##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l01n02 61495-bar5.3420000000441a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15526190
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.48974043213435	38.0	32.0	38.0	32.0	38.0
2	35.814074798775486	38.0	38.0	38.0	32.0	38.0
3	35.763127463981824	38.0	38.0	38.0	32.0	38.0
4	35.697993776966534	38.0	32.0	38.0	32.0	38.0
5	35.62174873552365	38.0	32.0	38.0	32.0	38.0
6	37.86424505947692	40.0	38.0	40.0	38.0	40.0
7	37.960781299211206	40.0	38.0	40.0	38.0	40.0
8	38.053402219089165	40.0	38.0	40.0	38.0	40.0
9	38.05999308265582	40.0	38.0	40.0	38.0	40.0
10-11	38.043845270475245	40.0	38.0	40.0	38.0	40.0
12-13	38.0569168289194	40.0	38.0	40.0	38.0	40.0
14-15	38.002000909431096	40.0	38.0	40.0	38.0	40.0
16-17	38.01811165520968	40.0	38.0	40.0	38.0	40.0
18-19	38.01231406417157	40.0	38.0	40.0	38.0	40.0
20-21	37.99452515394955	40.0	38.0	40.0	38.0	40.0
22-23	37.991847098354455	40.0	38.0	40.0	38.0	40.0
24-25	37.93583564287182	40.0	38.0	40.0	38.0	40.0
26-27	37.93281255736275	40.0	38.0	40.0	38.0	40.0
28-29	37.83990154699897	40.0	38.0	40.0	35.0	40.0
30-31	37.836216418838106	40.0	38.0	40.0	32.0	40.0
32-33	37.82569181492691	40.0	38.0	40.0	32.0	40.0
34-35	37.78215032148904	40.0	38.0	40.0	32.0	40.0
36-37	37.79192944308939	40.0	38.0	40.0	32.0	40.0
38-39	37.774871265906185	40.0	38.0	40.0	32.0	40.0
40-41	37.68606886171044	40.0	38.0	40.0	32.0	40.0
42-43	37.514334295793105	40.0	38.0	40.0	32.0	40.0
44-45	37.39919848333686	40.0	38.0	40.0	32.0	40.0
46-47	37.34404345174187	40.0	38.0	40.0	32.0	40.0
48-49	37.31956523139289	40.0	38.0	40.0	32.0	40.0
50-51	37.248904721634865	40.0	38.0	40.0	32.0	40.0
52-53	37.21206854353837	40.0	38.0	40.0	32.0	40.0
54-55	37.190507973945955	40.0	38.0	40.0	32.0	40.0
56-57	37.133455793082526	39.0	38.0	40.0	32.0	40.0
58-59	37.075666341839174	38.0	38.0	40.0	32.0	40.0
60-61	37.03529739105344	38.0	38.0	40.0	32.0	40.0
62-63	37.01544367935726	38.0	38.0	40.0	32.0	40.0
64-65	36.942305935970126	38.0	38.0	40.0	32.0	40.0
66-67	36.93477318002678	38.0	38.0	40.0	32.0	40.0
68-69	36.887148939952425	38.0	38.0	40.0	32.0	40.0
70-71	36.78003892133228	38.0	38.0	40.0	32.0	40.0
72-73	36.74358120053922	38.0	38.0	40.0	32.0	40.0
74-75	36.64001719030877	38.0	38.0	40.0	32.0	40.0
76-77	36.57690978920134	38.0	38.0	40.0	32.0	40.0
78-79	36.5478128568567	38.0	38.0	40.0	32.0	40.0
80-81	36.464684703716756	38.0	38.0	40.0	32.0	40.0
82-83	36.380738996495595	38.0	38.0	40.0	29.5	40.0
84-85	36.25374931003678	38.0	38.0	40.0	27.0	40.0
86-87	36.17769101756451	38.0	38.0	40.0	27.0	40.0
88-89	36.139464446847555	38.0	38.0	40.0	27.0	40.0
90-91	36.03791400208293	38.0	38.0	40.0	27.0	40.0
92-93	35.98126942282685	38.0	38.0	40.0	27.0	40.0
94-95	35.892696405235284	38.0	38.0	40.0	27.0	40.0
96-97	35.77982769758711	38.0	38.0	40.0	27.0	40.0
98-99	35.676744713287675	38.0	38.0	40.0	27.0	40.0
100-101	33.72720925094953	38.0	32.5	39.0	20.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.012926609777863973
2201	2	-0.029777671822600382
2201	3	-0.02529847500652238
2201	4	0.05951433232547032
2201	5	0.0954393362796111
2201	6	0.09225689697954209
2201	7	0.12447339479999897
2201	8	0.1577076243886637
2201	9	0.09674698479824428
2201	10-11	0.2112956669354844
2201	12-13	0.16151703990456667
2201	14-15	0.20359138981008584
2201	16-17	0.1260578014152287
2201	18-19	0.1482639986189085
2201	20-21	0.15761077738780216
2201	22-23	0.120779244492212
2201	24-25	0.09519126417739443
2201	26-27	0.11873646997010923
