FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n02 50617-bar10.342000000044ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n02 50617-bar10.342000000044ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14184117
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG491960.346838650583607Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC87200.062.3088159
AGAGCGT96450.056.821338
AAGAGCG107550.052.3222777
CGGAAGA118750.047.7942664
TCGGAAG120300.047.335713
ATCGGAA121600.046.556262
GATCGGA124350.045.565181
CGCCGTA54950.044.82827446-47
TCGCCGT56800.043.1172744-45
GTCGCCG61750.041.738244-45
GCCGTAT59450.041.1953146-47
CCGTATC61900.039.4502348-49
TGGTCGC64950.039.2416942-43
GTGGTCG67300.038.85935240-41
GGTCGCC66400.038.67094442-43
CGTATCA63650.038.402948-49
TCGGTGG71400.037.8261838-39
TCTCGGT71100.037.482636-37
CTCGGTG74800.035.72377836-37
CGTCGTG74950.035.6133712-13
Produced by FastQC (version 0.11.2)