##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n01 10-60155.34100000001bcc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9680571 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.773839786929926 38.0 38.0 38.0 32.0 38.0 2 36.76252774758844 38.0 38.0 38.0 32.0 38.0 3 36.74904837741493 38.0 38.0 38.0 32.0 38.0 4 36.747648046793934 38.0 38.0 38.0 32.0 38.0 5 36.737115197027116 38.0 38.0 38.0 32.0 38.0 6 38.689075468792076 40.0 38.0 40.0 38.0 40.0 7 38.63689393941742 40.0 38.0 40.0 38.0 40.0 8 38.63097176809095 40.0 38.0 40.0 38.0 40.0 9 38.632369412919964 40.0 38.0 40.0 38.0 40.0 10-11 38.64761340007733 40.0 38.0 40.0 38.0 40.0 12-13 38.638893769799324 40.0 38.0 40.0 38.0 40.0 14-15 38.62509701132299 40.0 38.0 40.0 38.0 40.0 16-17 38.607920648482406 40.0 38.0 40.0 38.0 40.0 18-19 38.5790091824129 40.0 38.0 40.0 38.0 40.0 20-21 38.55605268532197 40.0 38.0 40.0 38.0 40.0 22-23 38.5382896835321 40.0 38.0 40.0 38.0 40.0 24-25 38.49221962216898 40.0 38.0 40.0 38.0 40.0 26-27 38.413238898821156 40.0 38.0 40.0 38.0 40.0 28-29 38.375275900564134 40.0 38.0 40.0 38.0 40.0 30-31 38.324994000870404 40.0 38.0 40.0 38.0 40.0 32-33 38.288089359604925 40.0 38.0 40.0 38.0 40.0 34-35 38.25770706087482 40.0 38.0 40.0 38.0 40.0 36-37 38.21437655898603 40.0 38.0 40.0 38.0 40.0 38-39 38.1632717222982 40.0 38.0 40.0 38.0 40.0 40-41 38.116130598081455 40.0 38.0 40.0 38.0 40.0 42-43 38.076735917747 40.0 38.0 40.0 38.0 40.0 44-45 38.053291639511755 40.0 38.0 40.0 38.0 40.0 46-47 38.01626314191591 40.0 38.0 40.0 38.0 40.0 48-49 37.97045670136607 40.0 38.0 40.0 38.0 40.0 50-51 37.916091313208696 40.0 38.0 40.0 38.0 40.0 52-53 37.86992151599322 40.0 38.0 40.0 32.0 40.0 54-55 37.81773213584199 40.0 38.0 40.0 32.0 40.0 56-57 37.76339045496387 40.0 38.0 40.0 32.0 40.0 58-59 37.73537800611142 40.0 38.0 40.0 32.0 40.0 60-61 37.6733330089723 40.0 38.0 40.0 32.0 40.0 62-63 37.63334776430027 40.0 38.0 40.0 32.0 40.0 64-65 37.59066174918814 40.0 38.0 40.0 32.0 40.0 66-67 37.4992425033606 40.0 38.0 40.0 32.0 40.0 68-69 37.44016029632963 40.0 38.0 40.0 32.0 40.0 70-71 37.417665548860704 40.0 38.0 40.0 32.0 40.0 72-73 37.362867076745786 40.0 38.0 40.0 32.0 40.0 74-75 37.28731037662964 39.0 38.0 40.0 32.0 40.0 76-77 37.213559613374045 38.0 38.0 40.0 32.0 40.0 78-79 37.12955707881281 38.0 38.0 40.0 32.0 40.0 80-81 37.082882042805124 38.0 38.0 40.0 32.0 40.0 82-83 37.00078910634507 38.0 38.0 40.0 32.0 40.0 84-85 36.92920655196889 38.0 38.0 40.0 32.0 40.0 86-87 36.86708986484372 38.0 38.0 40.0 32.0 40.0 88-89 36.81915281650225 38.0 38.0 40.0 32.0 40.0 90-91 36.750709591407364 38.0 38.0 40.0 32.0 40.0 92-93 36.67745353037543 38.0 38.0 40.0 32.0 40.0 94-95 36.59144006071543 38.0 38.0 40.0 32.0 40.0 96-97 36.500522076641964 38.0 38.0 40.0 32.0 40.0 98-99 36.3944634567527 38.0 38.0 40.0 32.0 40.0 100-101 35.50753927635054 38.0 35.0 39.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.0798871095987721 2201 2 0.1270027352110148 2201 3 0.06633166200624885 2201 4 0.04502135410915997 2201 5 0.13148396957912212 2201 6 0.19824959665685782 2201 7 0.19913209180203495 2201 8 0.1471450483377481 2201 9 0.10090072378672232 2201 10-11 0.08968981224029449 2201 12-13 0.02711248877800898 2201 14-15 0.06437990013822059 2201 16-17 0.06198773841067151 2201 18-19 0.050500789289628756 2201 20-21 0.1272805703030926 2201 22-23 0.11077054951312704 2201 24-25 0.09484617971861553 2201 26-27 0.09510283420456034 2201 28-29 0.06482654220219786 2201 30-31 0.047432630186186486 2201 32-33 0.034934288912914724 2201 34-35 0.03646878533056963 2201 36-37 0.066661856850736 2201 38-39 0.0812096311271091 2201 40-41 0.042159187449215096 2201 42-43 -0.029441634861186117 2201 44-45 -0.040517708342356684 2201 46-47 -0.06089916629194647 2201 48-49 -0.010827411362868133 2201 50-51 0.002552402775812368 2201 52-53 -0.014681424975556467 2201 54-55 -0.15922446859849515 2201 56-57 -0.09074556554404722 2201 58-59 -0.012175473657265456 