##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l01n02 64249-bar7.3420000000446a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11605012 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.965201759377756 38.0 38.0 38.0 32.0 38.0 2 35.92256931746387 38.0 38.0 38.0 32.0 38.0 3 36.137520926303225 38.0 38.0 38.0 32.0 38.0 4 35.85519265296753 38.0 38.0 38.0 32.0 38.0 5 36.066060681367674 38.0 38.0 38.0 32.0 38.0 6 38.11913068250166 40.0 38.0 40.0 38.0 40.0 7 38.17439766542249 40.0 38.0 40.0 38.0 40.0 8 38.23532341026446 40.0 38.0 40.0 38.0 40.0 9 38.23481587093576 40.0 38.0 40.0 38.0 40.0 10-11 38.21720727216827 40.0 38.0 40.0 38.0 40.0 12-13 38.223856554392185 40.0 38.0 40.0 38.0 40.0 14-15 38.168106978260774 40.0 38.0 40.0 38.0 40.0 16-17 38.182829668767255 40.0 38.0 40.0 38.0 40.0 18-19 38.16837388879908 40.0 38.0 40.0 38.0 40.0 20-21 38.14393643022515 40.0 38.0 40.0 38.0 40.0 22-23 38.13786823313927 40.0 38.0 40.0 38.0 40.0 24-25 38.078454722838714 40.0 38.0 40.0 38.0 40.0 26-27 38.07169945192646 40.0 38.0 40.0 38.0 40.0 28-29 37.965117485445084 40.0 38.0 40.0 38.0 40.0 30-31 37.95454476910494 40.0 38.0 40.0 38.0 40.0 32-33 37.9458602886408 40.0 38.0 40.0 38.0 40.0 34-35 37.88263506319511 40.0 38.0 40.0 38.0 40.0 36-37 37.88828727622169 40.0 38.0 40.0 38.0 40.0 38-39 37.866962653722375 40.0 38.0 40.0 35.0 40.0 40-41 37.78482301440102 40.0 38.0 40.0 32.0 40.0 42-43 37.61992378810121 40.0 38.0 40.0 32.0 40.0 44-45 37.5077102462281 40.0 38.0 40.0 32.0 40.0 46-47 37.45168828778462 40.0 38.0 40.0 32.0 40.0 48-49 37.42195764209464 40.0 38.0 40.0 32.0 40.0 50-51 37.34835069537196 40.0 38.0 40.0 32.0 40.0 52-53 37.306450695613236 40.0 38.0 40.0 32.0 40.0 54-55 37.28054189000407 40.0 38.0 40.0 32.0 40.0 56-57 37.22008335708744 40.0 38.0 40.0 32.0 40.0 58-59 37.157741629220205 40.0 38.0 40.0 32.0 40.0 60-61 37.115943223496885 39.0 38.0 40.0 32.0 40.0 62-63 37.08804652679377 38.0 38.0 40.0 32.0 40.0 64-65 37.01304220969354 38.0 38.0 40.0 32.0 40.0 66-67 36.99594722521614 38.0 38.0 40.0 32.0 40.0 68-69 36.935630225974776 38.0 38.0 40.0 32.0 40.0 70-71 36.826908623618834 38.0 38.0 40.0 32.0 40.0 72-73 36.78753050837001 38.0 38.0 40.0 32.0 40.0 74-75 36.678904080409396 38.0 38.0 40.0 32.0 40.0 76-77 36.61230070248958 38.0 38.0 40.0 32.0 40.0 78-79 36.575645462495 38.0 38.0 40.0 32.0 40.0 80-81 36.49365024353271 38.0 38.0 40.0 32.0 40.0 82-83 36.40753219384866 38.0 38.0 40.0 29.5 40.0 84-85 36.27526063738667 38.0 38.0 40.0 27.0 40.0 86-87 36.19621453213491 38.0 38.0 40.0 27.0 40.0 88-89 36.15532409617499 38.0 38.0 40.0 27.0 40.0 90-91 36.05159046798056 38.0 38.0 40.0 27.0 40.0 92-93 35.99093999213443 38.0 38.0 40.0 27.0 40.0 94-95 35.900716561085844 38.0 38.0 40.0 27.0 40.0 96-97 35.78750560533673 38.0 38.0 40.0 27.0 40.0 98-99 35.678473792185656 38.0 38.0 40.0 27.0 40.0 100-101 33.73753805683268 38.0 32.5 39.0 20.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.04217959168092733 2201 2 -0.016940156078597113 2201 3 -0.026785068844745297 2201 4 0.08035514140817668 2201 5 0.14768177213828437 2201 6 0.09940594067492725 2201 7 0.16434331813943714 2201 8 0.14359811875976192 2201 9 0.09119471806774015 2201 10-11 0.21770780659596767 2201 12-13 0.15168960329659598 2201 14-15 0.17015610469758968 2201 16-17 0.08214621577875647 2201 18-19 0.07628720810349421 2201 20-21 0.1260239269324117 2201 22-23 0.05965506991776692 2201 24-25 0.08472791426497395 2201 26-27 0.05079360883525652 2201 28-29 0.02093206414326687 2201 30-31 0.03969772092273871 2201 32-33 0.1352037533546806 2201 34-35 0.12050339816261868 2201 36-37 0.15293188366160848 2201 38-39 0.10016615853901101 2201 40-41 0.043496960766262305 2201 42-43 0.06006403082903944 2201 44-45 0.07850658534923838 2201 46-47 0.03802857805669646 2201 48-49 -0.041885782567213425 2201 50-51 0.02830424810598231 2201 52-53 -0.018379646724469012 2201 54-55 -0.04083002539675107 2201 56-57 0.04909726912832468 2201 58-59 0.046557761164770284 2201 60-61 -0.10903785398352284 2201 62-63 0.004228448242635352 2201 