##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l02n01 4-59175_2015-03-25 191213422911.341000000044a1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15188209
Sequences flagged as poor quality	0
Sequence length	101
%GC	30
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.94052267782199	38.0	38.0	38.0	32.0	38.0
2	36.947570842618774	38.0	38.0	38.0	32.0	38.0
3	36.92828785803514	38.0	38.0	38.0	32.0	38.0
4	36.91241389949269	38.0	38.0	38.0	32.0	38.0
5	36.88675366529392	38.0	38.0	38.0	32.0	38.0
6	38.793774697200966	40.0	38.0	40.0	38.0	40.0
7	38.74061912105634	40.0	38.0	40.0	38.0	40.0
8	38.724073654767324	40.0	38.0	40.0	38.0	40.0
9	38.719678403161296	40.0	38.0	40.0	38.0	40.0
10-11	38.73407473521071	40.0	38.0	40.0	38.0	40.0
12-13	38.72305003177135	40.0	38.0	40.0	38.0	40.0
14-15	38.70089238303213	40.0	38.0	40.0	38.0	40.0
16-17	38.683894592180025	40.0	38.0	40.0	38.0	40.0
18-19	38.650959668779905	40.0	38.0	40.0	38.0	40.0
20-21	38.629515764498635	40.0	38.0	40.0	38.0	40.0
22-23	38.61371544860886	40.0	38.0	40.0	38.0	40.0
24-25	38.57174539144148	40.0	38.0	40.0	38.0	40.0
26-27	38.50104041233565	40.0	38.0	40.0	38.0	40.0
28-29	38.469949978960656	40.0	38.0	40.0	38.0	40.0
30-31	38.42589702314473	40.0	38.0	40.0	38.0	40.0
32-33	38.396630767985876	40.0	38.0	40.0	38.0	40.0
34-35	38.369076827952526	40.0	38.0	40.0	38.0	40.0
36-37	38.33486206306484	40.0	38.0	40.0	38.0	40.0
38-39	38.29242848844127	40.0	38.0	40.0	38.0	40.0
40-41	38.254308259782306	40.0	38.0	40.0	38.0	40.0
42-43	38.223184774452335	40.0	38.0	40.0	38.0	40.0
44-45	38.19889741443511	40.0	38.0	40.0	38.0	40.0
46-47	38.167500295788656	40.0	38.0	40.0	38.0	40.0
48-49	38.12670585452176	40.0	38.0	40.0	38.0	40.0
50-51	38.08065486852334	40.0	38.0	40.0	38.0	40.0
52-53	38.043663541896215	40.0	38.0	40.0	38.0	40.0
54-55	37.99673286033923	40.0	38.0	40.0	38.0	40.0
56-57	37.94897719671885	40.0	38.0	40.0	38.0	40.0
58-59	37.92769769628532	40.0	38.0	40.0	38.0	40.0
60-61	37.87569719379026	40.0	38.0	40.0	35.0	40.0
62-63	37.84957103895529	40.0	38.0	40.0	32.0	40.0
64-65	37.81392815966649	40.0	38.0	40.0	32.0	40.0
66-67	37.73125099871881	40.0	38.0	40.0	32.0	40.0
68-69	37.68223103198014	40.0	38.0	40.0	32.0	40.0
70-71	37.66755369247289	40.0	38.0	40.0	32.0	40.0
72-73	37.624013963726725	40.0	38.0	40.0	32.0	40.0
74-75	37.557432084322784	40.0	38.0	40.0	32.0	40.0
76-77	37.49551681834244	40.0	38.0	40.0	32.0	40.0
78-79	37.415579414268	40.0	38.0	40.0	32.0	40.0
80-81	37.37765825450519	40.0	38.0	40.0	32.0	40.0
82-83	37.31098176223411	39.0	38.0	40.0	32.0	40.0
84-85	37.249045196836576	38.0	38.0	40.0	32.0	40.0
86-87	37.19737412752221	38.0	38.0	40.0	32.0	40.0
88-89	37.15443555589734	38.0	38.0	40.0	32.0	40.0
90-91	37.09947634378747	38.0	38.0	40.0	32.0	40.0
92-93	37.03620420287869	38.0	38.0	40.0	32.0	40.0
94-95	36.959111209228155	38.0	38.0	40.0	32.0	40.0
96-97	36.88538217376387	38.0	38.0	40.0	32.0	40.0
98-99	36.787451568516076	38.0	38.0	40.0	32.0	40.0
100-101	35.99311936647698	38.0	38.0	40.0	29.5	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.0769325779249499
2201	2	0.1066655466263029
2201	3	0.09267686708759015
2201	4	0.029629162054106928
2201	5	0.0899938153488975
2201	6	0.1891102412030179
2201	7	0.21315028327369134
2201	8	0.16849244660306084
2201	9	0.17182021614092235
2201	10-11	0.09542404182136721
2201	12-13	0.047689271203744
2201	14-15	0.03584002061929681