2201 60-61 -0.01237171154611616 2201 62-63 -0.06952861282663747 2201 64-65 -0.08733131211537426 2201 66-67 -0.04907291261933722 2201 68-69 -0.09380793329670212 2201 70-71 -0.06211527941938755 2201 72-73 -0.07906891631063218 2201 74-75 -0.006534034501598285 2201 76-77 -0.05357291212934001 2201 78-79 -0.023447370654494648 2201 80-81 -0.09658336542909751 2201 82-83 -0.1865868426725541 2201 84-85 -0.11694234861975161 2201 86-87 -0.255449023508703 2201 88-89 -0.21901800673324345 2201 90-91 -0.21702394907180889 2201 92-93 -0.1822295389563635 2201 94-95 -0.21813367406449657 2201 96-97 -0.1771232234877118 2201 98-99 -0.27305508583276605 2201 100-101 -0.38973375091401863 1101 1 0.12828192393058657 1101 2 0.0991042070165804 1101 3 0.06021800478531958 1101 4 -0.0047386177675718955 1101 5 -0.006740019730877123 1101 6 0.025638818704685207 1101 7 0.11354280025297214 1101 8 0.04377256288746878 1101 9 0.055316573222519594 1101 10-11 0.06368994966585717 1101 12-13 0.046013237063576184 1101 14-15 0.040892732411556665 1101 16-17 -0.02292531897940364 1101 18-19 0.031981891257828465 1101 20-21 -0.014511284673083935 1101 22-23 0.005105016037546761 1101 24-25 -0.009661554195801614 1101 26-27 -0.0821516002259699 1101 28-29 -0.007425774328602586 1101 30-31 0.03191389659074417 1101 32-33 -0.042973301915083084 1101 34-35 0.0022526452314082235 1101 36-37 -0.04601242021058738 1101 38-39 -0.05901066309233016 1101 40-41 -0.0564437009453016 1101 42-43 -0.03445057784026062 1101 44-45 -0.07754546082124847 1101 46-47 -0.15882736048221346 1101 48-49 -0.16480179581756005 1101 50-51 -0.12850934642118972 1101 52-53 -0.07713437820370928 1101 54-55 -0.15664616551957522 1101 56-57 -0.09958198591682077 1101 58-59 -0.10250869121679784 1101 60-61 -0.12369832186830365 1101 62-63 -0.10504920853532695 1101 64-65 -0.1334199025692513 1101 66-67 -0.11790068707030343 1101 68-69 -0.10432894402319448 1101 70-71 -0.17647409447597084 1101 72-73 -0.1455064900725418 1101 74-75 -0.19203640792602528 1101 76-77 -0.19354100180348865 1101 78-79 -0.18092512131161698 1101 80-81 -0.23328770585617065 1101 82-83 -0.3513552595070095 1101 84-85 -0.25987560571916646 1101 86-87 -0.2913005400884572 1101 88-89 -0.35069706526159194 1101 90-91 -0.3443694210058723 1101 92-93 -0.30080071845111434 1101 94-95 -0.3771403934105493 1101 96-97 -0.353270113239617 1101 98-99 -0.40010281676029535 1101 100-101 -0.37978455309645653 2202 1 0.051929534873060845 2202 2 0.0393457256980696 2202 3 0.032180681554415 2202 4 0.040636515925498884 2202 5 0.033043767467148655 2202 6 0.022375418665511404 2202 7 0.049122244650845914 2202 8 0.04457012111299008 2202 9 0.023422223247848706 2202 10-11 0.08008058284134023 2202 12-13 0.056624935531424114 2202 14-15 0.04586845308769938 2202 16-17 0.05511031437789882 2202 18-19 -0.0058750697543601405 2202 20-21 0.021493378983869604 2202 22-23 -0.043791477989252314 2202 24-25 0.0193502876942091 2202 26-27 0.015821167205700704 2202 28-29 -0.00975534227477226 2202 30-31 0.010062860474270963 2202 32-33 -0.026303641015765322 2202 34-35 -0.11215309329385548 2202 36-37 -0.017616039900126168 2202 38-39 0.004352191099457059 2202 40-41 -0.03230522576886585 2202 42-43 -0.08677658358425333 2202 44-45 -0.09636990913724475 2202 46-47 -0.1073508424598657 2202 48-49 -0.15019753730862107 2202 50-51 -0.16003380549157242 2202 52-53 -0.17205431835919427 2202 54-55 -0.13109613376136053 2202 56-57 -0.2414331708640134 2202 58-59 -0.1518974284908694 2202 60-61 -0.11054975553359014 2202 62-63 -0.2449711246096271 2202 64-65 -0.2721456067244077 2202 66-67 -0.09235272771405789 2202 68-69 -0.14525784977183775 2202 70-71 -0.19078342091971479 2202 72-73 -0.17091491764984568 2202 74-75 -0.22235084404105976 2202 76-77 -0.28502417384869716 2202 78-79 -0.27158693535648837 2202 80-81 -0.2583889939053563 2202 82-83 -0.24290360053161208 2202 84-85 -0.3157882076664933 2202 86-87 -0.3389428128579013 2202 88-89 -0.2706480963738471 2202 90-91 -0.44548881429927434 2202 92-93 -0.3989766141239812 2202 94-95 -0.373758490201503 2202 96-97 -0.4393218332618858 2202 98-99 -0.48584565333116814 2202 100-101 -0.4454542146467517 1102 1 0.10944024520110673 1102 2 0.1023518037317217 1102 3 0.07759398990982902 1102 4 -0.025554743106965816 1102 5 0.007320963610560227 1102 6 0.02797094024693081 1102 7 0.0017386796534637483 1102 8 0.009582857655836108 1102 9 0.01156288859421295 1102 10-11 -0.02776108510316533 1102 12-13 -0.028505853039611395 1102 14-15 0.020478033254867967 1102 16-17 -0.017084340032447187 1102 18-19 -0.06070109833201798 1102 20-21 -0.07121142679547887 1102 22-23 -0.07155461096645155 1102 24-25 -0.0817297193207338 1102 26-27 -0.1078554568526755 1102 28-29 -0.14594551925780053 1102 30-31 -0.13916827898004414 1102 32-33 -0.12145359899753316 1102 34-35 -0.10203261607963299 1102 36-37 -0.08687723690965043 1102 38-39 -0.10092214043757508 1102 40-41 -0.23092032698376386 1102 42-43 -0.2190859990093088 1102 44-45 -0.25858811706136464 1102 46-47 -0.2516590621691961 1102 48-49 -0.2524998561665228 1102 50-51 -0.2690154747073379 1102 52-53 -0.21926744265932285 1102 54-55 -0.19145173387046555 1102 56-57 -0.28951892581294913 1102 58-59 -0.2724524051055397 1102 60-61 -0.30983665993778686 1102 62-63 -0.28582375255337666 1102 64-65 -0.38587579698658914 1102 66-67 -0.277354306921346 1102 68-69 -0.35625856474895556 1102 70-71 -0.40395057198935547 1102 72-73 -0.38125276136644004 1102 74-75 -0.38067696840940357 1102 76-77 -0.366085077106419 1102 78-79 -0.46085905116446213 1102 80-81 -0.43455385659254375 1102 82-83 -0.5427914352375893 1102 84-85 -0.42983581612700306 1102 86-87 -0.42722325437446784 1102 88-89 -0.44517224713474945 1102 90-91 -0.4722437932535044 1102 92-93 -0.5297884486982269 1102 94-95 -0.6206628839399997 1102 96-97 -0.7097231513397446 1102 98-99 -0.82643217674309 1102 100-101 -0.8329145165983292 2203 1 0.04163781339870809 2203 2 0.013699384710690765 2203 3 -0.038615880539246916 2203 4 -0.015489632957638833 2203 5 0.028479684709701303 2203 6 -0.1117320646235811 2203 7 -0.019990921942444118 2203 8 -0.10011083297553114 2203 9 -0.05285344448973461 2203 10-11 -0.06349479652445922 2203 12-13 -0.05936185264368987 2203 14-15 -0.061825892553692086 2203 16-17 -0.09455905229554418 2203 18-19 -0.13170942027046806 2203 20-21 -0.09352626726893476 2203 22-23 -0.03428752548038716 2203 24-25 -0.10357650642241367 2203 26-27 -0.1620415411920817 2203 28-29 -0.09954656098530279 2203 30-31 -0.09391426097484157 2203 32-33 -0.09089511973439102 2203 34-35 -0.15409565141423798 2203 36-37 -0.208013839674976 2203 38-39 -0.17549279845404442 2203 40-41 -0.14214786455383432 2203 42-43 -0.053517174595938855 2203 44-45 -0.14310024017165546 2203 46-47 -0.17898953525798333 2203 48-49 -0.2282089880140603 2203 50-51 -0.22234803996485653 2203 52-53 -0.18983502313879086 2203 54-55 -0.3324129105075144 2203 56-57 -0.25336031650488167 2203 58-59 -0.2169305552080374 2203 60-61 -0.21643517491163777 2203 62-63 -0.26650913648843755 2203 64-65 -0.316305470900069 2203 66-67 -0.3100686781202171 2203 68-69 -0.3366635757618681 2203 70-71 -0.38536016464566103 2203 72-73 -0.4141655674536011 2203 74-75 -0.4172874420436017 2203 76-77 -0.3793305341652342 2203 78-79 -0.3961956529289452 2203 80-81 -0.49539627851692103 2203 82-83 -0.48858229255526453 2203 84-85 -0.5306440621209774 2203 86-87 -0.49130617690431677 2203 88-89 -0.6137818173501941 2203 90-91 -0.5751797136938919 2203 92-93 -0.691970887455085 2203 94-95 -0.6739237938761562 2203 96-97 -0.7625747441260557 2203 98-99 -0.640599964075065 2203 100-101 -0.6421711466122204 1103 1 0.06616390787014126 1103 2 0.01968374304961884 1103 3 0.05635468046489933 1103 4 0.06658988321955661 1103 5 0.030279427464989794 1103 6 0.025542334749360407 1103 7 0.002822550826813597 1103 8 -0.02308206085173481 1103 9 -0.027471781676197793 1103 10-11 -0.0038903835263184305 1103 12-13 -0.01487799142276458 1103 14-15 -0.019354131655042295 