##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l01n02 61439-bar3.342000000043d5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15107746 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.82185502721584 38.0 38.0 38.0 32.0 38.0 2 35.97575806477022 38.0 38.0 38.0 32.0 38.0 3 35.62836143790079 38.0 32.0 38.0 32.0 38.0 4 35.92416863508296 38.0 38.0 38.0 32.0 38.0 5 35.863932779913036 38.0 38.0 38.0 32.0 38.0 6 38.02193676012292 40.0 38.0 40.0 38.0 40.0 7 38.11126378481608 40.0 38.0 40.0 38.0 40.0 8 38.16683964636419 40.0 38.0 40.0 38.0 40.0 9 38.17994663135057 40.0 38.0 40.0 38.0 40.0 10-11 38.1546168104759 40.0 38.0 40.0 38.0 40.0 12-13 38.16850160838023 40.0 38.0 40.0 38.0 40.0 14-15 38.11715175778041 40.0 38.0 40.0 38.0 40.0 16-17 38.13226331048986 40.0 38.0 40.0 38.0 40.0 18-19 38.11890837322788 40.0 38.0 40.0 38.0 40.0 20-21 38.09884641295928 40.0 38.0 40.0 38.0 40.0 22-23 38.0944093182398 40.0 38.0 40.0 38.0 40.0 24-25 38.04247986430272 40.0 38.0 40.0 38.0 40.0 26-27 38.03784323617832 40.0 38.0 40.0 38.0 40.0 28-29 37.94430774120772 40.0 38.0 40.0 38.0 40.0 30-31 37.93636410090559 40.0 38.0 40.0 38.0 40.0 32-33 37.92420424595436 40.0 38.0 40.0 38.0 40.0 34-35 37.882939420612445 40.0 38.0 40.0 38.0 40.0 36-37 37.89473519080874 40.0 38.0 40.0 38.0 40.0 38-39 37.87458930008487 40.0 38.0 40.0 38.0 40.0 40-41 37.785298117932356 40.0 38.0 40.0 35.0 40.0 42-43 37.610698214015514 40.0 38.0 40.0 32.0 40.0 44-45 37.494789196217624 40.0 38.0 40.0 32.0 40.0 46-47 37.443812200708166 40.0 38.0 40.0 32.0 40.0 48-49 37.41865216028917 40.0 38.0 40.0 32.0 40.0 50-51 37.34625158511402 40.0 38.0 40.0 32.0 40.0 52-53 37.30970294973188 40.0 38.0 40.0 32.0 40.0 54-55 37.28881350004164 40.0 38.0 40.0 32.0 40.0 56-57 37.23529055889608 40.0 38.0 40.0 32.0 40.0 58-59 37.17587835405759 39.0 38.0 40.0 32.0 40.0 60-61 37.139122176134016 38.0 38.0 40.0 32.0 40.0 62-63 37.12057824509361 38.0 38.0 40.0 32.0 40.0 64-65 37.04798372967086 38.0 38.0 40.0 32.0 40.0 66-67 37.039077801546306 38.0 38.0 40.0 32.0 40.0 68-69 36.99117651302848 38.0 38.0 40.0 32.0 40.0 70-71 36.8861373827704 38.0 38.0 40.0 32.0 40.0 72-73 36.851247796991025 38.0 38.0 40.0 32.0 40.0 74-75 36.749220631588585 38.0 38.0 40.0 32.0 40.0 76-77 36.686738213628956 38.0 38.0 40.0 32.0 40.0 78-79 36.65695256592214 38.0 38.0 40.0 32.0 40.0 80-81 36.57679302392296 38.0 38.0 40.0 32.0 40.0 82-83 36.49467703521094 38.0 38.0 40.0 32.0 40.0 84-85 36.36832330911574 38.0 38.0 40.0 32.0 40.0 86-87 36.29150523181949 38.0 38.0 40.0 27.0 40.0 88-89 36.25179924258721 38.0 38.0 40.0 27.0 40.0 90-91 36.15359713487373 38.0 38.0 40.0 27.0 40.0 92-93 36.09836020542045 38.0 38.0 40.0 27.0 40.0 94-95 36.01074458757779 38.0 38.0 40.0 27.0 40.0 96-97 35.89980543755502 38.0 38.0 40.0 27.0 40.0 98-99 35.79572286957962 38.0 38.0 40.0 27.0 40.0 100-101 33.831914006232296 38.0 32.5 39.0 20.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.04384160691400041 2201 2 0.04563875619516011 2201 3 -0.039171129965481555 2201 4 0.04254840104906776 2201 5 0.150541880041267 2201 6 0.11777404607232711 2201 7 0.11480460597952202 2201 8 0.18063796103089658 2201 9 0.14155403656175736 2201 10-11 0.22819815019053635 2201 12-13 0.18630266183289024 2201 14-15 0.21607583652584594 2201 16-17 0.12034623324250049 2201 18-19 0.1455485771705014 2201 20-21 0.1790182024636806 2201 22-23 0.10339386450785781 2201 24-25 0.13230545651416747 2201 26-27 0.13434173004968386 2201 28-29 0.09830816863799186 2201 30-31 0.10060792336978608 2201 32-33 0.19165192002168396 2201 34-35 0.16984171604756426 2201 36-37 0.16222296326969143 2201 38-39 0.11653146465121722 