##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n01 12-60303.34100000001c01.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10380212 Sequences flagged as poor quality 0 Sequence length 101 %GC 31 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.92602443957792 38.0 38.0 38.0 32.0 38.0 2 36.92960182316122 38.0 38.0 38.0 32.0 38.0 3 36.90926331755074 38.0 38.0 38.0 32.0 38.0 4 36.894895499244136 38.0 38.0 38.0 32.0 38.0 5 36.8683090480233 38.0 38.0 38.0 32.0 38.0 6 38.78169183827845 40.0 38.0 40.0 38.0 40.0 7 38.72837414110617 40.0 38.0 40.0 38.0 40.0 8 38.710387995929175 40.0 38.0 40.0 38.0 40.0 9 38.70593124687627 40.0 38.0 40.0 38.0 40.0 10-11 38.7204062402579 40.0 38.0 40.0 38.0 40.0 12-13 38.70878764325815 40.0 38.0 40.0 38.0 40.0 14-15 38.686967376003494 40.0 38.0 40.0 38.0 40.0 16-17 38.67007990780921 40.0 38.0 40.0 38.0 40.0 18-19 38.63595464138883 40.0 38.0 40.0 38.0 40.0 20-21 38.60911169251649 40.0 38.0 40.0 38.0 40.0 22-23 38.59080965783743 40.0 38.0 40.0 38.0 40.0 24-25 38.54710761206033 40.0 38.0 40.0 38.0 40.0 26-27 38.471326404508886 40.0 38.0 40.0 38.0 40.0 28-29 38.43457474664294 40.0 38.0 40.0 38.0 40.0 30-31 38.38863623401912 40.0 38.0 40.0 38.0 40.0 32-33 38.3549058535606 40.0 38.0 40.0 38.0 40.0 34-35 38.3235025932033 40.0 38.0 40.0 38.0 40.0 36-37 38.28883726074188 40.0 38.0 40.0 38.0 40.0 38-39 38.23967781197533 40.0 38.0 40.0 38.0 40.0 40-41 38.19945854670406 40.0 38.0 40.0 38.0 40.0 42-43 38.165076011935014 40.0 38.0 40.0 38.0 40.0 44-45 38.13772661868563 40.0 38.0 40.0 38.0 40.0 46-47 38.103429920313765 40.0 38.0 40.0 38.0 40.0 48-49 38.059928544811996 40.0 38.0 40.0 38.0 40.0 50-51 38.00809935288412 40.0 38.0 40.0 38.0 40.0 52-53 37.96749218609408 40.0 38.0 40.0 38.0 40.0 54-55 37.91943806157331 40.0 38.0 40.0 38.0 40.0 56-57 37.86789311239501 40.0 38.0 40.0 35.0 40.0 58-59 37.84357670151631 40.0 38.0 40.0 32.0 40.0 60-61 37.786423051860595 40.0 38.0 40.0 32.0 40.0 62-63 37.75716295582402 40.0 38.0 40.0 32.0 40.0 64-65 37.717519256832134 40.0 38.0 40.0 32.0 40.0 66-67 37.630991014441705 40.0 38.0 40.0 32.0 40.0 68-69 37.57762461884208 40.0 38.0 40.0 32.0 40.0 70-71 37.55868873391025 40.0 38.0 40.0 32.0 40.0 72-73 37.51197624865465 40.0 38.0 40.0 32.0 40.0 74-75 37.44228942530268 40.0 38.0 40.0 32.0 40.0 76-77 37.37639226443545 40.0 38.0 40.0 32.0 40.0 78-79 37.291716970713125 40.0 38.0 40.0 32.0 40.0 80-81 37.249309840685335 38.0 38.0 40.0 32.0 40.0 82-83 37.17844625909375 38.0 38.0 40.0 32.0 40.0 84-85 37.11420628981374 38.0 38.0 40.0 32.0 40.0 86-87 37.05684537078819 38.0 38.0 40.0 32.0 40.0 88-89 37.01121773813483 38.0 38.0 40.0 32.0 40.0 90-91 36.951598676404686 38.0 38.0 40.0 32.0 40.0 92-93 36.8865568448891 38.0 38.0 40.0 32.0 40.0 94-95 36.806137870787225 38.0 38.0 40.0 32.0 40.0 96-97 36.72736250473497 38.0 38.0 40.0 32.0 40.0 98-99 36.62665752876724 38.0 38.0 40.0 32.0 40.0 100-101 35.81596546390382 38.0 35.0 40.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.07937434941835875 2201 2 0.12342868286975062 2201 3 0.0789611277568838 2201 4 -0.007549857740016819 2201 5 0.07342763582746414 2201 6 0.1701227774944627 2201 7 0.21828953966242182 2201 8 0.2270102881076781 2201 9 0.20602301522871613 2201 10-11 0.11212783218444855 2201 12-13 0.04765614159646958 2201 14-15 0.062494909014667144 2201 16-17 0.04138659618694618 2201 18-19 0.05606309472665316 2201 20-21 0.10035326292420876 2201 22-23 0.09360687077720797 2201 24-25 0.04441550242289338 2201 26-27 0.05872359276743566 2201 28-29 0.03793608687832517 2201 30-31 0.06334426590949249 2201 32-33 0.011196130213733113 2201 34-35 0.020314813175332347 2201 36-37 0.07844949961155123 2201 38-39 0.08860294969024096 2201 40-41 0.07098971893746864 2201 42-43 -0.03297634123129001 2201 