##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n01 6-9147.34100000001b46.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12714643 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.79655858210097 38.0 38.0 38.0 32.0 38.0 2 36.78514150967511 38.0 38.0 38.0 32.0 38.0 3 36.77185407407821 38.0 38.0 38.0 32.0 38.0 4 36.7710785902522 38.0 38.0 38.0 32.0 38.0 5 36.75929571911693 38.0 38.0 38.0 32.0 38.0 6 38.71080965466353 40.0 38.0 40.0 38.0 40.0 7 38.66076011729154 40.0 38.0 40.0 38.0 40.0 8 38.651237238827704 40.0 38.0 40.0 38.0 40.0 9 38.65097494282773 40.0 38.0 40.0 38.0 40.0 10-11 38.667099304321795 40.0 38.0 40.0 38.0 40.0 12-13 38.660193959043916 40.0 38.0 40.0 38.0 40.0 14-15 38.64611011886059 40.0 38.0 40.0 38.0 40.0 16-17 38.63093564640391 40.0 38.0 40.0 38.0 40.0 18-19 38.602374679336265 40.0 38.0 40.0 38.0 40.0 20-21 38.579880929413434 40.0 38.0 40.0 38.0 40.0 22-23 38.5612268075478 40.0 38.0 40.0 38.0 40.0 24-25 38.51882270701584 40.0 38.0 40.0 38.0 40.0 26-27 38.443367422899726 40.0 38.0 40.0 38.0 40.0 28-29 38.40575818762666 40.0 38.0 40.0 38.0 40.0 30-31 38.3562951000669 40.0 38.0 40.0 38.0 40.0 32-33 38.32266057332479 40.0 38.0 40.0 38.0 40.0 34-35 38.29256491904649 40.0 38.0 40.0 38.0 40.0 36-37 38.25235848147683 40.0 38.0 40.0 38.0 40.0 38-39 38.20168116399336 40.0 38.0 40.0 38.0 40.0 40-41 38.15655087602538 40.0 38.0 40.0 38.0 40.0 42-43 38.11878272948756 40.0 38.0 40.0 38.0 40.0 44-45 38.095115372094995 40.0 38.0 40.0 38.0 40.0 46-47 38.05876094987488 40.0 38.0 40.0 38.0 40.0 48-49 38.014355062898744 40.0 38.0 40.0 38.0 40.0 50-51 37.96085568426892 40.0 38.0 40.0 38.0 40.0 52-53 37.91583845492162 40.0 38.0 40.0 38.0 40.0 54-55 37.86586984785967 40.0 38.0 40.0 32.0 40.0 56-57 37.812439444819645 40.0 38.0 40.0 32.0 40.0 58-59 37.78593303799407 40.0 38.0 40.0 32.0 40.0 60-61 37.72410884835697 40.0 38.0 40.0 32.0 40.0 62-63 37.686571931276404 40.0 38.0 40.0 32.0 40.0 64-65 37.643607335259034 40.0 38.0 40.0 32.0 40.0 66-67 37.553394381580354 40.0 38.0 40.0 32.0 40.0 68-69 37.49558422521183 40.0 38.0 40.0 32.0 40.0 70-71 37.471871683695724 40.0 38.0 40.0 32.0 40.0 72-73 37.420862426101934 40.0 38.0 40.0 32.0 40.0 74-75 37.3491929344772 40.0 38.0 40.0 32.0 40.0 76-77 37.27766225917629 38.0 38.0 40.0 32.0 40.0 78-79 37.191010750360824 38.0 38.0 40.0 32.0 40.0 80-81 37.148529180095736 38.0 38.0 40.0 32.0 40.0 82-83 37.06792412496363 38.0 38.0 40.0 32.0 40.0 84-85 36.99604043149304 38.0 38.0 40.0 32.0 40.0 86-87 36.93371532334805 38.0 38.0 40.0 32.0 40.0 88-89 36.88614045239021 38.0 38.0 40.0 32.0 40.0 90-91 36.81790566199932 38.0 38.0 40.0 32.0 40.0 92-93 36.74678750319612 38.0 38.0 40.0 32.0 40.0 94-95 36.660248541779744 38.0 38.0 40.0 32.0 40.0 96-97 36.573211611210795 38.0 38.0 40.0 32.0 40.0 98-99 36.46639252867737 38.0 38.0 40.0 32.0 40.0 100-101 35.587742259063035 38.0 35.0 39.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.1560949900766886 2201 2 0.1574484355120802 2201 3 0.11033583747978781 2201 4 0.04181089117800241 2201 5 0.11862725078200498 2201 6 0.11990341738720645 2201 7 0.20152900907520177 2201 8 0.16311175281001766 2201 9 0.126083425002534 2201 10-11 0.09194631342766968 2201 12-13 0.05564481133740884 2201 14-15 0.06179656611982409 2201 16-17 0.06511747061782103 2201 18-19 0.04179041220121604 2201 20-21 0.054262129544291327 2201 22-23 0.11237846236240046 2201 24-25 0.046203340159635786 2201 26-27 0.03579684115838688 2201 28-29 0.030057768579844435 2201 30-31 0.054445646056045405 2201 32-33 0.0523638345036872 2201 34-35 0.005229973988441827 2201 36-37 0.03395585120300382 2201 38-39 0.05830087019935348 2201 40-41 0.03533386866376276 