2201 40-41 0.17267202403434112 2201 42-43 0.13006854791730404 2201 44-45 0.1034318768556659 2201 46-47 0.08637217586579027 2201 48-49 0.04901369504876385 2201 50-51 0.09609900680753469 2201 52-53 0.02195598562251888 2201 54-55 0.0565079296047486 2201 56-57 0.016461706103726215 2201 58-59 0.0535554661154265 2201 60-61 -0.06937208892852453 2201 62-63 0.07290868792008354 2201 64-65 0.06383406615967147 2201 66-67 -0.07163187573743102 2201 68-69 -0.059942940993465754 2201 70-71 -0.08678739959599113 2201 72-73 -0.01601972005850172 2201 74-75 0.030525947615927862 2201 76-77 0.05433192642137641 2201 78-79 -0.04905920559910015 2201 80-81 -0.09515665369181647 2201 82-83 -0.09283834967959592 2201 84-85 -0.05269558729216328 2201 86-87 0.09873974330714219 2201 88-89 -0.03654933719597864 2201 90-91 -0.05534044890473666 2201 92-93 -0.012101024110272363 2201 94-95 -0.010828988280977114 2201 96-97 -0.051239018374140244 2201 98-99 -0.08515609815214731 2201 100-101 0.10730834039897985 1101 1 0.03477190700941435 1101 2 0.0504496507326877 1101 3 0.05461312462868051 1101 4 0.09167569635023654 1101 5 0.094577196524952 1101 6 0.13105738914492804 1101 7 0.176478505351767 1101 8 0.12930227735169098 1101 9 0.13375222827442457 1101 10-11 0.19782388080912483 1101 12-13 0.15907405311218525 1101 14-15 0.15756119182395878 1101 16-17 0.10914732814604378 1101 18-19 0.1408418972782357 1101 20-21 0.1578203053061742 1101 22-23 0.04979114839765941 1101 24-25 0.11790781426231689 1101 26-27 0.07907760554937937 1101 28-29 0.14075698458889718 1101 30-31 0.12649102868680018 1101 32-33 0.11809058889957669 1101 34-35 0.0876951198859075 1101 36-37 0.11871231509204705 1101 38-39 0.06727853051631882 1101 40-41 0.039877568654304696 1101 42-43 0.01635758174882085 1101 44-45 0.03265514408512615 1101 46-47 -0.057656445406607304 1101 48-49 0.0483871137287295 1101 50-51 0.027297497902935675 1101 52-53 0.05107940984321857 1101 54-55 0.03249613096501491 1101 56-57 0.04151278025236138 1101 58-59 0.056356053977403064 1101 60-61 0.02094278568779373 1101 62-63 0.11210728478324938 1101 64-65 0.009591803767321494 1101 66-67 -0.061526826994665385 1101 68-69 -0.03515343114608527 1101 70-71 -0.0124035290963036 1101 72-73 -0.025501315651695222 1101 74-75 0.04390218857370343 1101 76-77 0.009523567823123358 1101 78-79 0.08541463113171233 1101 80-81 0.029448095387827777 1101 82-83 0.02028051331261338 1101 84-85 0.04465000281280851 1101 86-87 0.06883671951712245 1101 88-89 0.01735914233788094 1101 90-91 0.07445399957527599 1101 92-93 -0.04684116616139278 1101 94-95 -0.012681094379644264 1101 96-97 0.0212558845732147 1101 98-99 -0.004344539554999471 1101 100-101 0.24663855448555694 2202 1 0.01634173108178061 2202 2 0.08634209041024121 2202 3 -0.044589960468293555 2202 4 0.11026677960988707 2202 5 0.07076124679563378 2202 6 0.06397922719038007 2202 7 0.15900727807020587 2202 8 0.1400564764804102 2202 9 0.1379306506411524 2202 10-11 0.15336156672658063 2202 12-13 0.1558425805656114 2202 14-15 0.12952705094423322 2202 16-17 0.1406358715380378 2202 18-19 0.15125393470977855 2202 20-21 0.17343756589413317 2202 22-23 0.1373024957947706 2202 24-25 0.14391367140675726 2202 26-27 0.12081131406773693 2202 28-29 0.1453672074131589 2202 30-31 0.10633223774929945 2202 32-33 0.14667824622580383 2202 34-35 0.16924927407941226 2202 36-37 0.11066534130041106 2202 38-39 0.18110039985009507 2202 40-41 0.13445194249501924 2202 42-43 0.029479010780796955 2202 44-45 0.03065817645889979 2202 46-47 0.0031729628955545763 2202 48-49 0.008403427999184032 2202 50-51 0.07057006043569913 2202 52-53 0.10689691935561996 2202 54-55 