##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n01 8-59796.34100000001b89.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10575190 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.7648920728611 38.0 38.0 38.0 32.0 38.0 2 36.75657780143903 38.0 38.0 38.0 32.0 38.0 3 36.74691877876426 38.0 38.0 38.0 32.0 38.0 4 36.744583879816815 38.0 38.0 38.0 32.0 38.0 5 36.732179185433075 38.0 38.0 38.0 32.0 38.0 6 38.677593404941184 40.0 38.0 40.0 38.0 40.0 7 38.628030323805056 40.0 38.0 40.0 38.0 40.0 8 38.61937950996625 40.0 38.0 40.0 38.0 40.0 9 38.618013482500075 40.0 38.0 40.0 38.0 40.0 10-11 38.632950708214224 40.0 38.0 40.0 38.0 40.0 12-13 38.624415400574364 40.0 38.0 40.0 38.0 40.0 14-15 38.61007584733702 40.0 38.0 40.0 38.0 40.0 16-17 38.59288740911511 40.0 38.0 40.0 38.0 40.0 18-19 38.563770532728014 40.0 38.0 40.0 38.0 40.0 20-21 38.54017369900683 40.0 38.0 40.0 38.0 40.0 22-23 38.52133011321782 40.0 38.0 40.0 38.0 40.0 24-25 38.4787557008432 40.0 38.0 40.0 38.0 40.0 26-27 38.402891248289635 40.0 38.0 40.0 38.0 40.0 28-29 38.36358372757369 40.0 38.0 40.0 38.0 40.0 30-31 38.31345555020761 40.0 38.0 40.0 38.0 40.0 32-33 38.2777370430224 40.0 38.0 40.0 38.0 40.0 34-35 38.24672322672217 40.0 38.0 40.0 38.0 40.0 36-37 38.20664716189496 40.0 38.0 40.0 38.0 40.0 38-39 38.15773262702609 40.0 38.0 40.0 38.0 40.0 40-41 38.111259986818204 40.0 38.0 40.0 38.0 40.0 42-43 38.07066459326026 40.0 38.0 40.0 38.0 40.0 44-45 38.047253288120594 40.0 38.0 40.0 38.0 40.0 46-47 38.00991570837026 40.0 38.0 40.0 38.0 40.0 48-49 37.96266483155385 40.0 38.0 40.0 38.0 40.0 50-51 37.90924579132857 40.0 38.0 40.0 35.0 40.0 52-53 37.862206541915555 40.0 38.0 40.0 32.0 40.0 54-55 37.81119195021555 40.0 38.0 40.0 32.0 40.0 56-57 37.7541461193605 40.0 38.0 40.0 32.0 40.0 58-59 37.729414837936716 40.0 38.0 40.0 32.0 40.0 60-61 37.66270275049432 40.0 38.0 40.0 32.0 40.0 62-63 37.626279575118744 40.0 38.0 40.0 32.0 40.0 64-65 37.581183978727566 40.0 38.0 40.0 32.0 40.0 66-67 37.48769946450135 40.0 38.0 40.0 32.0 40.0 68-69 37.42871541787902 40.0 38.0 40.0 32.0 40.0 70-71 37.40464308442685 40.0 38.0 40.0 32.0 40.0 72-73 37.35262075669562 40.0 38.0 40.0 32.0 40.0 74-75 37.27625456374779 38.0 38.0 40.0 32.0 40.0 76-77 37.202202182655824 38.0 38.0 40.0 32.0 40.0 78-79 37.114546783556605 38.0 38.0 40.0 32.0 40.0 80-81 37.06616566699984 38.0 38.0 40.0 32.0 40.0 82-83 36.98230121633748 38.0 38.0 40.0 32.0 40.0 84-85 36.91141743079793 38.0 38.0 40.0 32.0 40.0 86-87 36.85051512076851 38.0 38.0 40.0 32.0 40.0 88-89 36.799327860776025 38.0 38.0 40.0 32.0 40.0 90-91 36.730408673508464 38.0 38.0 40.0 32.0 40.0 92-93 36.65722020124461 38.0 38.0 40.0 32.0 40.0 94-95 36.56974361689956 38.0 38.0 40.0 32.0 40.0 96-97 36.47763316782016 38.0 38.0 40.0 32.0 40.0 98-99 36.37186788133357 38.0 38.0 40.0 32.0 40.0 100-101 35.48326271206474 38.0 35.0 39.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.1172338048655206 2201 2 0.1603344678164973 2201 3 0.12378705466953477 2201 4 0.06061186974731925 2201 5 0.0724529842999928 2201 6 0.1368747153146117 2201 7 0.2119696329727887 2201 8 0.1744645320899707 2201 9 0.19044495307213083 2201 10-11 0.11388446914941852 2201 12-13 0.06304428616744673 2201 14-15 0.07572055795397148 2201 16-17 0.10419233003553785 2201 18-19 0.05422844063744492 2201 20-21 0.12708952769222748 2201 22-23 0.14281553883116516 2201 24-25 0.0903360398626063 2201 26-27 0.08446267334826985 2201 28-29 0.06021831819439427 2201 30-31 0.07996399771265317 2201 32-33 0.03338919377844718 2201 34-35 0.03362224133809377 2201 36-37 0.07906115403850578 2201 38-39 0.0683189634879966 2201 40-41 0.08390852054706244 2201 42-43 0.004131392187780136 2201 44-45 -0.03227450973052726 2201 46-47 -0.008208073468630062 2201 48-49 0.028942182840232533 2201 50-51 -0.013937360649883601 2201 52-53 0.0024964008217693845 2201 54-55 -0.10773199848045323 2201 56-57 -0.07558481447816945 2201 58-59 -0.039013916656536196 2201 60-61 -0.028084253292526284 2201 62-63 -0.07396933817110352 2201 64-65 -0.09173378848063862 2201 66-67 -0.06542717977246326 2201 68-69 -0.07707197450429248 2201 70-71 -0.007567910508349485 2201 72-73 -0.08832221431072185 2201 74-75 0.004351499804783998 2201 76-77 -0.01857576203081379 2201 78-79 -0.07564575353247704 2201 80-81 -0.1571876468167872 2201 82-83 -0.1907551234305771 2201 84-85 -0.22781384956540052 2201 86-87 -0.18554809619222112 2201 88-89 -0.2512222046927022 2201 90-91 -0.1800473453425866 2201 92-93 -0.18815913793120131 2201 94-95 -0.2409479238115182 2201 96-97 -0.166580160250966 2201 98-99 -0.3371585223264262 2201 100-101 -0.38086211646894697 1101 1 0.12695489034072693 1101 2 0.11788772483275523 1101 3 0.0818631784820738 1101 4 0.01872095085259673 1101 5 0.024612676395911137 1101 6 0.1068072678785299 1101 7 0.13610683696487058 1101 8 0.10045049857136945 1101 9 0.07834998825537554 1101 10-11 0.11439573409470682 1101 12-13 0.056682021936147464 1101 14-15 0.06165252510155028 1101 16-17 0.0562999795427217 1101 18-19 0.09637842735808988 1101 20-21 0.06291409987020558 1101 22-23 0.054488070710632996 1101 24-25 0.02619699464170111 1101 26-27 -0.03393022820736746 1101 28-29 0.026972917851182387 1101 30-31 0.061414556550204225 1101 32-33 -0.0071104924191089935 1101 34-35 0.04645695222801294 1101 36-37 0.05050777326917455 1101 38-39 0.06274145147128962 1101 40-41 0.06927851613133384 1101 42-43 0.0462433740571484 1101 44-45 0.03575337047166727 1101 46-47 -0.06234826682075578 1101 48-49 -0.1183053960392968 1101 50-51 -0.06620913868025724 1101 52-53 -0.03465308767220421 1101 54-55 -0.06584780966379356 1101 56-57 -0.0501619998349625 1101 58-59 -0.027810652026246885 1101 60-61 -0.0587685877199533 1101 62-63 -0.06249833195010268 1101 64-65 -0.07013357279670629 1101 66-67 -0.05574503332355363 1101 68-69 -0.10466736884065853 1101 70-71 -0.061310064944954945 1101 72-73 -0.17312907814657308 1101 74-75 -0.16942154669462894 1101 76-77 -0.13503679467792296 1101 78-79 -0.09369721914258378 1101 80-81 -0.18651962730397287 1101 82-83 -0.24766456309833984 1101 84-85 -0.22251571614752663 1101 86-87 -0.23502435549026757 1101 88-89 -0.20466179189583045 1101 90-91 -0.31583071337097124 1101 92-93 -0.25713506012266407 1101 94-95 -0.26850859295404916 1101 96-97 -0.3426557974889093 1101 98-99 -0.3561551671313836 1101 100-101 -0.3609603263887209 2202 1 0.07132623532370985 2202 2 0.04663308518413345 2202 3 0.03264237757186805 2202 4 0.005670184469991568 2202 5 0.02995405567285303 2202 6 0.03917651549427603 2202 7 0.04275608203581527 2202 8 -0.01828069744441052 2202 9 0.0032821394864157583 2202 10-11 0.025185994401802247 2202 12-13 0.03570903590738084 2202 14-15 -0.01643329970315932 2202 16-17 -0.012509772825517018 2202 18-19 -0.04022381922167284 2202 20-21 -0.03430127615641965 2202 22-23 -0.060410370190240315 2202 24-25 -0.047060368352212834 2202 26-27 -0.02776233154573049 2202 28-29 -0.05462083251237004 2202 30-31 -0.043952565222632245 2202 32-33 -0.05851436747641259 2202 34-35 -0.1267171522765409 2202 36-37 -0.10945452396516941 2202 38-39 -0.09575838626604849 2202 40-41 -0.11226963177920624 2202 42-43 -0.12757783405032797 2202 44-45 -0.15961469854323695 2202 46-47 -0.17118105819955076 2202 48-49 -0.21905831719318059 2202 50-51 -0.22830796815483012 2202 52-53 -0.2325977938521646 2202 54-55 -0.22192436512556668 2202 56-57 -0.3044087279678749 2202 58-59 -0.26043931789809704 2202 60-61 -0.22176405044989878 2202 62-63 -0.24594461229938958 2202 64-65 -0.33340460460082255 2202 66-67 -0.19688293390509415 2202 68-69 -0.2645181533892753 2202 70-71 -0.27038049920774654 2202 72-73 -0.26109759485748896 2202 74-75 -0.3038369175031548 2202 76-77 -0.3241990700468378 2202 78-79 -0.2769704014685246 2202 80-81 -0.32375498303158423 2202 82-83 -0.3385128660771741 2202 84-85 -0.3827442731011388 2202 86-87 -0.33514765759171183 2202 88-89 -0.32895931284260627 2202 90-91 -0.368110407669775 2202 92-93 -0.3729429191263094 2202 94-95 -0.4228685325778301 2202 96-97 -0.5007508693427667 2202 98-99 -0.5011480216843154 2202 100-101 -0.5732900108281171 1102 1 0.11712017282192733 1102 2 0.03680106303460917 1102 3 0.009487713306363332 1102 4 -0.011275671910318863 1102 5 0.01394508445969933 1102 6 0.013854546610055252 1102 7 0.020080262596003706 1102 8 0.008863461283247887 1102 9 -1.5938805726278815E-4 1102 10-11 0.020970311931527874 1102 12-13 -0.031148777753266188 1102 14-15 -0.02582037875939136 1102 16-17 -0.0403255078118292 1102 18-19 -0.0700825372349172 1102 20-21 -0.023107566104194177 1102 22-23 -0.055364904980919505 1102 24-25 -0.03342689578797575 1102 26-27 -0.06832127994012183 1102 28-29 -0.08951786915638138 1102 30-31 -0.07193870640424649 1102 32-33 -0.07255736064812623 1102 34-35 -0.1272671813559043 1102 36-37 -0.14123943499298264 1102 38-39 -0.08341579272668298 1102 40-41 -0.1809077592204673 1102 42-43 -0.23031784863059812 1102 44-45 -0.25058741193900147 1102 46-47 -0.2573122702191881 1102 48-49 -0.25169363978763926 1102 50-51 -0.27701175662734556 1102 52-53 -0.23752516245244237 1102 54-55 -0.29487426162795316 1102 56-57 -0.2942894229854929 1102 58-59 -0.3076717654090899 1102 60-61 -0.2926091039113814 1102 62-63 -0.29229451029654285 1102 64-65 -0.37345736829560394 1102 66-67 -0.3384561280966736 1102 68-69 -0.3204171484316305 1102 70-71 -0.4214358195440866 1102 72-73 -0.40605690053075705 1102 74-75 -0.3955912452172612 1102 76-77 -0.3893252388487056 1102 78-79 -0.40540961359293703 1102 80-81 -0.420399755356776 1102 82-83 -0.4794687522882626 1102 84-85 -0.4587556916685429 1102 86-87 -0.4865916459261683 1102 88-89 -0.5018113320188604 1102 90-91 -0.4782951887867455 1102 92-93 -0.5469712170075027 1102 94-95 -0.670476872314488 1102 96-97 -0.7958012053424071 1102 98-99 -0.7938475303717141 1102 100-101 -0.8361538486165472 2203 1 0.11773180220603763 2203 2 0.040216704651925284 2203 3 0.04544236785977773 2203 4 0.05790979250193118 2203 5 0.0030587370467927144 2203 6 -0.045260232110841514 2203 7 0.02214636692886529 2203 8 -0.040441771410051786 2203 9 -0.001802529741098624 2203 10-11 -0.07460146034124904 2203 12-13 -0.030686017107747432 2203 14-15 -0.0320461306709916 2203 16-17 -0.0758758788660927 2203 18-19 -0.10183146865836079 2203 20-21 -0.06481167904610885 2203 22-23 0.00900527581789845 2203 24-25 -0.041509696234136584 2203 26-27 -0.10655443651329222 2203 28-29 -0.07612858415253498 2203 30-31 -0.07430066353079923 2203 32-33 -0.12103196987825271 2203 34-35 -0.1304574308897415 2203 36-37 -0.14915405696111605 2203 38-39 -0.15108933713237604 2203 40-41 -0.1430715363532613 2203 42-43 -0.12493802768526052 2203 44-45 -0.1324811690062404 2203 46-47 -0.1550395223884209 2203 48-49 -0.24921935977722853 2203 50-51 -0.20893166379586603 2203 52-53 -0.18313806971335111 2203 54-55 -0.3090088680928389 2203 56-57 -0.23577422867913356 2203 58-59 -0.17194662042933118 2203 60-61 -0.16934559233805402 2203 62-63 -0.32177476225482593 2203 64-65 -0.3039075057657854 2203 66-67 -0.3484810098390483 2203 68-69 -0.33637466657027915 2203 70-71 -0.4206103069268323 2203 72-73 -0.3867247527152884 2203 74-75 -0.3675105951051947 2203 76-77 -0.4142364347126346 2203 78-79 -0.34172333908091446 2203 80-81 -0.4526120331419108 2203 82-83 -0.4345059885559195 2203 84-85 -0.5693294829852533 2203 86-87 -0.5440434725309231 2203 88-89 -0.5461397675038597 2203 90-91 -0.607215780917258 2203 92-93 -0.6730982240696406 2203 94-95 -0.6415551455950848 2203 96-97 -0.6288521242892671 2203 98-99 -0.6164791798461735 2203 100-101 -0.6701840165040096 1103 1 0.06932703429031761 1103 2 0.060480114714664523 1103 3 0.07984872016572098 1103 4 0.08954414903855934 1103 5 0.05518616720480907 1103 6 0.061272967234572207 1103 7 0.02894904842268886 1103 8 0.018702091993404224 1103 9 -0.0016448532993038611 1103 10-11 0.01747826727017099 1103 12-13 -0.006169405275507245 1103 14-15 