##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n02 1-58812_2015-03-25 191131308797.34200000004434.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11904405 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.664980988130026 38.0 32.0 38.0 32.0 38.0 2 34.759381086244964 38.0 32.0 38.0 32.0 38.0 3 34.85215607163903 38.0 32.0 38.0 32.0 38.0 4 35.23104229064787 38.0 32.0 38.0 32.0 38.0 5 34.82918037482764 38.0 32.0 38.0 32.0 38.0 6 37.15847856318732 40.0 38.0 40.0 32.0 40.0 7 37.29280749436868 40.0 38.0 40.0 32.0 40.0 8 37.393981723572075 40.0 38.0 40.0 32.0 40.0 9 37.41188224022956 40.0 38.0 40.0 32.0 40.0 10-11 37.43745945303441 40.0 38.0 40.0 32.0 40.0 12-13 37.40476554687109 40.0 38.0 40.0 32.0 40.0 14-15 37.36527676099729 40.0 38.0 40.0 32.0 40.0 16-17 37.345671077218896 40.0 38.0 40.0 32.0 40.0 18-19 37.33513027320559 40.0 38.0 40.0 32.0 40.0 20-21 37.32324820098107 40.0 38.0 40.0 32.0 40.0 22-23 37.311081570225475 40.0 38.0 40.0 32.0 40.0 24-25 37.24580073510604 40.0 38.0 40.0 32.0 40.0 26-27 37.2304177319236 40.0 38.0 40.0 32.0 40.0 28-29 37.15940271689345 40.0 38.0 40.0 32.0 40.0 30-31 37.12204746898312 40.0 38.0 40.0 32.0 40.0 32-33 37.125115912975076 40.0 38.0 40.0 32.0 40.0 34-35 37.04029012789803 40.0 38.0 40.0 32.0 40.0 36-37 37.04168650176132 40.0 38.0 40.0 32.0 40.0 38-39 37.00132035158414 40.0 38.0 40.0 32.0 40.0 40-41 36.9003038791103 40.0 38.0 40.0 32.0 40.0 42-43 36.66989408542469 38.0 38.0 40.0 32.0 40.0 44-45 36.567403536758036 38.0 38.0 40.0 32.0 40.0 46-47 36.47718563002519 38.0 38.0 40.0 27.0 40.0 48-49 36.39435935689352 38.0 38.0 40.0 27.0 40.0 50-51 36.324024300248524 38.0 38.0 40.0 27.0 40.0 52-53 36.249699249983514 38.0 38.0 40.0 27.0 40.0 54-55 36.19574871654652 38.0 38.0 40.0 27.0 40.0 56-57 36.166695437529214 38.0 38.0 40.0 27.0 40.0 58-59 36.09478016750943 38.0 38.0 40.0 27.0 40.0 60-61 36.05579359069185 38.0 38.0 40.0 27.0 40.0 62-63 35.977445953829694 38.0 38.0 40.0 27.0 40.0 64-65 35.95490707011396 38.0 38.0 40.0 27.0 40.0 66-67 35.883694859171875 38.0 38.0 40.0 27.0 40.0 68-69 35.82638246094618 38.0 38.0 40.0 27.0 40.0 70-71 35.710151326336764 38.0 38.0 40.0 27.0 40.0 72-73 35.637573780461935 38.0 38.0 40.0 27.0 40.0 74-75 35.54468807974863 38.0 38.0 40.0 27.0 40.0 76-77 35.455115270355805 38.0 38.0 40.0 27.0 40.0 78-79 35.36363929990621 38.0 38.0 40.0 27.0 40.0 80-81 35.27981087673008 38.0 38.0 40.0 27.0 40.0 82-83 35.183814310753036 38.0 38.0 40.0 27.0 40.0 84-85 35.0761577752101 38.0 38.0 40.0 27.0 40.0 86-87 35.00707255003505 38.0 38.0 40.0 27.0 40.0 88-89 34.929304656553605 38.0 38.0 40.0 27.0 40.0 90-91 34.8041826953972 38.0 32.0 40.0 13.0 40.0 92-93 34.69524570946636 38.0 32.0 40.0 13.0 40.0 94-95 34.58612282596232 38.0 32.0 40.0 13.0 40.0 96-97 34.50027914036863 38.0 32.0 39.0 13.0 40.0 98-99 34.355637178002596 38.0 32.0 38.0 13.0 40.0 100-101 32.44492101873214 38.0 29.5 38.0 13.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.06475216189259214 2201 2 0.36579878574694646 2201 3 0.3816186091952929 2201 4 0.26674146812150923 2201 5 0.44986789294983254 2201 6 0.41540170028197565 2201 7 0.42511440282955704 2201 8 0.28693986559063944 2201 9 0.29295513829806197 2201 10-11 0.2858770396106891 2201 12-13 0.3277347989180228 2201 14-15 0.3025168953823112 2201 16-17 0.24559337247676893 2201 18-19 0.2605452257568217 2201 20-21 0.32836695649132963 2201 22-23 0.3000220122332138 2201 24-25 0.29079944281667736 2201 26-27 0.29606038616684316 2201 28-29 0.2916681412150979 2201 30-31 0.2899209487219778 2201 32-33 0.23778154637383153 2201 34-35 0.28988897546579295 2201 36-37 0.14837780436221237 2201 38-39 0.23730164116155805 2201 40-41 0.23986677902989584 2201 42-43 0.3179989619324033 2201 44-45 0.26984988707499724 2201 46-47 0.22696761682463773 2201 48-49 0.14267365560547773 2201 50-51 0.26728362047239074 2201 52-53 0.23539691115234973 2201 54-55 0.2992797263002771 2201 56-57 