0.11841201105843879 2202 56-57 0.06646694581651502 2202 58-59 0.058490552348210656 2202 60-61 -0.012361118531323712 2202 62-63 -0.04072249434830866 2202 64-65 0.020335673252390052 2202 66-67 -0.04254293486970795 2202 68-69 -0.07257767501123169 2202 70-71 0.0395773983084311 2202 72-73 -0.026031477322625562 2202 74-75 0.023856846925454533 2202 76-77 0.0767152777943636 2202 78-79 0.014494810643142841 2202 80-81 0.006560717254032511 2202 82-83 -0.0023867348111252795 2202 84-85 0.04701652784817867 2202 86-87 0.040768823042583335 2202 88-89 0.078633298165947 2202 90-91 0.04588131896957037 2202 92-93 0.0421142649597499 2202 94-95 0.013871360255706122 2202 96-97 0.016290962817009813 2202 98-99 0.06630374378381987 2202 100-101 0.21412537197524983 1102 1 -0.1873660864031237 1102 2 -0.04069825074031286 1102 3 -0.19596075265031487 1102 4 -0.12184061155309678 1102 5 -0.09982277624008162 1102 6 -0.15375359213970086 1102 7 0.0022605109601911977 1102 8 -0.0051995169779956996 1102 9 -0.03258887620150119 1102 10-11 0.06817300962313766 1102 12-13 0.04903592847627891 1102 14-15 0.04512336431467645 1102 16-17 0.04961809320879951 1102 18-19 -0.012335331289946794 1102 20-21 0.053883719208350556 1102 22-23 0.013622984119322723 1102 24-25 -0.09581455983997245 1102 26-27 -0.081519834994225 1102 28-29 -0.007510363932077269 1102 30-31 0.02549497025395908 1102 32-33 0.05711769997743943 1102 34-35 -0.02190159327366814 1102 36-37 -0.016739907266021703 1102 38-39 -0.02042838115556833 1102 40-41 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2203 2 2.3626054940706354E-4 2203 3 -0.27755444402876606 2203 4 -0.12029509350243472 2203 5 -0.1160236502646157 2203 6 -0.21984201027379413 2203 7 -0.061825499222941005 2203 8 -0.020642457669168834 2203 9 -0.027181153338226238 2203 10-11 0.030252252283005987 2203 12-13 0.0013173787920166546 2203 14-15 -0.020635915915562464 2203 16-17 -0.008420914798641377 2203 18-19 -0.020978315361787736 2203 20-21 0.010532860398683397 2203 22-23 0.022659901695561757 2203 24-25 -0.02200259942674876 2203 26-27 -0.0036291198501672284 2203 28-29 0.010498072248680046 2203 30-31 0.004907886169242204 2203 32-33 -0.006458401217422249 2203 34-35 -0.024123026042161655 2203 36-37 -0.02983317187572254 2203 38-39 -0.052378816178006105 2203 40-41 -0.06095884387693218 2203 42-43 -0.1574250089653404 2203 44-45 -0.24705188369785702 2203 46-47 -0.1920888839841126 2203 48-49 -0.18399964523909063 2203 50-51 -0.1783881252370989 2203 52-53 -0.224390365668377 2203 54-55 -0.17991463954346187 2203 56-57 -0.1892096813760702 2203 58-59 -0.2047826393806318 2203 60-61 -0.2349215602091803 2203 62-63 -0.22935828472314057 2203 64-65 -0.2426406074704488 2203 66-67 -0.20068232559051324 2203 68-69 -0.20870711829354605 2203 70-71 -0.2569419311551968 2203 72-73 -0.14789254834074228 2203 74-75 -0.1902152459996458 2203 76-77 -0.1950085662723282 2203 78-79 -0.20909099629595573 2203 80-81 -0.23714692043338914 2203 82-83 -0.22198250555238985 2203 84-85 -0.2643041731105811 2203 86-87 -0.13793660861725243 2203 88-89 -0.17437737803340525 2203 90-91 -0.2385826716051085 2203 92-93 -0.31857581634677246 2203 94-95 -0.2344095908069903 2203 96-97 -0.2523281249778435 2203 98-99 -0.30875015203739764 2203 100-101 -0.047404888901198206 1103 1 -0.2035821327126044 1103 2 -0.07210029161234388 1103 3 -0.23222063151270333 1103 4 -0.02135752019295012 1103 5 -0.18054085736140024 1103 6 -0.21198832675843704 1103 7 -0.09153842795731748 1103 8 -0.09248221933417966 1103 9 -0.06703140492819415 1103 10-11 0.02920224659605708 1103 12-13 -0.015421672500643524 1103 14-15 0.008316674715317163 1103 16-17 