2201 42-43 -0.043617832467774065 2201 44-45 -0.025747204378433253 2201 46-47 -0.06537960108013863 2201 48-49 -0.038508625939556396 2201 50-51 -0.056082531968861815 2201 52-53 -0.04336922403163612 2201 54-55 -0.18180268401928146 2201 56-57 -0.14389077305806097 2201 58-59 -0.12293030266602045 2201 60-61 -0.10388761992711437 2201 62-63 -0.09832418902986007 2201 64-65 -0.15536773900596756 2201 66-67 -0.07836809754925866 2201 68-69 -0.08671625533781935 2201 70-71 -0.10045525114093579 2201 72-73 -0.1178386071851989 2201 74-75 -0.08233955406215188 2201 76-77 -0.1199043330857208 2201 78-79 -0.10814277734237265 2201 80-81 -0.13950908880988777 2201 82-83 -0.19936597850420412 2201 84-85 -0.2567515940394358 2201 86-87 -0.2992634095656683 2201 88-89 -0.30201285489854257 2201 90-91 -0.2661056214436144 2201 92-93 -0.2940177330337903 2201 94-95 -0.2689049114965343 2201 96-97 -0.2576480634770064 2201 98-99 -0.34071762405032047 2201 100-101 -0.4464763742631561 1101 1 0.14333004576630515 1101 2 0.12776813197433512 1101 3 0.10061945233837122 1101 4 0.06503275940644215 1101 5 0.05167653988424803 1101 6 0.09992966636029621 1101 7 0.10930236908688329 1101 8 0.04440744162371857 1101 9 0.06549816163974498 1101 10-11 0.07976441405541124 1101 12-13 0.05746074220271424 1101 14-15 0.08520649950983739 1101 16-17 0.024920603319692702 1101 18-19 0.05742453815621218 1101 20-21 0.02737043589871746 1101 22-23 0.03468518748097438 1101 24-25 0.046462402066559605 1101 26-27 -0.03565875313611144 1101 28-29 0.02313688475945952 1101 30-31 0.02426043622169516 1101 32-33 -0.006120766766677832 1101 34-35 -0.011637875170130485 1101 36-37 -9.592690069837317E-4 1101 38-39 6.10107147146266E-4 1101 40-41 0.026709914543644686 1101 42-43 0.014065581158220652 1101 44-45 -0.05497936466605324 1101 46-47 -0.03518083023051588 1101 48-49 -0.04725922348297473 1101 50-51 -0.06201778402483882 1101 52-53 -0.11990896818286956 1101 54-55 -0.05261262370675723 1101 56-57 -0.07373117529423467 1101 58-59 -0.06701391437587034 1101 60-61 -0.11451818924644641 1101 62-63 -0.10538931339045376 1101 64-65 -0.14357796408304324 1101 66-67 -0.1123001550737257 1101 68-69 -0.12749616041786282 1101 70-71 -0.10796638620149679 1101 72-73 -0.15751515371343316 1101 74-75 -0.19144744678771985 1101 76-77 -0.17645458255115187 1101 78-79 -0.20902488023720167 1101 80-81 -0.20179239165777574 1101 82-83 -0.32064877898623934 1101 84-85 -0.2710741944911348 1101 86-87 -0.21991442417292006 1101 88-89 -0.2967993851965005 1101 90-91 -0.3083807018078346 1101 92-93 -0.36416819947546486 1101 94-95 -0.3468676390997487 1101 96-97 -0.32763541130022134 1101 98-99 -0.3832067036760236 1101 100-101 -0.487463222583024 2202 1 0.0019985094116421465 2202 2 0.09895951168999773 2202 3 0.03774983407086552 2202 4 0.025894290686323984 2202 5 0.054973785526165386 2202 6 0.07715169583934056 2202 7 0.07907955279050327 2202 8 0.03414807424641708 2202 9 0.010444604441616434 2202 10-11 0.0705762128722256 2202 12-13 0.047956268993289086 2202 14-15 -0.008646350643431333 2202 16-17 -0.006551770238225174 2202 18-19 -0.00909920862491731 2202 20-21 -0.05479359096920433 2202 22-23 -0.025931103757514506 2202 24-25 -0.02685576390972244 2202 26-27 -0.01780852539232569 2202 28-29 -0.004128733554786379 2202 30-31 0.012382055710098427 2202 32-33 -0.04899671853365106 2202 34-35 -0.13104758786433024 2202 36-37 -0.08210571126419097 2202 38-39 -0.04516986049229388 2202 40-41 -0.051208325024731494 2202 42-43 -0.10235793176195784 2202 44-45 -0.10471484463357683 2202 46-47 -0.17132721162876408 2202 48-49 -0.17346143442480155 2202 50-51 -0.21099706406648977 2202 52-53 -0.16124160013761468 2202 54-55 -0.16308804418618905 