##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n01 2-58885.34100000001acd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12133707 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.78067593028248 38.0 38.0 38.0 32.0 38.0 2 36.77443818282409 38.0 38.0 38.0 32.0 38.0 3 36.759886240866045 38.0 38.0 38.0 32.0 38.0 4 36.75905434340882 38.0 38.0 38.0 32.0 38.0 5 36.74654769560531 38.0 38.0 38.0 32.0 38.0 6 38.69341652967226 40.0 38.0 40.0 38.0 40.0 7 38.64168032077913 40.0 38.0 40.0 38.0 40.0 8 38.632960809091564 40.0 38.0 40.0 38.0 40.0 9 38.63187746333416 40.0 38.0 40.0 38.0 40.0 10-11 38.646896286518206 40.0 38.0 40.0 38.0 40.0 12-13 38.63990258706593 40.0 38.0 40.0 38.0 40.0 14-15 38.625696046558566 40.0 38.0 40.0 38.0 40.0 16-17 38.60834368260252 40.0 38.0 40.0 38.0 40.0 18-19 38.579013116107056 40.0 38.0 40.0 38.0 40.0 20-21 38.55372661462816 40.0 38.0 40.0 38.0 40.0 22-23 38.53434877733573 40.0 38.0 40.0 38.0 40.0 24-25 38.49015300929881 40.0 38.0 40.0 38.0 40.0 26-27 38.413228949734815 40.0 38.0 40.0 38.0 40.0 28-29 38.3714188087779 40.0 38.0 40.0 38.0 40.0 30-31 38.32061673320445 40.0 38.0 40.0 38.0 40.0 32-33 38.284441803316994 40.0 38.0 40.0 38.0 40.0 34-35 38.25157484847788 40.0 38.0 40.0 38.0 40.0 36-37 38.21109381494048 40.0 38.0 40.0 38.0 40.0 38-39 38.159225989221596 40.0 38.0 40.0 38.0 40.0 40-41 38.11316298473335 40.0 38.0 40.0 38.0 40.0 42-43 38.07422776897448 40.0 38.0 40.0 38.0 40.0 44-45 38.04690433846804 40.0 38.0 40.0 38.0 40.0 46-47 38.00837069001254 40.0 38.0 40.0 38.0 40.0 48-49 37.9614616950945 40.0 38.0 40.0 38.0 40.0 50-51 37.90607388162579 40.0 38.0 40.0 35.0 40.0 52-53 37.85960766153328 40.0 38.0 40.0 32.0 40.0 54-55 37.80670779342208 40.0 38.0 40.0 32.0 40.0 56-57 37.75205318539503 40.0 38.0 40.0 32.0 40.0 58-59 37.72275513163454 40.0 38.0 40.0 32.0 40.0 60-61 37.6599108994473 40.0 38.0 40.0 32.0 40.0 62-63 37.620925039643694 40.0 38.0 40.0 32.0 40.0 64-65 37.57444719902994 40.0 38.0 40.0 32.0 40.0 66-67 37.482182155873716 40.0 38.0 40.0 32.0 40.0 68-69 37.42542184346466 40.0 38.0 40.0 32.0 40.0 70-71 37.39897835838627 40.0 38.0 40.0 32.0 40.0 72-73 37.34560971350305 40.0 38.0 40.0 32.0 40.0 74-75 37.269650940145496 39.0 38.0 40.0 32.0 40.0 76-77 37.19614904167375 38.0 38.0 40.0 32.0 40.0 78-79 37.10876564762937 38.0 38.0 40.0 32.0 40.0 80-81 37.06146303845972 38.0 38.0 40.0 32.0 40.0 82-83 36.979354742948715 38.0 38.0 40.0 32.0 40.0 84-85 36.904602814292446 38.0 38.0 40.0 32.0 40.0 86-87 36.84211964241431 38.0 38.0 40.0 32.0 40.0 88-89 36.79308615248415 38.0 38.0 40.0 32.0 40.0 90-91 36.7238782014433 38.0 38.0 40.0 32.0 40.0 92-93 36.6515667058715 38.0 38.0 40.0 32.0 40.0 94-95 36.559377525763566 38.0 38.0 40.0 32.0 40.0 96-97 36.472262969593714 38.0 38.0 40.0 32.0 40.0 98-99 36.36567662298093 38.0 38.0 40.0 32.0 40.0 100-101 35.48339814864492 38.0 35.0 39.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.09814936005835762 2201 2 0.12648636844855332 2201 3 0.11508053754548087 2201 4 0.03569253577408915 2201 5 0.06594633422452745 2201 6 0.11963877949406765 2201 7 0.16460826601640832 2201 8 0.1134888214333003 2201 9 0.1149660946592519 2201 10-11 0.06132174866939977 2201 12-13 0.010757540360103235 2201 14-15 0.036211620740644435 2201 16-17 0.07357015717097681 2201 18-19 0.01872273515228784 2201 20-21 0.10054815557973029 2201 22-23 0.1174084667579578 2201 24-25 0.03574299799469571 2201 26-27 0.043422408958726066 2201 28-29 0.08347149719571689 2201 30-31 0.07761581896993874 2201 32-33 0.020185003504984422 2201 34-35 0.03558736111856575 2201 36-37 0.07886833354115907 2201 38-39 0.06309463984209174 2201 40-41 0.03079551104089262 2201 42-43 -0.05172621956305079 2201 44-45 -0.03468052201034766 2201 46-47 -0.03667989554394069 2201 48-49 -0.026150734980241452 2201 50-51 -0.05824653711258776 2201 52-53 -0.07738507769064285 2201 54-55 -0.10189089202277302 2201 56-57 -0.14006564232272467 2201 58-59 -0.03461464228469424 2201 60-61 -0.0571694511202665 2201 62-63 -0.049663193959311513 2201 64-65 -0.16189411115139762 2201 66-67 -0.08054491187898805 2201 68-69 -0.06960139566420054 2201 70-71 -0.05041823989272842 2201 72-73 -0.10973923135497898 2201 74-75 -0.12322354970116578 2201 76-77 -0.09554446824472507 2201 78-79 -0.162451535877139 2201 80-81 -0.1591143355375948 2201 82-83 -0.195618113465315 2201 84-85 -0.22318116977631064 2201 86-87 -0.30053940058077444 2201 88-89 -0.26303399661154003 2201 90-91 -0.20270121158373655 2201 92-93 -0.27197094540795774 2201 94-95 -0.27257314825821055 2201 96-97 -0.2327083734576334 2201 98-99 -0.280157003192798 2201 100-101 -0.4115679558334975 1101 1 0.16156591546695864 1101 2 0.09865350922066796 1101 3 0.13270663309590702 1101 4 0.052876340910657404 1101 5 0.003500680966411096 1101 6 0.07325520327447066 1101 7 0.05245025280234472 1101 8 0.06890612735521273 1101 9 0.06266619868920742 1101 10-11 0.10983926031592262 1101 12-13 0.038583426753866945 1101 14-15 0.065576139802765 1101 16-17 0.053581996334209236 1101 18-19 0.05453800033602363 1101 20-21 0.018096460301748607 1101 22-23 0.021717380422543897 1101 24-25 0.009520934359144917 1101 26-27 -0.023553494628394844 1101 28-29 0.026589184642752173 1101 30-31 -0.007196854304986289 1101 32-33 -0.027140921700286924 1101 34-35 -0.018790392146350143 1101 36-37 0.03773877601405218 1101 38-39 -0.01816798630593297 1101 40-41 0.0229803585570707 1101 42-43 0.05271459175905591 1101 44-45 -0.02679731939283414 1101 46-47 -0.09624706832963881 1101 48-49 -0.09054581538340045 1101 50-51 -0.05461848879041753 1101 52-53 -0.09702427772550237 1101 54-55 -0.08129561006452235 1101 56-57 -0.038481270226199626 1101 58-59 -0.06087717199487486 1101 60-61 -0.08018749747679976 1101 62-63 -0.0570328296162117 1101 64-65 -0.12197300486942453 1101 66-67 -0.07810902909785966 1101 68-69 -0.09191542214168891 1101 70-71 -0.15991413140431376 1101 72-73 -0.15564808757287096 1101 74-75 -0.18784668905355772 1101 76-77 -0.19864419083465634 1101 78-79 -0.22067358986890895 1101 80-81 -0.186795127889809 1101 82-83 -0.2968539384026414 1101 84-85 -0.2884029210288901 1101 86-87 -0.2461223394407881 1101 88-89 -0.2207329320417699 1101 90-91 -0.29745846997314374 1101 92-93 -0.29384342602843816 1101 94-95 -0.34339973448815186 1101 96-97 -0.3388843568234279 1101 98-99 -0.3500416777164972 1101 100-101 -0.3687479114928962 2202 1 0.10697820244878642 2202 2 0.11582600876705129 2202 3 0.0779012548391691 2202 4 0.04795506584761711 2202 5 0.06693988890326352 2202 6 0.07612952315345467 2202 7 0.06861615646631236 2202 8 0.04678251281060852 2202 9 0.0023323095851637277 2202 10-11 0.03629793734683773 2202 12-13 0.015783026540105993 2202 14-15 0.021131380151331314 2202 16-17 0.026729342117960186 2202 18-19 0.041798420868225605 2202 20-21 -0.024537877760749893 2202 22-23 -0.0020153185263396267 2202 24-25 0.028329645523612612 2202 26-27 0.0422942593095641 2202 28-29 -0.0433120800386817 2202 30-31 2.012369138952863E-4 2202 32-33 -0.02052088916632755 2202 34-35 -0.061365531195164635 2202 36-37 -0.031928004939409504 2202 38-39 -0.008891105639420971 2202 40-41 -0.003162221680206301 2202 42-43 -0.07549852134912527 2202 44-45 -0.10040069586349176 2202 46-47 -0.12153770845540635 2202 48-49 -0.1042160594398851 2202 50-51 -0.13631800521029191 2202 52-53 -0.14681279389005653 2202 54-55 -0.15454568331183083 2202 56-57 -0.22140502899255665 2202 58-59 -0.18068130477532662 2202 60-61 -0.13843124050447386 2202 62-63 -0.1531447646078803 2202 64-65 -0.21928663137008897 2202 66-67 -0.07346329832589049 2202 68-69 -0.12175573473239609 2202 70-71 -0.17797668518075938 2202 72-73 -0.2058809900294989 2202 74-75 -0.2219372402449835 2202 76-77 -0.23168797449491052 2202 78-79 -0.18802273891962784 2202 80-81 -0.269357805049097 2202 82-83 -0.291562570363304 2202 84-85 -0.30383072117538035 2202 86-87 -0.31509298934339824 2202 88-89 -0.3080930397205748 