0.04761288213355641 1103 16-17 0.04782704276533423 1103 18-19 0.059207362968237476 1103 20-21 0.057075693276466666 1103 22-23 0.0021141227907008897 1103 24-25 0.04110753841667503 1103 26-27 0.053118903384699934 1103 28-29 0.023646091995040308 1103 30-31 0.05607412190569505 1103 32-33 -0.009623735074846707 1103 34-35 -0.02032622975471554 1103 36-37 -0.04693029637299162 1103 38-39 0.05943578025458862 1103 40-41 0.03501160260947245 1103 42-43 -0.030990441204586716 1103 44-45 -1.423667268909412E-4 1103 46-47 -0.051119322000431566 1103 48-49 -0.05967410817437013 1103 50-51 -0.08621892542830523 1103 52-53 -0.0234820360949044 1103 54-55 -0.07515724368597176 1103 56-57 -0.049554383069200014 1103 58-59 -0.042182209755111444 1103 60-61 -0.04528212209338989 1103 62-63 -0.14606506265168662 1103 64-65 -0.15769300145710474 1103 66-67 -0.09204854395384388 1103 68-69 -0.16332636153072144 1103 70-71 -0.1609785169202098 1103 72-73 -0.19793415325460018 1103 74-75 -0.23877916520122966 1103 76-77 -0.22131826400295296 1103 78-79 -0.1878553633723783 1103 80-81 -0.16443098523314603 1103 82-83 -0.2842207966979302 1103 84-85 -0.22198436192657311 1103 86-87 -0.22722379922120695 1103 88-89 -0.2670212077808145 1103 90-91 -0.2812893466039412 1103 92-93 -0.2332969149792703 1103 94-95 -0.3326551464654486 1103 96-97 -0.34736610055499995 1103 98-99 -0.24997525957302713 1103 100-101 -0.2475499236911034 2204 1 0.11235032518253263 2204 2 0.11538360804443926 2204 3 0.06713889984837351 2204 4 0.11079041647151655 2204 5 0.08819051554328183 2204 6 0.07394493403857183 2204 7 0.12050957152784036 2204 8 0.12470783682672248 2204 9 0.12166008301965547 2204 10-11 0.0646399315147903 2204 12-13 0.15903746362285887 2204 14-15 0.1419177722163596 2204 16-17 0.11067237645224992 2204 18-19 0.10013207274636216 2204 20-21 0.10021012843664323 2204 22-23 0.08957109403877439 2204 24-25 0.08649153135235821 2204 26-27 0.035100686239147194 2204 28-29 0.0765117634735688 2204 30-31 0.07991263309346408 2204 32-33 0.06061998583668071 2204 34-35 0.06382046576521105 2204 36-37 0.03904469751286399 2204 38-39 0.08267329791205924 2204 40-41 0.09425355700106053 2204 42-43 0.05832418038206555 2204 44-45 0.0667198132937159 2204 46-47 0.06459720364597388 2204 48-49 0.050988612486470686 2204 50-51 0.07362590879946396 2204 52-53 0.09740867924087127 2204 54-55 0.08390331279685626 2204 56-57 0.0870374719078697 2204 58-59 0.047061579814211996 2204 60-61 0.017466828242362453 2204 62-63 0.04532951989141765 2204 64-65 0.06684139778088394 2204 66-67 0.10911033834206307 2204 68-69 0.08630188928093219 2204 70-71 0.1076054773335926 2204 72-73 0.0893916955295424 2204 74-75 0.03998441335594549 2204 76-77 0.08273964451748128 2204 78-79 0.11067529522168229 2204 80-81 0.09503356514270678 2204 82-83 0.02014392316858249 2204 84-85 0.009669640247167877 2204 86-87 0.01881338296767865 2204 88-89 -0.06518452507825145 2204 90-91 -0.04707685204670753 2204 92-93 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1215 3 -0.10139294868098148 1215 4 -0.11481373900703318 1215 5 -0.07262641868005915 1215 6 -0.09112472547473516 1215 7 -0.13819854888497218 1215 8 -0.12589622211304174 1215 9 -0.06870528575399248 1215 10-11 -0.08640990657254832 1215 12-13 -0.1632138251425701 1215 14-15 -0.08985901855007228 1215 16-17 -0.09022948889895588 1215 18-19 -0.07908204592941104 1215 20-21 -0.06680140878373919 1215 22-23 -0.0807687342689789 1215 24-25 -0.026843943727065778 1215 26-27 -0.04217096865009751 1215 28-29 -0.02899449126761766 1215 30-31 -0.03826473801760244 1215 32-33 -0.06813588307115026 1215 34-35 -0.07806214493171382 1215 36-37 -0.06269325809578419 1215 38-39 -0.016231576354591937 1215 40-41 -0.014150586733997272 1215 42-43 0.006685843216537535 1215 44-45 -0.004912610494528735 1215 46-47 0.0336872435516824 1215 48-49 0.05543707872614334 1215 50-51 0.06680325594700065 1215 52-53 0.04610304828540279 1215 54-55 0.11821796997831058 1215 56-57 