##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l01n02 50072-bar8.342000000044d4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13225565
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.43840539137648	38.0	32.0	38.0	32.0	38.0
2	35.6216185849149	38.0	32.0	38.0	32.0	38.0
3	35.55911214379121	38.0	32.0	38.0	32.0	38.0
4	35.88653066995626	38.0	38.0	38.0	32.0	38.0
5	35.787684458093096	38.0	38.0	38.0	32.0	38.0
6	37.746444027154986	40.0	38.0	40.0	32.0	40.0
7	37.97412216415707	40.0	38.0	40.0	38.0	40.0
8	38.08670926346058	40.0	38.0	40.0	38.0	40.0
9	38.117122708935305	40.0	38.0	40.0	38.0	40.0
10-11	38.099150395465145	40.0	38.0	40.0	38.0	40.0
12-13	38.13758482151802	40.0	38.0	40.0	38.0	40.0
14-15	38.08669312048294	40.0	38.0	40.0	38.0	40.0
16-17	38.10413944508231	40.0	38.0	40.0	38.0	40.0
18-19	38.0929516810813	40.0	38.0	40.0	38.0	40.0
20-21	38.06698220454098	40.0	38.0	40.0	38.0	40.0
22-23	38.06353350499582	40.0	38.0	40.0	38.0	40.0
24-25	38.01549778780718	40.0	38.0	40.0	38.0	40.0
26-27	38.014344339920456	40.0	38.0	40.0	38.0	40.0
28-29	37.91879152232816	40.0	38.0	40.0	38.0	40.0
30-31	37.91161852064543	40.0	38.0	40.0	38.0	40.0
32-33	37.89959328769697	40.0	38.0	40.0	38.0	40.0
34-35	37.85011974913738	40.0	38.0	40.0	32.0	40.0
36-37	37.86051253764962	40.0	38.0	40.0	35.0	40.0
38-39	37.8445633135522	40.0	38.0	40.0	35.0	40.0
40-41	37.74148363415854	40.0	38.0	40.0	32.0	40.0
42-43	37.54505701646773	40.0	38.0	40.0	32.0	40.0
44-45	37.4201185733842	40.0	38.0	40.0	32.0	40.0
46-47	37.362120862133295	40.0	38.0	40.0	32.0	40.0
48-49	37.33631270195262	40.0	38.0	40.0	32.0	40.0
50-51	37.260460252548754	39.0	38.0	40.0	32.0	40.0
52-53	37.222306608451134	38.0	38.0	40.0	32.0	40.0
54-55	37.20002555656413	38.0	38.0	40.0	32.0	40.0
56-57	37.14265689216302	38.0	38.0	40.0	32.0	40.0
58-59	37.083608148309736	38.0	38.0	40.0	32.0	40.0
60-61	37.044059025077566	38.0	38.0	40.0	32.0	40.0
62-63	37.02128737033163	38.0	38.0	40.0	32.0	40.0
64-65	36.94731657966975	38.0	38.0	40.0	32.0	40.0
66-67	36.936571254233755	38.0	38.0	40.0	32.0	40.0
68-69	36.88731131713465	38.0	38.0	40.0	32.0	40.0
70-71	36.77760817779807	38.0	38.0	40.0	32.0	40.0
72-73	36.74052382639229	38.0	38.0	40.0	32.0	40.0
74-75	36.63167392092512	38.0	38.0	40.0	32.0	40.0
76-77	36.56567420749133	38.0	38.0	40.0	32.0	40.0
78-79	36.53162878107665	38.0	38.0	40.0	32.0	40.0
80-81	36.44627998879443	38.0	38.0	40.0	32.0	40.0
82-83	36.36063771188603	38.0	38.0	40.0	32.0	40.0
84-85	36.22855144562822	38.0	38.0	40.0	27.0	40.0
86-87	36.14895983649848	38.0	38.0	40.0	27.0	40.0
88-89	36.10638354580693	38.0	38.0	40.0	27.0	40.0
90-91	35.99995803581926	38.0	38.0	40.0	27.0	40.0
92-93	35.9379274533829	38.0	38.0	40.0	27.0	40.0
94-95	35.84523984419569	38.0	38.0	40.0	27.0	40.0
96-97	35.730085519975894	38.0	38.0	40.0	27.0	40.0
98-99	35.620818732507836	38.0	38.0	40.0	27.0	40.0
100-101	33.63388433688844	38.0	32.5	39.0	20.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	-0.03829170387452763
2201	2	-0.08226364832493971
2201	3	-0.10488681152768464
2201	4	-0.02597876603290672
2201	5	0.15575642372643728
2201	6	0.16898056762875058
2201	7	0.12424373945453482
2201	8	0.16608388863897972
2201	9	0.10464055470549027
2201	10-11	0.2133745368912301
2201	12-13	0.18335266486916169
2201	14-15	0.18403477756312725
2201	16-17	0.07203516683918565
2201	18-19	0.06983565157278804
2201	20-21	0.13045508142216278
2201	22-23	0.07252737845038126
2201	24-25	0.0969706380540245
2201	26-27	0.1319651997989837
2201	28-29	0.06583869498565775
2201	30-31	0.10641488400901267
2201	32-33	0.16971665933613878
2201	34-35	0.11819032000904173
2201	36-37	0.15080999894973246
2201	38-39	0.09194694564180139