2202 90-91 -0.3629084653913921 2202 92-93 -0.3678632895246139 2202 94-95 -0.3683502480132077 2202 96-97 -0.45998311672506986 2202 98-99 -0.4184436025164615 2202 100-101 -0.45027364324143804 1102 1 0.07255275882040735 1102 2 0.03667295852901731 1102 3 -0.03422825064345858 1102 4 -0.06133762375192475 1102 5 -0.02052890140381436 1102 6 -0.01295243880001351 1102 7 0.044487603845269064 1102 8 -0.018858756294278578 1102 9 0.009359734872596448 1102 10-11 -0.015299477869810119 1102 12-13 -0.02169662799945371 1102 14-15 -0.010175189504231241 1102 16-17 -0.03949193017945163 1102 18-19 -0.09076912076977095 1102 20-21 -0.04384564236214317 1102 22-23 -0.08459987752750919 1102 24-25 -0.0656936657757683 1102 26-27 -0.09167965901925612 1102 28-29 -0.10506853106584657 1102 30-31 -0.0846581620102782 1102 32-33 -0.07340904000712811 1102 34-35 -0.13000573593004816 1102 36-37 -0.16204022479806923 1102 38-39 -0.10843395103908193 1102 40-41 -0.23529299131409687 1102 42-43 -0.2487422212223862 1102 44-45 -0.2841090638541033 1102 46-47 -0.2619492700970838 1102 48-49 -0.29741142126122355 1102 50-51 -0.3309129077420394 1102 52-53 -0.240073449988067 1102 54-55 -0.3145633045012488 1102 56-57 -0.31370414850705686 1102 58-59 -0.3143480473698901 1102 60-61 -0.2725900408609263 1102 62-63 -0.29654798625615797 1102 64-65 -0.38630431147539923 1102 66-67 -0.29804224776718513 1102 68-69 -0.3296546543563963 1102 70-71 -0.42681512414336 1102 72-73 -0.46067635733159307 1102 74-75 -0.41646512996375407 1102 76-77 -0.4258592314519589 1102 78-79 -0.41839512002914603 1102 80-81 -0.4423016684194039 1102 82-83 -0.5146782420890048 1102 84-85 -0.48927832796523774 1102 86-87 -0.5012653503692803 1102 88-89 -0.5153022867464188 1102 90-91 -0.4935468724018719 1102 92-93 -0.5245784942215224 1102 94-95 -0.5765830437306718 1102 96-97 -0.6742210574550782 1102 98-99 -0.7921475490832321 1102 100-101 -0.8458223075982474 2203 1 0.0816003690214302 2203 2 0.041897923291898564 2203 3 0.021056549106418743 2203 4 -0.01224494058796921 2203 5 -0.013120657827677462 2203 6 -0.12055231884182405 2203 7 -0.005256295121533583 2203 8 -0.07597791389270014 2203 9 -0.03878422186486574 2203 10-11 -0.0662074981813916 2203 12-13 -0.05708441155935162 2203 14-15 -0.030926776021253488 2203 16-17 -0.05592241818217758 2203 18-19 -0.0832345673978594 2203 20-21 -0.04537362898952324 2203 22-23 -0.003297219996561296 2203 24-25 -0.04610771064390207 2203 26-27 -0.09826909590494637 2203 28-29 -0.0552400535032902 2203 30-31 -0.0741756831285798 2203 32-33 -0.08856285420832677 2203 34-35 -0.1267100818792528 2203 36-37 -0.13888201324314053 2203 38-39 -0.09385438777020028 2203 40-41 -0.09651193358584464 2203 42-43 -0.059697171773976265 2203 44-45 -0.11326049281583295 2203 46-47 -0.15432241260312907 2203 48-49 -0.23284610210159684 2203 50-51 -0.2159073994704741 2203 52-53 -0.2138432591133821 2203 54-55 -0.3589094987976651 2203 56-57 -0.252700658869081 2203 58-59 -0.21538882527706704 2203 60-61 -0.20476778421462427 2203 62-63 -0.28626676381371396 2203 64-65 -0.2524195142998522 2203 66-67 -0.24957141992145182 2203 68-69 -0.32075986314557525 2203 70-71 -0.38629472241250795 2203 72-73 -0.44521210097104813 2203 74-75 -0.4248436960847357 2203 76-77 -0.3830301159191336 2203 78-79 -0.35041318387265363 2203 80-81 -0.4244020617696833 2203 82-83 -0.44300808675718173 2203 84-85 -0.5538232797292864 2203 86-87 -0.48598665110993267 2203 88-89 -0.5847548788145787 2203 90-91 -0.5593403262766472 2203 92-93 -0.6031729142985327 2203 94-95 -0.5712589008951028 2203 96-97 -0.7016132861092288 2203 98-99 -0.6757506438703373 2203 100-101 -0.7328446891771634 1103 1 0.03785575752338133 1103 2 0.061114790251075135 1103 3 0.054393609467894066 1103 4 0.06075518459213214 1103 5 0.07307992908611993 1103 6 0.06518272030406536 1103 7 0.037096952884269285 1103 8 0.06388113411568241 1103 9 -0.03851801352691808 1103 10-11 0.03939783377017392 1103 12-13 0.007460112091543181 