64-65 0.013738996764445233 2201 66-67 -0.026755742306420416 2201 68-69 -0.050254032166733964 2201 70-71 -0.1238191375079225 2201 72-73 -0.04701922579861417 2201 74-75 -0.01695606304922137 2201 76-77 -0.0339317956489964 2201 78-79 -0.02469348885323086 2201 80-81 -0.09025168624302893 2201 82-83 -0.08567447990522936 2201 84-85 -0.06764061323384851 2201 86-87 -0.003982551381376709 2201 88-89 -0.04024473146723295 2201 90-91 -0.09844842852439228 2201 92-93 -0.042419329928669924 2201 94-95 -0.05740466878643957 2201 96-97 -0.1429010706138527 2201 98-99 -0.12396676747229662 2201 100-101 -0.0658634639011666 1101 1 0.06304789262444643 1101 2 -0.042636472122211444 1101 3 0.16089752890393783 1101 4 -0.04711188001631683 1101 5 0.080065861898035 1101 6 0.11649334078385465 1101 7 0.11476757184847486 1101 8 0.14279421351348276 1101 9 0.13540533434164814 1101 10-11 0.179698936363188 1101 12-13 0.18743078091481635 1101 14-15 0.12907562401159822 1101 16-17 0.06919611833061623 1101 18-19 0.1060955875025158 1101 20-21 0.12118974996630527 1101 22-23 0.059062997704053544 1101 24-25 0.10032552376318193 1101 26-27 0.10742823855580497 1101 28-29 0.1350160204910651 1101 30-31 0.11571205725115163 1101 32-33 0.13404630475002222 1101 34-35 0.04380762577355313 1101 36-37 0.043219393475261825 1101 38-39 0.08692228111681999 1101 40-41 0.0015005447224325508 1101 42-43 -0.06662606240150382 1101 44-45 -0.009210532280953032 1101 46-47 -0.03460042153160714 1101 48-49 0.025823697371293974 1101 50-51 -0.024354668433140603 1101 52-53 -0.033497348439112784 1101 54-55 -0.04888033124021263 1101 56-57 -0.04738226961486447 1101 58-59 -0.04653248683437994 1101 60-61 -0.06669435446673333 1101 62-63 -0.0809313103062479 1101 64-65 -0.13268395606890238 1101 66-67 -0.18392054854847117 1101 68-69 -0.09150818261163352 1101 70-71 -0.15169810569926057 1101 72-73 -0.16806274536245525 1101 74-75 -0.15723051457598558 1101 76-77 -0.07809983712255075 1101 78-79 -0.14770491449747425 1101 80-81 -0.14455755035826456 1101 82-83 -0.12999723962199283 1101 84-85 -0.0991391740099985 1101 86-87 -0.041764078980158104 1101 88-89 -0.04055138504007516 1101 90-91 -0.02065796789888452 1101 92-93 -0.09575751244347686 1101 94-95 -0.11587270549168238 1101 96-97 -0.07804627238390083 1101 98-99 -0.07049254539528249 1101 100-101 0.22589752940506003 2202 1 0.0033188650332860448 2202 2 -0.09952560285942269 2202 3 0.15325975440656237 2202 4 0.01656606607561173 2202 5 0.11165302739402705 2202 6 0.05932270038534426 2202 7 0.11392011825222426 2202 8 0.0795699479105636 2202 9 0.11804482370747849 2202 10-11 0.09221328573384113 2202 12-13 0.09793550296480902 2202 14-15 0.08252968356024581 2202 16-17 0.0755023038581939 2202 18-19 0.11199420235657698 2202 20-21 0.10939155914565646 2202 22-23 0.050094805654197216 2202 24-25 0.08056942739107598 2202 26-27 0.027982956539240433 2202 28-29 0.05451638427904015 2202 30-31 0.06997441046578246 2202 32-33 0.1151369956814321 2202 34-35 0.07726252968608804 2202 36-37 0.05591295593572454 2202 38-39 0.07320663543534067 2202 40-41 0.05403229003319865 2202 42-43 -0.006712522826134659 2202 44-45 0.004367434523047109 2202 46-47 -0.001446457870159179 2202 48-49 -0.03255475092452542 2202 50-51 -0.03862224624757715 2202 52-53 0.028643196951563255 2202 54-55 0.04195980650116127 2202 56-57 0.01710170003065059 2202 58-59 -0.008102748542761162 2202 60-61 -0.026826434126725474 2202 62-63 3.338982184217798E-4 2202 64-65 -0.02079646236772703 2202 66-67 -0.09923001164664669 2202 68-69 -0.0785181356098974 2202 70-71 -0.03129628126004036 2202 72-73 0.026973057356563857 2202 74-75 0.08870359029157271 2202 76-77 -0.026687968723344113 2202 78-79 -0.035436000638355836 2202 80-81 0.006726979476297856 2202 82-83 -0.06041608931457887 2202 84-85 0.034646024377579465 2202 86-87 0.020625846610862197 2202 88-89 0.07594064741572737 2202 90-91 -0.001725698264877451 2202 92-93 0.01797496548267219 2202 94-95 -0.036669568797783825 2202 96-97 -0.03172244523305778 2202 98-99 0.06738443723341447 2202 100-101 0.1273569600986093 