44-45 -0.015976464092695153 2201 46-47 -0.03265772658876642 2201 48-49 -0.015652143401553076 2201 50-51 -0.03454453048821193 2201 52-53 -0.02124347907020052 2201 54-55 -0.09557793382849411 2201 56-57 -0.09630208834531118 2201 58-59 -0.049851708322776744 2201 60-61 -0.05521821600326149 2201 62-63 -0.1007269495074965 2201 64-65 -0.10041240965505693 2201 66-67 -0.00443095416605388 2201 68-69 -0.036532083268362214 2201 70-71 0.0018629999284272003 2201 72-73 -0.06599437590470814 2201 74-75 -0.018618618511887064 2201 76-77 -0.03942244815171847 2201 78-79 -0.11895808976449018 2201 80-81 -0.09428073922112645 2201 82-83 -0.1329093242168895 2201 84-85 -0.19592612110256624 2201 86-87 -0.15781630079468556 2201 88-89 -0.17625208582504825 2201 90-91 -0.15551213746688575 2201 92-93 -0.21369092075432405 2201 94-95 -0.1405671058108382 2201 96-97 -0.14943107750220008 2201 98-99 -0.2834974922368403 2201 100-101 -0.2833624406315991 1101 1 0.09997357947598573 1101 2 0.08933944980881137 1101 3 0.07315979283261242 1101 4 -0.02260762752993628 1101 5 0.004193181380962585 1101 6 0.09813450855479999 1101 7 0.12281926278205191 1101 8 0.0979304789342379 1101 9 0.06488164434215093 1101 10-11 0.059608125575138615 1101 12-13 0.02483765968751328 1101 14-15 0.04662156671631834 1101 16-17 0.043030611637526306 1101 18-19 0.0719397399464512 1101 20-21 0.03704056054492355 1101 22-23 0.050657466243137605 1101 24-25 0.014935169935171189 1101 26-27 -0.06313573370580627 1101 28-29 -0.012829902950493022 1101 30-31 0.009488243613112957 1101 32-33 -0.02748804944913985 1101 34-35 -0.027616101823682015 1101 36-37 0.032048230803496836 1101 38-39 -0.025943917933133775 1101 40-41 -0.024835367927622087 1101 42-43 -0.005049251144839673 1101 44-45 -0.03223150803484742 1101 46-47 -0.08590889080879549 1101 48-49 -0.11118575118524632 1101 50-51 -0.10028050114504339 1101 52-53 -0.1352617671718903 1101 54-55 -0.09129309319076384 1101 56-57 -0.11164656466950618 1101 58-59 -0.07626798584500705 1101 60-61 -0.11117673212688572 1101 62-63 -0.05204095577854417 1101 64-65 -0.11586884405640063 1101 66-67 -0.13869529636513533 1101 68-69 -0.1503901572618318 1101 70-71 -0.19115289445691985 1101 72-73 -0.1666648933692727 1101 74-75 -0.17108257101007496 1101 76-77 -0.1962981546742668 1101 78-79 -0.21742034020697787 1101 80-81 -0.21780713185012957 1101 82-83 -0.3673683813570605 1101 84-85 -0.2505617798231228 1101 86-87 -0.2078009105756422 1101 88-89 -0.31726461537535755 1101 90-91 -0.2937079749628708 1101 92-93 -0.3186153967901717 1101 94-95 -0.3492686638223077 1101 96-97 -0.3397432043760773 1101 98-99 -0.375057864863372 1101 100-101 -0.4165900939294218 2202 1 0.06952947078639227 2202 2 0.1204310878063879 2202 3 0.042729403602734806 2202 4 0.03109831160422516 2202 5 3.3205350797516076E-4 2202 6 0.060509153012205275 2202 7 0.021251582446943473 2202 8 -0.009679003339428505 2202 9 -0.012789811893313185 2202 10-11 0.029052453839327086 2202 12-13 0.015688317418607767 2202 14-15 -0.01644425244515446 2202 16-17 -0.02105058133157911 2202 18-19 -0.04378451814007178 2202 20-21 -0.05155374084007036 2202 22-23 -0.07991720451184392 2202 24-25 -0.06557922106533454 2202 26-27 -0.007834484120380125 2202 28-29 -0.034709882168726836 2202 30-31 -0.026741879364863053 2202 32-33 -0.07861088433048025 2202 34-35 -0.14778840335056742 2202 36-37 -0.09476011986966171 2202 38-39 -0.08442867327573822 2202 40-41 -0.11075759290405784 2202 42-43 -0.10583113239734132 2202 44-45 -0.14310511248665847 2202 46-47 -0.1954905989314497 2202 48-49 -0.2252921395528915 2202 50-51 -0.22260706434501998 2202 52-53 -0.231836970173795 2202 54-55 -0.21374902311172406 2202 56-57 -0.26123575466545645 2202 58-59 -0.23968481981570733 2202 60-61 -0.2618202455738796 2202 62-63 -0.26034821220560644 