##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l01n02 50617-bar10.342000000044ba.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14184117
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.91754918547274	38.0	38.0	38.0	32.0	38.0
2	35.90481360242587	38.0	38.0	38.0	32.0	38.0
3	35.92938432473449	38.0	38.0	38.0	32.0	38.0
4	35.96777896008613	38.0	38.0	38.0	32.0	38.0
5	35.79483382716034	38.0	32.0	38.0	32.0	38.0
6	38.04377121254711	40.0	38.0	40.0	38.0	40.0
7	38.1546161809015	40.0	38.0	40.0	38.0	40.0
8	38.23506052579798	40.0	38.0	40.0	38.0	40.0
9	38.243183343735815	40.0	38.0	40.0	38.0	40.0
10-11	38.21569234799741	40.0	38.0	40.0	38.0	40.0
12-13	38.23117875437717	40.0	38.0	40.0	38.0	40.0
14-15	38.17256745696612	40.0	38.0	40.0	38.0	40.0
16-17	38.18857194282873	40.0	38.0	40.0	38.0	40.0
18-19	38.17442858797625	40.0	38.0	40.0	38.0	40.0
20-21	38.15840302219729	40.0	38.0	40.0	38.0	40.0
22-23	38.16162648686556	40.0	38.0	40.0	38.0	40.0
24-25	38.105925945196304	40.0	38.0	40.0	38.0	40.0
26-27	38.10767656527368	40.0	38.0	40.0	38.0	40.0
28-29	38.00846725249094	40.0	38.0	40.0	38.0	40.0
30-31	37.99771371034235	40.0	38.0	40.0	38.0	40.0
32-33	37.99560550720217	40.0	38.0	40.0	38.0	40.0
34-35	37.944947930139044	40.0	38.0	40.0	38.0	40.0
36-37	37.95491746155224	40.0	38.0	40.0	38.0	40.0
38-39	37.93279313756366	40.0	38.0	40.0	38.0	40.0
40-41	37.85378402476516	40.0	38.0	40.0	35.0	40.0
42-43	37.68636123066385	40.0	38.0	40.0	32.0	40.0
44-45	37.57818022792677	40.0	38.0	40.0	32.0	40.0
46-47	37.52515426233441	40.0	38.0	40.0	32.0	40.0
48-49	37.49773577727821	40.0	38.0	40.0	32.0	40.0
50-51	37.420126645881446	40.0	38.0	40.0	32.0	40.0
52-53	37.38404643024307	40.0	38.0	40.0	32.0	40.0
54-55	37.37316968691107	40.0	38.0	40.0	32.0	40.0
56-57	37.3139696323712	40.0	38.0	40.0	32.0	40.0
58-59	37.265133141527244	40.0	38.0	40.0	32.0	40.0
60-61	37.23533065188337	39.0	38.0	40.0	32.0	40.0
62-63	37.20869913862104	38.0	38.0	40.0	32.0	40.0
64-65	37.138405689969986	38.0	38.0	40.0	32.0	40.0
66-67	37.12160295209071	38.0	38.0	40.0	32.0	40.0
68-69	37.027903146879005	38.0	38.0	40.0	32.0	40.0
70-71	36.91522739836395	38.0	38.0	40.0	32.0	40.0
72-73	36.88087464309551	38.0	38.0	40.0	32.0	40.0
74-75	36.77710498298907	38.0	38.0	40.0	32.0	40.0
76-77	36.71534417687051	38.0	38.0	40.0	32.0	40.0
78-79	36.686928202862404	38.0	38.0	40.0	32.0	40.0
80-81	36.604650363501655	38.0	38.0	40.0	32.0	40.0
82-83	36.52176557765281	38.0	38.0	40.0	32.0	40.0
84-85	36.39663311434896	38.0	38.0	40.0	32.0	40.0
86-87	36.32266273607303	38.0	38.0	40.0	27.0	40.0
88-89	36.287322996560164	38.0	38.0	40.0	27.0	40.0
90-91	36.18465830477851	38.0	38.0	40.0	27.0	40.0
92-93	36.13221182538187	38.0	38.0	40.0	27.0	40.0
94-95	36.044379851068626	38.0	38.0	40.0	27.0	40.0
96-97	35.934399617544045	38.0	38.0	40.0	27.0	40.0
98-99	35.834953525834564	38.0	38.0	40.0	27.0	40.0
100-101	33.88152748599014	38.0	32.5	39.0	20.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.03837208776497647
2201	2	-0.0525762854725329
2201	3	-0.0855188368781512
2201	4	0.02514747759989433
2201	5	0.08549026461397347
2201	6	0.06731845750336873
2201	7	0.11116534262482247
2201	8	0.15240198558193185
2201	9	0.10950722966815363
2201	10-11	0.1918185296657171
2201	12-13	0.17057516247438542
2201	14-15	0.17068807874351677
2201	16-17	0.08619052492841206
2201	18-19	0.10430141745151644
2201	20-21	0.14182877963485652
2201	22-23	0.06200163753085519
2201	24-25	0.0911950365982861
2201	26-27	0.09429097419955923
