FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n01 11-60308.34100000001be6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n01 11-60308.34100000001be6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10132962
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9255.456968E-1210.7816535
GCCGTCT9851.8189894E-1210.6070426
ATGCCGT9952.419256E-109.5458534
TATGCCG8652.226625E-89.3334173
GGGCGAT4250.0074506218.9397199
CGTATGC12601.5961086E-77.1671191
TGTGCGA8700.0090883536.0045616
Produced by FastQC (version 0.11.2)