FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n01 7-9252.34100000001b6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n01 7-9252.34100000001b6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9407964
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7100.018.0598343
ATGCCGT10850.011.3802544
TGCCGTC12200.010.1210175
GCCGTCT12350.09.9980916
CGTATGC12950.09.5449031
GTGCGGA4100.0055453629.2669178
GCGCGAG2100.00674685849.04853584-85
GTATGCC16552.3646862E-117.46096852
CGTCTTC20507.6397555E-116.4868428
ACGACAG8250.00523767956.3322582
CCGTCTT22205.4023985E-105.98984677
Produced by FastQC (version 0.11.2)