2202 56-57 -0.23112620287605523 2202 58-59 -0.19644913558586552 2202 60-61 -0.1576850360375559 2202 62-63 -0.2540622509330106 2202 64-65 -0.2850052803403784 2202 66-67 -0.15023479411504326 2202 68-69 -0.1771678652748463 2202 70-71 -0.18200174297410854 2202 72-73 -0.18319644710678773 2202 74-75 -0.17427619236341485 2202 76-77 -0.2624182227868701 2202 78-79 -0.2379790426915065 2202 80-81 -0.30767687780902975 2202 82-83 -0.2784771063303211 2202 84-85 -0.3090173909542102 2202 86-87 -0.30649538677564436 2202 88-89 -0.3114600289388747 2202 90-91 -0.37547800600495407 2202 92-93 -0.36431487927710293 2202 94-95 -0.42399641265527777 2202 96-97 -0.45981698479065614 2202 98-99 -0.4720013759360455 2202 100-101 -0.4876996033394718 1102 1 0.06691387857875242 1102 2 0.018531269366462766 1102 3 -0.02500033493585363 1102 4 -0.08565507436928499 1102 5 -0.07297695398075632 1102 6 -0.03177869702213343 1102 7 -0.020343494547489627 1102 8 0.020802429815333312 1102 9 -0.034916109646573545 1102 10-11 -0.06202072270574632 1102 12-13 -0.06830283633824052 1102 14-15 -0.06356026801502423 1102 16-17 -0.049299794249158424 1102 18-19 -0.06304704987128673 1102 20-21 -0.05809723262498778 1102 22-23 -0.0865387508764428 1102 24-25 -0.048277122079618096 1102 26-27 -0.08848573532276305 1102 28-29 -0.11590928220479668 1102 30-31 -0.12229890525708953 1102 32-33 -0.06831254988033209 1102 34-35 -0.11400479065354574 1102 36-37 -0.13056544610040532 1102 38-39 -0.1068777805900325 1102 40-41 -0.15157792990000019 1102 42-43 -0.19048967155264052 1102 44-45 -0.21945404343190233 1102 46-47 -0.25090226966867135 1102 48-49 -0.25808302871017474 1102 50-51 -0.2712856660059657 1102 52-53 -0.18069963084040808 1102 54-55 -0.2590232449938483 1102 56-57 -0.2896608276234218 1102 58-59 -0.30510052795305853 1102 60-61 -0.2653315420049154 1102 62-63 -0.27877097821334473 1102 64-65 -0.3680330118292616 1102 66-67 -0.27950123918407144 1102 68-69 -0.3288458164916932 1102 70-71 -0.3736617089136587 1102 72-73 -0.4129242244239677 1102 74-75 -0.43476313918962006 1102 76-77 -0.4323830521777339 1102 78-79 -0.33958185928250373 1102 80-81 -0.4227550857108042 1102 82-83 -0.4650137214825989 1102 84-85 -0.43418684259053464 1102 86-87 -0.4582006897536459 1102 88-89 -0.4516837185605027 1102 90-91 -0.5319888236019494 1102 92-93 -0.5492997133495123 1102 94-95 -0.6271444095315317 1102 96-97 -0.7099308782150189 1102 98-99 -0.8577724255715395 1102 100-101 -0.7967829913156521 2203 1 0.07331792373090451 2203 2 -0.07011527253278871 2203 3 -0.014135489587729921 2203 4 -0.1068590468492161 2203 5 -0.02996393448362511 2203 6 -0.1359042779457198 2203 7 -0.06803484938233595 2203 8 -0.09520848956137229 2203 9 -0.0019902302875109967 2203 10-11 -0.056230022657707934 2203 12-13 -0.06943503847070787 2203 14-15 -0.08434819848434927 2203 16-17 -0.14000938659915363 2203 18-19 -0.14602896978210822 2203 20-21 -0.06806724177215528 2203 22-23 -0.0685225612717062 2203 24-25 -0.1189323175784125 2203 26-27 -0.1789257682116272 2203 28-29 -0.10876309959104447 2203 30-31 -0.10019446403499188 2203 32-33 -0.0519822360688309 2203 34-35 -0.13610183014942834 2203 36-37 -0.18915192637391698 2203 38-39 -0.18306969279940688 2203 40-41 -0.18287561685590958 2203 42-43 -0.14298489526713354 2203 44-45 -0.20697792154327033 2203 46-47 -0.21856607277150886 2203 48-49 -0.26254357687604823 2203 50-51 -0.22139576635181868 2203 52-53 -0.23062453780764258 2203 54-55 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0.05644852196325445 1103 12-13 0.03612055075219445 1103 14-15 0.02511961782241201 1103 16-17 0.0536510276357447 1103 18-19 0.04256618405626256 1103 20-21 0.06308926259132619 1103 22-23 