##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n01 11-60308.34100000001be6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10132962 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.89783717732288 38.0 38.0 38.0 32.0 38.0 2 36.897072050600805 38.0 38.0 38.0 32.0 38.0 3 36.87922248203437 38.0 38.0 38.0 32.0 38.0 4 36.87159263007204 38.0 38.0 38.0 32.0 38.0 5 36.84911628011632 38.0 38.0 38.0 32.0 38.0 6 38.770832950918006 40.0 38.0 40.0 38.0 40.0 7 38.7171144034686 40.0 38.0 40.0 38.0 40.0 8 38.70593040810772 40.0 38.0 40.0 38.0 40.0 9 38.703059283159256 40.0 38.0 40.0 38.0 40.0 10-11 38.71715629645112 40.0 38.0 40.0 38.0 40.0 12-13 38.70723960081958 40.0 38.0 40.0 38.0 40.0 14-15 38.690435876498896 40.0 38.0 40.0 38.0 40.0 16-17 38.66957016122235 40.0 38.0 40.0 38.0 40.0 18-19 38.63752558235193 40.0 38.0 40.0 38.0 40.0 20-21 38.61149568112463 40.0 38.0 40.0 38.0 40.0 22-23 38.59260599220643 40.0 38.0 40.0 38.0 40.0 24-25 38.549333797955626 40.0 38.0 40.0 38.0 40.0 26-27 38.473511200377544 40.0 38.0 40.0 38.0 40.0 28-29 38.43490758181073 40.0 38.0 40.0 38.0 40.0 30-31 38.38681359902465 40.0 38.0 40.0 38.0 40.0 32-33 38.351419851372185 40.0 38.0 40.0 38.0 40.0 34-35 38.320365358125294 40.0 38.0 40.0 38.0 40.0 36-37 38.28285796393986 40.0 38.0 40.0 38.0 40.0 38-39 38.23438975691411 40.0 38.0 40.0 38.0 40.0 40-41 38.19343164417275 40.0 38.0 40.0 38.0 40.0 42-43 38.156302076332665 40.0 38.0 40.0 38.0 40.0 44-45 38.12800200967891 40.0 38.0 40.0 38.0 40.0 46-47 38.093999266946824 40.0 38.0 40.0 38.0 40.0 48-49 38.05004726160031 40.0 38.0 40.0 38.0 40.0 50-51 37.99810317062277 40.0 38.0 40.0 38.0 40.0 52-53 37.95364543950723 40.0 38.0 40.0 38.0 40.0 54-55 37.90331716431977 40.0 38.0 40.0 38.0 40.0 56-57 37.851340358327604 40.0 38.0 40.0 32.0 40.0 58-59 37.827249327491806 40.0 38.0 40.0 32.0 40.0 60-61 37.76814770449154 40.0 38.0 40.0 32.0 40.0 62-63 37.73543254183723 40.0 38.0 40.0 32.0 40.0 64-65 37.693686110734454 40.0 38.0 40.0 32.0 40.0 66-67 37.60656859267803 40.0 38.0 40.0 32.0 40.0 68-69 37.550752928906675 40.0 38.0 40.0 32.0 40.0 70-71 37.53105395046384 40.0 38.0 40.0 32.0 40.0 72-73 37.48360331362143 40.0 38.0 40.0 32.0 40.0 74-75 37.415520999684006 40.0 38.0 40.0 32.0 40.0 76-77 37.34537068233355 40.0 38.0 40.0 32.0 40.0 78-79 37.26074735107069 39.0 38.0 40.0 32.0 40.0 80-81 37.21886038850239 38.0 38.0 40.0 32.0 40.0 82-83 37.14606568148583 38.0 38.0 40.0 32.0 40.0 84-85 37.07738556603686 38.0 38.0 40.0 32.0 40.0 86-87 37.02042808410808 38.0 38.0 40.0 32.0 40.0 88-89 36.97509765653913 38.0 38.0 40.0 32.0 40.0 90-91 36.91297209049043 38.0 38.0 40.0 32.0 40.0 92-93 36.84639955227307 38.0 38.0 40.0 32.0 40.0 94-95 36.76473789203986 38.0 38.0 40.0 32.0 40.0 96-97 36.681187050736 38.0 38.0 40.0 32.0 40.0 98-99 36.57895371560655 38.0 38.0 40.0 32.0 40.0 100-101 35.74341836079125 38.0 35.0 39.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.05991304286275323 2201 2 0.09072288280879803 2201 3 0.10698582378599042 2201 4 0.008015264525717214 2201 5 0.10974965178334628 2201 6 0.17930889673306893 2201 7 0.20090307514728778 2201 8 0.1843236628528757 2201 9 0.16597256186008735 2201 10-11 0.13807297804817154 2201 12-13 0.1015275587540927 2201 14-15 0.08995908215631943 2201 16-17 0.08375219221949237 2201 18-19 0.0545082867366844 2201 20-21 0.10374150786992686 2201 22-23 0.1311524585328101 2201 24-25 0.04616447635666532 2201 26-27 0.04835546461994511 2201 28-29 0.0685485400148167 2201 30-31 0.05887000112419116 2201 32-33 0.05888065130289277 2201 34-35 0.04673748651627818 2201 36-37 0.079927967514692 2201 38-39 0.0592928217887021 2201 40-41 0.05165852011210603 2201 42-43 -0.008725548012151307 2201 44-45 -0.050052715012093074 2201 46-47 -0.02139405814006068 2201 48-49 -0.035400112381424265 2201 50-51 -0.10453100890842393 2201 52-53 -0.0839942598851735 2201 54-55 -0.1570727641455889 2201 56-57 -0.1102302531989352 2201 58-59 -0.026317931869897393 2201 60-61 -0.04178570987949115 2201 62-63 -0.06092439690992535 2201 64-65 -0.09894833404482029 2201 66-67 -0.04783936907101349 2201 68-69 -0.11488056150322734 2201 70-71 -0.06018648624008449 2201 72-73 -0.07093905085834962 2201 74-75 -0.07236377035045649 2201 76-77 -0.12221178330446492 2201 78-79 -0.057006249613557713 2201 80-81 -0.11995675224036972 2201 82-83 -0.1769192105807207 2201 84-85 -0.20379495656187174 2201 86-87 -0.22585106460208948 2201 88-89 -0.24098682301617913 2201 90-91 -0.23612349199429872 2201 92-93 -0.24108527288664305 2201 94-95 -0.2686853331941279 2201 96-97 -0.19202766599823917 2201 98-99 -0.2148091604993283 2201 100-101 -0.34983321862213757 1101 1 0.10436528702762615 1101 2 0.11691909499074171 1101 3 0.10187678475227102 1101 4 0.04780213214583284 1101 5 0.03969419099323801 1101 6 0.12946659215472067 1101 7 0.16060815313727517 1101 8 0.0676236370949681 1101 9 0.1152715362861585 1101 10-11 0.10874187424048642 1101 12-13 0.05455582678714421 1101 14-15 0.05853476321208717 1101 16-17 0.04727315201812132 1101 18-19 0.07188716525593719 1101 20-21 0.02752605946689357 1101 22-23 0.05796366721954627 1101 24-25 0.07227783552551159 1101 26-27 0.03288594918377896 1101 28-29 0.04365283226550076 1101 30-31 0.0715913601709417 1101 32-33 -0.009409099350008887 1101 34-35 0.011042990590453883 1101 36-37 0.00740355567999984 1101 38-39 0.01794928569233889 1101 40-41 0.03713015761659477 1101 42-43 0.01884630776368823 1101 44-45 0.02193508141650824 1101 46-47 -0.03093534524731467 1101 48-49 -0.05553927519527946 1101 50-51 -0.006101869955429606 1101 52-53 -0.05709810659431014 1101 54-55 -0.056919970299595946 1101 56-57 -0.04719605606963739 1101 58-59 -0.0262745876419217 1101 60-61 -0.031050422772906927 1101 62-63 -0.0010201932679905212 1101 64-65 -0.05350330884220966 1101 66-67 -0.05358502000822796 1101 68-69 -0.06506180809078188 1101 70-71 -0.08722904517331642 1101 72-73 -0.09414913446504158 1101 74-75 -0.12529769680117653 1101 76-77 -0.13228652637533855 1101 78-79 -0.12436250388947201 1101 80-81 -0.14185976619037888 1101 82-83 -0.2726094709631184 1101 84-85 -0.13982364530777147 1101 86-87 -0.16848190249986317 1101 88-89 -0.2654450811783633 1101 90-91 -0.2964615962213415 1101 92-93 -0.2759501158333748 1101 94-95 -0.2737465050989982 1101 96-97 -0.2591589754177477 1101 98-99 -0.2914709954437029 1101 100-101 -0.36280891994876185 2202 1 0.09057588511073078 2202 2 0.10863167837814558 2202 3 0.03372866943590225 2202 4 0.05638069875787011 2202 5 0.03435440874729068 2202 6 0.08039170236846616 2202 7 0.06487018952011425 2202 8 0.03539849293566988 2202 9 0.028663871765211013 2202 10-11 0.09444357619508281 2202 12-13 0.048988327712756075 2202 14-15 0.004764750582133104 2202 16-17 -0.010485759516853932 2202 18-19 -0.032581522029055066 2202 20-21 -0.03465921505906522 2202 22-23 -0.020927671669277004 2202 24-25 -0.0388796236107396 2202 26-27 -0.029689122512664312 2202 28-29 -0.035411572863218055 2202 30-31 -0.04130730102099989 2202 32-33 -0.07862531502832582 2202 34-35 -0.07679037548953715 2202 36-37 -0.07135339212643999 2202 38-39 -0.04539008264269029 2202 40-41 -0.07712582394400869 2202 42-43 -0.14312732409571538 2202 44-45 -0.14704659384929641 2202 46-47 -0.1111236476083235 2202 48-49 -0.15591548822916934 2202 50-51 -0.24952810558920646 2202 52-53 -0.19284795126894494 2202 54-55 -0.1790965053053526 2202 56-57 -0.2676834832046069 2202 58-59 -0.24316662521042076 2202 60-61 -0.17251125255553745 2202 62-63 -0.2759280991473645 2202 64-65 -0.305604953771649 2202 66-67 -0.19957643238849698 2202 68-69 -0.2892674275512874 2202 70-71 -0.21355973610490508 2202 72-73 -0.2405556403076119 2202 74-75 -0.2557354791685924 2202 76-77 -0.311265028420749 2202 78-79 -0.2790352131588065 2202 80-81 -0.3375061232052943 2202 82-83 -0.3414444796119369 2202 84-85 -0.3941586990046346 2202 86-87 -0.3860339166960429 2202 88-89 -0.3326359117405744 2202 90-91 -0.4226420628788574 2202 92-93 -0.4484422749311747 2202 94-95 -0.44534660036701723 2202 96-97 -0.5351589615396435 2202 98-99 -0.5177133735900981 2202 100-101 -0.5460635467660424 1102 1 0.09717586690259594 1102 2 0.030787780042977886 1102 3 0.04537094010271403 1102 4 -0.06441825980188298 1102 5 -0.12171564779590938 1102 6 -0.04561072264392152 1102 7 0.0014404306271842415 1102 8 -0.0103307483304107 1102 9 -0.0692259069817922 1102 10-11 -0.0474327668690151 1102 12-13 -0.08664898598285475 1102 14-15 -0.06359460854163501 1102 16-17 -0.06633584156550398 1102 18-19 -0.1160252729328235 1102 20-21 -0.08927052939327496 1102 22-23 -0.10351544748637309 1102 24-25 -0.08898902455301538 1102 26-27 -0.11422119828961286 1102 28-29 -0.16775807058089498 1102 30-31 -0.13875306975862856 1102 32-33 -0.10343881346865658 1102 34-35 -0.154740874233795 1102 36-37 -0.19272590526505695 1102 38-39 -0.17466796985674193 1102 40-41 -0.2639566700699376 1102 42-43 -0.28962093846263315 1102 44-45 -0.3057803177479883 1102 46-47 -0.32705073734432233 1102 48-49 -0.2810995825056537 1102 50-51 -0.3226609155836755 1102 52-53 -0.28394501453065857 1102 54-55 -0.31174332574287433 1102 56-57 -0.39237860460659846 1102 58-59 -0.34902307505309693 1102 60-61 -0.3590029535085506 1102 62-63 -0.36234167846098586 1102 64-65 -0.4359844270618254 1102 66-67 -0.4036697974744641 1102 68-69 -0.4205728398269031 1102 70-71 -0.5069410057347241 1102 72-73 -0.5074858740004871 1102 74-75 -0.5057011137319591 1102 76-77 -0.4610400559163992 1102 78-79 -0.45265375720757106 1102 80-81 -0.4509244053495607 1102 82-83 -0.5352957939391274 1102 84-85 -0.5677829138722146 1102 86-87 -0.6300270009443878 1102 88-89 -0.6380379743873661 1102 90-91 -0.6619612527671563 1102 92-93 -0.719325983809469 1102 94-95 -0.6782885219772155 1102 96-97 -0.8003146902980234 1102 98-99 -0.8989105734611513 1102 100-101 -0.8991404358412893 2203 1 0.10941686947992224 2203 2 0.014947825013052807 2203 3 0.00827638320315316 2203 4 -0.01830210172168023 2203 5 -0.0044327261232837145 2203 6 -0.1357924651442417 2203 7 -0.05454429608868594 2203 8 -0.0900136167200003 2203 9 -0.09769723132523467 2203 10-11 -0.10921526633617162 2203 12-13 -0.11514597210886279 2203 14-15 -0.10507933475101794 2203 16-17 -0.11069289999394982 2203 18-19 -0.17048804102267212 2203 20-21 -0.11604049309573838 2203 22-23 -0.07046586658031373 2203 24-25 -0.11265780899741173 2203 26-27 -0.18827405488362103 2203 28-29 -0.13548681936495655 2203 30-31 -0.16758721783479302 2203 32-33 -0.13015496139027505 2203 34-35 -0.17324170099822567 2203 36-37 -0.18668011417229735 2203 38-39 -0.1661078680727215 2203 40-41 -0.14346811314275243 2203 42-43 -0.1306492075905652 2203 44-45 -0.22507880307735206 2203 46-47 -0.22960416188750088 2203 48-49 -0.29144638054092553 2203 50-51 -0.2617453748933727 2203 52-53 -0.20469929417824062 2203 54-55 -0.35210063787353363 2203 56-57 -0.2924654403699378 2203 58-59 -0.23472326592841597 2203 60-61 -0.21106282527713205 2203 62-63 -0.3311630092592708 2203 64-65 -0.3053028659278212 2203 66-67 -0.35888063707572826 2203 68-69 -0.39655700228369284 2203 70-71 -0.4367501616625802 2203 72-73 -0.4413676266395683 2203 74-75 -0.5275787909712477 2203 76-77 -0.43443689380059425 2203 78-79 -0.38743055026715467 2203 80-81 -0.507765502945162 2203 82-83 -0.49373141597921233 2203 84-85 -0.5767372895435301 2203 86-87 -0.5571607096954523 2203 88-89 -0.6922847906447558 2203 90-91 -0.6395858854071932 2203 92-93 -0.6992990286473386 2203 94-95 -0.6599828732212814 2203 96-97 -0.7306240955573315 2203 98-99 -0.6789435751137987 2203 100-101 -0.747259144460422 1103 1 0.06800939297007602 1103 2 0.055101674458988725 1103 3 0.08709718338095485 1103 4 0.07979119508875243 1103 5 -0.023901576313178907 1103 6 0.03696441942601325 1103 7 0.03622937927609371 1103 8 0.014921605948273964 1103 9 -0.035577757963231704 1103 10-11 0.021892703097655897 1103 12-13 0.018323760165380065 1103 14-15 0.019746659532167143 1103 16-17 0.01469265560802313 1103 18-19 0.006877927212514123 1103 20-21 -0.014180179044878116 1103 22-23 -0.017703630744463794 1103 24-25 0.017152389961431425 1103 26-27 -0.013055029250082839 1103 28-29 -0.08311356218602128 1103 30-31 -0.013245976893344391 1103 32-33 -0.0791495315140196 1103 34-35 -0.05943498120696944 1103 36-37 -0.07706840546959626 1103 38-39 -0.029536336475885605 1103 40-41 -0.05939261823365172 1103 42-43 -0.10031916951133724 1103 44-45 -0.061120157164381794 1103 46-47 -0.11134255623987599 1103 48-49 -0.10930153239080198 1103 50-51 -0.146102790571085 1103 52-53 -0.08137795268437742 1103 54-55 -0.12702587835369883 1103 56-57 -0.13923575571488556 1103 58-59 -0.10650729945234616 1103 60-61 -0.10314982731095768 1103 62-63 -0.1434274114587879 1103 64-65 -0.11404444068253383 1103 66-67 -0.12385687145427937 1103 68-69 -0.20655741630790914 1103 70-71 -0.22582865521870588 1103 72-73 -0.2458208185291184 1103 74-75 -0.2712612796748175 1103 76-77 -0.17665211863680952 1103 78-79 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0.01527866393458055 2204 36-37 0.008461316855182588 2204 38-39 0.027236358653205173 2204 40-41 0.05133436634679356 2204 42-43 -7.482842468249373E-4 2204 44-45 -0.002251872864718507 2204 46-47 0.015644533819838102 2204 48-49 -0.045638839331274994 2204 50-51 0.0375648538123059 2204 52-53 0.049037914115579895 2204 54-55 -0.015303909155221618 2204 56-57 0.021269437746276765 2204 58-59 -0.02558761833100931 2204 60-61 -0.07315726819129509 2204 62-63 -0.03538809503083229 2204 64-65 -0.07021688054183528 2204 66-67 0.04410528132850544 2204 68-69 0.05427641579617415 2204 70-71 0.022048639785651858 2204 72-73 0.026133194807805182 2204 74-75 -0.011614584980726761 2204 76-77 0.027992173305818824 2204 78-79 0.053276350420752294 2204 80-81 0.013487973064030712 2204 82-83 -0.048285111075770715 2204 84-85 -0.067604891036396 2204 86-87 0.00423754129130316 2204 88-89 -0.051423364053384546 2204 90-91 -0.09054267803369953 2204 92-93 -0.024475374651764525 2204 94-95 -0.11918835674916295 2204 96-97 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-0.14454796360040234 1215 3 -0.058155807468835974 1215 4 -0.06899292216333919 1215 5 -0.06026104680655919 1215 6 -0.04139615134722163 1215 7 -0.06410088846683948 1215 8 -0.07003157702164486 1215 9 -0.05074275093026159 1215 10-11 -0.06305227065735863 1215 12-13 -0.08841484127845689 1215 14-15 -0.07115725467050993 1215 16-17 -0.006028107334358879 1215 18-19 -0.011320523818902473 1215 20-21 -0.002222109754200119 1215 22-23 -0.034143594427881396 1215 24-25 0.001834178737105674 1215 26-27 0.012614695655457808 1215 28-29 0.03491675309684439 1215 30-31 -0.0369552310065302 1215 32-33 0.008107684018298755 1215 34-35 0.010326375690482337 1215 36-37 -0.007846375612565737 1215 38-39 0.05323837203572879 1215 40-41 0.04402186380163897 1215 42-43 0.07681127491350992 1215 44-45 0.025506572284399454 1215 46-47 0.0757588679976422 1215 48-49 0.12327183593584579 1215 50-51 0.13519435115182432 1215 52-53 0.12930839281818862 1215 54-55 0.13403294495948614 1215 56-57 0.11918843034757742 1215 58-59 0.15742092015273101 1215 60-61 0.16194183408711638 1215 62-63 0.17342485085381298 1215 64-65 0.23500828330651302 1215 66-67 0.3064860102987126 