18 5207.0 19 7347.0 20 9956.5 21 13090.5 22 17731.0 23 22918.5 24 28432.5 25 35632.5 26 43740.5 27 52775.0 28 63881.0 29 78282.0 30 95123.5 31 116079.0 32 146874.5 33 190132.0 34 240335.0 35 303101.0 36 383049.0 37 459075.0 38 518007.5 39 551034.0 40 557054.5 41 537809.5 42 496545.5 43 444485.0 44 392458.5 45 349126.0 46 315185.0 47 295286.0 48 284320.0 49 272501.0 50 260675.0 51 244856.0 52 224350.5 53 205264.0 54 186979.5 55 171229.5 56 158977.5 57 145608.5 58 131036.0 59 115580.5 60 97744.5 61 80310.5 62 64858.5 63 52052.0 64 41842.5 65 33051.0 66 25951.0 67 20136.0 68 15335.0 69 11993.5 70 9283.5 71 7281.5 72 5966.5 73 4851.0 74 4044.0 75 3407.0 76 2814.5 77 2325.0 78 1961.0 79 1690.5 80 1458.5 81 1156.5 82 864.5 83 685.0 84 510.0 85 329.5 86 194.0 87 117.5 88 76.0 89 44.0 90 32.5 91 25.5 92 18.5 93 13.5 94 11.0 95 10.0 96 9.5 97 10.0 98 9.5 99 9.5 100 22.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.7517858192455795E-4 2 3.7187889020182796E-4 3 0.0013428959923954898 4 0.001663125036735953 5 0.00885278358063796 6 0.013893808536707183 7 0.013831828721673546 8 0.009803140744487076 9 0.002706451923135526 10-11 0.006239301380052891 12-13 0.010645033232027326 14-15 0.03420769291398204 16-17 0.014405142010734695 18-19 0.022421198088418543 20-21 0.017968981375168884 22-23 0.03029779958227671 24-25 0.025788768038579542 26-27 0.02164645040049807 28-29 0.029750311216146238 30-31 0.03221917384831948 32-33 0.03691414483711756 34-35 0.018573284571746854 36-37 0.028040701318135058 38-39 0.028645004514713027 40-41 0.03221400886373335 42-43 0.039579276883563996 44-45 0.027265953630214584 46-47 0.02807169122565187 48-49 0.02236954824255718 50-51 0.03624269684091982 52-53 0.028954903589881217 54-55 0.030927927701785363 56-57 0.02442004712325337 58-59 0.025380734256274757 60-61 0.033618884671162474 62-63 0.02991042573831647 64-65 0.029357772387599863 66-67 0.025039845273589746 68-69 0.026961219539632528 70-71 0.03000856044545306 72-73 0.037032939482598705 74-75 0.03195059464984039 76-77 0.03432132257487704 78-79 0.03565388859810026 80-81 0.04151098111877905 82-83 0.04345301532316637 84-85 0.04884525923109287 86-87 0.04995573091711223 88-89 0.05219733422749547 90-91 0.05192359004443023 92-93 0.05226964401170138 94-95 0.05331297089810094 96-97 0.05402573877098778 98-99 0.055193025287454636 100-101 0.058715544775199725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9680571.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.769727117987026 #Duplication Level Percentage of deduplicated Percentage of total 1 40.08258767141376 6.32091469760986 2 2.087682289444286 0.6584436002718159 3 3.599137052857041 1.7027222755137474 4 4.868739610530249 3.071147802663858 5 5.221773920799038 4.1172974901411 6 5.615871530014758 5.3136456934803 7 4.939659908595354 5.452796216994662 8 4.718884007895655 5.9532410484757925 9 3.7924775545453158 5.382570252264334 >10 24.70387057278126 57.30032485028178 >50 0.3032900187535545 3.2328632855016295 >100 0.06534570997187356 1.3821424482369258 >500 3.6202842568943403E-4 0.05368828238523574 >1k 3.181239721749338E-4 0.058202056179024 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.164984586136499E-5 1.2395963006727598E-4 0.0 2 5.164984586136499E-5 1.2395963006727598E-4 0.0 3 5.164984586136499E-5 1.2395963006727598E-4 0.0 4 5.164984586136499E-5 1.2395963006727598E-4 0.0 5 5.164984586136499E-5 1.2395963006727598E-4 0.0 6 5.164984586136499E-5 1.2395963006727598E-4 0.0 7 5.164984586136499E-5 1.2395963006727598E-4 0.0 8 6.197981503363799E-5 