##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n01 7-9252.34100000001b6f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9407964 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.766269832665174 38.0 38.0 38.0 32.0 38.0 2 36.758829115417534 38.0 38.0 38.0 32.0 38.0 3 36.74776465981375 38.0 38.0 38.0 32.0 38.0 4 36.747008704540114 38.0 38.0 38.0 32.0 38.0 5 36.73621603994233 38.0 38.0 38.0 32.0 38.0 6 38.6845557657321 40.0 38.0 40.0 38.0 40.0 7 38.63361775193868 40.0 38.0 40.0 38.0 40.0 8 38.626604225951546 40.0 38.0 40.0 38.0 40.0 9 38.626495381997636 40.0 38.0 40.0 38.0 40.0 10-11 38.640686922271385 40.0 38.0 40.0 38.0 40.0 12-13 38.63360148912135 40.0 38.0 40.0 38.0 40.0 14-15 38.619913671013194 40.0 38.0 40.0 38.0 40.0 16-17 38.60296016226253 40.0 38.0 40.0 38.0 40.0 18-19 38.573153181708605 40.0 38.0 40.0 38.0 40.0 20-21 38.550737013874624 40.0 38.0 40.0 38.0 40.0 22-23 38.53041768654727 40.0 38.0 40.0 38.0 40.0 24-25 38.48615826973828 40.0 38.0 40.0 38.0 40.0 26-27 38.4109628289394 40.0 38.0 40.0 38.0 40.0 28-29 38.369001624581045 40.0 38.0 40.0 38.0 40.0 30-31 38.31763859853205 40.0 38.0 40.0 38.0 40.0 32-33 38.283314487597956 40.0 38.0 40.0 38.0 40.0 34-35 38.252001070582324 40.0 38.0 40.0 38.0 40.0 36-37 38.20843308924226 40.0 38.0 40.0 38.0 40.0 38-39 38.15855231801483 40.0 38.0 40.0 38.0 40.0 40-41 38.113616984503764 40.0 38.0 40.0 38.0 40.0 42-43 38.070294592964004 40.0 38.0 40.0 38.0 40.0 44-45 38.04870591554135 40.0 38.0 40.0 38.0 40.0 46-47 38.008462192244785 40.0 38.0 40.0 38.0 40.0 48-49 37.95952067843797 40.0 38.0 40.0 38.0 40.0 50-51 37.906223865227375 40.0 38.0 40.0 35.0 40.0 52-53 37.86164992765704 40.0 38.0 40.0 32.0 40.0 54-55 37.80913633385502 40.0 38.0 40.0 32.0 40.0 56-57 37.75197221205353 40.0 38.0 40.0 32.0 40.0 58-59 37.72673481743765 40.0 38.0 40.0 32.0 40.0 60-61 37.65934611356931 40.0 38.0 40.0 32.0 40.0 62-63 37.62376678949877 40.0 38.0 40.0 32.0 40.0 64-65 37.57950402446268 40.0 38.0 40.0 32.0 40.0 66-67 37.48776254883629 40.0 38.0 40.0 32.0 40.0 68-69 37.429646786488554 40.0 38.0 40.0 32.0 40.0 70-71 37.4017255486947 40.0 38.0 40.0 32.0 40.0 72-73 37.350034927854736 40.0 38.0 40.0 32.0 40.0 74-75 37.273462621668195 39.0 38.0 40.0 32.0 40.0 76-77 37.20135621267258 38.0 38.0 40.0 32.0 40.0 78-79 37.11313606217031 38.0 38.0 40.0 32.0 40.0 80-81 37.06635271988711 38.0 38.0 40.0 32.0 40.0 82-83 36.98286494293558 38.0 38.0 40.0 32.0 40.0 84-85 36.91185802794314 38.0 38.0 40.0 32.0 40.0 86-87 36.85045138352996 38.0 38.0 40.0 32.0 40.0 88-89 36.804699135753495 38.0 38.0 40.0 32.0 40.0 90-91 36.73453974738849 38.0 38.0 40.0 32.0 40.0 92-93 36.66226900953278 38.0 38.0 40.0 32.0 40.0 94-95 36.57637518595947 38.0 38.0 40.0 32.0 40.0 96-97 36.48657079257531 38.0 38.0 40.0 32.0 40.0 98-99 36.379592279477265 38.0 38.0 40.0 32.0 40.0 100-101 35.48780676669256 38.0 35.0 39.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.06833741078592226 2201 2 0.047291137214308776 2201 3 0.10906127223363882 2201 4 0.03921850660358217 2201 5 0.1066620791518389 2201 6 0.12521074254934916 2201 7 0.18731922376070287 2201 8 0.13105291293283017 2201 9 0.13163286555785447 2201 10-11 0.047623824356250566 2201 12-13 0.008719282763607339 2201 14-15 0.0038139090469186954 2201 16-17 0.07203656610645481 2201 18-19 0.06130001835526855 2201 20-21 0.0936664112486838 2201 22-23 0.06422363435660117 2201 24-25 0.07471910682748728 2201 26-27 0.0769182746541972 2201 28-29 0.03673823193654613 2201 30-31 0.020627977955015808 2201 32-33 -0.02882898764632813 2201 34-35 0.0025572283590094003 2201 36-37 0.026169284383001923 2201 38-39 0.014621349386253257 2201 40-41 0.004336168738852564 2201 42-43 -0.03177863382486379 2201 44-45 0.007899310532600623 2201 46-47 -0.04622655321495017 2201 48-49 -0.04533133783840526 2201 50-51 -0.05534518430731339 2201 52-53 -0.04993189228242301 