0.028327952709638282 1215 58-59 0.016554836901711667 1215 60-61 0.10408009654424433 1215 62-63 0.1080627723220502 1215 64-65 0.1281312549831739 1215 66-67 0.22448844065022655 1215 68-69 0.22076980076025876 1215 70-71 0.16692812395554313 1215 72-73 0.14756825557786613 1215 74-75 0.17882907508005985 1215 76-77 0.23080711874867177 1215 78-79 0.23767456900351647 1215 80-81 0.2621375160001165 1215 82-83 0.2380474739911591 1215 84-85 0.2368654978102569 1215 86-87 0.3051063629601032 1215 88-89 0.3106678199830881 1215 90-91 0.3235440629761257 1215 92-93 0.33516244835509923 1215 94-95 0.3457149713955161 1215 96-97 0.35313257854090807 1215 98-99 0.41137849372845636 1215 100-101 0.5024556662349369 1212 1 -0.23158674238871413 1212 2 -0.16687874470977704 1212 3 -0.10386370579867332 1212 4 -0.14954010001820706 1212 5 -0.12243913868247347 1212 6 -0.16998441784471652 1212 7 -0.2817717272563769 1212 8 -0.35142913321261204 1212 9 -0.35285724160705456 1212 10-11 -0.38489826576515185 1212 12-13 -0.2853838708682517 1212 14-15 -0.2692227211311575 1212 16-17 -0.15147979151814184 1212 18-19 -0.11707519870404326 1212 20-21 -0.11726261487472556 1212 22-23 -0.10289151584014178 1212 24-25 -0.09890482810858714 1212 26-27 -0.10935608551700682 1212 28-29 -0.036716903879735696 1212 30-31 -0.054031466490343405 1212 32-33 -0.1474452839073379 1212 34-35 -0.11536132352365058 1212 36-37 -0.24165187495840712 1212 38-39 -0.24631387376283698 1212 40-41 -0.3150996241394921 1212 42-43 -0.3721453756172721 1212 44-45 -0.10974408907786426 1212 46-47 -0.19369422662352065 1212 48-49 -0.3566705586674459 1212 50-51 -0.22061490076956858 1212 52-53 -0.3316060179412261 1212 54-55 -0.4032778275412525 1212 56-57 -0.34927325607338133 1212 58-59 -0.33905638037099806 1212 60-61 -0.3631629195560535 1212 62-63 -0.30998472577284275 1212 64-65 -0.1854708070257658 1212 66-67 -0.2016907228800946 1212 68-69 -0.3088404644858258 1212 70-71 -0.22275850382293072 1212 72-73 -0.22330067607010307 1212 74-75 -0.14541451529657934 1212 76-77 -0.2603631389392618 1212 78-79 -0.028256362443443095 1212 80-81 0.017543833254883623 1212 82-83 0.052657468487446124 1212 84-85 0.09389635360857795 1212 86-87 0.05543119830457499 1212 88-89 0.010621798118691572 1212 90-91 0.14114229171909187 1212 92-93 0.12204833864114306 1212 94-95 0.06527670409654007 1212 96-97 0.13241066280003366 1212 98-99 0.17365899092052217 1212 100-101 0.17875314417761956 1213 1 -0.39374473517731445 1213 2 -0.2399098297875284 1213 3 -0.15269998333864976 1213 4 -0.21564681477664038 1213 5 -0.23711550800815928 1213 6 -0.152881883115775 1213 7 -0.20929249564161267 1213 8 -0.20629539176241707 1213 9 -0.23210749779679674 1213 10-11 -0.2178123711451363 1213 12-13 -0.26897827162885335 1213 14-15 -0.36758540343861057 1213 16-17 -0.4763827835610144 1213 18-19 -0.4920375050805674 1213 20-21 -0.30699101277752305 1213 22-23 -0.4153502013534691 1213 24-25 -0.48571803787297796 1213 26-27 -0.2971783026872785 1213 28-29 -0.27140224692961823 1213 30-31 -0.286695170036559 1213 32-33 -0.3040775591376601 1213 34-35 -0.2455265778604101 1213 36-37 -0.1813638613808024 1213 38-39 -0.15760646930762334 1213 40-41 -0.1455504964863934 1213 42-43 -0.10357071523400663 1213 44-45 -0.15891700160592848 1213 46-47 -0.0665121232616741 1213 48-49 -0.06980554403182992 1213 50-51 -0.02579960849469387 1213 52-53 -0.049164152978335096 1213 54-55 -0.10099434602637558 1213 56-57 -0.00903475976907231 1213 58-59 -0.025773367336746844 1213 60-61 0.011118478573209245 1213 62-63 0.028691094581837717 1213 64-65 0.03797505668279655 1213 66-67 0.027566192799064027 1213 68-69 0.0029446953483756033 1213 70-71 0.016930769613793473 1213 72-73 -0.06663116255900547 1213 74-75 -0.04292982892251729 1213 76-77 0.03530251223754277 1213 78-79 0.03732261697327033 1213 80-81 0.016998487226928205 1213 82-83 0.1810604495255177 1213 84-85 0.08161236736067679 1213 86-87 0.09700984253437639 1213 88-89 0.08138309696760615 1213 90-91 0.12990720034983383 1213 92-93 0.1776391614528734 1213 94-95 0.1779004145523615 1213 96-97 0.1984655806181408 1213 98-99 0.16775021529802103 1213 100-101 0.043230312698909756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 16.0 15 61.0 16 116.0 17 233.0 18 654.0 19 1535.0 20 3386.0 21 7096.0 22 12815.0 23 20617.0 24 30634.0 25 42609.0 26 57405.0 27 74243.0 28 93898.0 29 116636.0 30 141507.0 31 170223.0 32 204754.0 33 248343.0 34 307538.0 35 394739.0 36 547345.0 37 871646.0 38 1889926.0 39 5337214.