0.037182846593637464 1103 24-25 0.04836032270917201 1103 26-27 0.005639111217227821 1103 28-29 -0.028089795969279407 1103 30-31 0.04319266431651414 1103 32-33 -0.04453732795007426 1103 34-35 -0.031292378483314565 1103 36-37 -0.03267727776335505 1103 38-39 0.010057500302451672 1103 40-41 0.00648136626737994 1103 42-43 0.014082654335972222 1103 44-45 -0.04468767183792011 1103 46-47 -0.08983432826553894 1103 48-49 -0.06714209163182261 1103 50-51 -0.12516888734225518 1103 52-53 -0.062233695018917956 1103 54-55 -0.06589686135892947 1103 56-57 -0.037418151771788644 1103 58-59 -0.006584879507144592 1103 60-61 -0.06119816747474971 1103 62-63 -0.1014448921108837 1103 64-65 -0.1474141682125918 1103 66-67 -0.0916544074929675 1103 68-69 -0.12272695096491049 1103 70-71 -0.11863990050302675 1103 72-73 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0.10473407040944238 2204 30-31 0.07863093870439286 2204 32-33 0.06755834427385565 2204 34-35 0.07962499153833846 2204 36-37 0.02355937409613773 2204 38-39 0.049151300175182655 2204 40-41 0.05050418617935293 2204 42-43 0.025041419675588372 2204 44-45 0.05329882383966833 2204 46-47 0.06398527642272711 2204 48-49 0.021012459376045456 2204 50-51 0.06805479355930544 2204 52-53 0.09235295666908172 2204 54-55 0.050117207303067346 2204 56-57 0.07233292002541702 2204 58-59 0.06715066752849452 2204 60-61 -9.067748188513747E-4 2204 62-63 0.0841887598099973 2204 64-65 0.0384053298853857 2204 66-67 0.10009298614896522 2204 68-69 0.07102626068274276 2204 70-71 0.09343880184920295 2204 72-73 0.12689600192353367 2204 74-75 0.043468621804684915 2204 76-77 0.027872542523248 2204 78-79 0.07178661218716087 2204 80-81 0.07115675742663541 2204 82-83 -0.021505817496418445 2204 84-85 0.03981372211312362 2204 86-87 0.013022518818210926 2204 88-89 0.009953855724397442 2204 90-91 -0.004837805783438398 2204 92-93 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0.41419997426913824 1214 98-99 0.38454315680004925 1214 100-101 0.565504319189877 1215 1 -0.1780767696301595 1215 2 -0.1851294776556145 1215 3 -0.0821934234023729 1215 4 -0.07901240376341434 1215 5 -0.07053060675578138 1215 6 -0.06217529648174036 1215 7 -0.10521911439727916 1215 8 -0.06235958378272244 1215 9 -0.09080660671776286 1215 10-11 -0.09628302778993003 1215 12-13 -0.12850519837288488 1215 14-15 -0.08117121268499261 1215 16-17 -0.025102974272812162 1215 18-19 -0.04718745469665464 1215 20-21 -0.02705115583540163 1215 22-23 -0.0493512672669425 1215 24-25 -0.03300130454444172 1215 26-27 -0.017303270907959245 1215 28-29 -0.013968920401040918 1215 30-31 -0.05088083471067506 1215 32-33 -0.041236646872171434 1215 34-35 -0.03515643998419904 1215 36-37 -0.00917615625959911 1215 38-39 -0.003085685448056097 1215 40-41 -0.025113417707125052 1215 42-43 0.010159384211860356 1215 44-45 -3.983601658319458E-5 1215 46-47 -0.013057089860240012 1215 48-49 0.059566358752135784 1215 50-51 0.09168387497840769 1215 52-53 0.07704790471412082 1215 54-55 0.10615166486573457 1215 56-57 0.0267222860664873 1215 58-59 0.06497253656016966 1215 60-61 0.15401459317084942 1215 62-63 0.16441228315147782 1215 64-65 0.22115954426611495 1215 66-67 0.2557473875568519 1215 68-69 0.23187265593132622 1215 70-71 0.18273684340654484 1215 72-73 0.16816986517867605 1215 74-75 0.2093888357589151 1215 76-77 0.22406069818354268 1215 78-79 0.2847569338957783 1215 80-81 0.2762262616468121 1215 82-83 0.28714143315600893 1215 84-85 0.2777378806389805 1215 86-87 0.3636360363639142 1215 88-89 0.3386081369522387 1215 90-91 0.38777342066509846 1215 92-93 0.4227462293603139 1215 94-95 0.3638042763514804 1215 96-97 0.39657152075263014 1215 98-99 0.4544456123534104 