FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n02 60355-bar2.342000000043bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n02 60355-bar2.342000000043bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15805611
Sequences flagged as poor quality0
Sequence length101
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT8500.015.92755432-33
TCCGATC10900.014.59961732-33
GACCGGA5001.0106305E-813.2991579
GTCAGTG30600.011.1751263
GTGACTG21300.08.9179587
CAGTGAC30800.08.7887495
GTCAGAG20250.08.6779693
TGACTGG26200.08.5194288
TGGTCAG57300.08.2057921
GACGGGA9356.282302E-67.6198389
CGATCTA16550.07.606065834-35
GACTGGA48800.07.39707239
GGTCAGT51850.07.0531312
CAGAGAC29650.06.7270945
CAGACGT15450.06.60991718-19
CGTGTGC18100.06.29820322-23
GGTCAGA38100.05.8588572
AGTGACT35000.05.83392336
TGACGGG12352.9957524E-45.76817238
GTCAGCG11557.9724676E-45.7568843
Produced by FastQC (version 0.11.2)