2202 64-65 -0.33798347176720966 2202 66-67 -0.24315554477307444 2202 68-69 -0.21157932947696878 2202 70-71 -0.266664465013811 2202 72-73 -0.2979039167980915 2202 74-75 -0.3581331276521027 2202 76-77 -0.39366304491204573 2202 78-79 -0.3284330779809679 2202 80-81 -0.3593125534716748 2202 82-83 -0.3851969676348972 2202 84-85 -0.4123028790913281 2202 86-87 -0.3806315658890824 2202 88-89 -0.3932237346547467 2202 90-91 -0.46848405939336146 2202 92-93 -0.4514098566660678 2202 94-95 -0.5306129468867127 2202 96-97 -0.5345064155748034 2202 98-99 -0.5515023474909384 2202 100-101 -0.5804103456938563 1102 1 0.06675488501423388 1102 2 0.031564328093693916 1102 3 -0.031267602569997166 1102 4 -0.08050882968281314 1102 5 -0.12653598884407558 1102 6 -0.10015477841775322 1102 7 -0.052566088557291835 1102 8 -0.07653738289694445 1102 9 -0.10089131250561678 1102 10-11 -0.10871272335263882 1102 12-13 -0.14814840788103822 1102 14-15 -0.1217889766941056 1102 16-17 -0.11741894275356657 1102 18-19 -0.11972093662254935 1102 20-21 -0.11718754829298206 1102 22-23 -0.11886419193660913 1102 24-25 -0.12186678419621444 1102 26-27 -0.14755265757577263 1102 28-29 -0.18256110039787643 1102 30-31 -0.1765135853533053 1102 32-33 -0.14354704892623715 1102 34-35 -0.22420628494438688 1102 36-37 -0.20738185410014864 1102 38-39 -0.185770493762476 1102 40-41 -0.2695747353650617 1102 42-43 -0.3295683357601362 1102 44-45 -0.30849464581211095 1102 46-47 -0.3333064913170176 1102 48-49 -0.34054774193648996 1102 50-51 -0.31157473652505274 1102 52-53 -0.28443471329685366 1102 54-55 -0.3479504925751229 1102 56-57 -0.4033659194097865 1102 58-59 -0.382148898328154 1102 60-61 -0.3907234744563013 1102 62-63 -0.377833902790762 1102 64-65 -0.49073549333726163 1102 66-67 -0.4241324782465341 1102 68-69 -0.40464936144775976 1102 70-71 -0.5330449028331898 1102 72-73 -0.5069796161706819 1102 74-75 -0.4607222822186685 1102 76-77 -0.5257368104971718 1102 78-79 -0.5730733795851108 1102 80-81 -0.5077356274842089 1102 82-83 -0.6236207712158262 1102 84-85 -0.5599072350391339 1102 86-87 -0.5812265252338946 1102 88-89 -0.5983177842504475 1102 90-91 -0.6337041630685789 1102 92-93 -0.618981441729126 1102 94-95 -0.7341710895062548 1102 96-97 -0.8158115546169427 1102 98-99 -0.9542745113253446 1102 100-101 -0.9843139668704595 2203 1 0.07020427583049837 2203 2 -0.03753962839226688 2203 3 -0.019711868112850084 2203 4 -0.05870812889841659 2203 5 -0.05310088802573176 2203 6 -0.12001620043250227 2203 7 -0.10244972546153974 2203 8 -0.15527151817619256 2203 9 -0.0969342808570346 2203 10-11 -0.1094173409015653 2203 12-13 -0.08579939472445375 2203 14-15 -0.11964312881868722 2203 16-17 -0.15273646305080746 2203 18-19 -0.2490338646846979 2203 20-21 -0.1724079213600973 2203 22-23 -0.12719460199964772 2203 24-25 -0.17602637829801893 2203 26-27 -0.2562899584385079 2203 28-29 -0.21228979911214196 2203 30-31 -0.22001301906485082 2203 32-33 -0.2430157085231457 2203 34-35 -0.2502142299524124 2203 36-37 -0.2403942776511201 2203 38-39 -0.22171237021575507 2203 40-41 -0.2498257173276741 2203 42-43 -0.23017454718481645 2203 44-45 -0.30330739976145793 2203 46-47 -0.31354466282928684 2203 48-49 -0.399664412283407 2203 50-51 -0.35440888481059574 2203 52-53 -0.37358083085245397 2203 54-55 -0.5504378323564367 2203 56-57 -0.3945261858059297 2203 58-59 -0.3962839872505981 2203 60-61 -0.38717035464296146 2203 62-63 -0.45202119570056 2203 64-65 -0.4418436871304081 2203 66-67 -0.4671470827588422 2203 68-69 -0.5445021671393491 2203 70-71 -0.5817897667576446 2203 72-73 -0.5540289088536667 2203 74-75 -0.6152721634455887 2203 76-77 -0.5520338486288452 2203 78-79 -0.5203994507224792 2203 80-81 -0.5589094193391944 2203 82-83 -0.6328715634126496 2203 84-85 -0.6904514589049953 2203 