1103 16-17 0.011801777894170584 1103 18-19 -0.028523574959976372 1103 20-21 -0.003729558306311276 1103 22-23 0.003304317929810452 1103 24-25 0.05354613137602371 1103 26-27 0.03585386496759213 1103 28-29 -0.004437084801949709 1103 30-31 -1.6597593130995847E-6 1103 32-33 -0.05861436052456526 1103 34-35 -0.07313676278319292 1103 36-37 -0.07215312789070083 1103 38-39 -0.012868383650115334 1103 40-41 -0.03306842850160763 1103 42-43 -0.06650703911742539 1103 44-45 -0.10172427401898432 1103 46-47 -0.13297333351413698 1103 48-49 -0.11795265873554683 1103 50-51 -0.13975738060896248 1103 52-53 -0.061957965860926834 1103 54-55 -0.09501094479254846 1103 56-57 -0.08474585079321884 1103 58-59 -0.05671276049532992 1103 60-61 -0.08824684137105976 1103 62-63 -0.12731586292014896 1103 64-65 -0.21569020816276918 1103 66-67 -0.17800693925792643 1103 68-69 -0.19116687949780697 1103 70-71 -0.18763598105572044 1103 72-73 -0.18994142003210612 1103 74-75 -0.21752792585057534 1103 76-77 -0.17811972642898866 1103 78-79 -0.2643081561728948 1103 80-81 -0.1866812922701655 1103 82-83 -0.30117647569375094 1103 84-85 -0.2548122408659239 1103 86-87 -0.21495687639396976 1103 88-89 -0.3557441246905597 1103 90-91 -0.31312143393340364 1103 92-93 -0.31632193461054214 1103 94-95 -0.46078235192240413 1103 96-97 -0.38829011027339533 1103 98-99 -0.25915369915261266 1103 100-101 -0.30328168461640104 2204 1 0.10144140134266877 2204 2 0.009677170440909322 2204 3 -0.0016694239569758906 2204 4 0.05052337492809755 2204 5 0.07068026299241126 2204 6 0.030751295264771272 2204 7 0.08038749539445433 2204 8 0.06767172758224405 2204 9 0.07784206910665858 2204 10-11 0.06752948685124949 2204 12-13 0.12481911183090233 2204 14-15 0.057691469833919484 2204 16-17 0.058625754329909796 2204 18-19 0.06616683105282561 2204 20-21 0.06734068303511265 2204 22-23 0.04729107313118419 2204 24-25 0.06618376327183029 2204 26-27 0.039426574254264324 2204 28-29 0.03529048536138646 2204 30-31 0.03057113018024893 2204 32-33 0.00686780138467924 2204 34-35 0.08275921466321279 2204 36-37 0.05740728095844361 2204 38-39 0.04532283787838054 2204 40-41 0.05007867002206012 2204 42-43 -0.007255191084908574 2204 44-45 0.08027498246348586 2204 46-47 0.04703800095663979 2204 48-49 -0.022171879498287694 2204 50-51 -0.013676619420017744 2204 52-53 0.06505988447511868 2204 54-55 0.05037340400550505 2204 56-57 0.044312687867723355 2204 58-59 0.04223873739655204 2204 60-61 0.011378687215660932 2204 62-63 0.01164226548614522 2204 64-65 0.04160831028969625 2204 66-67 0.04659962728625544 2204 68-69 0.06072498442288321 2204 70-71 0.036951527455272526 2204 72-73 -0.022382958827833477 2204 74-75 0.03745439039086307 2204 76-77 0.022418754588585443 2204 78-79 0.10522675694011951 2204 80-81 0.07208177350851486 2204 82-83 -0.01645312579772451 2204 84-85 0.05045474261758187 2204 86-87 0.055888710014599496 2204 88-89 0.01271490978433576 2204 90-91 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0.43510325804450645 1214 96-97 0.35628295193666304 1214 98-99 0.32694276350216 1214 100-101 0.5033282970591344 1215 1 -0.18845102350729093 1215 2 -0.20368602644762746 1215 3 -0.07973864149060006 1215 4 -0.08029489249148014 1215 5 -0.05430551857494237 1215 6 -0.0788111095855939 1215 7 -0.10439589007108907 1215 8 -0.11026447193700051 1215 9 -0.10523329411562798 1215 10-11 -0.04730795855738279 1215 12-13 -0.14230867466856978 1215 14-15 -0.07382661151525838 1215 16-17 -0.03576455895071007 1215 18-19 -0.05846006564267725 1215 20-21 -0.04960835218802373 1215 22-23 -0.07832432103047182 1215 24-25 -0.07529934847403297 1215 26-27 -0.016355128263285224 1215 28-29 -0.02883591349441872 1215 30-31 -0.0677621901831813 1215 32-33 -0.068037629552812 1215 34-35 -0.04365430191990072 1215 36-37 -0.019225493972555796 1215 38-39 -0.033134713045228636 1215 40-41 -0.0010905221588046743 1215 42-43 0.019126589497830082 1215 44-45 -0.014998737212017943 1215 46-47 6.13689996669109E-4 