1103 14-15 0.009978706945453553 1103 16-17 0.014480943224675968 1103 18-19 0.014215125251141103 1103 20-21 0.013648316312711017 1103 22-23 1.2771345266315848E-4 1103 24-25 0.03271858813705819 1103 26-27 0.006549758254813298 1103 28-29 -0.03236291938147673 1103 30-31 0.004566727055397735 1103 32-33 -0.04060281024784018 1103 34-35 -0.03475430375576849 1103 36-37 -0.029402168327202105 1103 38-39 -0.014795237640200298 1103 40-41 -0.0012866465720335896 1103 42-43 -0.027935881851099964 1103 44-45 -0.021521087737610856 1103 46-47 -0.10662658551857618 1103 48-49 -0.07966360913477644 1103 50-51 -0.1334688636218999 1103 52-53 -0.052159743964260485 1103 54-55 -0.0744529974200816 1103 56-57 -0.002815259326879982 1103 58-59 -0.08994630161283368 1103 60-61 -0.025368407420387484 1103 62-63 -0.07884150713488935 1103 64-65 -0.13490871231564228 1103 66-67 -0.11056797815631114 1103 68-69 -0.1687662416731044 1103 70-71 -0.14859847875727183 1103 72-73 -0.1289707202458601 1103 74-75 -0.15874116500091162 1103 76-77 -0.14317971266634544 1103 78-79 -0.20608857820867854 1103 80-81 -0.21691621520692195 1103 82-83 -0.2598650921069563 1103 84-85 -0.2483374389807551 1103 86-87 -0.2361467453339401 1103 88-89 -0.2776475771521376 1103 90-91 -0.3084328123320148 1103 92-93 -0.2827724835090848 1103 94-95 -0.39038804604105337 1103 96-97 -0.4105703391687854 1103 98-99 -0.301607661457858 1103 100-101 -0.282461968995662 2204 1 0.1176554391743565 2204 2 0.056822993266216315 2204 3 0.05853736970669132 2204 4 0.08663467691423676 2204 5 0.09436481225242233 2204 6 0.09280563570036549 2204 7 0.14817479714631787 2204 8 0.12982675116530373 2204 9 0.14667994298932996 2204 10-11 0.10937865077813314 2204 12-13 0.1709853736519662 2204 14-15 0.12722174209252302 2204 16-17 0.10341371773645847 2204 18-19 0.11321963785930933 2204 20-21 0.10955604353429038 2204 22-23 0.07954677508257646 2204 24-25 0.1269577591708213 2204 26-27 0.07510144812268749 2204 28-29 0.10176921375306591 2204 30-31 0.08407753522070749 2204 32-33 0.08450693218469496 2204 34-35 0.051485363534730766 2204 36-37 0.025523583459737154 2204 38-39 0.05534781841490144 2204 40-41 0.06339569062585326 2204 42-43 0.05587928623938865 2204 44-45 0.0431400778165667 2204 46-47 0.05745109942068183 2204 48-49 0.05721490632604542 2204 50-51 0.06823045323424282 2204 52-53 0.14242867681070237 2204 54-55 0.07603827533181828 2204 56-57 0.0710189945473303 2204 58-59 0.12438643984811648 2204 60-61 0.09821929977957211 2204 62-63 0.10335865172658742 2204 64-65 0.08323639137516636 2204 66-67 0.13453107465564784 2204 68-69 0.17067579736872318 2204 70-71 0.15303905314642208 2204 72-73 0.1491301141146124 2204 74-75 0.06412338640161863 2204 76-77 0.06240180812989138 2204 78-79 0.09995940250254876 2204 80-81 0.07547012395885844 2204 82-83 0.028823088135077057 2204 84-85 0.04192863832885507 2204 86-87 0.05630362775655584 2204 88-89 0.0027267622225508603 2204 90-91 0.027772205957155904 2204 92-93 -0.008339632301584743 2204 94-95 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-0.19378627542937465 1215 2 -0.24804584521181283 1215 3 -0.12998778908711728 1215 4 -0.12441360842151994 1215 5 -0.11655524965624409 1215 6 -0.08594055004523682 1215 7 -0.13041931923520877 1215 8 -0.1497235972013513 1215 9 -0.14123266579895244 1215 10-11 -0.09434433330377345 1215 12-13 -0.15666113670304327 1215 14-15 -0.112446472290749 1215 16-17 -0.062122291036928345 1215 18-19 -0.09606120759836045 1215 20-21 -0.05359065034731714 1215 22-23 -0.09042962782121577 1215 24-25 -0.04279055272881749 1215 26-27 -0.06083579788495541 1215 28-29 -0.040091741741264286 1215 30-31 -0.07998268341278703 1215 32-33 -0.02670742698196449 1215 34-35 -0.046497215941464276 1215 36-37 -0.04419262171944638 1215 38-39 -0.030403464758393284 1215 40-41 -0.02128532940641037 1215 42-43 -0.04444318949784787 1215 44-45 -0.05138432172594065 1215 46-47 -0.02733881062311383 1215 48-49 0.03916400936198983 