2201	28-29	0.08782424725986715
2201	30-31	0.154389377532425
2201	32-33	0.18623100788951774
2201	34-35	0.152775550216802
2201	36-37	0.17524760484823076
2201	38-39	0.09805180987600437
2201	40-41	0.1033234687736524
2201	42-43	0.16458982581389137
2201	44-45	0.08687207963186694
2201	46-47	0.07041900553080893
2201	48-49	0.013530915966413204
2201	50-51	0.06582859028588928
2201	52-53	0.037403901398185724
2201	54-55	0.019236280747975343
2201	56-57	0.018578209373316668
2201	58-59	0.019780291427608176
2201	60-61	-0.09028122634370561
2201	62-63	0.05810926388533488
2201	64-65	0.0930687221769233
2201	66-67	-0.01648221459035426
2201	68-69	-0.03432387285513272
2201	70-71	-0.06718587787032249
2201	72-73	-0.00684959354972392
2201	74-75	-0.059353972163755486
2201	76-77	-0.018045674962785085
2201	78-79	-0.03167062394970799
2201	80-81	-0.024284835785671532
2201	82-83	-0.12637135495737084
2201	84-85	-0.08453424197099935
2201	86-87	0.014489377428617445
2201	88-89	-0.03364334607991282
2201	90-91	-0.09426707659839906
2201	92-93	-0.06930677655278572
2201	94-95	-0.03508552604738924
2201	96-97	-0.053954765623544176
2201	98-99	-0.11897843892287341
2201	100-101	0.010305852317586073
1101	1	-0.0320868034068269
1101	2	-0.00981100629367404
1101	3	0.020037399252608168
1101	4	-0.009792235604017208
1101	5	0.007317716658377549
1101	6	0.07458689030308108
1101	7	0.13309068631281207
1101	8	0.1529780496297235
1101	9	0.09801914663708544
1101	10-11	0.1461977496292235
1101	12-13	0.1464776830934369
1101	14-15	0.11830057822110263
1101	16-17	0.06190381415414947
1101	18-19	0.10341749239034215
1101	20-21	0.1229730598749228
1101	22-23	0.014585243353806732
1101	24-25	0.06898957457336508
1101	26-27	0.08289882465907539
1101	28-29	0.10454215886394991
1101	30-31	0.04743808967072738
1101	32-33	0.10003846219596113
1101	34-35	0.0617594882512833
1101	36-37	0.10748964127870408
1101	38-39	0.06520861124246835
1101	40-41	0.05570942772366294
1101	42-43	-0.02204530345435529
1101	44-45	-0.025342219166169855
1101	46-47	-0.10189513981991638
1101	48-49	0.056845978192200164
1101	50-51	0.00986059020051755
1101	52-53	-0.008128405015462192
1101	54-55	0.006029020341387081
1101	56-57	-0.011212895190645611
1101	58-59	-0.04103966452449015
1101	60-61	-0.07170343884251196
1101	62-63	0.01258819530913513
1101	64-65	-0.01960496865181227
1101	66-67	-0.12781942156592407
1101	68-69	-0.10868182341582155
1101	70-71	-0.10904794475472812
1101	72-73	-0.10152397870477614
1101	74-75	-0.052376896616138424
1101	76-77	-0.07039916930462198
1101	78-79	-0.05105514833894631
1101	80-81	-0.04500167871419336
1101	82-83	-0.06058698196383716
1101	84-85	-0.03312280546808921
1101	86-87	0.05558023334334905
1101	88-89	0.02800876874493241
1101	90-91	-0.01339329172694903
1101	92-93	-0.06913391027679694
1101	94-95	-0.0198676794415249
1101	96-97	-0.060653379243319705
1101	98-99	-0.061152331646276537
1101	100-101	0.21616037462485593
2202	1	-0.2870804399134954
2202	2	-0.08639668456410732
2202	3	0.01197399135076438
2202	4	-0.03682815916915416
2202	5	-0.10438873878401012
2202	6	-0.059232556478967524
2202	7	0.021155825254233207
2202	8	0.011033355436701697
2202	9	0.03792465158059599
2202	10-11	0.0660385246682722
2202	12-13	0.019448862364797037
2202	14-15	0.04970837921187865
2202	16-17	0.01200397873253678
2202	18-19	0.03212321881704128
2202	20-21	0.09232393580644782
2202	22-23	0.03923118612933507
2202	24-25	0.0196956267690922
2202	26-27	-0.0049422611115161885