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.20508408177698 29.58377506739235 30.24582482637379 20.965316024456886 2 19.823739514929525 30.724468097480102 29.516184382345507 19.935608005244866 3 19.83773341188196 29.29504812679489 30.294169655580657 20.5730488057425 4 20.223315136654755 28.76902448088403 30.20671969014268 20.80094069231853 5 20.628035997461986 29.180723939711722 29.9037842346095 20.2874558282168 6 20.70556651937223 29.458023922028826 28.970562231033202 20.865847327565746 7 20.57045783574574 29.778708467649277 29.60817725260728 20.042656443997696 8 19.991432778039922 29.56610708649206 30.037663625901757 20.40479650956626 9 20.012693870167542 29.006902291903604 30.29841658532056 20.681987252608298 10-11 20.282492537717083 29.195038912805472 29.71092601526858 20.81154253420887 12-13 19.896886013395505 29.389041709888904 30.16823811203392 20.545834164681676 14-15 20.030099191161067 28.957535365131182 30.17833510716513 20.83403033654262 16-17 20.137113375740768 29.10987414883326 30.060230596329713 20.692781879096263 18-19 20.042651715950257 28.878507147389314 30.23732434121751 20.841516795442917 20-21 20.23749674532619 28.808549909308844 29.894356845959024 21.059596499405945 22-23 20.086652927258474 28.921120134496096 30.048325907373748 20.943901030871682 24-25 20.305229496581966 28.678007868913536 29.92041489689397 21.096347737610525 26-27 20.380632463730457 28.866411783879748 29.649490305347765 21.103465447042037 28-29 20.334436278554204 28.91115845749308 29.770249112554787 20.984156151397933 30-31 20.651441654833494 28.67659713779713 29.569442122360424 21.102519085008954 32-33 20.630887289301587 28.850308066632174 29.661623407059167 20.85718123700707 34-35 20.663347291805355 28.583906606611336 29.677841151444152 21.07490495013916 36-37 20.656072458377768 28.787572733049082 29.48409076702473 21.072264041548422 38-39 20.564298118697337 28.67861868395536 29.667094855183983 21.089988342163323 40-41 20.724953101433684 28.745771184930724 29.345087386999896 21.1841883266357 42-43 20.619078413900603 28.736376486289622 29.496442816187233 21.148102283622542 44-45 20.603961636286954 28.49146583247471 29.539861383822924 21.364711147415417 46-47 20.728788837784666 28.752403071486516 29.296729647571585 21.222078443157237 48-49 20.561883998264328 28.5609359613552 29.51009360445827 21.3670864359222 50-51 20.684334335295233 28.644758180027612 29.271957705184114 21.398949779493037 52-53 20.63027793041289 28.65940449292619 29.52139355805215 21.188924018608766 54-55 20.80167915006316 28.400792192175405 29.438174097381413 21.359354560380016 56-57 20.835641550567775 28.662802054903246 29.235646600864328 21.26590979366465 58-59 20.553328246920668 28.57135088705256 29.547351042151103 21.327969823875673 60-61 20.718238849362265 28.498706584134304 29.1777824210152 21.605272145488232 62-63 20.635162674423206 28.573166527633546 29.429677681938465 21.36199311600478 64-65 20.769706194293942 28.394041050190104 29.31987171437347 21.51638104114248 66-67 20.797843420565567 28.58220949627441 29.12101865413409 21.498928429025934 68-69 20.58362138445915 28.4853965573442 29.39801893198747 21.532963126209186 70-71 20.858521100701928 28.438270320139225 29.112991209337192 21.59021736982166 72-73 20.719274797103516 28.508639438771283 29.293805230724878 21.47828053340032 74-75 20.75886611782262 28.413231402676047 29.256159056614813 21.571743422886524 76-77 