2201	16-17	0.07068016672920407
2201	18-19	0.065511281920692
2201	20-21	0.12246449135910353
2201	22-23	0.09815739773177512
2201	24-25	0.04346034457745418
2201	26-27	0.05981674999267028
2201	28-29	0.09726256376373499
2201	30-31	0.07858754191713047
2201	32-33	0.05163406998094189
2201	34-35	0.04930490033959245
2201	36-37	0.07241017380867021
2201	38-39	0.05049961363818767
2201	40-41	0.03343795658840065
2201	42-43	-0.016583201678322723
2201	44-45	-0.02163959248154157
2201	46-47	0.017089166568254655
2201	48-49	0.0072663470396534535
2201	50-51	9.677707145101522E-4
2201	52-53	-0.015840258952501074
2201	54-55	-0.10009086730752159
2201	56-57	-0.07411033952456592
2201	58-59	0.004346362611393317
2201	60-61	-0.061741225854369475
2201	62-63	-0.09302437994271173
2201	64-65	-0.12817619662690305
2201	66-67	-0.009341679934209424
2201	68-69	-0.06814327724510605
2201	70-71	-0.03433582011921743
2201	72-73	-0.04385027752081072
2201	74-75	-0.01607656003784541
2201	76-77	-0.05752037052965875
2201	78-79	-0.09771019073578202
2201	80-81	-0.09036088039605517
2201	82-83	-0.1807978745946741
2201	84-85	-0.2107154999425518
2201	86-87	-0.24049642085599032
2201	88-89	-0.23390645316210623
2201	90-91	-0.19352295080066995
2201	92-93	-0.2074694806222368
2201	94-95	-0.2139535464125899
2201	96-97	-0.1919217779735476
2201	98-99	-0.2109635761470372
2201	100-101	-0.3533369345049593
1101	1	0.14036331085496556
1101	2	0.1005726004997669
1101	3	0.07314550391623698
1101	4	-0.02303288313061813
1101	5	0.04305369806853321
1101	6	0.058809124960198744
1101	7	0.09942845817879231
1101	8	0.052200752849579146
1101	9	0.06797642856314923
1101	10-11	0.09536220284373798
1101	12-13	0.06159886433925266
1101	14-15	0.06795583865746124
1101	16-17	0.01478712100326618
1101	18-19	0.04140650554251124
1101	20-21	0.019170265821202292
1101	22-23	0.014342199847980908
1101	24-25	-0.003989666595174413
1101	26-27	-0.047249528031748866
1101	28-29	0.00793912895314719
1101	30-31	0.04000639462997668
1101	32-33	-0.008307000117142138
1101	34-35	-0.003664239585816631
1101	36-37	-0.005731731986962529
1101	38-39	-0.028719139813169647
1101	40-41	-0.0026887558412980184
1101	42-43	8.997864236235387E-4
1101	44-45	-0.07062830775198137
1101	46-47	-0.10471247708142073
1101	48-49	-0.10231693224519489
1101	50-51	-0.08275809464164752
1101	52-53	-0.10529764171295852
1101	54-55	-0.09467483741180871
1101	56-57	-0.07459003353160654
1101	58-59	-0.08424115629619422
1101	60-61	-0.08534144670384336
1101	62-63	-0.08614105878319123
1101	64-65	-0.10788839320683508
1101	66-67	-0.11978040362401288
1101	68-69	-0.0831866527591032
1101	70-71	-0.1135776439050673
1101	72-73	-0.11586309666422068
1101	74-75	-0.12362443276137469
1101	76-77	-0.15090576421007995
1101	78-79	-0.17613789648123657
1101	80-81	-0.20382992517735943
1101	82-83	-0.2829236086137925
1101	84-85	-0.2322340601459345
1101	86-87	-0.23818286795470556
1101	88-89	-0.29558431779916106
1101	90-91	-0.30660085192476316
1101	92-93	-0.345019703477206
1101	94-95	-0.37220904053903325
1101	96-97	-0.33611318711443516
1101	98-99	-0.3848527944892197
1101	100-101	-0.40976851747453225
2202	1	0.07205938620612073
2202	2	0.07251939855567713
2202	3	0.04440701221083998
2202	4	0.0436772755537973
2202	5	0.017998414848719335
2202	6	0.058506107755349035
2202	7	0.03765036779782349
2202	8	0.023026349139151137
2202	9	0.011074589054359762
2202	10-11	0.03740027127069112