0.15505079620660922 2201 58-59 0.2045378954935373 2201 60-61 0.12857883160756955 2201 62-63 0.18604992658883646 2201 64-65 0.18298171027788612 2201 66-67 0.1268861690124794 2201 68-69 0.056928659095660805 2201 70-71 0.1392310660334175 2201 72-73 -0.011897964666069072 2201 74-75 0.11539918258940673 2201 76-77 0.09346366434179032 2201 78-79 0.09611490954645774 2201 80-81 0.23596712760005545 2201 82-83 0.13931286421436795 2201 84-85 0.14023302839164842 2201 86-87 0.15245222123515134 2201 88-89 0.13049206900675614 2201 90-91 0.05578652050201782 2201 92-93 -0.00733158699275549 2201 94-95 0.09869039976413063 2201 96-97 -0.1174302134557621 2201 98-99 -0.08098523913733402 2201 100-101 0.13194245003312943 1101 1 0.16399626335358164 1101 2 0.11478602527305526 1101 3 0.19872245280849654 1101 4 0.12546862109091705 1101 5 0.33961781212278197 1101 6 0.3831684039299219 1101 7 0.3612436013467004 1101 8 0.3561031149392946 1101 9 0.4061033801057832 1101 10-11 0.4027624410752324 1101 12-13 0.3614202944698164 1101 14-15 0.34315810259477786 1101 16-17 0.3104908259593486 1101 18-19 0.27924149556168487 1101 20-21 0.33415126653289207 1101 22-23 0.3247883939617111 1101 24-25 0.2701944849442768 1101 26-27 0.28646918747365646 1101 28-29 0.36509094102966344 1101 30-31 0.31617553547953037 1101 32-33 0.24565951208489878 1101 34-35 0.2467235371383154 1101 36-37 0.28822598383246145 1101 38-39 0.29589454276985094 1101 40-41 0.3155393595411269 1101 42-43 0.2826051099984923 1101 44-45 0.2326912566566932 1101 46-47 0.2626650989634385 1101 48-49 0.19282664915306924 1101 50-51 0.22120235446362102 1101 52-53 0.29339021244389585 1101 54-55 0.20475494450457887 1101 56-57 0.25393728565577334 1101 58-59 0.19439628749000093 1101 60-61 0.13832249787790118 1101 62-63 0.22560892367957308 1101 64-65 0.15151889078516945 1101 66-67 0.21461575790344511 1101 68-69 0.1788533322748691 1101 70-71 0.20671847299212232 1101 72-73 0.24388801148064232 1101 74-75 0.23201322041963124 1101 76-77 0.18200658590821206 1101 78-79 0.11229345303006966 1101 80-81 0.214087359845621 1101 82-83 0.23062533741103408 1101 84-85 0.1622975243078031 1101 86-87 0.23013929846545977 1101 88-89 0.13289939141878193 1101 90-91 0.08768223002943643 1101 92-93 0.09697865936158223 1101 94-95 0.09661304997641906 1101 96-97 0.08545656293861015 1101 98-99 0.0656550883556406 1101 100-101 0.1922685280019678 2202 1 -0.07975559783968578 2202 2 0.0019040908224141617 2202 3 0.07524937220680528 2202 4 0.10050373198176032 2202 5 0.08815803940474609 2202 6 0.17737765661163962 2202 7 0.2635763287188695 2202 8 0.14309169708361225 2202 9 0.25484453702905085 2202 10-11 0.2053702457669644 2202 12-13 0.19972372573524666 2202 14-15 0.20206991520352346 2202 16-17 0.18390457200960952 2202 18-19 0.1193082131882619 2202 20-21 0.17157516972580567 2202 22-23 0.19677394262794223 2202 24-25 0.16733987730190591 2202 26-27 0.16597595567787948 2202 28-29 0.1795032869889468 2202 30-31 0.18836677428337367 2202 32-33 0.2295793317499104 2202 34-35 0.18919290037502634 2202 36-37 0.23571403397617985 2202 38-39 0.19907091799070287 2202 40-41 0.14108228857931238 2202 42-43 0.1562191076141346 2202 44-45 0.10087461491507099 2202 46-47 0.029234185412128966 2202 48-49 0.04577810857317388 2202 50-51 0.11791594862526011 2202 52-53 0.13684789730878322 2202 54-55 0.08550394350762502 2202 56-57 0.0957573047041933 2202 58-59 0.11656034759162281 2202 60-61 0.01077487348988626 2202 62-63 0.042709997725040694 2202 64-65 0.008104555840716898 2202 66-67 0.04199785341069884 2202 68-69 0.06787648079723141 2202 70-71 0.028472629611997036 2202 72-73 -0.004960123554781148 2202 74-75 0.026534981530019763 2202 76-77 -0.05559913007308381 2202 78-79 -0.07256869470003835 2202 80-81 -6.499532173762645E-4 2202 82-83 -0.08947217657687645 2202 84-85 -0.02664545531531104 2202 86-87 -0.02928125999002873 2202 88-89 -0.05094063252148118 2202 90-91 -0.018789276408163857 