2202	28-29	4.8559228458344705E-4
2202	30-31	-0.0404203410645394
2202	32-33	0.03710171877227708
2202	34-35	-0.005538895871239902
2202	36-37	-0.016292336231352067
2202	38-39	0.04591374023149086
2202	40-41	0.002873307781030121
2202	42-43	-0.0682041953435828
2202	44-45	-0.07914899708057987
2202	46-47	-0.10386166084045101
2202	48-49	-0.13647561300910382
2202	50-51	-0.09629909115683688
2202	52-53	-0.04980602067307416
2202	54-55	-0.05166963124965207
2202	56-57	-0.10419948529460044
2202	58-59	-0.08320660998244023
2202	60-61	-0.15644397714051195
2202	62-63	-0.12460754672241592
2202	64-65	-0.10433498502928273
2202	66-67	-0.17578136012802048
2202	68-69	-0.11234250975702054
2202	70-71	-0.09071967094557465
2202	72-73	-0.14194643943154261
2202	74-75	-0.04820500589874399
2202	76-77	-0.07375344630111869
2202	78-79	-0.07047710989024836
2202	80-81	-0.09321694169543804
2202	82-83	-0.12329570759160191
2202	84-85	-0.11809666681372732
2202	86-87	-0.09117942779981547
2202	88-89	-0.05116953234990973
2202	90-91	-0.13308441135133364
2202	92-93	-0.05265888696112597
2202	94-95	-0.09523378826202844
2202	96-97	-0.2055099263603779
2202	98-99	-0.046479971504318485
2202	100-101	0.09985166355061637
1102	1	-0.4287729242406755
1102	2	-0.14175601448886255
1102	3	-0.09610019409866766
1102	4	-0.25533170973977093
1102	5	-0.24960103770282416
1102	6	-0.2879867729903225
1102	7	-0.13409961827333916
1102	8	-0.037297991654000384
1102	9	-0.05584176270854613
1102	10-11	-0.02837202875588929
1102	12-13	7.829727322814506E-4
1102	14-15	0.021085619376528086
1102	16-17	-0.017893122247215842
1102	18-19	-0.05640022956314539
1102	20-21	-0.008277070623478266
1102	22-23	-0.021846714639380593
1102	24-25	-0.13284744855675967
1102	26-27	-0.12200584761121291
1102	28-29	-0.04946753142139215
1102	30-31	-0.027566222358039738
1102	32-33	-0.014599613579484583
1102	34-35	-0.06497620113941593
1102	36-37	-0.06015968600015498
1102	38-39	-0.04191160501556368
1102	40-41	-0.08527590827416986
1102	42-43	-0.20067316231560994
1102	44-45	-0.29070836016364154
1102	46-47	-0.24770726685228794
1102	48-49	-0.2407303607703426
1102	50-51	-0.2097847003560105
1102	52-53	-0.26721926626581194
1102	54-55	-0.19053426295469933
1102	56-57	-0.2981451803494437
1102	58-59	-0.24670485704997702
1102	60-61	-0.26657806745627965
1102	62-63	-0.24433395998403284
1102	64-65	-0.35357405761411087
1102	66-67	-0.2994381882125765
1102	68-69	-0.26944564020427464
1102	70-71	-0.27693802272122525
1102	72-73	-0.2249910524740173
1102	74-75	-0.288214302810907
1102	76-77	-0.19608615330967893
1102	78-79	-0.269780562811448
1102	80-81	-0.2438949725016002
1102	82-83	-0.2527923074214442
1102	84-85	-0.2919242005242211
1102	86-87	-0.1646148770286402
1102	88-89	-0.22119805184698293
1102	90-91	-0.17363170014096596
1102	92-93	-0.27564358429635405
1102	94-95	-0.35684360794485315
1102	96-97	-0.29718019549089547
1102	98-99	-0.28721336820101584
1102	100-101	-0.02682768398965152
2203	1	-0.29809893003452004
2203	2	-0.10787125273348863
2203	3	-0.06940494740392467
2203	4	-0.2259660069630698
2203	5	-0.1810456417656141
2203	6	-0.1763639872208671
2203	7	-0.08419678216721849
2203	8	-0.021442716422974684
2203	9	-0.005935375655816699
2203	10-11	0.03611986946283707
2203	12-13	0.03306445162206728
2203	14-15	-0.006948216366723159
2203	16-17	0.00982367673514517
2203	18-19	0.010751125538213557
2203	20-21	0.009258584455807295
2203	22-23	0.026939048105901975
2203	24-25	-0.015503251864743106
2203	26-27	-0.015657756367716047
