FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n01 3-58925.34100000001ae7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n01 3-58925.34100000001ae7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13790623
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT11250.015.1971396
TGCCGTC11350.014.22639755
TATGCCG13150.011.5567173
ATGCCGT15450.010.4510694
CGTATGC18150.08.9044361
GTATGCC27401.6370905E-115.71985052
CCGTCTT31050.05.659167
AGATCGG6658.1081374E-45.35767994-95