##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n01 1-58812_2015-03-25 191131308797.34100000004437.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11904405 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.794856357793606 38.0 38.0 38.0 32.0 38.0 2 36.794544792452875 38.0 38.0 38.0 32.0 38.0 3 36.77890738764348 38.0 38.0 38.0 32.0 38.0 4 36.77259552241376 38.0 38.0 38.0 32.0 38.0 5 36.75582803172439 38.0 38.0 38.0 32.0 38.0 6 38.701695968845144 40.0 38.0 40.0 38.0 40.0 7 38.64766462498546 40.0 38.0 40.0 38.0 40.0 8 38.63757987064452 40.0 38.0 40.0 38.0 40.0 9 38.63520789153259 40.0 38.0 40.0 38.0 40.0 10-11 38.6500715911463 40.0 38.0 40.0 38.0 40.0 12-13 38.641719808759866 40.0 38.0 40.0 38.0 40.0 14-15 38.626027130293366 40.0 38.0 40.0 38.0 40.0 16-17 38.606752626443736 40.0 38.0 40.0 38.0 40.0 18-19 38.57561650498282 40.0 38.0 40.0 38.0 40.0 20-21 38.551643992286884 40.0 38.0 40.0 38.0 40.0 22-23 38.53243555641798 40.0 38.0 40.0 38.0 40.0 24-25 38.489898781165465 40.0 38.0 40.0 38.0 40.0 26-27 38.41190672696368 40.0 38.0 40.0 38.0 40.0 28-29 38.371226869381545 40.0 38.0 40.0 38.0 40.0 30-31 38.32267748787109 40.0 38.0 40.0 38.0 40.0 32-33 38.28787062436132 40.0 38.0 40.0 38.0 40.0 34-35 38.2551445032322 40.0 38.0 40.0 38.0 40.0 36-37 38.21517820504259 40.0 38.0 40.0 38.0 40.0 38-39 38.16509119943416 40.0 38.0 40.0 38.0 40.0 40-41 38.11845497528016 40.0 38.0 40.0 38.0 40.0 42-43 38.08107372859038 40.0 38.0 40.0 38.0 40.0 44-45 38.05405591459632 40.0 38.0 40.0 38.0 40.0 46-47 38.017200061657846 40.0 38.0 40.0 38.0 40.0 48-49 37.97138424809975 40.0 38.0 40.0 38.0 40.0 50-51 37.91622722849231 40.0 38.0 40.0 38.0 40.0 52-53 37.87146006037261 40.0 38.0 40.0 35.0 40.0 54-55 37.81833556570026 40.0 38.0 40.0 32.0 40.0 56-57 37.7657389428535 40.0 38.0 40.0 32.0 40.0 58-59 37.74058690879553 40.0 38.0 40.0 32.0 40.0 60-61 37.67790830369094 40.0 38.0 40.0 32.0 40.0 62-63 37.64163635225784 40.0 38.0 40.0 32.0 40.0 64-65 37.594525681879944 40.0 38.0 40.0 32.0 40.0 66-67 37.50465583958207 40.0 38.0 40.0 32.0 40.0 68-69 37.44732454078974 40.0 38.0 40.0 32.0 40.0 70-71 37.42429550237917 40.0 38.0 40.0 32.0 40.0 72-73 37.37387824086966 40.0 38.0 40.0 32.0 40.0 74-75 37.299009106293006 39.0 38.0 40.0 32.0 40.0 76-77 37.22483173245534 38.0 38.0 40.0 32.0 40.0 78-79 37.13863506827934 38.0 38.0 40.0 32.0 40.0 80-81 37.08859207999056 38.0 38.0 40.0 32.0 40.0 82-83 37.00799376365303 38.0 38.0 40.0 32.0 40.0 84-85 36.93424345861889 38.0 38.0 40.0 32.0 40.0 86-87 36.873956741223104 38.0 38.0 40.0 32.0 40.0 88-89 36.82436421643921 38.0 38.0 40.0 32.0 40.0 90-91 36.75618122031298 38.0 38.0 40.0 32.0 40.0 92-93 36.683699143300316 38.0 38.0 40.0 32.0 40.0 94-95 36.59303593921746 38.0 38.0 40.0 32.0 40.0 96-97 36.5063243396037 38.0 38.0 40.0 32.0 40.0 98-99 36.39822485878126 38.0 38.0 40.0 32.0 40.0 100-101 35.537876903549574 38.0 35.0 39.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.06393447339804936 2201 2 0.050079325438247224 2201 3 0.04677887881824461 2201 4 0.008177364944202736 2201 5 0.04179096490960177 2201 6 0.12614229253659914 2201 7 0.15802798979494526 2201 8 0.14117305227772903 2201 9 0.11726404790982059 2201 10-11 0.04988674903302126 2201 12-13 -0.03228049886994455 2201 14-15 -0.0018889801547743446 2201 16-17 0.031399997971675475 2201 18-19 0.030644086248656777 2201 20-21 0.10361042084377914 2201 22-23 0.08385302751229773 2201 24-25 0.024454390819322214 2201 26-27 0.008779616384401834 2201 28-29 -0.005653105668947944 2201 30-31 -0.0038014983822804993 2201 32-33 0.04397342285957251 2201 34-35 -0.008591562132870934 2201 36-37 0.03252374584973694 2201 38-39 0.03045710167238269 2201 40-41 0.0019041089913685028 2201 42-43 -0.06948660969025866 2201 44-45 -0.10445495833702267 2201 46-47 -0.07208117415331117 2201 48-49 -0.05195159289706197 2201 50-51 -0.0797641249440062 2201 52-53 -0.09766920543051327 2201 54-55 -0.21158608543406388 