0.009530227941134228 1103 18-19 -0.07912335276810722 1103 20-21 -0.020040190666293256 1103 22-23 -0.0257907685223131 1103 24-25 -0.04216143147394291 1103 26-27 -0.0470866111736683 1103 28-29 -0.05148161851892752 1103 30-31 -0.03540313157327546 1103 32-33 -0.01253329094507194 1103 34-35 -0.06559965627746323 1103 36-37 -0.031838094702166586 1103 38-39 -0.12710244502174817 1103 40-41 -0.09223660475578299 1103 42-43 -0.1230438589178462 1103 44-45 -0.17432126265198633 1103 46-47 -0.22130143323309426 1103 48-49 -0.1353010017634375 1103 50-51 -0.1384367965566824 1103 52-53 -0.23978843014149476 1103 54-55 -0.20377055394934018 1103 56-57 -0.2701628822549935 1103 58-59 -0.17847763429249852 1103 60-61 -0.2858643951787343 1103 62-63 -0.1603267173939713 1103 64-65 -0.14419890413900305 1103 66-67 -0.2056215165802726 1103 68-69 -0.15510743260538362 1103 70-71 -0.2475450321704642 1103 72-73 -0.24605236816035614 1103 74-75 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-0.034123920954030496 1215 16-17 -0.059958401685968 1215 18-19 -0.07956667059730904 1215 20-21 -0.011347596352862865 1215 22-23 -0.00951761450653521 1215 24-25 -0.06514510236294768 1215 26-27 -0.030172281347489616 1215 28-29 0.09166842098746031 1215 30-31 0.05090384623874655 1215 32-33 0.05902265909045923 1215 34-35 0.04080691036990203 1215 36-37 -0.07024337172224193 1215 38-39 0.02612372078063885 1215 40-41 0.0468655403499838 1215 42-43 0.10979444422505225 1215 44-45 0.22703332238182128 1215 46-47 0.2152916098548303 1215 48-49 0.17293256014163205 1215 50-51 0.2157398695173569 1215 52-53 0.2357923012309584 1215 54-55 0.21130294095789282 1215 56-57 0.18616645497095874 1215 58-59 0.3052415609995549 1215 60-61 0.33876817437496953 1215 62-63 0.25161324894047965 1215 64-65 0.3225743568225994 1215 66-67 0.26150109333141813 1215 68-69 0.24496100671297683 1215 70-71 0.308790696060953 1215 72-73 0.32680905449925746 1215 74-75 0.2927133559750672 1215 76-77 0.30446733339694276 1215 78-79 0.31114589728279896 1215 80-81 0.3449236259054089 1215 82-83 0.3283126033454167 1215 84-85 0.3526003660584607 1215 86-87 0.3368405262985732 1215 88-89 0.38216152128003955 1215 90-91 0.3062776786969934 1215 92-93 0.28959732896236545 1215 94-95 0.32072415867204995 1215 96-97 0.42823400258647126 1215 98-99 0.4104218322770592 1215 100-101 0.19933598265678398 1212 1 0.08216356009207004 1212 2 -0.0017932128343929321 1212 3 0.16687516535322544 1212 4 0.08415608835947097 1212 5 0.04836390872522145 1212 6 0.03992315338238939 1212 7 0.0026227399138178953 1212 8 -0.08248237968388139 1212 9 -0.08919068422285648 1212 10-11 -0.04396048277862974 1212 12-13 -0.053821621581569445 1212 14-15 0.024973328068710998 1212 16-17 0.028101974529292306 1212 18-19 -0.10590110505295058 1212 20-21 0.0030534126567900444 1212 22-23 -0.030492541741551804 1212 24-25 -0.0097071818114145 1212 26-27 0.012041006698268575 1212 28-29 0.0631482614965222 1212 30-31 0.04933124446048254 1212 32-33 0.11133900114096207 1212 34-35 0.06877139844333868 1212 36-37 0.03213915537390477 1212 38-39 0.07072185664341646 1212 40-41 0.10805896358078826 1212 42-43 0.17963211606750207 1212 44-45 0.19255928955404755 1212 46-47 0.2150034648220327 1212 48-49 0.2586331196497156 1212 50-51 0.3249649067327738 1212 52-53 0.2781350862537053 1212 54-55 0.20710976102671452 1212 56-57 0.17167799899247882 1212 58-59 0.20582274363280817 1212 60-61 0.2626010977818325 1212 62-63 0.2599132941987179 1212 64-65 0.26996690929503586 1212 66-67 0.2719590621911294 1212 68-69 0.24094270191633882 1212 70-71 0.1935242967552 1212 72-73 0.1642958117342843 1212 74-75 0.289535464266919 1212 76-77 