2201 54-55 -0.17737812189404423 2201 56-57 -0.14874752945846126 2201 58-59 -0.05911519937536269 2201 60-61 -0.009652597598261536 2201 62-63 -0.08838048606943971 2201 64-65 -0.11486711221113666 2201 66-67 -0.05575928488429582 2201 68-69 -0.11498647713752774 2201 70-71 -0.09671937984378332 2201 72-73 -0.10890431437407955 2201 74-75 -0.08670291280628106 2201 76-77 -0.11523650753063919 2201 78-79 -0.19403237194686795 2201 80-81 -0.1278936524023777 2201 82-83 -0.20954248248146 2201 84-85 -0.2640769180298932 2201 86-87 -0.29535146059836137 2201 88-89 -0.3063389816058262 2201 90-91 -0.28001606917902677 2201 92-93 -0.3561769345468875 2201 94-95 -0.2935296742717597 2201 96-97 -0.17268963656051994 2201 98-99 -0.27958158690658763 2201 100-101 -0.3728945413375655 1101 1 0.12614566191037824 1101 2 0.03398619052909879 1101 3 0.09820654075635105 1101 4 0.05638365649919308 1101 5 0.010678677169330797 1101 6 0.0786867621311984 1101 7 0.11247991995035989 1101 8 0.08757138039295853 1101 9 0.058590142257536115 1101 10-11 0.07923450009678845 1101 12-13 0.009373388827960127 1101 14-15 0.008977188455695284 1101 16-17 -0.026045552463692445 1101 18-19 0.0064565803992451265 1101 20-21 -0.010456756177561033 1101 22-23 0.016951935805458618 1101 24-25 0.004193395289838975 1101 26-27 -0.059103651977757465 1101 28-29 -0.010057052610328299 1101 30-31 0.053403606429057504 1101 32-33 0.009341973287682492 1101 34-35 0.010406638613119412 1101 36-37 0.017276789264315084 1101 38-39 -0.06063123478377008 1101 40-41 -0.038521457923579305 1101 42-43 -0.004160045700821513 1101 44-45 -0.03328062798799181 1101 46-47 -0.08076020835103748 1101 48-49 -0.11591444478872148 1101 50-51 -0.08290960784293588 1101 52-53 -0.08990342724780476 1101 54-55 -0.13244638135422804 1101 56-57 -0.05628376514913214 1101 58-59 -0.09612589505339741 1101 60-61 -0.1306670542830517 1101 62-63 -0.08420538060659055 1101 64-65 -0.12809515414983963 1101 66-67 -0.1397554156465617 1101 68-69 -0.07537542621547288 1101 70-71 -0.13280190914433376 1101 72-73 -0.11866932516814899 1101 74-75 -0.16810825313551447 1101 76-77 -0.19543060628580378 1101 78-79 -0.18467299162774253 1101 80-81 -0.1590604187837812 1101 82-83 -0.3605348931607395 1101 84-85 -0.26227614432244195 1101 86-87 -0.22978376147177926 1101 88-89 -0.3245460968706837 1101 90-91 -0.3320675054198503 1101 92-93 -0.37888552744410475 1101 94-95 -0.3610047381188366 1101 96-97 -0.3655294701212881 1101 98-99 -0.3766620975868378 1101 100-101 -0.43464065329590795 2202 1 0.0943929081256627 2202 2 0.0848564349539771 2202 3 0.03618523196249157 2202 4 0.031262803628592906 2202 5 0.022427222719258566 2202 6 0.04445649363428572 2202 7 0.02170029826194053 2202 8 0.033562812576477086 2202 9 0.012958309602993268 2202 10-11 0.026072517619979863 2202 12-13 0.03365223794047978 2202 14-15 -0.010128691932592915 2202 16-17 -0.0010968893853018358 2202 18-19 -0.01978439496649287 2202 20-21 -0.0812971958828399 2202 22-23 -0.01537040234885012 2202 24-25 -0.027569671000350127 2202 26-27 -0.05148739305048622 2202 28-29 -0.07175774541934743 2202 30-31 -0.011748735010783662 2202 32-33 -0.03515144212073551 2202 34-35 -0.13615609753291125 2202 36-37 -0.11944609264004669 2202 38-39 -0.09912585059813495 2202 40-41 -0.09458793578193792 2202 42-43 -0.17705621927565574 2202 44-45 -0.21131144098392696 2202 46-47 -0.15460282806186854 2202 48-49 -0.19125902756044155 2202 50-51 -0.26678000494720777 2202 52-53 -0.2595704604431006 2202 54-55 -0.21121194047316294 2202 56-57 -0.30408415919798415 2202 58-59 -0.2363300527284835 2202 60-61 -0.21512354081774276 2202 62-63 -0.2717236828567309 2202 64-65 -0.35064158317452865 2202 66-67 -0.2211152866539834 2202 68-69 -0.22953542872281218 2202 70-71 -0.22276299070853867 2202 72-73 -0.24939444314955495 2202 74-75 -0.27089055132642414 2202 76-77 -0.2903532628420251 2202 78-79 -0.27190537982131247 