##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n01 5-59290.34100000001b2c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12122790 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.914666178330236 38.0 38.0 38.0 32.0 38.0 2 36.91776967183297 38.0 38.0 38.0 32.0 38.0 3 36.89796911437054 38.0 38.0 38.0 32.0 38.0 4 36.886822092934054 38.0 38.0 38.0 32.0 38.0 5 36.864149341859424 38.0 38.0 38.0 32.0 38.0 6 38.78257901027734 40.0 38.0 40.0 38.0 40.0 7 38.72722954039458 40.0 38.0 40.0 38.0 40.0 8 38.71328291589642 40.0 38.0 40.0 38.0 40.0 9 38.70979518741148 40.0 38.0 40.0 38.0 40.0 10-11 38.724453199304776 40.0 38.0 40.0 38.0 40.0 12-13 38.71236897611853 40.0 38.0 40.0 38.0 40.0 14-15 38.693336434929584 40.0 38.0 40.0 38.0 40.0 16-17 38.67553240631901 40.0 38.0 40.0 38.0 40.0 18-19 38.64131511805451 40.0 38.0 40.0 38.0 40.0 20-21 38.61758357605798 40.0 38.0 40.0 38.0 40.0 22-23 38.60018135264242 40.0 38.0 40.0 38.0 40.0 24-25 38.554676563728314 40.0 38.0 40.0 38.0 40.0 26-27 38.47940997905597 40.0 38.0 40.0 38.0 40.0 28-29 38.44084884750127 40.0 38.0 40.0 38.0 40.0 30-31 38.39388020414442 40.0 38.0 40.0 38.0 40.0 32-33 38.36069778491584 40.0 38.0 40.0 38.0 40.0 34-35 38.33105691841564 40.0 38.0 40.0 38.0 40.0 36-37 38.29197231000454 40.0 38.0 40.0 38.0 40.0 38-39 38.24475949843229 40.0 38.0 40.0 38.0 40.0 40-41 38.203490780587636 40.0 38.0 40.0 38.0 40.0 42-43 38.16786032753186 40.0 38.0 40.0 38.0 40.0 44-45 38.141655963684926 40.0 38.0 40.0 38.0 40.0 46-47 38.106896885947876 40.0 38.0 40.0 38.0 40.0 48-49 38.06273939414937 40.0 38.0 40.0 38.0 40.0 50-51 38.013147798485335 40.0 38.0 40.0 38.0 40.0 52-53 37.970980277642354 40.0 38.0 40.0 38.0 40.0 54-55 37.9223823476279 40.0 38.0 40.0 38.0 40.0 56-57 37.8717688749867 40.0 38.0 40.0 35.0 40.0 58-59 37.84800503844412 40.0 38.0 40.0 32.0 40.0 60-61 37.79027864047798 40.0 38.0 40.0 32.0 40.0 62-63 37.758901952438336 40.0 38.0 40.0 32.0 40.0 64-65 37.719161843107074 40.0 38.0 40.0 32.0 40.0 66-67 37.63293268298799 40.0 38.0 40.0 32.0 40.0 68-69 37.58066670296194 40.0 38.0 40.0 32.0 40.0 70-71 37.562087605245985 40.0 38.0 40.0 32.0 40.0 72-73 37.513625782513756 40.0 38.0 40.0 32.0 40.0 74-75 37.44394099873049 40.0 38.0 40.0 32.0 40.0 76-77 37.37978860476838 40.0 38.0 40.0 32.0 40.0 78-79 37.29481538490727 40.0 38.0 40.0 32.0 40.0 80-81 37.253841566174124 39.0 38.0 40.0 32.0 40.0 82-83 37.181404528165544 38.0 38.0 40.0 32.0 40.0 84-85 37.115110424250524 38.0 38.0 40.0 32.0 40.0 86-87 37.05888091767655 38.0 38.0 40.0 32.0 40.0 88-89 37.01462274773381 38.0 38.0 40.0 32.0 40.0 90-91 36.955553589561475 38.0 38.0 40.0 32.0 40.0 92-93 36.88882625204264 38.0 38.0 40.0 32.0 40.0 94-95 36.80698432456555 38.0 38.0 40.0 32.0 40.0 96-97 36.72989361359885 38.0 38.0 40.0 32.0 40.0 98-99 36.627899559424854 38.0 38.0 40.0 32.0 40.0 100-101 35.81509273030383 38.0 35.0 40.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.0724779854945794 2201 2 0.10799427027570374 2201 3 0.06156627106651058 2201 4 0.05305984696096999 2201 5 0.0966156217995362 2201 6 0.13844195553348015 2201 7 0.20429097375691896 2201 8 0.13879675585648243 2201 9 0.14011207764612976 2201 10-11 0.062096347342944114 2201 12-13 0.06389235194410503 2201 14-15 0.06354062507100622 2201 16-17 0.07707605734729128 2201 18-19 0.04915438069568978 2201 20-21 0.08176252502281045 2201 22-23 0.10159982873089035 2201 24-25 0.028848735402000614 2201 26-27 0.04330468292220502 2201 28-29 0.013160543833791394 2201 30-31 0.030893871622140523 2201 32-33 0.03625867841356012 2201 34-35 0.03412134515323828 2201 36-37 0.05749627493701581 2201 38-39 0.06279279322512821 2201 40-41 0.01686793012212462 2201 42-43 0.008637767145991404 2201 44-45 -0.018512346662078016 2201 46-47 -0.0031294802749641804 2201 48-49 -0.021627875347213887 2201 50-51 -0.04352542693209216 2201 52-53 -0.040335836289429494 2201 54-55 -0.11823534676367586 2201 56-57 -0.1196944253304224 2201 58-59 -0.06872661188243256 2201 60-61 -0.06531464232352846 2201 62-63 -0.11414501865995419 2201 64-65 -0.10188051259388686 2201 66-67 -0.0266602935789777 2201 68-69 -0.032792630712144444 2201 70-71 -0.09120137360051928 2201 72-73 -0.08753559233922203 2201 74-75 -0.07006954369883545 2201 76-77 -0.025375765117509275 2201 78-79 -0.10080846209245209 2201 80-81 -0.11508973298733594 2201 82-83 -0.15710031999030605 2201 84-85 -0.17807448855380414 2201 86-87 -0.283534609690669 2201 88-89 -0.24502684534849806 2201 90-91 -0.23707329705528934 2201 92-93 -0.2776398006556988 2201 94-95 -0.24970465095259442 2201 96-97 -0.23109942276097684 2201 98-99 -0.2841429413411092 2201 100-101 -0.3876936795312602 1101 1 0.139830927187937 1101 2 0.08123286747284197 1101 3 0.09319984312253382 1101 4 0.08249697483728369 1101 5 0.04344666029113142 1101 6 0.1476615462160069 1101 7 0.11168371134361621 1101 8 0.09043365320869157 1101 9 0.10786973478457895 1101 10-11 0.1152717249646571 1101 12-13 0.08220245702096207 1101 14-15 0.05962849200520992 1101 16-17 0.009353114508769522 1101 18-19 0.06568397078909527 1101 20-21 0.028146062370069558 1101 22-23 0.05712719833452695 1101 24-25 0.022722366404885008 1101 26-27 -0.06417417822077454 1101 28-29 0.01212893889081812 1101 30-31 -1.8184095314666138E-4 1101 32-33 0.0018345878736738541 1101 34-35 -0.0032515768156997638 1101 36-37 -0.03056789833961915 1101 38-39 -0.04422087793304996 1101 40-41 0.006325497970124161 1101 42-43 -0.004569447855367059 1101 44-45 -0.020679496792048724 1101 46-47 -0.11542832483151244 1101 48-49 -0.13897027379502447 1101 50-51 -0.09866311601130917 1101 52-53 -0.10954676723762446 1101 54-55 -0.07408924745420364 1101 56-57 -0.09374349915318447 1101 58-59 -0.09469367333784362 1101 60-61 -0.09390668772677202 1101 62-63 -0.08593905822447567 1101 64-65 -0.15659514983903478 1101 66-67 -0.12018255272659673 1101 68-69 -0.1396214972247023 1101 70-71 -0.14369967117331583 1101 72-73 -0.1606251864863708 1101 74-75 -0.17742077524665234 1101 76-77 -0.13318549742649566 1101 78-79 -0.17226807649457498 1101 80-81 -0.17972337070065691 1101 82-83 -0.3075884943849658 1101 84-85 -0.27105551034766506 1101 86-87 -0.21967880202517875 1101 88-89 -0.29312165273614 1101 90-91 -0.28741280800670665 1101 92-93 -0.26825355528905703 1101 94-95 -0.3568445960496618 1101 96-97 -0.327410995144767 1101 98-99 -0.3810221557004283 1101 100-101 -0.3592544690344326 2202 1 0.022461235072142927 2202 2 0.043647566387832626 2202 3 0.045236326256976156 2202 4 -0.013997620034565728 2202 5 0.013009287837888905 2202 6 0.042933083752679124 2202 7 0.0017917658816060111 2202 8 -0.007317781217629715 2202 9 -0.0038769810444279074 2202 10-11 0.028385690960028853 2202 12-13 0.022545869973448873 2202 14-15 -0.03865457330012845 2202 16-17 -0.007557874730792946 2202 18-19 -0.07389588157966642 2202 20-21 -0.11581020516401708 2202 22-23 -0.06209394895917342 2202 24-25 -0.08448831646656885 2202 26-27 -0.08188811472231095 2202 28-29 -0.11861394122400526 2202 30-31 -0.10671810507729873 2202 32-33 -0.12415494411759909 2202 34-35 -0.2040636796178532 2202 36-37 -0.12467647485730993 2202 38-39 -0.12503833614128723 2202 40-41 -0.13265921542556214 2202 42-43 -0.18798412331967285 2202 44-45 -0.22826264336973878 2202 46-47 -0.2471969971789747 2202 48-49 -0.23979162526281073 2202 50-51 -0.24048702622015128 2202 52-53 -0.22163838972056737 2202 54-55 -0.2635250682296473 2202 56-57 -0.3382469733751421 2202 58-59 -0.25019708116366246 2202 60-61 -0.260875714517411 2202 62-63 -0.37216940324188386 2202 64-65 -0.3698434158181314 2202 66-67 -0.28920890735999905 2202 68-69 -0.3323703097847712 2202 70-71 -0.31772831134386337 2202 72-73 -0.37726153915128435 2202 74-75 -0.33783666989242533 2202 76-77 -0.40155920080948704 2202 78-79 -0.40447002885502314 2202 80-81 -0.42662596625858384 2202 82-83 -0.41103558335533563 2202 84-85 -0.46730302685159586 