1215 50-51 0.07440314435177697 1215 52-53 0.03983852863097326 1215 54-55 0.07401869505786607 1215 56-57 0.017156456175243306 1215 58-59 0.056604324832967734 1215 60-61 0.05535614718289139 1215 62-63 0.08735068537861679 1215 64-65 0.18129560285560586 1215 66-67 0.2593615003601428 1215 68-69 0.22440992450935227 1215 70-71 0.14287725134303741 1215 72-73 0.10687558328224611 1215 74-75 0.16575460290853528 1215 76-77 0.17500266950502663 1215 78-79 0.2883070340078575 1215 80-81 0.24625029561621403 1215 82-83 0.2766585228948699 1215 84-85 0.2040808846463804 1215 86-87 0.33803571763722573 1215 88-89 0.3333945152331097 1215 90-91 0.3171606935707203 1215 92-93 0.34084063818259835 1215 94-95 0.3043261645219033 1215 96-97 0.3426315931080808 1215 98-99 0.40274268889152864 1215 100-101 0.4787516481030778 1212 1 -0.19311500243011182 1212 2 -0.16489858016591796 1212 3 -0.10596947847928817 1212 4 -0.15595574793162825 1212 5 -0.08130262605842375 1212 6 -0.13427063898293312 1212 7 -0.23594610300478536 1212 8 -0.2526691173757172 1212 9 -0.31176100484069735 1212 10-11 -0.31718229959012945 1212 12-13 -0.2419579855438272 1212 14-15 -0.19476909097751616 1212 16-17 -0.11727795023234933 1212 18-19 -0.06575346860599751 1212 20-21 -0.07123955891734113 1212 22-23 -0.0687009142106021 1212 24-25 -0.03928155729728644 1212 26-27 -0.04817359620790995 1212 28-29 0.012442607723194499 1212 30-31 0.0059802348331814414 1212 32-33 -0.12736559766224786 1212 34-35 -0.02830851642718102 1212 36-37 -0.19436471615613016 1212 38-39 -0.22340457517476864 1212 40-41 -0.21456018882632577 1212 42-43 -0.2790751563642573 1212 44-45 -0.07451224338637275 1212 46-47 -0.13224097893422737 1212 48-49 -0.28690095813736605 1212 50-51 -0.14840854799860637 1212 52-53 -0.21333027910706193 1212 54-55 -0.3504311061854395 1212 56-57 -0.2246704955477341 1212 58-59 -0.1992259792407154 1212 60-61 -0.2764111634233899 1212 62-63 -0.25328046856590447 1212 64-65 -0.021080351952399212 1212 66-67 -0.0532133727096209 1212 68-69 -0.20984141583908666 1212 70-71 -0.04150598310265252 1212 72-73 -0.11844097628589623 1212 74-75 -0.02287742384135072 1212 76-77 -0.12945005811223353 1212 78-79 0.08966669474276756 1212 80-81 0.11162694917294402 1212 82-83 0.09815302732517495 1212 84-85 0.17602102025272615 1212 86-87 0.14996889734622698 1212 88-89 0.1231833243556153 1212 90-91 0.2693359962931652 1212 92-93 0.2628123924122363 1212 94-95 0.19665074590321296 1212 96-97 0.24157548326830636 1212 98-99 0.3001434777351619 1212 100-101 0.3194795664222241 1213 1 -0.26884306398093827 1213 2 -0.17100664793789377 1213 3 -0.1505673745335514 1213 4 -0.17253091615733496 1213 5 -0.08574932188223272 1213 6 -0.06630636654461597 1213 7 -0.11781203884553548 1213 8 -0.17571108940116176 1213 9 -0.09264602201953664 1213 10-11 -0.13507563911685594 1213 12-13 -0.16677147846245077 1213 14-15 -0.29982349128412267 1213 16-17 -0.4109651741828202 1213 18-19 -0.43224612163229637 1213 20-21 -0.23728427095683458 1213 22-23 -0.3355969628917066 1213 24-25 -0.3939132291735348 1213 26-27 -0.2620587492229234 1213 28-29 -0.23671401138751236 1213 30-31 -0.2627860833373532 1213 32-33 -0.2538950856527009 1213 34-35 -0.1431317210434102 1213 36-37 -0.0929303114477662 1213 38-39 -0.08002156676070626 1213 40-41 -0.0962961723541298 1213 42-43 -0.07098249430632109 1213 44-45 -0.0925371744270862 1213 46-47 -0.043156502046791445 1213 48-49 -0.06756812952167479 1213 50-51 -0.028804034202252637 1213 52-53 -0.0051665237745766035 1213 54-55 -0.004044980000507792 1213 56-57 -0.025911220285223635 1213 58-59 -0.005609602480539877 1213 60-61 -0.022848461137897402 1213 62-63 0.03601956588239119 1213 64-65 -0.012890977033116258 1213 66-67 0.037120681533949096 1213 68-69 0.02009963263682124 1213 70-71 0.03631767247754425 1213 72-73 -0.03241616639793676 1213 74-75 -0.0462401672753856 1213 76-77 0.05024159738413658 1213 78-79 0.08391825694917543 1213 80-81 0.06458782150827602 