2203	28-29	0.0044569223601342856
2203	30-31	-0.006866005508150863
2203	32-33	0.007419291375867942
2203	34-35	-0.07148360840592005
2203	36-37	-0.0030390054707467584
2203	38-39	-0.005333709264910169
2203	40-41	-0.06179624687749197
2203	42-43	-0.14758135269666184
2203	44-45	-0.19179875551450465
2203	46-47	-0.18110448627907516
2203	48-49	-0.13949300193712588
2203	50-51	-0.21711957873844767
2203	52-53	-0.21405299240264242
2203	54-55	-0.14873313397957588
2203	56-57	-0.14641229423007474
2203	58-59	-0.20285325345712124
2203	60-61	-0.1915325151899836
2203	62-63	-0.2209075394249993
2203	64-65	-0.2418200838692144
2203	66-67	-0.22657367793637917
2203	68-69	-0.22064833789821847
2203	70-71	-0.2191435947642617
2203	72-73	-0.14751502656410054
2203	74-75	-0.10954985429680875
2203	76-77	-0.16079397955375185
2203	78-79	-0.16770750961752157
2203	80-81	-0.17767600192443211
2203	82-83	-0.24012813566177726
2203	84-85	-0.249494449380407
2203	86-87	-0.15967758337357907
2203	88-89	-0.13997489155910614
2203	90-91	-0.20455394655007098
2203	92-93	-0.33545670914919157
2203	94-95	-0.24194836485143156
2203	96-97	-0.28982573002195977
2203	98-99	-0.27949292872643383
2203	100-101	-0.0783487858027172
1103	1	-0.1427900088448908
1103	2	0.037768650076799304
1103	3	-0.0355734083077337
1103	4	-0.08703875149575424
1103	5	-0.19396389762579958
1103	6	-0.08183275157610836
1103	7	0.006880879396774731
1103	8	0.05093720681136915
1103	9	0.06640145060644187
1103	10-11	0.1504059028283038
1103	12-13	0.10502605727845093
1103	14-15	0.1055738938291455
1103	16-17	0.13827387293984117
1103	18-19	0.06910244042202152
1103	20-21	0.13899762641101177
1103	22-23	0.10729828216868498
1103	24-25	0.09352693817942992
1103	26-27	0.05861635456982839
1103	28-29	0.06827309873145992
1103	30-31	0.043316047461125606
1103	32-33	0.08380416372695265
1103	34-35	0.09183461630007628
1103	36-37	0.0931898467790333
1103	38-39	0.009988605110528681
1103	40-41	-0.002298439452054879
1103	42-43	-0.032705690019682265
1103	44-45	-0.07614242163692353
1103	46-47	-0.06275562580942307
1103	48-49	-0.06687455807523435
1103	50-51	-0.10884493431717601
1103	52-53	-0.13057767499518036
1103	54-55	-0.1206665863719536
1103	56-57	-0.14752322874242196
1103	58-59	-0.1338605293806694
1103	60-61	-0.17106018882097374
1103	62-63	-0.08466146893292859
1103	64-65	-0.10336443419640062
1103	66-67	-0.14075498952681187
1103	68-69	-0.09223725435386143
1103	70-71	-0.12849642148344742
1103	72-73	-0.12898849450092342
1103	74-75	-0.17006141711323863
1103	76-77	-0.17488153521068028
1103	78-79	-0.16638201112856166
1103	80-81	-0.20752094219850648
1103	82-83	-0.1364639971502868
1103	84-85	-0.24071294230245854
1103	86-87	-0.08720472118494627
1103	88-89	-0.15785265657770964
1103	90-91	-0.23173619276629154
1103	92-93	-0.3144021049144712
1103	94-95	-0.27828881333743993
1103	96-97	-0.28846655628633755
1103	98-99	-0.24131769393797953
1103	100-101	0.04099917013441967
2204	1	-0.28309546642082495
2204	2	-0.0792544309812584
2204	3	-0.07092970542958454
2204	4	-0.16074689207257364
2204	5	-0.18624758400713404
2204	6	-0.197842297102774
2204	7	-0.11489066925528135
2204	8	-0.15178422734145158
2204	9	-0.05066205133471868
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1213	5	0.2666225656559078
1213	6	0.1929917027168102
1213	7	0.15188762495014174
1213	8	0.08492098736595466
1213	9	0.008978389138405873
1213	10-11	-0.17012313699733284
1213	12-13	-0.01130201484649973
1213	14-15	0.011552678969579233