1215 100-101 0.541223387171371 1212 1 -0.1817819793892994 1212 2 -0.11176780971414502 1212 3 -0.12398134085265156 1212 4 -0.09540065351594507 1212 5 -0.09437670824325295 1212 6 -0.1437609495706127 1212 7 -0.20805496918774935 1212 8 -0.262336936718917 1212 9 -0.3236974674504296 1212 10-11 -0.3312886017008836 1212 12-13 -0.2505771844572635 1212 14-15 -0.21690508360366323 1212 16-17 -0.13841548625809708 1212 18-19 -0.11149307682266141 1212 20-21 -0.09734992875993953 1212 22-23 -0.07015214424738048 1212 24-25 -0.06467838558128847 1212 26-27 -0.08498147546511348 1212 28-29 -0.05638832567451857 1212 30-31 -0.024999085743978355 1212 32-33 -0.12374890567587471 1212 34-35 -0.13696390806008907 1212 36-37 -0.194369593895658 1212 38-39 -0.2186980486522998 1212 40-41 -0.3263962863008345 1212 42-43 -0.36420273502182 1212 44-45 -0.1491488224712043 1212 46-47 -0.1352530263499716 1212 48-49 -0.315806221745639 1212 50-51 -0.19195329066536004 1212 52-53 -0.2843720733890365 1212 54-55 -0.380526941928899 1212 56-57 -0.3593526593378442 1212 58-59 -0.2779327721955269 1212 60-61 -0.34999327560340276 1212 62-63 -0.34205667870450185 1212 64-65 -0.07526717535692029 1212 66-67 -0.10083987620681967 1212 68-69 -0.2450749645452106 1212 70-71 -0.1607417878623849 1212 72-73 -0.1552996132494755 1212 74-75 -0.06693077887100429 1212 76-77 -0.19450837870655135 1212 78-79 0.004131873228921279 1212 80-81 0.05338332007679014 1212 82-83 0.07302183615015423 1212 84-85 0.09596152441242367 1212 86-87 0.09192436282797445 1212 88-89 0.04612943666927549 1212 90-91 0.1935249797215377 1212 92-93 0.20764374826908494 1212 94-95 0.08803495902493097 1212 96-97 0.184384728763618 1212 98-99 0.15724576598496753 1212 100-101 0.19308484673445037 1213 1 -0.2786405930999294 1213 2 -0.16502426392254677 1213 3 -0.13421411773914116 1213 4 -0.14456847497624636 1213 5 -0.11773912786924257 1213 6 -0.04304260106934521 1213 7 -0.054216173732839934 1213 8 -0.12060746889301299 1213 9 -0.10083524026344293 1213 10-11 -0.15650989148097239 1213 12-13 -0.15654508224052677 1213 14-15 -0.2573731173941809 1213 16-17 -0.3826786057264542 1213 18-19 -0.34079867829625954 1213 20-21 -0.1966464942371431 1213 22-23 -0.30814727831251076 1213 24-25 -0.36616495782213576 1213 26-27 -0.1994261357997189 1213 28-29 -0.20548945410023123 1213 30-31 -0.21781002406138583 1213 32-33 -0.2425204198369002 1213 34-35 -0.15087975750387272 1213 36-37 -0.06695576129280312 1213 38-39 -0.03984783472418485 1213 40-41 -0.04056315771437369 1213 42-43 -0.014791915647364817 1213 44-45 -0.04895949146975909 1213 46-47 -0.024384964879160975 1213 48-49 0.015045094463729924 1213 50-51 0.010506198095619368 1213 52-53 0.02563142335765889 1213 54-55 0.0612973674144115 1213 56-57 0.03871860296590057 1213 58-59 0.07210419662999357 1213 60-61 0.057601807371661096 1213 62-63 0.11148362968525305 1213 64-65 0.08668911706509874 1213 66-67 0.11646021024264996 1213 68-69 0.1190907124376821 1213 70-71 0.08504084069302564 1213 72-73 0.03203082769955756 1213 74-75 0.0762731661445386 1213 76-77 0.130320654552321 1213 78-79 0.1487078226842371 1213 80-81 0.13000085139596962 1213 82-83 0.23982029187813225 1213 84-85 0.14299615747428618 1213 86-87 0.1397053350352806 1213 88-89 0.1985973591569561 1213 90-91 0.2447597603075522 1213 92-93 0.2654083047252911 1213 94-95 0.32605114149406944 1213 96-97 0.2750136994474559 1213 98-99 0.2517902895887403 1213 100-101 0.17334059428682735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 22.0 15 55.0 16 136.0 17 270.0 18 762.0 19 1759.0 20 4006.0 21 8667.0 22 15278.0 23 24606.0 24 36077.0 25 49800.0 26 66525.0 27 87092.0 28 109375.0 29 134658.0 30 164381.0 31 196819.0 32 237513.0 33 288294.0 34 356925.0 35 460677.0 36 640592.0 37 1018214.0 38 2228282.0 39 6583853.