##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n01 3-58925.34100000001ae7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13790623 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.92743750590528 38.0 38.0 38.0 32.0 38.0 2 36.93408310850061 38.0 38.0 38.0 32.0 38.0 3 36.916391521978376 38.0 38.0 38.0 32.0 38.0 4 36.903937697375966 38.0 38.0 38.0 32.0 38.0 5 36.88322035922525 38.0 38.0 38.0 32.0 38.0 6 38.79822738972706 40.0 38.0 40.0 38.0 40.0 7 38.74590103724828 40.0 38.0 40.0 38.0 40.0 8 38.73298624724931 40.0 38.0 40.0 38.0 40.0 9 38.729091716886174 40.0 38.0 40.0 38.0 40.0 10-11 38.74297669510652 40.0 38.0 40.0 38.0 40.0 12-13 38.73403667114967 40.0 38.0 40.0 38.0 40.0 14-15 38.7139711164608 40.0 38.0 40.0 38.0 40.0 16-17 38.69590220833388 40.0 38.0 40.0 38.0 40.0 18-19 38.66451660668267 40.0 38.0 40.0 38.0 40.0 20-21 38.64198919802246 40.0 38.0 40.0 38.0 40.0 22-23 38.626259089237664 40.0 38.0 40.0 38.0 40.0 24-25 38.58243061245311 40.0 38.0 40.0 38.0 40.0 26-27 38.51387598660337 40.0 38.0 40.0 38.0 40.0 28-29 38.47944940558523 40.0 38.0 40.0 38.0 40.0 30-31 38.435849888725116 40.0 38.0 40.0 38.0 40.0 32-33 38.404600756615565 40.0 38.0 40.0 38.0 40.0 34-35 38.37635910284836 40.0 38.0 40.0 38.0 40.0 36-37 38.3420235619522 40.0 38.0 40.0 38.0 40.0 38-39 38.29478798746075 40.0 38.0 40.0 38.0 40.0 40-41 38.257857023573195 40.0 38.0 40.0 38.0 40.0 42-43 38.22410785937662 40.0 38.0 40.0 38.0 40.0 44-45 38.200401062374056 40.0 38.0 40.0 38.0 40.0 46-47 38.167067180358714 40.0 38.0 40.0 38.0 40.0 48-49 38.12659569477028 40.0 38.0 40.0 38.0 40.0 50-51 38.07798744842782 40.0 38.0 40.0 38.0 40.0 52-53 38.03956412991639 40.0 38.0 40.0 38.0 40.0 54-55 37.99138450815456 40.0 38.0 40.0 38.0 40.0 56-57 37.94221363313318 40.0 38.0 40.0 38.0 40.0 58-59 37.921231658642256 40.0 38.0 40.0 38.0 40.0 60-61 37.866611392393224 40.0 38.0 40.0 35.0 40.0 62-63 37.837402958517536 40.0 38.0 40.0 32.0 40.0 64-65 37.79955372574538 40.0 38.0 40.0 32.0 40.0 66-67 37.71352171689415 40.0 38.0 40.0 32.0 40.0 68-69 37.66303375126708 40.0 38.0 40.0 32.0 40.0 70-71 37.64689572037463 40.0 38.0 40.0 32.0 40.0 72-73 37.60187483915701 40.0 38.0 40.0 32.0 40.0 74-75 37.5334708954048 40.0 38.0 40.0 32.0 40.0 76-77 37.46871355993127 40.0 38.0 40.0 32.0 40.0 78-79 37.3885489074714 40.0 38.0 40.0 32.0 40.0 80-81 37.34926319137286 40.0 38.0 40.0 32.0 40.0 82-83 37.279400285251796 38.0 38.0 40.0 32.0 40.0 84-85 37.2167201945844 38.0 38.0 40.0 32.0 40.0 86-87 37.160628783775756 38.0 38.0 40.0 32.0 40.0 88-89 37.11550402762805 38.0 38.0 40.0 32.0 40.0 90-91 37.05873034162416 38.0 38.0 40.0 32.0 40.0 92-93 36.994197869088296 38.0 38.0 40.0 32.0 40.0 94-95 36.9172216512626 38.0 38.0 40.0 32.0 40.0 96-97 36.83952675669548 38.0 38.0 40.0 32.0 40.0 98-99 36.74050555946602 38.0 38.0 40.0 32.0 40.0 100-101 35.921075393040624 38.0 35.0 40.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.07641913375321252 2201 2 0.06659272076332456 2201 3 0.049026254064543195 2201 4 -0.010446770419939355 2201 5 0.08086013701062456 2201 6 0.13507758896619748 2201 7 0.20262422634711896 2201 8 0.09656403415736747 2201 9 0.1518221390899015 2201 10-11 0.10550557619951206 2201 12-13 0.0541340841219764 2201 14-15 0.038396910316514266 2201 16-17 0.06482706039389541 2201 18-19 0.04528951789055213 2201 20-21 0.09566059644154734 2201 22-23 0.0935526663432853 2201 24-25 0.031810141914824897 2201 26-27 0.05344198449876103 2201 28-29 0.051648576184653905 2201 30-31 0.015061450492012796 2201 32-33 0.009555961325041551 2201 34-35 -0.006705390167155656 2201 36-37 0.0411715964545607 2201 38-39 0.006552105911517003 2201 40-41 -0.012873639484865862 2201 42-43 -0.06157980984184519 2201 44-45 -0.057391352350940394 2201 46-47 -0.040324807401269425 2201 48-49 -0.07376935278017527 2201 50-51 -0.07216961622470563 2201 52-53 -0.08288209639410837 2201 54-55 -0.17684973022680595 2201 56-57 -0.16808612967219005 2201 58-59 -0.110842041999895 2201 60-61 -0.07473921170084452 2201 62-63 -0.12676134596274835 2201 64-65 -0.1586376024574463 2201 66-67 -0.05298699769958404 2201 68-69 -0.11227604306426997 2201 70-71 -0.07704925459680823 2201 72-73 -0.08711069927161219 2201 74-75 -0.09292938767743664 2201 76-77 -0.10476319091258546 2201 78-79 -0.08032566939937169 2201 80-81 -0.12228988093793447 2201 82-83 -0.2047169439537342 2201 84-85 -0.24159259070447092 2201 86-87 -0.2720212387331742 2201 88-89 -0.3079372435116028 2201 90-91 -0.24286679457448201 2201 92-93 -0.2552155794112849 2201 94-95 -0.3098460619429986 2201 96-97 -0.23140449899181448 2201 98-99 -0.33286440374133264 2201 100-101 -0.43700957640523086 1101 1 0.0937674557080399 1101 2 0.0777898847516525 1101 3 0.09742247100221846 1101 4 0.01729009612784438 1101 5 0.044658196215266344 1101 6 0.11960763791991269 1101 7 0.12139810510598892 1101 8 0.09513756748531677 1101 9 0.06197842345910942 1101 10-11 0.06780520473886753 1101 12-13 0.05791253145004305 1101 14-15 0.043301216121463426 1101 16-17 0.011261264227030665 1101 18-19 0.03739889865784107 1101 20-21 0.015766085787788597 1101 22-23 0.03713302227426851 1101 24-25 -0.006769602903780481 1101 26-27 -0.03565366016945859 1101 28-29 -0.004387910272804163 1101 30-31 -0.0010630614740207989 1101 32-33 -0.04475975368065832 1101 34-35 0.010561085877483833 1101 36-37 -0.023600213808506965 1101 38-39 -0.06313258355194051 1101 40-41 -0.04297957250901874 1101 42-43 -0.02361900038950182 1101 44-45 -0.06146213464138128 1101 46-47 -0.09883637402795387 1101 48-49 -0.12361570702818625 1101 50-51 -0.06783505663318579 1101 52-53 -0.07315142389003171 1101 54-55 -0.07734301000737531 1101 56-57 -0.08462972496590027 1101 58-59 -0.10575039249643936 1101 60-61 -0.10876152147676521 1101 62-63 -0.1021645136353797 1101 64-65 -0.14584617619056672 1101 66-67 -0.09898898608948059 1101 68-69 -0.1492324614185776 1101 70-71 -0.16112675208834304 1101 72-73 -0.1543716785878786 1101 74-75 -0.19919529219362886 1101 76-77 -0.1895760821851411 1101 78-79 -0.2194254788242418 1101 80-81 -0.1906340487356033 1101 82-83 -0.3309306680087758 1101 84-85 -0.2473293366243965 1101 86-87 -0.2378690710945932 1101 88-89 -0.33827231319202156 1101 90-91 -0.29194717597238906 1101 92-93 -0.31164903096180296 1101 94-95 -0.35895531054492125 1101 96-97 -0.3549226358973101 1101 98-99 -0.36144177727507554 1101 100-101 -0.3658045115733799 2202 1 0.08842212346245759 2202 2 0.09150646871528778 2202 3 0.0826562130944879 2202 4 0.07704731761972283 2202 5 0.08231952102953954 2202 6 0.08458212831130396 2202 7 0.1098517341951677 2202 8 0.05446124210119763 2202 9 0.05247901154774581 2202 10-11 0.09549927345807419 2202 12-13 0.057128227190226255 2202 14-15 0.019860026602195546 2202 16-17 0.032104545244962424 2202 18-19 -0.006397259683275536 2202 20-21 0.003796175832050608 2202 22-23 0.0011349946182406256 2202 24-25 -0.013327697876313493 2202 26-27 0.005585081232936773 2202 28-29 -0.0020666331858194553 2202 30-31 -0.00469517269301889 2202 32-33 -0.05035080219256116 2202 34-35 -0.11871090443592891 2202 36-37 -0.07012115411273356 2202 38-39 -0.024850543849474604 2202 40-41 -0.017707623723232757 2202 42-43 -0.0728238594449806 2202 44-45 -0.12617229202229652 2202 46-47 -0.12277200719709214 2202 48-49 -0.12769725745095428 2202 50-51 -0.14942703585184347 2202 52-53 -0.1334124803802368 2202 54-55 -0.13272879012784244 2202 56-57 -0.1895320944126695 2202 58-59 -0.1631287464403428 2202 60-61 -0.17728983464634496 2202 62-63 -0.18927996188388363 2202 64-65 -0.23295693104024906 2202 66-67 -0.09142552686243732 2202 68-69 -0.14100046135697397 2202 70-71 -0.14740427027899017 2202 72-73 -0.18971921239246115 2202 74-75 -0.21553267976799617 2202 76-77 -0.21105100761243278 2202 78-79 -0.14461747842808848 2202 80-81 -0.2770487212675192 2202 82-83 -0.23166205639642357 2202 84-85 -0.27456246321700206 2202 86-87 -0.25230364496027846 2202 88-89 -0.2298003712312351 2202 90-91 -0.36145890940572656 2202 92-93 -0.35371329024799536 2202 94-95 -0.35945747843099696 2202 96-97 -0.4045031064166338 2202 98-99 -0.4428795478520158 2202 100-101 -0.5140956769178544 1102 1 0.09046189426364037 1102 2 0.03934496047025959 1102 3 0.0037284093685983066 1102 4 -0.04763813376332848 1102 5 -0.059982492954965494 1102 6 -0.05642210778634649 1102 7 -0.06181173568532472 1102 8 -0.05024638990839492 1102 9 -0.051601510424973185 1102 10-11 -0.0646657928476273 1102 12-13 -0.0763245056206685 1102 14-15 -0.08504421900843084 1102 16-17 -0.06876505574211222 1102 18-19 -0.14254378892758268 1102 20-21 -0.10678466530204389 1102 22-23 -0.10123762142977455 1102 24-25 -0.11420800624363636 1102 26-27 -0.1696641526617384 1102 28-29 -0.18277575408605884 1102 30-31 -0.17273159182854414 1102 32-33 -0.13426614495944023 1102 34-35 -0.2009056481871241 1102 36-37 -0.2451833388138951 1102 38-39 -0.20408557031689156 1102 40-41 -0.2699369109932164 1102 42-43 -0.29420097349042607 1102 44-45 -0.35179381516693553 1102 46-47 -0.3529461006388459 1102 48-49 -0.3364959255931552 1102 50-51 -0.3111042089793159 1102 52-53 -0.30293615522733575 1102 54-55 -0.3383514388256259 1102 56-57 -0.4188891035651494 1102 58-59 -0.3896726768031087 1102 60-61 -0.4047344334510683 1102 62-63 -0.40043778961409515 1102 64-65 -0.4793020208927885 1102 66-67 -0.4201832238472747 1102 68-69 -0.4392736046771688 1102 70-71 -0.48367077925644963 1102 72-73 -0.4820680189960811 1102 74-75 -0.48949764574299337 1102 76-77 -0.4886052943424417 1102 78-79 -0.5076177059346492 1102 80-81 -0.520383277477606 1102 82-83 -0.6241915561356706 1102 84-85 -0.5793890657549596 1102 86-87 -0.5788314854182488 1102 88-89 -0.5910369398229989 1102 90-91 -0.6774448762216139 1102 92-93 -0.693364894983759 1102 94-95 -0.6736810021340034 1102 96-97 -0.7716583904989847 1102 98-99 -0.9057953567912307 1102 100-101 -0.8977642963169785 2203 1 0.09149542666761334 2203 2 0.011751900933866466 2203 3 0.0028308589241632376 2203 4 -0.01920629579363009 2203 5 -0.05237634856537454 2203 6 -0.13616357975378435 2203 7 -0.048742609477770316 2203 8 -0.10539361727186503 2203 9 -0.059108896287277446 2203 10-11 -0.10884876440291436 2203 12-13 -0.07059249674640711 2203 14-15 -0.0895216835220154 2203 16-17 -0.1283019545380597 2203 18-19 -0.1337404211349451 2203 20-21 -0.12072789049661026 2203 22-23 -0.06970730635027422 2203 24-25 -0.12667967203463348 2203 26-27 -0.20285711438148013 2203 28-29 -0.15249567365147243 2203 30-31 -0.13434469272500138 2203 32-33 -0.15590262988713022 2203 34-35 -0.18306081818299447 2203 36-37 -0.21864593373060615 2203 38-39 -0.18333404555509247 2203 40-41 -0.19870281797168587 2203 42-43 -0.18432292767265324 2203 44-45 -0.24640998614503928 2203 46-47 -0.26822284052236967 2203 48-49 -0.27958522509369743 2203 50-51 -0.29517920095334205 2203 52-53 -0.24456997855797624 2203 54-55 -0.43917192182033915 2203 56-57 -0.3183381950301367 2203 58-59 -0.3024078052512351 2203 60-61 -0.28809956136499437 2203 62-63 -0.3767102062618477 2203 64-65 -0.3287196392680869 2203 66-67 -0.35632533971406133 2203 68-69 -0.416010497467326 2203 70-71 -0.5088549944080185 2203 72-73 -0.4488381032013251 2203 74-75 -0.45855756187939534 2203 76-77 -0.458390702472002 2203 78-79 -0.41993937102532186 2203 80-81 -0.4403830140288534 2203 82-83 -0.47626864277058445 2203 84-85 -0.5698004173516864 2203 86-87 -0.5142570328364329 2203 88-89 -0.5879695604405555 2203 90-91 -0.5999796640562209 2203 92-93 -0.674275608481878 2203 94-95 -0.6411974144179524 2203 96-97 -0.7477608253528487 2203 98-99 -0.6497643961406396 2203 100-101 -0.7753918718109531 1103 1 0.14296705555027955 1103 2 0.08632115841706423 1103 3 0.05990360300365438 1103 4 0.08120401197492555 1103 5 0.03964389645193478 1103 6 0.020099795581138835 1103 7 0.011693340714181488 1103 8 0.036916610319430276 1103 9 0.0058588177412701725 1103 