2201 56-57 -0.19456897000220863 2201 58-59 -0.17049955629413915 2201 60-61 -0.07642222407445587 2201 62-63 -0.1468726883626914 2201 64-65 -0.15542344502149064 2201 66-67 -0.11242439060633558 2201 68-69 -0.10887283051929586 2201 70-71 -0.13044916089300074 2201 72-73 -0.18530213242338078 2201 74-75 -0.1332201935635382 2201 76-77 -0.13887640941027968 2201 78-79 -0.179616532099395 2201 80-81 -0.19498616987440442 2201 82-83 -0.2562740479222967 2201 84-85 -0.34760646538710915 2201 86-87 -0.36860886865454745 2201 88-89 -0.3965771308390771 2201 90-91 -0.31300822467848377 2201 92-93 -0.33460481289210975 2201 94-95 -0.2964468174705388 2201 96-97 -0.23823487614428274 2201 98-99 -0.3397527318092557 2201 100-101 -0.5454696420757443 1101 1 0.11930324204904963 1101 2 0.07648085867959509 1101 3 0.08923899198153862 1101 4 0.007540234361819387 1101 5 0.007448371452959179 1101 6 0.06841972235801563 1101 7 0.10238479466961081 1101 8 0.03941865113426246 1101 9 0.05249097167105532 1101 10-11 0.0478534981554688 1101 12-13 0.02777264314018879 1101 14-15 0.012822448996139713 1101 16-17 -0.014395585875149663 1101 18-19 -0.023123379761123886 1101 20-21 0.002792694040451238 1101 22-23 -0.019832154053496254 1101 24-25 0.00656708989114918 1101 26-27 -0.05857093250686063 1101 28-29 -0.07111209844337196 1101 30-31 0.03622542084874425 1101 32-33 -0.03872934321498178 1101 34-35 -0.02322785492974333 1101 36-37 -0.009758084085547125 1101 38-39 -0.004839264154412604 1101 40-41 -0.01014333574526205 1101 42-43 0.012016534735025175 1101 44-45 -0.042433340026065025 1101 46-47 -0.1354542212346388 1101 48-49 -0.13654893802174684 1101 50-51 -0.07928730161199837 1101 52-53 -0.13664963304081112 1101 54-55 -0.15033565631721046 1101 56-57 -0.17325184695548046 1101 58-59 -0.1057705091287815 1101 60-61 -0.1453267901459654 1101 62-63 -0.11877376510319948 1101 64-65 -0.15593716609779307 1101 66-67 -0.17596447520473646 1101 68-69 -0.18783525836757065 1101 70-71 -0.1663975564724538 1101 72-73 -0.19157760228259946 1101 74-75 -0.2718750466680575 1101 76-77 -0.21489894383564945 1101 78-79 -0.2285378329700407 1101 80-81 -0.23380669129675624 1101 82-83 -0.32147652435394747 1101 84-85 -0.3067078878282743 1101 86-87 -0.24701681191920954 1101 88-89 -0.3546053481076825 1101 90-91 -0.3477969093095794 1101 92-93 -0.41283186981404896 1101 94-95 -0.510150481270486 1101 96-97 -0.38521500927497954 1101 98-99 -0.4580676574807754 1101 100-101 -0.43636888647570515 2202 1 0.049509311529007505 2202 2 0.07327930465374521 2202 3 0.09038234123593725 2202 4 0.030834744758948318 2202 5 0.03194884450982016 2202 6 0.05497129699205772 2202 7 0.039347898344388454 2202 8 0.04575317576446736 2202 9 0.04447191717940058 2202 10-11 0.06301009100045007 2202 12-13 -0.006505905640970866 2202 14-15 -0.03444148037919348 2202 16-17 -0.005725017972963542 2202 18-19 -0.025719828620950125 2202 20-21 -0.02355762673270334 2202 22-23 -0.028451305724907172 2202 24-25 -0.006913767131777604 2202 26-27 -0.026875418475142965 2202 28-29 -0.06304693052282317 2202 30-31 -0.04639433683167482 2202 32-33 -0.09667554198020412 2202 34-35 -0.11212267564668821 2202 36-37 -0.08685957622611795 2202 38-39 -0.04310117917492562 2202 40-41 -0.07194580224793157 2202 42-43 -0.18905102401988927 2202 44-45 -0.18484706496209924 2202 46-47 -0.1953645824699919 2202 48-49 -0.1562663671875555 2202 50-51 -0.2794371517329779 2202 52-53 -0.18278765577231582 2202 54-55 -0.23310467991819195 2202 56-57 -0.27683253308350686 2202 58-59 -0.22165338139951984 2202 60-61 -0.19494123009365438 2202 62-63 -0.23262450244887134 2202 64-65 -0.2712820018148321 2202 66-67 -0.19129088766138125 2202 68-69 -0.2133690446305252 2202 70-71 -0.20688922791781295 2202 72-73 -0.3221108379580002 2202 74-75 -0.3028065654350911 2202 76-77 -0.37863003705340503 2202 78-79 -0.3590143496127851 2202 80-81 -0.4137773437214207 2202 82-83 -0.34801805244773476 2202 84-85 -0.42931371399691187 2202 86-87 -0.39609613553942324 2202 88-89 -0.4372724295126815 2202 90-91 -0.4681247999755911 2202 92-93 -0.4517531768044307 2202 94-95 -0.4851070354379914 2202 96-97 -0.5799036600729366 2202 98-99 -0.5580149739307743 2202 100-101 -0.5269039507061564 1102 1 0.1010158505026979 1102 2 0.045463058855339966 1102 3 0.041841292336805225 1102 4 -0.01856148477198616 1102 5 -0.06806987137329656 1102 6 0.005102517916235172 1102 7 -0.023619565901555006 1102 8 -0.01710951263717675 1102 9 -0.05818154413857002 1102 10-11 -0.0542695919600078 1102 12-13 -0.04673287975058571 1102 14-15 -0.05760121499211124 1102 16-17 -0.06581380260184488 1102 18-19 -0.0859869552747341 1102 20-21 -0.09457374824769715 1102 22-23 -0.11658168334749774 1102 24-25 -0.05689376114245448 1102 26-27 -0.10294643200730036 1102 28-29 -0.16680931497041485 1102 30-31 -0.16339231673051557 1102 32-33 -0.15079847202519403 1102 34-35 -0.19754002817088434 1102 36-37 -0.26672623362445336 1102 38-39 -0.1195962936727426 1102 40-41 -0.21554461289738214 1102 42-43 -0.2864745366239134 1102 44-45 -0.29236128457910127 1102 46-47 -0.3279608292745593 1102 48-49 -0.3426547078734572 1102 50-51 -0.35585259845416317 1102 52-53 -0.2924311512619653 1102 54-55 -0.32755118377841086 1102 56-57 -0.409321639675305 1102 58-59 -0.3599347883964512 1102 60-61 -0.4030914335559501 1102 62-63 -0.3642831989819939 1102 64-65 -0.4133650806734792 1102 66-67 -0.4004870362485491 1102 68-69 -0.3597780845233345 1102 70-71 -0.43368574486138556 1102 72-73 -0.47519170044239445 1102 74-75 -0.381640192880603 1102 76-77 -0.4452709233328065 1102 78-79 -0.45105294440423194 1102 80-81 -0.5609232256978203 1102 82-83 -0.5975491581402324 1102 84-85 -0.4947122104538195 1102 86-87 -0.6138797885948648 1102 88-89 -0.5490304292923582 1102 90-91 -0.6249566014574839 1102 92-93 -0.6282218706287566 1102 94-95 -0.726049732863288 1102 96-97 -0.7649722350717241 1102 98-99 -0.9030993271992784 1102 100-101 -0.8816301699568214 2203 1 0.07694008629702154 2203 2 -0.01512876461487167 2203 3 0.039107120649227056 2203 4 -0.04132183738558837 2203 5 -0.042482778617170425 2203 6 -0.0544850189659698 2203 7 -0.010655634454799667 2203 8 -0.05798536447690594 2203 9 -0.04228720392011809 2203 10-11 -0.04755014132200586 2203 12-13 -0.07236247850585897 2203 14-15 -0.08343962507313307 2203 16-17 -0.10678001179541496 2203 18-19 -0.1927115644041777 2203 20-21 -0.131276515271729 2203 22-23 -0.08125783193039382 2203 24-25 -0.08301971859390278 2203 26-27 -0.15603474987721455 2203 28-29 -0.12751782502060394 2203 30-31 -0.14369556381215176 2203 32-33 -0.11866126398508925 2203 34-35 -0.18248014410821156 2203 36-37 -0.191368446197842 2203 38-39 -0.17442722350195083 2203 40-41 -0.20102118141974046 2203 42-43 -0.16518566509427757 2203 44-45 -0.19498878068890235 2203 46-47 -0.2536608638838658 2203 48-49 -0.2916720394517327 2203 50-51 -0.29995327499452173 2203 52-53 -0.2643804749687746 2203 54-55 -0.36097262837400024 2203 56-57 -0.3187599190437709 2203 58-59 -0.2944017458206716 2203 60-61 -0.2937885204771433 2203 62-63 -0.40921987944891214 2203 64-65 -0.3732581448260177 2203 66-67 -0.4251895173332869 2203 68-69 -0.3641533399166903 2203 70-71 -0.45940668368211135 2203 72-73 -0.48716791366984324 2203 74-75 -0.45454147676055356 2203 76-77 -0.46857205395144064 2203 78-79 -0.3809394346628565 2203 80-81 -0.47245786342297436 2203 82-83 -0.538375123221094 2203 84-85 -0.601748182820451 2203 86-87 -0.5806548230389055 2203 88-89 -0.677940184622777 2203 90-91 -0.6382863845218054 2203 92-93 -0.7055099716529369 2203 94-95 -0.6425491338261224 2203 96-97 -0.7273416952360776 2203 98-99 -0.7601375813924562 2203 100-101 -0.8333457149118004 1103 1 0.09261264143020753 1103 2 0.08114702557316633 1103 3 0.09234061658421666 1103 4 0.09844913101981945 1103 5 0.0834930054530787 1103 6 0.04380595825789868 1103 7 0.05772961916132857 1103 8 0.10848856874009272 1103 9 0.05332492620878071 1103 10-11 0.0586193201153975 