2201	28-29	0.059196889478414505
2201	30-31	0.08406416089795243
2201	32-33	0.1245899349077888
2201	34-35	0.13925012415464977
2201	36-37	0.1439192380860419
2201	38-39	0.1235764007907747
2201	40-41	0.07472008053425583
2201	42-43	0.05658513924298347
2201	44-45	0.052768955017370445
2201	46-47	0.05933283888484908
2201	48-49	0.03593198189564362
2201	50-51	0.090025607655285
2201	52-53	0.01411165824053029
2201	54-55	0.06337996715122785
2201	56-57	0.04185648903846584
2201	58-59	0.03720411140565005
2201	60-61	-0.032986602978674284
2201	62-63	0.051910146827623294
2201	64-65	0.02198629872283675
2201	66-67	-0.03698297196238087
2201	68-69	-0.05916467429133121
2201	70-71	-0.06310439718046013
2201	72-73	-0.017168712226272476
2201	74-75	0.015795081831910807
2201	76-77	0.03242779496884651
2201	78-79	-0.013744794665932147
2201	80-81	-0.04557743639233536
2201	82-83	-0.09911449486327939
2201	84-85	-0.08526160293909157
2201	86-87	0.015215451966412274
2201	88-89	-0.09484158484089988
2201	90-91	-0.08445317689142229
2201	92-93	-0.07171154585016382
2201	94-95	-0.06877538336143374
2201	96-97	-0.09914751013677403
2201	98-99	-0.07655204845692509
2201	100-101	-0.01387983326956288
1101	1	0.07739015762273738
1101	2	0.06639096728945049
1101	3	0.13611458325003412
1101	4	0.06539645445248965
1101	5	0.0455303344475908
1101	6	0.07294775021669153
1101	7	0.14456612129490765
1101	8	0.14623044312781985
1101	9	0.19375565042693665
1101	10-11	0.21494250459321051
1101	12-13	0.20480094266720528
1101	14-15	0.18881677172742428
1101	16-17	0.1250493629837166
1101	18-19	0.14236545897984598
1101	20-21	0.18169621341869657
1101	22-23	0.05991985427896651
1101	24-25	0.09455754774478464
1101	26-27	0.08942881068106345
1101	28-29	0.18064185374231556
1101	30-31	0.17191838939617554
1101	32-33	0.13644096558910235
1101	34-35	0.1135908359519604
1101	36-37	0.12513913558884582
1101	38-39	0.08996270673089413
1101	40-41	0.05658476381815092
1101	42-43	0.027079486940642994
1101	44-45	0.037540396714170754
1101	46-47	-0.0031470842276846156
1101	48-49	0.07276667593374242
1101	50-51	-0.012674664855659046
1101	52-53	-0.007014810506873914
1101	54-55	0.022900698364644256
1101	56-57	0.002639396424015672
1101	58-59	0.03771577598674014
1101	60-61	-0.01813755234097414
1101	62-63	0.09504654506849164
1101	64-65	0.03932791916744094
1101	66-67	-0.04513671552960119
1101	68-69	-0.01068177258049019
1101	70-71	-0.034612059356803115
1101	72-73	-0.04868737695733927
1101	74-75	0.0029243432580230433
1101	76-77	0.03915041418180465
1101	78-79	0.05134128402217186
1101	80-81	0.03142424118507847
1101	82-83	0.027078789082963794
1101	84-85	0.04402031733831535
1101	86-87	0.1256190154066843
1101	88-89	0.03240914867431144
1101	90-91	0.0540449466388182
1101	92-93	0.025270408988859572
1101	94-95	-0.0055619603001630935
1101	96-97	0.016904262641176615
1101	98-99	0.002721470752526045
1101	100-101	0.2361127257845368
2202	1	0.015802576885320718
2202	2	-0.036244665605828175
2202	3	0.08666969175165917
2202	4	0.05556069646365103
2202	5	0.0046861631884809185
2202	6	0.03531347820482722
2202	7	0.13024612522064416
2202	8	0.13773346559140265
2202	9	0.14780461842343584
2202	10-11	0.1512233248211814
2202	12-13	0.1355163781809381
2202	14-15	0.1528332611659522
2202	16-17	0.17310888330690943
2202	18-19	0.14140406598240673
2202	20-21	0.15922935810177563
2202	22-23	0.13796644199016583
2202	24-25	0.109766793364372
2202	26-27	0.09501201377861435
2202	28-29	0.08934333919525272