2201	40-41	0.10978465325412401
2201	42-43	0.05487039679712069
2201	44-45	0.04707991297652825
2201	46-47	0.05681610511842905
2201	48-49	0.010350335529196286
2201	50-51	0.06694232349986606
2201	52-53	0.014374371698707478
2201	54-55	0.01767533700566304
2201	56-57	0.03132315568190336
2201	58-59	-0.013661644405722484
2201	60-61	-0.17357125011923102
2201	62-63	-0.017881126774106804
2201	64-65	-0.03229606938094065
2201	66-67	-0.054920190603631625
2201	68-69	-0.027128005069570804
2201	70-71	-0.09735356184179267
2201	72-73	-0.05628503274867569
2201	74-75	-0.08025672177155485
2201	76-77	-0.04218232212257078
2201	78-79	-0.08160623278029533
2201	80-81	-0.15561520272603957
2201	82-83	-0.14361364598048
2201	84-85	-0.1797069075866844
2201	86-87	-0.06008207131572618
2201	88-89	-0.14374085647532553
2201	90-91	-0.13683231793058326
2201	92-93	-0.13056266399824779
2201	94-95	-0.09985667466823855
2201	96-97	-0.18229571583231063
2201	98-99	-0.19877895455056205
2201	100-101	-0.10036145466057889
1101	1	-0.06394740509477259
1101	2	0.001353480247736627
1101	3	0.03503031474556195
1101	4	0.1137948777051605
1101	5	0.1322075614921232
1101	6	0.17440132229770455
1101	7	0.1600878891623836
1101	8	0.21104917588408512
1101	9	0.24066492672445605
1101	10-11	0.21613914182224647
1101	12-13	0.21364434247418984
1101	14-15	0.22765053959343362
1101	16-17	0.1535857326677359
1101	18-19	0.18871463299797853
1101	20-21	0.21982701063062393
1101	22-23	0.11283579459164628
1101	24-25	0.16891785796846648
1101	26-27	0.16586663242326694
1101	28-29	0.21689497631114563
1101	30-31	0.1961834834441234
1101	32-33	0.20069562124803042
1101	34-35	0.15092836159847423
1101	36-37	0.15048640979991035
1101	38-39	0.17420840006342786
1101	40-41	0.11296912129774483
1101	42-43	0.05310285425396444
1101	44-45	0.07699611928947547
1101	46-47	8.21197742780555E-4
1101	48-49	0.10998069382589648
1101	50-51	0.035091568210937396
1101	52-53	0.09514716092851216
1101	54-55	0.07853696113603092
1101	56-57	0.07810233025966795
1101	58-59	0.09406940069989389
1101	60-61	0.046758710658906466
1101	62-63	0.09473377045821252
1101	64-65	0.05643923607214418
1101	66-67	-0.049422983267319864
1101	68-69	0.010142620614210784
1101	70-71	-0.02891024255968233
1101	72-73	-0.04693811686437499
1101	74-75	-0.022692714938941094
1101	76-77	0.003839363720736344
1101	78-79	0.05241418656813579
1101	80-81	0.007439366619585996
1101	82-83	0.030757708749305834
1101	84-85	0.07117097325299682
1101	86-87	0.12246022509044252
1101	88-89	0.0016521079650857473
1101	90-91	0.03694407795849486
1101	92-93	0.025645644093863496
1101	94-95	-0.07173661818995214
1101	96-97	-0.03346310958665555
1101	98-99	0.008685399793250781
1101	100-101	0.25930246387135725
2202	1	-0.2531455344372091
2202	2	-0.2077454228134883
2202	3	-0.05869874062754832
2202	4	0.05592763213047647
2202	5	-0.005668607419941907
2202	6	-0.08155566062458064
2202	7	0.03587426005826444
2202	8	0.07525561585653406
2202	9	0.08179112044112458
2202	10-11	0.11334104847555437
2202	12-13	0.11818760671556561
2202	14-15	0.10731714808360948
2202	16-17	0.14125288471883835
2202	18-19	0.11334686816915962
2202	20-21	0.1178618642544933
2202	22-23	0.08613539986549057
2202	24-25	0.11703399771572975
2202	26-27	0.04920404192700545
2202	28-29	0.08543741945190675
2202	30-31	0.07133694497012755
2202	32-33	0.11973100377840495
2202	34-35	0.06440607924304942
2202	36-37	0.05938567649822346
2202	38-39	0.06947549701677502
2202	40-41	0.054555044674586384
2202	42-43	-0.07847984050020784
2202	44-45	-0.0760201400934335
2202	46-47	-0.09032805373175279
2202	48-49	-0.07511362560942558
2202	50-51	-0.05973013275034589
2202	52-53	-0.05048756939314103