0.2811329488029557 1212 78-79 0.2567377412551082 1212 80-81 0.15984457751010694 1212 82-83 0.2565851255393383 1212 84-85 0.24090989510091987 1212 86-87 0.2306947543325606 1212 88-89 0.3055565183064033 1212 90-91 0.3627513598732932 1212 92-93 0.3077716884656496 1212 94-95 0.31233096910449376 1212 96-97 0.31855650419120707 1212 98-99 0.31840074099412874 1212 100-101 -0.07032138914671293 1213 1 0.1991952807876558 1213 2 0.008066598177187245 1213 3 0.2449502120538014 1213 4 0.06875690449734151 1213 5 0.11560227635687426 1213 6 0.0911888063534434 1213 7 0.0405011208324666 1213 8 0.03692433605772294 1213 9 -0.04776919470602792 1213 10-11 -0.22144037324741817 1213 12-13 -0.12273538983809118 1213 14-15 -0.07315284081632711 1213 16-17 -0.12069786650016567 1213 18-19 -0.03262977489561081 1213 20-21 -0.059818925884059126 1213 22-23 -0.050778990487287956 1213 24-25 -0.15462915138827782 1213 26-27 -0.10700118600551178 1213 28-29 0.004429097958890793 1213 30-31 -0.1959173863875705 1213 32-33 -0.07757886936721547 1213 34-35 -0.10661392789469915 1213 36-37 -0.08742724399888147 1213 38-39 -0.14923800372108076 1213 40-41 -0.07936440515213405 1213 42-43 -0.055912739688686486 1213 44-45 0.04370758353109494 1213 46-47 -0.1538898662231034 1213 48-49 -0.12262345160607424 1213 50-51 -0.22288776484550965 1213 52-53 -0.13649793499197216 1213 54-55 -0.12841513213837175 1213 56-57 -0.07860352623622902 1213 58-59 -0.013925922272477465 1213 60-61 0.07691480267242667 1213 62-63 -0.006404845882222787 1213 64-65 -0.026102520202414325 1213 66-67 -0.13009632853950137 1213 68-69 0.05633401019265705 1213 70-71 -0.022392402298322622 1213 72-73 -0.018078953354859095 1213 74-75 -0.01162395031291652 1213 76-77 -0.01211160809067735 1213 78-79 0.012565051593327325 1213 80-81 0.04637246656665184 1213 82-83 -0.031281737931223574 1213 84-85 0.03683974612436458 1213 86-87 -0.07641274904385398 1213 88-89 0.004703542985275533 1213 90-91 0.016192412703681214 1213 92-93 -0.04971852566292512 1213 94-95 -0.18844571190489745 1213 96-97 -0.14120551415064853 1213 98-99 -0.13441143121038834 1213 100-101 -1.0539262907098532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 26.0 4 65.0 5 90.0 6 113.0 7 104.0 8 112.0 9 124.0 10 175.0 11 283.0 12 347.0 13 371.0 14 831.0 15 5608.0 16 25478.0 17 48825.0 18 54700.0 19 51512.0 20 52353.0 21 58125.0 22 66987.0 23 75853.0 24 85200.0 25 94859.0 26 105208.0 27 117775.0 28 133080.0 29 151238.0 30 174433.0 31 203111.0 32 241096.0 33 293752.0 34 369900.0 35 495113.0 36 729808.0 37 1288390.0 38 3422003.0 39 6760698.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.991369454255963 32.20320713707043 30.056779975905023 19.748643432768585 2 20.757146749322757 33.43581258572077 27.685116308787112 18.121924356169362 3 20.504208735705486 30.887184748706048 28.838427082525016 19.77017943306345 4 20.314203624820305 29.782810079996082 29.493457484742073 20.40952881044154 5 20.454127004797858 30.117707672035525 29.898090318133335 19.530075005033286 6 20.42595470855126 30.161846716386187 29.128271577372132 20.28392699769042 7 20.740134757116326 30.211183650410785 29.488821797704656 19.559859794768233 8 19.94150351420495 30.456233264055317 29.793041819247463 19.809221402492273 9 20.119743894280727 30.111532899517783 29.97645925542085 19.792263950780633 10-11 20.026663058368047 30.45346992791369 29.610691014561908 19.909175999156357 12-13 19.931842041048757 30.317740135046193 29.873919327912223 19.876498495992823 14-15 19.99508357182976 30.330915344569608 29.803668979705417 19.87033210389522 16-17 20.107327046409075 30.046210118158733 29.91249904411694 19.93396379131525 