2202 92-93 -0.08374634087475386 2202 94-95 5.54064401335097E-4 2202 96-97 -0.037258628184901 2202 98-99 -0.08729349234516803 2202 100-101 0.03231832670361001 1102 1 -0.21265383932615123 1102 2 -0.23862519105725966 1102 3 -0.17608116089055414 1102 4 0.019082665538093124 1102 5 -0.17939362868160913 1102 6 -0.09788640597316345 1102 7 0.09897727939024037 1102 8 0.18221161095714677 1102 9 0.16604018092269968 1102 10-11 0.0992318578775766 1102 12-13 0.1672589542151499 1102 14-15 0.15041681970673437 1102 16-17 0.15904613399501244 1102 18-19 0.1354768534771793 1102 20-21 0.19739104349464043 1102 22-23 0.17557264682589135 1102 24-25 0.16335727977071457 1102 26-27 0.1750593406688452 1102 28-29 0.024724762187680938 1102 30-31 0.1286085989150223 1102 32-33 0.07649690954503541 1102 34-35 0.15707324084652896 1102 36-37 0.1355568328374659 1102 38-39 0.13887742997398078 1102 40-41 0.16388650225916024 1102 42-43 0.01807189759485084 1102 44-45 -0.017818876488043145 1102 46-47 0.001279854775361855 1102 48-49 -0.0854348492093635 1102 50-51 -0.00696689882425261 1102 52-53 0.02270258134694103 1102 54-55 -0.036247200671290614 1102 56-57 -0.11167076914794194 1102 58-59 -0.09309559820471947 1102 60-61 -0.06408111778470271 1102 62-63 -0.13725615763702592 1102 64-65 -0.23203047903859897 1102 66-67 -0.253233890011451 1102 68-69 -0.260681482259983 1102 70-71 -0.1355370551157904 1102 72-73 -0.12294324045382865 1102 74-75 -0.0740684388599675 1102 76-77 -0.18431231925865887 1102 78-79 -0.19185734722022119 1102 80-81 -0.10248951310742171 1102 82-83 -0.15829696517410952 1102 84-85 -0.13794441677180203 1102 86-87 -0.16856994324699315 1102 88-89 -0.2807233010691519 1102 90-91 -0.3667160505593259 1102 92-93 -0.25448154414591784 1102 94-95 -0.2200520191827806 1102 96-97 -0.27601429431764757 1102 98-99 -0.32240331756526075 1102 100-101 -0.33997862240074994 2203 1 -0.298910121756748 2203 2 -0.29061979930582993 2203 3 -0.28011694451699753 2203 4 -0.10999892287167512 2203 5 -0.2411655249999356 2203 6 -0.08715966215589077 2203 7 0.0714783584373535 2203 8 0.04655225733242219 2203 9 0.11813219375845563 2203 10-11 0.056571535775681525 2203 12-13 0.10963269006835219 2203 14-15 0.10015537226307458 2203 16-17 0.07151455619554525 2203 18-19 0.08602077913725026 2203 20-21 0.1379492941119551 2203 22-23 0.07061074685247348 2203 24-25 0.06775239859454274 2203 26-27 0.026811399315647577 2203 28-29 0.0076789333725884035 2203 30-31 0.02721589631100585 2203 32-33 0.016262506473545102 2203 34-35 0.006816584866406572 2203 36-37 -0.02279853929248077 2203 38-39 -0.024131867013792885 2203 40-41 0.023103562078958362 2203 42-43 -0.08862125359615902 2203 44-45 -0.181155896627736 2203 46-47 -0.23468668481775978 2203 48-49 -0.2571245643343758 2203 50-51 -0.14197532885193453 2203 52-53 -0.1482083460010699 2203 54-55 -0.11689243809053806 2203 56-57 -0.25362261127656893 2203 58-59 -0.18449345751341895 2203 60-61 -0.2582236526279402 2203 62-63 -0.31469813789738765 2203 64-65 -0.30863090528528403 2203 66-67 -0.3089071041877247 2203 68-69 -0.18837938816707123 2203 70-71 -0.1749589404724219 2203 72-73 -0.21455515593920182 2203 74-75 -0.20309372236184942 2203 76-77 -0.21030846944129422 2203 78-79 -0.22216996133015954 2203 80-81 -0.16703588345617248 2203 82-83 -0.21697521464552239 2203 84-85 -0.2944241014130995 2203 86-87 -0.457638893687772 2203 88-89 -0.47671381018940195 2203 90-91 -0.2733065843247928 2203 92-93 -0.38786540672305136 2203 94-95 -0.268813044685821 2203 96-97 -0.2669617337196257 2203 98-99 -0.3161105387147387 2203 100-101 -0.16534133455255073 1103 1 -0.27069081404790296 1103 2 -0.19654293328044048 1103 3 -0.19276314592413257 1103 4 0.004785417698037975 1103 5 -0.11648241502648204 1103 6 -0.060223982221558 1103 7 0.033536125687163576 1103 8 0.07520655993008063 1103 9 0.11696875742883606 1103 10-11 0.15615775492332773 1103 12-13 0.12733841238325283 1103 14-15 0.15349478659639004 