2202 80-81 -0.3829668544635467 2202 82-83 -0.3719097351095968 2202 84-85 -0.36448732358232405 2202 86-87 -0.3964670199261917 2202 88-89 -0.41668418433766874 2202 90-91 -0.4148889198976278 2202 92-93 -0.4137354430094362 2202 94-95 -0.5230569111743506 2202 96-97 -0.6225392536324961 2202 98-99 -0.5686963394497937 2202 100-101 -0.5106359437963164 1102 1 0.04681884070871689 1102 2 0.03450093894849715 1102 3 -0.01935551841898331 1102 4 -0.10240163622471954 1102 5 -0.056593518577557234 1102 6 -0.006533620373566862 1102 7 0.0339827237805892 1102 8 0.017522197669840978 1102 9 -0.013511916131250246 1102 10-11 -0.039794390129202384 1102 12-13 -0.06721160512215363 1102 14-15 -0.06021365101111087 1102 16-17 -0.056132832813482025 1102 18-19 -0.11911594130513947 1102 20-21 -0.07856689971384867 1102 22-23 -0.08481162636189765 1102 24-25 -0.03718913897826326 1102 26-27 -0.10476652076393123 1102 28-29 -0.17416331348287173 1102 30-31 -0.10537455070803503 1102 32-33 -0.1335274055933482 1102 34-35 -0.1590889814901999 1102 36-37 -0.17275539518447403 1102 38-39 -0.12321583365100963 1102 40-41 -0.19991614555330983 1102 42-43 -0.22438581252718848 1102 44-45 -0.2619215066731968 1102 46-47 -0.28072179741180037 1102 48-49 -0.2813137700976327 1102 50-51 -0.2877047404028872 1102 52-53 -0.2427745747619099 1102 54-55 -0.2344281630829954 1102 56-57 -0.30001825667194737 1102 58-59 -0.3042964962309256 1102 60-61 -0.35292962286077056 1102 62-63 -0.3296437572987827 1102 64-65 -0.3614504478378677 1102 66-67 -0.2776888088756948 1102 68-69 -0.33013067141041574 1102 70-71 -0.4350622890566669 1102 72-73 -0.4650133918708619 1102 74-75 -0.3569961306610523 1102 76-77 -0.4307321689673813 1102 78-79 -0.4336108962751055 1102 80-81 -0.4254348364789138 1102 82-83 -0.534257801980452 1102 84-85 -0.5164707999777107 1102 86-87 -0.5524832478093984 1102 88-89 -0.5200223697474087 1102 90-91 -0.556370226836556 1102 92-93 -0.5879193336245905 1102 94-95 -0.6617811578033539 1102 96-97 -0.7366308730096875 1102 98-99 -0.8172244715257193 1102 100-101 -0.7979078885034312 2203 1 0.08307764717108768 2203 2 0.04253346590350304 2203 3 0.06007610221897153 2203 4 -0.009497296011836909 2203 5 -0.019319380203327796 2203 6 -0.10264100513686714 2203 7 -0.022953291831562694 2203 8 -0.07165931365918965 2203 9 0.04221512922956805 2203 10-11 -0.047057706040440905 2203 12-13 -0.033446569582395114 2203 14-15 -0.016699991106591483 2203 16-17 -0.010474978061090212 2203 18-19 -0.07775967526502825 2203 20-21 -0.03780183759342037 2203 22-23 -0.03606959735331117 2203 24-25 -0.03994018418147505 2203 26-27 -0.16424857369950985 2203 28-29 -0.07964505865245286 2203 30-31 -0.07831115448004766 2203 32-33 -0.10863371338265182 2203 34-35 -0.10152685883944912 2203 36-37 -0.1589232520213173 2203 38-39 -0.06018024383012488 2203 40-41 -0.09102312673598334 2203 42-43 -0.047084001757276894 2203 44-45 -0.12363588833257921 2203 46-47 -0.1042075289766018 2203 48-49 -0.24982423770311613 2203 50-51 -0.18955344105042116 2203 52-53 -0.16372182921487877 2203 54-55 -0.325810710873327 2203 56-57 -0.21810790882440756 2203 58-59 -0.19827798943200747 2203 60-61 -0.22374518911848895 2203 62-63 -0.3319205480786991 2203 64-65 -0.33014998770237725 2203 66-67 -0.27650991691827187 2203 68-69 -0.30508463344772707 2203 70-71 -0.3483902559669758 2203 72-73 -0.370250842604527 2203 74-75 -0.3290427444734547 2203 76-77 -0.3491058922045269 2203 78-79 -0.35658093958504367 2203 80-81 -0.38480598053813253 2203 82-83 -0.4808784890693758 2203 84-85 -0.5474124392475943 2203 86-87 -0.437937445754784 2203 88-89 -0.5414309006054197 2203 90-91 -0.5039517481452194 2203 92-93 -0.5829614286854934 2203 94-95 -0.5635604631602931 2203 96-97 -0.684101338109798 2203 98-99 -0.5610030773347887 2203 100-101 -0.6310840025549211 1103 1 0.028460436383035415 1103 2 0.013732344191183188 1103 3 0.07050317435195552 1103 4 