1213	16-17	0.014161167239905126
1213	18-19	0.08106820507665446
1213	20-21	0.034943662702090705
1213	22-23	0.05603556125750231
1213	24-25	-0.03461396801028371
1213	26-27	-0.02721782300073272
1213	28-29	0.03032653560495646
1213	30-31	-0.10986444277720153
1213	32-33	-0.018482484448568925
1213	34-35	-0.03921779963823724
1213	36-37	-0.033518427333000034
1213	38-39	-0.10314143381722118
1213	40-41	-0.026368454835207444
1213	42-43	0.03123508879393455
1213	44-45	0.11349178276687866
1213	46-47	-0.033781488819890626
1213	48-49	-0.07139334770323558
1213	50-51	-0.18115361023865972
1213	52-53	-0.08963038420300506
1213	54-55	-0.06813845420842313
1213	56-57	-0.05287694106410612
1213	58-59	0.02196060885813722
1213	60-61	0.1216777936031761
1213	62-63	0.05812484876995683
1213	64-65	0.010341692735451602
1213	66-67	-0.06777216251352058
1213	68-69	0.052441628724004374
1213	70-71	0.03236644028942237
1213	72-73	0.044684857220367746
1213	74-75	0.05635845014077745
1213	76-77	0.06552712259158966
1213	78-79	0.07421333503351946
1213	80-81	0.09217163853659116
1213	82-83	0.04556385449041045
1213	84-85	0.1450127629907172
1213	86-87	0.019493759405804667
1213	88-89	0.06038987080962244
1213	90-91	0.1005125201523498
1213	92-93	0.010939040782140808
1213	94-95	-0.11632138371387413
1213	96-97	-0.09921025540103301
1213	98-99	-0.18171580547824107
1213	100-101	-1.0284832063700335
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	20.0
4	66.0
5	77.0
6	82.0
7	79.0
8	124.0
9	123.0
10	166.0
11	297.0
12	369.0
13	368.0
14	1137.0
15	7761.0
16	33750.0
17	61042.0
18	63840.0
19	57541.0
20	57017.0
21	62678.0
22	71821.0
23	81811.0
24	91726.0
25	102091.0
26	113203.0
27	126750.0
28	141207.0
29	162017.0
30	184805.0
31	215673.0
32	254982.0
33	309653.0
34	389536.0
35	519262.0
36	761925.0
37	1341549.0
38	3544115.0
39	6767527.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.391505130835004	31.971286909283954	29.373147771140534	20.26406018874051
2	21.032362773957942	33.347788171402634	28.040708655147295	17.579140399492125
3	20.702900264082068	30.942629314204833	28.998627082489087	19.355843339224016
4	20.429890814702	29.881137062119816	28.989917922402725	20.69905420077546
5	20.33596900456303	30.116336607033745	29.96874082196892	19.578953566434304
6	20.911781572713	30.015471447581653	28.745735754322766	20.32701122538258
7	20.974860549846845	30.13970039916332	29.182169627116693	19.703269423873135
8	19.97492305013477	30.32552300495142	29.741315363580668	19.958238581333145
9	20.213800869783828	29.989081486404483	29.863162058477656	19.933955585334033
10-11	20.075318468316848	30.41322222484534	29.488658347067943	20.022800959769867
12-13	20.04953063711336	30.265121476380415	29.779896186124194	19.90545170038203
14-15	20.09235801783755	30.30020076611403	29.683408966801412	19.924032249247002
16-17	20.184295224368157	30.04416606973067	29.790518468144235	19.981020237756937
18-19	20.02504833465297	29.920593300214726	29.834721578869722	20.219636786262587
20-21	20.269463987153724	29.997598264308746	29.562361371587226	20.170576376950308
22-23	20.26990719296558	29.880548820548945	29.5207887538036	20.328755232681868
24-25	20.41968827198032	29.777334597364064	29.5404142143853	20.262562916270316
26-27	20.509004147349806	29.747197572714246	29.496403086282086	20.247395193653865
28-29	20.42486440016351	29.788495922718845	29.39832031357342	20.38831936354422
30-31	20.406459818063734	29.61679884162274	29.54309218451685	20.433649155796676