2202	30-31	0.09469917126059357
2202	32-33	0.16852170146796652
2202	34-35	0.12442223365509619
2202	36-37	0.12155409715230547
2202	38-39	0.1256975882344591
2202	40-41	0.11049682400710026
2202	42-43	0.05474209336777136
2202	44-45	0.04134728657106024
2202	46-47	0.03129177288136731
2202	48-49	-0.02422718842826299
2202	50-51	0.07395793119312799
2202	52-53	0.0971501592870041
2202	54-55	0.03827971730112978
2202	56-57	0.013800345145945414
2202	58-59	-0.01705374855001196
2202	60-61	-0.05860754795322265
2202	62-63	0.003860146030966405
2202	64-65	-0.0013045445236343767
2202	66-67	-0.0605034125657582
2202	68-69	-0.04726177442596935
2202	70-71	0.03316019471492382
2202	72-73	0.010792050944786524
2202	74-75	0.008282894030486432
2202	76-77	-0.0016629172176436668
2202	78-79	-0.006193717840190516
2202	80-81	0.0038276120585862827
2202	82-83	-0.015459367802236557
2202	84-85	0.05187765223335106
2202	86-87	0.04703571895344538
2202	88-89	0.0085990254758741
2202	90-91	0.040647895488028496
2202	92-93	0.04080168713549881
2202	94-95	0.010922127384219493
2202	96-97	0.024634602382789694
2202	98-99	0.0336086389161423
2202	100-101	0.2516769789280602
1102	1	-0.10652996212203902
1102	2	-0.06987830190702482
1102	3	-0.05651240105732569
1102	4	0.012780254464821894
1102	5	-0.18110302664027955
1102	6	-0.14641303839531616
1102	7	0.006859732751024694
1102	8	0.0662313111537074
1102	9	0.0395386874861785
1102	10-11	0.07945824603382334
1102	12-13	0.1059301286172456
1102	14-15	0.0990951812871188
1102	16-17	0.09555464558591353
1102	18-19	0.05205259891275915
1102	20-21	0.09555326566098898
1102	22-23	0.06439730613330852
1102	24-25	-0.001690351444167959
1102	26-27	-0.011339517825831535
1102	28-29	0.05549444530636549
1102	30-31	0.09132554172376217
1102	32-33	0.10989239466936596
1102	34-35	0.03378761195165225
1102	36-37	0.08424363550088287
1102	38-39	0.06151129504319641
1102	40-41	0.036828828828134874
1102	42-43	-0.05590868110778047
1102	44-45	-0.13950547256158075
1102	46-47	-0.10022298960765141
1102	48-49	-0.09868389197428229
1102	50-51	-0.06584066238070108
1102	52-53	-0.0955777150903927
1102	54-55	-0.05290163648026436
1102	56-57	-0.1385680169346699
1102	58-59	-0.1442286415878229
1102	60-61	-0.16007861012711544
1102	62-63	-0.10823102226213877
1102	64-65	-0.22723363508123384
1102	66-67	-0.19615344421398362
1102	68-69	-0.18849869580107992
1102	70-71	-0.1478139787018975
1102	72-73	-0.10282413666322299
1102	74-75	-0.12156641837824367
1102	76-77	-0.06071974606800268
1102	78-79	-0.13999099708777862
1102	80-81	-0.0833417309163309
1102	82-83	-0.10606494859689519
1102	84-85	-0.145698797052205
1102	86-87	-0.05303409083563082
1102	88-89	-0.056614855249577545
1102	90-91	-0.0912152247377307
1102	92-93	-0.17917339820492373
1102	94-95	-0.2568308447152532
1102	96-97	-0.1720473861513483
1102	98-99	-0.13172501773879475
1102	100-101	0.17836599998370417
2203	1	-0.10600146761093043
2203	2	-0.0329865371971394
2203	3	-0.02654802167813841
2203	4	-0.013313850007584449
2203	5	-0.06215602188689218
2203	6	-0.10002363279279791
2203	7	0.0034173704781323977
2203	8	0.038955922739170035
2203	9	0.07255127071583445
2203	10-11	0.1177456481111534
2203	12-13	0.09104789677743241
2203	14-15	0.09055468502646846
2203	16-17	0.11340222745121764
2203	18-19	0.03966009473035825
2203	20-21	0.0802837113357171
2203	22-23	0.06641538250632806
2203	24-25	0.08752875196455534
2203	26-27	0.07463427415632395
2203	28-29	0.06677603882283734
2203	30-31	0.054152425733512644