1103 12-13 0.02782348180910077 1103 14-15 0.041444843248648056 1103 16-17 0.049229967587322676 1103 18-19 0.018482998702566533 1103 20-21 0.006128748787844529 1103 22-23 0.024561356836990456 1103 24-25 0.040851620560715673 1103 26-27 0.060524621621596 1103 28-29 -0.003158611241367737 1103 30-31 0.028287536814993075 1103 32-33 -0.012985034252217531 1103 34-35 -0.003411803518361012 1103 36-37 -0.044768995527093125 1103 38-39 0.025014890196068507 1103 40-41 0.019133144266923807 1103 42-43 -0.022396837505851863 1103 44-45 -0.035400187997723265 1103 46-47 -0.06313496149179798 1103 48-49 -0.06689467701431084 1103 50-51 -0.10853418488311206 1103 52-53 -0.019620641968245423 1103 54-55 -0.07124249698875218 1103 56-57 -0.02828705352851557 1103 58-59 -0.02633417954314865 1103 60-61 -0.06852100308476139 1103 62-63 -0.07594076512136638 1103 64-65 -0.13201877938856654 1103 66-67 -0.11735717007765345 1103 68-69 -0.12700962278393746 1103 70-71 -0.12930081599748178 1103 72-73 -0.14782071707083588 1103 74-75 -0.1543292346115379 1103 76-77 -0.17619453581584565 1103 78-79 -0.2138810136707292 1103 80-81 -0.18935949518593986 1103 82-83 -0.2502400391654689 1103 84-85 -0.23731729881190944 1103 86-87 -0.255664880956445 1103 88-89 -0.23214234953304924 1103 90-91 -0.3415860243224671 1103 92-93 -0.294329956589543 1103 94-95 -0.36885883897279825 1103 96-97 -0.37798043415946836 1103 98-99 -0.2564939045628023 1103 100-101 -0.2428984151675735 2204 1 0.12526774158004628 2204 2 0.09085243420031475 2204 3 0.09210108400967698 2204 4 0.1103020454944712 2204 5 0.05951457972119556 2204 6 0.04482061933275361 2204 7 0.10894868102611355 2204 8 0.1284838550058538 2204 9 0.13920994682924004 2204 10-11 0.09195147076088972 2204 12-13 0.1494906377171361 2204 14-15 0.14285062514570512 2204 16-17 0.12915395224131032 2204 18-19 0.1221990293979971 2204 20-21 0.09768183062558222 2204 22-23 0.09236051942594514 2204 24-25 0.1232783088050553 2204 26-27 0.08801223530640101 2204 28-29 0.09260269512668629 2204 30-31 0.08313154539501255 2204 32-33 0.042365505061944475 2204 34-35 0.09440332961059283 2204 36-37 0.060356484248515585 2204 38-39 0.07451563426837993 2204 40-41 0.08403373467490383 2204 42-43 0.06849412633367535 2204 44-45 0.06674929183234468 2204 46-47 0.0450353098202001 2204 48-49 0.02392921396059222 2204 50-51 0.11291200409678481 2204 52-53 0.16233204371161492 2204 54-55 0.07419860728432326 2204 56-57 0.1172096540897769 2204 58-59 0.07647272156798124 2204 60-61 0.08891743971526722 2204 62-63 0.12994578703729331 2204 64-65 0.0891741952755396 2204 66-67 0.14784718391527463 2204 68-69 0.11267527173988157 2204 70-71 0.09801141103774569 2204 72-73 0.136395488475209 2204 74-75 0.06525053683191118 2204 76-77 0.09602721560018068 2204 78-79 0.12619196412452283 2204 80-81 0.14050727800771057 2204 82-83 0.06348056912747069 2204 84-85 0.060859319875071094 2204 86-87 0.06118589361900462 2204 88-89 0.08954455470922085 2204 90-91 0.046886189745727336 2204 92-93 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-0.1030035254702284 1215 6 -0.1040959898592888 1215 7 -0.1630923692582158 1215 8 -0.12334043616927204 1215 9 -0.08715162208862637 1215 10-11 -0.10910147444687368 1215 12-13 -0.15883952243333965 1215 14-15 -0.10934383453719221 1215 16-17 -0.027312919674955083 1215 18-19 -0.08520637538655507 1215 20-21 -0.0647316415730117 1215 22-23 -0.09687210541523683 1215 24-25 -0.06197421766289324 1215 26-27 -0.06075565452608345 1215 28-29 -0.04650519928918584 1215 30-31 -0.0833007214170749 1215 32-33 -0.07903941458387465 1215 34-35 -0.04199811421039357 1215 36-37 -0.028881511576116736 1215 38-39 -0.03815462782139889 1215 40-41 -0.0527475612854289 1215 42-43 0.01447774374025812 1215 44-45 -0.0425573289712986 1215 46-47 -0.04509752357047603 1215 48-49 0.04679354248100509 1215 50-51 0.03604726099332822 1215 52-53 0.02514555572768984 1215 54-55 0.09011536360411299 1215 56-57 0.05229308208988925 1215 