1102 1 -0.11302109590236853 1102 2 -0.07165663155851831 1102 3 0.07124198437478668 1102 4 -0.09133287201655094 1102 5 0.04404582648457023 1102 6 -0.05100823182181102 1102 7 0.07524524026575818 1102 8 0.06767781352178304 1102 9 0.06556784307322516 1102 10-11 0.12713178983748463 1102 12-13 0.0998164795098262 1102 14-15 0.11628578371380627 1102 16-17 0.10021643226171051 1102 18-19 0.08506322647068032 1102 20-21 0.10305174030572317 1102 22-23 0.11240955690497145 1102 24-25 -0.0033502588082896523 1102 26-27 -0.004476129163556664 1102 28-29 0.06943266773266998 1102 30-31 0.06904789303792569 1102 32-33 0.10430566774622463 1102 34-35 0.012058614608207563 1102 36-37 0.027307797495467412 1102 38-39 0.056272065687799966 1102 40-41 0.008668889902637034 1102 42-43 -0.09274002133655301 1102 44-45 -0.17767481516015238 1102 46-47 -0.1468432609596988 1102 48-49 -0.07575483771134373 1102 50-51 -0.08522770573295446 1102 52-53 -0.10580961930497779 1102 54-55 -0.06090128818799201 1102 56-57 -0.15058486334187648 1102 58-59 -0.14772010737573993 1102 60-61 -0.12216614878213505 1102 62-63 -0.09675462493004972 1102 64-65 -0.15986587000715247 1102 66-67 -0.16075992461462363 1102 68-69 -0.1210174850035699 1102 70-71 -0.12589196212429243 1102 72-73 -0.12435586586043712 1102 74-75 -0.1417652649397496 1102 76-77 -0.1275638605048357 1102 78-79 -0.15611568123225794 1102 80-81 -0.09605915828812073 1102 82-83 -0.09256231521867164 1102 84-85 -0.18590278357524426 1102 86-87 -0.021225548151946327 1102 88-89 -0.11982133694677799 1102 90-91 -0.08191538864702608 1102 92-93 -0.1374124933383314 1102 94-95 -0.23464718132052553 1102 96-97 -0.22060068085223605 1102 98-99 -0.1669966444567308 1102 100-101 0.11810168496580076 2203 1 -0.10735940737095717 2203 2 -0.1341553484666207 2203 3 -0.013308500922150301 2203 4 -0.16993652495266787 2203 5 -0.007001208782313029 2203 6 -0.11281468318480847 2203 7 -0.017839583798249237 2203 8 0.06550807169232797 2203 9 0.05167389226703989 2203 10-11 0.08558464247010988 2203 12-13 0.05507877023903518 2203 14-15 0.025569079245308046 2203 16-17 0.055753537527081676 2203 18-19 -0.011161389175860847 2203 20-21 0.011902635020803132 2203 22-23 0.05629734056972069 2203 24-25 0.04872263206300431 2203 26-27 0.08881410027494496 2203 28-29 0.07650248794486458 2203 30-31 0.0663583387478539 2203 32-33 0.08128930015102753 2203 34-35 0.039273544790020765 2203 36-37 0.05336878883647955 2203 38-39 0.06820734270515061 2203 40-41 0.03919855484895152 2203 42-43 -0.07861644665221235 2203 44-45 -0.13421326240360543 2203 46-47 -0.1516217119143164 2203 48-49 -0.05433873587411142 2203 50-51 -0.09426636302074343 2203 52-53 -0.1375730261051089 2203 54-55 -0.05743835573298384 2203 56-57 -0.11684835863268006 2203 58-59 -0.097632063984598 2203 60-61 -0.17477436449910044 2203 62-63 -0.17814751905876136 2203 64-65 -0.1487727070678062 2203 66-67 -0.12326089183304134 2203 68-69 -0.13089932618902367 2203 70-71 -0.1428266078558167 2203 72-73 -0.10075390111818905 2203 74-75 -0.09463242312706655 2203 76-77 -0.09516325620428745 2203 78-79 -0.15377462991863666 2203 80-81 -0.11299171971582922 2203 82-83 -0.1797521193946423 2203 84-85 -0.24373339773750047 2203 86-87 -0.055805199868466104 2203 88-89 -0.08824651352927759 2203 90-91 -0.13041303444430952 2203 92-93 -0.23077845863061697 2203 94-95 -0.15902958872647588 2203 96-97 -0.20936501977885058 2203 98-99 -0.2633742627596476 2203 100-101 -0.02883426391673538 1103 1 -0.093405172729355 1103 2 -0.13761627334292115 1103 3 -0.0217800603017011 1103 4 -0.14177684428127435 1103 5 -0.08549186160900746 1103 6 -0.1892588966938007 1103 7 -0.06808894325083514 1103 8 -0.04367696026804424 1103 9 -0.03445875198683268 1103 10-11 0.05930316636247568 1103 12-13 0.04018913541540314 1103 14-15 0.025651074557380582 1103 16-17 0.013806172424025931 1103 18-19 -0.01786971456251507 1103 20-21 0.02461173657307114 1103 22-23 -0.008657450325664229 1103 24-25 0.0042067465385216 1103 26-27 -0.014063986547867557 1103 28-29 0.0030933604052734154 1103 30-31 0.004777678857585954 