2202	54-55	-0.04375789585537149
2202	56-57	-0.052725608214139186
2202	58-59	-0.06367865936910988
2202	60-61	-0.14846046550680825
2202	62-63	-0.16086641191956375
2202	64-65	-0.11409856432423737
2202	66-67	-0.21907693672991257
2202	68-69	-0.141318070311506
2202	70-71	-0.1081173849069117
2202	72-73	-0.13514175244112892
2202	74-75	-0.06186117116783407
2202	76-77	-0.1446787757383845
2202	78-79	-0.13263501299349656
2202	80-81	-0.10456805781748102
2202	82-83	-0.14032094884335322
2202	84-85	-0.08394703876631837
2202	86-87	-0.13842443171169094
2202	88-89	-0.10709602861930279
2202	90-91	-0.10847748659626433
2202	92-93	-0.13188113304280336
2202	94-95	-0.1424227132071323
2202	96-97	-0.18379692153167326
2202	98-99	-0.08341346166563568
2202	100-101	0.07171100267390074
1102	1	-0.46299837673777233
1102	2	-0.11802608993288999
1102	3	-0.19650024044144487
1102	4	-0.10359765134735
1102	5	-0.062053240150397926
1102	6	-0.22511618711041592
1102	7	-0.03470554236465517
1102	8	0.021391213678718657
1102	9	0.048217277358745037
1102	10-11	0.08462140933642814
1102	12-13	0.12259103808975169
1102	14-15	0.13921819954042292
1102	16-17	0.08905022383926564
1102	18-19	0.07032636569766737
1102	20-21	0.07656921462178445
1102	22-23	0.06333740320731351
1102	24-25	-3.897608250014173E-4
1102	26-27	0.042842587763026074
1102	28-29	0.07684308940890361
1102	30-31	0.07920342486337262
1102	32-33	0.07889757458901414
1102	34-35	0.028282091823776057
1102	36-37	0.024774496200592466
1102	38-39	0.03834465625951822
1102	40-41	0.03410078204861833
1102	42-43	-0.07739751143003559
1102	44-45	-0.1863129233341141
1102	46-47	-0.13040360934942186
1102	48-49	-0.12057567626373356
1102	50-51	-0.09071133822669708
1102	52-53	-0.15028536917660062
1102	54-55	-0.08845247963812852
1102	56-57	-0.22347036115230878
1102	58-59	-0.21132507467697081
1102	60-61	-0.2204980311493614
1102	62-63	-0.13876266433751994
1102	64-65	-0.24237342692308772
1102	66-67	-0.1846588197880905
1102	68-69	-0.23361884621068185
1102	70-71	-0.129058508220524
1102	72-73	-0.12342729179121648
1102	74-75	-0.1374805662499483
1102	76-77	-0.12695161848742487
1102	78-79	-0.1998594974905359
1102	80-81	-0.14226024536993265
1102	82-83	-0.16374266002098636
1102	84-85	-0.16989539407195053
1102	86-87	-0.09501421622130835
1102	88-89	-0.14802556391916966
1102	90-91	-0.1754827187502528
1102	92-93	-0.2697706748249047
1102	94-95	-0.30727245985803364
1102	96-97	-0.2515162529581332
1102	98-99	-0.20841268410343616
1102	100-101	0.01797336765837798
2203	1	-0.316189908166578
2203	2	-0.33435025718164724
2203	3	-0.29031188906436967
2203	4	-0.1028098499824992
2203	5	-0.16506146677748035
2203	6	-0.2579031548264439
2203	7	-0.06702931703659942
2203	8	0.008391348456292747
2203	9	0.0015621104035901112
2203	10-11	0.08824781457685305
2203	12-13	0.042048655152804315
2203	14-15	-0.025655886104608783
2203	16-17	0.01970144054450884
2203	18-19	0.00318378547899556
2203	20-21	0.013375160496153171
2203	22-23	0.02152512350048852
2203	24-25	0.04173127568995483
2203	26-27	0.06175075837148114
2203	28-29	0.06046871933965292
2203	30-31	0.05531443306623629
2203	32-33	0.09549693162212236
2203	34-35	0.010944130946867858
2203	36-37	0.03974302871138491
2203	38-39	0.038987326052129845
2203	40-41	-0.01720664151560669
2203	42-43	-0.08976554859772534
2203	44-45	-0.19839985988001274
2203	46-47	-0.14009846275638438
2203	48-49	-0.09862683993532784
2203	50-51	-0.1612828033868965
2203	52-53	-0.17621294074889704
2203	54-55	-0.09054351502982882
2203	56-57	-0.12386713559203599
2203	58-59	-0.1826614079999942
2203	60-61	-0.16522387071695732
2203	62-63	-0.16327737073130066
2203	64-65	-0.17542167661982688
2203	66-67	-0.1645845756786528