1213 82-83 0.1821850183481999 1213 84-85 0.045217489034648395 1213 86-87 0.06197886851693113 1213 88-89 0.046187704387094186 1213 90-91 0.1639888431946659 1213 92-93 0.14487470873614683 1213 94-95 0.19660305395833433 1213 96-97 0.12833372570003831 1213 98-99 0.15420911792259773 1213 100-101 0.07535948435043593 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 7.0 14 19.0 15 46.0 16 123.0 17 270.0 18 723.0 19 1869.0 20 4252.0 21 8902.0 22 15746.0 23 25128.0 24 36225.0 25 49779.0 26 66203.0 27 86075.0 28 107830.0 29 132491.0 30 161275.0 31 194697.0 32 233062.0 33 283278.0 34 350300.0 35 451833.0 36 625610.0 37 992655.0 38 2142281.0 39 6163028.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.198045177994906 29.44490654317783 30.003805405754996 21.35324287307227 2 19.912867005356947 30.840359252793487 29.645006521664243 19.60176722018533 3 19.843259772137237 29.200589729091035 30.380435261870094 20.575715236901633 4 20.279565016692754 28.835573497860135 30.132918159306136 20.751943326140974 5 20.828886011504974 29.05412995385499 29.817969067491077 20.299014967148953 6 20.733861465420254 29.29045509340221 28.943331168290122 21.03235227288742 7 20.683645978924663 29.6536911596761 29.454024231836158 20.208638629563083 8 20.03261657793451 29.544474742961903 29.930712848101575 20.492195831002018 9 20.08980772559071 28.922496847622735 30.221680114062487 20.766015312724065 10-11 20.296078726531732 29.211678533136453 29.66539427364212 20.82684846668969 12-13 19.943777280924948 29.355966812120975 30.098110165343535 20.60214574161054 14-15 20.0885396642119 29.10138380827697 30.045118718395987 20.764957809115142 16-17 20.113366838345446 29.16089452300109 29.98382934415674 20.741909294496725 18-19 20.070758260439288 28.9316611980164 30.10919086805047 20.888389673493847 20-21 20.161903783911374 28.885199619439696 29.882027624247463 21.070868972401467 22-23 20.20200542555748 28.838661036499712 29.942682700488987 21.016650837453817 24-25 20.294561405055866 28.806139149854697 29.81903574574776 21.080263699341675 26-27 20.46359483901967 28.803501201936204 29.626286129427527 21.106617829616606 28-29 20.431410901714493 28.893271516119384 29.628944658115724 21.0463729240504 30-31 20.646119065809852 28.661962254260796 29.57393254426458 21.117986135664772 32-33 20.636068144677548 28.852423968396252 29.50630515631269 21.00520273061351 34-35 20.63448184916406 28.686575098594336 29.562377143422303 21.1165659088193 36-37 20.63085488839988 28.688270100257014 29.484822157771294 21.196052853571818 38-39 20.648907107484664 28.659126342905612 29.575271270199348 21.116695279410376 40-41 20.831039182664725 28.690374886748632 29.31557765120294 21.163008279383703 42-43 20.691772935617802 28.647311709370832 29.469904400599102 21.191010954412267 44-45 20.670648081637978 28.48156786647226 29.453035647232845 21.39474840465691 46-47 20.757094819776686 28.713348134595456 29.32865927658615 21.200897769041706 48-49 20.621065155909644 28.41684605470709 29.479639569033484 21.482449220349785 50-51 20.694658354170212 28.579744286463782 29.26864510896242 21.45695225040358 52-53 20.680193314603265 28.590850325513532 29.39030511512669 21.33865124475651 54-55 20.757494965494338 28.39228746126804 29.344130707315173 21.506086865922445 56-57 20.884821863519505 28.600073030063495 29.22398139134635 21.291123715070654 58-59 20.721829883056554 28.512045471779608 29.39196495982645 21.374159685337396 60-61 20.812540743640387 28.449867236362298 29.136814440022125 21.60077757997519 62-63 20.72358922687322 28.529016438657496 29.3035818183489 21.44381251612038 64-65 20.796993852519225 28.42274136443225 29.26329911985818 21.516965663190348 66-67 20.80387137243758 28.532559973128656 29.09411452538151 21.56945412905226 68-69 20.631396534031207 28.487605650959747 29.292065732532414 21.58893208247663 70-71 20.908621198944253 28.419363617602205 29.07951466751415 21.592500515939395 72-73 