1215 48-49 0.055875534254212766 1215 50-51 0.06962305222285892 1215 52-53 0.08072819381480656 1215 54-55 0.09363524515634936 1215 56-57 0.026370641650345306 1215 58-59 0.049600854642612546 1215 60-61 0.056269414372884796 1215 62-63 0.098698220537635 1215 64-65 0.13208145459276466 1215 66-67 0.1964051974386507 1215 68-69 0.19851454957188963 1215 70-71 0.15340924753380136 1215 72-73 0.11659699201484841 1215 74-75 0.12298129155074378 1215 76-77 0.1773677173167556 1215 78-79 0.22605121919747972 1215 80-81 0.25363712627841295 1215 82-83 0.2624786793800524 1215 84-85 0.24821470360956255 1215 86-87 0.3557806003678152 1215 88-89 0.3402369173295412 1215 90-91 0.2960313067366016 1215 92-93 0.3466006453136714 1215 94-95 0.34954388888813526 1215 96-97 0.4316898007356684 1215 98-99 0.4178098240483479 1215 100-101 0.5056767270242091 1212 1 -0.17693015010535618 1212 2 -0.1563286319449233 1212 3 -0.07737610261335703 1212 4 -0.11386738171773203 1212 5 -0.07398243876794908 1212 6 -0.1397716240165039 1212 7 -0.15584684250827507 1212 8 -0.2515087628262691 1212 9 -0.3600841007110276 1212 10-11 -0.3763546675153364 1212 12-13 -0.24300999768065878 1212 14-15 -0.2524452103675685 1212 16-17 -0.15250621026059008 1212 18-19 -0.12129053650097887 1212 20-21 -0.10484931665437358 1212 22-23 -0.07841516299691875 1212 24-25 -0.05495024745552257 1212 26-27 -0.08840961643154799 1212 28-29 -0.04764463501675209 1212 30-31 -0.04153191260287059 1212 32-33 -0.18210693286546586 1212 34-35 -0.1123391985924016 1212 36-37 -0.17838221075911065 1212 38-39 -0.2199902014303987 1212 40-41 -0.25831737222800655 1212 42-43 -0.33366507363838593 1212 44-45 -0.14753535550793373 1212 46-47 -0.12578760327782845 1212 48-49 -0.34426752842995256 1212 50-51 -0.20591504281115647 1212 52-53 -0.24950001521985854 1212 54-55 -0.36831511727260846 1212 56-57 -0.2916027381333919 1212 58-59 -0.27767022232606564 1212 60-61 -0.3428137681187735 1212 62-63 -0.316573665790834 1212 64-65 -0.12578125816269647 1212 66-67 -0.15545687640022265 1212 68-69 -0.2558773968075343 1212 70-71 -0.14199758334501666 1212 72-73 -0.2480814804870377 1212 74-75 -0.16451761987016766 1212 76-77 -0.2919859774891762 1212 78-79 -0.02283377974092815 1212 80-81 0.03171460890122546 1212 82-83 -0.031064144225645407 1212 84-85 0.09615754102082263 1212 86-87 0.05878410214111085 1212 88-89 0.01828220221993604 1212 90-91 0.16038377310842122 1212 92-93 0.16811453087203176 1212 94-95 0.09395497005082376 1212 96-97 0.14745573022192104 1212 98-99 0.14056870876757444 1212 100-101 0.13100212793374055 1213 1 -0.2875216672451373 1213 2 -0.19344011192429633 1213 3 -0.1357695218830841 1213 4 -0.13561388851454836 1213 5 -0.08950865243117079 1213 6 -0.07673170988757505 1213 7 -0.15906711125222728 1213 8 -0.150332957732644 1213 9 -0.10210252244508666 1213 10-11 -0.15255697475300423 1213 12-13 -0.20020385152925257 1213 14-15 -0.2875641157288982 1213 16-17 -0.3875313838498755 1213 18-19 -0.37124582646126214 1213 20-21 -0.21855234871689078 1213 22-23 -0.3132530790573895 1213 24-25 -0.3711040604636082 1213 26-27 -0.22548676896232678 1213 28-29 -0.22995456514316004 1213 30-31 -0.2144434195098981 1213 32-33 -0.23062509344315174 1213 34-35 -0.14039651420128507 1213 36-37 -0.11677807533270368 1213 38-39 -0.08196854807237486 1213 40-41 -0.07097353946809903 1213 42-43 -0.052279562115899125 1213 44-45 -0.08233475554877145 1213 46-47 0.002561661583918351 1213 48-49 0.018682174167338417 1213 50-51 0.03129291601384665 1213 52-53 0.061334940364453416 1213 54-55 0.024945534397794233 1213 56-57 0.06766608395068374 1213 58-59 0.08477736983208928 1213 60-61 0.06678507322691019 1213 62-63 0.06267665916856657 1213 64-65 0.05976760550927196 1213 66-67 0.1245363908619197 1213 68-69 0.07795806474238276 1213 70-71 0.0730040629944142 1213 72-73 -0.0024812712852195773 1213 74-75 0.07171637885667081 1213 76-77 0.12406284269559364 1213 78-79 0.13170447526862006 1213 80-81 0.10591506412855267 