20.79962397434111 28.629526849198783 29.112660038164446 21.45818913829566 78-79 20.739030114840148 28.45730071714026 29.351045736342712 21.452623431676876 80-81 20.907221812721343 28.443713117433706 29.03329034874547 21.615774721099477 82-83 20.752410567910424 28.687334255942048 29.28375392180042 21.276501254347107 84-85 20.785183765910258 28.44962301858257 29.150743957156784 21.61444925835039 86-87 20.687979542786422 28.69113233548491 29.140539593244725 21.480348528483944 88-89 20.686203091958614 28.56663867120508 29.205950862338074 21.54120737449823 90-91 20.80741158060652 28.38651732357908 29.083602513869998 21.722468581944405 92-93 20.604236753077306 28.488340861967803 29.296518430558706 21.610903954396186 94-95 20.7516627916742 28.330567373042616 29.164738943031182 21.753030892252006 96-97 20.764680365627143 28.541756447352608 29.026106403033218 21.66745678398703 98-99 20.65338177471448 28.51646575882027 29.20667742073524 21.623475045730007 100-101 20.972661018760267 28.37680058931386 28.891807194508562 21.75873119741731 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 174.0 1 162.0 2 156.0 3 210.5 4 256.0 5 300.0 6 423.5 7 587.0 8 758.0 9 1012.5 10 1411.5 11 1991.0 12 2874.5 13 4186.0 14 6212.0 15 9175.5 16 12859.0 17 17040.5 18 22463.5 19 28992.0 20 35830.0 21 43105.5 22 51036.0 23 58916.5 24 65714.5 25 73580.5 26 81659.0 27 88164.0 28 96667.5 29 108875.0 30 124004.5 31 145642.0 32 178958.5 33 221561.5 34 271569.5 35 334647.5 36 415477.5 37 497910.0 38 561132.5 39 599634.5 40 614580.5 41 592507.5 42 535582.0 43 469831.5 44 404850.5 45 353598.0 46 318669.0 47 296888.5 48 282524.5 49 270323.0 50 259945.5 51 244640.5 52 222600.0 53 200416.0 54 183335.0 55 170372.0 56 158340.5 57 144444.5 58 130225.5 59 114835.0 60 97758.0 61 79391.5 62 63204.0 63 49937.0 64 38347.5 65 29519.0 66 22248.5 67 16544.5 68 12491.0 69 9000.5 70 6313.0 71 4654.5 72 3462.0 73 2620.0 74 1930.5 75 1226.5 76 834.5 77 587.0 78 432.5 79 318.0 80 198.0 81 124.5 82 82.5 83 53.0 84 45.5 85 38.0 86 21.0 87 12.5 88 12.5 89 9.0 90 5.0 91 4.5 92 2.5 93 2.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09286830780345318 2 0.002581513901877886 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 6.619266415071502E-5 10-11 7.56487590293886E-5 12-13 0.0 14-15 0.001465694706194404 16-17 0.0 18-19 0.0 20-21 1.2765728086209325E-4 22-23 1.2765728086209325E-4 24-25 3.0732308355689114E-4 26-27 0.0012576606188635854 28-29 0.0016169922242531814 30-31 0.0037635257617120825 32-33 0.004241058553085098 34-35 0.00575403373367287 36-37 0.0069313175460677294 38-39 0.011224384620985534 40-41 0.012349659911547689 42-43 0.01301158655305484 44-45 0.015172304232831751 46-47 0.014018660657633575 48-49 0.016661639176222837 50-51 0.015139207900756391 52-53 0.015886239396171606 54-55 0.016505613610724727 56-57 0.014690043394019399 58-59 0.011985600258718756 60-61 0.006349767711029305 62-63 0.003512939247427233 64-65 3.593316053895958E-4 66-67 0.0027942760366480414 68-69 0.0036784209078040207 70-71 0.004226874410767087 72-73 0.01719118048942856 74-75 0.012004512448476104 76-77 0.033767714811743337 78-79 0.0256402012635234 80-81 0.019106039702359957 82-83 0.03172992636538918 84-85 0.01129057728513625 86-87 0.01614155395789579 88-89 0.008103873311023253 90-91 0.006217382382727875 92-93 0.012652254947665241 94-95 0.013295269399415046 96-97 0.007668892946604269 98-99 0.008486845153609533 100-101 0.0017020970781612436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.057519E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.988397689342504 #Duplication Level Percentage of deduplicated Percentage of total 1 28.68066089755098 4.5855781242322 2 5.909374350039598 1.8896285440726592 3 6.196831078362516 2.972321990836112 4 6.905998080660866 4.416633750217678 5 7.286164959589332 5.82470515020332 6 7.281147011792037 6.984832443545932 7 6.165452142651455 6.900299055392423 8 5.815707382335802 