1103 32-33 0.05515582124191809 1103 34-35 0.019323642526344997 1103 36-37 -0.0016709000652710415 1103 38-39 -0.05988479678564573 1103 40-41 -0.07308346785866604 1103 42-43 -0.08140818066743094 1103 44-45 -0.10169454691606461 1103 46-47 -0.13845283790161034 1103 48-49 -0.11356843659192606 1103 50-51 -0.0962204057199827 1103 52-53 -0.19127527918004716 1103 54-55 -0.10892183408500955 1103 56-57 -0.20872432900575433 1103 58-59 -0.12281527585805918 1103 60-61 -0.22602465130113103 1103 62-63 -0.12721730447353963 1103 64-65 -0.14221858919645314 1103 66-67 -0.20241544807198864 1103 68-69 -0.1524191561503443 1103 70-71 -0.2551673363018878 1103 72-73 -0.26838320939925353 1103 74-75 -0.22182930630617648 1103 76-77 -0.23440564219382054 1103 78-79 -0.2526594671042659 1103 80-81 -0.22818748808461464 1103 82-83 -0.22096341670838626 1103 84-85 -0.26297239940551265 1103 86-87 -0.1568887371848504 1103 88-89 -0.1627799227397091 1103 90-91 -0.28663145422619607 1103 92-93 -0.3632358095989119 1103 94-95 -0.35521512394215193 1103 96-97 -0.31249942653337826 1103 98-99 -0.30099152787961714 1103 100-101 -0.044525837336991 2204 1 -0.15489034979873395 2204 2 -0.008709827516085511 2204 3 -0.012428200488841412 2204 4 -0.12732716929487964 2204 5 -0.07896688157674703 2204 6 -0.17287781673061886 2204 7 -0.05177012212350718 2204 8 -0.09232759324816442 2204 9 -0.04404021141842662 2204 10-11 -0.015896056619837395 2204 12-13 -0.06336368481056098 2204 14-15 -0.05358389902912819 2204 16-17 -0.0331160822881813 2204 18-19 -0.06034445953692824 2204 20-21 -0.07541922046367944 2204 22-23 -0.08053005951353498 2204 24-25 -0.058262500182287624 2204 26-27 -0.049008749119572315 2204 28-29 -0.06562013706336245 2204 30-31 -0.0813954300814359 2204 32-33 -0.057895654970430144 2204 34-35 -0.05411165235246784 2204 36-37 -0.03890406296403626 2204 38-39 -0.09781440984506418 2204 40-41 -0.08944095377952976 2204 42-43 -0.15002129886826054 2204 44-45 -0.15365192953322548 2204 46-47 -0.20491190723826946 2204 48-49 -0.18155931422084848 2204 50-51 -0.13801910905271342 2204 52-53 -0.20804826420020106 2204 54-55 -0.14817631234866013 2204 56-57 -0.2212884665191126 2204 58-59 -0.13959412788637593 2204 60-61 -0.17801604240415259 2204 62-63 -0.11164610349565152 2204 64-65 -0.16611463625534384 2204 66-67 -0.19937863625503383 2204 68-69 -0.18129445844617464 2204 70-71 -0.20049771746154477 2204 72-73 -0.15876486543811552 2204 74-75 -0.14251485592856028 2204 76-77 -0.18380200618707931 2204 78-79 -0.18598748686370925 2204 80-81 -0.166712633723769 2204 82-83 -0.16088368547864462 2204 84-85 -0.1790580986930621 2204 86-87 -0.16771375536979605 2204 88-89 -0.13390022018774772 2204 90-91 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0.22262724974275727 1215 5 0.11785609179425904 1215 6 0.18234119820701267 1215 7 0.05233911864558394 1215 8 0.10346937873803341 1215 9 0.08279437065802142 1215 10-11 0.038341316995492036 1215 12-13 0.08434045815870661 1215 14-15 0.03842767955658388 1215 16-17 -0.026227583087930384 1215 18-19 0.02790915281720885 1215 20-21 0.07361598074839293 1215 22-23 0.05143349701052102 1215 24-25 0.011080136646228311 1215 26-27 0.034419523713374645 1215 28-29 0.2026659037362748 1215 30-31 0.1396312885732911 1215 32-33 0.12025940071431052 1215 34-35 0.08526512974633249 1215 36-37 0.044231100038004456 1215 38-39 0.13653493492815727 1215 40-41 0.1492049316723154 1215 42-43 0.20057082776350654 1215 44-45 0.2511499894439879 1215 46-47 0.281739235858133 1215 48-49 0.2088331115769222 1215 50-51 0.27527130436163105 1215 52-53 0.32443807702625804 1215 54-55 0.28354185100606344 1215 56-57 0.2554748543705756 1215 58-59 0.33238200047411937 1215 60-61 0.3755488374268694 1215 62-63 0.315635997572862 1215 64-65 0.3696393051393585 1215 66-67 0.3490328777483569 1215 68-69 0.36713492846666895 1215 70-71 0.4113990475040197 1215 72-73 0.3758200646788481 1215 74-75 0.36886629283104355 1215 76-77 0.4330716117637152 1215 78-79 0.41179107776290635 1215 80-81 0.3990937083012085 1215 82-83 0.40141823262299425 