##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l02n01 9-9610.34100000001ba3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11329192
Sequences flagged as poor quality	0
Sequence length	101
%GC	30
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.95812331541384	38.0	38.0	38.0	32.0	38.0
2	36.96445086286824	38.0	38.0	38.0	32.0	38.0
3	36.94328262774609	38.0	38.0	38.0	32.0	38.0
4	36.93241918752899	38.0	38.0	38.0	32.0	38.0
5	36.90506101405996	38.0	38.0	38.0	32.0	38.0
6	38.81390393948659	40.0	38.0	40.0	38.0	40.0
7	38.759285304724294	40.0	38.0	40.0	38.0	40.0
8	38.743354248034635	40.0	38.0	40.0	38.0	40.0
9	38.73755374611005	40.0	38.0	40.0	38.0	40.0
10-11	38.752399641563144	40.0	38.0	40.0	38.0	40.0
12-13	38.74198685131296	40.0	38.0	40.0	38.0	40.0
14-15	38.71964390752668	40.0	38.0	40.0	38.0	40.0
16-17	38.70180839021883	40.0	38.0	40.0	38.0	40.0
18-19	38.66952444622706	40.0	38.0	40.0	38.0	40.0
20-21	38.645298402569225	40.0	38.0	40.0	38.0	40.0
22-23	38.629004213186604	40.0	38.0	40.0	38.0	40.0
24-25	38.585221876370355	40.0	38.0	40.0	38.0	40.0
26-27	38.513952451331036	40.0	38.0	40.0	38.0	40.0
28-29	38.48030415584801	40.0	38.0	40.0	38.0	40.0
30-31	38.435414017169094	40.0	38.0	40.0	38.0	40.0
32-33	38.40547106095474	40.0	38.0	40.0	38.0	40.0
34-35	38.376618253093426	40.0	38.0	40.0	38.0	40.0
36-37	38.34197222538024	40.0	38.0	40.0	38.0	40.0
38-39	38.29761663497273	40.0	38.0	40.0	38.0	40.0
40-41	38.26108949340782	40.0	38.0	40.0	38.0	40.0
42-43	38.22907026379286	40.0	38.0	40.0	38.0	40.0
44-45	38.20372542896263	40.0	38.0	40.0	38.0	40.0
46-47	38.17011032207769	40.0	38.0	40.0	38.0	40.0
48-49	38.129497496379265	40.0	38.0	40.0	38.0	40.0
50-51	38.081463046967514	40.0	38.0	40.0	38.0	40.0
52-53	38.04248030221396	40.0	38.0	40.0	38.0	40.0
54-55	37.9982396361541	40.0	38.0	40.0	38.0	40.0
56-57	37.94790872994297	40.0	38.0	40.0	38.0	40.0
58-59	37.92671622124508	40.0	38.0	40.0	38.0	40.0
60-61	37.87257440777772	40.0	38.0	40.0	35.0	40.0
62-63	37.84624521325087	40.0	38.0	40.0	32.0	40.0
64-65	37.807904967980065	40.0	38.0	40.0	32.0	40.0
66-67	37.724341462303755	40.0	38.0	40.0	32.0	40.0
68-69	37.67198543373614	40.0	38.0	40.0	32.0	40.0
70-71	37.65738227404037	40.0	38.0	40.0	32.0	40.0
72-73	37.613620106358866	40.0	38.0	40.0	32.0	40.0
74-75	37.544704732694086	40.0	38.0	40.0	32.0	40.0
76-77	37.481399953324114	40.0	38.0	40.0	32.0	40.0
78-79	37.40030008318334	40.0	38.0	40.0	32.0	40.0
80-81	37.36179680775116	40.0	38.0	40.0	32.0	40.0
82-83	37.29532763678115	39.0	38.0	40.0	32.0	40.0
84-85	37.23373974948964	38.0	38.0	40.0	32.0	40.0
86-87	37.17833681342853	38.0	38.0	40.0	32.0	40.0
88-89	37.133753139676685	38.0	38.0	40.0	32.0	40.0
90-91	37.07931695393634	38.0	38.0	40.0	32.0	40.0
92-93	37.01673985223306	38.0	38.0	40.0	32.0	40.0
94-95	36.93678878423104	38.0	38.0	40.0	32.0	40.0
96-97	36.862970236535844	38.0	38.0	40.0	32.0	40.0
98-99	36.762471586676256	38.0	38.0	40.0	32.0	40.0
100-101	35.96167987973017	38.0	38.0	40.0	29.5	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.07206713052515568
2201	2	0.06648478040722239
2201	3	0.0889874572301892
2201	4	0.013808401411502302
2201	5	0.04521392160299342
2201	6	0.13875517993476194
2201	7	0.17373975718612655
2201	8	0.15179343812895496
2201	9	0.13746852270438836
2201	10-11	0.09122361976019278
2201	12-13	0.026964538997617638
2201	14-15	0.06214921880778945