32-33	20.45272297398695	29.712678010074644	29.380874080361956	20.45372493557645
34-35	20.569609305013447	29.7129002113458	29.247217892333794	20.47027259130696
36-37	20.467172596186973	29.79422353514577	29.361180837399942	20.377423031267313
38-39	20.480866168291012	29.625031328499457	29.40202750606006	20.492074997149466
40-41	20.582745418977503	29.745402452452712	29.297686341601647	20.374165786968142
42-43	20.57689340758002	29.71527484414327	29.295299190772727	20.412532557503987
44-45	20.5448203022706	29.647189216057985	29.328491726350887	20.479498755320527
46-47	20.60383575689063	29.712385890736563	29.252833368644122	20.430944983728686
48-49	20.543229301914327	29.638835159928245	29.303970077287538	20.51396546086989
50-51	20.54551233158629	29.73159425587104	29.245882017543973	20.477011394998698
52-53	20.57531933800417	29.71444526156098	29.2708892935384	20.43934610689645
54-55	20.574016382944567	29.676437875644346	29.265875342320662	20.483670399090425
56-57	20.603056599098114	29.7220536110016	29.211070872788735	20.463818917111553
58-59	20.558507435060932	29.746022613083863	29.232974433791092	20.462495518064113
60-61	20.53691532448037	29.66802261106409	29.199249734032485	20.595812330423062
62-63	20.509584104309596	29.65399155434903	29.286094099193217	20.550330242148153
64-65	20.568273267408127	29.688782593518976	29.216206728087162	20.526737410985735
66-67	20.551549024799286	29.67304902919644	29.19258317930132	20.582818766702953
68-69	20.480795374447354	29.679731765366856	29.23310035475113	20.606372505434663
70-71	20.626219252605704	29.684786707406275	29.14453427711599	20.54445976287203
72-73	20.57314444926676	29.710030570654688	29.16152020433223	20.555304775746315
74-75	20.585855389902637	29.67795432525425	29.201360859908633	20.534829424934475
76-77	20.56222805952893	29.7314376078971	29.15987917876897	20.546455153805
78-79	20.559071417671564	29.717652324498438	29.18844271069721	20.534833547132784
80-81	20.593860360373277	29.70303373873751	29.15058414456424	20.552521756324975
82-83	20.55535486824025	29.794804853483747	29.18654885904241	20.4632914192336
84-85	20.58464267496016	29.709289252839127	29.151662224897624	20.554405847303087
86-87	20.549240078031787	29.717945876942743	29.158002187052496	20.574811857972975
88-89	20.5915807083282	29.69491558406704	29.153516430033825	20.559987277570936
90-91	20.56371732575257	29.67836604461886	29.141776232582522	20.616140397046046
92-93	20.531228375929334	29.712945913971506	29.163116789739984	20.592708920359176
94-95	20.57491280779051	29.69518086845857	29.114649096664547	20.61525722708637
96-97	20.601621021854086	29.749307819679434	29.071244816843635	20.577826341622853
98-99	20.58565998148004	29.72739762307817	29.089551171223306	20.59739122421849
100-101	20.725855136360156	29.644350167510037	28.833991198279975	20.795803497849835
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	829.0
1	782.5
2	818.5
3	1021.5
4	1212.0
5	1365.0
6	1632.0
7	1929.0
8	2248.0
9	2676.5
10	3122.0
11	3721.0
12	4609.5
13	5669.5
14	6970.5
15	8953.5
16	11970.0
17	16527.5
18	23432.0
19	33665.0
20	47743.5
21	65414.0
22	87410.0
23	113158.0
24	143074.5
25	177751.5
26	214814.0
27	254065.0
28	295536.0
29	338415.5
30	380160.5
31	419644.5
32	458836.0
33	496019.5
34	530467.5
35	563472.0
36	594240.5
37	617618.5
38	630000.5
39	629321.5
40	617327.0
41	598108.0
42	569355.5
43	535090.0
44	503067.0
45	477182.5
46	455275.5
47	437157.5
48	417875.0
49	392247.0
50	365425.0