1215 84-85 0.4543943196588174 1215 86-87 0.35248470004826515 1215 88-89 0.4656215597063422 1215 90-91 0.3883585021042677 1215 92-93 0.3582294560890631 1215 94-95 0.39036850487287467 1215 96-97 0.467628826789678 1215 98-99 0.5012773252613769 1215 100-101 0.32183279168569356 1212 1 0.058251317295884064 1212 2 0.1301698703285723 1212 3 -0.03991535807602986 1212 4 0.18260884776876907 1212 5 -0.014156489147140405 1212 6 0.036030553404430066 1212 7 -0.017166205076001972 1212 8 -0.109218559343077 1212 9 -0.16785181677101235 1212 10-11 -0.05477246600087682 1212 12-13 -0.05951148106079529 1212 14-15 -0.04075322722182051 1212 16-17 0.01036017103212572 1212 18-19 -0.1259940490441167 1212 20-21 -0.021275576113772843 1212 22-23 -0.09316537692480864 1212 24-25 6.271875479910705E-4 1212 26-27 0.006322350024206003 1212 28-29 0.06786236065212137 1212 30-31 0.04403450070357451 1212 32-33 0.07771019919919553 1212 34-35 0.062380074602018 1212 36-37 0.06185275052372674 1212 38-39 0.03669447347049726 1212 40-41 0.12876222806087867 1212 42-43 0.1942338944266524 1212 44-45 0.24114511902357094 1212 46-47 0.17453363076707262 1212 48-49 0.26334600792205265 1212 50-51 0.3452980153902061 1212 52-53 0.23973769883517804 1212 54-55 0.21253260014127306 1212 56-57 0.1901938164429069 1212 58-59 0.23935712905615247 1212 60-61 0.25926093690080876 1212 62-63 0.2748951772884354 1212 64-65 0.23018239809104557 1212 66-67 0.2575512254435637 1212 68-69 0.2357989017321671 1212 70-71 0.2770406549774691 1212 72-73 0.19264896915699836 1212 74-75 0.30313755764402117 1212 76-77 0.29936450006971427 1212 78-79 0.2189699600894386 1212 80-81 0.07268951621886544 1212 82-83 0.25331027359320757 1212 84-85 0.28170414587870596 1212 86-87 0.19117513788732765 1212 88-89 0.2805284319869017 1212 90-91 0.36247961713714005 1212 92-93 0.2745385693498079 1212 94-95 0.2555955339457725 1212 96-97 0.3230856634222903 1212 98-99 0.36679605144106375 1212 100-101 -0.08383294490671744 1213 1 0.14519124569388708 1213 2 0.10650665881212262 1213 3 -0.10299980781540796 1213 4 0.16143246522798194 1213 5 -0.031826259033188364 1213 6 0.05994970461148341 1213 7 0.0027411353426884943 1213 8 -0.02716234454304356 1213 9 -0.10151898589079877 1213 10-11 -0.2799605525077524 1213 12-13 -0.15956327367771905 1213 14-15 -0.112286111293038 1213 16-17 -0.1048614557260592 1213 18-19 -0.09089980881687865 1213 20-21 -0.07423556301541367 1213 22-23 -0.034184754031834075 1213 24-25 -0.15754906687080705 1213 26-27 -0.12560659413086483 1213 28-29 -0.04468767020657083 1213 30-31 -0.21564185888660603 1213 32-33 -0.1759458966591012 1213 34-35 -0.1799317775492284 1213 36-37 -0.1566297944256263 1213 38-39 -0.251063215030193 1213 40-41 -0.13164220624171463 1213 42-43 -0.057744755102874024 1213 44-45 0.04871428952014867 1213 46-47 -0.11156538622847734 1213 48-49 -0.13413267893052705 1213 50-51 -0.22331404994591963 1213 52-53 -0.13849176019677145 1213 54-55 -0.2232653366609867 1213 56-57 -0.11603494494248423 1213 58-59 -0.05889660841237543 1213 60-61 0.031608221028690764 1213 62-63 -0.022264424962394003 1213 64-65 -0.05874118753513358 1213 66-67 -0.14036396958444186 1213 68-69 -0.026150072570089833 1213 70-71 -0.04670615305973058 1213 72-73 -0.03989363010857261 1213 74-75 -0.04398782910925547 1213 76-77 -0.05113507532793449 1213 78-79 -0.05677745430982384 1213 80-81 -0.04171481980722547 1213 82-83 -0.04211515646516517 1213 84-85 0.04778129279435461 1213 86-87 -0.06548380650015417 1213 88-89 -0.0348219641651113 1213 90-91 -0.0108090754922614 1213 92-93 -0.07355897453422955 1213 94-95 -0.18437909544083197 1213 96-97 -0.1867879386383322 1213 98-99 -0.2686641974988575 1213 100-101 -1.1369903328936957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 15.0 4 34.0 5 67.0 6 67.0 7 79.0 8 87.0 9 100.0 10 134.0 11 251.0 12 263.0 13 287.0 14 827.0 15 4633.0 16 17970.0 17 33075.0 18 37321.0 19 36272.0 20 38127.0 21 43788.0 22 51885.0 23 60107.0 24 68861.0 25 77935.0 26 87766.0 27 98092.0 28 110250.0 29 125849.0 30 144234.0 31 164822.0 32 193773.0 33 232309.0 34 288308.0 35 380657.0 36 554138.0 37 953942.0 38 2459489.0 39 5339198.