1.2395963006727598E-4 0.0 9 6.197981503363799E-5 1.2395963006727598E-4 0.0 10-11 6.71447996197745E-5 1.2395963006727598E-4 0.0 12-13 9.81347071365935E-5 1.2912461465341247E-4 0.0 14-15 2.0143439885932345E-4 1.34289599239549E-4 0.0 16-17 3.2022904434046294E-4 2.58249229306825E-4 0.0 18-19 3.8737384396023746E-4 2.89239136823644E-4 1.0329969172272999E-4 20-21 5.474883661304689E-4 2.89239136823644E-4 1.0329969172272999E-4 22-23 7.85077657092748E-4 2.89239136823644E-4 1.0329969172272999E-4 24-25 9.658521176075254E-4 2.89239136823644E-4 1.0329969172272999E-4 26-27 0.0012964111311202613 2.99569105995917E-4 1.0329969172272999E-4 28-29 0.0014926805453934484 3.0989907516818996E-4 1.0329969172272999E-4 30-31 0.0017509297747002734 3.0989907516818996E-4 2.27259321790006E-4 32-33 0.002086653772799146 3.0989907516818996E-4 2.27259321790006E-4 34-35 0.0024430377092425643 3.0989907516818996E-4 2.27259321790006E-4 36-37 0.002861401460719621 3.0989907516818996E-4 2.3758929096227897E-4 38-39 0.003264270258438268 3.0989907516818996E-4 2.3758929096227897E-4 40-41 0.0036826340099153244 3.0989907516818996E-4 2.3758929096227897E-4 42-43 0.004142317638081473 3.150640597543265E-4 2.3758929096227897E-4 44-45 0.004694970988798078 3.20229044340463E-4 2.3758929096227897E-4 46-47 0.005180479539894909 3.20229044340463E-4 2.3758929096227897E-4 48-49 0.005717637936853105 3.20229044340463E-4 2.3758929096227897E-4 50-51 0.006456230732670624 3.460539672711455E-4 2.3758929096227897E-4 52-53 0.006900419407078364 3.7187889020182796E-4 2.3758929096227897E-4 54-55 0.007520217557414744 3.7187889020182796E-4 2.3758929096227897E-4 56-57 0.008186500569026353 3.7187889020182796E-4 4.0286879771864696E-4 58-59 0.00866167915095091 3.7187889020182796E-4 4.0286879771864696E-4 60-61 0.009338292131734791 3.7187889020182796E-4 4.0286879771864696E-4 62-63 0.010113039819655266 3.7187889020182796E-4 4.0286879771864696E-4 64-65 0.010980757230126198 3.7187889020182796E-4 4.0286879771864696E-4 66-67 0.011982764239836678 3.7187889020182796E-4 4.0286879771864696E-4 68-69 0.013134555802545118 3.7187889020182796E-4 4.0286879771864696E-4 70-71 0.014162387735186282 3.7187889020182796E-4 4.0286879771864696E-4 72-73 0.01505076508400176 3.7187889020182796E-4 4.0286879771864696E-4 74-75 0.01601145221702315 3.7187889020182796E-4 4.0286879771864696E-4 76-77 0.01744215294738296 3.7187889020182796E-4 4.080337823047835E-4 78-79 0.018573284571746854 4.23528736063193E-4 4.1319876689091995E-4 80-81 0.019626941427318696 6.09468181164107E-4 4.1836375147705646E-4 82-83 0.020861372743405323 6.09468181164107E-4 4.23528736063193E-4 84-85 0.022230093658731492 6.09468181164107E-4 4.23528736063193E-4 86-87 0.023516174820679484 6.09468181164107E-4 4.23528736063193E-4 88-89 0.024838410874730425 6.09468181164107E-4 4.2869372064932945E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGATCG 870 0.0 40.39234 1 TCGTGAC 740 0.0 30.803375 5 TCGCGAC 100 0.0016568559 23.744267 5 CGATCTA 525 0.0 18.09648 34-35 TGATCGT 1600 0.0 17.511307 2 CGTGACT 1220 0.0 17.12719 6 CGCGACT 200 2.7678628E-4 16.621159 6 ATCGTGA 1945 0.0 15.137655 4 GATCGTG 2240 0.0 15.052092 3 TCCGATC 820 0.0 13.61409 32-33 TGATCGG 635 1.3460522E-10 12.713401 2 CCGATCT 860 0.0 12.704688 32-33 ATCGGGA 565 5.576112E-8 11.76701 4 GATCGGG 570 7.9180063E-7 10.830664 3 CTTCCGA 1125 0.0 10.555843 30-31 GACCGGA 595 1.3709778E-6 10.378113 9 TTCCGAT 1075 0.0 9.500259 30-31 GTGACTG 2890 0.0 9.366106 7 TGATCGA 915 5.5952114E-9 9.341959 2 GTAACGT 435 0.009007946 8.734968 8 >>END_MODULE