FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n01 6-9147.34100000001b46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n01 6-9147.34100000001b46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12714643
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG1200.00477022419.78879
TATGCCG11250.010.1314153
GCCGTCT13451.2369128E-108.121146
CGTATGC16301.6370905E-117.58187251
TGCCGTC14253.4033292E-97.33194545
CGCCGGC3700.00167094077.060557446-47
ATGCCGT16651.0944859E-86.560324
GTATGCC22709.313226E-105.8579452