1213 82-83 0.18100246701214928 1213 84-85 0.10124039971166354 1213 86-87 0.10962581338095845 1213 88-89 0.0999808807407021 1213 90-91 0.21414769835097758 1213 92-93 0.22640843500876429 1213 94-95 0.27646388472974337 1213 96-97 0.23876603339751057 1213 98-99 0.2703710381110511 1213 100-101 0.14121128071177935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 24.0 15 51.0 16 106.0 17 210.0 18 560.0 19 1375.0 20 3343.0 21 6969.0 22 12835.0 23 20158.0 24 29055.0 25 39917.0 26 52800.0 27 67973.0 28 85698.0 29 105456.0 30 128257.0 31 153488.0 32 184800.0 33 223751.0 34 276881.0 35 355863.0 36 494867.0 37 788644.0 38 1710029.0 39 4937456.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.67370193385407 29.330347504783145 29.554253626958648 21.44169693440414 2 20.47639530999072 30.140175089217074 29.194586708995836 20.18884289179637 3 20.504410328688255 28.720712858776615 29.572336177277148 21.202540635257982 4 20.732909246778934 28.385629318766426 29.51350700284105 21.36795443161359 5 21.39478136155398 28.620925356572457 29.191604503494677 20.792688778378878 6 21.248064809400187 28.90687956319932 28.36806837117356 21.47698725622693 7 21.215556396415046 29.212595000852737 28.989302387224885 20.58254621550733 8 20.502860833312415 29.028742209524623 29.4308569194937 21.037540037669267 9 20.61149110496225 28.560644078042547 29.615437775022656 21.212427041972546 10-11 20.809544690831576 28.75934927351864 29.147245021339145 21.28386101431063 12-13 20.467082985084247 28.894881304005725 29.57190231857191 21.06613339233812 14-15 20.592314016321264 28.652350959891855 29.540704221125498 21.21463080266139 16-17 20.667463727088 28.688591819635434 29.411488227295685 21.232456225980883 18-19 20.527471984865358 28.47904322999129 29.602711451628217 21.39077333351514 20-21 20.747237492941398 28.32845939185255 29.40351044647349 21.520792668732557 22-23 20.754446062750816 28.361031198661607 29.383627051721543 21.500895686866034 24-25 20.850951584410744 28.299111237504444 29.32590840241437 21.524028775670445 26-27 20.986187124335302 28.306847494136235 29.10342929692804 21.60353608460042 28-29 20.924430127291934 28.398094270109386 29.150652109877235 21.52682349272144 30-31 21.161624454638375 28.147464925552107 29.04812673799435 21.64278388181517 32-33 21.123732796122447 28.331364435869943 29.054692133771255 21.490210634236355 34-35 21.186300908098183 28.187851690020683 29.028253280709816 21.59759412117132 36-37 21.22328733930461 28.24817880578338 28.91827744942364 21.610256405488375 38-39 21.17673379023665 28.138549181039618 29.02574958779883 21.658967440924894 40-41 21.329496367498663 28.204741444660097 28.809234546185152 21.656527641656083 42-43 21.218791344733486 28.201222693492063 28.909095154332665 21.67089080744179 44-45 21.177552120403192 27.9760708637671 28.898369359316106 21.9480076565136 46-47 21.286158501669437 28.273150416525795 28.711447182080907 21.729243899723865 48-49 21.19915302634387 27.965834865139815 28.898520461685262 21.93649164683106 50-51 21.277772663653778 28.07160744769362 28.71428752968904 21.93633235896357 52-53 21.300094756743583 28.14122376038131 28.802790413818297 21.755891069056812 54-55 21.345380049996226 27.992602075198285 28.703961661879895 21.95805621292559 56-57 21.402681723974712 28.109951643795327 28.696534531404982 21.79083210082498 58-59 21.271916038292613 28.09384867108412 28.807621330323506 21.82661396029976 60-61 21.337989290829437 28.054774787581067 28.573079262573025 22.03415665901647 62-63 21.311465248816514 28.029191145984605 28.754667196545775 21.90467640865311 64-65 21.369632131096804 27.947813040614157 28.62642180845058 22.05613301983846 66-67 21.426866933476965 28.024017059000116 28.521677975216846 22.027438032306073 68-69 21.170323592155096 27.973301569508752 28.725249270559594 22.131125567776557 70-71 21.499424933481382 27.96879542946728 28.417934596184608 22.11384504086673 72-73 21.470817021727672 27.981127492484458 