58-59 0.08893649887891542 1215 60-61 0.11588516951213279 1215 62-63 0.14273613873826463 1215 64-65 0.14873325230148993 1215 66-67 0.23151973879146936 1215 68-69 0.22365742060363658 1215 70-71 0.17757948442440608 1215 72-73 0.1443843696643512 1215 74-75 0.19283702908199274 1215 76-77 0.17766170103317336 1215 78-79 0.23726911977857412 1215 80-81 0.2945776548461936 1215 82-83 0.21743685085360198 1215 84-85 0.25145371326843957 1215 86-87 0.34810662731065634 1215 88-89 0.32433683110375 1215 90-91 0.29867122679840463 1215 92-93 0.34337746614582443 1215 94-95 0.3600699165095591 1215 96-97 0.3720653181260545 1215 98-99 0.4179635147964831 1215 100-101 0.496897729682658 1212 1 -0.1529637469258347 1212 2 -0.0914385849535364 1212 3 -0.1417256019069839 1212 4 -0.11450271432893544 1212 5 -0.06812448118695613 1212 6 -0.10550632094350476 1212 7 -0.1377018649785029 1212 8 -0.2110127419078438 1212 9 -0.281299073981927 1212 10-11 -0.2789849980492889 1212 12-13 -0.2224557773745559 1212 14-15 -0.2087448652700985 1212 16-17 -0.08622431104146955 1212 18-19 -0.08713194966156834 1212 20-21 -0.04495085446107083 1212 22-23 -0.003377006249031922 1212 24-25 -0.0372236427214645 1212 26-27 -0.025365064937830084 1212 28-29 0.039076756776765365 1212 30-31 0.04817606572990485 1212 32-33 -0.07977398363891552 1212 34-35 -0.07023350979169152 1212 36-37 -0.1532478517948448 1212 38-39 -0.2076557483517405 1212 40-41 -0.23339828641068294 1212 42-43 -0.2614433650316954 1212 44-45 -0.05368640231889543 1212 46-47 -0.08030140761336213 1212 48-49 -0.25321089010466835 1212 50-51 -0.10256146427200008 1212 52-53 -0.20434496969470217 1212 54-55 -0.26697660675341695 1212 56-57 -0.19041115325214975 1212 58-59 -0.19080932565376685 1212 60-61 -0.23251315997282518 1212 62-63 -0.171138183406903 1212 64-65 0.015624156724371119 1212 66-67 0.013078414156623808 1212 68-69 -0.15667174718991106 1212 70-71 -0.04541793260678162 1212 72-73 -0.04065921324541222 1212 74-75 0.015668925374335174 1212 76-77 -0.10922048001500428 1212 78-79 0.11366184156601378 1212 80-81 0.12297080117220816 1212 82-83 0.17300959049340037 1212 84-85 0.2149983254408241 1212 86-87 0.14306559620813886 1212 88-89 0.16996123854415401 1212 90-91 0.26515530569734835 1212 92-93 0.3143949136224151 1212 94-95 0.21781927253205424 1212 96-97 0.29548760095459414 1212 98-99 0.29119332266834164 1212 100-101 0.2930830155058999 1213 1 -0.3285898660478992 1213 2 -0.20728751831853032 1213 3 -0.15020865917357895 1213 4 -0.13485815114902522 1213 5 -0.13188613494731527 1213 6 -0.07277409720848027 1213 7 -0.10353247371389074 1213 8 -0.19167700088309658 1213 9 -0.13094415147135408 1213 10-11 -0.17449021904386797 1213 12-13 -0.18242049032019736 1213 14-15 -0.292596950908127 1213 16-17 -0.3760521800056438 1213 18-19 -0.36336226197997945 1213 20-21 -0.22136359228875335 1213 22-23 -0.31028757535472096 1213 24-25 -0.3682019204110958 1213 26-27 -0.25205874904380465 1213 28-29 -0.21255546056515584 1213 30-31 -0.23003036606071703 1213 32-33 -0.2260043396322189 1213 34-35 -0.15136001856156156 1213 36-37 -0.05942326326692893 1213 38-39 -0.04024035847915286 1213 40-41 -0.053895700518175715 1213 42-43 0.005019923472374899 1213 44-45 -0.034074266993897595 1213 46-47 -0.0037676526817662648 1213 48-49 0.05071146918894698 1213 50-51 0.022306202685257404 1213 52-53 0.06796922959277651 1213 54-55 0.06159141926416112 1213 56-57 0.09101148258636726 1213 58-59 0.05106153431466254 1213 60-61 0.06810154155333947 1213 62-63 0.11573940889565648 1213 64-65 0.12675047874643752 1213 66-67 0.1724070775896962 1213 68-69 0.15841937407335394 1213 70-71 0.12535170786075156 1213 72-73 0.06111405517497559 1213 74-75 0.12595881672250187 1213 76-77 0.17516907412534266 1213 78-79 0.1784146894890526 1213 80-81 0.19553501663069994 1213 82-83 0.3351614592404104 1213 84-85 0.23276642019364857 1213 86-87 0.22767391638065249 1213 88-89 0.23508296988316602 1213 90-91 0.33739950843890654 1213 92-93 0.3303464716903335 1213 94-95 0.37602903039167046 1213 96-97 0.32906514094516837 1213 98-99 0.3434313559547846 1213 100-101 0.25732747261606903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 27.0 15 75.0 16 150.0 17 318.0 18 772.0 19 1805.0 20 3978.0 21 7894.0 22 14390.0 23 22990.0 24 33953.0 25 47357.0 26 63846.0 27 82565.0 28 104933.0 29 130470.0 30 157733.0 31 189884.0 32 228689.0 33 276942.0 34 340572.0 35 440333.0 36 610740.0 37 972748.0 38 2118833.0 39 6052404.