1103 16-17 0.10535229788779787 1103 18-19 0.10976748225635191 1103 20-21 0.13827310994997077 1103 22-23 0.1467236918110828 1103 24-25 0.12705305002617706 1103 26-27 0.10271417174378428 1103 28-29 0.07197460289846447 1103 30-31 0.10213183747727328 1103 32-33 0.07940642346108717 1103 34-35 0.07219853091425676 1103 36-37 0.07239695466046214 1103 38-39 0.10514490326986703 1103 40-41 0.012670741807454533 1103 42-43 -0.08209778738974194 1103 44-45 -0.14514177872219136 1103 46-47 -0.2121847117159419 1103 48-49 -0.060251268667023794 1103 50-51 -0.109148850277343 1103 52-53 -0.1049673772967381 1103 54-55 -0.03871153885896206 1103 56-57 -0.08658148650978603 1103 58-59 -0.12273934205284576 1103 60-61 -0.17589004683352272 1103 62-63 -0.1488409206482686 1103 64-65 -0.08284052082230176 1103 66-67 -0.21603791325786403 1103 68-69 -0.20943758180472827 1103 70-71 -0.11336849987438313 1103 72-73 -0.10518266249456332 1103 74-75 -0.11478982600404919 1103 76-77 -0.2529653092948507 1103 78-79 -0.15989168238564844 1103 80-81 -0.14690131289452069 1103 82-83 -0.306325916560489 1103 84-85 -0.13401382194817302 1103 86-87 -0.34347577617583624 1103 88-89 -0.33613578162408686 1103 90-91 -0.22871981179080336 1103 92-93 -0.17600250861210043 1103 94-95 -0.15418490585216205 1103 96-97 -0.24771899196470315 1103 98-99 -0.20280601290005507 1103 100-101 -0.08913084074013966 2204 1 -0.15183681303197716 2204 2 -0.16843355762900103 2204 3 0.0011928427731149327 2204 4 0.016446090094362376 2204 5 0.002451787539868633 2204 6 0.07734752572244474 2204 7 0.10811066862936514 2204 8 0.15532724514027763 2204 9 0.1340912183724825 2204 10-11 0.17472025316843798 2204 12-13 0.16887107371166366 2204 14-15 0.14873424684829217 2204 16-17 0.13434040209025255 2204 18-19 0.19343743066904295 2204 20-21 0.18237975946340867 2204 22-23 0.1337458114769703 2204 24-25 0.1442275407259075 2204 26-27 0.14335726463413323 2204 28-29 0.1730492662365961 2204 30-31 0.09918164395040208 2204 32-33 0.1173574295540547 2204 34-35 0.11368040473421814 2204 36-37 0.16335333219824122 2204 38-39 0.18228149690769158 2204 40-41 0.08273032715199236 2204 42-43 0.07323319077818269 2204 44-45 0.04358033983758958 2204 46-47 0.10053626159360363 2204 48-49 0.0018084630244956656 2204 50-51 0.06407867078343088 2204 52-53 0.1114722388317233 2204 54-55 0.038366779750333535 2204 56-57 0.0674616552196099 2204 58-59 0.1384926868353702 2204 60-61 0.05352899818434764 2204 62-63 0.12286699119299271 2204 64-65 0.028072982154412784 2204 66-67 0.05514481711738739 2204 68-69 0.03842998098195238 2204 70-71 0.04025178415788844 2204 72-73 0.09655879003327783 2204 74-75 0.0513421636597613 2204 76-77 0.10413651842582539 2204 78-79 0.03628879747930114 2204 80-81 -0.04425309615803741 2204 82-83 -0.03141523501748367 2204 84-85 0.12436325807215098 2204 86-87 0.051399109916012264 2204 88-89 0.06236071583312963 2204 90-91 0.11427429433969394 2204 92-93 0.08419836963997085 2204 94-95 -0.015361203766971698 2204 96-97 -0.024000451248340937 2204 98-99 -0.04888275657756935 2204 100-101 0.11015964866289352 2205 1 -0.0742819121753513 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48-49 0.06448739416597249 1215 50-51 0.039269622371840285 1215 52-53 0.05802369279850694 1215 54-55 0.011420782746490943 1215 56-57 -0.030533726390949312 1215 58-59 0.04605657938726182 1215 60-61 0.021190284934149872 1215 62-63 -0.03148883263218494 1215 64-65 -0.08364977172711008 1215 66-67 -0.004598491441242913 1215 68-69 0.022944899681569098 1215 70-71 0.1159966197822655 1215 72-73 0.12951843163225618 1215 74-75 0.112285460404415 1215 76-77 0.17506147616077072 1215 78-79 0.14584600583681606 1215 80-81 0.14419873426984253 1215 82-83 0.2681912119128853 1215 84-85 0.19828479248883468 1215 86-87 0.24529324247210127 1215 88-89 0.2276592539083211 1215 90-91 0.14112175950350547 1215 92-93 0.19893322245478373 1215 94-95 0.16361159291286498 1215 96-97 0.19081339659094 