2202 86-87 -0.42478663179219467 2202 88-89 -0.43051181790504955 2202 90-91 -0.4727873836211316 2202 92-93 -0.5045834721933673 2202 94-95 -0.5444969066687264 2202 96-97 -0.5844903938348125 2202 98-99 -0.6523632188361219 2202 100-101 -0.6116891896904448 1102 1 0.08314951125264258 1102 2 0.05167137089947005 1102 3 -0.012322068640507666 1102 4 -0.04223901606746239 1102 5 -0.046865835509223075 1102 6 -0.012987406314699967 1102 7 -0.0389796793284134 1102 8 -0.08091510090841325 1102 9 -0.03537064013314506 1102 10-11 -0.07495776635321505 1102 12-13 -0.08128710685974738 1102 14-15 -0.06502618344843114 1102 16-17 -0.06516895420919866 1102 18-19 -0.10401300314703832 1102 20-21 -0.08093179362067815 1102 22-23 -0.12140409765468974 1102 24-25 -0.11509585853330151 1102 26-27 -0.18908280846548564 1102 28-29 -0.18100599215687652 1102 30-31 -0.16181682622260496 1102 32-33 -0.15466639181038033 1102 34-35 -0.16676816749162526 1102 36-37 -0.20303054934204567 1102 38-39 -0.18120451344743316 1102 40-41 -0.23386147248949385 1102 42-43 -0.25273954920687913 1102 44-45 -0.29365335058647446 1102 46-47 -0.32967230284797466 1102 48-49 -0.3619778325371925 1102 50-51 -0.33648321612213294 1102 52-53 -0.2604028389694264 1102 54-55 -0.3917208383630566 1102 56-57 -0.39041417255849353 1102 58-59 -0.4068662911664518 1102 60-61 -0.37861636452421976 1102 62-63 -0.41792342729600307 1102 64-65 -0.509499041958982 1102 66-67 -0.4101206224937499 1102 68-69 -0.4293530342695462 1102 70-71 -0.5067770513915661 1102 72-73 -0.5191144079741647 1102 74-75 -0.5266380267401445 1102 76-77 -0.5201363193307884 1102 78-79 -0.4929517363839082 1102 80-81 -0.5297502660120941 1102 82-83 -0.6205097307434002 1102 84-85 -0.6020909717351586 1102 86-87 -0.582650945536777 1102 88-89 -0.6533686173300524 1102 90-91 -0.5988213407623206 1102 92-93 -0.6685949980063697 1102 94-95 -0.7486676924769071 1102 96-97 -0.8366368674635396 1102 98-99 -0.9176134875929733 1102 100-101 -0.9298435982068654 2203 1 0.11880832157272181 2203 2 0.04239916181413861 2203 3 0.03698461125613761 2203 4 -0.022080501733867663 2203 5 -0.0017676375237272168 2203 6 -0.10294587289316581 2203 7 8.503000333703881E-4 2203 8 -0.044887983282258404 2203 9 -0.04302679332261761 2203 10-11 -0.06547501959689583 2203 12-13 -0.05951637815221744 2203 14-15 -0.018776035522002132 2203 16-17 -0.13148221310742514 2203 18-19 -0.16620041739481195 2203 20-21 -0.09269198386442667 2203 22-23 -0.07927745606102832 2203 24-25 -0.09717670651330224 2203 26-27 -0.16330478781443958 2203 28-29 -0.1467726648408103 2203 30-31 -0.12869681649876696 2203 32-33 -0.17981636816665514 2203 34-35 -0.21056724070720634 2203 36-37 -0.23960476649889273 2203 38-39 -0.18584465993924937 2203 40-41 -0.18945237116020763 2203 42-43 -0.1955654769544637 2203 44-45 -0.202980772919922 2203 46-47 -0.2710478278058659 2203 48-49 -0.2538708917822845 2203 50-51 -0.3168811991293836 2203 52-53 -0.2335797294959292 2203 54-55 -0.43520115433792483 2203 56-57 -0.32447793398345226 2203 58-59 -0.3201818702102983 2203 60-61 -0.31390986392532483 2203 62-63 -0.3364785137627564 2203 64-65 -0.4161878899072704 2203 66-67 -0.39447934822017316 2203 68-69 -0.38113383912205023 2203 70-71 -0.42788492382132404 2203 72-73 -0.4898230816941833 2203 74-75 -0.48109573211826273 2203 76-77 -0.46109173767831635 2203 78-79 -0.4452528224901613 2203 80-81 -0.4644327240284909 2203 82-83 -0.5474694992971578 2203 84-85 -0.6216500249240511 2203 86-87 -0.5867962761679948 2203 88-89 -0.598728646768798 2203 90-91 -0.6646571541599329 2203 92-93 -0.6962833406998783 2203 94-95 -0.7438890563361085 2203 96-97 -0.7878489377792874 2203 98-99 -0.7475454093663103 2203 100-101 -0.7993741506435512 1103 1 0.08957333174861759 1103 2 0.06189155138186919 1103 3 0.08386330401642539 1103 4 0.09076994601880273 1103 5 0.0667260203342579 1103 6 -0.0014101977153089251 1103 7 0.04695472452456784 1103 8 0.027120262591992628 1103 9 -0.020284348293444054 1103 10-11 0.011976986624482322 1103 12-13 -6.203906118855684E-4 1103 14-15 0.03306192461663926 1103 16-17 -0.0020046776073741057 1103 18-19 0.010901765182545375 1103 20-21 -0.01833402356339775 1103 22-23 0.02144976583412017 1103 24-25 0.031217242817170643 1103 26-27 -0.015101045090922582 1103 28-29 -0.027820654686593116 1103 30-31 -0.014377230738880087 1103 32-33 -0.06758653672912374 1103 34-35 -0.047857621960261554 1103 36-37 -0.06830060433490104 1103 38-39 -0.0074768037067016735 1103 40-41 -0.02698293733042334 1103 42-43 -0.08647927124831511 1103 44-45 -0.09462428801032274 1103 46-47 -0.09674347036740016 1103 48-49 -0.08691456082277682 1103 50-51 -0.12898140309555828 1103 52-53 -0.06331117008359399 1103 54-55 -0.10491318574580788 1103 56-57 -0.07513742581819827 1103 58-59 -0.04904501074699397 1103 60-61 -0.05397337767546162 1103 62-63 -0.11252919626203095 1103 64-65 -0.16045233321913344 1103 66-67 -0.1320705265815576 1103 68-69 -0.15814475030320096 1103 70-71 -0.19224968468643056 1103 72-73 -0.20877177536817726 1103 74-75 -0.25063154851171277 1103 76-77 -0.2056616612039761 1103 78-79 -0.23753179224614485 1103 80-81 -0.23194022961333616 1103 82-83 -0.30655104832818836 1103 84-85 -0.30056197456276834 1103 86-87 -0.2708627112681583 1103 88-89 -0.34329075572121326 1103 90-91 -0.38194911778038687 1103 92-93 -0.293502240333531 1103 94-95 -0.4665771862929091 1103 96-97 -0.4258168687864625 1103 98-99 -0.2957609579981977 1103 100-101 -0.40120359034241204 2204 1 0.1032942714397862 2204 2 0.051133020644968497 2204 3 0.09842867857057058 2204 4 0.0764138540492425 2204 5 0.06374482642283397 2204 6 0.0465802109003306 2204 7 0.10648543360778007 2204 8 0.05800089608067083 2204 9 0.10552159530411842 2204 10-11 0.0690859375730497 2204 12-13 0.1005377106136578 2204 14-15 0.07552858476446289 2204 16-17 0.08330960314391689 2204 18-19 0.05152633292636466 2204 20-21 0.06277763175615547 2204 22-23 0.010407569981595088 2204 24-25 0.024721962746447446 2204 26-27 0.060894877791213275 2204 28-29 0.03800450312494519 2204 30-31 0.026607099756624564 2204 32-33 -0.023915064180179968 2204 34-35 -0.0024800673220894964 2204 36-37 -0.045108534829445546 2204 38-39 -0.007860998833869814 2204 40-41 0.02135379821891803 2204 42-43 -0.03712047063905999 2204 44-45 -0.020412528409728736 2204 46-47 0.025048497752308663 2204 48-49 -0.05521219317049031 2204 50-51 0.0030413720277664424 2204 52-53 0.025236378620014932 2204 54-55 -0.038160697985127 2204 56-57 -0.042599263455237235 2204 58-59 -0.036028346209818096 2204 60-61 -0.026148037264064783 2204 62-63 -3.488378231963907E-5 2204 64-65 -0.005668029666843211 2204 66-67 0.047181962504801334 2204 68-69 0.02834836373072136 2204 70-71 0.052400659120706905 2204 72-73 -0.03953754454430225 2204 74-75 -0.06300958714424354 2204 76-77 -0.029337257428835528 2204 78-79 0.012290834408410944 2204 80-81 0.016987198383475288 2204 82-83 -0.06537451305431574 2204 84-85 -0.05444097210306609 2204 86-87 -0.06550281548397408 2204 88-89 -0.10440235118608854 2204 90-91 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1215 5 -0.038982585336448494 1215 6 -0.007230266045112899 1215 7 -0.08872896672634312 1215 8 -0.07999587790386897 1215 9 -0.05639859370832312 1215 10-11 -0.02552310399254054 1215 12-13 -0.11839498511904623 1215 14-15 -0.07508297951884657 1215 16-17 -0.07737214611299237 1215 18-19 -0.015434749281318716 1215 20-21 -0.029270036720077997 1215 22-23 0.003697823191146199 1215 24-25 -0.002395055952710834 1215 26-27 -0.0065174764350146575 1215 28-29 0.005054242076433013 1215 30-31 -0.0239199327542039 1215 32-33 -4.6431250925138556E-4 1215 34-35 -0.0032393714961713727 1215 36-37 0.01647502913761656 1215 38-39 0.013840767334862392 1215 40-41 0.06417459001683312 1215 42-43 0.06059686127368025 1215 44-45 0.04378569212450856 1215 46-47 0.0633289370455401 1215 48-49 0.10575194617722872 1215 50-51 0.11975990651930601 1215 