18-19 19.95719408739194 29.962655727760435 29.933569794751836 20.146580390095792 20-21 20.18556295638951 30.01308119376485 29.70683495684232 20.094520893003313 22-23 20.197922136725285 29.899695078840193 29.64798174929375 20.254401035140774 24-25 20.338716536181586 29.840415075563868 29.610052627454024 20.210815760800518 26-27 20.4477500462839 29.80120303707135 29.546522403923497 20.204524512721253 28-29 20.39115773340762 29.851027685196335 29.44069257338772 20.31712200800832 30-31 20.39195802662963 29.65200704977583 29.619309721149236 20.3367252024453 32-33 20.421525894535257 29.743595007220446 29.475672723522273 20.35920637472202 34-35 20.53848864878021 29.720202514233264 29.33182644508758 20.409482391898955 36-37 20.463086367291048 29.788291881710705 29.398460506252714 20.35016124474554 38-39 20.453297236805785 29.645110964472554 29.44573506461307 20.455856734108593 40-41 20.53296704169803 29.752980908730244 29.388835582444482 20.32521646712724 42-43 20.548480558779826 29.717826028700678 29.367532490708133 20.366160921811368 44-45 20.529062933706186 29.65545632347756 29.379609587279266 20.43587115553699 46-47 20.58065010434393 29.697864157803362 29.305072861955967 20.416412875896743 48-49 20.516735845237804 29.609185667372756 29.365117946196484 20.508960541192952 50-51 20.523126620416107 29.726032146512487 29.327229772201907 20.423611460869495 52-53 20.51894390824878 29.710057048199406 29.359688250003085 20.41131079354873 54-55 20.535730453845485 29.67211947404075 29.33292473224286 20.45922533987091 56-57 20.587321611321553 29.70302045069823 29.298305225819416 20.411352712160802 58-59 20.53484658181652 29.75230559294574 29.28187789709531 20.430969928142435 60-61 20.508579440806812 29.691445508513226 29.250250420192103 20.549724630487855 62-63 20.470033985216826 29.6922612578824 29.335551717586444 20.502153039314326 64-65 20.537208493459197 29.711272549631484 29.262877092395712 20.488641864513607 66-67 20.52405557583327 29.696805970421025 29.241429769173404 20.5377086845723 68-69 20.450723674145287 29.688480149049116 29.288636034287997 20.572160142517593 70-71 20.592301774069263 29.682431013696448 29.19415953452889 20.5311076777054 72-73 20.540723303228667 29.72207330986144 29.231565759704036 20.505637627205854 74-75 20.565285687952098 29.691634964171147 29.23824226745147 20.504837080425283 76-77 20.52996645185573 29.731498633980962 29.225298334207352 20.513236579955958 78-79 20.53842139507884 29.721141622388124 29.243379341211867 20.497057641321163 80-81 20.58279679370087 29.72859197265108 29.194640293862633 20.493970939785413 82-83 20.53985560076447 29.79896292758809 29.235216705463518 20.425964766183927 84-85 20.576970330620334 29.707517303073722 29.205635536172032 20.509876830133912 86-87 20.526719976922575 29.7457566142885 29.19927698946354 20.528246419325384 88-89 20.57408889943453 29.70414690214583 29.205968066887316 20.515796131532323 90-91 20.56141491081644 29.69843084625471 29.187633135955565 20.55252110697329 92-93 20.515580024961892 29.702858896095957 29.231481548625478 20.550079530316665 94-95 20.54798462219292 29.71411722225322 29.17890384201805 20.558994313535813 96-97 20.620168679464747 29.72252450241101 29.128369119801334 20.52893769832291 98-99 20.59571064915104 29.73603515910858 29.133968140269808 20.53428605147057 100-101 20.720264790697225 29.659162841881244 28.87968744556875 20.740884921852782 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 522.0 1 545.0 2 615.5 3 743.5 4 919.0 5 1097.5 6 1359.5 7 1619.0 8 1884.5 9 2327.5 10 2753.5 11 3307.0 12 4114.5 13 5139.0 14 6466.0 15 8368.0 16 11457.5 17 16415.0 18 23727.5 