FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 10-60155.34200000001bc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 10-60155.34200000001bc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9680571
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCG8700.040.392341
TCGTGAC7400.030.8033755
TCGCGAC1000.001656855923.7442675
CGATCTA5250.018.0964834-35
TGATCGT16000.017.5113072
CGTGACT12200.017.127196
CGCGACT2002.7678628E-416.6211596
ATCGTGA19450.015.1376554
GATCGTG22400.015.0520923
TCCGATC8200.013.6140932-33
TGATCGG6351.3460522E-1012.7134012
CCGATCT8600.012.70468832-33
ATCGGGA5655.576112E-811.767014
GATCGGG5707.9180063E-710.8306643
CTTCCGA11250.010.55584330-31
GACCGGA5951.3709778E-610.3781139
TTCCGAT10750.09.50025930-31
GTGACTG28900.09.3661067
TGATCGA9155.5952114E-99.3419592
GTAACGT4350.0090079468.7349688
Produced by FastQC (version 0.11.2)