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.993896663007984 30.106028958142854 31.960547763312142 19.939526615537023 2 18.705751641827355 31.35743659712936 30.92680396037874 19.010007800664543 3 19.21269479659 30.377007502684933 30.84085260876121 19.569445091963857 4 19.47319500638629 29.945797375005302 30.874898829466908 19.7061087891415 5 19.93354560769732 30.13215696206572 30.588660248034238 19.345637182202722 6 19.81984819904901 30.407937229958154 29.803799517909546 19.96841505308329 7 19.720994035401183 30.642606665347827 30.348833058015078 19.28756624123591 8 19.308272862020402 30.649394068834184 30.540148793660833 19.50218427548458 9 19.216477809293675 30.1230436011968 30.91088688399027 19.749591705519254 10-11 19.328269767998233 30.343784042057752 30.60395692614961 19.723989263794405 12-13 19.03181217372897 30.416316481168103 30.928206827640693 19.623664517462235 14-15 19.130247893076646 30.200817513688687 30.92665397961764 19.742280613617027 16-17 19.173919234098637 30.22849945041352 30.909163456720435 19.68841785876741 18-19 19.124857563229746 30.071927156376148 30.93070170243704 19.87251357795707 20-21 19.255593944754835 29.95763747659477 30.76526810697566 20.021500471674734 22-23 19.259718162677032 29.920319277308323 30.88269574614732 19.93726681386733 24-25 19.33904211054119 29.837889633536758 30.75647332208311 20.066594933838942 26-27 19.469668768004876 29.895866090395778 30.599942986698142 20.034522154901204 28-29 19.449758515729524 29.967532864369662 30.56680077769246 20.015907842208353 30-31 19.609549689294244 29.76159379063122 30.540742892221257 20.088113627853282 32-33 19.665289189548826 29.884399954031828 30.521674935113634 19.928635921305712 34-35 19.635514885460783 29.84527662668805 30.48519972284238 20.034008765008785 36-37 19.640528608159475 29.818574473758357 30.441606470399364 20.0992904476828 38-39 19.659227433593337 29.755919947015226 30.509147598956115 20.075705020435322 40-41 19.7361381655985 29.83272443584078 30.3162438773499 20.11489352121082 42-43 19.66116428605994 29.77434476185867 30.453400732328934 20.11109021975245 44-45 19.657524084197277 29.66918706456008 30.416018559950427 20.25727029129222 46-47 19.723206240587764 29.83080765118912 30.303745570867175 20.142240537355942 48-49 19.629273928754195 29.66166060081696 30.42282889027591 20.286236580152938 50-51 19.700557722940154 29.761007148483465 30.290223698707035 20.248211429869343 52-53 19.682047082982532 29.765523544305793 30.400086805169025 20.152342567542647 54-55 19.737464047305906 29.58748563110473 30.34114164433092 20.33390867725844 56-57 19.822353815722348 29.75040932068225 30.252243030290405 20.174993833304992 58-59 19.696439527551 29.652464055487194 30.404682046936237 20.24641437002557 60-61 19.758907896151158 29.67207624642374 30.19047534639852 20.378540511026582 62-63 19.720582387024702 29.654706067066343 30.35663552356549 20.26807602234346 64-65 19.75544166262473 29.620816175051186 30.322122368967737 20.30161979335635 66-67 19.79110952248745 29.660990323909818 30.178232934324644 20.369667219278085 68-69 19.66555238451983 29.599912701077553 30.37131780126004 20.36321711314258 70-71 19.80268202114377 29.63396635380928 30.155550270654246 20.407801354392703 72-73 19.7972912117881 29.65193114960255 30.219822920401896 20.330954718207455 74-75 19.770324306525108 29.576050900254973 30.249424842526633 20.404199950693286 76-77 19.768929930814856 29.70783186985343 