2203	32-33	0.10532267009806162
2203	34-35	0.055108417083197025
2203	36-37	0.05254749488367594
2203	38-39	0.07708310556804321
2203	40-41	0.022584920328711178
2203	42-43	-0.044403855957440896
2203	44-45	-0.10980480150516314
2203	46-47	-0.07927160872002759
2203	48-49	-0.07444289371191815
2203	50-51	-0.12814404409885327
2203	52-53	-0.15016740853975108
2203	54-55	-0.06261536105611043
2203	56-57	-0.08400192306847032
2203	58-59	-0.12039153090294263
2203	60-61	-0.1337421277244104
2203	62-63	-0.11346910858556214
2203	64-65	-0.1366117463543972
2203	66-67	-0.07279348760091153
2203	68-69	-0.10461701253812095
2203	70-71	-0.11223020386915294
2203	72-73	-0.05213285608517282
2203	74-75	-0.04226382009817797
2203	76-77	-0.07083321694832989
2203	78-79	-0.10101268317588108
2203	80-81	-0.06372406685525789
2203	82-83	-0.13200616634674134
2203	84-85	-0.18571730797523855
2203	86-87	-0.018423203145253808
2203	88-89	-0.051658091841638054
2203	90-91	-0.07989533862841114
2203	92-93	-0.17725269847728242
2203	94-95	-0.09974779290338631
2203	96-97	-0.13395391478314878
2203	98-99	-0.1655233933386384
2203	100-101	-0.0699444173601762
1103	1	-0.13898484400522904
1103	2	-0.14438208549201192
1103	3	-0.13393529516412173
1103	4	-0.057197860626331476
1103	5	-0.2594655370292571
1103	6	-0.2076924865423564
1103	7	-0.10022555790452259
1103	8	-0.04283836125014062
1103	9	-0.03070983265120475
1103	10-11	0.06352716474511766
1103	12-13	0.02970334095650884
1103	14-15	0.029751697098333807
1103	16-17	0.012463054399404427
1103	18-19	-0.013420922041227357
1103	20-21	0.017115240843573076
1103	22-23	-0.01825562873095521
1103	24-25	0.006582884485794693
1103	26-27	-0.03399655868129514
1103	28-29	-0.012347097791419515
1103	30-31	-0.019398574139223967
1103	32-33	0.04465488473405799
1103	34-35	0.01130292836339919
1103	36-37	-0.015162176521180015
1103	38-39	-0.060509138130413476
1103	40-41	-0.05061041665666721
1103	42-43	-0.14040937249669128
1103	44-45	-0.13479211405677205
1103	46-47	-0.11414593875941392
1103	48-49	-0.08580555585644589
1103	50-51	-0.08032603070407873
1103	52-53	-0.18984411092778686
1103	54-55	-0.1365604738580899
1103	56-57	-0.1985333398187379
1103	58-59	-0.11401954332107778
1103	60-61	-0.20100284043523686
1103	62-63	-0.09355642024930688
1103	64-65	-0.13343629732171536
1103	66-67	-0.17704012780949796
1103	68-69	-0.15128885835913053
1103	70-71	-0.2241812652998405
1103	72-73	-0.2208079159413998
1103	74-75	-0.22743799061961312
1103	76-77	-0.2043275065607233
1103	78-79	-0.283794611182671
1103	80-81	-0.2526624881898272
1103	82-83	-0.21455358816370307
1103	84-85	-0.254358540554783
1103	86-87	-0.175451907498406
1103	88-89	-0.24166086194933456
1103	90-91	-0.22793708557196624
1103	92-93	-0.34857096195122494
1103	94-95	-0.34128095280903636
1103	96-97	-0.35938788944376654
1103	98-99	-0.29363080012126375
1103	100-101	-0.030373192149824035
2204	1	-0.21959665265516293
2204	2	-0.14026897706278874
2204	3	-0.053455798970986734
2204	4	-0.004769782686935287
2204	5	-0.07950019179547496
2204	6	-0.15455604749777763
2204	7	-0.08484805801447948
2204	8	-0.045796255167850575
2204	9	0.04157288654345592
2204	10-11	0.050811385029795986
2204	12-13	0.024582670687678387
2204	14-15	0.029824543383782043
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1213	6	0.05081505703370226
1213	7	-0.012151551771445668
1213	8	-0.09984346680860767
1213	9	-0.1624264514708713
1213	10-11	-0.31738835912397434
1213	12-13	-0.1681684818586433
1213	14-15	-0.15954185872430315