1215 68-69 0.280822814922395 1215 70-71 0.24089755888741848 1215 72-73 0.20564216771371235 1215 74-75 0.22765522648332137 1215 76-77 0.23172854020782552 1215 78-79 0.33542127977921865 1215 80-81 0.3181547565476137 1215 82-83 0.28191229946789065 1215 84-85 0.26399137412194307 1215 86-87 0.4382545117711061 1215 88-89 0.4045465949031595 1215 90-91 0.42301552747726845 1215 92-93 0.4307578593723278 1215 94-95 0.4021350465524449 1215 96-97 0.474970302336871 1215 98-99 0.5005491105311748 1215 100-101 0.6328881293001345 1212 1 -0.1325032599401581 1212 2 -0.1335240861174256 1212 3 -0.08756657533773904 1212 4 -0.06726394216656928 1212 5 -0.06020940945527542 1212 6 -0.06012539330625799 1212 7 -0.1565930993005722 1212 8 -0.21866339050916395 1212 9 -0.3001031215574983 1212 10-11 -0.2737653438522827 1212 12-13 -0.20237880955952647 1212 14-15 -0.18220240569669244 1212 16-17 -0.05653192727857004 1212 18-19 -0.04061423637602246 1212 20-21 -0.01895225580251747 1212 22-23 0.012806411148254426 1212 24-25 0.026505128573944603 1212 26-27 -0.004091860719867668 1212 28-29 0.040540134710226994 1212 30-31 0.07984383986965327 1212 32-33 -0.023631680081365403 1212 34-35 0.010977280767207276 1212 36-37 -0.11698824879959346 1212 38-39 -0.10705562687925152 1212 40-41 -0.1783765125550758 1212 42-43 -0.21234321863965988 1212 44-45 -0.0259237867026485 1212 46-47 -0.02698707449147264 1212 48-49 -0.1969640883843553 1212 50-51 -0.08065803979113895 1212 52-53 -0.12496586134444954 1212 54-55 -0.2580259898705748 1212 56-57 -0.14915917295907377 1212 58-59 -0.1773447978736442 1212 60-61 -0.1896819761917996 1212 62-63 -0.16245280550745633 1212 64-65 0.039819792453116065 1212 66-67 0.004700942205950298 1212 68-69 -0.08364227236157973 1212 70-71 0.004532266448080691 1212 72-73 -0.04847405233923752 1212 74-75 0.04996574964113165 1212 76-77 -0.04584255989429664 1212 78-79 0.17120465731505163 1212 80-81 0.1528359176851879 1212 82-83 0.14511022415751285 1212 84-85 0.19894999163496152 1212 86-87 0.22273659465572848 1212 88-89 0.15134328575631883 1212 90-91 0.25344959633790864 1212 92-93 0.29115776132525895 1212 94-95 0.17364037699083212 1212 96-97 0.33114729705464185 1212 98-99 0.28173587800974786 1212 100-101 0.33221634275233924 1213 1 -0.27315553739024523 1213 2 -0.22922010526529135 1213 3 -0.16183699898632398 1213 4 -0.1877503408713963 1213 5 -0.11110723738391926 1213 6 -0.03604183651451365 1213 7 -0.06025513954333661 1213 8 -0.07689476022819264 1213 9 -0.10078133320696736 1213 10-11 -0.11006257293551158 1213 12-13 -0.14563594003421798 1213 14-15 -0.19188528036568897 1213 16-17 -0.30736026005668293 1213 18-19 -0.34108625081400135 1213 20-21 -0.16586023280635231 1213 22-23 -0.24889540333283122 1213 24-25 -0.29051251720591864 1213 26-27 -0.1566214635077543 1213 28-29 -0.14630725991946036 1213 30-31 -0.15368450507588705 1213 32-33 -0.1578732134813805 1213 34-35 -0.04502175249191254 1213 36-37 -0.009610110669974858 1213 38-39 -0.017419415268484784 1213 40-41 0.003758523391191204 1213 42-43 0.028944828474386952 1213 44-45 -0.010168973578231544 1213 46-47 0.01713084809647114 1213 48-49 0.02502183382691925 1213 50-51 0.07914977319375538 1213 52-53 0.1018563537182331 1213 54-55 0.09951623820099798 1213 56-57 0.10311107160632815 1213 58-59 0.08041071527373589 1213 60-61 0.09129371316386425 1213 62-63 0.14040669537300943 1213 64-65 0.14510094514839267 1213 66-67 0.1762515449929083 1213 68-69 0.14169539012730326 1213 70-71 0.16098369776052834 1213 72-73 0.05394881256740547 1213 74-75 0.12930567188884368 1213 76-77 0.1974500951024254 1213 78-79 0.1912080385549615 1213 80-81 0.17928611521426774 1213 82-83 0.3043379966738229 1213 84-85 0.2227226926301782 1213 86-87 0.2569372480781169 1213 88-89 0.29437476086422265 1213 90-91 0.30266542578255695 1213 92-93 0.36567049093890347 1213 94-95 0.4264216740196929 1213 96-97 0.3467613468976225 1213 98-99 0.3900533021914043 1213 100-101 0.2824547599263454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 6.0 14 18.0 15 79.0 16 127.0 17 267.0 18 703.0 19 1624.0 20 3348.0 21 6464.0 22 11527.0 23 18564.0 24 26550.0 25 37970.0 26 51129.0 27 66656.0 28 84355.0 29 104722.0 30 127834.0 31 153687.0 32 184167.0 33 224369.0 34 277089.0 35 357789.0 36 497570.0 37 792225.0 38 1723375.0 39 5380748.