2201	16-17	0.08084660191775583
2201	18-19	0.037162043691374436
2201	20-21	0.08358860682052693
2201	22-23	0.0831471902343921
2201	24-25	0.03783907905787487
2201	26-27	0.02870503294662541
2201	28-29	0.057878823665113543
2201	30-31	0.015421451168940337
2201	32-33	0.0018230554932046061
2201	34-35	0.007862028523554443
2201	36-37	0.014556353146247147
2201	38-39	0.02671654409916613
2201	40-41	0.02014750635176199
2201	42-43	-0.06765945373393834
2201	44-45	-0.03948045123972577
2201	46-47	-0.025912213993194655
2201	48-49	-0.04665450208531752
2201	50-51	-0.08289470331558135
2201	52-53	-0.030197935843666812
2201	54-55	-0.1488890339046094
2201	56-57	-0.1153234364841822
2201	58-59	-0.025975016520625616
2201	60-61	-0.06514524907304775
2201	62-63	-0.11790824586712745
2201	64-65	-0.1270758486703869
2201	66-67	-0.07293121936087488
2201	68-69	-0.06978620657777412
2201	70-71	-0.05277160836867978
2201	72-73	-0.10028459097345888
2201	74-75	-0.0926602076723384
2201	76-77	-0.09818097880050658
2201	78-79	-0.12264646126030243
2201	80-81	-0.17333407678142976
2201	82-83	-0.20104050722359545
2201	84-85	-0.2108629246615834
2201	86-87	-0.2577903738706695
2201	88-89	-0.24150219719599875
2201	90-91	-0.24677042459330778
2201	92-93	-0.2785679891476178
2201	94-95	-0.2878043623464137
2201	96-97	-0.231887556633545
2201	98-99	-0.2945771312523746
2201	100-101	-0.4040148582507328
1101	1	0.09433777267567223
1101	2	0.09565525770640448
1101	3	0.08701729947399883
1101	4	0.05138568197467919
1101	5	0.01925448664923124
1101	6	0.1065329675803568
1101	7	0.163000591790194
1101	8	0.08796863802967181
1101	9	0.04554222608529557
1101	10-11	0.08248885528896466
1101	12-13	0.06253220212959576
1101	14-15	0.06854120666520913
1101	16-17	0.0414618551319208
1101	18-19	0.05285217640481221
1101	20-21	0.04699107512593059
1101	22-23	0.04569174706397661
1101	24-25	0.033138812131639384
1101	26-27	0.004612576864204243
1101	28-29	0.021912799652128
1101	30-31	-0.004089751702650801
1101	32-33	-0.03362856121682967
1101	34-35	-0.006336785594029948
1101	36-37	0.016445446576852873
1101	38-39	0.0014123505417273918
1101	40-41	0.03681288255850035
1101	42-43	0.002145109757663022
1101	44-45	-0.04267500784948908
1101	46-47	-0.1189048599745206
1101	48-49	-0.06291593954797747
1101	50-51	-0.07726920835262518
1101	52-53	-0.027064578213263246
1101	54-55	-0.08229153783606336
1101	56-57	-0.06971968277135687
1101	58-59	-0.045749446811520045
1101	60-61	-0.10935485204719697
1101	62-63	-0.03649572542784085
1101	64-65	-0.144893711455353
1101	66-67	-0.09351082854752946
1101	68-69	-0.09829785671151825
1101	70-71	-0.1331389743988396
1101	72-73	-0.13800130189866167
1101	74-75	-0.1160359871074661
1101	76-77	-0.15044753627818608
1101	78-79	-0.09712506460304837
1101	80-81	-0.13428194714334296
1101	82-83	-0.27942506106819565
1101	84-85	-0.18631647268352935
1101	86-87	-0.18049852988738024
1101	88-89	-0.26943604279490074
1101	90-91	-0.25330191925356615
1101	92-93	-0.250839218039296
1101	94-95	-0.33034679578914705
1101	96-97	-0.2890646002264816
1101	98-99	-0.38167446241028813
1101	100-101	-0.26662504670036924
2202	1	0.08360134405279496
2202	2	0.025531938415298328
2202	3	-0.005861723510939498
2202	4	0.017580891119763464
2202	5	-0.002040067153103564
2202	6	0.04320010898214832
2202	7	0.04666149048875923
2202	8	0.011076422875056835
2202	9	0.01846200039014434
2202	10-11	0.02217462972446782
