FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n01 2-58885.34100000001acd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n01 2-58885.34100000001acd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12133707
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10850.017.9460563
ATGCCGT15100.013.2095274
CGTATGC15700.013.0196431
TGCCGTC16500.012.66437155
GCCGTCT16300.011.9456876
GTATGCC25450.08.7706582
CCGTCTT28650.07.45938067
CGTCTTC28400.07.02351958
GACACCG8357.9449546E-46.825115
Produced by FastQC (version 0.11.2)