FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n02 61495-bar5.3420000000441a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n02 61495-bar5.3420000000441a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15526190
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC16700.023.1833432-33
TTCCGAT20850.018.22782730-31
CCGATCT19100.017.9074632-33
CGATCTA12850.016.0807534-35
CTTCCGA25250.015.89813830-31
CCGATCG2555.638867E-1114.90337832-33
TCTTCCG27950.013.85301328-29
TCCGACG3301.8189894E-1213.67441818-19
GACGTGT19200.012.493669520-21
GACCGGA9003.6379788E-1211.0819949
TCAGACG19600.09.93635318-19
GATCGGA37650.09.52518694-95
TACGATC7600.09.37589132-33
TGACCGG10203.8744474E-109.312038
GATCTGG69700.09.2675611
ATCTGGT90900.09.2483842
AGATCGG44800.08.58812794-95
ATCGGAA40300.08.36844594-95
TCCGATA8000.08.31329132-33
TGGTGAC89600.08.2156865
Produced by FastQC (version 0.11.2)