86-87 -0.6934062983190401 2203 88-89 -0.8154108270693214 2203 90-91 -0.7612922959009083 2203 92-93 -0.791694276523593 2203 94-95 -0.8173424843287194 2203 96-97 -0.8677180186694073 2203 98-99 -0.8241942087678922 2203 100-101 -0.8506148285531552 1103 1 0.08364143927584422 1103 2 0.03611430425232243 1103 3 0.03946284921179455 1103 4 0.10567960960194256 1103 5 0.03907262535614109 1103 6 -0.024640867918819254 1103 7 0.017761422366099566 1103 8 -0.03334854240983276 1103 9 -0.015938685671478936 1103 10-11 0.014331472985645632 1103 12-13 -0.008415879566953777 1103 14-15 0.021918511610834912 1103 16-17 -0.012509653504075402 1103 18-19 -0.00725901116300065 1103 20-21 -0.014554356808794466 1103 22-23 6.629632750900782E-4 1103 24-25 0.03510715515631091 1103 26-27 -0.01772759777074384 1103 28-29 -0.08000625274544149 1103 30-31 0.012132484767469975 1103 32-33 -0.05904482976923475 1103 34-35 -0.11717995292320182 1103 36-37 -0.09201263454180264 1103 38-39 -0.02597265321421105 1103 40-41 -0.05849517442509011 1103 42-43 -0.10587277315818255 1103 44-45 -0.11242402036436516 1103 46-47 -0.15229478809219188 1103 48-49 -0.16355415161692122 1103 50-51 -0.25076626933233825 1103 52-53 -0.1423857940563451 1103 54-55 -0.13174946949733624 1103 56-57 -0.08959611987774707 1103 58-59 -0.12496187475768039 1103 60-61 -0.07357975323794363 1103 62-63 -0.15988801406711417 1103 64-65 -0.17546221954250285 1103 66-67 -0.14318499902381632 1103 68-69 -0.188115874131924 1103 70-71 -0.2000792624153931 1103 72-73 -0.17457694489199582 1103 74-75 -0.25308140239062027 1103 76-77 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0.012220524674347644 2204 34-35 0.0254127255184855 2204 36-37 -0.02270167048884275 2204 38-39 0.021909191858561883 2204 40-41 0.06659816392632933 2204 42-43 0.04309748157646709 2204 44-45 -0.003688564655917048 2204 46-47 0.029830906392263046 2204 48-49 0.011335889747087435 2204 50-51 0.05270270365686969 2204 52-53 0.08298547830796821 2204 54-55 0.019497038038025494 2204 56-57 0.024256059080862258 2204 58-59 -0.02255679328527549 2204 60-61 0.004810587626877805 2204 62-63 0.0019837673087792496 2204 64-65 0.023512797035927235 2204 66-67 0.05240921418479161 2204 68-69 0.09056016182626081 2204 70-71 0.026688076745351452 2204 72-73 0.025246084178448314 2204 74-75 0.015637295045074495 2204 76-77 0.007770003131142289 2204 78-79 0.022588606741315687 2204 80-81 0.023949586361233344 2204 82-83 -0.005064908422184544 2204 84-85 0.02046392876609815 2204 86-87 -0.07044946664196772 2204 88-89 -0.036693945470460676 2204 90-91 -0.08831198331372292 2204 92-93 -0.053388394335144085 2204 94-95 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8 -0.035523148476592326 1215 9 -0.061527232700690604 1215 10-11 -0.010421425476039303 1215 12-13 -0.05509369797445629 1215 14-15 -0.05541117633049453 1215 16-17 -0.005383973721770019 1215 18-19 0.018664840379223335 1215 20-21 -0.0016375830622692433 1215 22-23 -0.03625970451151517 1215 24-25 0.008409214618815497 1215 26-27 0.0496273833105505 1215 28-29 0.05125947106986217 1215 30-31 0.006847166899419221 1215 32-33 0.034916422687715 1215 34-35 0.054998856768257554 1215 36-37 0.04709984556006219 1215 38-39 0.07066414078986583 1215 40-41 0.07386541773054489 1215 42-43 0.0968663660375455 1215 44-45 0.09937946524427588 1215 46-47 0.13936463164009183 1215 48-49 0.1812051650880946 1215 50-51 0.1512168335610582 1215 52-53 0.1667912774592608 1215 54-55 0.2147753935675638 1215 56-57 0.1616691984621923 1215 58-59 0.17127208371547908 1215 60-61 0.20983139920512883 1215 62-63 0.1861266805592905 1215 64-65 0.27377800762037197 1215 66-67 0.3422750307625648 1215 68-69 0.3388735972888739 1215 70-71 0.2751003084739736 1215 72-73 0.3076582570512443 1215 74-75 0.2968479115208993 1215 76-77 