FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n01 12-60303.34100000001c01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n01 12-60303.34100000001c01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10380212
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT7850.016.3345936
TGCCGTC8450.015.1747425
TATGCCG9050.013.6439153
ATGCCGT10500.012.2120534
CGTATGC14650.09.4104861
GTATGCC19803.6379788E-126.95596742
TCCCGGC4950.00537843025.75717642-43
CCGTCTT24601.1186785E-95.59857037
Produced by FastQC (version 0.11.2)