2202	12-13	0.03702148806303995
2202	14-15	-0.03591466700175516
2202	16-17	-0.012499625361058975
2202	18-19	-0.024634974599685222
2202	20-21	-0.051801145021499906
2202	22-23	-0.0583839293752888
2202	24-25	-0.0016894872134400885
2202	26-27	-0.00848667161506711
2202	28-29	-9.585930153122035E-5
2202	30-31	-0.020169672127963167
2202	32-33	-0.055550379792371984
2202	34-35	-0.11995348393477201
2202	36-37	-0.08017256704550846
2202	38-39	-0.03011593841130633
2202	40-41	-0.09120175789448837
2202	42-43	-0.07655033047284832
2202	44-45	-0.1479335282298635
2202	46-47	-0.15838552791505833
2202	48-49	-0.17907230547171338
2202	50-51	-0.2195582158979903
2202	52-53	-0.18879314705702654
2202	54-55	-0.11985437795119225
2202	56-57	-0.24806264752045593
2202	58-59	-0.17521345917801767
2202	60-61	-0.17306781465975263
2202	62-63	-0.220390918779664
2202	64-65	-0.24961646150853056
2202	66-67	-0.10133132677611201
2202	68-69	-0.20967883242453667
2202	70-71	-0.2255884646076325
2202	72-73	-0.20825891187161716
2202	74-75	-0.2679594088009267
2202	76-77	-0.2510774445159285
2202	78-79	-0.22805051141427413
2202	80-81	-0.2878220370742284
2202	82-83	-0.3068463572384488
2202	84-85	-0.41989948698522994
2202	86-87	-0.305795300765233
2202	88-89	-0.2988180163993732
2202	90-91	-0.37974312348050177
2202	92-93	-0.38068784586469206
2202	94-95	-0.44830974194864126
2202	96-97	-0.48630290492779693
2202	98-99	-0.5472497267266547
2202	100-101	-0.507695907756343
1102	1	0.08101758842745
1102	2	0.013483126706603343
1102	3	-0.0011762125023295766
1102	4	-0.058432435127741655
1102	5	-0.09601333525472455
1102	6	-0.06648554292127784
1102	7	-0.07936750881410148
1102	8	-0.035146024339383075
1102	9	-0.08197666090843114
1102	10-11	-0.10463808733681645
1102	12-13	-0.10764650153713973
1102	14-15	-0.11536782747527496
1102	16-17	-0.11705615164223815
1102	18-19	-0.1451255590795597
1102	20-21	-0.12771545440573817
1102	22-23	-0.13759617708063843
1102	24-25	-0.11532620860745624
1102	26-27	-0.17186823975932697
1102	28-29	-0.2174559720631919
1102	30-31	-0.2188821386132247
1102	32-33	-0.1747696354519661
1102	34-35	-0.20579484766903988
1102	36-37	-0.23950471952199592
1102	38-39	-0.20212136081324417
1102	40-41	-0.30966328433081003
1102	42-43	-0.311244529250466
1102	44-45	-0.38458154392927923
1102	46-47	-0.4041036117184973
1102	48-49	-0.39196708339036945
1102	50-51	-0.4183533330360376
1102	52-53	-0.38053808098170094
1102	54-55	-0.44319479991929
1102	56-57	-0.48092681845791674
1102	58-59	-0.44407603638162385
1102	60-61	-0.4329984885302878
1102	62-63	-0.44510639189969226
1102	64-65	-0.4824689140324665
1102	66-67	-0.47023239648233783
1102	68-69	-0.42263392581576653
1102	70-71	-0.4935582378561989
1102	72-73	-0.5236705360798979
1102	74-75	-0.5374235172507014
1102	76-77	-0.5025088427445468
1102	78-79	-0.5068517108764894
1102	80-81	-0.5756400443954988
1102	82-83	-0.6312684680981988
1102	84-85	-0.5976367309856983
1102	86-87	-0.6110846755821058
1102	88-89	-0.6406218406875297
1102	90-91	-0.6520715889510598
1102	92-93	-0.7086636744945736
1102	94-95	-0.7799907974799751
1102	96-97	-0.873411656720144
1102	98-99	-0.88424091738122
1102	100-101	-0.9315069897678541
2203	1	0.06854892455133665
2203	2	0.031927231940628076
2203	3	0.0239797762431877
2203	4	-0.058844835238438975
2203	5	0.020307524168018176
2203	6	-0.13724031973427486
2203	7	-0.05000035725911545
2203	8	-0.14795934233617913