2202	12-13	-0.021988114847843576
2202	14-15	-0.0210442452060704
2202	16-17	0.002379191486475918
2202	18-19	-0.05328156505948556
2202	20-21	-0.13071433112236974
2202	22-23	-0.09559302843321404
2202	24-25	-0.0336663820989358
2202	26-27	-0.06875521914172111
2202	28-29	-0.0630309216535565
2202	30-31	-0.07869558888272365
2202	32-33	-0.1648584561124835
2202	34-35	-0.20737099414741067
2202	36-37	-0.12202150655237176
2202	38-39	-0.11093950646875328
2202	40-41	-0.10967960581179881
2202	42-43	-0.18423723667381608
2202	44-45	-0.17753216642752534
2202	46-47	-0.22551446039275902
2202	48-49	-0.23370424908392806
2202	50-51	-0.2608306690768032
2202	52-53	-0.2354052087193068
2202	54-55	-0.21894964755983182
2202	56-57	-0.26506625528755734
2202	58-59	-0.227383763452643
2202	60-61	-0.20275994621138693
2202	62-63	-0.3044325433026316
2202	64-65	-0.34258843029296315
2202	66-67	-0.24734400238853738
2202	68-69	-0.2695820246239151
2202	70-71	-0.26338847700092316
2202	72-73	-0.2627874872224396
2202	74-75	-0.2743336586650287
2202	76-77	-0.34286830708823146
2202	78-79	-0.29643475705056943
2202	80-81	-0.4506815379261937
2202	82-83	-0.3801091713187361
2202	84-85	-0.3383274454301173
2202	86-87	-0.3558028673658171
2202	88-89	-0.3669082934724628
2202	90-91	-0.45069561214554454
2202	92-93	-0.40727521145971224
2202	94-95	-0.4545888762663637
2202	96-97	-0.5546790942461115
2202	98-99	-0.6059054617859729
2202	100-101	-0.564964963281902
1102	1	0.06538406279980791
1102	2	0.041608417580924595
1102	3	0.018791466475818197
1102	4	-0.042918397421004784
1102	5	-0.04091878462659082
1102	6	-0.060711450172902914
1102	7	-0.060815078957475066
1102	8	-0.02992584868059822
1102	9	-0.015527726945308018
1102	10-11	-0.07374987070826222
1102	12-13	-0.0733558544960573
1102	14-15	-0.07934925621800204
1102	16-17	-0.06260701653596357
1102	18-19	-0.1376872226295731
1102	20-21	-0.1011111204439672
1102	22-23	-0.13611610579377498
1102	24-25	-0.11887412933043606
1102	26-27	-0.16070618553199978
1102	28-29	-0.20803828469667707
1102	30-31	-0.19434696854447964
1102	32-33	-0.15177860031746349
1102	34-35	-0.14412041247334173
1102	36-37	-0.18049745390877092
1102	38-39	-0.19239183514888225
1102	40-41	-0.2323256544883705
1102	42-43	-0.2719419871141042
1102	44-45	-0.3237469428373103
1102	46-47	-0.3171660844505766
1102	48-49	-0.3170863026948538
1102	50-51	-0.3409836730611602
1102	52-53	-0.33613019339298944
1102	54-55	-0.39231569709787806
1102	56-57	-0.4279603010389721
1102	58-59	-0.36744716358622753
1102	60-61	-0.35945985123428414
1102	62-63	-0.41643703433866364
1102	64-65	-0.4946625373698055
1102	66-67	-0.38485205865350025
1102	68-69	-0.4219966991949349
1102	70-71	-0.47870291191213
1102	72-73	-0.5136363794895118
1102	74-75	-0.5616086219425682
1102	76-77	-0.5198695559188948
1102	78-79	-0.4981216645200277
1102	80-81	-0.501840762221434
1102	82-83	-0.595617126198114
1102	84-85	-0.5279974865854413
1102	86-87	-0.5234229681301272
1102	88-89	-0.5561420373920924
1102	90-91	-0.633036152140086
1102	92-93	-0.6531426435214414
1102	94-95	-0.7191973768262514
1102	96-97	-0.795968240984557
1102	98-99	-0.8539605239379142
1102	100-101	-0.9091142475627834
2203	1	0.10232320866086297
2203	2	0.06605538347896811
2203	3	0.030885363872627636
2203	4	-0.004980563045634767
2203	5	0.04223886344068717
2203	6	-0.13237949386247294
2203	7	-0.06147399782116736