0.033405410138897196 1103 5 0.03899680274756889 1103 6 -0.01697641139661954 1103 7 0.04083743502263104 1103 8 0.0495783062990256 1103 9 -0.0318321715310006 1103 10-11 0.02781136571378795 1103 12-13 -0.001336025674916641 1103 14-15 0.0013669558951505678 1103 16-17 0.047294746796907816 1103 18-19 -0.03391575287113824 1103 20-21 -0.01451149617960823 1103 22-23 -0.024868929915413673 1103 24-25 -0.010436155454627283 1103 26-27 -0.033451450894865786 1103 28-29 -0.05225045430633912 1103 30-31 -0.034552045246670104 1103 32-33 -0.0700017634379293 1103 34-35 -0.06017162230833151 1103 36-37 -0.04700190232080104 1103 38-39 -0.016071866666102608 1103 40-41 -0.05167129086906641 1103 42-43 -0.0814509558853942 1103 44-45 -0.10572883137307798 1103 46-47 -0.07448050168282805 1103 48-49 -0.13382226533646957 1103 50-51 -0.1586674013164071 1103 52-53 -0.051018002398109275 1103 54-55 -0.12508080384984765 1103 56-57 -0.037132057004647834 1103 58-59 -0.13784696466047563 1103 60-61 -0.07214245231155303 1103 62-63 -0.16349846812821056 1103 64-65 -0.2010980129467157 1103 66-67 -0.16164254363609132 1103 68-69 -0.2304306491706285 1103 70-71 -0.24112400425760683 1103 72-73 -0.22707180051717302 1103 74-75 -0.21550231845574785 1103 76-77 -0.2125865810657288 1103 78-79 -0.20054601616335077 1103 80-81 -0.19488578431055004 1103 82-83 -0.25192857957899406 1103 84-85 -0.21104647304660773 1103 86-87 -0.2426510311711283 1103 88-89 -0.2883659200413575 1103 90-91 -0.35566875108261087 1103 92-93 -0.35194155726017584 1103 94-95 -0.37433959913443715 1103 96-97 -0.4014833102880999 1103 98-99 -0.29382957196402515 1103 100-101 -0.3254883742196668 2204 1 0.12186329398067386 2204 2 0.04889819552727914 2204 3 0.06185242291035564 2204 4 0.09716062529201963 2204 5 0.09501320141347946 2204 6 0.06630830190786696 2204 7 0.11208724179292062 2204 8 0.0890343462868799 2204 9 0.1160562792035833 2204 10-11 0.09465072651181572 2204 12-13 0.1806760134991947 2204 14-15 0.10009048410767463 2204 16-17 0.10764306462878892 2204 18-19 0.12775208687997974 2204 20-21 0.09770439467123992 2204 22-23 0.07242791414409311 2204 24-25 0.07950203125535182 2204 26-27 0.038589965206121235 2204 28-29 0.054240290857912044 2204 30-31 0.08344150277978457 2204 32-33 0.054987241115270535 2204 34-35 0.0888940420245703 2204 36-37 0.1041122209911407 2204 38-39 0.07642168155160078 2204 40-41 0.09218504523768445 2204 42-43 0.12061959507596498 2204 44-45 0.13680492556385815 2204 46-47 0.0763335630031321 2204 48-49 0.08121317827933439 2204 50-51 0.1151136832584001 2204 52-53 0.1591755212905568 2204 54-55 0.0752726951884739 2204 56-57 0.08789312091558799 2204 58-59 0.14531426468101927 2204 60-61 0.1160227804162588 2204 62-63 0.1587067472079582 2204 64-65 0.10620809739615567 2204 66-67 0.12323522672062381 2204 68-69 0.10586671546843718 2204 70-71 0.13980733918354105 2204 72-73 0.13565553092599458 2204 74-75 0.08788380376859806 2204 76-77 0.051854406266109265 2204 78-79 0.08897633528911086 2204 80-81 0.06340224443055575 2204 82-83 0.04618429903035093 2204 84-85 0.07861908559667796 2204 86-87 0.0825706659422707 2204 88-89 0.06169772759901093 2204 90-91 -0.05413139391208688 2204 92-93 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-0.05587637377593779 1215 7 -0.13063384526034838 1215 8 -0.11240949035340009 1215 9 -0.04780324144981307 1215 10-11 -0.08787587953966636 1215 12-13 -0.1474300640212931 1215 14-15 -0.13949504743950314 1215 16-17 -0.051909698231263235 1215 18-19 -0.06289413990113246 1215 20-21 -0.06754272134495665 1215 22-23 -0.06328104778167187 1215 24-25 -0.05269833821100178 1215 26-27 -0.035234359530043946 1215 28-29 -0.001081319292616456 1215 30-31 -0.07839256027156694 1215 32-33 -0.038632370644627656 1215 34-35 -0.07098400641325497 1215 36-37 -0.052449465992140176 1215 38-39 -0.012524926518601376 1215 40-41 -0.02000015350015616 1215 42-43 0.01367522922171105 