0.3001739236730572 1215 78-79 0.34450798684284223 1215 80-81 0.34740077163752403 1215 82-83 0.3944225917583921 1215 84-85 0.297173826457076 1215 86-87 0.4267787563797114 1215 88-89 0.3883393996102029 1215 90-91 0.41292496703326975 1215 92-93 0.4647972163516485 1215 94-95 0.4424770300276819 1215 96-97 0.4958991501147807 1215 98-99 0.5555446222799674 1215 100-101 0.6958036576785886 1212 1 -0.1902485364022084 1212 2 -0.13798071121656363 1212 3 -0.12348464573205575 1212 4 -0.08218616951997859 1212 5 -0.04890973048484426 1212 6 -0.08711943751738005 1212 7 -0.1511224242904845 1212 8 -0.20347877303267126 1212 9 -0.28141448355953 1212 10-11 -0.22878804593413804 1212 12-13 -0.20505680254677827 1212 14-15 -0.194259652418026 1212 16-17 -0.087560562500407 1212 18-19 -0.0340816111039004 1212 20-21 -0.007651097599129741 1212 22-23 0.028772911553929248 1212 24-25 -0.016400473847028252 1212 26-27 0.0195158565423057 1212 28-29 0.03907521871519748 1212 30-31 0.0032688049507001438 1212 32-33 -0.04791357764464976 1212 34-35 0.015013360951535049 1212 36-37 -0.09473840066293349 1212 38-39 -0.12315157336290383 1212 40-41 -0.18826485013613592 1212 42-43 -0.2658921109072452 1212 44-45 -0.05219004723792153 1212 46-47 -0.06188463952288714 1212 48-49 -0.17922261288422447 1212 50-51 -0.08764027388362905 1212 52-53 -0.1627899961681507 1212 54-55 -0.23347825381127052 1212 56-57 -0.1618659997546743 1212 58-59 -0.20575106842021285 1212 60-61 -0.20851085231639388 1212 62-63 -0.13428286555861746 1212 64-65 0.07967118146869012 1212 66-67 0.04452705391810241 1212 68-69 -0.16396863341682177 1212 70-71 -0.022157348091724316 1212 72-73 -0.018508066029724546 1212 74-75 0.04951700846726936 1212 76-77 -0.010941731635178087 1212 78-79 0.17421150078219227 1212 80-81 0.20309253180061404 1212 82-83 0.2140082404087309 1212 84-85 0.28679884155553026 1212 86-87 0.1929487319321055 1212 88-89 0.19801985471889338 1212 90-91 0.29945826956794264 1212 92-93 0.3261978249831188 1212 94-95 0.2349182292469365 1212 96-97 0.2863731428436296 1212 98-99 0.28780549740868366 1212 100-101 0.3876877946047088 1213 1 -0.3643437071884392 1213 2 -0.23859924918495778 1213 3 -0.17042191435673715 1213 4 -0.18531679265279877 1213 5 -0.11794462259119598 1213 6 -0.07291809922166692 1213 7 -0.06472062338007589 1213 8 -0.07701248606064581 1213 9 -0.09235384716183859 1213 10-11 -0.13091534231563173 1213 12-13 -0.13366156021550069 1213 14-15 -0.2213094846924406 1213 16-17 -0.3751781113810111 1213 18-19 -0.31377986101976774 1213 20-21 -0.17312222147276657 1213 22-23 -0.24682425160331434 1213 24-25 -0.32908654018936545 1213 26-27 -0.19555512019233845 1213 28-29 -0.09024583360496052 1213 30-31 -0.16420747487967446 1213 32-33 -0.14634156619577254 1213 34-35 -0.010575058627459555 1213 36-37 -0.01814909098988693 1213 38-39 0.020006965991257175 1213 40-41 -0.008978176988435393 1213 42-43 0.04872978291974306 1213 44-45 0.044355939932692934 1213 46-47 0.06780315909732337 1213 48-49 0.11151250895939313 1213 50-51 0.12198063034643525 1213 52-53 0.11836230748061638 1213 54-55 0.13724527834722977 1213 56-57 0.1288683302456377 1213 58-59 0.1323708862780677 1213 60-61 0.1582905052695125 1213 62-63 0.19275996238067705 1213 64-65 0.20746068449714272 1213 66-67 0.2383036574667443 1213 68-69 0.1974684045949644 1213 70-71 0.185551711885509 1213 72-73 0.12623826273232908 1213 74-75 0.13736404922848777 1213 76-77 0.22989723254034544 1213 78-79 0.2446859773500023 1213 80-81 0.23990488481914696 1213 82-83 0.3573273493056206 1213 84-85 0.30361904693636177 1213 86-87 0.3008571614783264 1213 88-89 0.3275848140201134 1213 90-91 0.38720457305909406 1213 92-93 0.415312008933725 1213 94-95 0.44423771795036515 1213 96-97 0.4180855516046691 1213 98-99 0.4082176183138131 1213 100-101 0.37558742826661984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 11.0 14 28.0 15 86.0 16 171.0 17 338.0 18 774.0 19 1621.0 20 3377.0 21 6187.0 22 10866.0 23 17116.0 24 25835.0 25 36739.0 26 49900.0 27 66089.0 28 84966.0 29 105497.0 30 129470.0 31 156797.0 32 188915.0 33 229297.0 34 284557.0 35 367760.0 36 510654.0 37 816085.0 38 1772493.0 39 5514583.