FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n01 8-59796.34100000001b89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n01 8-59796.34100000001b89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10575190
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6906.706614E-911.0124913
TGCCGTC10606.853588E-88.0645715
GCCGTCT11151.3090394E-67.24083666
CGTATGC11552.247898E-66.99652241
ATGCCGT12155.039341E-66.64488274
GTTACGT8650.0085611666.03939342