1215 44-45 -0.008557082946360595 1215 46-47 0.05238035299672106 1215 48-49 0.06920437411270797 1215 50-51 0.0610394467606028 1215 52-53 0.07414907572156437 1215 54-55 0.11018640315961648 1215 56-57 0.032201923374721275 1215 58-59 0.04578165020837588 1215 60-61 0.13867258082358802 1215 62-63 0.08002525404862126 1215 64-65 0.18144910751292542 1215 66-67 0.20104651141699037 1215 68-69 0.18693187395273725 1215 70-71 0.19415277487782845 1215 72-73 0.15681626348695232 1215 74-75 0.17965999586171222 1215 76-77 0.2052433511947882 1215 78-79 0.2752853007163125 1215 80-81 0.3150654201077643 1215 82-83 0.2480799462913481 1215 84-85 0.2706541375659839 1215 86-87 0.3690467968467104 1215 88-89 0.3167748013407632 1215 90-91 0.267476938900721 1215 92-93 0.3699051284468595 1215 94-95 0.3517643897707998 1215 96-97 0.35324267091046124 1215 98-99 0.43232871093014325 1215 100-101 0.5006765201196473 1212 1 -0.18514614964373521 1212 2 -0.15719586784322814 1212 3 -0.15449085235217552 1212 4 -0.13424253502770256 1212 5 -0.13464964361327958 1212 6 -0.18038641811992306 1212 7 -0.19090489244720033 1212 8 -0.27232323364520283 1212 9 -0.38326037665596147 1212 10-11 -0.36228618482295616 1212 12-13 -0.272000322872465 1212 14-15 -0.23975827834501473 1212 16-17 -0.14770550102785052 1212 18-19 -0.10947778868197133 1212 20-21 -0.08559762947132299 1212 22-23 -0.07011175174876882 1212 24-25 -0.06797492128386295 1212 26-27 -0.07821309383044905 1212 28-29 -0.05411331397210262 1212 30-31 -0.007780814261224123 1212 32-33 -0.1298731988428372 1212 34-35 -0.09802967622270842 1212 36-37 -0.17675630253977914 1212 38-39 -0.24558318066171836 1212 40-41 -0.2441131262683527 1212 42-43 -0.3551897425781192 1212 44-45 -0.07156590122220763 1212 46-47 -0.1677733982406835 1212 48-49 -0.3125554444764518 1212 50-51 -0.16261293270477495 1212 52-53 -0.3129961400262431 1212 54-55 -0.38775391445418705 1212 56-57 -0.3248464880820521 1212 58-59 -0.3148462663482974 1212 60-61 -0.36950832582711257 1212 62-63 -0.3152405693517011 1212 64-65 -0.12999789946172058 1212 66-67 -0.16295748173199343 1212 68-69 -0.3126462020419325 1212 70-71 -0.14774233107477386 1212 72-73 -0.15541039556747194 1212 74-75 -0.08093263965972852 1212 76-77 -0.2245110385877993 1212 78-79 0.02283797353795336 1212 80-81 0.040514076898872986 1212 82-83 0.03697130170488805 1212 84-85 0.10683722822329855 1212 86-87 0.03849491703363128 1212 88-89 0.024005075403763954 1212 90-91 0.10646251042124533 1212 92-93 0.14990720451188366 1212 94-95 0.0819062363961578 1212 96-97 0.12431569132469633 1212 98-99 0.14898576171424338 1212 100-101 0.21136256304832557 1213 1 -0.35246211384215087 1213 2 -0.16807779906628184 1213 3 -0.1590379738216896 1213 4 -0.1906214728705109 1213 5 -0.14936214265888736 1213 6 -0.0674614553380124 1213 7 -0.1401482753224812 1213 8 -0.20139268387119813 1213 9 -0.18625118865641355 1213 10-11 -0.1855542179501697 1213 12-13 -0.1934416551750786 1213 14-15 -0.25973259734367105 1213 16-17 -0.41167540811295567 1213 18-19 -0.3982010252201462 1213 20-21 -0.24007046324096848 1213 22-23 -0.3120229349115107 1213 24-25 -0.36762699955740175 1213 26-27 -0.251191968498496 1213 28-29 -0.22757327985809184 1213 30-31 -0.24636208538557014 1213 32-33 -0.24558792802031348 1213 34-35 -0.149804855358866 1213 36-37 -0.08438779518577633 1213 38-39 -0.107829213219226 1213 40-41 -0.1028022437103715 1213 42-43 -0.0827529566433185 1213 44-45 -0.08470514761708614 1213 46-47 -0.048521176800058186 1213 48-49 -0.029106395647396255 1213 50-51 -0.010207863751602986 1213 52-53 -0.009041271101878579 1213 54-55 -0.018157036825613204 1213 56-57 -0.010596814946708832 1213 58-59 -0.02695996455992855 1213 60-61 -0.031507164623647554 1213 62-63 0.021310362945534678 1213 64-65 0.014029351368591847 1213 66-67 0.10349951507446065 1213 68-69 0.06588608465200707 1213 70-71 0.036124560460891075 1213 