1213	16-17	-0.13278601838884185
1213	18-19	-0.07635595360957126
1213	20-21	-0.1027494898802459
1213	22-23	-0.07852898739488268
1213	24-25	-0.18254875600958087
1213	26-27	-0.15225666193117604
1213	28-29	-0.051385197765711155
1213	30-31	-0.2534476458599144
1213	32-33	-0.15779620145372064
1213	34-35	-0.15957911357531174
1213	36-37	-0.1957877946994202
1213	38-39	-0.23632490388855842
1213	40-41	-0.1637440089895179
1213	42-43	-0.09093417479975585
1213	44-45	-0.001755215076364891
1213	46-47	-0.21194813239699073
1213	48-49	-0.22128979926143444
1213	50-51	-0.33367558130995434
1213	52-53	-0.2601052425842951
1213	54-55	-0.23108377447397999
1213	56-57	-0.20451156535471426
1213	58-59	-0.10241327181994109
1213	60-61	-0.07370532400163654
1213	62-63	-0.11879772127264943
1213	64-65	-0.10083704941077798
1213	66-67	-0.23709895871756004
1213	68-69	-0.12343765883313296
1213	70-71	-0.17136027299421386
1213	72-73	-0.11766093297149638
1213	74-75	-0.10746470784071249
1213	76-77	-0.12697150083509712
1213	78-79	-0.102261029169469
1213	80-81	-0.10226419413491783
1213	82-83	-0.13566184041512486
1213	84-85	-0.024387883949181344
1213	86-87	-0.15745932875100976
1213	88-89	-0.13942919467623938
1213	90-91	-0.1344808475652215
1213	92-93	-0.2107535450488811
1213	94-95	-0.2992059894393435
1213	96-97	-0.28969070359485727
1213	98-99	-0.3438159595732202
1213	100-101	-1.196938251768053
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	21.0
4	53.0
5	90.0
6	106.0
7	82.0
8	87.0
9	114.0
10	150.0
11	257.0
12	309.0
13	359.0
14	834.0
15	4810.0
16	19727.0
17	37422.0
18	42891.0
19	42768.0
20	45293.0
21	52057.0
22	60744.0
23	70588.0
24	79550.0
25	88638.0
26	99842.0
27	112452.0
28	129638.0
29	149334.0
30	173731.0
31	191619.0
32	223578.0
33	272043.0
34	343186.0
35	458388.0
36	676141.0
37	1185245.0
38	3104572.0
39	6517398.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.39479501235698	32.30381421477556	29.1571972134473	20.144193559420167
2	20.949985243420098	33.53611730229268	28.288219101185003	17.225678353102214
3	20.625071579326253	31.00648572165219	29.311473171645392	19.056969527376168
4	20.17033883476185	30.293558335927017	29.043752377248634	20.492350452062503
5	20.505338861755984	30.26482088487435	29.9035986059958	19.32624164737386
6	20.662403916431746	30.140651579465867	28.848394657549836	20.348549846552554
7	20.51329056946046	30.654125649524268	29.331795213996102	19.50078856701917
8	19.67131121531573	30.764446638225856	29.819363254944825	19.744878891513594
9	20.241118970263074	30.01647037588556	30.038828065069268	19.703582588782094
10-11	20.069412595103078	30.607944380235548	29.597550576714916	19.72509244794646
12-13	19.95015565340939	30.279640252006924	29.866388382579512	19.903815712004175
14-15	19.79659719026009	30.27347422326908	30.019318566346847	19.910610020123976
16-17	20.106253091617514	30.040115351513275	30.089722270714148	19.763909286155055
18-19	19.996217243881354	29.933723489413396	30.108058899571986	19.962000367133264
20-21	20.20329341738529	30.172953047432248	29.688887021140744	19.934866514041715
22-23	20.404448467661084	29.871445533353807	29.64790075898444	20.076205240000665
24-25	20.102276004708582	30.249266461924684	29.60039764362157	20.048059889745165
26-27	20.441574444544436	30.014986065476883	29.529725007920067	20.013714482058614
28-29	20.393245072332338	30.06303348001027	29.448729550017426	20.094991897639968
30-31	20.402214715257497	29.691275938463267	29.770003610688118	20.136505735591122