2203	9	-0.03545116535372017
2203	10-11	-0.11791465817893965
2203	12-13	-0.06711685041457116
2203	14-15	-0.11334921318346147
2203	16-17	-0.14855127681376246
2203	18-19	-0.17643222787950208
2203	20-21	-0.133854329361057
2203	22-23	-0.10506105727113635
2203	24-25	-0.1301784214509354
2203	26-27	-0.2017679468660205
2203	28-29	-0.17621442589789638
2203	30-31	-0.17615995338704238
2203	32-33	-0.17074167509634464
2203	34-35	-0.2485172202777477
2203	36-37	-0.24339148714459924
2203	38-39	-0.228986912409745
2203	40-41	-0.21760438279600436
2203	42-43	-0.14689045843716286
2203	44-45	-0.23189923514593858
2203	46-47	-0.25212719453004695
2203	48-49	-0.30677664508822744
2203	50-51	-0.3043471667095474
2203	52-53	-0.2527370365403314
2203	54-55	-0.4304533359140308
2203	56-57	-0.33757732698526155
2203	58-59	-0.28471166673643467
2203	60-61	-0.28839042070357834
2203	62-63	-0.3827831512774722
2203	64-65	-0.3911800754005341
2203	66-67	-0.428919198122081
2203	68-69	-0.41078180810495013
2203	70-71	-0.48054589799618697
2203	72-73	-0.47724184522071056
2203	74-75	-0.5274308972393982
2203	76-77	-0.46685085621302136
2203	78-79	-0.4123897509705827
2203	80-81	-0.5093174480808855
2203	82-83	-0.5345656366040998
2203	84-85	-0.5835838703752358
2203	86-87	-0.5417122196602477
2203	88-89	-0.6901406207408058
2203	90-91	-0.692617499111229
2203	92-93	-0.6988801301396848
2203	94-95	-0.6825397763893477
2203	96-97	-0.7502358832627323
2203	98-99	-0.7431913157852961
2203	100-101	-0.8046106209529142
1103	1	0.14701872227519885
1103	2	0.05945461035392441
1103	3	0.055773702683836746
1103	4	0.050358929957788234
1103	5	0.04554046996685912
1103	6	-0.008666690869134186
1103	7	0.007063717925802848
1103	8	-0.04238671368924685
1103	9	-0.06342768419840894
1103	10-11	-0.007108295321835101
1103	12-13	-0.03928135879090178
1103	14-15	-0.025790950558274517
1103	16-17	-0.023281090308145735
1103	18-19	-0.02036746921405097
1103	20-21	-0.023047826901361645
1103	22-23	-0.03520729212640106
1103	24-25	-0.02854175961429206
1103	26-27	-0.012888195843807182
1103	28-29	-0.07831358424304824
1103	30-31	-0.04830414043254194
1103	32-33	-0.134628684881001
1103	34-35	-0.08461573873956496
1103	36-37	-0.1318080093168703
1103	38-39	-0.06158215885007934
1103	40-41	-0.048579292892505066
1103	42-43	-0.11229221351631935
1103	44-45	-0.12161943837526934
1103	46-47	-0.15411853962730504
1103	48-49	-0.14026229972080984
1103	50-51	-0.23785615099430402
1103	52-53	-0.17529203166396456
1103	54-55	-0.21475384856448443
1103	56-57	-0.1399176888511846
1103	58-59	-0.14775081736682694
1103	60-61	-0.14917393679615287
1103	62-63	-0.22824029654415057
1103	64-65	-0.21221495281859148
1103	66-67	-0.18422311238412448
1103	68-69	-0.2042588451384333
1103	70-71	-0.23612398487545505
1103	72-73	-0.2534513145557966
1103	74-75	-0.28742619194561314
1103	76-77	-0.29979019785866257
1103	78-79	-0.2970515871588475
1103	80-81	-0.34508019485563324
1103	82-83	-0.3408784690685138
1103	84-85	-0.32327519761021506
1103	86-87	-0.3538912856113683
1103	88-89	-0.3981062906335495
1103	90-91	-0.3911744511885189
1103	92-93	-0.3819000396428862
1103	94-95	-0.4292013464172655
1103	96-97	-0.4708938405587588
1103	98-99	-0.38252579248671736
1103	100-101	-0.44508205088277464
2204	1	0.09505129097453846
2204	2	0.0720720510876518
2204	3	0.07789671923137576
2204	4	0.10823146778810155
2204	5	0.07725484100272695
2204	6	0.060550535436725283