30.19194763025747 20.331290569074245 78-79 19.806888752023916 29.586600097635323 30.25191930717569 20.354591843165064 80-81 19.873847554235198 29.611041231330006 30.126648152982305 20.388463061452487 82-83 19.759335557413042 29.73806598336541 30.255762163938105 20.246836295283448 84-85 19.808559106810794 29.58411378481714 30.199428308567867 20.4078987998042 86-87 19.721017346844818 29.752982717679078 30.18120963476117 20.344790300714937 88-89 19.780172375047496 29.638492596650234 30.20706663262615 20.374268395676115 90-91 19.792433407097228 29.584035983294672 30.105355070580302 20.5181755390278 92-93 19.6732733802448 29.59827706106507 30.3079056038152 20.42054395487493 94-95 19.75246223594207 29.497112543893 30.243759289450185 20.50666593071475 96-97 19.78064485506502 29.62011470969425 30.129577135853218 20.469663299387513 98-99 19.71215280028237 29.626288078981585 30.15745414967875 20.5041049710573 100-101 19.908979462899616 29.565151028375485 29.994036156791744 20.531833351933155 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 563.0 1 502.0 2 463.5 3 532.0 4 676.5 5 863.0 6 1092.5 7 1548.5 8 2144.5 9 2911.0 10 4020.5 11 5874.0 12 8554.0 13 12257.5 14 17213.5 15 23878.5 16 32234.5 17 42555.0 18 54481.5 19 68311.0 20 84005.5 21 99023.5 22 114384.0 23 130449.5 24 145016.0 25 157729.0 26 168849.0 27 179796.5 28 193059.5 29 206260.5 30 222422.5 31 245103.0 32 274425.0 33 310701.5 34 351768.5 35 400491.5 36 461343.5 37 521226.5 38 565634.0 39 586500.5 40 584693.0 41 559339.5 42 511998.5 43 454878.5 44 396541.5 45 352472.0 46 319715.5 47 296649.5 48 281285.5 49 266267.0 50 251870.0 51 233363.5 52 210861.0 53 191577.0 54 175761.0 55 161322.5 56 148891.0 57 135560.0 58 121577.5 59 107820.5 60 92288.5 61 75211.0 62 60244.5 63 48266.5 64 38484.5 65 30382.5 66 23562.0 67 17969.0 68 13537.0 69 10425.5 70 8002.5 71 5866.0 72 4391.5 73 3305.5 74 2546.0 75 2057.5 76 1696.0 77 1336.0 78 1068.0 79 888.5 80 651.5 81 430.0 82 287.0 83 177.5 84 103.5 85 79.5 86 44.5 87 20.5 88 18.5 89 15.5 90 7.5 91 4.0 92 2.5 93 0.5 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09441043042470414 2 0.0024444732853090936 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 5.880176287685105E-5 10-11 1.0920327391415194E-4 12-13 0.0 14-15 0.001171835131617246 16-17 0.0 18-19 0.0 20-21 1.2600377759325223E-4 22-23 1.68005036791003E-4 24-25 3.864115846193069E-4 26-27 0.0013902416794455497 28-29 0.0017934537677439571 30-31 0.0038431152165941933 32-33 0.004342930201047427 34-35 0.005955778554241057 36-37 0.007081412300740776 38-39 0.011453743383226628 40-41 0.012356770455978272 42-43 0.013079192114179584 44-45 0.015011250037276117 46-47 0.014112423090444252 48-49 0.016615698138630196 50-51 0.015208655955505546 52-53 0.015859675473070683 54-55 0.016456093353678744 56-57 0.014759242482089612 58-59 0.011810754086407511 60-61 0.006203585983507785 62-63 0.003620508542846114 64-65 3.9481183645885705E-4 66-67 0.002688080588656048 68-69 0.004015320379304971 70-71 0.003964918868267671 72-73 0.016472893857357843 74-75 0.01142854262770798 76-77 0.03314319363294512 78-79 0.024990749222661694 80-81 0.01863175858012223 82-83 0.030261907251979418 84-85 0.010966528776532721 86-87 0.015431262629253626 88-89 0.00791303723285624 90-91 0.005535765962263549 92-93 0.012495374611330847 94-95 0.012209766048786142 96-97 0.007371220989205256 98-99 0.00816084466212297 100-101 0.0014154424349642003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1904405E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.089602758123355 #Duplication Level Percentage of deduplicated Percentage of total 1 29.031543712328727 7.86452986622079 2 16.614768985822252 9.001749834878256 3 16.052543434026422 13.045710746558914 4 11.352634336969498 12.301534177869392 5 8.90088385035585 12.05607038511677 6 6.298467360080777 10.237378726175649 7 3.819869160188442 7.2435116696257795 8 2.530692066846433 