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.709697146524057 33.143848613341966 33.45268822162739 17.693766018506587 2 18.80956325351831 35.279900208089444 30.392004924777677 15.518531613614575 3 18.151986261974248 33.487187660187104 31.505972374487218 16.854853703351424 4 18.54723066216724 32.09505138058076 31.29319554377799 18.064522413474005 5 18.27962114228229 32.421756221269014 31.883689108581837 17.414933527866857 6 18.470395713432673 32.63498053279951 30.767923513839385 18.126700239928432 7 18.728644599688984 32.38322583369492 31.421412514655618 17.466717051960483 8 17.9275541397025 33.05424790821673 31.31566516264599 17.702532789434777 9 18.027835693161396 32.31199264673313 31.924922359298364 17.735249300807112 10-11 17.928916600089202 32.78935083495053 31.49253060555981 17.78920195940045 12-13 17.841788235387394 32.6512497418325 31.77940738402349 17.72755463875661 14-15 17.837519644377874 32.65036431665968 31.778458439515227 17.73365759944722 16-17 17.949663516129316 32.38134287050504 31.930008527809157 17.738985085556493 18-19 17.84832510177628 32.29027199197503 31.919027698689202 17.942375207559486 20-21 18.052475300939943 32.348197669698685 31.674205785541197 17.925121243820172 22-23 18.049503970699764 32.20688946536622 31.687732302271428 18.055874261662588 24-25 18.097779951014246 32.19507678604419 31.67522708994432 18.03191617299725 26-27 18.213411406928014 32.180463442007685 31.597165594059835 18.00895955700447 28-29 18.170735092280687 32.23138796783188 31.504727000771155 18.09314993911628 30-31 18.142631789753658 32.06481117323791 31.68248678787687 18.110070249131564 32-33 18.148755617003587 32.20744124635368 31.554823144666837 18.088979991975894 34-35 18.27213958047989 32.182561550628364 31.426277090342005 18.119021778549744 36-37 18.169122122932762 32.177642462861925 31.543489563702064 18.109745850503252 38-39 18.164868900425706 32.09201109005671 31.587423855001646 18.15569615451594 40-41 18.29483829099923 32.194611748507576 31.4869584427309 18.02359151776229 42-43 18.230544329403866 32.16917065186312 31.530326643157046 18.069958375575972 44-45 18.241397980640983 32.14924823666039 31.520476810823254 18.08887697187537 46-47 18.280281249022075 32.18476879008627 31.43586537971644 18.09908458117522 48-49 18.157703989330265 32.11416079079095 31.505163680582854 18.222971539295937 50-51 18.218080044659647 32.19217443127383 31.504139962302258 18.08560556176427 52-53 18.18179271328384 32.22957877571462 31.53725389289976 18.051374618101775 54-55 18.178008834887518 32.16216764824183 31.524450140281562 18.135373376589094 56-57 18.22097579332229 32.159224721530734 31.52858213535781 18.091217349789172 58-59 18.178898582608632 32.2864693505768 31.47320190505964 18.061430161754924 60-61 18.1402252897126 32.236233757115805 31.4399365134395 18.183604439732097 62-63 18.123856087069175 32.232684453024085 31.51900000913665 18.12445945077009 64-65 18.152982194468358 32.246849482571534 31.49683916063663 18.10332916232348 66-67 18.17607629866058 32.21379188658706 31.45457897726964 18.15555283748272 68-69 18.139423244143618 32.21125949125973 31.48898179666776 18.160335467928892 70-71 18.225061245202824 32.223461083764356 31.425548336310094 18.12592933472273 72-73 18.16418527004811 32.24216097964939 31.458606960006673 18.13504679029582 74-75 18.174060944366378 32.23912547383492 31.46221614349718 18.124597438301528 76-77 18.1808056933013 32.267755911008074 31.43519843683685 18.116239958853782 78-79 18.164380791511533 32.23314978408354 31.484118171444685 18.118351252960245 80-81 18.210495605636375 32.24648692056553 31.423196442081085 18.119821031717006 82-83 18.15952506264006 32.323809705245075 31.48836179083269 18.02830344128217 84-85 18.18354431470996 32.25656823934288 31.468534693821255 18.091352752125907 86-87 18.180392599064014 32.28595909453692 31.437481863789497 18.096166442609572 88-89 18.21927327825838 32.23448383355857 31.441403524985283 18.104839363197765 