2204	7	0.10993072307866925
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1212	96-97	0.34695743537608337
1212	98-99	0.3537883651799092
1212	100-101	0.40642717239533965
1213	1	-0.37912443668317053
1213	2	-0.19533907737231715
1213	3	-0.1591063187472912
1213	4	-0.1574720714024096
1213	5	-0.12191923264089866
1213	6	-0.02361264901708182
1213	7	-0.05049143912526688
1213	8	-0.09068676974436585
1213	9	-0.049152281686268395
1213	10-11	-0.09675195288314598
1213	12-13	-0.12058372827819142
1213	14-15	-0.20687139151512923
1213	16-17	-0.3034832886156593
1213	18-19	-0.27676165594625957
1213	20-21	-0.14250723068727922
1213	22-23	-0.2263440256817688
1213	24-25	-0.291168827413415
1213	26-27	-0.13174823895923993
1213	28-29	-0.13283611528578376
1213	30-31	-0.11881441665668291
1213	32-33	-0.1511472907480922
1213	34-35	-0.03676347771360611
1213	36-37	0.008231636472935122
1213	38-39	0.012806870255701597
1213	40-41	0.04705631274213573
1213	42-43	0.06412376430625244
1213	44-45	0.050953621052038045
1213	46-47	0.10613433551350226
1213	48-49	0.11054568690799016
1213	50-51	0.14572596064147803
1213	52-53	0.15450372185909345
1213	54-55	0.15902018551352626
1213	56-57	0.16444396163438313
1213	58-59	0.15807297480611737
1213	60-61	0.16728262063447374
1213	62-63	0.20599426892896844
1213	64-65	0.20554945255225476
1213	66-67	0.26374576787056014
1213	68-69	0.22743352872684142
1213	70-71	0.23293836199639628
1213	72-73	0.1783330482933465
1213	74-75	0.2067430374829513
1213	76-77	0.2713273701814529
1213	78-79	0.3130535810448123
1213	80-81	0.26851851358540557
1213	82-83	0.3739646105444834
1213	84-85	0.2916406385898256
1213	86-87	0.3114085498213086
1213	88-89	0.32794065744140255
1213	90-91	0.3984306351049227
1213	92-93	0.41715369646255596
1213	94-95	0.47295315997859433
1213	96-97	0.4254764229173489
1213	98-99	0.4542382712912101
1213	100-101	0.3647384060762917
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	14.0
14	35.0
15	102.0
16	207.0
17	401.0
18	975.0
19	1998.0
20	3881.0
21	7331.0
22	13197.0
23	21687.0
24	33159.0
25	48210.0
26	67524.0
27	90540.0
28	116812.0
29	147292.0
30	181404.0
31	220896.0
32	267778.0
33	324907.0
34	405612.0
35	524235.0
36	731331.0
37	1174856.0
38	2553407.0
39	8250418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.241838537937333	35.33469466165763	36.21216819269661	15.211298607708432
2	13.875405851851696	36.947161828751064	35.39524597624621	13.782186343151032
3	13.790263223267472	35.58105501445233	35.703775211415646	14.924906550864556
4	14.396621747830835	35.15766737210424	35.7299599972584	14.715750882806525
5	14.866664002319169	35.39503571487593	35.419995866530414	14.318304416274493
6	14.651246898169495	35.735938318994684	34.65203171749875	14.960783065337067
7	14.688374383049377	35.466110586179056	35.32527107047315	14.520243960298412
8	14.44855677190115	35.77917580670638	35.12344345538042	14.648823966012056
9	14.378272976503274	35.15925948043485	35.56895250464489	14.893515038416993
10-11	14.41237279225351	35.53084791864525	35.36475183212174	14.692027456979503
12-13	14.241692354905044	35.418125994974126	35.565269743127715	14.774911906993115
14-15	14.285264682333365	35.295517553524775	35.531549452282675	14.887668311859183
16-17	14.375302578467283	35.189544073300546	35.62906264984897	14.806090698383201
18-19	14.465675314317835	35.042383865010024	35.46221940980664	15.029721410865493