10-11 0.04578128490159372 1103 12-13 0.012182817263898471 1103 14-15 0.007485060978652314 1103 16-17 0.027800865296569555 1103 18-19 0.010248258862112891 1103 20-21 0.006471838058168089 1103 22-23 0.010945165272829627 1103 24-25 -0.0028588163561877877 1103 26-27 -0.013704065896931183 1103 28-29 -0.06106656067579763 1103 30-31 -0.006977646097304557 1103 32-33 -0.04445950199448845 1103 34-35 -0.03412279990408251 1103 36-37 -0.04879890987786695 1103 38-39 0.003689142622796737 1103 40-41 -0.012476241904714414 1103 42-43 -0.05956048053995033 1103 44-45 -0.05488541216693221 1103 46-47 -0.09484523482529994 1103 48-49 -0.10481727647224659 1103 50-51 -0.11638005889894032 1103 52-53 -0.06191400377398537 1103 54-55 -0.1101712971101918 1103 56-57 -0.08633693035493195 1103 58-59 -0.07267391750885821 1103 60-61 -0.04186265709513748 1103 62-63 -0.09619043179932873 1103 64-65 -0.18441045368956566 1103 66-67 -0.14379953053270356 1103 68-69 -0.16558406001578874 1103 70-71 -0.16915930754060327 1103 72-73 -0.18984845725189814 1103 74-75 -0.19879314421046246 1103 76-77 -0.1747160602034441 1103 78-79 -0.1876698727368762 1103 80-81 -0.21801727864834675 1103 82-83 -0.25765098296040634 1103 84-85 -0.17374296671900424 1103 86-87 -0.2056926843384801 1103 88-89 -0.32870513658563993 1103 90-91 -0.35536039022341015 1103 92-93 -0.29843673852901986 1103 94-95 -0.3807865985915768 1103 96-97 -0.4095807619803793 1103 98-99 -0.27778229713990754 1103 100-101 -0.3139731806104322 2204 1 0.08210019567867732 2204 2 0.056397324819137395 2204 3 0.022481727446923117 2204 4 0.0672293219074902 2204 5 0.06748330797189084 2204 6 0.04983871765463732 2204 7 0.07228713138184162 2204 8 0.09089836134251783 2204 9 0.1167830126716396 2204 10-11 0.053297165383931144 2204 12-13 0.11229452158851672 2204 14-15 0.08082754548228621 2204 16-17 0.07501301892645529 2204 18-19 0.0752975985859905 2204 20-21 0.05748348263647074 2204 22-23 0.04005744001828049 2204 24-25 0.04526855470772517 2204 26-27 0.043048514487075806 2204 28-29 0.006702869407547496 2204 30-31 0.03776206088154055 2204 32-33 0.03321426561453933 2204 34-35 0.037021627857086514 2204 36-37 -0.010196342378584689 2204 38-39 0.030997359263501778 2204 40-41 0.09337363324158332 2204 42-43 0.030675936612496457 2204 44-45 0.003921021002447844 2204 46-47 0.01579123700072671 2204 48-49 0.012109855808255077 2204 50-51 0.01931229778519139 2204 52-53 0.08051062173183965 2204 54-55 0.036094685424259865 2204 56-57 0.061777563709839 2204 58-59 0.033710991055954764 2204 60-61 0.035965016372543346 2204 62-63 0.06442952375250144 2204 64-65 0.04497694314460432 2204 66-67 0.11749235784242273 2204 68-69 0.05086587747187821 2204 70-71 0.1062412078606556 2204 72-73 0.05929209612304476 2204 74-75 0.015498297174367792 2204 76-77 0.014109357026974578 2204 78-79 0.036233099772324806 2204 80-81 0.03590438262985174 2204 82-83 1.9169082877112942E-4 2204 84-85 0.023022851296325086 2204 86-87 0.06719140855089734 2204 88-89 -0.04898350982186628 2204 90-91 0.015706795881285984 2204 92-93 0.009549020866174374 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-0.021336032602391697 1215 6 -0.03059187667195573 1215 7 -0.02520588824127401 1215 8 -0.05577775450947797 1215 9 -0.019380948071010096 1215 10-11 -0.002611111712120362 1215 12-13 -0.06884915878789144 1215 14-15 -0.0322737837014202 1215 16-17 0.022468522132562896 1215 18-19 0.03842464124667799 1215 20-21 0.03917360330115116 1215 22-23 -0.011615822691766198 1215 24-25 0.055128403604662424 1215 26-27 0.01674143512106241 1215 28-29 0.04965817068958245 1215 30-31 0.01170024441633899 1215 32-33 0.05030082299509786 1215 34-35 0.027943191871827366 1215 36-37 0.062351539521870336 1215 38-39 0.07005112917395451 1215 40-41 0.05311722502661098 1215 42-43 0.06344882238171579 1215 44-45 0.1051502607159236 1215 46-47 0.07662800335318565 1215 48-49 0.15588518729570922 1215 50-51 0.15364789833661519 1215 52-53 