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.939700469517069 34.72001103157789 35.5219508149771 15.818337683927936 2 14.574377966596947 36.18557278075259 34.45725985744246 14.782789395208013 3 14.703350952755107 34.65292423700017 34.88722581003162 15.756499000213097 4 15.172753697130656 34.36600331476852 35.01908246189962 15.4421605262012 5 15.527062453059726 34.58812787253285 34.803470295211696 15.081339379195724 6 15.298714515657291 35.02771427019024 33.935838689999784 15.737732524152687 7 15.382922815063893 34.759328614868366 34.65261595813265 15.205132611935094 8 15.09112723323955 35.010903438195676 34.53769537654915 15.360273952015625 9 15.058008493860042 34.381597895378555 34.953792815632525 15.606600795128884 10-11 15.108001238896534 34.754506293469156 34.73444406926453 15.403048398369782 12-13 14.90523989298099 34.66644033859809 34.959083687308116 15.469236081112795 14-15 14.958810529398617 34.549598847022374 34.90234179312684 15.589248830452174 16-17 15.068117105893405 34.4450238588576 34.989940475204165 15.496918560044822 18-19 15.11637719923254 34.28360133685131 34.89429213969811 15.70572932421804 20-21 15.219116474506725 34.170483348656575 34.765881403905716 15.844518772930979 22-23 15.188521951943686 34.23770281045726 34.78058268065625 15.7931925569428 24-25 15.310145375715095 34.13469284831249 34.682366791110645 15.872794984861763 26-27 15.40689516444852 34.16519774409716 34.57221863039866 15.855688461055657 28-29 15.39075217108089 34.11420328399001 34.57263183023972 15.922412714689383 30-31 15.424944005534586 34.00497147325428 34.55435958646876 16.015724934742366 32-33 15.513266059893866 34.13946975260308 34.43633064388904 15.910933543614014 34-35 15.492160816476863 34.12231208260456 34.47923264631881 15.906294454599761 36-37 15.490286310956265 33.966318131944966 34.448012748974506 16.095382808124263 38-39 15.600126909336614 34.02721420999179 34.4430417273698 15.929617153301795 40-41 15.596375886124052 34.10116419453032 34.36592049978714 15.936539419558486 42-43 15.50685017825364 33.91706945939934 34.43672037969762 16.1393599826494 44-45 15.592478744674304 33.99375046549696 34.37590841158901 16.037862378239726 46-47 15.663807212960974 34.06070303577807 34.34302884469469 15.932460906566268 48-49 15.547890693576067 33.88374846919554 34.38919577242635 16.17916506480205 50-51 15.681786243580781 33.87255808087268 34.303527422735606 16.142128252810938 52-53 15.647916118263213 34.02635150056674 34.33712523408718 15.988607147082867 54-55 15.635632040985248 33.852806315633174 34.35077021979005 16.160791423591522 56-57 15.735133565917922 33.90050596734688 34.26033794298399 16.104022523751212 58-59 15.71039978919037 33.8892232970655 34.393624839309446 16.006752074434683 60-61 15.742472505880054 33.88652029662463 34.21541115861784 16.155596038877476 62-63 15.678052991737317 33.851215866181676 34.2680903270067 16.2026408150743 64-65 15.674959107152025 33.91580413320505 34.29570613294937 16.113530626693553 66-67 15.744569219941384 33.881904711991595 34.23825099423826 16.135275073828762 68-69 15.728342549952792 33.89922734542091 34.25450875739417 16.117921347232123 70-71 15.770081274150858 33.92183977987259 34.203131137216715 16.104947808759835 72-73 15.734524288481078 33.82787417599076 34.224585688313624 16.213015847214542 74-75 15.772627829654464 33.908955727124145 34.182389250847834 16.136027192373557 76-77 