90-91 18.207485928430476 32.23388159794752 31.411251910621285 18.147380563000716 92-93 18.16931290826219 32.292333184188585 31.437824314386773 18.100529593162445 94-95 18.220198160947643 32.29647656493207 31.39175698114782 18.091568292972468 96-97 18.269850351072943 32.295658924112 31.357126562654376 18.07736416216068 98-99 18.286399161182736 32.31701371535544 31.32461674691222 18.0719703765496 100-101 18.399470943947424 32.30209476504019 31.10620944236247 18.192224848649914 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10140.0 1 10924.0 2 13304.5 3 16798.5 4 20974.0 5 25901.5 6 31745.0 7 38337.5 8 45974.5 9 54665.5 10 63982.0 11 73406.0 12 83266.5 13 93414.0 14 102867.0 15 111852.0 16 120452.5 17 129150.5 18 137510.5 19 145753.5 20 154597.5 21 163647.0 22 173698.0 23 183352.0 24 193151.5 25 205205.0 26 218313.5 27 231713.5 28 246886.0 29 263551.5 30 279768.5 31 295465.0 32 311460.0 33 326752.5 34 339450.5 35 350403.5 36 360725.5 37 370133.0 38 374435.5 39 371715.0 40 363894.0 41 351482.5 42 333980.5 43 313601.0 44 294734.0 45 278500.0 46 264608.5 47 252956.5 48 239981.0 49 223952.0 50 207064.5 51 190671.0 52 174670.0 53 159823.5 54 146752.0 55 134951.0 56 123998.5 57 113134.5 58 101712.5 59 89813.5 60 77767.0 61 65840.0 62 55401.5 63 46758.5 64 39592.0 65 33733.0 66 28872.0 67 24730.5 68 21218.5 69 18077.0 70 15275.5 71 12915.5 72 10939.0 73 9238.0 74 7841.0 75 6623.5 76 5560.5 77 4681.5 78 3902.5 79 3230.5 80 2638.5 81 2219.5 82 1871.5 83 1506.5 84 1201.0 85 901.0 86 676.5 87 527.0 88 363.0 89 249.5 90 188.0 91 124.0 92 78.0 93 52.0 94 31.0 95 23.0 96 17.0 97 12.5 98 16.0 99 16.0 100 27.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02754844200074933 2 0.028177480557538416 3 0.028823753047390217 4 0.038112842968193394 5 0.022024966454149295 6 0.00978025701309055 7 0.009452812284898972 8 0.018207650280758002 9 0.007910375275786013 10-11 0.011723382965911624 12-13 0.013748370100780593 14-15 0.03486855506913737 16-17 0.025592390598131225 18-19 0.027725089814642154 20-21 0.013218426659102119 22-23 0.03693231855339745 24-25 0.04578194318110141 26-27 0.034398930393178394 28-29 0.03627742909701429 30-31 0.04685906399752107 32-33 0.04279185579472042 34-35 0.027233922722354788 36-37 0.03807406661880229 38-39 0.046807362198332925 40-41 0.027703547398313766 42-43 0.03766476070856282 44-45 0.054131783749986645 46-47 0.044678971465087675 48-49 0.056863362140426914 50-51 0.05320545984786574 52-53 0.03657471444234612 54-55 0.027285624521542932 56-57 0.03641099207825033 58-59 0.027320092387668363 60-61 0.032886652766925185 62-63 0.029177048675175864 64-65 0.030202467692407385 66-67 0.03149501267211098 68-69 0.03568716688961632 70-71 0.037117583333821626 72-73 0.030284328874455282 74-75 0.03041358337242564 76-77 0.03818608718370994 78-79 0.03245149595709164 80-81 0.03606631341699604 82-83 0.03798789695348872 84-85 0.04525630822268861 86-87 0.045583752950880185 88-89 0.045704390482319196 90-91 0.046376513871765064 92-93 0.04775522851678223 94-95 0.04697108456242872 96-97 0.05168456525508117 98-99 0.05017659611209364 100-101 0.05377848812220099 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1605012E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.74016920998729 #Duplication Level Percentage of deduplicated Percentage of total 1 97.37395112067671 92.25224605817941 2 1.9650321612192398 3.723349589139556 3 0.29463669216582666 0.8374179021378405 4 0.10227861895172932 0.38759574664202595 5 0.07393005563190017 0.35020729901349973 6 0.03741799503680215 0.212699230877106 7 0.018872725396818214 0.12516036382837273 8 0.009911599060144095 0.07512212576796819 9 0.017626698795533958 0.15029607838521292 >10 0.10584328110967015 1.7602704080652467 >50 4.184743292695692E-4 0.025540677885902655 >100 5.7820861034598186E-5 0.013585336232546782 >500 0.0 0.0 >1k 1.3608087006981981E-5 0.02062771945065123 >5k 9.148678275967352E-6 0.06588146439465813 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.89357322508585E-5 0.0 2.5850899594071942E-5 