0.12563228136354354 1215 54-55 0.1436118064610099 1215 56-57 0.11948147365212947 1215 58-59 0.15160655100357445 1215 60-61 0.1875392669206235 1215 62-63 0.15255668460547156 1215 64-65 0.2343671099563096 1215 66-67 0.3033458101648421 1215 68-69 0.296327270801541 1215 70-71 0.27366390986649236 1215 72-73 0.21464664851173154 1215 74-75 0.23676206933695454 1215 76-77 0.2637782666614541 1215 78-79 0.3354558361051687 1215 80-81 0.3470126376591054 1215 82-83 0.3560737884325462 1215 84-85 0.3563868262283947 1215 86-87 0.4041543445858835 1215 88-89 0.396146032738784 1215 90-91 0.3665767956006505 1215 92-93 0.3862204723539051 1215 94-95 0.4146736556785484 1215 96-97 0.4815720284166858 1215 98-99 0.4980322301417601 1215 100-101 0.6240101835180383 1212 1 -0.14948787641101546 1212 2 -0.11118064079796852 1212 3 -0.0423320236899869 1212 4 -0.044794431542520385 1212 5 -0.06418428956057198 1212 6 -0.07806221976520078 1212 7 -0.18563806247442471 1212 8 -0.19362390967032894 1212 9 -0.2301966907724875 1212 10-11 -0.23311759300137425 1212 12-13 -0.17873937411169294 1212 14-15 -0.16007657766079575 1212 16-17 -0.05267863896561664 1212 18-19 -0.03501003648651135 1212 20-21 -0.02802748977074998 1212 22-23 0.02712470019906732 1212 24-25 -0.007121787719704287 1212 26-27 0.02354527735297296 1212 28-29 0.074598340826455 1212 30-31 0.06438023904559742 1212 32-33 -0.010597619914868517 1212 34-35 0.011740414932603471 1212 36-37 -0.054441038699074795 1212 38-39 -0.0716224605091611 1212 40-41 -0.14515916696399955 1212 42-43 -0.19736833288119726 1212 44-45 -0.006154942430043775 1212 46-47 -0.05020746637231355 1212 48-49 -0.1898926904681275 1212 50-51 -0.04927966243540283 1212 52-53 -0.13643799058718997 1212 54-55 -0.1707437589080527 1212 56-57 -0.10804933725137289 1212 58-59 -0.12843935774455417 1212 60-61 -0.14247910671298314 1212 62-63 -0.15376815854332904 1212 64-65 0.05803482484922995 1212 66-67 0.04438271216979928 1212 68-69 -0.08648923587855961 1212 70-71 0.02393074515203608 1212 72-73 -0.01968400101657153 1212 74-75 0.09300547097247147 1212 76-77 -0.03896878549480931 1212 78-79 0.16403378115675338 1212 80-81 0.21689191405775432 1212 82-83 0.20093008998019002 1212 84-85 0.30810057638682764 1212 86-87 0.27156798052928366 1212 88-89 0.26509666047330427 1212 90-91 0.31305940693846424 1212 92-93 0.34086796955533316 1212 94-95 0.2554910950734026 1212 96-97 0.3094023887265891 1212 98-99 0.35657860515856754 1212 100-101 0.39467421781495204 1213 1 -0.29420940304270005 1213 2 -0.20054162222835714 1213 3 -0.1452143045877463 1213 4 -0.15981985059785586 1213 5 -0.11003014543084078 1213 6 -0.042388953515704486 1213 7 -0.10755317328226965 1213 8 -0.10335647022350258 1213 9 -0.07818736587580588 1213 10-11 -0.1177096724174973 1213 12-13 -0.14869003996536634 1213 14-15 -0.21769047012683274 1213 16-17 -0.35173065406016235 1213 18-19 -0.31363014493180685 1213 20-21 -0.16973629999032624 1213 22-23 -0.2371928711377791 1213 24-25 -0.30842792769281147 1213 26-27 -0.1591393767081115 1213 28-29 -0.14305752849046627 1213 30-31 -0.1616906299641485 1213 32-33 -0.13592762655317614 1213 34-35 -0.059553501918585994 1213 36-37 -0.022234180683717852 1213 38-39 0.00627981217592577 1213 40-41 0.01596726204852672 1213 42-43 0.026996821779505353 1213 44-45 0.004794534670857331 1213 46-47 0.06532551863094227 1213 48-49 0.06268866290238861 1213 50-51 0.09124282243458026 1213 52-53 0.0904825518568586 1213 54-55 0.09220620197407925 1213 56-57 0.1377830594736551 1213 58-59 0.11086587989326091 1213 60-61 0.11344065402340675 1213 62-63 0.18023052606908863 1213 64-65 0.1574935218689575 1213 66-67 0.21320099394749548 1213 68-69 0.19662209279755416 1213 70-71 0.18028915445680838 1213 72-73 0.09781608488557225 1213 74-75 0.13971749117760623 1213 76-77 0.20175798293279712 1213 78-79 0.2261024550283537 1213 80-81 0.15954498934392092 1213 82-83 0.30058731968088637 1213 84-85 0.21982635396690142 1213 