20-21	14.557370862319399	34.93714095867519	35.37468160292185	15.130806576083561
22-23	14.544106368459461	35.00566377690977	35.351030881797506	15.09919897283326
24-25	14.650452054652465	34.9058188611156	35.25039664962543	15.193332434606512
26-27	14.707828741224164	34.9318771847003	35.203038449408446	15.15725562466709
28-29	14.74427797822704	34.882908781764826	35.153995105269686	15.218818134738447
30-31	14.73995956058265	34.806698623757434	35.14896669051718	15.30437512514274
32-33	14.828824535379281	34.89437404967723	35.05308573051323	15.223715684430255
34-35	14.814518149761296	34.92905295483277	35.05531594274011	15.201112952665833
36-37	14.820378478030419	34.7480285908444	35.03296318645815	15.398629744667037
38-39	14.92760635986367	34.846886092176284	34.98320105648954	15.242306491470512
40-41	14.915509092923454	34.89669620461337	34.940715861019704	15.24707884144347
42-43	14.839827537654651	34.696393383985004	35.009142961786246	15.454636116574106
44-45	14.929067283165312	34.795048233812835	34.96999912847219	15.305885354549664
46-47	14.985865373028378	34.870974199449364	34.90693469229447	15.236225735227785
48-49	14.914963710156037	34.66488999782526	34.964556345606866	15.455589946411836
50-51	15.008897369535484	34.68752265663476	34.89469443101646	15.408885542813294
52-53	14.978398103572877	34.823300118903695	34.90508036131998	15.293221416203453
54-55	14.960103889477727	34.701580662428114	34.900227374295255	15.438088073798905
56-57	15.055864626857534	34.689627865040926	34.846259013896756	15.408248494204793
58-59	15.045837638834403	34.7149710276886	34.93379391837445	15.305397415102542
60-61	15.059638071113335	34.69659773098223	34.80900399777565	15.434760200128785
62-63	15.04081869350881	34.640375848617865	34.85840410244849	15.460401355424835
64-65	15.007264216809055	34.75482330037869	34.86761190370281	15.370300579109447
66-67	15.092541314691827	34.68967800841165	34.825330033921404	15.392450642975128
68-69	15.110039740304002	34.702442202206484	34.806122150669715	15.381395906819797
70-71	15.086562038819165	34.71599532130721	34.828221923900884	15.369220715972737
72-73	15.080750375817454	34.628032254844136	34.80634963574616	15.484867733592244
74-75	15.119902225640194	34.7155211857561	34.78650248418083	15.378074104422875
76-77	15.102318463717573	34.7183242586337	34.82483179255486	15.354525485093868
78-79	15.128781976516365	34.6267663932024	34.79739123390843	15.447060396372805
80-81	15.165079278738094	34.682677279794255	34.80892175476692	15.343321686700731
82-83	15.124809618446552	34.79655806616798	34.749285811902205	15.329346503483263
84-85	15.069641160734083	34.6016423936718	34.813774977540895	15.514941468053223
86-87	15.15662950424262	34.75946439038849	34.744779546079606	15.339126559289284
88-89	15.157877859501667	34.796841570351965	34.757811421001975	15.287469149144389
90-91	15.107753273658867	34.63700365726916	34.776623713200145	15.478619355871832
92-93	15.173733750571078	34.66398761614637	34.79181726914903	15.370461364133526
94-95	15.150137605176067	34.77252245163076	34.777003493039274	15.300336450153896
96-97	15.130078294128216	34.66115634946709	34.81208780402142	15.396677552383279
98-99	15.199493220947838	34.667693253548315	34.74765035076789	15.385163174735956
100-101	15.177182727847317	34.777407919521465	34.78611218807962	15.259297164551597
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1482.0