32-33	20.221922156170308	30.000653477702183	29.658531627492035	20.118892738635473
34-35	20.510746383942642	29.968931654187813	29.353421500708542	20.166900461161006
36-37	20.24473771006834	29.78724867344052	29.63017844491199	20.33783517157915
38-39	20.24381234359327	29.670332576987608	29.667331317022704	20.418523762396422
40-41	20.763133541758457	29.763040277599274	29.37103814645153	20.10278803419075
42-43	20.528722617716568	29.728317165581508	29.60395843382123	20.13900178288069
44-45	20.523560050616698	29.64373619752166	29.604755780208293	20.22794797165335
46-47	20.54755442339037	29.720867919067977	29.329374219132998	20.40220343840865
48-49	20.29333391553962	29.649557079395077	29.360878227616393	20.696230777448907
50-51	20.532936035881484	29.728603384994607	29.513919847905484	20.224540731218426
52-53	20.322373249842354	29.940386222182948	29.550642618695605	20.186597909279094
54-55	20.306717556008373	29.889797939440754	29.35406776761888	20.449416736931997
56-57	20.33578335520286	29.757200634610086	29.711999300363185	20.195016709823868
58-59	20.31080903634756	30.17150942084268	29.31997424005409	20.197707302755667
60-61	20.29010528349884	30.118432916635374	29.269119461526227	20.322342338339563
62-63	20.25569229335999	30.092330370135663	29.366743217536406	20.285234118967942
64-65	20.32886533627364	30.118928161675136	29.284784661615948	20.26742184043528
66-67	20.293848223894006	30.11081255085235	29.267981430415436	20.327357794838207
68-69	20.3004721046585	30.004689697303828	29.32018572456705	20.37465247347063
70-71	20.47378880928342	29.95454075320567	29.257691275676155	20.313979161834755
72-73	20.402837187133468	29.963782502912515	29.32149325497557	20.311887054978445
74-75	20.42652928467275	29.95142470511937	29.31614394050736	20.305902069700522
76-77	20.389754140909407	29.969461568930143	29.286788445175695	20.353995844984755
78-79	20.389615990413645	29.950853427708328	29.328150809483432	20.331379772394595
80-81	20.491695376573343	29.91530958087762	29.26227990976784	20.330715132781197
82-83	20.446830973523166	29.98325771516548	29.318295322535132	20.251615988776223
84-85	20.485945256612276	29.893139936603415	29.2873033316358	20.333611475148512
86-87	20.43718943211941	29.93944463055892	29.272860513372894	20.350505423948775
88-89	20.448713578717527	29.905232446616438	29.26336814079138	20.38268583387466
90-91	20.48170293248309	29.878984943909042	29.25973381857902	20.379578305028843
92-93	20.418318037783166	29.91965639254494	29.28566601876772	20.376359550904176
94-95	20.457366967587767	29.922166894315154	29.226736589297452	20.393729548799627
96-97	20.484327479893754	29.959757529415626	29.219945028996797	20.335969961693824
98-99	20.472919547627452	29.992191801988135	29.197825188134402	20.33706346225001
100-101	20.627853713785242	29.95883471131745	28.893229556108718	20.52008201878859
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1142.0
1	1113.5
2	1268.5
3	1593.0
4	1886.0
5	2202.0
6	2731.0
7	3460.5
8	4313.0
9	5195.0
10	6091.5
11	7223.5
12	8930.0
13	10818.0
14	12583.0
15	15004.5
16	18063.5
17	22507.5
18	29222.5
19	38794.5
20	51733.5
21	68255.0
22	88158.5
23	111146.0
24	138032.5
25	169026.0
26	203044.0
27	238615.5
28	276375.5
29	315237.0
30	351582.5
31	386846.5
32	422680.0
33	456060.5
34	486307.5
35	514388.0
36	540257.5
37	560501.5
38	570589.5
39	569904.0
40	559240.5
41	541140.5
42	515399.5
43	485080.0
44	455517.5
45	429105.0
46	407683.0
47	390170.0
48	372600.5