2203	68-69	-0.12261160628765566
2203	70-71	-0.1824116172181789
2203	72-73	-0.10702022231591002
2203	74-75	-0.06828918603690681
2203	76-77	-0.11716821818252754
2203	78-79	-0.1562526133426232
2203	80-81	-0.1558954461862001
2203	82-83	-0.18844864783700643
2203	84-85	-0.24262509528264076
2203	86-87	-0.09977575002297812
2203	88-89	-0.16889166459360894
2203	90-91	-0.1620814528154284
2203	92-93	-0.2974852727700963
2203	94-95	-0.1405860146842315
2203	96-97	-0.2031992928507762
2203	98-99	-0.2204747975289436
2203	100-101	0.017792809523150765
1103	1	-0.20005167757366848
1103	2	-0.1941718726001369
1103	3	-0.15104150028277985
1103	4	-0.023968348565873043
1103	5	-0.13061403350774015
1103	6	-0.1465976699817091
1103	7	-0.011803792819769399
1103	8	0.0031607234216224356
1103	9	-0.014168959839999218
1103	10-11	0.09661747954988442
1103	12-13	0.06317396716449508
1103	14-15	0.046598471661873475
1103	16-17	0.05246402778362125
1103	18-19	0.011534586188261642
1103	20-21	0.08377196330545189
1103	22-23	0.03789424343880654
1103	24-25	0.06344498229437079
1103	26-27	0.0253825762634321
1103	28-29	0.0017633388599946898
1103	30-31	0.01437969262097738
1103	32-33	0.11621199030925311
1103	34-35	0.042280978955808735
1103	36-37	0.026422259194106346
1103	38-39	-0.045532338794807004
1103	40-41	-0.007624370233777711
1103	42-43	-0.0994907718091298
1103	44-45	-0.11120268908307196
1103	46-47	-0.15936493485573067
1103	48-49	-0.06590017534083614
1103	50-51	-0.10524106149291867
1103	52-53	-0.16491774142258464
1103	54-55	-0.1690097173751539
1103	56-57	-0.22736527161096376
1103	58-59	-0.1489168104750931
1103	60-61	-0.1858425030027817
1103	62-63	-0.08507523067881806
1103	64-65	-0.12597148756204746
1103	66-67	-0.15745646056266338
1103	68-69	-0.12387876842661427
1103	70-71	-0.24330784758395652
1103	72-73	-0.18993733970530968
1103	74-75	-0.1727449929934437
1103	76-77	-0.21459803190131765
1103	78-79	-0.17257364496938266
1103	80-81	-0.19751594796691307
1103	82-83	-0.1833166103860293
1103	84-85	-0.25482838925156415
1103	86-87	-0.17831131056134808
1103	88-89	-0.23191767474681768
1103	90-91	-0.2629276951662831
1103	92-93	-0.33964426292762795
1103	94-95	-0.385293555523468
1103	96-97	-0.36818693585640005
1103	98-99	-0.28399855683608166
1103	100-101	0.01179038191425974
2204	1	-0.307586339107651
2204	2	-0.10765232594955876
2204	3	-0.131270334688935
2204	4	-0.11013730351053397
2204	5	-0.1204767574584622
2204	6	-0.22842757220598742
2204	7	-0.1133622856095684
2204	8	-0.09321970543047087
2204	9	-0.022894727064254994
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2204	12-13	0.004352095662525812
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1113	76-77	-0.2048066591664366
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1113	98-99	-0.3440377967285002
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1112	1	-0.058293829155196875
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1112	9	-0.2149956863966267
1112	10-11	-0.23331637292275786
1112	12-13	-0.22224057567818534
1112	14-15	-0.2473967001938462
1112	16-17	-0.2061719832146096
1112	18-19	-0.3062110017779105
1112	20-21	-0.2642561503234333
1112	22-23	-0.3043656004853901
1112	24-25	-0.2526866163893402
1112	26-27	-0.3127495116482848
1112	28-29	-0.2982985023101534
1112	30-31	-0.2859639704028183
1112	32-33	-0.25589821735020735
1112	34-35	-0.30307904569925626
1112	36-37	-0.3296453118099123
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1112	40-41	-0.27909826572367535
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1112	48-49	-0.22739165833882424
1112	50-51	-0.2137584038256506
1112	52-53	-0.26336821066605864
1112	54-55	-0.1749940768148619