20.858452306373437 28.493121270071548 29.130555738731907 21.517870684823105 74-75 20.866076992992312 28.344572114300014 29.217909922209795 21.57144097049788 76-77 20.837089982492422 28.54901116310145 29.139529204745678 21.474369649660453 78-79 20.830500629192127 28.46003939612395 29.205128173422086 21.50433180126185 80-81 20.91257405995093 28.419373776520874 29.047100793412923 21.620951370115275 82-83 20.830792944639676 28.624211497081646 29.176381493634373 21.368614064644305 84-85 20.850051954263762 28.451632709051054 29.11272305169038 21.5855922849948 86-87 20.7454314513382 28.589305468336278 29.086577941842744 21.578685138482776 88-89 20.798420995738834 28.47760428122012 29.134094078490875 21.58988064455017 90-91 20.887139845281208 28.307930121041984 29.08746243709295 21.717467596583855 92-93 20.70382170500752 28.421814460380457 29.191104290673298 21.68325954393872 94-95 20.77059181015152 28.327467498046715 29.12574944669591 21.776191245105863 96-97 20.832299287474747 28.43173892047508 29.009397941754898 21.72656385029527 98-99 20.770626239165097 28.43566520628403 29.06243599407055 21.73127256048032 100-101 21.054952034073573 28.340573201041842 28.84565380056173 21.758820964322855 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 240.0 1 223.5 2 238.0 3 278.0 4 323.5 5 449.0 6 582.5 7 751.5 8 1036.5 9 1400.5 10 1760.5 11 2410.5 12 3271.0 13 4502.0 14 6433.5 15 8883.5 16 12178.5 17 16313.0 18 21685.0 19 27592.5 20 34529.0 21 42304.5 22 50239.0 23 59239.0 24 68901.0 25 79226.0 26 89741.5 27 100071.5 28 113897.5 29 131564.0 30 150161.0 31 173626.0 32 208588.0 33 255789.5 34 314410.5 35 388467.5 36 478569.0 37 570698.5 38 641306.0 39 681729.0 40 693217.0 41 663775.0 42 603982.5 43 536288.0 44 470716.5 45 416107.5 46 374697.5 47 351424.0 48 335654.5 49 319985.0 50 304253.5 51 283631.0 52 258107.5 53 236196.5 54 218480.5 55 200901.5 56 186499.0 57 169723.0 58 151012.0 59 132042.5 60 111529.5 61 90333.5 62 71255.5 63 55432.5 64 42121.0 65 31857.0 66 23900.0 67 17204.5 68 11853.5 69 8133.0 70 5659.5 71 4010.0 72 2756.5 73 1939.0 74 1480.5 75 1087.5 76 767.5 77 574.5 78 454.5 79 337.5 80 232.5 81 172.5 82 133.5 83 97.0 84 63.0 85 43.0 86 30.5 87 21.5 88 15.0 89 10.5 90 4.0 91 3.0 92 1.5 93 1.5 94 1.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0945959878543301 2 0.0024806928336080637 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 5.7690531014141016E-5 10-11 7.005278766002837E-5 12-13 0.0 14-15 0.0013351237177558351 16-17 0.0 18-19 0.0 20-21 1.565885841812399E-4 22-23 1.895546019036062E-4 24-25 3.2966017722366295E-4 26-27 0.001314519956679356 28-29 0.0015947311073194698 30-31 0.0034367073475566863 32-33 0.003815816551363899 34-35 0.005414668410898664 36-37 0.006510788500167344 38-39 0.010652144476539609 40-41 0.011562830716119979 42-43 0.01251060372563801 44-45 0.014467961027903509 46-47 0.013462497487371338 48-49 0.01609153740073005 50-51 0.014603945851008271 52-53 0.01546518306400509 54-55 0.015906103551041738 56-57 0.014150663107325732 58-59 0.01110130646800685 60-61 0.005591860756156383 62-63 0.0031853414624236436 64-65 3.0905641614718403E-4 66-67 0.002765024736463473 68-69 0.003922956108961589 70-71 0.0041125107108651955 72-73 0.017364849835256446 74-75 0.012032596468663698 76-77 0.033814892678717234 78-79 0.025317901610777318 80-81 0.01911204877454186 82-83 0.031626773252395164 84-85 0.011550468459474092 86-87 0.01588549978996526 88-89 0.008220900669515096 90-91 0.005991573721040075 92-93 0.012720762088618096 94-95 0.013103992044640602 96-97 0.007779980182478446 98-99 0.00858764761667642 100-101 0.0016318178772571316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2133707E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.876047471438852 #Duplication Level Percentage of deduplicated Percentage of total 1 28.292862571084008 4.774716918525092 2 5.638337906371744 1.903057163358854 3 7.077907464270441 3.58341307096438 4 