FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n02 64249-bar7.3420000000446a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n02 64249-bar7.3420000000446a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11605012
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA10150.036.97805846-47
TCGCCGT13100.029.5577444-45
GCCGTAT13800.026.85335546-47
CCGTATC14350.026.3209248-49
GAGCGTC34900.023.9535089
TGGTCGC18500.022.08524742-43
GTCGCCG18700.021.7224844-45
AGAGCGT39500.021.041658
TCGGTGG21000.020.92593638-39
GTGGTCG19500.020.83067340-41
GGTCGCC19500.020.70903442-43
TCTCGGT20800.019.98427636-37
CGTATCA19050.019.32825348-49
CTCGGTG23100.018.50862936-37
AAGAGCG48950.016.9783027
CGGTGGT25700.016.5444538-39
CGTGTAG26050.016.31899814-15
GTCGTGT25950.016.10760512-13
CGTCGTG26250.015.65209512-13
TCGTGTA26900.015.62676814-15
Produced by FastQC (version 0.11.2)