1112	56-57	-0.2897532972012655
1112	58-59	-0.28555393914739824
1112	60-61	-0.28764457350662553
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1112	64-65	-0.45415722003993864
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1112	76-77	-0.2621547373001789
1112	78-79	-0.3160940158564145
1112	80-81	-0.25561780787536037
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1112	84-85	-0.41824773371411794
1112	86-87	-0.25246891144673356
1112	88-89	-0.3856089989632423
1112	90-91	-0.47571887775077926
1112	92-93	-0.4579991088671207
1112	94-95	-0.4630508977327068
1112	96-97	-0.5004125394340733
1112	98-99	-0.46605228153684664
1112	100-101	-0.5994526592875502
1115	1	0.08191816582861833
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1115	3	0.02024310246533645
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1213	12-13	-0.23288000801267117
1213	14-15	-0.16350207277419315
1213	16-17	-0.19022059654567158
1213	18-19	-0.12321431959085771
1213	20-21	-0.11002215975499752
1213	22-23	-0.11624985803954502
1213	24-25	-0.16678891711104882
1213	26-27	-0.16148919084567837
1213	28-29	-0.11237571580002026
1213	30-31	-0.2569264357861485
1213	32-33	-0.18312703839225009
1213	34-35	-0.1746427159550734
1213	36-37	-0.21264618222294018
1213	38-39	-0.22623204041014588
1213	40-41	-0.19273288535924848
1213	42-43	-0.10632861915752301
1213	44-45	-0.02923556359420587
1213	46-47	-0.20029486938886265
1213	48-49	-0.2325435317591129
1213	50-51	-0.28905874054309777
1213	52-53	-0.22236906517507293
1213	54-55	-0.2199305368554647
1213	56-57	-0.1787136182356477
1213	58-59	-0.060150434572712186
1213	60-61	-0.02320596574005407
1213	62-63	-0.12710540851482932
1213	64-65	-0.1320921519148257
1213	66-67	-0.21225236132760728
1213	68-69	-0.061533458445218514
1213	70-71	-0.10432815594589329
1213	72-73	-0.1005365020665181
1213	74-75	-0.09746305758240936
1213	76-77	-0.08843859350128014
1213	78-79	-0.012239126390177546
1213	80-81	-0.048890408768379245
1213	82-83	-0.10732321105801645
1213	84-85	0.024659388830890805
1213	86-87	-0.14126478631360584
1213	88-89	-0.0691596613044041
1213	90-91	-0.054954759632764194
1213	92-93	-0.125314062756253
1213	94-95	-0.25670585109516253
1213	96-97	-0.2566998221813179
1213	98-99	-0.3148338909832873
1213	100-101	-1.1558176840686798
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	20.0
4	49.0
5	78.0
6	79.0
7	101.0
8	93.0
9	112.0
10	125.0
11	255.0
12	292.0
13	305.0
14	597.0
15	3513.0
16	16390.0
17	33201.0
18	39786.0
19	40074.0
20	43470.0
21	50567.0
22	59225.0
23	68303.0
24	77504.0
25	87122.0
26	98703.0
27	110299.0
28	125753.0
29	143241.0
30	164870.0
31	192376.0
32	227371.0
33	277090.0
34	350136.0
35	470502.0
36	695593.0
37	1235659.0
38	3265086.0
39	5347625.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.54747459407163	32.18546119722152	30.415535905013453	19.8515283036934
2	20.65150053959812	33.12276312962222	28.67055108741369	17.555185243365976
3	20.299197482575895	31.318615534729126	29.212445021539857	19.169741961155122
4	20.222044249703377	29.950505721485342	29.63634253967961	20.191107489131667
5	20.053140545269518	30.48991157006693	30.062978193817408	19.39396969084615
6	20.25859894930991	30.683249521466642	28.913169514727517	20.144982014495934
7	20.58972720953741	30.470887650352957	29.496039689611848	19.443345450497784
8	19.792253941057826	30.724812210210217	29.78327500008886	19.699658848643097
9	19.813347781160783	30.30836861166637	30.1452634277269	19.733020179445948
10-11	19.829695193105593	30.646736079122228	29.765391254103196	19.758177473668983
12-13	19.718413865214977	30.547603334571257	30.044520590158523	19.689462210055243