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.601066660452826 29.560678790794864 29.582374753834266 21.25587979491804 2 20.37848833697436 30.27835123224371 29.280786901873533 20.0623735289084 3 20.328639978330497 28.78523604634436 29.872769530375333 21.01335444494981 4 20.603417650027612 28.41566216212284 29.744248422861734 21.23667176498782 5 21.15306737279214 28.723716426800188 29.3876438371097 20.735572363297972 6 20.997191977784986 28.93592844093224 28.66508324299786 21.40179633828492 7 21.058939680807395 29.385292217799584 29.057190201879834 20.498577899513183 8 20.37384769670686 29.11690874844068 29.660046294654123 20.84919726019834 9 20.42792259251464 28.542279936287596 29.94953217693373 21.080265294264027 10-11 20.605625745671258 28.96465486286688 29.26215456684972 21.167564824612143 12-13 20.31929248819648 28.9575216543634 29.72398831803614 20.999197539403976 14-15 20.443141571891772 28.58634015363362 29.755700778549983 21.214817495924624 16-17 20.501118277563908 28.785255708713176 29.629829166261295 21.08379684746162 18-19 20.400230663181027 28.558957573563013 29.740795710898055 21.3000160523579 20-21 20.552124621484253 28.5183142772662 29.475522664659636 21.454038436589915 22-23 20.536289762202074 28.46331061905984 29.61312486044607 21.387274758292012 24-25 20.64333821512162 28.37462954997494 29.493964538255735 21.488067696647704 26-27 20.813150302065143 28.412629824461504 29.276845542042 21.497374331431356 28-29 20.757914950916614 28.53841435102757 29.333097748075225 21.37057294998059 30-31 21.000559297708957 28.28144983871918 29.16678562852643 21.55120523504543 32-33 20.981777542588176 28.493245919567045 29.237357256813933 21.287619281030842 34-35 21.066124488015685 28.261573434189952 29.18483921188404 21.48746286591032 36-37 20.973166846917707 28.42054075312162 29.078045423262218 21.528246976698455 38-39 20.987346970575977 28.306479552493492 29.210119041228054 21.49605443570248 40-41 21.157018611173832 28.31986106287233 28.963532346998132 21.559587978955705 42-43 21.024338543431593 28.361947341478068 29.11719763479977 21.496516480290577 44-45 20.97509080265504 28.175418442641842 29.058049196970533 21.791441557732586 46-47 21.088684412466645 28.402580271447427 28.928807733373052 21.579927582712873 48-49 20.90986537702143 28.1530402264221 29.159007562611407 21.778086833945064 50-51 21.055786520612443 28.24267408044768 28.877687598206254 21.82385180073362 52-53 21.026302996549884 28.28803189271763 29.093404821350756 21.59226028938173 54-55 21.130442409446733 28.04901772225782 28.98256014039675 21.837979727898702 56-57 21.198240526669952 28.264860841036242 28.887277736976756 21.64962089531705 58-59 21.002760094753853 28.1605295241333 29.10801299803695 21.728697383075897 60-61 21.09389759986157 28.146855594489473 28.782815590871376 21.976431214777588 62-63 21.09876153007388 28.167969560640344 29.017335071075863 21.71593383820991 64-65 21.17383901177769 28.0708927386836 28.914820955471743 21.84044729406697 66-67 21.201139772604694 28.182233212305473 28.757102097045838 21.85952491804399 68-69 20.959083049554888 28.11085826934161 29.013392486352206 21.916666194751294 70-71 21.280579840571686 28.099713267509642 28.693958301700185 21.925748590218486 72-73 21.19940021021905 28.159468659630942 28.85406311008087 21.78706802006914 74-75 21.18552633260803 28.049279925116537 28.817916969422257 21.947276772853172 76-77 21.15473133852836 28.27312434873186 28.683323212275464 21.88882110046432 78-79 21.15256988340153 28.111644773837735 28.90256602856856 21.833219314192174 