86-87 0.23231844589000872 1213 88-89 0.20566618339884002 1213 90-91 0.32003589035832647 1213 92-93 0.33045060630635703 1213 94-95 0.4134191160903171 1213 96-97 0.3639803172316576 1213 98-99 0.38283508632977714 1213 100-101 0.3450491392331685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 14.0 14 36.0 15 99.0 16 209.0 17 377.0 18 846.0 19 1811.0 20 3698.0 21 7326.0 22 12736.0 23 20870.0 24 31592.0 25 45769.0 26 63069.0 27 83631.0 28 107114.0 29 134613.0 30 165476.0 31 199854.0 32 242625.0 33 294533.0 34 367321.0 35 474614.0 36 662327.0 37 1061396.0 38 2322405.0 39 7486262.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.665949160466212 33.65055933474575 35.03756056408239 16.645930940705647 2 15.199176171809453 35.74547647925847 33.64043185861551 15.414915490316567 3 15.183309702542083 34.118444105099535 34.45968322098284 16.23856297137555 4 15.792760051521965 33.62542069346686 34.473402688188926 16.108416566822253 5 16.09767013426442 33.91259408657608 34.23162970954974 15.758106069609761 6 15.97795835619609 34.279589834338886 33.322482965417876 16.419968844047148 7 15.965341087201065 34.02511982236046 34.12064125021763 15.88889784022085 8 15.687246326725052 34.29824018827866 33.98349008598089 16.031023399015403 9 15.628312942166744 33.763432688846585 34.36968569632733 16.238568672659344 10-11 15.75598866576893 34.03613624586027 34.100897686676525 16.106977401694277 12-13 15.520611360342459 34.0195181900049 34.3473895269271 16.112480922725535 14-15 15.571953091923932 33.86515427359946 34.28313288679383 16.279759747682785 16-17 15.671949700894586 33.77031262474509 34.357624742551515 16.20011293180881 18-19 15.694809436818048 33.6190250433211 34.290263753856514 16.395901766004332 20-21 15.799521137968433 33.59164434411209 34.1172301937891 16.491604324130382 22-23 15.773446936343655 33.599973953266854 34.16072560165452 16.465853508734973 24-25 15.87911119142362 33.482211599104275 34.092922241413675 16.545754968058436 26-27 15.95862307811621 33.57221498572815 33.959312831257535 16.509849104898095 28-29 15.96305251141511 33.521209437084146 33.949188864050726 16.566549187450022 30-31 16.018184197358025 33.38617315146718 33.95599837869261 16.63964427248218 32-33 16.10181699413755 33.56753712847639 33.83136492815421 16.499280949231856 34-35 16.075968662154555 33.49949426548988 33.87067322879069 16.553863843564873 36-37 16.073743255056325 33.39190611472789 33.81194119675643 16.722409433459358 38-39 16.167564465625116 33.434990974388235 33.830821940210285 16.566622619776368 40-41 16.197304112871393 33.52896074343492 33.68347194958578 16.590263194107898 42-43 16.0938897412668 33.33396427671117 33.81854692144595 16.75359906057608 44-45 16.152443042383027 33.41845204432129 33.71568701823377 16.7134178950619 46-47 16.229800167209042 33.50745986141724 33.67258386705431 16.590156104319412 48-49 16.14076877322088 33.27963590766581 33.77800516747066 16.80159015164265 50-51 16.226867505735086 33.358494241486916 33.643033942821766 16.77160430995623 52-53 16.188523490409892 33.42344963671843 33.74388021651121 16.64414665636048 54-55 16.18704943358764 33.289262499004145 33.753667074775166 16.77002099263305 56-57 16.28833026045186 33.357615412550885 33.628236882809084 16.725817444188174 58-59 16.229188151152147 33.350644208217844 33.770583406217405 16.649584234412597 60-61 16.3051294766925 33.313285904794036 33.56731789808241 16.81426672043105 62-63 16.220535392336604 33.33879011984445 33.64730703959905 16.7933674482199 64-65 16.214254634585206 33.32627779328957 33.68469995264521 16.774767619480006 66-67 16.306421363583922 33.32891837288028 33.62401490968281 16.740645353852976 68-69 16.271886906644724 33.33784381745331 33.65406718764934 16.736202088252625 70-71 16.31463126011756 33.318153324351265 33.57662198365758 16.79059343187359 72-73 16.298735048802364 33.27567462268876 33.618479294629 16.807111033879874 74-75 16.319214351561445 