14-15	19.76231400786717	30.527411225047395	30.008690414415035	19.701584352670398
16-17	19.86351576055578	30.298189724452524	30.074750877048622	19.763543637943076
18-19	19.767006579361603	30.183996967752417	30.101656153092655	19.94734029979332
20-21	19.95063370776049	30.237941051817895	29.880577826321563	19.930847414100054
22-23	19.96665065635956	30.13351839735565	29.86386070276424	20.035970243520556
24-25	20.066236281999338	30.069241692494693	29.82537453027696	20.039147495229017
26-27	20.176248082921923	30.054050889547295	29.780492976818294	19.98920805071248
28-29	20.14480520480027	30.09902089477142	29.654195165487586	20.101978734940722
30-31	20.140465042426932	29.940372746078115	29.797041456084024	20.12212075541093
32-33	20.18459570327636	30.038071119266853	29.687670375719083	20.089662801737703
34-35	20.260531930671746	29.98900839674386	29.578262174053354	20.172197498531037
36-37	20.212501540214287	30.021629941175725	29.637928430128124	20.12794008848186
38-39	20.207058263210858	29.90611277949609	29.693973336552855	20.1928556207402
40-41	20.29775655873316	30.010633892696763	29.604288347908298	20.08732120066178
42-43	20.277861107341742	29.969975639528563	29.621776564116086	20.130386689013612
44-45	20.262549005048527	29.920493076000792	29.610769708017422	20.20618821093326
46-47	20.296644924526326	29.970011293043235	29.5561914947325	20.177152287697936
48-49	20.24474865808497	29.89433766705376	29.601382200854136	20.259531474007133
50-51	20.265297201739138	29.972959858670762	29.569423026643875	20.192319912946225
52-53	20.273500804473084	29.99406880032779	29.578627652082627	20.1538027431165
54-55	20.27569859458586	29.93073103725558	29.579126026825563	20.214444341332992
56-57	20.313087856632077	29.991661559775434	29.514765407998883	20.180485175593603
58-59	20.26437633686723	30.040297885868906	29.528843772527715	20.166482004736157
60-61	20.25163402524055	29.97708979943931	29.495797842629084	20.275478332691055
62-63	20.22131484232709	29.942797855072723	29.565426007398287	20.270461295201898
64-65	20.279583212255307	29.990386114981487	29.50949899640752	20.220531676355684
66-67	20.259056390418284	29.968675330394127	29.486206363584827	20.286061915602758
68-69	20.18387310612077	29.94671708438887	29.545087010205474	20.324322799284882
70-71	20.286646586136538	29.983819570071724	29.46643006117612	20.263103782615623
72-73	20.275748429986695	29.98836217615734	29.48437413966175	20.251515254194217
74-75	20.280776282392292	29.961653605128003	29.5088818349483	20.248688277531404
76-77	20.27799149413318	30.008332952293554	29.47091369254246	20.242761861030804
78-79	20.268726164145733	30.000111941816883	29.49412736588652	20.237034528150872
80-81	20.310024290424938	29.975122614133358	29.461395651640654	20.253457443801043
82-83	20.264137342341133	30.06313351454325	29.493199711506353	20.179529431609268
84-85	20.2950785122801	29.99580843210927	29.465065615322693	20.244047440287936
86-87	20.265465527845617	29.990471917156853	29.46964801996408	20.274414535033454
88-89	20.313977948665503	29.969897882919383	29.47146891492886	20.244655253486258
90-91	20.284273116182916	29.982315905986617	29.448078797968773	20.285332179861694
92-93	20.243768181821792	30.00198463198126	29.478479792327384	20.275767393869565
94-95	20.297786066738745	29.975006785590764	29.43489040325321	20.29231674441728
96-97	20.333024298041654	30.022789934300686	29.384350256136166	20.259835511521494
98-99	20.323454877674635	30.019237398055576	29.406397343598222	20.250910380671563
100-101	20.46596992020747	29.95336972090109	29.133864552231113	20.44679580666033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1693.0
1	1751.5