52-53 0.10965696187044216 1215 54-55 0.19219341482368435 1215 56-57 0.13133839007675618 1215 58-59 0.09896806326455021 1215 60-61 0.21615467751867357 1215 62-63 0.16029318737789566 1215 64-65 0.20749111056534986 1215 66-67 0.2663346268282254 1215 68-69 0.2645183158541613 1215 70-71 0.16612446988862928 1215 72-73 0.172198941769949 1215 74-75 0.20902738166147827 1215 76-77 0.24095570151091295 1215 78-79 0.2574473363945913 1215 80-81 0.3195607730839569 1215 82-83 0.31457356718192386 1215 84-85 0.30625220396876784 1215 86-87 0.41363843792577626 1215 88-89 0.3866148754980472 1215 90-91 0.3955456443430734 1215 92-93 0.40640516740456434 1215 94-95 0.3678260962500346 1215 96-97 0.4413355242587187 1215 98-99 0.5135509145559851 1215 100-101 0.6361742789034608 1212 1 -0.16077451261291742 1212 2 -0.13906251873491726 1212 3 -0.09164650563656807 1212 4 -0.0659939317911693 1212 5 -0.0932078717239051 1212 6 -0.0832707105418109 1212 7 -0.14785919625514765 1212 8 -0.21921592552250502 1212 9 -0.2621428533632866 1212 10-11 -0.24547602290474657 1212 12-13 -0.21764900249939245 1212 14-15 -0.18339250639596827 1212 16-17 -0.06821546224201569 1212 18-19 -0.038428075668861084 1212 20-21 -0.04352933679636806 1212 22-23 -0.019394785639718748 1212 24-25 -0.027695268196303857 1212 26-27 5.786174739341732E-8 1212 28-29 0.02045909607637242 1212 30-31 0.027500679517942217 1212 32-33 -0.05259052305386547 1212 34-35 -0.026338103563332993 1212 36-37 -0.12376812913163349 1212 38-39 -0.14353030156072322 1212 40-41 -0.17769631119973184 1212 42-43 -0.25533717253173194 1212 44-45 -0.07267117861447758 1212 46-47 -0.03730554424033983 1212 48-49 -0.19657603491229025 1212 50-51 -0.10382267460805394 1212 52-53 -0.15205959129468027 1212 54-55 -0.2781280127889332 1212 56-57 -0.16266772980881683 1212 58-59 -0.14792003633911577 1212 60-61 -0.1614569726565307 1212 62-63 -0.13732321877643017 1212 64-65 0.027329096720585255 1212 66-67 0.048170797220173256 1212 68-69 -0.1074826284359105 1212 70-71 0.011335703765489313 1212 72-73 -0.016589407474612017 1212 74-75 0.06635627234885533 1212 76-77 -0.0521192216482973 1212 78-79 0.1786750050213044 1212 80-81 0.21287548417856073 1212 82-83 0.1877895172378885 1212 84-85 0.22844405246000576 1212 86-87 0.20465690782933876 1212 88-89 0.17869115132653945 1212 90-91 0.33705960501534804 1212 92-93 0.3043600565400766 1212 94-95 0.2161026340639438 1212 96-97 0.34630035334564013 1212 98-99 0.312685656523783 1212 100-101 0.3878659572639336 1213 1 -0.30998617346268986 1213 2 -0.17856003233688256 1213 3 -0.12683369893997565 1213 4 -0.1471976960196315 1213 5 -0.11528063549764767 1213 6 -0.01584290443690861 1213 7 -0.03264995557769623 1213 8 -0.06249676452375752 1213 9 -0.019874558441252077 1213 10-11 -0.1075098208765084 1213 12-13 -0.12379841907266353 1213 14-15 -0.2078961893052309 1213 16-17 -0.33543205546984467 1213 18-19 -0.2983074344198329 1213 20-21 -0.12888196976913946 1213 22-23 -0.21808888693369965 1213 24-25 -0.3006135027865895 1213 26-27 -0.10898984001259748 1213 28-29 -0.08741701519847567 1213 30-31 -0.0985866762321237 1213 32-33 -0.12226042638272361 1213 34-35 -0.017918366182250622 1213 36-37 0.030851095607935974 1213 38-39 0.017468099281821026 1213 40-41 0.030675361724817662 1213 42-43 0.08730537248085568 1213 44-45 0.055955584506286016 1213 46-47 0.056955136296224396 1213 48-49 0.14823266057449302 1213 50-51 0.18218342444706082 1213 52-53 0.20294837113052466 1213 54-55 0.1965208049151741 1213 56-57 0.21999788568274425 1213 58-59 0.1920925710897734 1213 60-61 0.22086007390494444 1213 62-63 0.2553499887057953 1213 64-65 0.1963955963159023 1213 66-67 0.27107226447073884 1213 68-69 0.24196134587111118 1213 70-71 0.2234358424982119 1213 72-73 0.14102311623454966 1213 74-75 0.23403718571312027 1213 76-77 0.30722627229165056 1213 78-79 0.3493578263151704 1213 80-81 0.30942275434827593 1213 82-83 0.4146829669198766 1213 84-85 