49	350745.0
50	326381.5
51	301105.5
52	275269.5
53	251981.5
54	232004.5
55	213630.5
56	195579.0
57	177519.0
58	159548.5
59	140146.0
60	119779.0
61	100246.0
62	83164.0
63	68645.0
64	56599.5
65	46890.0
66	38425.5
67	31484.0
68	25644.0
69	20742.5
70	16690.5
71	13354.5
72	10823.0
73	9045.0
74	7642.5
75	6438.0
76	5544.0
77	4878.0
78	4368.0
79	3866.5
80	3309.0
81	2862.5
82	2497.5
83	2096.5
84	1726.5
85	1401.5
86	1065.0
87	790.0
88	555.0
89	391.5
90	298.0
91	203.0
92	118.0
93	87.0
94	65.0
95	50.0
96	47.0
97	32.0
98	21.5
99	20.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027298139179195997
2	0.0282146572818033
3	0.028517813269588795
4	0.03832455696748695
5	0.02184133139905713
6	0.009947746482914658
7	0.010074648989429514
8	0.018450214419410103
9	0.00759299997313897
10-11	0.011819558454008805
12-13	0.013867623906373586
14-15	0.03532824778588614
16-17	0.02563078124637579
18-19	0.028059554218285144
20-21	0.013095633658408204
22-23	0.037379838307876334
24-25	0.04651681877694608
26-27	0.03486293859533167
28-29	0.036826402376686544
30-31	0.04768714189258309
32-33	0.04319972825943272
34-35	0.027696472046867635
36-37	0.03826463078385493
38-39	0.04750383827206163
40-41	0.027950277059897347
42-43	0.03791564889093907
44-45	0.05476548170041181
46-47	0.04488471154037999
48-49	0.057504460799357476
50-51	0.05406751791458009
52-53	0.03651972131927564
54-55	0.027759923300125063
56-57	0.0375913424854011
58-59	0.027939701851021112
60-61	0.03371376589744712
62-63	0.02976216284735948
64-65	0.031179240836775386
66-67	0.03233193860428534
68-69	0.03640339402163702
70-71	0.03788392326431036
72-73	0.030798533317230814
74-75	0.031591673982948675
76-77	0.03940322827286323
78-79	0.033815992916584096
80-81	0.03777817117554797
82-83	0.039805086210160275
84-85	0.047214782562777786
86-87	0.04766951654445603
88-89	0.04754261403794117
90-91	0.04816302629201381
92-93	0.04941442600903532
94-95	0.04881163910308975
96-97	0.05324265162223352
98-99	0.0518220485631922
100-101	0.055364743536731965
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4184117E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.0466744779736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.4036790532382	93.52942450408122
2	1.0093527085969052	1.9187123665494201
3	0.18357065087781116	0.5234333969307917
4	0.11804312396426267	0.4487842551117744
5	0.05510239004701212	0.26186494648783465
6	0.037182772282792875	0.212045931201074
7	0.026254093621819685	0.17467550031311374
8	0.022978835125402646	0.1747249489637737
9	0.011675093108980093	0.09987108968063374
>10	0.1304648105491735	2.1670709583561587
>50	0.001318202481679678	0.07605058480759587
>100	3.7084345984035686E-4	0.06626480672005251
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	7.422646089623023E-6	0.3470767107965709
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	49196	0.346838650583607	Illumina Single End PCR Primer 1 (100% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.230083550495247E-5	0.0	7.050139250825413E-6
2	5.64011140066033E-5	0.0	2.1150417752476237E-5
3	8.460167100990495E-5	0.0	2.820055700330165E-5
4	9.870194951155578E-5	0.0	4.935097475577789E-5
5	1.128022280132066E-4	0.0	7.755153175907954E-5
6	1.4100278501650825E-4	0.0	9.870194951155578E-5
7	1.692033420198099E-4	0.0	1.1985236726403202E-4
8	2.0445403827393696E-4	7.050139250825413E-6	1.2690250651485743E-4
9	2.1855431677558778E-4	1.4100278501650826E-5	1.4100278501650825E-4