19 34182.5 20 48480.5 21 66636.5 22 88030.0 23 113694.5 24 143445.5 25 177327.5 26 215097.0 27 254785.0 28 295770.5 29 337168.5 30 377713.5 31 416257.5 32 453482.5 33 488869.0 34 521883.0 35 552502.0 36 580659.0 37 602708.5 38 613739.5 39 612043.5 40 599736.5 41 579013.0 42 549429.5 43 516336.5 44 483872.5 45 455454.0 46 432090.5 47 413564.0 48 396097.5 49 374194.0 50 349199.0 51 322740.5 52 296490.0 53 272156.0 54 250059.0 55 229547.0 56 210935.5 57 192694.0 58 173282.5 59 152156.0 60 130641.5 61 110222.0 62 91566.0 63 75746.5 64 62976.5 65 52154.0 66 42919.0 67 35389.5 68 28741.0 69 23369.0 70 19004.5 71 15277.5 72 12414.5 73 10256.0 74 8719.0 75 7532.5 76 6598.5 77 5817.0 78 5160.5 79 4510.5 80 3893.0 81 3410.5 82 3004.5 83 2610.0 84 2200.0 85 1791.0 86 1380.0 87 1058.5 88 809.0 89 596.0 90 421.0 91 292.0 92 191.0 93 109.0 94 77.0 95 60.0 96 39.5 97 28.0 98 25.5 99 24.5 100 39.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02768116435105541 2 0.027806927651550406 3 0.0291108945040511 4 0.03809966092890363 5 0.021796765712105565 6 0.01018682734009428 7 0.010623689331287408 8 0.019241784975733638 9 0.007757609904217346 10-11 0.012093134210755198 12-13 0.01390015426523586 14-15 0.035203795456979485 16-17 0.025887382538732115 18-19 0.027965786557438813 20-21 0.013840582175527706 22-23 0.03736824804970907 24-25 0.04618491732651581 26-27 0.03509457995918121 28-29 0.036918147816358576 30-31 0.047154618564542984 32-33 0.04327912317297365 34-35 0.027899595346651974 36-37 0.03775215707227273 38-39 0.04715792812508233 40-41 0.028448982396182727 42-43 0.03771244234580062 44-45 0.054528319446196676 46-47 0.044506970133069486 48-49 0.057308350299243846 50-51 0.053194566548841894 52-53 0.036514381430558865 54-55 0.02767123566943739 56-57 0.03736824804970907 58-59 0.02817097931087801 60-61 0.0339560911336476 62-63 0.030193120800415894 64-65 0.031659256119344344 66-67 0.03282091186865334 68-69 0.03713657881195514 70-71 0.03838428313528702 72-73 0.031219084567611872 74-75 0.03199683129435721 76-77 0.03982394196990074 78-79 0.0340984022368393 80-81 0.03788784905438575 82-83 0.03974120295641719 84-85 0.04750874154225256 86-87 0.04815079628688489 88-89 0.04795553221506372 90-91 0.04862075388347143 92-93 0.049838672161949246 94-95 0.049070854116821924 96-97 0.054015337562598686 98-99 0.05250286839611945 100-101 0.055759475966831853 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5107746E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.37404166999877 #Duplication Level Percentage of deduplicated Percentage of total 1 98.20853817510819 93.66545212262433 2 1.179601084573021 2.250066459880861 3 0.20102231257183478 0.5751693124747705 4 0.13113623535438507 0.5002797110054356 5 0.04197470996526571 0.20016488686566822 6 0.03936171241797285 0.22524513602125507 7 0.035622408836716246 0.23782171733450955 8 0.026255041058031357 0.20032395039329692 9 0.02626199262052688 0.2254241140674598 >10 0.10957973056580782 1.859451254951393 >50 6.371085782527666E-4 0.03814066680829731 >100 0.0 0.0 >500 0.0 0.0 >1k 9.488349990540208E-6 0.022460667572704936 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.309560539341871E-5 6.619121078683743E-6 6.619121078683743E-6 2 3.309560539341871E-5 1.3238242157367486E-5 2.6476484314734972E-5 3 3.971472647210246E-5 1.3238242157367486E-5 3.309560539341871E-5 4 4.63338475507862E-5 1.3238242157367486E-5 3.309560539341871E-5 5 5.9572089708153684E-5 1.3238242157367486E-5 4.63338475507862E-5 6 5.9572089708153684E-5 1.3238242157367486E-5 7.281033186552116E-5 7 5.9572089708153684E-5 1.985736323605123E-5 9.26676951015724E-5 8 