1215 98-99 0.20880762482242687 1215 100-101 0.21480988934530387 1212 1 0.36338609026532254 1212 2 0.22500921092369452 1212 3 0.16340448126632623 1212 4 -0.12332594230747418 1212 5 0.17945289196411096 1212 6 -0.07966401700551984 1212 7 -0.264000779922263 1212 8 -0.33939173800305156 1212 9 -0.41879215297340266 1212 10-11 -0.3463351331282425 1212 12-13 -0.3890342824362065 1212 14-15 -0.36923600129301803 1212 16-17 -0.29495669287811666 1212 18-19 -0.25872189511780164 1212 20-21 -0.2582328580112687 1212 22-23 -0.24842652179361835 1212 24-25 -0.13071498800972137 1212 26-27 -0.1413767673330426 1212 28-29 -0.10756311155567744 1212 30-31 -0.1433415870654713 1212 32-33 -0.12637105120685277 1212 34-35 -0.09635965454543083 1212 36-37 -0.12316380226655355 1212 38-39 -0.057689671183204894 1212 40-41 -0.1407185923516039 1212 42-43 0.08935734842241772 1212 44-45 0.08876238940229086 1212 46-47 0.09779421130715349 1212 48-49 0.16852019537088125 1212 50-51 -0.06194480678215797 1212 52-53 0.07027477741857524 1212 54-55 0.09509540566721597 1212 56-57 0.20339607326398124 1212 58-59 0.13788118967399043 1212 60-61 0.12792775485203123 1212 62-63 0.18477330660912372 1212 64-65 0.11068927188682665 1212 66-67 0.195282332881888 1212 68-69 0.15822785330232847 1212 70-71 0.10217687559402577 1212 72-73 0.09414031908318776 1212 74-75 0.15914141590449304 1212 76-77 0.19098149534224973 1212 78-79 0.2411170434860992 1212 80-81 0.12485465239733884 1212 82-83 0.1348491960855469 1212 84-85 0.06147885528397268 1212 86-87 0.05858141424427288 1212 88-89 0.0052284270303673 1212 90-91 0.09941189762867708 1212 92-93 0.08523611638830175 1212 94-95 0.10171157377785534 1212 96-97 0.18143170287446253 1212 98-99 0.17437430658036135 1212 100-101 -0.026837125319552513 1213 1 0.14825444924372277 1213 2 0.21296687579584983 1213 3 0.07133984031649021 1213 4 -0.12485704850741541 1213 5 0.1119128705137129 1213 6 0.0026913564106720855 1213 7 -0.1430889852087205 1213 8 -0.41591146802293366 1213 9 -0.43560634665092124 1213 10-11 -0.497239380368498 1213 12-13 -0.38130973757565556 1213 14-15 -0.29887475119409146 1213 16-17 -0.26726227390441437 1213 18-19 -0.19515826558059501 1213 20-21 -0.3132900868046775 1213 22-23 -0.35228886199527665 1213 24-25 -0.45260655364243263 1213 26-27 -0.3297866781903309 1213 28-29 -0.36466088405330765 1213 30-31 -0.5875820208143878 1213 32-33 -0.4947902708404328 1213 34-35 -0.534113594867911 1213 36-37 -0.5161229276120665 1213 38-39 -0.7141665711935232 1213 40-41 -0.5178589350684888 1213 42-43 -0.16880558317494376 1213 44-45 -0.26577604567057733 1213 46-47 -0.31736324308214137 1213 48-49 -0.17584839030281074 1213 50-51 -0.4731650072333551 1213 52-53 -0.41313339434966423 1213 54-55 -0.18421780814902178 1213 56-57 -0.26212363424074425 1213 58-59 -0.3386684990633313 1213 60-61 -0.2859317080778183 1213 62-63 -0.44843972874998883 1213 64-65 -0.4607403941034107 1213 66-67 -0.2501345932926995 1213 68-69 -0.26557564853552407 1213 70-71 -0.26881285621276163 1213 72-73 -0.1694044888342532 1213 74-75 -0.25016141910057144 1213 76-77 -0.26110812614025036 1213 78-79 -0.2371454352402793 1213 80-81 -0.17876691489727392 1213 82-83 -0.11358370357869774 1213 84-85 -0.10286492832169358 1213 86-87 -0.07748931453382113 1213 88-89 -0.13239850218565863 1213 90-91 -0.17915824059708996 1213 92-93 -0.24711238576242067 1213 94-95 -0.25465674517777614 1213 96-97 -0.27634128346636544 1213 98-99 -0.21621046710198755 1213 100-101 -0.8064332470191928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57.0 3 56.0 4 72.0 5 124.0 6 124.0 7 74.0 8 62.0 9 67.0 10 49.0 11 79.0 12 82.0 13 128.0 14 589.0 15 4702.0 16 23069.0 17 50442.0 18 68694.0 19 75021.0 20 78391.0 21 83064.0 22 91059.0 23 99540.0 24 108899.0 25 118348.0 26 129682.0 27 142908.0 28 158871.0 29 177379.0 30 201657.0 31 230647.0 32 268177.0 33 319133.0 34 395906.0 35 518178.0 36 744009.0 37 1274395.0 38 2986245.0 39 3554426.