5.484435423520322 9 1.5736453271341888 3.8366484114818022 >10 3.7104037290916763 16.600969943500704 >50 0.1021963123097135 1.7969362478819633 >100 0.011818257977930265 0.4173989653025939 >500 4.777820009124793E-4 0.09491823701323673 >1k 5.5684867211109385E-5 0.018207364853920764 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.008030220746018E-4 0.0 0.0 2 1.5120453311190268E-4 0.0 0.0 3 1.5120453311190268E-4 0.0 0.0 4 1.5120453311190268E-4 0.0 0.0 5 1.5120453311190268E-4 3.3601007358200596E-5 3.3601007358200596E-5 6 1.5120453311190268E-4 3.3601007358200596E-5 3.3601007358200596E-5 7 1.5960478495145283E-4 3.3601007358200596E-5 3.3601007358200596E-5 8 1.9320579230965344E-4 3.3601007358200596E-5 6.720201471640119E-5 9 1.9320579230965344E-4 3.3601007358200596E-5 6.720201471640119E-5 10-11 2.184065478283039E-4 3.3601007358200596E-5 7.140214063617627E-5 12-13 2.4780742926672943E-4 3.3601007358200596E-5 1.3860415535257747E-4 14-15 2.772083107051549E-4 3.3601007358200596E-5 2.016060441492036E-4 16-17 3.4861045134133124E-4 3.3601007358200596E-5 2.2680679966785404E-4 18-19 4.3681309565660783E-4 4.2001259197750746E-5 2.4780742926672943E-4 20-21 6.17418510206936E-4 5.04015110373009E-5 2.6880805886560477E-4 22-23 8.064241765968144E-4 5.460163695707597E-5 3.2760982174245585E-4 24-25 9.744292133878174E-4 5.880176287685105E-5 3.2760982174245585E-4 26-27 0.001167635005697471 5.880176287685105E-5 3.2760982174245585E-4 28-29 0.0013608407980071242 5.880176287685105E-5 3.2760982174245585E-4 30-31 0.001633848982792504 5.880176287685105E-5 3.4441032542155615E-4 32-33 0.0018102542714230573 5.880176287685105E-5 3.5701070318088136E-4 34-35 0.002032860945171136 5.880176287685105E-5 3.6961108094020657E-4 36-37 0.002297468878116966 5.880176287685105E-5 3.6961108094020657E-4 38-39 0.0026502794553780722 5.880176287685105E-5 3.6961108094020657E-4 40-41 0.002956888647521653 5.880176287685105E-5 3.7381120685998166E-4 42-43 0.0032550975878256833 5.880176287685105E-5 4.2841284381705765E-4 44-45 0.0036877105575625155 6.300188879662612E-5 4.536135993357081E-4 46-47 0.004128723779138898 6.720201471640119E-5 4.7881435485435856E-4 48-49 0.004410132215763829 1.0080302207460178E-4 4.8301448077413365E-4 50-51 0.004779743296704036 1.3440402943280238E-4 4.872146066939087E-4 52-53 0.005166154881323342 1.3440402943280238E-4 4.872146066939087E-4 54-55 0.005686970495375451 1.3440402943280238E-4 4.956148585334589E-4 56-57 0.006056581576315658 1.3440402943280238E-4 4.956148585334589E-4 58-59 0.006400991901737214 1.3440402943280238E-4 4.956148585334589E-4 60-61 0.006829404745554272 1.3440402943280238E-4 5.082152362927841E-4 62-63 0.007375421115125031 1.3440402943280238E-4 5.208156140521093E-4 64-65 0.007908837106936465 1.3440402943280238E-4 5.208156140521093E-4 66-67 0.00836665083219195 1.3440402943280238E-4 5.208156140521093E-4 68-69 0.008916867327682484 1.3440402943280238E-4 5.250157399718843E-4 70-71 0.009555286467488296 1.3440402943280238E-4 5.376161177312095E-4 72-73 0.010265107747930283 1.3440402943280238E-4 5.376161177312095E-4 74-75 0.010979129154292045 1.3860415535257744E-4 5.376161177312095E-4 76-77 0.01177715307904931 1.4280428127235253E-4 5.376161177312095E-4 78-79 0.01259617763340545 1.5120453311190268E-4 5.376161177312095E-4 80-81 0.01366300961702832 1.5960478495145283E-4 5.376161177312095E-4 82-83 0.014738241852490737 1.5960478495145283E-4 5.376161177312095E-4 84-85 0.01587227585083001 1.5960478495145283E-4 5.586167473300849E-4 86-87 0.017018910226928606 1.5960478495145283E-4 5.796173769289603E-4 88-89 0.018383951150855504 1.6800503679100299E-4 5.838175028487355E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 900 0.0 13.192123 3 GCCGTCT 1100 0.0 12.520524 6 ATGCCGT 1160 0.0 10.235269 4 TGCCGTC 1260 0.0 10.176782 5 CGTATGC 1525 0.0 9.040159 1 GTATGCC 2350 9.094947E-12 6.264987 2 CGTCTTC 2645 3.45608E-11 5.74585 8 CCGTCTT 2535 1.3400495E-8 5.2456255 7 >>END_MODULE