72-73 -0.03209522132083009 1213 74-75 -0.0012069920468178452 1213 76-77 0.10042659490867578 1213 78-79 0.09508794387304675 1213 80-81 0.05187577721385139 1213 82-83 0.17128456906899459 1213 84-85 0.05256324381559807 1213 86-87 0.13582889808954235 1213 88-89 0.08757278112877742 1213 90-91 0.14766997019564343 1213 92-93 0.20225536825986268 1213 94-95 0.22024500062375552 1213 96-97 0.16990128843890062 1213 98-99 0.19285318119849393 1213 100-101 0.07880947859405296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 14.0 15 45.0 16 98.0 17 244.0 18 555.0 19 1437.0 20 3300.0 21 6797.0 22 12435.0 23 19391.0 24 28186.0 25 38570.0 26 51185.0 27 66716.0 28 83503.0 29 103291.0 30 124841.0 31 150868.0 32 179893.0 33 218440.0 34 272046.0 35 350148.0 36 484237.0 37 768163.0 38 1664144.0 39 4779412.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.386477950886402 29.093143732362687 29.890857728201443 21.629520588549468 2 20.13625999840557 29.999107119130503 29.49808402646754 20.366548855996385 3 20.255923598347103 28.682178205613884 29.975933156206807 21.08596503983221 4 20.511866329420478 28.446835043161308 29.844299999447276 21.196998627970938 5 21.21035964848505 28.63460149294789 29.430405983696367 20.724632874870693 6 21.131787919256496 28.904755587925298 28.635303026244575 21.32815346657364 7 21.206990162802494 29.228460057882877 29.034252256917647 20.53029752239698 8 20.50537183177997 29.008731325927695 29.47798269636236 21.00791414592998 9 20.366751290078312 28.64358634330348 29.80941875153328 21.180243615084933 10-11 20.770511518014857 28.829848092434084 29.269300521962364 21.13033986758869 12-13 20.374604962348922 28.85222031036683 29.641737574676092 21.13143715260815 14-15 20.396290069411325 28.606471566081492 29.735036694430416 21.26220167007677 16-17 20.557487252289654 28.6346386954712 29.582213537381737 21.22566051485741 18-19 20.423542224438783 28.47223373728896 29.693858309831967 21.410365728440286 20-21 20.631935319853998 28.3555360826764 29.478468570725212 21.534060026744385 22-23 20.632667052864864 28.411604015752634 29.50893127620789 21.446797655174613 24-25 20.71018644042935 28.203220514420906 29.42040540658325 21.666187638566495 26-27 20.829552357633442 28.337297223753154 29.210031627859518 21.623118790753885 28-29 20.78090237487296 28.42866114887436 29.26877178675565 21.521664689497026 30-31 21.07378521043108 28.13428203975566 29.175427741159105 21.61650500865416 32-33 21.10976030668091 28.264089222681793 29.13933183333452 21.486818637302775 34-35 21.141058684358942 28.21256068945141 29.024090182416085 21.62229044377357 36-37 21.17755591379921 28.193595718140845 29.003646151763483 21.625202216296454 38-39 21.10498251869982 28.115813600407957 29.13344330215164 21.645760578740582 40-41 21.238803477516914 28.259289769015634 28.773214389931905 21.728692363535547 42-43 21.120877387262567 28.190164995401705 29.05282240502482 21.636135212310908 44-45 21.096811861176793 27.943235110328395 28.99093546903574 21.969017559459076 46-47 21.211641216220958 28.265675140746232 28.780842821128804 21.741840821904002 48-49 21.003533928032 28.00699758467344 29.03589937838523 21.95356910890933 50-51 21.123190456865043 28.105036356305884 28.75648650236099 22.015286684468087 52-53 21.108790308370278 28.09023685033728 28.949959163734952 21.85101367755749 54-55 21.256964267660486 27.90518145660049 28.805599458837055 22.03225481690197 56-57 21.289649886672276 28.083391940429713 28.7544389859065 21.872519186991514 58-59 21.14869698529004 28.070465820231057 28.91810799535254 21.86272919912636 60-61 21.281781799391947 27.94572968121123 28.647119833143925 22.1253686862529 62-63 21.20981151999029 28.030033625274648 28.849823423062208 21.910331431672855 64-65 21.34259226288639 27.837816981360508 28.784810936564455 22.03477981918865 66-67 21.288186932156034 28.015774042931064 28.596795997846026 