10-11	2.784805004076038E-4	1.762534812706353E-5	1.621532027689845E-4
12-13	3.348816144142071E-4	2.1150417752476237E-5	1.7977855089604803E-4
14-15	4.2653342467493746E-4	2.1150417752476237E-5	2.0797910789934967E-4
16-17	5.463857919389694E-4	2.4675487377888946E-5	2.573300826551276E-4
18-19	6.168871844472235E-4	3.172562662871436E-5	2.820055700330165E-4
20-21	7.437896909620811E-4	3.877576587953977E-5	3.0668105741090546E-4
22-23	8.565919189752877E-4	4.230083550495247E-5	3.3135654478879436E-4
24-25	9.693941469884942E-4	4.230083550495247E-5	3.454568232904452E-4
26-27	0.0010716211661254628	4.230083550495247E-5	3.6660724104292143E-4
28-29	0.0012267242296436218	4.230083550495247E-5	3.807075195445723E-4
30-31	0.001402977710914257	4.230083550495247E-5	3.9480779804622313E-4
32-33	0.0015263551478037019	4.230083550495247E-5	4.230083550495247E-4
34-35	0.0016849832809472736	4.935097475577789E-5	4.5473398167823916E-4
36-37	0.0019000125280974486	4.935097475577789E-5	4.5825905130365184E-4
38-39	0.0030315598778549273	4.935097475577789E-5	4.758843994307154E-4
40-41	0.0032606894035067534	4.935097475577789E-5	4.93509747557779E-4
42-43	0.0034369428847773886	4.935097475577789E-5	5.146601653102552E-4
44-45	0.0036942729674325162	4.935097475577789E-5	5.217103045610806E-4
46-47	0.003969228398214708	4.935097475577789E-5	5.252353741864932E-4
48-49	0.004282959594876438	5.64011140066033E-5	5.428607223135568E-4
50-51	0.004536764607906153	6.345125325742871E-5	5.781114185676838E-4
52-53	0.004896321709698249	6.345125325742871E-5	5.992618363201601E-4
54-55	0.005315804995122362	6.345125325742871E-5	6.345125325742871E-4
56-57	0.00584809050855968	6.345125325742871E-5	6.768133680792397E-4
58-59	0.006531954015889745	7.050139250825412E-5	7.191142035841921E-4
60-61	0.00747667267550035	7.050139250825412E-5	7.508398302129065E-4
62-63	0.008618795234134067	7.050139250825412E-5	7.719902479653827E-4
64-65	0.010060548710927864	7.050139250825412E-5	8.072409442195097E-4
66-67	0.012059263188536867	7.050139250825412E-5	8.495417797244622E-4
68-69	0.014597313318834017	7.050139250825412E-5	8.742172671023511E-4
70-71	0.017910878766721962	7.050139250825412E-5	8.918426152294146E-4
72-73	0.02278252498904232	7.755153175907954E-5	9.270933114835418E-4
74-75	0.029575334157212606	8.460167100990495E-5	9.517687988614308E-4
76-77	0.0385607366323896	8.460167100990495E-5	9.799693558647325E-4
78-79	0.05093373101758819	9.517687988614307E-5	9.940696343663832E-4
80-81	0.06797391758683322	9.870194951155578E-5	0.0010293203306205104
82-83	0.09072471694924683	9.870194951155578E-5	0.0010504707483729866
84-85	0.12030005110645943	9.870194951155578E-5	0.0010857214446271136
86-87	0.1578843434526097	9.870194951155578E-5	0.0011244972105066533
88-89	0.20698151319535787	9.870194951155578E-5	0.0011491726978845422
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGTC	8720	0.0	62.308815	9
AGAGCGT	9645	0.0	56.82133	8
AAGAGCG	10755	0.0	52.322277	7
CGGAAGA	11875	0.0	47.794266	4
TCGGAAG	12030	0.0	47.33571	3
ATCGGAA	12160	0.0	46.55626	2
GATCGGA	12435	0.0	45.56518	1
CGCCGTA	5495	0.0	44.828274	46-47
TCGCCGT	5680	0.0	43.11727	44-45
GTCGCCG	6175	0.0	41.7382	44-45
GCCGTAT	5945	0.0	41.19531	46-47
CCGTATC	6190	0.0	39.45023	48-49
TGGTCGC	6495	0.0	39.24169	42-43
GTGGTCG	6730	0.0	38.859352	40-41
GGTCGCC	6640	0.0	38.670944	42-43
CGTATCA	6365	0.0	38.4029	48-49
TCGGTGG	7140	0.0	37.82618	38-39
TCTCGGT	7110	0.0	37.4826	36-37
CTCGGTG	7480	0.0	35.723778	36-37
CGTCGTG	7495	0.0	35.61337	12-13
>>END_MODULE