2203	8	-0.13042945621860724
2203	9	-0.029241459905044564
2203	10-11	-0.08374041667935472
2203	12-13	-0.08595381924293122
2203	14-15	-0.08343258335012393
2203	16-17	-0.15631799553607806
2203	18-19	-0.18888287790812086
2203	20-21	-0.16240941542638865
2203	22-23	-0.0856637774270439
2203	24-25	-0.12479496369135035
2203	26-27	-0.18836342819760432
2203	28-29	-0.15005527342053426
2203	30-31	-0.1459181299631922
2203	32-33	-0.17142632636456767
2203	34-35	-0.23288593828380755
2203	36-37	-0.2499932454692413
2203	38-39	-0.21497748227630353
2203	40-41	-0.18983198114889177
2203	42-43	-0.20431337078709788
2203	44-45	-0.2821045229609993
2203	46-47	-0.3128507202965807
2203	48-49	-0.3107982564792877
2203	50-51	-0.27534879232710097
2203	52-53	-0.236427182422986
2203	54-55	-0.39230405312247996
2203	56-57	-0.33015692579267153
2203	58-59	-0.27128771770982496
2203	60-61	-0.2918330793267643
2203	62-63	-0.38102513461296184
2203	64-65	-0.3850174869375991
2203	66-67	-0.3941676181953042
2203	68-69	-0.44895827897160245
2203	70-71	-0.5143562603703913
2203	72-73	-0.4924855851471648
2203	74-75	-0.5210095832781434
2203	76-77	-0.46445779254232633
2203	78-79	-0.4733323541100418
2203	80-81	-0.5033141668214185
2203	82-83	-0.5752891131703777
2203	84-85	-0.6235111206119015
2203	86-87	-0.5854867144394973
2203	88-89	-0.6656819817695805
2203	90-91	-0.6991719888436663
2203	92-93	-0.6694997398900995
2203	94-95	-0.6568482044457156
2203	96-97	-0.76308036900285
2203	98-99	-0.7586511485967691
2203	100-101	-0.8136475255704312
1103	1	0.09746990704326919
1103	2	0.09496889439534328
1103	3	0.07018938630015015
1103	4	0.06390037947109306
1103	5	0.0344342467680292
1103	6	-0.005384950743348327
1103	7	0.006202156497387534
1103	8	0.007895042557066745
1103	9	-0.0328334454946031
1103	10-11	0.0328755073509015
1103	12-13	-0.01687350087976114
1103	14-15	-0.020381232671738303
1103	16-17	-0.005657589302046517
1103	18-19	0.009732155931423847
1103	20-21	-0.004640013401250087
1103	22-23	-0.026233885929094924
1103	24-25	0.016599177697671053
1103	26-27	0.0027489502397770593
1103	28-29	-0.07566455122960747
1103	30-31	-0.04417459133281909
1103	32-33	-0.08897168365444941
1103	34-35	-0.07775785550640535
1103	36-37	-0.08267859299802183
1103	38-39	-0.0474988124595086
1103	40-41	-0.03862946844925119
1103	42-43	-0.0652847997452426
1103	44-45	-0.10054537229213878
1103	46-47	-0.12756221225727415
1103	48-49	-0.10758810510108674
1103	50-51	-0.15233986171462988
1103	52-53	-0.13908697865340258
1103	54-55	-0.11028713492168407
1103	56-57	-0.10663546529139722
1103	58-59	-0.11858339232713178
1103	60-61	-0.14643817996134345
1103	62-63	-0.18953524737306537
1103	64-65	-0.21307151655778966
1103	66-67	-0.18663365997607428
1103	68-69	-0.21141561991294822
1103	70-71	-0.2501874650010052
1103	72-73	-0.22203705953943143
1103	74-75	-0.2512111604078626
1103	76-77	-0.24852467827150093
1103	78-79	-0.29082744936449956
1103	80-81	-0.2869881367267695
1103	82-83	-0.3371904527197458
1103	84-85	-0.3326342170396899
1103	86-87	-0.37542358537530873
1103	88-89	-0.3208758692835758
1103	90-91	-0.38852142806404544
1103	92-93	-0.3208898504427111
1103	94-95	-0.439420993262722
1103	96-97	-0.4531315510458711
1103	98-99	-0.35523855794954073
1103	100-101	-0.3711069693862754
2204	1	0.07112157048010204
2204	2	0.05708452761658123
2204	3	0.08777936925935137
2204	4	0.08539304409171677