33.324339427469056 33.5678457120046 16.788600508964905 76-77 16.322765917558918 33.36180308129742 33.58868215266693 16.72674884847673 78-79 16.320930520153674 33.244680590645146 33.64728802283144 16.787100866369737 80-81 16.43437945711543 33.292674776402414 33.53087473810098 16.742071028381176 82-83 16.30667977437583 33.45020186360521 33.55722486305053 16.685893498968433 84-85 16.302852826190986 33.1926628465183 33.61732858111895 16.88715574617176 86-87 16.33938213325539 33.45268236149055 33.498253243122306 16.709682262131746 88-89 16.335398994969264 33.395779268304935 33.58608309196359 16.682738644762217 90-91 16.33968459871566 33.22037422158033 33.54507440935683 16.894866770347182 92-93 16.32198695065186 33.30375516865464 33.60821409239092 16.766043788302582 94-95 16.339927156891605 33.316268775274565 33.568259283812175 16.775544784021655 96-97 16.32559845239532 33.297478934800566 33.56949978418468 16.80742282861943 98-99 16.361188643190246 33.31945316034461 33.539396612055725 16.77996158440942 100-101 16.414342961718805 33.361147393428936 33.482442027100404 16.742067617751857 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1183.0 1 1080.5 2 1022.5 3 1250.0 4 1646.0 5 2153.0 6 2857.0 7 3755.0 8 5039.5 9 7044.0 10 9980.5 11 14659.5 12 21838.5 13 32596.5 14 48641.0 15 71198.0 16 101667.0 17 140370.0 18 185423.0 19 236805.5 20 292441.0 21 347714.0 22 401128.5 23 449507.0 24 488582.5 25 515948.0 26 530599.5 27 538822.5 28 548393.5 29 555254.0 30 557332.0 31 559230.0 32 554561.5 33 534533.5 34 501823.0 35 461945.5 36 427846.0 37 402744.0 38 381542.5 39 365260.0 40 347641.5 41 324566.0 42 300461.0 43 275317.5 44 248007.0 45 223213.5 46 202277.5 47 193941.0 48 184160.0 49 161979.0 50 138594.0 51 117812.0 52 101880.0 53 89711.5 54 81393.5 55 74462.5 56 67837.5 57 61572.0 58 55492.0 59 49221.0 60 41491.5 61 33646.0 62 26530.5 63 20727.5 64 16423.5 65 12479.5 66 9370.0 67 7126.5 68 5354.0 69 3730.5 70 2591.0 71 1842.5 72 1322.5 73 975.0 74 717.5 75 528.0 76 377.0 77 276.0 78 219.5 79 169.0 80 116.0 81 76.5 82 48.5 83 34.0 84 27.5 85 16.5 86 9.5 87 7.5 88 5.0 89 3.5 90 2.0 91 1.0 92 1.0 93 2.5 94 2.0 95 1.0 96 0.5 97 0.5 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09625380956320828 2 0.002675731183428044 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 7.97643442214322E-5 10-11 6.88873881912369E-5 12-13 0.0 14-15 0.0014828916721166259 16-17 0.0 18-19 0.0 20-21 8.70156482415624E-5 22-23 1.885339045233852E-4 24-25 3.589395489964449E-4 26-27 0.0013197373316636966 28-29 0.001743938616841313 30-31 0.0037525498304173785 32-33 0.004115115031423889 34-35 0.005648765831681426 36-37 0.006827102734952584 38-39 0.011094495150799208 40-41 0.012004533805325547 42-43 0.012668028123167458 44-45 0.014770906289005218 46-47 0.013741221118146728 48-49 0.016333562305343274 50-51 0.014774531941015284 52-53 0.015554047123179279 54-55 0.01605801275257833 56-57 0.014433720652069163 58-59 0.01162384034426871 60-61 0.00597870016459735 62-63 0.0033029689811693063 64-65 3.009291168354033E-4 66-67 0.002828008567850778 68-69 0.003835939826648876 70-71 0.0040353506872024564 72-73 0.016710630114390047 74-75 0.012011785109345677 76-77 0.03367868152149471 78-79 0.025346933202365113 80-81 0.0190528013128921 82-83 0.03142715162324429 84-85 0.011185136451050834 86-87 0.01604713579654813 88-89 0.007936552250032505 90-91 0.005982325816607415 92-93 0.012581012474925898 94-95 0.013124860276435661 96-97 0.00765012574123736 98-99 0.00848040005154227 100-101 0.0017076820967406622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3790623E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.3587853849602 #Duplication Level Percentage of deduplicated Percentage of total 1 46.63458154476603 19.28749649627404 2 24.153841741528232 19.97947113620319 3 12.777139010210888 15.853408504713448 4 7.072240390129471 11.699970899448479 5 3.557533136431591 