15.764040229290224 33.93191855074023 34.19422210375188 16.109819116217672 78-79 15.79212084142069 33.820204012163735 34.22165647200078 16.16601867441479 80-81 15.84982845046069 33.901204905765184 34.175896151523425 16.0730704922507 82-83 15.76855228598077 33.997970788196575 34.13718337171894 16.096293554103713 84-85 15.757094804087174 33.75024713353998 34.220663177314506 16.271994885058337 86-87 15.77417797191102 33.95121969549996 34.1568793763023 16.11772295628673 88-89 15.815624490576116 33.96857247240007 34.15376190389939 16.06204113312443 90-91 15.756043761770375 33.791701661165895 34.19690966156475 16.255344915498974 92-93 15.810918071396241 33.84487765811795 34.18101264627562 16.1631916242102 94-95 15.796496316691764 33.926367943112 34.139802014655 16.137333725541243 96-97 15.78823134739404 33.8671010646933 34.15022379379351 16.194443794119152 98-99 15.822874874823182 33.863660406203955 34.1389048875851 16.174559831387768 100-101 15.837211003934904 33.959261566212994 34.09260388473062 16.11092354512148 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1102.0 1 1036.5 2 1021.0 3 1213.5 4 1580.0 5 2036.5 6 2665.0 7 3539.5 8 4840.5 9 6878.0 10 9902.5 11 14140.5 12 20727.0 13 30999.0 14 45888.0 15 66785.0 16 94763.0 17 128697.0 18 168714.5 19 213447.0 20 259919.5 21 303998.5 22 346004.5 23 382864.5 24 410314.5 25 428255.5 26 435625.5 27 435844.5 28 436624.5 29 438072.0 30 435011.0 31 430969.0 32 422030.0 33 400039.0 34 370088.5 35 336802.0 36 305070.0 37 280597.5 38 258689.0 39 239155.5 40 223746.0 41 208107.0 42 191490.0 43 175126.5 44 157485.5 45 141916.5 46 130152.0 47 124394.0 48 116650.0 49 103103.5 50 89148.0 51 75895.5 52 64942.0 53 56220.5 54 50007.0 55 44688.5 56 40241.5 57 35853.5 58 32262.5 59 28522.0 60 23978.0 61 19740.0 62 15635.5 63 12372.0 64 9799.0 65 7742.0 66 6202.5 67 4742.0 68 3525.5 69 2628.0 70 1943.5 71 1459.0 72 1125.5 73 870.0 74 706.5 75 559.5 76 423.5 77 340.0 78 272.0 79 216.0 80 180.5 81 136.5 82 108.0 83 76.5 84 36.0 85 16.5 86 7.5 87 3.5 88 4.0 89 2.5 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.5 97 1.0 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09616373923769572 2 0.0025336669424478033 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 7.706971688054156E-5 10-11 6.743600227047386E-5 12-13 0.0 14-15 0.0012716503285289356 16-17 0.0 18-19 0.0 20-21 1.3005514723591385E-4 22-23 1.4450571915101542E-4 24-25 3.8053172709767393E-4 26-27 0.0014932257645604925 28-29 0.001796687774777625 30-31 0.003771599269841502 32-33 0.004185848998074414 34-35 0.005698342191855041 36-37 0.006844754230453097 38-39 0.011069138086967781 40-41 0.0118831869715185 42-43 0.012730953857204458 44-45 0.014633612492692826 46-47 0.01370877589012633 48-49 0.01620390797413386 50-51 0.01459989449155759 52-53 0.015413943376108312 54-55 0.015881178534696595 56-57 0.014248263908290119 58-59 0.011362966382574845 60-61 0.0060596064897325795 62-63 0.0033573495416085912 64-65 3.371800113523693E-4 66-67 0.0027022569481239883 68-69 0.00396909041934789 70-71 0.0039016544170774165 72-73 0.01698905571485438 74-75 0.012258901841311141 76-77 0.033660198847576525 78-79 0.025476358286324018 80-81 0.01940711808198137 82-83 0.031564865919886796 84-85 0.011637527248961775 86-87 0.01597751568079727 88-89 0.008092320272456863 90-91 0.005823580481785921 92-93 0.012966979865151117 94-95 0.013130753013522267 96-97 0.007620268256563546 98-99 0.008612540861400519 100-101 0.0015558449095259325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0380212E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.37269585327546 #Duplication Level Percentage of deduplicated Percentage of total 1 45.456797104646284 19.71583835283876 2 25.025291040072617 21.70828673841538 3 13.954699421154748 