2204	5	0.0830887717618225
2204	6	0.06180790319911722
2204	7	0.08400030777856671
2204	8	0.09120635822444711
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1212	96-97	0.3799186142378801
1212	98-99	0.35065290790092263
1212	100-101	0.4516356619086537
1213	1	-0.31225787618759426
1213	2	-0.18188089361605364
1213	3	-0.14283597848838525
1213	4	-0.14651940567743793
1213	5	-0.08557045083578885
1213	6	-0.016772709253565665
1213	7	-0.03792257255254583
1213	8	-0.05674236718304826
1213	9	-0.04640502175369221
1213	10-11	-0.08833388706150913
1213	12-13	-0.09349559711304067
1213	14-15	-0.1750684415566255
1213	16-17	-0.25025770506645273
1213	18-19	-0.27532756384827195
1213	20-21	-0.11157499447450903
1213	22-23	-0.17933527578363595
1213	24-25	-0.2511194256087208
1213	26-27	-0.10361002905668215
1213	28-29	-0.11138534656177512
1213	30-31	-0.07146698223706949
1213	32-33	-0.0913545701757954
1213	34-35	-0.0016523480061465534
1213	36-37	0.021473858730828965
1213	38-39	0.05193768525246867
1213	40-41	0.039041343696574415
1213	42-43	0.09939455613204018
1213	44-45	0.06298077296890625
1213	46-47	0.1048781190856829
1213	48-49	0.11367874549352308
1213	50-51	0.11479751678385952
1213	52-53	0.1761703614235799
1213	54-55	0.22943871132863336
1213	56-57	0.21660371999526973
1213	58-59	0.18303467373696236
1213	60-61	0.16879549316723086
1213	62-63	0.23761290948486646
1213	64-65	0.22253784843069013
1213	66-67	0.27332773390011056
1213	68-69	0.25172727585591304
1213	70-71	0.2864601016742938
1213	72-73	0.14355527024484616
1213	74-75	0.23250162230040416
1213	76-77	0.30694756155963177
1213	78-79	0.34146095397278486
1213	80-81	0.3025126363613637
1213	82-83	0.43943046769251026
1213	84-85	0.3189509459166189
1213	86-87	0.3513851880672618
1213	88-89	0.34788876315590755
1213	90-91	0.42405668451030465
1213	92-93	0.41597425759194806
1213	94-95	0.5172486015959308
1213	96-97	0.45875813251626596
1213	98-99	0.5138281866507697
1213	100-101	0.4638837490119698
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	19.0
14	32.0
15	85.0
16	165.0
17	339.0
18	701.0
19	1575.0
20	3265.0
21	5973.0
22	10560.0
23	16975.0
24	25550.0
25	36832.0
26	50882.0
27	68436.0
28	87499.0
29	110740.0
30	135204.0
31	163562.0
32	198209.0
33	240698.0
34	298980.0
35	387488.0
36	540879.0
37	869202.0
38	1901205.0
39	6174137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.370993025217256	35.27706962643006	35.85279711388788	15.499140234464804
2	14.135399577258086	36.70704776404554	35.039648163484216	14.117904495212155
3	14.037214657497199	35.15427225524998	35.49857748019453	15.309935607058298
4	14.577553280057396	34.82012662509383	35.589943219251644	15.01237687559713
5	15.053174136337349	35.07132724028333	35.30638372092202	14.569114902457297
6	14.882261682916134	35.45065702832117	34.46368461228303	15.203396676479663
7	14.956238714993972	35.22132028480054	35.13868420625231	14.68375679395318
8	14.635200815733374	35.45654447378065	35.057133818545935	14.851120891940042
9	14.591002867196032	34.829630758147076	35.480957283166035	15.098409091490856
10-11	14.663736653277649	35.2082576806489	35.18642027767926	14.941585388394188
12-13	14.475895544889697	35.12956175515429	35.37092495210603	15.023617747849979
14-15	14.526813392838228	35.00731747791516	35.31736581387302	15.148503315373588
16-17	14.618703610990085	34.916139650559366	35.41469682921783	15.050459909232716