0.33179915016460626 1213 86-87 0.3639521898661613 1213 88-89 0.375084857973512 1213 90-91 0.42901165774804184 1213 92-93 0.4755297902589959 1213 94-95 0.5374386530093389 1213 96-97 0.4703126333656229 1213 98-99 0.5128811738670649 1213 100-101 0.3901436925145987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 12.0 14 32.0 15 90.0 16 176.0 17 353.0 18 827.0 19 1899.0 20 3892.0 21 7448.0 22 12644.0 23 20670.0 24 30759.0 25 43876.0 26 59310.0 27 78668.0 28 99595.0 29 123993.0 30 150904.0 31 182622.0 32 220688.0 33 265664.0 34 329817.0 35 426019.0 36 590666.0 37 941851.0 38 2050379.0 39 6479936.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.930805784564411 33.54403487437339 34.666539731063864 16.858619609998343 2 15.58814516012613 35.08870094597019 33.773185756209024 15.549968137694654 3 15.6497142984412 33.7914209517776 34.03346919314778 16.525395556633416 4 16.03842019865064 33.37761356915364 34.10659592387561 16.477370308320115 5 16.483111560952555 33.56922787576127 33.88287679651301 16.06478376677316 6 16.342046674074204 33.99529316271254 33.03101018824874 16.63164997496451 7 16.30812708955612 33.827905952342654 33.77771948536599 16.086247472735238 8 16.041068103959567 33.987869129136115 33.687599966674334 16.28346280022998 9 15.928596257855665 33.4588463275179 34.11478487363709 16.497772540989352 10-11 16.026702626522678 33.726747026413705 33.87851917598916 16.368031171074453 12-13 15.826756876923548 33.699688768014624 34.098685203653616 16.374869151408216 14-15 15.863258563033813 33.59245918568817 34.08638720857003 16.45789504270798 16-17 15.956265018201254 33.48110459720906 34.13038995148806 16.432240433101622 18-19 16.00707015464262 33.285984496968105 34.082405947805746 16.62453940058353 20-21 16.087326324931027 33.24164783298092 33.912055005196 16.758970836892058 22-23 16.106264791633695 33.289646960738814 33.93871872637066 16.66536952125683 24-25 16.211632917642294 33.157361094481445 33.879293267354335 16.75171272052193 26-27 16.299864831222155 33.16655423224448 33.76510048733682 16.768480449196552 28-29 16.263434601338815 33.175267271123275 33.76896983506425 16.792328292473663 30-31 16.359879881049142 33.03959548327271 33.75070541382477 16.84981922185338 32-33 16.415101320384487 33.17256029184976 33.64092575277498 16.771412634990764 34-35 16.402816248659573 33.134759100395534 33.685002816760125 16.777421834184768 36-37 16.393151658937846 33.02744865241488 33.65401487203825 16.92538481660902 38-39 16.479558454263227 33.04925488496931 33.6772425204083 16.79394414035917 40-41 16.48721812227762 33.12718386193166 33.5597199046769 16.825878111113806 42-43 16.430938619847783 32.97981516938929 33.60741771043996 16.98182850032297 44-45 16.46667071200298 33.00949054874272 33.599229631563226 16.924609107691076 46-47 16.50331128013271 33.0959711263055 33.54726388579224 16.85345370776955 48-49 16.448633514671144 32.92396917566601 33.579782634711044 17.0476146749518 50-51 16.49650105433268 33.00124817083036 33.49677916814517 17.005471606691795 52-53 16.5194326101407 33.06608281259784 33.52098126556772 16.89350331169374 54-55 16.53374257044595 32.921244365402856 33.50697474569364 17.038038318457552 56-57 16.617118327324956 32.95603746607693 33.454123781870486 16.97272042472763 58-59 16.550380258328993 32.98229201084201 33.51354818537351 16.953779545455482 60-61 16.57322737263356 32.93403590476076 33.402579095278625 17.090157627327045 62-63 16.574980754578892 32.89921421267337 33.48329170778901 17.04251332495873 64-65 16.588709196784475 32.93008987034081 33.45625793833367 17.024942994541046 66-67 16.626044429658307 32.9142821647956 33.43493532730615 17.024738078239942 68-69 16.577965979703972 32.895148598793675 33.45547439444875 17.071411027053603 70-71 16.612011160925675 32.940082618039554 33.38757637582068 17.06032984521409 72-73 16.628383844767413 32.869106162867425 33.427062375822494 17.075447616542665 74-75 16.651654614675632 