FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n01 10-60155.34100000001bcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n01 10-60155.34100000001bcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9680571
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7750.017.7707523
CGTATGC12350.011.9326011
TGCCGTC11750.011.7211345
GCCGTCT12100.011.3820926
ATGCCGT12800.011.1306574
GTATGCC18950.07.51852752
CGTCTTC21453.45608E-116.4208348
CCGTCTT22251.2732926E-116.4032547
CAACGTT10450.00977584355.45363049
GCCGCGG6556.639103E-45.438102720-21