1	1367.5
2	1359.5
3	1664.5
4	2153.5
5	2804.5
6	3694.5
7	4898.5
8	6550.0
9	9065.0
10	12687.0
11	18282.5
12	27197.0
13	40813.0
14	61338.5
15	90768.0
16	130748.5
17	181956.0
18	243304.0
19	313628.5
20	390328.5
21	467533.0
22	543158.5
23	610995.0
24	665687.0
25	704938.5
26	725692.5
27	734872.0
28	741314.5
29	744574.0
30	738861.0
31	726958.5
32	706330.0
33	666729.0
34	606904.5
35	532576.5
36	456116.0
37	388826.5
38	332289.0
39	287358.5
40	252576.5
41	226640.5
42	212547.0
43	204791.5
44	191702.0
45	173422.5
46	157352.5
47	151626.5
48	139756.0
49	112609.5
50	84987.0
51	63585.5
52	49743.0
53	40043.5
54	33373.0
55	27811.5
56	23657.0
57	20452.5
58	18021.0
59	15691.5
60	12936.5
61	10468.0
62	8438.0
63	6786.5
64	5323.5
65	4115.5
66	3172.0
67	2444.0
68	1895.0
69	1422.5
70	1034.5
71	760.5
72	547.0
73	400.0
74	276.5
75	214.5
76	166.0
77	112.0
78	89.0
79	59.5
80	32.5
81	21.0
82	14.0
83	8.5
84	4.0
85	2.5
86	2.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	1.0
97	1.0
98	0.0
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09417831951087846
2	0.002640205965035114
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	5.2672438205189305E-5
10-11	8.230068469560828E-5
12-13	0.0
14-15	0.0014452000232548815
16-17	0.0
18-19	0.0
20-21	1.3168109551297324E-4
22-23	1.4814123245209492E-4
24-25	3.7199909482414943E-4
26-27	0.0013102269003540839
28-29	0.0015966332830948005
30-31	0.003634398236158062
32-33	0.00398993719404309
34-35	0.005665579134445675
36-37	0.0068441249392867855
38-39	0.011117176488682767
40-41	0.011927015226087552
42-43	0.012756606127819285
44-45	0.014705486341411288
46-47	0.013819930974086542
48-49	0.0163712521996504
50-51	0.014879963792965977
52-53	0.01573259888641248
54-55	0.01616714650160529
56-57	0.014570513218510491
58-59	0.011367370570157416
60-61	0.005849932668163837
62-63	0.0032821513056608582
64-65	4.0162734131456845E-4
66-67	0.0029759927585931954
68-69	0.003983353139267441
70-71	0.004170998700373428
72-73	0.017414824881590713
74-75	0.011946767390414498
76-77	0.03453665932566506
78-79	0.026461316143331977
80-81	0.0196863237791895
82-83	0.03215652352426807
84-85	0.011298237995013104
86-87	0.0163580840900991
88-89	0.008108263456211328
90-91	0.006063914448372418
92-93	0.0130561806201113
94-95	0.013214197934726866
96-97	0.007785644772204544
98-99	0.008654740002590167
100-101	0.0017019781595051793
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.5188209E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.63654042092463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.84390832227896	40.95405153494664
2	18.130584659343395	21.2622952046517
3	6.1657411664552	10.846131933954279
4	2.6933440646832727	6.317135124650326
5	1.1415124009398	3.3467169019346654
6	0.5858184712190168	2.061022108017489
7	0.33179767486862066	1.3618827441801877
8	0.20780883089301488	0.9748152729986687
9	0.11607199444069943	0.612545417438347
>10	0.6910682058734009	7.517007165324145
>50	0.06766933829709085	2.6674106264962756
>100	0.02467487070762267	2.078985965407316
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.975216432694599E-5	0.0	6.584054775648663E-6
2	1.975216432694599E-5	0.0	6.584054775648663E-6
3	1.975216432694599E-5	6.584054775648663E-6	1.3168109551297326E-5
4	2.6336219102594652E-5	6.584054775648663E-6	1.975216432694599E-5
5	2.6336219102594652E-5	6.584054775648663E-6	2.6336219102594652E-5