80-81 21.32193118608338 28.063885567267953 28.682780835085005 21.93140241156366 82-83 21.137780685598102 28.343711128506104 28.877531395864292 21.640976790031495 84-85 21.187039849736934 28.080100082036967 28.70799223529646 22.024867832929637 86-87 21.039975278108496 28.342321580536566 28.716670077319627 21.901033064035307 88-89 21.107266109387655 28.114295067584806 28.780592340363814 21.99784648266373 90-91 21.15309447714426 28.05704267645345 28.695789237349135 22.094073609053158 92-93 21.047142793597125 28.143876464755163 28.82389279606507 21.985087945582645 94-95 21.083091136539288 28.048219557114663 28.68635896343535 22.1823303429107 96-97 21.193371996985764 28.201591932962515 28.551097801196327 22.053938268855394 98-99 21.026222133725238 28.108832829468174 28.773945852353556 22.090999184453032 100-101 21.365914607095764 28.02997111827296 28.420652878905685 22.183461395725594 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 89.0 1 84.5 2 96.0 3 112.5 4 99.5 5 110.0 6 148.5 7 224.0 8 291.5 9 323.5 10 406.5 11 556.5 12 786.5 13 1074.5 14 1539.5 15 2422.0 16 3384.0 17 4900.0 18 7132.0 19 9961.0 20 14760.0 21 20474.0 22 26378.0 23 34319.0 24 43365.5 25 53836.0 26 65175.5 27 78695.5 28 94584.5 29 112892.5 30 136339.5 31 164425.5 32 205528.0 33 262350.5 34 328313.0 35 412703.0 36 515390.5 37 614349.5 38 696008.5 39 748181.5 40 762072.5 41 734102.0 42 669947.0 43 589238.0 44 512217.0 45 451808.5 46 405487.5 47 374222.0 48 357004.0 49 344215.5 50 328344.5 51 306227.5 52 279558.5 53 253533.0 54 232696.5 55 216506.0 56 200022.0 57 181867.0 58 163145.5 59 142132.0 60 120090.5 61 97178.5 62 77686.0 63 62447.5 64 48632.5 65 37646.5 66 28756.5 67 21135.5 68 15370.5 69 11046.5 70 7629.0 71 5369.5 72 4077.0 73 3077.5 74 2170.0 75 1649.5 76 1216.5 77 904.0 78 720.0 79 565.5 80 389.0 81 247.0 82 173.5 83 106.0 84 73.0 85 49.0 86 36.0 87 31.0 88 21.5 89 10.5 90 7.0 91 6.0 92 3.0 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09295581480345143 2 0.0021628605695024235 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 5.50546326782435E-5 10-11 6.685205396643854E-5 12-13 0.0 14-15 0.0013645683956678926 16-17 0.0 18-19 0.0 20-21 1.0617679159375534E-4 22-23 1.8875874061112058E-4 24-25 3.145979010185343E-4 26-27 0.0013134462367523807 28-29 0.00163984155905911 30-31 0.003806634602324265 32-33 0.00433358608653031 34-35 0.005918373012911177 36-37 0.007023398140238778 38-39 0.011345186805480894 40-41 0.01222606092833279 42-43 0.012890648994234442 44-45 0.015014184826109549 46-47 0.014113648334443995 48-49 0.01653998464604944 50-51 0.015112496670177843 52-53 0.015910788844012373 54-55 0.016500659908422123 56-57 0.014640599818650039 58-59 0.01173843418175406 60-61 0.006236903387692443 62-63 0.003511699070119389 64-65 4.2863964013775295E-4 66-67 0.0027723940027258335 68-69 0.0039246088152062155 70-71 0.004148759819681921 72-73 0.01721637013323929 74-75 0.012233925875858252 76-77 0.034169264524375555 78-79 0.02616668041721659 80-81 0.019473610073047272 82-83 0.032179432800433325 84-85 0.011581135231244793 86-87 0.015914721317775105 88-89 0.008049773792311747 90-91 0.005965562698063956 92-93 0.012894581467997174 94-95 0.013122664946235612 96-97 0.0077037161011913585 98-99 0.008568860328992327 100-101 0.0015926518739063297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2714643E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.079314291081001 #Duplication Level Percentage of deduplicated Percentage of total 1 37.04418340979893 4.84512517473209 2 2.37537910155615 0.6213665965943701 3 2.312767720971565 0.9074824771456269 4 2.9711043719237615 1.554400314879828 5 4.127575980809343 