7.942945294420492E-5 1.985736323605123E-5 9.928681618025614E-5 9 9.26676951015724E-5 1.985736323605123E-5 9.928681618025614E-5 10-11 1.2907286103433299E-4 1.985736323605123E-5 1.1252505833762363E-4 12-13 1.588589058884098E-4 2.3166923775393103E-5 1.2245373995564923E-4 14-15 1.9857363236051227E-4 2.6476484314734972E-5 1.2576330049499112E-4 16-17 2.4159791937195663E-4 2.6476484314734972E-5 1.4231110319170046E-4 18-19 2.9455088800142655E-4 3.309560539341871E-5 1.654780269670936E-4 20-21 3.4088473555221274E-4 3.309560539341871E-5 1.720971480457773E-4 22-23 4.037663857997083E-4 3.971472647210246E-5 1.8864495074248666E-4 24-25 4.6995759658654575E-4 3.971472647210246E-5 2.217405561359054E-4 26-27 5.493870495307507E-4 3.971472647210246E-5 2.5152660098998224E-4 28-29 6.42054744632323E-4 3.971472647210246E-5 2.647648431473497E-4 30-31 7.512702424306047E-4 4.63338475507862E-5 2.680744036866916E-4 32-33 8.50557058610861E-4 4.63338475507862E-5 2.7469352476537537E-4 34-35 9.730107985665103E-4 5.2952968629469945E-5 2.8131264584405904E-4 36-37 0.0010954645385221596 5.6262529168811815E-5 3.243369328555034E-4 38-39 0.0011715844309270226 6.619121078683742E-5 3.5412297770958024E-4 40-41 0.0012973477314220136 6.619121078683742E-5 3.772899014849733E-4 42-43 0.0014330397135350302 6.950077132617929E-5 4.0045682526036643E-4 44-45 0.0016084464221201495 7.281033186552116E-5 4.170046279570758E-4 46-47 0.0017639957674692175 7.281033186552116E-5 4.302428701144433E-4 48-49 0.001985736323605123 7.281033186552116E-5 4.7988627820457137E-4 50-51 0.002171071713808268 7.611989240486304E-5 5.1298188359799E-4 52-53 0.002363026225090096 7.942945294420492E-5 5.229105652160157E-4 54-55 0.0026377197498554713 7.942945294420492E-5 5.295296862946994E-4 56-57 0.0030083905302617613 7.942945294420492E-5 5.526966100700925E-4 58-59 0.003494895929545016 7.942945294420492E-5 5.6593485222746E-4 60-61 0.004060830781772476 7.942945294420492E-5 5.857922154635112E-4 62-63 0.00488160179552926 7.942945294420492E-5 6.056495786995624E-4 64-65 0.006129306118861146 8.604857402288866E-5 6.155782603175881E-4 66-67 0.007383629563271715 9.26676951015724E-5 6.321260630142974E-4 68-69 0.009332960720944077 9.26676951015724E-5 6.519834262503487E-4 70-71 0.01211299157399125 9.26676951015724E-5 6.784599105650836E-4 72-73 0.01604143993419005 9.26676951015724E-5 6.95007713261793E-4 74-75 0.02170078845646465 9.597725564091427E-5 7.049363948798186E-4 76-77 0.02905463197488229 9.928681618025614E-5 7.214841975765279E-4 78-79 0.03892705106373909 9.928681618025614E-5 7.380320002732374E-4 80-81 0.052089173328701714 1.0590593725893989E-4 7.74437166205998E-4 82-83 0.0687892158102208 1.0590593725893989E-4 7.942945294420491E-4 84-85 0.09058267196178701 1.0921549779828175E-4 8.075327715994166E-4 86-87 0.11798252366699838 1.1252505833762363E-4 8.406283769928353E-4 88-89 0.15267333724037985 1.1914417941630737E-4 8.538666191502028E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCGTA 470 0.0 25.776314 46-47 TCCGATC 1095 0.0 18.003923 32-33 TCGCCGT 675 0.0 17.595798 44-45 CGATCTA 910 0.0 13.572149 34-35 GTCGCCG 965 0.0 13.292578 44-45 GCCGTAT 930 0.0 13.026739 46-47 CCGATCT 1320 0.0 12.775785 32-33 CTTCCGA 1940 0.0 10.897008 30-31 TTCCGAT 1615 0.0 10.442508 30-31 TGGTCGC 1290 0.0 10.127495 42-43 GTGGTCG 1435 0.0 9.434922 40-41 GACGTGT 1590 0.0 9.261283 20-21 TCCGACG 340 7.5299977E-6 9.081079 18-19 TCAGACG 1660 0.0 9.013748 18-19 CCGTATC 1360 0.0 8.908168 48-49 GGTCGCC 1490 0.0 8.7681 42-43 GAGCGTC 3260 0.0 8.30472 9 AGATCGG 5870 0.0 8.21321 94-95 AGCGTCG 2085 0.0 8.201276 94-95 GACCGGA 815 8.2957595E-6 8.159023 9 >>END_MODULE