18.15758801152872 4 7.308885323790655 12.680242407009631 5 3.515383163324109 7.623582237529096 6 1.7775876754914506 4.6259261760972965 7 0.9268996805193888 2.814149655076595 8 0.48320424579139265 1.6766296630177142 9 0.317450685891899 1.2391822842842717 >10 1.2008738242052235 8.76540384296681 >50 0.03162607697576204 0.8697765928475043 >100 0.0013017581364410547 0.12339403838819826 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.890114383020308E-5 0.0 0.0 2 2.890114383020308E-5 0.0 0.0 3 2.890114383020308E-5 0.0 0.0 4 2.890114383020308E-5 0.0 0.0 5 2.890114383020308E-5 0.0 3.853485844027078E-5 6 2.890114383020308E-5 0.0 3.853485844027078E-5 7 2.890114383020308E-5 0.0 3.853485844027078E-5 8 3.853485844027078E-5 0.0 3.853485844027078E-5 9 3.853485844027078E-5 0.0 3.853485844027078E-5 10-11 3.853485844027078E-5 0.0 4.3351715745304624E-5 12-13 3.853485844027078E-5 0.0 7.706971688054156E-5 14-15 5.298543035537232E-5 0.0 7.706971688054156E-5 16-17 8.18865741855754E-5 0.0 8.670343149060925E-5 18-19 1.3487200454094772E-4 0.0 8.670343149060925E-5 20-21 1.6859000567618466E-4 0.0 8.670343149060925E-5 22-23 1.9749114950638774E-4 0.0 9.633714610067694E-5 24-25 2.6974400908189543E-4 0.0 1.2523828993088E-4 26-27 3.5644744057250466E-4 0.0 1.2523828993088E-4 28-29 4.4796772936814776E-4 1.926742922013539E-5 1.2523828993088E-4 30-31 5.105868743335878E-4 1.926742922013539E-5 1.2523828993088E-4 32-33 6.59909450789637E-4 2.890114383020308E-5 1.3968886184598158E-4 34-35 7.225285957550771E-4 2.890114383020308E-5 1.637731483711508E-4 36-37 7.610634541953479E-4 2.890114383020308E-5 1.734068629812185E-4 38-39 8.18865741855754E-4 3.853485844027078E-5 2.0230800681142157E-4 40-41 9.200197452614647E-4 3.853485844027078E-5 2.0230800681142157E-4 42-43 0.0010597086071074463 3.853485844027078E-5 2.071248641164554E-4 44-45 0.0012475660420037662 3.853485844027078E-5 2.1194172142148927E-4 46-47 0.0014450571915101541 4.816857305033847E-5 2.4084286525169235E-4 48-49 0.001632914626406474 4.816857305033847E-5 2.5047657986176E-4 50-51 0.0018496732051329974 4.816857305033847E-5 2.5047657986176E-4 52-53 0.002109783499604825 4.816857305033847E-5 2.5047657986176E-4 54-55 0.0023265420783313484 4.816857305033847E-5 2.6974400908189543E-4 56-57 0.002596286087413244 4.816857305033847E-5 2.793777236919631E-4 58-59 0.0028901143830203082 4.816857305033847E-5 2.793777236919631E-4 60-61 0.0032224775370676435 6.261914496544002E-5 2.793777236919631E-4 62-63 0.0035211226899797424 7.706971688054156E-5 3.0346201021713237E-4 64-65 0.0038005004136717055 7.706971688054156E-5 3.660811551825724E-4 66-67 0.004113596138498906 7.706971688054156E-5 3.7089801248760626E-4 68-69 0.004523029009426782 1.0597086071074464E-4 3.7571486979264007E-4 70-71 0.004922828165744591 1.2523828993088E-4 4.142497282329109E-4 72-73 0.0053322610366724685 1.2523828993088E-4 4.2388344284297855E-4 74-75 0.005707975906465108 1.4450571915101542E-4 4.8650258780841857E-4 76-77 0.00612222563469802 1.4450571915101542E-4 5.105868743335878E-4 78-79 0.0067339665124373175 1.4450571915101542E-4 5.250374462486894E-4 80-81 0.007177117384500432 1.4450571915101542E-4 5.587554473839262E-4 82-83 0.007591367112733343 1.4932257645604925E-4 6.021071631292309E-4 84-85 0.008116404558982031 1.5413943376108312E-4 6.502757361795694E-4 86-87 0.008679976863670993 1.5413943376108312E-4 6.647263080946709E-4 88-89 0.009416956031341172 1.5413943376108312E-4 6.984443092299078E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 785 0.0 16.334593 6 TGCCGTC 845 0.0 15.174742 5 TATGCCG 905 0.0 13.643915 3 ATGCCGT 1050 0.0 12.212053 4 CGTATGC 1465 0.0 9.410486 1 GTATGCC 1980 3.6379788E-12 6.9559674 2 TCCCGGC 495 0.0053784302 5.757176 42-43 CCGTCTT 2460 1.1186785E-9 5.5985703 7 >>END_MODULE