18-19	14.690134124304716	34.76098295447725	35.28086557276106	15.268017348456977
20-21	14.762906095073767	34.659046875582014	35.19064306672803	15.387403962616188
22-23	14.758849187266577	34.74148075729426	35.12878165190478	15.37088840353438
24-25	14.870071656312033	34.59649681527257	35.0854832558687	15.447948272546697
26-27	14.9531734374669	34.63976647624159	34.997374005211384	15.409686081080126
28-29	14.972515214279818	34.61582426331727	34.95617219356174	15.455488328841172
30-31	15.007733633748611	34.50319485468955	34.933519879157146	15.555551632404688
32-33	15.077910180220297	34.62648489323837	34.84719829519994	15.448406631341399
34-35	15.056041256547958	34.6495383469469	34.84938335771573	15.445037038789414
36-37	15.052535532329623	34.45120421228789	34.84053612076325	15.655724134619241
38-39	15.158057725161388	34.54252011791238	34.81953080016358	15.479891356762648
40-41	15.160859268521282	34.61042507645557	34.725923900104	15.502791754919151
42-43	15.082203251052839	34.43265477636046	34.78530753995841	15.699834432628293
44-45	15.160171632263292	34.48689001625605	34.76174829445647	15.591190057024187
46-47	15.191762945215412	34.595210765217765	34.697010860869156	15.516015428697669
48-49	15.147125372927196	34.38721553404092	34.746984226925484	15.718674866106403
50-51	15.239211782868406	34.408540306357224	34.69410293788216	15.658144972892208
52-53	15.214636045263722	34.54980796699175	34.69856297803825	15.536993009706288
54-55	15.186284855274025	34.394299331447215	34.71799509556688	15.701420717711887
56-57	15.272528753470976	34.4129402934557	34.65309474233595	15.661436210737373
58-59	15.276577811115445	34.44025752081527	34.733494699484744	15.549669968584537
60-61	15.28469586202553	34.44025672768812	34.5769782525959	15.69806915769045
62-63	15.24298832741016	34.37377841821011	34.667653178439686	15.715580075940045
64-65	15.254008389954635	34.43258971871969	34.671513340792266	15.641888550533409
66-67	15.30829722137802	34.40612755584418	34.62136931520823	15.664205907569578
68-69	15.317242922225658	34.41054933174257	34.61467148796706	15.657536258064711
70-71	15.306567452324899	34.44499579542771	34.57858966380061	15.669847088446783
72-73	15.29212838535895	34.37053360122327	34.59578168243883	15.741556330978945
74-75	15.340094751427024	34.41575698761208	34.57508112417268	15.66906713678822
76-77	15.329182520211296	34.47299023086269	34.59072546723799	15.607101781688021
78-79	15.356212583094417	34.34636364459063	34.588056741151554	15.709367031163401
80-81	15.402502431786813	34.415813209109295	34.57650359819696	15.605180760906933
82-83	15.343652351424064	34.498992997336146	34.54538310690121	15.61197154433858
84-85	15.294068367569613	34.32203584339753	34.59636188173247	15.78753390730039
86-87	15.35478445369809	34.50354612746566	34.52860477494139	15.613064643894859
88-89	15.376659144952084	34.526514871477985	34.545860206451756	15.550965777118172
90-91	15.303865235513495	34.349940351944056	34.5651623839926	15.781032028549857
92-93	15.383899649574017	34.39485452782051	34.573746525633496	15.647499296971972
94-95	15.357714161922386	34.47071406353582	34.559125137334	15.612446637207785
96-97	15.357001555565795	34.411120097412216	34.53762567801112	15.694252669010872
98-99	15.402743309503677	34.4000572374298	34.52139105492141	15.675808398145117