2	2071.0
3	2556.5
4	3101.5
5	3780.5
6	4581.5
7	5389.0
8	6353.0
9	7597.5
10	8964.5
11	10497.5
12	12211.5
13	14270.0
14	16446.0
15	18832.5
16	21987.0
17	26811.5
18	33574.0
19	42909.5
20	55512.5
21	70970.0
22	89448.0
23	111378.0
24	136508.0
25	164565.0
26	195230.0
27	228166.5
28	262299.0
29	298026.0
30	332381.0
31	364155.5
32	395392.0
33	425484.5
34	453621.0
35	479149.0
36	502800.5
37	521332.5
38	529803.5
39	526244.5
40	514514.0
41	495490.5
42	469434.0
43	439699.0
44	410004.5
45	385862.5
46	367377.5
47	352943.0
48	337614.5
49	318376.0
50	297634.5
51	275365.5
52	252479.0
53	231387.5
54	212213.0
55	194438.0
56	178766.0
57	163591.0
58	147045.0
59	128909.0
60	110402.5
61	93586.0
62	77921.0
63	64575.0
64	54034.0
65	45087.5
66	37398.0
67	31041.5
68	25846.5
69	21061.0
70	17207.0
71	14107.5
72	11702.0
73	9733.0
74	8102.0
75	6883.5
76	5886.0
77	5190.0
78	4620.0
79	3949.0
80	3326.0
81	2786.5
82	2399.5
83	2026.5
84	1658.0
85	1353.5
86	1024.0
87	741.0
88	523.0
89	356.5
90	264.0
91	210.0
92	137.0
93	77.5
94	54.5
95	42.5
96	27.5
97	15.5
98	11.5
99	12.5
100	24.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02760562592221958
2	0.027885387127128403
3	0.028701987400916333
4	0.038160940572293134
5	0.021874301778411737
6	0.009859692194624578
7	0.01019994230870288
8	0.018161794978135148
9	0.0073342802367989575
10-11	0.011867167867686561
12-13	0.013579760108547346
14-15	0.03535954796638178
16-17	0.0255490030104574
18-19	0.02789672879759768
20-21	0.013341585028692536
22-23	0.03716287357099678
24-25	0.045918643173278414
26-27	0.03476978110197938
28-29	0.036062731535476934
30-31	0.046807074026705094
32-33	0.04310968945372088
34-35	0.027628309263158134
36-37	0.03782069045821483
38-39	0.04683353792446674
40-41	0.027900509354420774
42-43	0.03755605148059837
44-45	0.05411110980891932
46-47	0.04477691501270456
48-49	0.05682176905107646
50-51	0.053449512364878174
52-53	0.03628956494486247
54-55	0.02741659808106497
56-57	0.03679994011597992
58-59	0.02763965093362741
60-61	0.033117677770288075
62-63	0.029170776446979768
64-65	0.03072080474444759
66-67	0.03173021341621322
68-69	0.0364218844336707
70-71	0.037537148696482905
72-73	0.03040323797130784
74-75	0.03110264098357991
76-77	0.03901912697113507
78-79	0.03335207229331979
80-81	0.037227143036989346
82-83	0.039090957550773825
84-85	0.04663694896966595
86-87	0.047041468549736815
88-89	0.047098176902083205
90-91	0.047945021630455865
92-93	0.04906784700691426
94-95	0.0482172217217185
96-97	0.05300340665975329
98-99	0.05127569219160013
100-101	0.05461014330956749
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.3225565E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.56085681012503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.20307432641582	93.84369924020687
2	1.1904626061107069	2.275232532807071
3	0.22682338527486193	0.650263111243167
4	0.10471938566281884	0.4002829687427556
5	0.04975675417919714	0.23773990307274237
6	0.043659476492652095	0.2503282188911608
7	0.024331921742314022	0.16276255026727068
8	0.01638220735062632	0.12523982166935835
9	0.01602300155389789	0.13780545814444026
>10	0.12449403677292871	1.8842510143219904
>50	2.603310027712294E-4	0.012687501376319434
>100	0.0	0.0
>500	0.0	0.0
>1k	1.2567441416199545E-5	0.019707679256842938
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	6.805002281566043E-5	0.0	7.561113646184492E-6
2	7.561113646184492E-5	0.0	3.780556823092246E-5
3	1.0585559104658289E-4	0.0	5.2927795523291444E-5
4	1.2097781833895188E-4	0.0	6.048890916947594E-5
5	1.2853893198513637E-4	0.0	6.048890916947594E-5
6	1.2853893198513637E-4	0.0	9.07333637542139E-5
7	1.4366115927750533E-4	0.0	1.0585559104658289E-4