22.09924302706688 68-69 21.059668144705395 28.001160122834413 28.810296371841797 22.128875360618398 70-71 21.4551959180055 27.898881941519786 28.56853500132553 22.07738713914918 72-73 21.33327450783925 27.968226791415695 28.67376063144561 22.024738069299442 74-75 21.283608307414212 27.931621144050077 28.702247708206784 22.082522840328927 76-77 21.32715033014851 28.076206946160042 28.561751046576994 22.034891677114448 78-79 21.333541791548445 27.923222450715862 28.709767688523023 22.033468069212663 80-81 21.517269643949227 27.940323229241226 28.41377297726573 22.128634149543824 82-83 21.355835608281787 28.22744877046457 28.597455070321825 21.819260550931823 84-85 21.314032486132085 27.94252990804439 28.608037812342683 22.135399793480843 86-87 21.254813365303455 28.183412293883674 28.454363505709168 22.107410835103696 88-89 21.31542153211095 28.00776486798523 28.617933011829287 22.05888058807453 90-91 21.306438660696834 27.84752920156755 28.507455475678256 22.338576662057356 92-93 21.15656432075887 27.97537672779413 28.66632428993859 22.201734661508407 94-95 21.251988536081846 27.84866348104015 28.5165256391128 22.382822343765206 96-97 21.37155625383347 27.979142726848487 28.403373135883204 22.24592788343483 98-99 21.230542071433923 27.98090921219008 28.560457439045067 22.228091277330925 100-101 21.524083227419595 27.807063129337283 28.284012447951508 22.384841195291617 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 146.0 1 131.0 2 142.5 3 145.0 4 128.0 5 148.5 6 224.0 7 344.5 8 455.5 9 577.0 10 712.0 11 839.5 12 1097.5 13 1496.5 14 2225.0 15 3055.0 16 4104.0 17 5698.0 18 7743.0 19 10331.0 20 13375.0 21 17133.5 22 21172.0 23 25860.0 24 32366.0 25 38810.0 26 45037.0 27 52487.5 28 61567.5 29 73075.5 30 87714.0 31 106943.5 32 135495.5 33 175842.5 34 226635.5 35 288462.0 36 364889.5 37 443350.0 38 507610.0 39 547609.5 40 559195.0 41 544651.0 42 499408.0 43 439035.5 44 382682.5 45 340024.5 46 309046.0 47 289040.0 48 277923.5 49 265509.0 50 253721.5 51 237829.0 52 215277.0 53 195034.0 54 179458.0 55 165594.0 56 152959.0 57 139689.0 58 124576.0 59 108310.0 60 92167.0 61 75838.5 62 60138.0 63 47021.5 64 36723.5 65 28552.0 66 22109.0 67 16751.5 68 12185.0 69 8716.5 70 6144.0 71 4494.5 72 3244.0 73 2291.5 74 1778.0 75 1448.0 76 1156.5 77 835.0 78 620.0 79 543.5 80 397.5 81 240.0 82 180.5 83 102.0 84 65.5 85 47.0 86 29.5 87 24.0 88 19.0 89 14.0 90 8.0 91 2.5 92 1.5 93 1.5 94 1.5 95 1.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09413301326408137 2 0.0022746685680344867 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 8.503433899194343E-5 10-11 6.377575424395757E-5 12-13 0.0 14-15 0.0014136958857410595 16-17 0.0 18-19 0.0 20-21 7.971969280494696E-5 22-23 1.48810093235901E-4 24-25 2.763616017238161E-4 26-27 0.0012648857925051584 28-29 0.0016528549641559003 30-31 0.003688364453775546 32-33 0.004262346241971164 34-35 0.005766391112891163 36-37 0.006893096104534414 38-39 0.011213903454562538 40-41 0.012085505429229959 42-43 0.012935848819149392 44-45 0.014987302247330029 46-47 0.014009407348922679 48-49 0.016746450135225858 50-51 0.015003246185891017 52-53 0.015906736037680416 54-55 0.016528549641559 56-57 0.014833177507907132 58-59 0.011750682719449181 60-61 0.006111843115045933 62-63 0.0032791366973768184 64-65 3.932838178377383E-4 66-67 0.0024447372460183734 68-69 0.003608644760970599 70-71 0.004315492703841128 72-73 0.017129104660689603 74-75 0.012096134721603952 76-77 0.03461960526209496 78-79 0.025531560282331014 80-81 0.019042377288008328 82-83 0.03177095490586486 84-85 0.011569984749091303 86-87 0.01566226231307858 88-89 0.007663719801648901 90-91 0.005840796159509114 92-93 0.012457530662319711 94-95 0.013100602850946283 96-97 0.007376728907551092 98-99 0.008599097530560278 100-101 0.0016050231484729322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9407964.