6	2.6336219102594652E-5	6.584054775648663E-6	3.2920273878243316E-5
7	2.6336219102594652E-5	1.3168109551297326E-5	5.9256492980837965E-5
8	2.6336219102594652E-5	1.3168109551297326E-5	5.9256492980837965E-5
9	2.6336219102594652E-5	1.3168109551297326E-5	7.242460253213529E-5
10-11	2.6336219102594652E-5	1.3168109551297326E-5	7.571662991995962E-5
12-13	3.950432865389198E-5	1.3168109551297326E-5	8.559271208343261E-5
14-15	5.925649298083797E-5	1.3168109551297326E-5	8.888473947125694E-5
16-17	7.571662991995962E-5	1.975216432694599E-5	1.1192893118602727E-4
18-19	8.888473947125694E-5	2.304419171477032E-5	1.3168109551297326E-4
20-21	1.3826515028862193E-4	2.6336219102594652E-5	1.4814123245209492E-4
22-23	1.8435353371816255E-4	3.6212301266067646E-5	1.6460136939121657E-4
24-25	2.2714988975987887E-4	4.279635604171631E-5	1.9752164326945987E-4
26-27	2.7653030057724385E-4	4.608838342954064E-5	2.1068975282075722E-4
28-29	3.7529112221197376E-4	4.608838342954064E-5	2.304419171477032E-4
30-31	4.6417586168323073E-4	4.608838342954064E-5	2.4361002669900055E-4
32-33	5.003881629492984E-4	4.938041081736497E-5	2.699462458015952E-4
34-35	6.090250667475013E-4	5.2672438205189305E-5	2.798223279650682E-4
36-37	7.110779157700556E-4	5.2672438205189305E-5	3.061585470676628E-4
38-39	8.361749565073801E-4	5.2672438205189305E-5	3.1932665661896014E-4
40-41	9.217676685908128E-4	5.9256492980837965E-5	3.5224693049720345E-4
42-43	0.0010402806545524888	6.584054775648663E-5	3.555389578850278E-4
44-45	0.0011851298596167593	7.242460253213529E-5	3.555389578850278E-4
46-47	0.0013299790646810299	7.242460253213529E-5	3.555389578850278E-4
48-49	0.0015077485436235437	7.571662991995962E-5	3.8516720437544675E-4
50-51	0.0017348984333834228	8.559271208343261E-5	4.082113960902171E-4
52-53	0.001899499802774639	8.559271208343261E-5	4.2467153302933877E-4
54-55	0.00206739319955368	8.559271208343261E-5	4.378396425806361E-4
56-57	0.002212242404617951	9.217676685908127E-5	4.6417586168323073E-4
58-59	0.0024426843217656537	1.0534487641037861E-4	4.806359986223524E-4
60-61	0.0026599581293620597	1.0534487641037861E-4	4.938041081736498E-4
62-63	0.0029430724847149523	1.0534487641037861E-4	5.036801903371227E-4
64-65	0.0031899745388017774	1.250970407373246E-4	5.168482998884201E-4
66-67	0.003509301195420737	1.3168109551297326E-4	5.267243820518931E-4
68-69	0.003822043797264049	1.3168109551297326E-4	5.36600464215366E-4
70-71	0.004203918974251672	1.3168109551297326E-4	5.596446559301364E-4
72-73	0.004569334014300172	1.4484920506427059E-4	5.892729024205553E-4
74-75	0.0050894743415764165	1.4484920506427059E-4	5.95856957196204E-4
76-77	0.0056063226414648365	1.4484920506427059E-4	6.05733039359677E-4
78-79	0.0062778962285809994	1.4484920506427059E-4	6.25485203686623E-4
80-81	0.007041646582556245	1.4484920506427059E-4	6.35361285850096E-4
82-83	0.008055591018006139	1.4484920506427059E-4	6.518214227892176E-4
84-85	0.009224260740683776	1.4484920506427059E-4	6.715735871161636E-4
86-87	0.010705673065204726	1.5143325983991925E-4	7.044938609944069E-4
88-89	0.01284549086729054	1.5472528722774358E-4	7.209539979335285E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	1220	0.0	21.799492	6
TGCCGTC	1415	0.0	17.78843	5
ATGCCGT	1715	0.0	15.784429	4
TATGCCG	1780	0.0	14.941223	3
CGTATGC	2165	0.0	11.637303	1
GTATGCC	3265	0.0	8.582195	2
CCGTCTT	3975	0.0	7.885422	7
>>END_MODULE