7.43870739789039 9 4.980907922837527 7.167306305189094 >10 20.34889496616589 46.50626755074514 >50 0.3680865246603311 3.8619427811895792 >100 0.06011207434261294 1.312477711756925 >500 0.0 0.0 >1k 6.626090110671784E-4 0.13929919472854038 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.891218975734715E-5 0.0 1.2292923342275647E-4 2 2.8368284636020723E-5 3.78243795146943E-5 1.2292923342275647E-4 3 2.8368284636020723E-5 3.78243795146943E-5 1.2292923342275647E-4 4 2.8368284636020723E-5 3.78243795146943E-5 1.2292923342275647E-4 5 2.8368284636020723E-5 3.78243795146943E-5 1.2292923342275647E-4 6 2.8368284636020723E-5 3.78243795146943E-5 1.4184142318010362E-4 7 3.78243795146943E-5 3.78243795146943E-5 1.4184142318010362E-4 8 3.78243795146943E-5 3.78243795146943E-5 1.4184142318010362E-4 9 3.78243795146943E-5 3.78243795146943E-5 1.4184142318010362E-4 10-11 4.2552426954031085E-5 3.78243795146943E-5 1.4184142318010362E-4 12-13 8.510485390806217E-5 3.78243795146943E-5 1.4184142318010362E-4 14-15 1.3711337574076683E-4 3.78243795146943E-5 1.4184142318010362E-4 16-17 1.512975180587772E-4 3.78243795146943E-5 1.4184142318010362E-4 18-19 2.22218229648829E-4 3.78243795146943E-5 1.4184142318010362E-4 20-21 2.9786698867821757E-4 3.78243795146943E-5 1.4184142318010362E-4 22-23 4.2552426954031085E-4 3.78243795146943E-5 1.4184142318010362E-4 24-25 6.241022619924559E-4 3.78243795146943E-5 1.4184142318010362E-4 26-27 8.746887762773057E-4 7.56487590293886E-5 1.4184142318010362E-4 28-29 0.0010921789584867978 1.0401704366540932E-4 1.4184142318010362E-4 30-31 0.0012860289034996061 1.0401704366540932E-4 1.4184142318010362E-4 32-33 0.0015697117498598135 1.0401704366540932E-4 1.4184142318010362E-4 34-35 0.0018581226436593574 1.0401704366540932E-4 1.9857799245214506E-4 36-37 0.0020756128258688496 1.891218975734715E-4 1.9857799245214506E-4 38-39 0.002359295672229057 1.891218975734715E-4 1.9857799245214506E-4 40-41 0.0027422675148153367 1.891218975734715E-4 1.9857799245214506E-4 42-43 0.0033238173498537616 1.891218975734715E-4 1.9857799245214506E-4 44-45 0.0037824379514694298 1.891218975734715E-4 1.9857799245214506E-4 46-47 0.004307251217235813 1.891218975734715E-4 1.9857799245214506E-4 48-49 0.00470913524957944 1.891218975734715E-4 1.9857799245214506E-4 50-51 0.005021186380575669 1.891218975734715E-4 1.9857799245214506E-4 52-53 0.005498719171948684 2.174901822094922E-4 1.9857799245214506E-4 54-55 0.006160645813455834 2.4585846684551293E-4 1.9857799245214506E-4 56-57 0.006586170082996145 2.4585846684551293E-4 1.9857799245214506E-4 58-59 0.00719608820267059 2.553145617241865E-4 1.9857799245214506E-4 60-61 0.007820190464663045 2.553145617241865E-4 1.9857799245214506E-4 62-63 0.008349731777868765 2.553145617241865E-4 1.9857799245214506E-4 64-65 0.008969105992421886 2.553145617241865E-4 1.9857799245214506E-4 66-67 0.009527015590263626 2.553145617241865E-4 2.4585846684551293E-4 68-69 0.010188942231770777 2.553145617241865E-4 2.931389412388808E-4 70-71 0.01100689443877604 2.553145617241865E-4 2.931389412388808E-4 72-73 0.011612084511011148 2.553145617241865E-4 3.78243795146943E-4 74-75 0.012496229382167129 2.553145617241865E-4 3.78243795146943E-4 76-77 0.013129787739038258 2.553145617241865E-4 3.78243795146943E-4 78-79 0.01380589852286342 2.553145617241865E-4 3.78243795146943E-4 80-81 0.014623850729868684 2.553145617241865E-4 3.78243795146943E-4 82-83 0.015682933356280123 2.553145617241865E-4 3.78243795146943E-4 84-85 0.0165150697056034 2.553145617241865E-4 3.78243795146943E-4 86-87 0.01735193410236601 2.553145617241865E-4 3.78243795146943E-4 88-89 0.018798716618803064 2.647706566028601E-4 3.78243795146943E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 690 6.706614E-9 11.012491 3 TGCCGTC 1060 6.853588E-8 8.064571 5 GCCGTCT 1115 1.3090394E-6 7.2408366 6 CGTATGC 1155 2.247898E-6 6.9965224 1 ATGCCGT 1215 5.039341E-6 6.6448827 4 GTTACGT 865 0.008561166 6.0393934 2 >>END_MODULE