32.90842406288661 33.392725674948345 17.04719564748941 76-77 16.62871089884432 32.95763332595219 33.375086947156056 17.03856882804744 78-79 16.63100096813281 32.88677054171583 33.38522671498681 17.097001775164554 80-81 16.701890765142334 32.899684748718904 33.3803666353423 17.01805785079646 82-83 16.624032679794652 33.0329494527953 33.38630229041093 16.95671557699912 84-85 16.615256476487737 32.83761892748581 33.40624258030921 17.140882015717246 86-87 16.65728137217392 33.0039054402876 33.30660129755083 17.032211889987657 88-89 16.671513289811895 33.001424205991135 33.32325235719126 17.00381014700571 90-91 16.629088555610096 32.84055500855113 33.37368118277687 17.156675253061902 92-93 16.642588474106162 32.874823992282984 33.391712792530654 17.090874741080196 94-95 16.634791497868658 32.93496797316909 33.37302948220739 17.05721104675486 96-97 16.659805122238815 32.896809590141615 33.35043460366568 17.09295068395388 98-99 16.692216366060173 32.87783715610871 33.306869990713835 17.12307648711728 100-101 16.735756744851344 32.910477802579976 33.30258492829084 17.051180524277836 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1357.0 1 1234.5 2 1171.5 3 1386.5 4 1849.0 5 2466.5 6 3228.5 7 4316.5 8 5983.5 9 8344.0 10 11731.0 11 16857.5 12 24853.5 13 36542.5 14 52759.5 15 74796.0 16 103102.5 17 138437.0 18 179015.5 19 223995.0 20 269548.5 21 310916.0 22 351129.5 23 385417.0 24 409675.0 25 424105.0 26 429154.5 27 426243.0 28 423734.5 29 421327.5 30 416551.5 31 414114.0 32 410698.5 33 402057.0 34 389940.5 35 376062.5 36 367618.0 37 365213.5 38 359640.5 39 352254.0 40 339331.5 41 318900.0 42 295460.5 43 271465.5 44 245973.0 45 222029.5 46 202821.0 47 194354.0 48 184579.5 49 164880.5 50 146442.5 51 129572.0 52 113515.0 53 100648.0 54 90567.5 55 81191.5 56 73124.0 57 65990.5 58 58554.5 59 50442.5 60 42024.0 61 33835.0 62 26557.5 63 20294.5 64 15199.0 65 11277.5 66 8124.0 67 5605.5 68 3768.5 69 2585.5 70 1718.0 71 1133.5 72 787.0 73 562.5 74 388.0 75 251.5 76 186.5 77 137.0 78 100.5 79 72.0 80 57.0 81 36.5 82 23.5 83 22.0 84 10.0 85 5.5 86 6.5 87 8.0 88 5.0 89 3.5 90 3.5 91 2.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 1.5 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0961989772981302 2 0.002598411751750216 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 6.599140956825946E-5 10-11 1.0311157745040539E-4 12-13 0.0 14-15 0.001431188695011627 16-17 0.0 18-19 0.0 20-21 6.599140956825946E-5 22-23 1.3610728223453512E-4 24-25 3.670772157234432E-4 26-27 0.0013858196009334486 28-29 0.0018271371524211835 30-31 0.00390174209072334 32-33 0.004334810716015043 34-35 0.005831990820594929 36-37 0.006986840488039469 38-39 0.01132577566715253 40-41 0.012274402179696258 42-43 0.013062174631417355 44-45 0.01514915295901356 46-47 0.014208775372665863 48-49 0.016840182829200207 50-51 0.015285260241248096 52-53 0.016110152860851337 54-55 0.01670820001006369 56-57 0.014835693763564327 58-59 0.011754719829346215 60-61 0.006174321257730275 62-63 0.003514042559509816 64-65 3.9182399431154046E-4 66-67 0.002676776550612524 68-69 0.003856372996645162 70-71 0.004107965245624151 72-73 0.01679481373512203 74-75 0.011696977345973988 76-77 0.03364324549051827 78-79 0.025311830032525514 80-81 0.018972530250874593 82-83 0.030867481825553357 84-85 0.010954573988331068 86-87 0.01548735893305089 88-89 0.007819982033838744 90-91 0.005827866357496913 92-93 0.012517745502479215 94-95 0.012761088825262171 96-97 0.007287926294194653 98-99 0.008170561397170123 100-101 0.0017322745011668105 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.212279E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.75323537601553 #Duplication Level Percentage of deduplicated Percentage of total 1 35.32267735870788 12.275773203582297 2 22.22760479170022 15.44962362342017 3 16.810915145182808 17.52701072780287 4 10.384529286202312 14.435839622100621 5 