28.542686513315964 22.005368972471903 74-75 21.423705458162107 27.892672189491424 28.60124426272645 22.08237808962002 76-77 21.44602320548989 28.048070123843694 28.50628776952695 21.999618901139463 78-79 21.42943420229285 27.939495900429918 28.6069521169884 22.024117780288837 80-81 21.558212832948133 27.938556026641375 28.414797498445942 22.088433641964546 82-83 21.408392984074638 28.187730345479856 28.60089652550642 21.80298014493908 84-85 21.371121229745146 27.92894344817241 28.537254733624458 22.162680588457988 86-87 21.340116305949955 28.04783180729536 28.49488025181835 22.117171634936337 88-89 21.40269135364495 27.962012282675296 28.471216334447785 22.164080029231968 90-91 21.429881564792463 27.93521520645854 28.383034598539247 22.251868630209746 92-93 21.273582525077785 27.965882155005815 28.60060560988006 22.159929710036337 94-95 21.38823120415668 27.79969352336297 28.48279084351328 22.329284428967078 96-97 21.46437043012802 28.023575598726385 28.294985071549227 22.217068899596367 98-99 21.382314931452342 28.00891464837827 28.432776777326513 22.175993642842876 100-101 21.64175195990838 27.887545663016724 28.1873000023656 22.283402374709297 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 159.0 1 134.0 2 105.0 3 127.5 4 128.5 5 131.5 6 168.0 7 210.5 8 227.0 9 259.0 10 371.5 11 465.0 12 610.5 13 875.5 14 1242.0 15 1726.5 16 2404.0 17 3452.5 18 5077.0 19 6965.5 20 9898.0 21 13738.5 22 18022.5 23 23271.0 24 29694.0 25 36976.5 26 44583.0 27 53700.5 28 65462.0 29 80151.5 30 97637.0 31 119092.0 32 148955.5 33 189748.0 34 239760.0 35 304027.0 36 385988.5 37 466564.5 38 523030.5 39 553435.0 40 563090.5 41 544849.5 42 499967.5 43 445849.0 44 393737.5 45 350865.0 46 317159.5 47 295363.0 48 282654.5 49 271357.5 50 261050.5 51 246105.0 52 224819.5 53 203240.0 54 185563.0 55 170581.5 56 156690.0 57 142971.5 58 127260.5 59 112496.5 60 96100.5 61 77803.0 62 62168.0 63 48522.0 64 38523.5 65 30410.0 66 23406.0 67 17886.5 68 13425.5 69 10024.0 70 7609.0 71 5881.5 72 4460.5 73 3532.5 74 2951.5 75 2415.5 76 2042.5 77 1755.0 78 1562.5 79 1423.0 80 1251.0 81 937.5 82 709.0 83 630.5 84 486.5 85 243.0 86 112.5 87 67.5 88 25.5 89 13.0 90 8.0 91 7.0 92 4.5 93 1.5 94 1.0 95 1.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09407502925189021 2 0.002572162323895977 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 5.164984586136499E-5 10-11 6.197981503363799E-5 12-13 0.0 14-15 0.0013583909461538993 16-17 0.0 18-19 0.0 20-21 7.74747687920475E-5 22-23 1.1879464548113949E-4 24-25 3.30559013512736E-4 26-27 0.0015133404837379943 28-29 0.0019162092814566413 30-31 0.004250782314390339 32-33 0.004643321142936713 34-35 0.006358096025534031 36-37 0.007515052572828607 38-39 0.011719350025943718 40-41 0.012618057343931469 42-43 0.013274010386370803 44-45 0.015412314005031316 46-47 0.01444646188742379 48-49 0.01693598445794158 50-51 0.015432973943375861 52-53 0.016285196400088384 54-55 0.016946314427113855 56-57 0.015097249945276989 58-59 0.01196726928607827 60-61 0.006642170177771539 62-63 0.003878903424188511 64-65 3.0989907516819E-4 66-67 0.002944041214097805 68-69 0.0038892333933607842 70-71 0.003992533085083514 72-73 0.01677586993577135 74-75 0.01140428596618939 76-77 0.03400625851512271 78-79 0.02564931345475386 80-81 0.019291217429219826 82-83 0.030865947886751722 84-85 0.011047902029745973 86-87 0.015810017818163824 88-89 0.007830116632582934 90-91 0.005707307967680832 92-93 0.012783336850687835 94-95 0.012432117898830553 96-97 0.0075615374341038355 98-99 0.007979901185580892 100-101 0.0014875155608073118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9680571.