FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n02 61439-bar3.342000000043d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n02 61439-bar3.342000000043d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15107746
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA4700.025.77631446-47
TCCGATC10950.018.00392332-33
TCGCCGT6750.017.59579844-45
CGATCTA9100.013.57214934-35
GTCGCCG9650.013.29257844-45
GCCGTAT9300.013.02673946-47
CCGATCT13200.012.77578532-33
CTTCCGA19400.010.89700830-31
TTCCGAT16150.010.44250830-31
TGGTCGC12900.010.12749542-43
GTGGTCG14350.09.43492240-41
GACGTGT15900.09.26128320-21
TCCGACG3407.5299977E-69.08107918-19
TCAGACG16600.09.01374818-19
CCGTATC13600.08.90816848-49
GGTCGCC14900.08.768142-43
GAGCGTC32600.08.304729
AGATCGG58700.08.2132194-95
AGCGTCG20850.08.20127694-95
GACCGGA8158.2957595E-68.1590239
Produced by FastQC (version 0.11.2)