7.356762474477926 6 1.9736253593250743 4.8976048599984425 7 1.2086933032465443 3.499306084764871 8 0.6271918954819277 2.075191600033875 9 0.3997725667908267 1.4880697013426822 >10 1.5024845710408876 11.022887286562845 >50 0.08233273466262109 2.2257922356319915 >100 0.01056374638584209 0.6140387205481721 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.80104321610416E-5 0.0 1.45026080402604E-5 2 5.80104321610416E-5 0.0 1.45026080402604E-5 3 5.80104321610416E-5 0.0 1.45026080402604E-5 4 5.80104321610416E-5 7.2513040201302E-6 4.35078241207812E-5 5 5.80104321610416E-5 7.2513040201302E-6 5.80104321610416E-5 6 5.80104321610416E-5 1.45026080402604E-5 5.80104321610416E-5 7 5.80104321610416E-5 3.6256520100651E-5 5.80104321610416E-5 8 5.80104321610416E-5 3.6256520100651E-5 5.80104321610416E-5 9 5.80104321610416E-5 3.6256520100651E-5 5.80104321610416E-5 10-11 5.80104321610416E-5 3.6256520100651E-5 6.52617361811718E-5 12-13 6.52617361811718E-5 3.6256520100651E-5 7.2513040201302E-5 14-15 9.42669522616926E-5 5.80104321610416E-5 1.051439082918879E-4 16-17 1.377747763824738E-4 6.52617361811718E-5 1.305234723623436E-4 18-19 1.994108605535805E-4 6.52617361811718E-5 1.595286884428644E-4 20-21 2.791752047750127E-4 6.52617361811718E-5 1.740312964831248E-4 22-23 3.553138969863798E-4 6.52617361811718E-5 1.740312964831248E-4 24-25 4.060730251272912E-4 6.52617361811718E-5 2.066621645737107E-4 26-27 4.4595519723800733E-4 6.52617361811718E-5 2.320417286441664E-4 28-29 5.039656293990489E-4 6.52617361811718E-5 2.90052160805208E-4 30-31 5.873556256305462E-4 6.52617361811718E-5 2.936778128152731E-4 32-33 7.940177902042569E-4 6.52617361811718E-5 2.973034648253382E-4 34-35 9.82551694727642E-4 7.61386922113671E-5 3.118060728655986E-4 36-37 0.0011094495150799208 7.97643442214322E-5 3.118060728655986E-4 38-39 0.0013197373316636966 7.97643442214322E-5 3.118060728655986E-4 40-41 0.001486517324126691 7.97643442214322E-5 3.335599849259892E-4 42-43 0.0017113077487507272 7.97643442214322E-5 3.335599849259892E-4 44-45 0.001852708177143266 8.70156482415624E-5 3.408112889461194E-4 46-47 0.0020484933856867813 8.70156482415624E-5 3.553138969863798E-4 48-49 0.0022406529422202316 8.70156482415624E-5 3.6256520100651E-4 50-51 0.002494448582924789 9.42669522616926E-5 3.661908530165751E-4 52-53 0.0028135059598105175 9.42669522616926E-5 3.770678090467704E-4 54-55 0.003114435076645921 9.42669522616926E-5 3.951960690970959E-4 56-57 0.0034624976696121705 9.42669522616926E-5 4.133243291474214E-4 58-59 0.003843191130669006 9.42669522616926E-5 4.35078241207812E-4 60-61 0.0041332432914742145 9.42669522616926E-5 4.35078241207812E-4 62-63 0.004564695880671961 9.42669522616926E-5 4.71334761308463E-4 64-65 0.005050533250020684 1.015182562818228E-4 4.71334761308463E-4 66-67 0.005467483231178171 1.015182562818228E-4 4.967143253789188E-4 68-69 0.0059533206005268945 1.015182562818228E-4 5.365964974896349E-4 70-71 0.006392024493744771 1.015182562818228E-4 5.365964974896349E-4 72-73 0.006917744035204211 1.015182562818228E-4 5.510991055298953E-4 74-75 0.007341945320381828 1.2327216834221341E-4 5.728530175902858E-4 76-77 0.007838659645760747 1.2327216834221341E-4 6.381147537714576E-4 78-79 0.008578292655814026 1.2327216834221341E-4 6.52617361811718E-4 80-81 0.009361433489988087 1.2327216834221341E-4 6.52617361811718E-4 82-83 0.01019533345230306 1.2327216834221341E-4 6.634943178419133E-4 84-85 0.010992976894517382 1.2327216834221341E-4 6.88873881912369E-4 86-87 0.012178565101808671 1.2327216834221341E-4 6.961251859324992E-4 88-89 0.013860867634478878 1.2327216834221341E-4 6.961251859324992E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 1125 0.0 15.197139 6 TGCCGTC 1135 0.0 14.2263975 5 TATGCCG 1315 0.0 11.556717 3 ATGCCGT 1545 0.0 10.451069 4 CGTATGC 1815 0.0 8.904436 1 GTATGCC 2740 1.6370905E-11 5.7198505 2 CCGTCTT 3105 0.0 5.65916 7 AGATCGG 665 8.1081374E-4 5.357679 94-95 >>END_MODULE