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.96224094072724 32.20553845905714 31.09389178148911 18.73832881872651 2 19.56784923579286 32.978527874315674 29.43344978362557 18.020173106265904 3 19.73156634461068 31.861197413191682 29.842102544092462 18.56513369810517 4 19.940750303506427 30.439485371376612 30.33603445373379 19.283729871383166 5 20.178342906679887 30.53707268056476 30.238199110318504 19.04638530243685 6 20.599856874084985 30.812003488925217 29.115176707072266 19.472962929917532 7 20.43600095372373 31.02509002489999 29.830767806413572 18.70814121496271 8 19.68488008093233 31.20983984411689 30.14211715461909 18.963162920331694 9 19.772489768213923 30.676347941210075 30.431440312901124 19.11972197767488 10-11 19.70692670395346 30.982845427389982 30.041657294562963 19.268570574093594 12-13 19.528549292740767 31.047332752421802 30.332449476322704 19.09166847851473 14-15 19.678473381521723 30.881528762935805 30.24829196520416 19.191705890338305 16-17 19.792028542117073 30.815506342221266 30.261913041345018 19.13055207431664 18-19 19.62021921065227 30.65182487127305 30.341633324831847 19.386322593242834 20-21 19.862506804862527 30.59880815818107 30.086148225795533 19.45253681116087 22-23 19.83422064177867 30.585452158006653 30.063473885562313 19.51685331465236 24-25 19.998648896064903 30.420331963211993 30.032897868389874 19.548121272333233 26-27 20.129677837857457 30.47663186305995 29.8568053894345 19.536884909648094 28-29 20.02055296811202 30.540983782187276 29.865609552844802 19.572853696855905 30-31 20.174730318785777 30.286720235281763 29.85792762985137 19.680621816081086 32-33 20.173836368924324 30.44003667532514 29.822945170456144 19.563181785294393 34-35 20.258093098404753 30.299698237867606 29.72119023995074 19.721018423776908 36-37 20.21487626083074 30.446630818945707 29.67478083085219 19.66371208937137 38-39 20.146974593828666 30.32343342586396 29.845360151236385 19.684231829070992 40-41 20.30329321102675 30.40671574223186 29.643151093276487 19.646839953464905 42-43 20.24667453628722 30.386589857332524 29.758884395150865 19.607851211229395 44-45 20.246287427844038 30.24290416025958 29.711546714573256 19.799261697323125 46-47 20.327414453782612 30.402092502874407 29.581222561222948 19.689270482120033 48-49 20.205481049023774 30.167749448561576 29.76703367613083 19.85973582628382 50-51 20.302443543668772 30.367679636645907 29.512528636508307 19.817348183177018 52-53 20.27444647469869 30.413608611385083 29.650030980376407 19.661913933539825 54-55 20.388305379633092 30.164153545638523 29.61010439641674 19.837436678311644 56-57 20.38792438511484 30.397576891523236 29.489280911798875 19.725217811563045 58-59 20.23667028682611 30.363256553772054 29.654193480192408 19.745879679209423 60-61 20.338955634174326 30.267638258871543 29.437309429731574 19.956096677222558 62-63 20.232421829293617 30.345845130623527 29.592168600567277 19.829564439515575 64-65 20.339141349158417 30.210283853687997 29.5481427408555 19.902432056298085 66-67 20.35646597352111 30.328343756927996 29.38291230110765 19.932277968443245 68-69 20.189925308313008 30.235267155592595 29.650648261328687 19.924159274765714 70-71 20.441633759721412 30.23780263210467 29.36977687278222 19.95078673539169 72-73 20.362579181738873 30.29567498998851 29.451621592516652 19.890124235755962 74-75 20.35261340900313 30.23195322845086 29.46685238756006 19.94858097498595 76-77 20.367687996068366 30.363143186459833 29.371940252202688 19.89722856526911 78-79 20.38457908926306 30.22626619600914 29.5202126748632 19.868942039864603 80-81 20.471985955097963 30.205522915196198 29.388260849213697 19.934230280492145 82-83 20.370093899812776 30.44663055231296 29.465159450343915 19.718116097530352 84-85 20.39000996871807 30.234836574668968 29.435855776605912 19.939297680007048 86-87 20.31428736874192 30.434254413607114 29.331914735186093 19.91954348246487 