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.753837989814093 32.88272496363058 33.75038513008185 17.61305191647347 2 16.523428799037255 34.102116528116596 32.803881382208985 16.570573290637157 3 16.53195778292665 32.810879977641285 33.25939641340804 17.39776582602402 4 16.916080411630873 32.42175387611243 33.290640979409574 17.371524732847117 5 17.45029735629128 32.613435242330915 32.993383375956604 16.942884025421197 6 17.299601044590908 32.989189143312686 32.146108906754016 17.565100905342387 7 17.28581435517078 33.000094148186875 32.82406467131723 16.890026825325112 8 16.840584224040313 33.076212069087006 32.88273458441866 17.200469122454027 9 16.854122641594156 32.41987506707075 33.31313850438722 17.412863786947877 10-11 17.006967268080686 32.77427123169327 32.90766275142103 17.311098748805012 12-13 16.71667178856488 32.7815696930473 33.22497903377118 17.27677948461664 14-15 16.803823966067526 32.548205558160674 33.21303460074655 17.434935875025246 16-17 16.87847541518462 32.52991080002076 33.216235292306436 17.375378492488178 18-19 16.871409366777453 32.376377213296564 33.20669217944368 17.545521240482298 20-21 16.986136020224716 32.2762159367426 33.04136831242317 17.69627973060952 22-23 16.976887467255324 32.30519679177147 33.11101887072503 17.606896870248175 24-25 17.09349653471097 32.19393006221997 32.97632538534545 17.736248017723614 26-27 17.17931356210123 32.23673153420628 32.8463111342439 17.737643769448592 28-29 17.17235521565503 32.23637251754049 32.86562305257443 17.72564921423005 30-31 17.310079602233095 32.06862426527136 32.8015974233579 17.81969870913764 32-33 17.382398031843195 32.17791397722542 32.743473838606484 17.6962141523249 34-35 17.331940766050177 32.151161155366985 32.75930150562085 17.75759657296199 36-37 17.350432065842817 32.07616628563916 32.72818323113164 17.845218417386384 38-39 17.413337361561926 32.07072186307086 32.74328535763746 17.772655417729755 40-41 17.477881390560754 32.13257051602192 32.56772281957492 17.8218252738424 42-43 17.36308463779794 32.051869191670995 32.69421090028612 17.890835270244942 44-45 17.40993332613136 31.99016175375356 32.6432647232554 17.95664019685968 46-47 17.454974500729872 32.15561624268394 32.57839398841117 17.81101526817502 48-49 17.396394465001176 31.926200673713335 32.66871556220951 18.00868929907597 50-51 17.482556825416253 31.990964785958997 32.49107977420848 18.03539861441627 52-53 17.471789899687 32.066960413818876 32.57780843278251 17.883441253711606 54-55 17.52059562921437 31.924534339571093 32.55401200029236 18.000858030922178 56-57 17.569385487068516 31.988372299035223 32.50222042737916 17.940021786517104 58-59 17.509542613375455 31.96505414961493 32.5914291843509 17.933974052658712 60-61 17.531836564235363 31.929894149280784 32.44130635034736 18.09696293613649 62-63 17.491629002771646 31.883931317161906 32.5478327190128 18.076606961053646 64-65 17.53430438228328 31.906062074570666 32.554655612537566 18.00497793060849 66-67 17.613949791138126 31.940838596720987 32.418359119849505 18.026852492291383 68-69 17.497264265984406 31.878476168519637 32.51024123805189 18.11401832744407 70-71 17.611211658811378 31.887994341409986 32.3789611104677 18.121832889310937 72-73 17.545801176355628 