51	337851.5
52	308763.5
53	282750.0
54	260403.0
55	238689.5
56	218617.5
57	199565.0
58	179444.0
59	157533.0
60	135223.0
61	113666.5
62	94255.0
63	77903.5
64	64456.0
65	53295.0
66	43891.0
67	35796.5
68	28954.0
69	23520.5
70	19024.0
71	15358.5
72	12343.0
73	10113.0
74	8475.0
75	7186.5
76	6228.5
77	5524.0
78	4858.5
79	4241.0
80	3761.0
81	3269.0
82	2809.5
83	2380.5
84	1959.5
85	1557.0
86	1216.0
87	937.0
88	674.0
89	493.5
90	350.0
91	236.0
92	160.0
93	107.5
94	72.0
95	50.5
96	44.5
97	29.5
98	23.5
99	27.0
100	52.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028017176139155837
2	0.027579206489164437
3	0.028983285661195693
4	0.03785217107352158
5	0.021827634467953824
6	0.009422788204962068
7	0.009448551125549797
8	0.017847263237149618
9	0.0076193837638210025
10-11	0.011873486025869837
12-13	0.013274344832827628
14-15	0.034738078047479776
16-17	0.0249063034781875
18-19	0.027051066617115982
20-21	0.01288468065893822
22-23	0.03675724694854308
24-25	0.04542324936124059
26-27	0.034229260365872115
28-29	0.03616147940995183
30-31	0.04662444553364348
32-33	0.042721363064602454
34-35	0.027276492172258616
36-37	0.03723064061434261
38-39	0.0462025777090194
40-41	0.027698359996882686
42-43	0.03714047039228555
44-45	0.05371246906034256
46-47	0.04414476442707451
48-49	0.056333846230144036
50-51	0.0525177136180866
52-53	0.03534672704636488
54-55	0.027012422236234387
56-57	0.036113173933849836
58-59	0.026783776316018287
60-61	0.032380770813702525
62-63	0.028812606312301987
64-65	0.03002024321485181
66-67	0.03088652141961421
68-69	0.035101979300781454
70-71	0.036502838107739244
72-73	0.02966278269169706
74-75	0.03014583745271699
76-77	0.03802929115256222
78-79	0.03236144862326173
80-81	0.03599402042613159
82-83	0.038100139184178476
84-85	0.045313756948742734
86-87	0.04591918558255438
88-89	0.0459932539792441
90-91	0.046756480501655595
92-93	0.048044626531042064
94-95	0.047113941024810334
96-97	0.05162567249273647
98-99	0.05023769514607254
100-101	0.05354823044159578
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.552619E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.72952017539521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.42592725019438	93.2384086122927
2	0.9500728655619005	1.7999989337268318
3	0.21118172576989414	0.6001543065598189
4	0.08581468895707031	0.3251673723561623
5	0.08715737185960741	0.41281880080045513
6	0.04623172942788199	0.26277057273491755
7	0.032087425401939484	0.21277384883926606
8	0.014867857799977227	0.11267400283422799
9	0.011750469778874235	0.10018047275894147
>10	0.13033921631828504	2.6669405735811056
>50	0.004408088982540221	0.2410198871896788
>100	1.3273028312854357E-4	0.01282494555418151
>500	2.8579664526258177E-5	0.014267670771714478
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.5085111028526636E-5	6.4407301469323765E-6	0.0
2	6.440730146932377E-5	6.4407301469323765E-6	1.932219044079713E-5
3	7.084803161625615E-5	6.4407301469323765E-6	2.5762920587729506E-5
4	8.37294919101209E-5	6.4407301469323765E-6	5.152584117545901E-5
5	1.1593314264478279E-4	6.4407301469323765E-6	6.440730146932377E-5
6	1.1593314264478279E-4	6.4407301469323765E-6	8.37294919101209E-5
7	1.352553330855799E-4	6.4407301469323765E-6	9.661095220398565E-5
8	1.5457752352637704E-4	6.4407301469323765E-6	1.1593314264478279E-4
9	1.6101825367330943E-4	6.4407301469323765E-6	1.2881460293864754E-4
10-11	2.0932372977530226E-4	9.661095220398565E-6	1.416960632325123E-4