88-89 20.306166650025666 30.27637758057353 29.456762145660182 19.96069362374062 90-91 20.38781427676947 30.23668828030777 29.34996028861145 20.025537154311312 92-93 20.27445687824789 30.316808310162653 29.47769676993325 19.93103804165621 94-95 20.362391178323936 30.215866821788268 29.349701645859234 20.072040354028566 96-97 20.372397847943645 30.30459001109294 29.313310598907382 20.009701542056032 98-99 20.3275532456198 30.28407609564452 29.386276184499984 20.002094474235697 100-101 20.564109395953693 30.109917911429402 29.03429413621248 20.291678556404424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 513.0 1 615.0 2 815.0 3 980.5 4 1183.5 5 1436.5 6 1717.5 7 2108.0 8 2599.0 9 3254.5 10 4261.0 11 5926.0 12 8414.5 13 11795.0 14 16570.0 15 22976.5 16 31503.0 17 41850.0 18 53765.5 19 67352.0 20 82826.5 21 98424.0 22 113382.5 23 128681.0 24 144371.5 25 158258.0 26 169922.5 27 181836.5 28 194070.0 29 208139.0 30 224688.5 31 246828.0 32 275603.0 33 309893.5 34 350778.5 35 398325.5 36 458587.0 37 517996.0 38 559044.0 39 579014.5 40 577879.5 41 552655.5 42 505859.0 43 450687.0 44 395275.0 45 352901.0 46 322492.5 47 299254.5 48 281626.0 49 265125.5 50 250048.5 51 232781.5 52 213201.0 53 194508.0 54 177583.5 55 162005.0 56 149463.0 57 137447.5 58 123433.0 59 108857.0 60 93482.5 61 77276.5 62 62095.5 63 50140.5 64 40542.0 65 32351.5 66 25787.0 67 20159.5 68 15406.0 69 11886.5 70 9075.5 71 6932.0 72 5472.0 73 4412.0 74 3543.5 75 2858.0 76 2261.5 77 1725.0 78 1455.5 79 1256.0 80 904.5 81 627.5 82 482.5 83 341.5 84 237.0 85 133.5 86 87.0 87 67.5 88 47.0 89 31.0 90 25.0 91 19.5 92 14.0 93 13.0 94 12.0 95 14.5 96 11.0 97 5.5 98 7.0 99 12.0 100 34.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.972209026826624E-4 2 3.4441032542155615E-4 3 0.001344040294328024 4 0.0017556526344659812 5 0.008769862920490357 6 0.01343200269144069 7 0.013331199669366087 8 0.009467083823173019 9 0.0027300818478537985 10-11 0.006111183213272734 12-13 0.01069352059174734 14-15 0.03326079715869882 16-17 0.014398031652988955 18-19 0.02155084609436591 20-21 0.017392721433788585 22-23 0.029623488112173604 24-25 0.025419162066478755 26-27 0.021214836020783903 28-29 0.028959868216849143 30-31 0.031752951953499566 32-33 0.03617148442110294 34-35 0.018056341329113046 36-37 0.02791403686282515 38-39 0.027725031196435268 40-41 0.031164934324731056 42-43 0.03894356752815449 44-45 0.02678420299040565 46-47 0.027305018604457762 48-49 0.021651649116440512 50-51 0.03500804954132525 52-53 0.02791403686282515 54-55 0.029951097933916062 56-57 0.023797913461445573 58-59 0.02516295438537247 60-61 0.03308859199598804 62-63 0.029766292393445956 64-65 0.029127873253640146 66-67 0.02486894557098822 68-69 0.026439792664984092 70-71 0.029808293652643706 72-73 0.03636049008749282 74-75 0.031513544776072384 76-77 0.033739611513553175 78-79 0.03506265117828233 80-81 0.04079162293285553 82-83 0.042618677707957686 84-85 0.04774283133008328 86-87 0.04875506167674907 88-89 0.05108193143630446 90-91 0.050863524888476154 92-93 0.051291937732293215 94-95 0.05218656455320531 96-97 0.05289218570772752 98-99 0.05402201958014701 100-101 0.05787773517450053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1904405E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.768643112045297 #Duplication Level Percentage of deduplicated Percentage of total 1 37.399999519264966 11.507472375989293 2 16.274283286364387 10.014752286849388 3 14.380801938223328 13.274332875066087 4 9.957736308920566 12.255441387721284 5 7.595587067664866 11.685295385572344 6 5.1073728521604265 9.428815951696382 7 3.1031584275501665 6.68359819241987 8 1.9800180714712452 4.873797551719916 9 1.1827681658632896 3.2752954421762355 >10 2.93944795448644 15.137483137348578 >50 0.07101892596878429 1.4540473069091453 >100 0.007287986857287014 0.30072760951315725 >500 5.194952042547398E-4 0.10894049701826027 