2 7.755269878221583E-5 8.616966531357313E-6 2.5850899594071942E-5 3 8.616966531357314E-5 8.616966531357313E-6 2.5850899594071942E-5 4 8.616966531357314E-5 8.616966531357313E-6 3.446786612542925E-5 5 1.0340359837628777E-4 8.616966531357313E-6 3.446786612542925E-5 6 1.0340359837628777E-4 8.616966531357313E-6 3.446786612542925E-5 7 1.292544979703597E-4 8.616966531357313E-6 6.0318765719501194E-5 8 1.37871464501717E-4 8.616966531357313E-6 6.0318765719501194E-5 9 1.37871464501717E-4 8.616966531357313E-6 6.89357322508585E-5 10-11 1.50796914298753E-4 8.616966531357313E-6 9.909511511060911E-5 12-13 2.0680719675257554E-4 8.616966531357313E-6 1.1202056490764508E-4 14-15 2.498920294093621E-4 1.292544979703597E-5 1.1632904817332373E-4 16-17 3.1021079512886325E-4 1.7233933062714626E-5 1.421799477673957E-4 18-19 4.179228767708297E-4 2.1542416328393284E-5 1.6372236409578896E-4 20-21 5.687197910695828E-4 2.5850899594071942E-5 1.895732636898609E-4 22-23 7.755269878221582E-4 3.0159382859750597E-5 2.4127506287800477E-4 24-25 9.737172180433765E-4 3.446786612542925E-5 2.714344457377554E-4 26-27 0.0011719074482645945 4.739331592246523E-5 2.8866837880047E-4 28-29 0.0014002570613455634 5.1701799188143884E-5 3.14519278394542E-4 30-31 0.0016242981911608538 5.1701799188143884E-5 3.317532114572566E-4 32-33 0.0018784987038358942 5.1701799188143884E-5 3.6191259431700716E-4 34-35 0.002093922867119827 5.1701799188143884E-5 3.7052956084836447E-4 36-37 0.0023179639969351175 5.1701799188143884E-5 3.8345501064540046E-4 38-39 0.0025807814761415154 5.1701799188143884E-5 3.877634939110791E-4 40-41 0.0028823753047390213 6.462724898517985E-5 3.963804604424364E-4 42-43 0.0030805655349602397 6.89357322508585E-5 4.049974269737937E-4 44-45 0.0033046066647755296 6.89357322508585E-5 4.2653984330218706E-4 46-47 0.003576041110513285 6.89357322508585E-5 4.308483265678657E-4 48-49 0.0038474755562510404 8.186118204789448E-5 4.351568098335443E-4 50-51 0.004252472983224834 8.616966531357314E-5 4.480822596305803E-4 52-53 0.004575609228150734 9.478663184493045E-5 4.6531619269329493E-4 54-55 0.005045233904109707 9.478663184493045E-5 4.6531619269329493E-4 56-57 0.005484699197208931 9.478663184493045E-5 4.6531619269329493E-4 58-59 0.00599740870582469 9.909511511060911E-5 4.8685860902168823E-4 60-61 0.006596287879754024 1.0340359837628777E-4 4.911670922873669E-4 62-63 0.007449367566358398 1.0340359837628777E-4 5.040925420844028E-4 64-65 0.008621275014622991 1.1202056490764508E-4 5.256349584127961E-4 66-67 0.01020248837312706 1.1202056490764508E-4 5.428688914755108E-4 68-69 0.012485984503936747 1.2494601470468104E-4 5.601028245382253E-4 70-71 0.015993089882199173 1.292544979703597E-4 5.687197910695826E-4 72-73 0.020736729957711374 1.37871464501717E-4 5.7733675760094E-4 74-75 0.027281316038277256 1.37871464501717E-4 5.816452408666187E-4 76-77 0.03662641624153426 1.37871464501717E-4 6.074961404606906E-4 78-79 0.04987931076676181 1.37871464501717E-4 6.204215902577265E-4 80-81 0.06825499189488128 1.37871464501717E-4 6.290385567890839E-4 82-83 0.09261946476229409 1.421799477673957E-4 6.462724898517985E-4 84-85 0.12489862138875857 1.5510539756443165E-4 6.548894563831558E-4 86-87 0.16721223554098869 1.5510539756443165E-4 6.635064229145131E-4 88-89 0.22087008613175066 1.5510539756443165E-4 6.807403559772277E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCGTA 1015 0.0 36.978058 46-47 TCGCCGT 1310 0.0 29.55774 44-45 GCCGTAT 1380 0.0 26.853355 46-47 CCGTATC 1435 0.0 26.32092 48-49 GAGCGTC 3490 0.0 23.953508 9 TGGTCGC 1850 0.0 22.085247 42-43 GTCGCCG 1870 0.0 21.72248 44-45 AGAGCGT 3950 0.0 21.04165 8 TCGGTGG 2100 0.0 20.925936 38-39 GTGGTCG 1950 0.0 20.830673 40-41 GGTCGCC 1950 0.0 20.709034 42-43 TCTCGGT 2080 0.0 19.984276 36-37 CGTATCA 1905 0.0 19.328253 48-49 CTCGGTG 2310 0.0 18.508629 36-37 AAGAGCG 4895 0.0 16.978302 7 CGGTGGT 2570 0.0 16.54445 38-39 CGTGTAG 2605 0.0 16.318998 14-15 GTCGTGT 2595 0.0 16.107605 12-13 CGTCGTG 2625 0.0 15.652095 12-13 TCGTGTA 2690 0.0 15.626768 14-15 >>END_MODULE