2.6992931756661163 6 4.677497439342073 3.6707075462928938 7 5.3972519369997665 4.941464807451595 8 4.700291033859888 4.918126695312282 9 4.285165077719455 5.044231875659157 >10 31.49445069108534 64.13307212573622 >50 0.49853094888398275 4.431465174443151 >100 0.11513850578656966 2.123317372975591 >500 1.312170364195768E-4 0.016613095361662392 >1k 5.325642267369072E-4 0.09333356774938072 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.5729895050926714E-5 0.0 0.0 2 1.5729895050926714E-5 0.0 0.0 3 1.5729895050926714E-5 0.0 0.0 4 1.5729895050926714E-5 0.0 0.0 5 1.5729895050926714E-5 0.0 0.0 6 1.5729895050926714E-5 0.0 0.0 7 1.5729895050926714E-5 0.0 0.0 8 2.3594842576390072E-5 0.0 0.0 9 2.3594842576390072E-5 0.0 0.0 10-11 6.685205396643854E-5 3.9324737627316784E-6 0.0 12-13 9.04468965428286E-5 7.864947525463357E-6 0.0 14-15 1.2190668664468204E-4 7.864947525463357E-6 0.0 16-17 1.6516389803473053E-4 7.864947525463357E-6 0.0 18-19 3.4212521735765605E-4 7.864947525463357E-6 0.0 20-21 5.308839579687766E-4 7.864947525463357E-6 0.0 22-23 7.078452772917022E-4 1.5729895050926714E-5 0.0 24-25 9.555911243437978E-4 1.5729895050926714E-5 0.0 26-27 0.0012151343926840887 2.3594842576390072E-5 0.0 28-29 0.0014982725036007697 2.3594842576390072E-5 0.0 30-31 0.001836465247195694 2.3594842576390072E-5 0.0 32-33 0.0021274683056378384 2.3594842576390072E-5 0.0 34-35 0.0025757703145892494 2.3594842576390072E-5 0.0 36-37 0.0028353135829295405 2.3594842576390072E-5 7.864947525463358E-5 38-39 0.0032639532230672934 2.3594842576390072E-5 2.988680059676076E-4 40-41 0.003492036701305731 2.3594842576390072E-5 2.988680059676076E-4 42-43 0.003696525336967778 2.3594842576390072E-5 2.988680059676076E-4 44-45 0.0040543804493763606 2.3594842576390072E-5 2.988680059676076E-4 46-47 0.004380775771683091 2.3594842576390072E-5 2.988680059676076E-4 48-49 0.004797617990532648 2.3594842576390072E-5 2.988680059676076E-4 50-51 0.0055251256366380085 2.3594842576390072E-5 2.988680059676076E-4 52-53 0.0058751158015211274 2.3594842576390072E-5 2.988680059676076E-4 54-55 0.006402067285727174 2.3594842576390072E-5 2.988680059676076E-4 56-57 0.006795314662000341 2.3594842576390072E-5 3.2246284854399763E-4 58-59 0.007361590883833702 2.752731633912175E-5 3.2246284854399763E-4 60-61 0.007841352682886967 3.145979010185343E-5 3.2246284854399763E-4 62-63 0.008576725276517792 3.145979010185343E-5 3.2246284854399763E-4 64-65 0.009390747345403249 3.145979010185343E-5 3.2246284854399763E-4 66-67 0.00992949625089749 3.145979010185343E-5 3.2246284854399763E-4 68-69 0.010865425006427627 3.145979010185343E-5 3.2246284854399763E-4 70-71 0.01185247592087328 3.145979010185343E-5 4.797617990532648E-4 72-73 0.012658633042233275 3.145979010185343E-5 4.797617990532648E-4 74-75 0.013264234001693952 3.145979010185343E-5 5.662762218333618E-4 76-77 0.014042863806714824 3.5392263864585105E-5 5.662762218333618E-4 78-79 0.014790033821633844 3.932473762731679E-5 6.37060749562532E-4 80-81 0.015619785785570228 4.325721139004847E-5 6.37060749562532E-4 82-83 0.016811325335677925 6.685205396643854E-5 6.37060749562532E-4 84-85 0.018116906624904845 8.651442278009693E-5 6.37060749562532E-4 86-87 0.019473610073047272 8.651442278009693E-5 6.37060749562532E-4 88-89 0.02096795010288531 8.651442278009693E-5 6.37060749562532E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAACG 120 0.004770224 19.7887 9 TATGCCG 1125 0.0 10.131415 3 GCCGTCT 1345 1.2369128E-10 8.12114 6 CGTATGC 1630 1.6370905E-11 7.5818725 1 TGCCGTC 1425 3.4033292E-9 7.3319454 5 CGCCGGC 370 0.0016709407 7.0605574 46-47 ATGCCGT 1665 1.0944859E-8 6.56032 4 GTATGCC 2270 9.313226E-10 5.857945 2 >>END_MODULE