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.240277023505164E-5 0.0 0.0 2 9.240277023505164E-5 0.0 0.0 3 9.240277023505164E-5 0.0 0.0 4 9.240277023505164E-5 0.0 0.0 5 9.240277023505164E-5 0.0 0.0 6 9.240277023505164E-5 0.0 0.0 7 1.008030220746018E-4 0.0 0.0 8 1.3440402943280238E-4 8.400251839550149E-6 1.6800503679100298E-5 9 1.3440402943280238E-4 8.400251839550149E-6 1.6800503679100298E-5 10-11 1.5960478495145283E-4 8.400251839550149E-6 2.520075551865045E-5 12-13 1.8900566638987835E-4 1.6800503679100298E-5 2.520075551865045E-5 14-15 2.184065478283039E-4 8.400251839550149E-5 2.520075551865045E-5 16-17 2.8980868846448016E-4 8.400251839550149E-5 2.520075551865045E-5 18-19 3.9061171053908196E-4 8.400251839550149E-5 7.560226655595134E-5 20-21 5.922177546882855E-4 1.0920327391415194E-4 1.1340339983392702E-4 22-23 7.896236729177141E-4 1.0920327391415194E-4 1.2600377759325226E-4 24-25 9.660289615482673E-4 1.3860415535257747E-4 1.2600377759325226E-4 26-27 0.0011592347538579206 2.4360730334695434E-4 1.2600377759325226E-4 28-29 0.001348240420247799 2.8560856254470507E-4 1.4280428127235253E-4 30-31 0.001625448730952954 2.8560856254470507E-4 1.8480554047010329E-4 32-33 0.0018144543973428323 2.8560856254470507E-4 2.1000629598875374E-4 34-35 0.002032860945171136 2.8560856254470507E-4 2.1000629598875374E-4 36-37 0.002335270011394942 2.8560856254470507E-4 2.520075551865045E-4 38-39 0.002709081218254923 2.8560856254470507E-4 2.520075551865045E-4 40-41 0.0030450912918369294 2.8560856254470507E-4 2.520075551865045E-4 42-43 0.00336010073582006 2.8980868846448016E-4 2.520075551865045E-4 44-45 0.003805314083316218 2.982089403040303E-4 2.520075551865045E-4 46-47 0.004271528060411251 3.0240906622380537E-4 2.688080588656048E-4 48-49 0.004561336748875731 3.0240906622380537E-4 2.8560856254470507E-4 50-51 0.004943548207575263 3.0240906622380537E-4 3.0660919214358046E-4 52-53 0.00535516054771322 3.0240906622380537E-4 3.4021019950178106E-4 54-55 0.005863375784006005 3.0240906622380537E-4 3.9481183645885705E-4 56-57 0.006253987494545086 3.0240906622380537E-4 3.9481183645885705E-4 58-59 0.006573197064447992 3.0240906622380537E-4 4.872146066939087E-4 60-61 0.006976409152746399 3.1080931806335555E-4 4.872146066939087E-4 62-63 0.007505625018638058 3.1500944398313063E-4 4.914147326136838E-4 64-65 0.008047441262289044 3.1920956990290567E-4 4.956148585334589E-4 66-67 0.008551456372662053 3.36010073582006E-4 5.292158658916595E-4 68-69 0.009143674127350338 3.4441032542155615E-4 5.292158658916595E-4 70-71 0.009777893141236374 3.4441032542155615E-4 5.796173769289603E-4 72-73 0.010475114043919036 3.4861045134133124E-4 5.880176287685105E-4 74-75 0.011151334317002824 3.5281057726110627E-4 6.216186361267111E-4 76-77 0.011924157486241437 3.7381120685998166E-4 6.468193916453615E-4 78-79 0.012726381536918477 3.9481183645885705E-4 6.720201471640119E-4 80-81 0.01378061314278202 3.9481183645885705E-4 6.720201471640119E-4 82-83 0.01479704361536759 4.0321208829840723E-4 7.098212804419876E-4 84-85 0.015918477235947533 4.1161234013795735E-4 7.476224137199633E-4 86-87 0.017111312997163654 4.368130956566078E-4 7.476224137199633E-4 88-89 0.01842595241005325 4.4521334749615796E-4 7.476224137199633E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATCGG 1115 0.0 42.586952 1 CATCGGT 1010 0.0 25.38794 2 CGGTGAC 1115 0.0 24.274868 5 ACAACGG 1225 0.0 24.032948 1 ATCGGAG 935 0.0 20.314375 3 ATCGGCG 165 6.34463E-5 20.145088 3 ATCGGTG 1335 0.0 19.563047 3 CAACGGT 970 0.0 16.644175 2 CGGAGAC 865 0.0 15.370882 5 TCCGATC 865 0.0 13.729221 32-33 TCGGAGA 1105 0.0 12.891815 4 CCGATCT 850 0.0 12.015492 32-33 TCGGTGA 1965 0.0 11.116047 4 GACCGGA 650 3.2747266E-8 10.961404 9 TCCGACG 240 1.5300475E-5 10.885328 18-19 ATCGGGG 790 4.4019544E-10 10.819333 3 CTTCCGA 1580 0.0 9.920827 30-31 CATCGGG 635 3.165691E-6 9.721307 2 CGATCTA 675 0.0 9.500382 34-35 CGGTAAC 490 0.0024279384 8.721744 5 >>END_MODULE