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.088166889729559 #Duplication Level Percentage of deduplicated Percentage of total 1 34.89031084997996 4.915405220890596 2 2.6443059130033495 0.7450684601977976 3 3.321455159268616 1.4037964380158858 4 4.631388860825307 2.6099111681016556 5 4.959854909625358 3.493763185782328 6 5.650583785999834 4.776382044093934 7 5.198681179277486 5.12679216421101 8 5.04778512289935 5.689123138792006 9 4.243229254838643 5.3801389704194635 >10 28.9855926481623 60.88797761049031 >50 0.353189050269202 3.45160656637771 >100 0.07294802219307953 1.3973257652001425 >500 1.3472122549367405E-4 0.017916885022765176 >1k 5.405224318706296E-4 0.10479238240434013 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.0989907516818995E-5 0.0 0.0 2 3.0989907516818995E-5 0.0 0.0 3 3.0989907516818995E-5 2.0659938344546E-5 0.0 4 3.0989907516818995E-5 2.0659938344546E-5 0.0 5 3.0989907516818995E-5 2.0659938344546E-5 0.0 6 3.0989907516818995E-5 2.0659938344546E-5 0.0 7 3.0989907516818995E-5 2.0659938344546E-5 0.0 8 4.1319876689092E-5 2.0659938344546E-5 0.0 9 4.1319876689092E-5 2.0659938344546E-5 0.0 10-11 4.6484861275228495E-5 2.0659938344546E-5 5.1649845861365E-6 12-13 7.74747687920475E-5 6.197981503363799E-5 1.0329969172273E-5 14-15 1.7044449134250448E-4 9.296972255045699E-5 1.0329969172273E-5 16-17 2.68579198479098E-4 9.81347071365935E-5 1.0329969172273E-5 18-19 3.357239980988725E-4 1.44619568411822E-4 1.0329969172273E-5 20-21 4.8550855109683093E-4 1.44619568411822E-4 1.0329969172273E-5 22-23 7.076028883007005E-4 2.3758929096227897E-4 1.0329969172273E-5 24-25 8.78047379643205E-4 2.3758929096227897E-4 1.0329969172273E-5 26-27 0.001177616485639122 2.3758929096227897E-4 1.0329969172273E-5 28-29 0.0013738858999123088 2.3758929096227897E-4 1.0329969172273E-5 30-31 0.0016424650983914069 2.3758929096227897E-4 1.0329969172273E-5 32-33 0.0019523641735595969 2.3758929096227897E-4 1.0329969172273E-5 34-35 0.00227259321790006 2.3758929096227897E-4 1.187946454811395E-4 36-37 0.002634142138929615 2.4275427554841547E-4 2.27259321790006E-4 38-39 0.0030318459520621253 2.840741522375075E-4 2.27259321790006E-4 40-41 0.003388229888505544 3.20229044340463E-4 2.27259321790006E-4 42-43 0.0038427485320855557 3.20229044340463E-4 2.27259321790006E-4 44-45 0.004374741944457615 4.23528736063193E-4 2.27259321790006E-4 46-47 0.004875745449312856 4.23528736063193E-4 2.27259321790006E-4 48-49 0.005423233815443324 4.23528736063193E-4 2.27259321790006E-4 50-51 0.006032701996607432 5.010035048552405E-4 2.27259321790006E-4 52-53 0.006461395717256761 5.78478273647288E-4 2.27259321790006E-4 54-55 0.007045038975490185 5.78478273647288E-4 2.27259321790006E-4 56-57 0.007690662048757247 7.127678728868369E-4 2.27259321790006E-4 58-59 0.008155510661509532 7.127678728868369E-4 2.27259321790006E-4 60-61 0.008863113549810234 7.127678728868369E-4 2.27259321790006E-4 62-63 0.0096791811144198 7.2309784205911E-4 2.3242430637614248E-4 64-65 0.010562393478649141 7.2309784205911E-4 2.8923913682364394E-4 66-67 0.011528245596256667 7.2309784205911E-4 3.4088898268500896E-4 68-69 0.012680037158965107 8.057375954372939E-4 3.4088898268500896E-4 70-71 0.013723364045364679 8.057375954372939E-4 3.4088898268500896E-4 72-73 0.014658226255455386 8.057375954372939E-4 3.4088898268500896E-4 74-75 0.015613748403890638 8.057375954372939E-4 3.4088898268500896E-4 76-77 0.017080604026353405 8.057375954372939E-4 3.4088898268500896E-4 78-79 0.018185910727786615 8.057375954372939E-4 3.4088898268500896E-4 80-81 0.019260227521703005 8.057375954372939E-4 3.71878890201828E-4 82-83 0.02054114369906486 8.057375954372939E-4 5.99138211991834E-4 84-85 0.021904699629804895 8.057375954372939E-4 5.99138211991834E-4 86-87 0.02327342054513107 8.057375954372939E-4 5.99138211991834E-4 88-89 0.02466796638338792 8.057375954372939E-4 5.99138211991834E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 775 0.0 17.770752 3 CGTATGC 1235 0.0 11.932601 1 TGCCGTC 1175 0.0 11.721134 5 GCCGTCT 1210 0.0 11.382092 6 ATGCCGT 1280 0.0 11.130657 4 GTATGCC 1895 0.0 7.5185275 2 CGTCTTC 2145 3.45608E-11 6.420834 8 CCGTCTT 2225 1.2732926E-11 6.403254 7 CAACGTT 1045 0.0097758435 5.4536304 9 GCCGCGG 655 6.639103E-4 5.4381027 20-21 >>END_MODULE