6.211209311815459 10.792980959161106 6 3.7514481442892635 7.822497621564087 7 1.8292423401385947 4.4500462724627035 8 0.9804584647238913 2.7259283040764974 9 0.6398579314484717 2.0013419968955235 >10 1.7673926873428873 10.754284809505918 >50 0.06944880223647502 1.5290534384158636 >100 0.005215736211809936 0.23561942101242134 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.649785239206486E-5 0.0 0.0 2 1.649785239206486E-5 0.0 3.299570478412972E-5 3 1.649785239206486E-5 0.0 3.299570478412972E-5 4 1.649785239206486E-5 0.0 3.299570478412972E-5 5 1.649785239206486E-5 0.0 4.949355717619459E-5 6 1.649785239206486E-5 0.0 4.949355717619459E-5 7 1.649785239206486E-5 0.0 4.949355717619459E-5 8 2.4746778588097294E-5 8.24892619603243E-6 4.949355717619459E-5 9 2.4746778588097294E-5 5.774248337222702E-5 4.949355717619459E-5 10-11 4.949355717619459E-5 5.774248337222702E-5 6.186694647024324E-5 12-13 7.424033576429188E-5 7.424033576429188E-5 9.073818815635674E-5 14-15 7.83647988623081E-5 7.83647988623081E-5 1.1548496674445404E-4 16-17 9.073818815635674E-5 8.248926196032431E-5 1.1548496674445404E-4 18-19 1.567295977246162E-4 8.248926196032431E-5 1.1960942984247025E-4 20-21 2.10347617998827E-4 9.486265125437297E-5 1.2373389294048647E-4 22-23 2.6809010137105404E-4 1.2373389294048647E-4 1.2785835603850267E-4 24-25 3.62952752625427E-4 1.4023174533255134E-4 1.319828191365189E-4 26-27 4.2894416219368644E-4 1.6497852392064862E-4 1.319828191365189E-4 28-29 5.031844979579783E-4 1.6497852392064862E-4 1.319828191365189E-4 30-31 6.475407063885459E-4 1.6497852392064862E-4 1.319828191365189E-4 32-33 7.918969148191135E-4 1.6497852392064862E-4 1.319828191365189E-4 34-35 9.93995606621908E-4 1.6497852392064862E-4 1.319828191365189E-4 36-37 0.001113605036464378 1.6497852392064862E-4 1.319828191365189E-4 38-39 0.0012538367817969296 1.6497852392064862E-4 1.319828191365189E-4 40-41 0.0014270642319136107 1.6497852392064862E-4 1.319828191365189E-4 42-43 0.0016539097023045026 1.6497852392064862E-4 1.319828191365189E-4 44-45 0.0018065148369311025 1.6497852392064862E-4 1.319828191365189E-4 46-47 0.001967368897753735 1.6497852392064862E-4 1.3610728223453512E-4 48-49 0.0022024632943406595 1.6497852392064862E-4 1.4023174533255134E-4 50-51 0.002449931080221632 1.6497852392064862E-4 1.4023174533255134E-4 52-53 0.0027675147387688807 1.6497852392064862E-4 1.4023174533255134E-4 54-55 0.0031057207128062105 1.814763763127135E-4 1.4023174533255134E-4 56-57 0.0034480511499415563 1.814763763127135E-4 1.4023174533255134E-4 58-59 0.003769759271586821 1.814763763127135E-4 1.6497852392064862E-4 60-61 0.004070845077742005 1.9797422870477835E-4 1.773519132146973E-4 62-63 0.004409051051779335 2.0209869180279458E-4 1.8972530250874593E-4 64-65 0.004718385784130551 2.3096993348890808E-4 1.8972530250874593E-4 66-67 0.005044218368873832 2.3096993348890808E-4 1.8972530250874593E-4 68-69 0.005485535920361566 2.3096993348890808E-4 1.9797422870477835E-4 70-71 0.00585261313608501 2.3096993348890808E-4 2.0622315490081077E-4 72-73 0.006240312667298534 2.3096993348890808E-4 2.1447208109684322E-4 74-75 0.0066898791449823015 2.350943965869243E-4 2.2272100729287564E-4 76-77 0.007180690253646232 2.392188596849405E-4 2.392188596849405E-4 78-79 0.0077539906242704865 2.392188596849405E-4 2.392188596849405E-4 80-81 0.008290170827012593 2.392188596849405E-4 2.7221456446907023E-4 82-83 0.008884093513126929 2.392188596849405E-4 2.969613430571675E-4 84-85 0.009614123481475799 2.392188596849405E-4 3.217081216452648E-4 86-87 0.010570998920215562 2.392188596849405E-4 3.217081216452648E-4 88-89 0.011622737010209695 2.392188596849405E-4 3.217081216452648E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 1210 0.0 16.877066 6 TGCCGTC 1290 0.0 15.462276 5 TATGCCG 1365 0.0 14.26472 3 ATGCCGT 1470 0.0 14.215017 4 CGTATGC 1755 0.0 12.18831 1 GTATGCC 2640 0.0 7.3756614 2 CCGTCTT 3160 0.0 6.6127095 7 >>END_MODULE