100-101	15.394849826322075	34.50073245549304	34.550383722646345	15.554033995538536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1241.0
1	1159.0
2	1105.5
3	1297.0
4	1664.0
5	2182.0
6	2824.0
7	3730.0
8	5067.5
9	7107.0
10	10004.0
11	14405.0
12	21530.5
13	32494.5
14	48648.5
15	71471.5
16	102458.0
17	141770.5
18	188348.5
19	240618.5
20	295864.5
21	349529.5
22	401373.5
23	446716.5
24	482293.5
25	507211.0
26	518608.0
27	521162.0
28	523792.5
29	523135.5
30	516541.0
31	507620.5
32	492425.0
33	465959.0
34	428505.0
35	381195.5
36	332810.5
37	292554.5
38	257996.0
39	230901.5
40	210018.0
41	192614.0
42	177736.0
43	162668.0
44	147408.5
45	133277.5
46	121222.0
47	116991.5
48	108567.0
49	91537.5
50	75457.0
51	61913.0
52	51466.0
53	43480.5
54	37943.0
55	33269.5
56	29940.0
57	26818.5
58	23787.0
59	20911.0
60	17692.5
61	14397.0
62	11623.0
63	9303.5
64	7314.0
65	5848.0
66	4675.0
67	3614.5
68	2719.0
69	2079.5
70	1589.5
71	1228.0
72	943.5
73	767.5
74	653.0
75	572.0
76	491.0
77	416.0
78	365.5
79	295.5
80	256.5
81	205.5
82	145.5
83	109.0
84	72.0
85	46.5
86	23.5
87	7.0
88	3.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09700603538187012
2	0.0025950659146742328
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.413377405908559E-5
10-11	5.2960528870902714E-5
12-13	0.0
14-15	0.0013416667313962019
16-17	0.0
18-19	0.0
20-21	8.826754811817119E-5
22-23	1.4122807698907389E-4
24-25	3.839638343140446E-4
26-27	0.0013416667313962019
28-29	0.0017388706979279721
30-31	0.003791091191675452
32-33	0.004179468403395405
34-35	0.0058521384402347495
36-37	0.007162911529789592
38-39	0.011382100329838175
40-41	0.012101480847001269
42-43	0.013037116857053882
44-45	0.015164364766701808
46-47	0.01411839432150148
48-49	0.016894408709817964
50-51	0.015265872447037707
52-53	0.01626329574077304
54-55	0.016673739839522535
56-57	0.014917215631970931
58-59	0.011748410654528585
60-61	0.0060948741975597204
62-63	0.003411540734767316
64-65	4.280976083731302E-4
66-67	0.0026745067079805866
68-69	0.0038528784753581718
70-71	0.004002933307159063
72-73	0.016801727784293886
74-75	0.011841091580052664
76-77	0.03336954656607461
78-79	0.02510770406221379
80-81	0.018800987749170463
82-83	0.03091129535098355
84-85	0.011015790005147763
86-87	0.015579222242857214
88-89	0.007763130856993156
90-91	0.005812418043581572
92-93	0.012423657397632595
94-95	0.0125825389842453
96-97	0.007396820532302745
98-99	0.008301562900514
100-101	0.0015535088468798127
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.1329192E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.9138421164065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.28105063863113	39.04871352633859
2	20.86852998749128	24.588905617691115
3	6.940964928631656	12.267567358227602
4	2.6727209854749554	6.298410486379116
5	1.3198570808808863	3.887892583961885
6	0.5412901545736178	1.9133689623429238
7	0.29425039127599206	1.2134794766027244
8	0.1805575568999775	0.8509871520103485
9	0.12985847405353038	0.6885415474080443
>10	0.7417324607688329	8.063633881768647
>50	0.02725264762026262	1.0348938433403616
>100	0.0019346936979043965	0.1436055639287314
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.413377405908559E-5	0.0	2.6480264435451354E-5
2	4.413377405908559E-5	0.0	2.6480264435451354E-5
3	4.413377405908559E-5	0.0	2.6480264435451354E-5
4	4.413377405908559E-5	0.0	2.6480264435451354E-5