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.250183896173773 #Duplication Level Percentage of deduplicated Percentage of total 1 34.921747863775124 4.27797833307059 2 1.9746855842037108 0.483805230872376 3 1.8911089802648324 0.6949929832784957 4 2.787204869954706 1.3657508885302503 5 3.913901484299072 2.3973006467085565 6 4.4633569684691174 3.2806166194809236 7 4.740727492202288 4.065234850678733 8 4.467188334986476 4.3779102881941325 9 4.243392473855889 4.6784104333536956 >10 36.028296114554664 68.96448711502751 >50 0.4729459958694432 3.7104681755433266 >100 0.09436117852025666 1.540496391060738 >500 1.620147671652877E-4 0.01776316069045143 >1k 9.206442772357813E-4 0.14478488351017457 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0629292373992927E-5 0.0 0.0 2 1.0629292373992927E-5 0.0 0.0 3 1.0629292373992927E-5 0.0 0.0 4 1.0629292373992927E-5 0.0 0.0 5 1.0629292373992927E-5 0.0 0.0 6 1.0629292373992927E-5 0.0 0.0 7 1.0629292373992927E-5 0.0 0.0 8 1.0629292373992927E-5 0.0 0.0 9 1.0629292373992927E-5 0.0 0.0 10-11 2.1258584747985854E-5 0.0 0.0 12-13 8.503433899194342E-5 0.0 0.0 14-15 1.7538332417088332E-4 0.0 0.0 16-17 2.9762018647180193E-4 0.0 0.0 18-19 3.7202523308975247E-4 0.0 0.0 20-21 4.623742182686924E-4 0.0 0.0 22-23 5.792964343826145E-4 0.0 0.0 24-25 7.918822818624731E-4 1.0629292373992927E-5 0.0 26-27 9.6195095984636E-4 1.0629292373992927E-5 0.0 28-29 0.0011692221611392221 1.0629292373992927E-5 0.0 30-31 0.0013818080086190807 1.0629292373992927E-5 0.0 32-33 0.0016475403179689037 1.0629292373992927E-5 0.0 34-35 0.001854811519261766 1.0629292373992927E-5 0.0 36-37 0.0023650175532134265 1.0629292373992927E-5 0.0 38-39 0.0026626377396852282 1.0629292373992927E-5 0.0 40-41 0.0030399776189619773 1.0629292373992927E-5 0.0 42-43 0.0035342397143526484 1.0629292373992927E-5 0.0 44-45 0.003885006362694415 1.0629292373992927E-5 1.169222161139222E-4 46-47 0.004299548765280139 1.0629292373992927E-5 1.169222161139222E-4 48-49 0.0048044401530448035 1.0629292373992927E-5 1.169222161139222E-4 50-51 0.0051870946785085486 1.0629292373992927E-5 1.169222161139222E-4 52-53 0.00551660274210233 1.0629292373992927E-5 1.169222161139222E-4 54-55 0.0058620547442571 1.0629292373992927E-5 1.169222161139222E-4 56-57 0.006250023915907841 3.188787712197878E-5 1.169222161139222E-4 58-59 0.006845264288851446 5.314646186996464E-5 1.169222161139222E-4 60-61 0.007711551617331869 5.314646186996464E-5 1.169222161139222E-4 62-63 0.008264274820779502 5.8461108056961106E-5 1.169222161139222E-4 64-65 0.00906147174882897 6.377575424395757E-5 1.169222161139222E-4 66-67 0.009683285352707557 6.377575424395757E-5 1.594393856098939E-4 68-69 0.010315728248960137 6.377575424395757E-5 2.0195655510586561E-4 70-71 0.010932227206651726 7.44050466179505E-5 2.0195655510586561E-4 72-73 0.011702850903766214 7.44050466179505E-5 2.0195655510586561E-4 74-75 0.012935848819149394 7.44050466179505E-5 2.0195655510586561E-4 76-77 0.01372241645482487 7.44050466179505E-5 2.0195655510586561E-4 78-79 0.014822548215533138 7.44050466179505E-5 2.0195655510586561E-4 80-81 0.015864218868184444 7.44050466179505E-5 2.0195655510586561E-4 82-83 0.01694840669033172 7.44050466179505E-5 2.0195655510586561E-4 84-85 0.018835106086715465 7.971969280494696E-5 2.0195655510586561E-4 86-87 0.0202913191419525 8.503433899194342E-5 2.0195655510586561E-4 88-89 0.022417177616751084 8.503433899194342E-5 2.0195655510586561E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 710 0.0 18.059834 3 ATGCCGT 1085 0.0 11.380254 4 TGCCGTC 1220 0.0 10.121017 5 GCCGTCT 1235 0.0 9.998091 6 CGTATGC 1295 0.0 9.544903 1 GTGCGGA 410 0.005545362 9.266917 8 GCGCGAG 210 0.0067468584 9.048535 84-85 GTATGCC 1655 2.3646862E-11 7.4609685 2 CGTCTTC 2050 7.6397555E-11 6.486842 8 ACGACAG 825 0.0052376795 6.332258 2 CCGTCTT 2220 5.4023985E-10 5.9898467 7 >>END_MODULE