8	1.5878338656987432E-4	0.0	1.0585559104658289E-4
9	1.5878338656987432E-4	0.0	1.0585559104658289E-4
10-11	1.9280839797770454E-4	7.561113646184492E-6	1.0585559104658289E-4
12-13	2.3439452303171925E-4	1.890278411546123E-5	1.3610004563132085E-4
14-15	2.532973071471805E-4	2.2683340938553474E-5	1.474417161005976E-4
16-17	2.948834322011952E-4	2.2683340938553474E-5	1.663445002160588E-4
18-19	3.364695572552099E-4	2.6463897761645722E-5	1.9658895480079679E-4
20-21	4.272029210094238E-4	3.780556823092246E-5	2.1927229573935026E-4
22-23	5.06594614294361E-4	4.15861250540147E-5	2.4951675032408826E-4
24-25	5.595224098176524E-4	4.536668187710695E-5	2.8354176173191843E-4
26-27	6.275724326333129E-4	6.048890916947594E-5	2.9866398902428745E-4
28-29	7.561113646184492E-4	6.048890916947594E-5	3.2512788678593313E-4
30-31	8.733086261343088E-4	6.048890916947594E-5	3.704945686630401E-4
32-33	9.753836603577995E-4	6.048890916947594E-5	3.9695846642468586E-4
34-35	0.001107703149166028	6.048890916947594E-5	4.196418073632393E-4
36-37	0.0012211198538587954	6.048890916947594E-5	4.5366681877106953E-4
38-39	0.0013269754449053784	6.048890916947594E-5	4.801307165327152E-4
40-41	0.0013988060245441309	6.048890916947594E-5	4.952529438250841E-4
42-43	0.0014819782746521605	6.048890916947594E-5	5.028140574712687E-4
44-45	0.0016218588771065735	6.048890916947594E-5	5.2171684158673E-4
46-47	0.0018108867182611858	6.048890916947594E-5	5.519612961714679E-4
48-49	0.001954547877538691	6.048890916947594E-5	5.746446371100213E-4
50-51	0.0021435757186933036	6.805002281566043E-5	5.935474212254827E-4
52-53	0.0023477257871402845	6.805002281566043E-5	6.086696485178517E-4
54-55	0.002540534185117989	6.805002281566043E-5	6.351335462794973E-4
56-57	0.0028202953900268155	7.183057963875268E-5	6.578168872180508E-4
58-59	0.0032285955269207777	7.561113646184492E-5	7.334280236798957E-4
60-61	0.0036368956638147404	7.561113646184492E-5	7.712335919108182E-4
62-63	0.0041510513917552855	7.561113646184492E-5	8.014780464955561E-4
64-65	0.004861796074496628	8.317225010802942E-5	8.128197169648329E-4
66-67	0.005837179734854428	9.07333637542139E-5	8.581863988419399E-4
68-69	0.007217082975283097	9.07333637542139E-5	8.77089182957401E-4
70-71	0.009198094750583436	9.829447740039839E-5	8.959919670728624E-4
72-73	0.01171594559476287	1.0585559104658289E-4	9.073336375421391E-4
74-75	0.015262107894823396	1.0585559104658289E-4	9.413586489499693E-4
76-77	0.0201843928784895	1.0585559104658289E-4	9.602614330654304E-4
78-79	0.027038542398755742	1.0585559104658289E-4	9.602614330654304E-4
80-81	0.037079701320888746	1.0585559104658289E-4	9.753836603577995E-4
82-83	0.05092031985022946	1.2097781833895188E-4	0.0010245308990579987
84-85	0.06877588972569414	1.2097781833895188E-4	0.0010547753536427366
86-87	0.09234010040402811	1.2097781833895188E-4	0.0010661170241120133
88-89	0.1241345832862339	1.2097781833895188E-4	0.0010888003650505668
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGTA	365	0.0	16.92118	46-47
TCCGATC	810	0.0	13.195527	32-33
TCGCCGT	545	0.0	11.768223	44-45
GATCGGA	4590	0.0	10.142118	94-95
ATCGGAA	4490	0.0	9.891919	94-95
AGATCGG	4945	0.0	9.846294	94-95
GCCGTAT	825	0.0	8.638085	46-47
AGCGTCG	1520	0.0	8.594179	94-95
CGATCTA	695	3.6379788E-12	8.543414	34-35
TCGGAAG	4260	0.0	8.363093	94-95
TAGATCG	1950	0.0	8.282229	92-93
GACGTGT	1305	0.0	8.189584	20-21
GTCGCCG	910	0.0	8.09215	44-45
TCAGACG	1535	0.0	7.8908257	18-19
CGTCGTG	1395	0.0	7.831932	94-95
CCGATCT	1060	0.0	7.61855	32-33
CCGTATC	1015	0.0	7.4892626	48-49
GCGTCGT	1710	0.0	7.083687	94-95
TGGTCGC	1015	9.094947E-12	6.786772	42-43
CGGAAGA	4140	0.0	6.7696614	94-95
>>END_MODULE