5	4.413377405908559E-5	0.0	3.530701924726847E-5
6	4.413377405908559E-5	0.0	3.530701924726847E-5
7	4.413377405908559E-5	0.0	3.530701924726847E-5
8	5.296052887090271E-5	8.826754811817118E-6	3.530701924726847E-5
9	5.296052887090271E-5	8.826754811817118E-6	3.530701924726847E-5
10-11	7.061403849453694E-5	8.826754811817118E-6	3.972039665317703E-5
12-13	7.061403849453694E-5	8.826754811817118E-6	8.826754811817119E-5
14-15	8.826754811817119E-5	1.3240132217725677E-5	9.268092552407974E-5
16-17	9.268092552407974E-5	2.2066887029542796E-5	1.0592105774180541E-4
18-19	1.0592105774180541E-4	2.6480264435451354E-5	1.1916118995953109E-4
20-21	1.7653509623634237E-4	2.6480264435451354E-5	1.279879447713482E-4
22-23	2.383223799190622E-4	3.089364184135991E-5	1.5888158661270813E-4
24-25	2.912829087899649E-4	3.530701924726847E-5	1.6770834142452524E-4
26-27	3.265899280372334E-4	4.8547151464994147E-5	1.7653509623634237E-4
28-29	3.972039665317703E-4	5.296052887090271E-5	1.8536185104815948E-4
30-31	4.898848920558501E-4	5.296052887090271E-5	1.8536185104815948E-4
32-33	6.046327046094726E-4	5.296052887090271E-5	1.8977522845406805E-4
34-35	7.414474041926379E-4	5.296052887090271E-5	1.9860198326588515E-4
36-37	8.385417071226262E-4	6.178728368271982E-5	2.1184211548361083E-4
38-39	0.0010062500485471513	6.620066108862838E-5	2.162554928895194E-4
40-41	0.0011695450125657682	7.061403849453694E-5	2.2508224770133653E-4
42-43	0.0012666393154957566	7.061403849453694E-5	2.2949562510724507E-4
44-45	0.0014211075247025562	7.502741590044551E-5	2.339090025131536E-4
46-47	0.0016197095079684412	7.944079330635406E-5	2.7804277657223923E-4
48-49	0.0018050713590166008	7.944079330635406E-5	3.0010966360178204E-4
50-51	0.0019948465874706687	7.944079330635406E-5	3.133497958195077E-4
52-53	0.002171381683707011	7.944079330635406E-5	3.1776317322541626E-4
54-55	0.002383223799190622	7.944079330635406E-5	3.265899280372334E-4
56-57	0.0025729990276446898	8.385417071226262E-5	3.442434376608676E-4
58-59	0.0028157347849696607	8.826754811817119E-5	3.4865681506677615E-4
60-61	0.003195285241877797	9.709430292998829E-5	3.618969472845018E-4
62-63	0.00342919424439095	9.709430292998829E-5	3.618969472845018E-4
64-65	0.003742544040210458	9.709430292998829E-5	3.751370795022275E-4
66-67	0.004055893836029965	9.709430292998829E-5	3.8396383431404463E-4
68-69	0.004298629593354936	9.709430292998829E-5	3.972039665317703E-4
70-71	0.004607566011768536	9.709430292998829E-5	4.0603072134358744E-4
72-73	0.00496063620424122	9.709430292998829E-5	4.192708535613131E-4
74-75	0.005424040831861619	1.0592105774180541E-4	4.2368423096722166E-4
76-77	0.005962472875382463	1.0592105774180541E-4	4.325109857790388E-4
78-79	0.006721573789198736	1.2357456736543964E-4	4.5016449540267306E-4
80-81	0.007493914835232734	1.2357456736543964E-4	4.678180050263073E-4
82-83	0.008345696674573085	1.2357456736543964E-4	4.7664475983812436E-4
84-85	0.009537308574168396	1.3240132217725678E-4	4.7664475983812436E-4
86-87	0.010786294380040518	1.3240132217725678E-4	4.854715146499415E-4
88-89	0.012494271436127132	1.3240132217725678E-4	4.942982694617586E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	1000	0.0	19.946054	5
GCCGTCT	1025	0.0	19.459568	6
TATGCCG	1305	0.0	15.284334	3
ATGCCGT	1340	0.0	15.239524	4
CGTATGC	1955	0.0	10.697678	1
GTATGCC	2660	0.0	8.391345	2
CCGTCTT	2860	0.0	7.306247	7
>>END_MODULE