FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 1-58812_2015-03-25 191131308797.34200000004434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 1-58812_2015-03-25 191131308797.34200000004434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11904405
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCGG11150.042.5869521
CATCGGT10100.025.387942
CGGTGAC11150.024.2748685
ACAACGG12250.024.0329481
ATCGGAG9350.020.3143753
ATCGGCG1656.34463E-520.1450883
ATCGGTG13350.019.5630473
CAACGGT9700.016.6441752
CGGAGAC8650.015.3708825
TCCGATC8650.013.72922132-33
TCGGAGA11050.012.8918154
CCGATCT8500.012.01549232-33
TCGGTGA19650.011.1160474
GACCGGA6503.2747266E-810.9614049
TCCGACG2401.5300475E-510.88532818-19
ATCGGGG7904.4019544E-1010.8193333
CTTCCGA15800.09.92082730-31
CATCGGG6353.165691E-69.7213072
CGATCTA6750.09.50038234-35
CGGTAAC4900.00242793848.7217445
Produced by FastQC (version 0.11.2)