8.020600407506297 5.414241329060722 5 8.572898729370799 7.233832296234971 6 7.786063838814522 7.883878977569238 7 6.865819288154692 8.110752456605336 8 5.409547669482796 7.303342661536247 9 4.703371931843944 7.143689519786829 >10 17.23923648334139 40.99629546436621 >50 0.31283749765478264 3.6009412352962835 >100 0.07975095889944704 1.8820932257585639 >500 1.0628595925617614E-4 0.016645414375899094 >1k 6.589672458856768E-4 0.15310026656125272 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.6483008861183147E-5 0.0 0.0 2 1.6483008861183147E-5 0.0 0.0 3 1.6483008861183147E-5 0.0 0.0 4 1.6483008861183147E-5 0.0 0.0 5 1.6483008861183147E-5 0.0 0.0 6 2.4724513291774725E-5 0.0 8.241504430591574E-5 7 2.4724513291774725E-5 0.0 1.565885841812399E-4 8 2.4724513291774725E-5 0.0 1.565885841812399E-4 9 4.120752215295787E-5 0.0 2.225206196259725E-4 10-11 4.944902658354944E-5 0.0 2.225206196259725E-4 12-13 7.417353987532417E-5 0.0 2.225206196259725E-4 14-15 1.0713955759769047E-4 0.0 2.51365885133043E-4 16-17 1.8543384968831042E-4 0.0 2.8021115064011355E-4 18-19 2.925734072860009E-4 4.120752215295787E-6 2.8021115064011355E-4 20-21 4.203167259601703E-4 8.241504430591574E-6 2.8021115064011355E-4 22-23 5.892675667872975E-4 8.241504430591574E-6 2.8021115064011355E-4 24-25 7.953051775520869E-4 8.241504430591574E-6 2.8021115064011355E-4 26-27 9.88980531670989E-4 8.241504430591574E-6 2.8021115064011355E-4 28-29 0.0012650709300958067 8.241504430591574E-6 2.8021115064011355E-4 30-31 0.0015782480984582866 8.241504430591574E-6 3.0493566393188825E-4 32-33 0.0018296139835913296 8.241504430591574E-6 3.0493566393188825E-4 34-35 0.0021304288953079217 1.6483008861183147E-5 3.0493566393188825E-4 36-37 0.0024023985415174438 1.6483008861183147E-5 3.0493566393188825E-4 38-39 0.002773266240894065 1.6483008861183147E-5 3.0493566393188825E-4 40-41 0.0032430319934377847 1.6483008861183147E-5 3.0493566393188825E-4 42-43 0.003659227967182659 1.6483008861183147E-5 3.0493566393188825E-4 44-45 0.00405894093206635 1.6483008861183147E-5 3.0493566393188825E-4 46-47 0.004475136905811225 1.6483008861183147E-5 3.0493566393188825E-4 48-49 0.004850125357403142 1.6483008861183147E-5 3.0493566393188825E-4 50-51 0.005365219384315115 1.6483008861183147E-5 3.3378092943895873E-4 52-53 0.005834985136858834 2.0603761076478938E-5 3.6262619494602927E-4 54-55 0.0063377169071249205 2.4724513291774725E-5 3.7086769937662084E-4 56-57 0.007030003279294613 2.4724513291774725E-5 3.791092038072124E-4 58-59 0.007635753854943094 2.4724513291774725E-5 3.791092038072124E-4 60-61 0.00829507420939042 2.4724513291774725E-5 3.791092038072124E-4 62-63 0.008999722838206 2.4724513291774725E-5 3.791092038072124E-4 64-65 0.009799148767973382 2.4724513291774725E-5 3.791092038072124E-4 66-67 0.010351329564823018 2.4724513291774725E-5 3.791092038072124E-4 68-69 0.010973563149332682 2.4724513291774725E-5 3.791092038072124E-4 70-71 0.01162876275156471 3.2966017722366295E-5 4.2855823039076186E-4 72-73 0.012494120716776826 3.2966017722366295E-5 4.986110180507902E-4 74-75 0.01345013523072545 3.2966017722366295E-5 5.192147791272692E-4 76-77 0.01429076868264579 3.2966017722366295E-5 5.192147791272692E-4 78-79 0.01536628501083799 3.708676993766208E-5 5.192147791272692E-4 80-81 0.016153348683959484 8.241504430591574E-5 5.192147791272692E-4 82-83 0.017072276427970448 1.236225664588736E-4 5.563015490649313E-4 84-85 0.018061256959641434 1.236225664588736E-4 5.851468145720018E-4 86-87 0.01921094682770896 1.236225664588736E-4 6.181128322943681E-4 88-89 0.02041420647457533 1.236225664588736E-4 6.181128322943681E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 1085 0.0 17.946056 3 ATGCCGT 1510 0.0 13.209527 4 CGTATGC 1570 0.0 13.019643 1 TGCCGTC 1650 0.0 12.6643715 5 GCCGTCT 1630 0.0 11.945687 6 GTATGCC 2545 0.0 8.770658 2 CCGTCTT 2865 0.0 7.4593806 7 CGTCTTC 2840 0.0 7.0235195 8 GACACCG 835 7.9449546E-4 6.82511 5 >>END_MODULE