31.905912277706 32.48603090030983 18.062255645628543 74-75 17.641426577773483 31.85089636964778 32.43387851035733 18.073798542221407 76-77 17.586633608385732 31.97215384440725 32.41353915288731 18.027673394319706 78-79 17.60957050738117 31.872205680107307 32.46640399779988 18.051819814711642 80-81 17.69586537549453 31.904980656995875 32.327937678344 18.071216289165598 82-83 17.586250128333717 32.06730313608112 32.38871986132062 17.957726874264548 84-85 17.55811420039662 31.870232407452413 32.40122948524127 18.1704239069097 86-87 17.593522739066714 31.975242711475044 32.3634399250759 18.06779462438234 88-89 17.613808884521063 31.960791232087686 32.353490823414226 18.071909059977017 90-91 17.593770841357344 31.833333785679972 32.3443061936042 18.228589179358483 92-93 17.561640487922404 31.912721792892857 32.393283136391915 18.132354582792825 94-95 17.588321009674278 31.874232696338563 32.36324419973087 18.17420209425629 96-97 17.624419314682513 31.928955472592403 32.27838569062285 18.16823952210224 98-99 17.562043259636383 31.859450200175203 32.3967018169142 18.18180472327421 100-101 17.70412042864735 31.895220523833235 32.24756619711916 18.15309285040026 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 866.0 1 849.0 2 886.5 3 1028.5 4 1355.0 5 1799.5 6 2341.0 7 3195.5 8 4478.0 9 6252.5 10 8789.5 11 12463.0 12 17985.0 13 26199.0 14 38404.0 15 54608.0 16 75067.0 17 99992.0 18 128010.5 19 158696.5 20 189889.5 21 218557.0 22 244895.0 23 265360.0 24 281228.0 25 291460.5 26 293084.0 27 291909.0 28 288764.0 29 285724.0 30 287669.0 31 291837.0 32 296963.0 33 301235.0 34 303186.0 35 308389.0 36 324347.5 37 345111.5 38 357558.0 39 359615.5 40 353449.5 41 335709.5 42 305412.0 43 269529.0 44 235421.0 45 209189.0 46 191386.0 47 181995.0 48 173834.5 49 161924.0 50 149102.0 51 135858.0 52 121779.0 53 109536.5 54 99945.0 55 91551.5 56 84498.0 57 77502.5 58 69297.5 59 61038.0 60 52158.5 61 42386.0 62 33468.5 63 26404.5 64 21199.5 65 16758.5 66 12956.0 67 9868.5 68 7422.5 69 5449.0 70 3932.0 71 2802.0 72 2161.0 73 1749.0 74 1374.0 75 1098.5 76 895.5 77 746.0 78 645.5 79 507.5 80 408.0 81 329.5 82 233.0 83 166.5 84 114.5 85 67.5 86 34.5 87 18.5 88 9.5 89 8.0 90 5.5 91 2.0 92 1.0 93 2.0 94 2.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09630945028709276 2 0.0027928655017160826 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 4.9343913457881314E-5 10-11 8.881904422418638E-5 12-13 0.0 14-15 0.0012977449239422786 16-17 0.0 18-19 0.0 20-21 1.2829417499049143E-4 22-23 1.7763808844837277E-4 24-25 4.0462009035462684E-4 26-27 0.001569136447960626 28-29 0.0019096094508200072 30-31 0.003893234771826836 32-33 0.004302789253527251 34-35 0.005891663266871029 36-37 0.007006835711019147 38-39 0.011156658832826965 40-41 0.012059652449106194 42-43 0.012661648193292347 44-45 0.014620601557570233 46-47 0.013791623811477827 48-49 0.016283491441100837 50-51 0.014724223775831784 52-53 0.015523595173849462 54-55 0.016041706265157215 56-57 0.014472569817196591 58-59 0.01157114770587317 60-61 0.00618279235627253 62-63 0.0035330242035843023 64-65 3.602105682425336E-4 66-67 0.0030445194603512774 68-69 0.003927775511247353 70-71 0.003972185033359446 72-73 0.016500604660315513 74-75 0.011610622836639474 76-77 0.033326879149453044 78-79 0.025560147171182525 80-81 0.018839506158219086 82-83 0.030810339563101096 84-85 0.010653350915556576 86-87 0.01620947557091401 88-89 0.00779140393499946 90-91 0.006064366963973614 92-93 0.012523485235610277 94-95 0.012814614325011778 96-97 0.007431193366756927 98-99 0.008270039895540909 100-101 0.0015493988825774734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0132962E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.426007803572457 #Duplication Level