12-13	2.6729030109769365E-4	1.2881460293864753E-5	1.642386187467756E-4
14-15	3.316976025670174E-4	1.6101825367330942E-5	1.7712007904064036E-4
16-17	3.9932526910980737E-4	2.2542555514263318E-5	1.964422694814375E-4
18-19	4.44410380138334E-4	2.5762920587729506E-5	2.0932372977530223E-4
20-21	5.088176816076579E-4	3.542401580812807E-5	2.22205190069167E-4
22-23	5.98987903664711E-4	3.864438088159426E-5	2.5440884080382887E-4
24-25	6.988192209421628E-4	3.864438088159426E-5	2.73731031244626E-4
26-27	7.793283477788176E-4	3.864438088159426E-5	2.962735867588893E-4
28-29	9.242447760847961E-4	3.864438088159426E-5	3.1237541212622024E-4
30-31	0.001024076093362248	4.5085111028526636E-5	3.25256872420085E-4
32-33	0.0011206870455662335	4.5085111028526636E-5	3.8000307866901023E-4
34-35	0.001246281283431415	4.5085111028526636E-5	3.9932526910980737E-4
36-37	0.0013589940610027316	4.8305476101992824E-5	4.3474928491793544E-4
38-39	0.0015264530448229733	5.7966571322391394E-5	4.5729184043219874E-4
40-41	0.0016810305683493502	5.7966571322391394E-5	4.701733007260635E-4
42-43	0.0018323877268022612	5.7966571322391394E-5	4.95936221313793E-4
44-45	0.0019901856154021045	5.7966571322391394E-5	5.12038046681124E-4
46-47	0.0022252722657651364	5.7966571322391394E-5	5.47462062489252E-4
48-49	0.002447477455834303	5.7966571322391394E-5	5.828860782973801E-4
50-51	0.0027598528679605235	5.7966571322391394E-5	6.086489988851097E-4
52-53	0.0029820580580296905	5.7966571322391394E-5	6.311915543993729E-4
54-55	0.00327833164478858	6.440730146932377E-5	6.505137448401701E-4
56-57	0.0037001994694126506	6.440730146932377E-5	6.794970305013658E-4
58-59	0.004202576420873376	7.406839668972233E-5	7.245821415298925E-4
60-61	0.004769360673803425	8.050912683665472E-5	7.374636018237571E-4
62-63	0.005445637339231325	8.37294919101209E-5	7.471246970441557E-4
64-65	0.006324797004287594	9.339058713051947E-5	7.728876176318852E-4
66-67	0.007490569160882354	1.0305168235091802E-4	7.889894429992162E-4
68-69	0.008791596650562693	1.0305168235091802E-4	8.05091268366547E-4
70-71	0.01051127159979364	1.0305168235091802E-4	8.276338238808105E-4
72-73	0.012739764230632241	1.0305168235091802E-4	8.437356492481413E-4
74-75	0.015628431701531414	1.0627204742438422E-4	8.694985698358709E-4
76-77	0.019641006583070282	1.1271277757131661E-4	8.759392999828033E-4
78-79	0.02519291596972599	1.2559423786518136E-4	8.856003952032018E-4
80-81	0.032854164479502054	1.352553330855799E-4	9.081429507174651E-4
82-83	0.042808312921586045	1.352553330855799E-4	9.242447760847961E-4
84-85	0.05602791154816475	1.352553330855799E-4	9.371262363786608E-4
86-87	0.07313449081841714	1.352553330855799E-4	9.693298871133227E-4
88-89	0.09507161769886882	1.3847569815904608E-4	9.886520775541198E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	1670	0.0	23.18334	32-33
TTCCGAT	2085	0.0	18.227827	30-31
CCGATCT	1910	0.0	17.90746	32-33
CGATCTA	1285	0.0	16.08075	34-35
CTTCCGA	2525	0.0	15.898138	30-31
CCGATCG	255	5.638867E-11	14.903378	32-33
TCTTCCG	2795	0.0	13.853013	28-29
TCCGACG	330	1.8189894E-12	13.674418	18-19
GACGTGT	1920	0.0	12.4936695	20-21
GACCGGA	900	3.6379788E-12	11.081994	9
TCAGACG	1960	0.0	9.936353	18-19
GATCGGA	3765	0.0	9.525186	94-95
TACGATC	760	0.0	9.375891	32-33
TGACCGG	1020	3.8744474E-10	9.31203	8
GATCTGG	6970	0.0	9.267561	1
ATCTGGT	9090	0.0	9.248384	2
AGATCGG	4480	0.0	8.588127	94-95
ATCGGAA	4030	0.0	8.368445	94-95
TCCGATA	800	0.0	8.313291	32-33
TGGTGAC	8960	0.0	8.215686	5
>>END_MODULE