Percentage of deduplicated Percentage of total 1 28.81796761648089 8.19179772349184 2 17.332725031688174 9.854003540158873 3 15.53977632652128 13.25201409370393 4 12.7902538031916 14.543034176767868 5 8.575604552540932 12.188510096544002 6 5.859969440185086 9.994532222123844 7 3.7372073935729175 7.436372057329072 8 2.347674042575725 5.338800052360164 9 1.4618412170937651 3.7398878860222107 >10 3.4943830366257167 14.557932326232393 >50 0.039909296372999475 0.7291377309445382 >100 0.002238565964575847 0.09488803276953517 >500 4.496771863465207E-4 0.07909006155174328 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.960634807472879E-5 0.0 0.0 2 2.960634807472879E-5 0.0 0.0 3 2.960634807472879E-5 0.0 0.0 4 2.960634807472879E-5 0.0 0.0 5 2.960634807472879E-5 0.0 0.0 6 2.960634807472879E-5 0.0 0.0 7 2.960634807472879E-5 0.0 0.0 8 2.960634807472879E-5 0.0 0.0 9 2.960634807472879E-5 0.0 0.0 10-11 2.960634807472879E-5 0.0 0.0 12-13 3.4540739420516926E-5 0.0 0.0 14-15 1.381629576820677E-4 0.0 0.0 16-17 1.87506871139949E-4 0.0 8.388465287839823E-5 18-19 2.0231004517731342E-4 0.0 1.5296613171943209E-4 20-21 2.713915240183472E-4 0.0 1.5790052306522022E-4 22-23 3.898169163172624E-4 0.0 1.9737565383152528E-4 24-25 5.674550047656352E-4 0.0 2.121788278688897E-4 26-27 7.746994412887367E-4 0.0 2.2698200190625407E-4 28-29 9.128623989708044E-4 0.0 2.2698200190625407E-4 30-31 0.001090500487419177 0.0 2.2698200190625407E-4 32-33 0.0012977449239422786 0.0 2.2698200190625407E-4 34-35 0.0015592676652690495 9.868782691576264E-6 2.3685078459783032E-4 36-37 0.0017369057537174224 9.868782691576264E-6 2.467195672894066E-4 38-39 0.001988559712352617 1.9737565383152528E-5 2.467195672894066E-4 40-41 0.002329032715211998 2.960634807472879E-5 2.467195672894066E-4 42-43 0.0026744401094171673 2.960634807472879E-5 2.467195672894066E-4 44-45 0.0030198475036223365 2.960634807472879E-5 2.467195672894066E-4 46-47 0.003187616809379133 2.960634807472879E-5 2.467195672894066E-4 48-49 0.003478745898780633 2.960634807472879E-5 2.467195672894066E-4 50-51 0.0037501374227989805 2.960634807472879E-5 2.467195672894066E-4 52-53 0.004031397729508904 9.868782691576264E-5 2.713915240183472E-4 54-55 0.004362001949676709 1.0362221826155078E-4 2.960634807472879E-4 56-57 0.004707409343881877 1.085566096073389E-4 2.960634807472879E-4 58-59 0.004978800867900225 1.085566096073389E-4 3.0593226343886417E-4 60-61 0.00528473313133909 1.085566096073389E-4 3.1580104613044044E-4 62-63 0.005610402960161106 1.1842539229891516E-4 3.503417855509574E-4 64-65 0.006064366963973614 1.1842539229891516E-4 3.7994813362568616E-4 66-67 0.006375233618758266 1.2829417499049143E-4 4.0462009035462684E-4 68-69 0.006740378578346588 1.2829417499049143E-4 4.1448887304620306E-4 70-71 0.007164736234084368 1.2829417499049143E-4 4.1448887304620306E-4 72-73 0.007589093889822146 1.2829417499049143E-4 4.1448887304620306E-4 74-75 0.00802825471959729 1.7763808844837274E-4 4.3916082977514374E-4 76-77 0.008556234593596621 1.7763808844837274E-4 4.6383278650408437E-4 78-79 0.009414818687763756 1.87506871139949E-4 4.6383278650408437E-4 80-81 0.01006615834540779 1.87506871139949E-4 4.6383278650408437E-4 82-83 0.010826054612659161 1.87506871139949E-4 4.7370156919566064E-4 84-85 0.011689573098172085 1.87506871139949E-4 4.7370156919566064E-4 86-87 0.01265177941060077 1.87506871139949E-4 4.7370156919566064E-4 88-89 0.0140531465528046 1.87506871139949E-4 4.7370156919566064E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 925 5.456968E-12 10.781653 5 GCCGTCT 985 1.8189894E-12 10.607042 6 ATGCCGT 995 2.419256E-10 9.545853 4 TATGCCG 865 2.226625E-8 9.333417 3 GGGCGAT 425 0.007450621 8.939719 9 CGTATGC 1260 1.5961086E-7 7.167119 1 TGTGCGA 870 0.009088353 6.004561 6 >>END_MODULE